81 results about "Immunoglobulin M" patented technology

The invention belongs to the technical field of analytical chemistry and chemical sensors and discloses an electrochemical immunosensor for detecting a toxoplasma gondii IgM (Immunoglobulin m) antibody (Tg-IgM) of a gravida and a preparation method of the electrochemical immunosensor. The immunosensor is prepared by sequentially modifying graphene, polythionine, gold nanoparticles and capture antigen to the surface of a glassy carbon electrode. An enzyme-functionalized nano-composite detection probe with an electrical signal amplifying function is prepared by assembling enzyme and a second antibody with high proportions on an Au-Fe3O4 surface. According to the sandwich immunoassay principle, the concentration of Tg-IgM is determined by using an electrochemical signal generated by catalysis of enzyme to a substrate. According to the electrochemical immunosensor, the specificity of immunoreaction is combined with the sensitivity of electrochemical detection; the transmission of electronics is promoted by using the graphene, the polythionine, the gold nanoparticles, Au-Fe3O4 and other material; and the sensitivity of the detection is improved. The electrochemical immunosensor has the advantages of simplicity and convenience for operation, favorable regeneration performance and detection cost reduction. The electrochemical immunosensor prepared on the basis can be also used for detecting other immunological markers and has favorable application prospect in medical diagnosis.

The invention provides a nucleic acid molecule. The nucleic acid molecule comprises human immunoglobulin genes or segments thereof and is characterized by also comprising gene segments in a host animal IgM (immunoglobulin M) constant region. The nucleic acid molecule can be used for efficiently preparing full humanized antibodies and has the effect of solving the problem of incompatibility of the interactions between BCRs (B cell receptors) of different species and Ig alpha and Ig beta. Meanwhile, the humanized antibodies expressed by the nucleic acid molecule are unnecessary to undergo second modification.

The invention relates to a herpes simplex virus I and II (HSV-I and HSV-II) immunoglobulin M (IgM) antibody joint inspection kit and a preparation method thereof. The kit comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample absorption pad (4) and a bottom plate (5), wherein the colloidal gold pad is a colloidal gold-labeled mouse anti-human IgM monoclonal antibody glass fiber or a non-woven fabric; and the nitrocellulose membrane is coated with an HSV-I antigen and an HSV-II antigen serving as detection lines, and a goat anti-mouse IgM antibody serving as a quality control line respectively. HSV-I and HSV-II IgM antibodies are detected by a colloidal gold immunochromatography technological principle and can be jointly detected through one-time operation, so that the operation process is simplified, and the kit has the characteristics of quick response, high sensitivity and the like, is easy to operate and is economical and practical.

The invention discloses a preparation method of a biotin marked rabbit anti-tilapia IgM (immunoglobulin m) polyclonal antibody. The preparation method comprises the following steps: collecting tilapia serum, and purifying to obtain purified tilapia IgM; immunizing a rabbit by using the tilapia IgM to obtain a rabbit anti-tilapia IgM polyclonal antibody, and detecting the titer of anti-tilapia serum of the rabbit anti-tilapia IgM polyclonal antibody by adopting ELISA (enzyme linked immunosorbent assay); strengthening the immunity of the purified tilapia IgM, then collecting and separating to obtain serum containing the rabbit anti-tilapia IgM polyclonal antibody, purifying, adding biotin and mixing uniformly to obtain the biotin marked rabbit anti-tilapia IgM polyclonal antibody. The biotin marked rabbit anti-tilapia IgM polyclonal antibody prepared by the method disclosed by the invention is good in specificity and high in purity and titer, and can have a specific binding reaction with the tilapia IgM. The invention also discloses applications of the biotin marked rabbit anti-tilapia IgM polyclonal antibody prepared by the method in ELISA and Western-Blot.

The invention relates to the technical field of biological detection, in particular to a detection method of hemorrhagic fever with renal syndrome IgM (immunoglobulin M) antibodies and a reagent kit. The detection method comprises the steps that hemorrhagic fever with renal syndrome antigens are fixed on a membrane; to-be-detected serum is added; the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum are combined with the antigens fixed on the membrane; gold labeled operating fluid containing mouse anti-human IgM monoclonal antibodies is added; the mouse anti-human IgM monoclonal antibodies are combinec with the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum; a red color is shown; scrubbing liquid is added finally; and the redundant gold labeled operating fluid and other impurities are washed out. The reagent kit has the characteristics of longer quality guarantee period, less cross reaction, better color rendering performance and the like.

The invention discloses test paper. The test paper comprises a sample adding region, a binder region, an observation region and a water absorption region, which are sequentially arranged, wherein the binder region contains antigen combined colloidal gold which can be eluted by a liquid sample; the observation region comprises antibody lines, an antigen line T3 and a quality control line C in sequence from the binder region to the water absorption region; the antibody lines comprise a T1 line and a T2 line; the antibody line T1 is fixedly provided with an anti-human IgG antibody, the antibody line T2 is fixedly provided with an anti-human IgM antibody, the antigen line T3 is fixedly provided with by an antigen, and the quality control line C is fixedly provided with an anti-antigen antibody; and the IgG and the IgM can be specifically combined with the antigen. The test paper can detect whether the tested sample contains IgG and IgM simultaneously one time, can prevent leaked detection, shorten the detection time and reduce the detection expense and can be convenient for field detection.

The invention discloses a method and a kit for performing quick co-detection on anti-Mc (Moraxella catarrhalis) IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies based on magnetic separation and multi-color quantum dot labeling. The kit consists of anti-Mc antibody capturing nano magnetic beads with an anti-Mc IgM and IgG antibody gathering function, anti-human IgM and IgG antibody nano probes labeled by multi-color quantum dots, quality control substances and a PBST buffering solution, wherein the quality control substances comprise a positive quality control substance and a negative quality control substance; the positive quality control substance is serum, in which anti-Mc IgM and IgG antibodies of Mc infected people are respectively positive; the negative quality control substance is serum, in which anti-Mc IgM and IgG are respectively negative. The kit and the method have the advantages of simplicity, quickness and high sensitivity, and can be used for carrying out synchronous detection on the anti-Mc IgM and IgG antibodies.

The invention relates to an ELISA (enzyme-linked immuno sorbent assay) detection kit for porcine parvovirus IgM (immunoglobulin m) antibodies as well as a preparation method and application of the ELISA detection kit. The ELISA detection kit for the porcine parvovirus IgM antibodies comprises an ELISA plate coating a porcine IgM monoclonal antibody, sealing fluid, a sample diluent, a detection antigen, an enzyme conjugate, a concentrated cleaning solution, a zymolyte solution A, a zymolyte solution B and a stop solution, wherein the detection antigen is a purified porcine parvovirus, and the enzyme conjugate is a horse-radish peroxide enzyme-anti-PPV (porcine parvovirus) antibody enzyme conjugate. The specificity of the ELISA detection kit provided by the invention reaches 100% and the sensitivity of the ELISA detection kit reaches up to 1:800, so that the ELISA detection kit can be used for performing early diagnosis on PPV infection of a swinery.

The invention discloses fluorescent quantitative PCR (Polymerase Chain Reaction) primers for detecting mycoplasma pneumoniae (MP) and application thereof. A primer pair provided by the invention is specific to a 16SrRNA gene or ATPase gene of MP and can be a primer pair 1 (shown by a sequence 1 and a sequence 2) or a primer pair 2 (shown by a sequence 3 and a sequence 4) or a primer pair 3 (shown by a sequence 5 and a sequence 6). Shown by experiments, compared with other commercialized detection primers, IgM (Immunoglobulin M) antibody detection methods and culture methods, a method in which the primer pair provided by the invention can be used for carrying out fluorescent quantitative PCR detection on test samples has the advantage that the MP can be specifically detected without being interfered by four kinds of common pathogenic mycoplasmas which have a relatively close genetic relationship with the MP, as well as common respiratory tract bacteria and fungal pathogenic bacteria. The method can be used for qualitatively detecting the MP and detecting the MP quantitatively very well, is a quantitative PCR detection technology which is quick, is strong in specificity and high in sensitivity and is suitable for clinically detecting the MP of all strains, and has good detection effect and application value in clinical detection.