51 results about "Immunoglobulin M" patented technology

The invention relates to the technical field of molecular biological detection, in particular to an SNP molecular marker related to immune traits of yaks and application of the SNP molecular marker. According to the invention, the SNP molecular marker related to the immune traits of yaks is obtained through screening and is located at the 55577127th position on the 22 # chromosome of a yak reference genome LUBosgruv3.0 version, and a mutant basic group is G or A. The content of immune globulin A, immune globulin G and immune globulin M of a yak individual can be obtained by utilizing the SNP molecular marker, a new SNP molecular marker resource is provided for non-diagnostic yak immune character marker-assisted selection, and a basis is provided for high-immunity yak breeding.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a molecular marker related to immune indexes of yaks and application of the molecular marker in breeding. The SNP molecular marker related to the immune traits of the yak is obtained through screening and is located at the 26593056th basic group of chromosome 3 of a yak reference genome LUBosgruv3.0 version, and the mutant basic group is G or A. The content of immune globulin A, immune globulin G and immune globulin M of a yak individual can be obtained by utilizing the SNP molecular marker, a new SNP molecular marker resource is provided for non-diagnostic yak immune character marker-assisted selection, and a basis is provided for high-immunity yak breeding.

A lyophilized composition including polyclonal immunoglobulin M (IgM), including the steps of: a) providing an initial aqueous solution including IgM at a concentration between about 10 mg / mL and about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content of the composition; b) adding amino acids selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline or a mixture thereof at a final concentration of about 0.15 M to about 0.45 M; polysorbate 80 (PS80) at a concentration between about 50 and about 200 ppm; and succinic acid at a concentration between about 1 mM and about 20 mM; and c) lyophilizing the IgM composition obtained in step b); wherein the lyophilized composition has a content of pentameric IgM higher than about 90% of the total IgM content, a content of IgM aggregates of less than about 1.5%.

The invention belongs to the technical field of molecular biological detection, and particularly relates to cloning of an SNP molecular marker related to immune traits of yaks and application of the SNP molecular marker. The SNP molecular marker related to the immune traits of the yak is obtained through screening and located at the 35789017th basic group on the 28th chromosome of a yak reference genome LUBosgruv3.0, and the mutant basic group is C or T. The molecular marker can judge the content of immune globulin M of an individual yak. The invention provides a new SNP molecular marker resource for non-diagnostic yak immune trait marker-assisted selection, and provides a basis for high-immunity yak breeding.

The invention discloses application of bacillus subtilis QST713 in improving the production performance and reproduction traits of Xiaoshan chickens. The production performance of the Xiaoshan chickens is improved by any one or more than two of the following steps: (1) reducing the feed-egg ratio of the Xiaoshan chickens; (2) the egg weight of Xiaoshan chicken is increased; (3) the egg protein height of Xiaoshan chicken is increased; (4) increasing any one or more of total protein, globulin and albumin in serum of the Xiaoshan chicken; (5) reducing any one or more of cholesterol, triglyceride, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and low-density lipoprotein in serum of the Xiaoshan chicken; and (6) increasing any one or more of immune globulin A, immune globulin G and immune globulin M in the serum of the Xiaoshan chicken. By adding the bacillus subtilis QST713 strain, the feed-egg ratio of the Xiaoshan chicken can be remarkably reduced, the egg quality and serum biochemical indexes of the Xiaoshan chicken can be effectively improved, the immune function is enhanced, and the ovarian gene expression of the Xiaoshan chicken is influenced.

The invention discloses a lipidosome drug delivery system for targeted therapy of brain glioma and application thereof. The lipidosome drug delivery system is a lipidosome mixture prepared from a basic component, a functional component and an external water-phase buffer solution. After intravenous injection, the liposome is efficiently combined with complement protein C3 and immune globulin M in plasma to synergistically activate a complement system, actively attracts and activates neutrophils to uptake, and crosses a blood brain barrier in a mode of'building a defecation vehicle 'by utilizing the inflammatory taxis of the neutrophils. Neutrophile granulocytes are stimulated by inflammatory factors in a microenvironment after reaching a tumor brain area to form extracellular trap release lipidosome. The lipidosome realizes specific targeting through folate receptors over-expressed on the surfaces of glioma cells. The liposome shows excellent cross-blood-brain-barrier drug delivery efficiency and brain glioma curative effect in vivo, can be effectively combined with surgery, radiotherapy and the like, and provides a solution for the main problems of radiotherapy resistance, chemotherapy drug resistance and the like in clinic.

The invention relates to the technical field of biological detection, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for influencing the content of yak immunoglobulin M and application. According to the scheme, the SNP molecular marker for detecting the content of the yak immune globulin M is located at the 42437268th basic group on the No.15 chromosome of a yak reference genome LUBosgruv3.0 version, and the mutant basic group is A or G; according to molecular marker alleles, yaks have three genotypes of AA, AG or GG. The invention provides a new SNP molecular marker resource for non-diagnostic yak immune trait marker-assisted selection, and provides a basis for yak assisted breeding.

Provided herein are immunoglobulin M (IgM) scaffolds as well as protein complexes comprising both an IgM scaffold and one or more single-domain antigen-binding fragment (sdAb) conjugates. Also provided are methods of using the protein complexes disclosed herein for the treat of disease, including for the treatment of infection or cancer.

The invention relates to the technical field of antibody detection, and discloses a canine entomophilous disease quintuplet antibody detection card, a preparation method thereof and a kit. The detection card comprises the following five detection antigens a)-e): a) a leishmania antigen which is fusion protein of rk39, rk26 and rk9; b) a babesia antigen which is a fusion protein of TRAP and rBcMSA1 / rBcSA1; c) an amorphous antigen which is a fusion protein of P44 and OMP-1X; the detection card and the kit have the advantages that joint detection of IgG (immunoglobulin G) and IgM (immunoglobulin M) antibodies corresponding to canine Ehrlichia, canine anaplasmosis, canine babesiosis, canine lyme disease and canine leishmania can be realized by the detection card and the kit, and the detection sensitivity and the specificity are high. The detection card and the kit can be used for detecting canine Ehrlichia, canine anaplasmosis, canine babesiosis, canine lyme disease and canine leishmania. The detection card and the kit have the advantages that the detection card and the kit can be used for detecting canine Ehrlichia, canine anaplasmosis, canine babesiosis and canine leishmania;

The present invention belongs to the technical field of molecular biological detection, and in particular to a molecular marker g8087186 for rapid identification of Tibetan sheep immunity and its application. The present invention obtains a SNP molecular marker g8087186 related to Tibetan sheep immune traits by screening, which is located at the 8087186th base on chromosome 3 of the sheep reference genome group Oar_v4.0 version, and the mutant base is G or C. The SNP molecular marker can be used to obtain the content of immunoglobulin A, immunoglobulin G, and immunoglobulin M of Tibetan sheep individuals, and provides a new SNP molecular marker resource for marker-assisted selection of Tibetan sheep immune traits for non-diagnostic purposes. Provide a basis for breeding Tibetan sheep with high immunity.

The invention provides a construction method and application of an IgM gene knockout zebrafish antibody deletion model, and belongs to the technical field of zebrafish model construction.The method is based on a CRISPR gene knockout technology and specifically comprises the following operation steps that gRNA is synthesized for a CRISPR target located in a CH2 exon region of a zebrafish IgM gene, the gRNA and Cas9 mRNA / Cas9 protein are introduced into a wild type zebrafish embryo, and the zebrafish antibody deletion model is obtained. The P0-generation mutant and wild type zebra fish are hybridized to generate a heterozygote F1 generation, and then the heterozygote F1 generation is selfed to obtain a homozygote mutant, namely the IgM gene knockout zebra fish antibody deletion model. The invention provides the first IgM gene knockout zebrafish antibody deletion model, which is beneficial to exploring the biological function of the IgM gene and determining the influence of immunoglobulin antibody M synthesis disorder on the immune response of the zebrafish, and has application value for rapidly screening and evaluating vaccine adjuvant drugs and natural efficacy factors.

The utility model discloses a test card structure which comprises test paper arranged in a lower shell through a fixing assembly, and an IgM (Immunoglobulin M) detection line and an IgG (Immunoglobulin G) detection line are arranged in a detection area of the test paper; the upper inserting column and the upper inserting sleeve are arranged on the top surface of the inner cavity of the upper shell and are respectively distributed on two sides of the inner cavity of the upper shell in the width direction; the bottom inserting sleeve and the bottom inserting column are arranged on the bottom face of an inner cavity of the lower shell, the bottom inserting sleeve is matched with the upper inserting column in an inserting mode, and the bottom inserting column is matched with the upper inserting sleeve in an inserting mode; and the positioning notches are arranged at one ends of the upper shell and the lower shell in the length direction. According to the scheme, the upper shell and the lower shell are assembled through insertion of the upper insertion columns and the bottom insertion sleeves and insertion of the upper insertion sleeves and the bottom insertion columns, through distribution of the upper insertion columns and the upper insertion sleeves, the upper shell and the lower shell are assembled in a unique mode, the upper shell and the lower shell are matched with the positioning notches, and wrong assembly of the upper shell and the lower shell can be avoided.

The invention belongs to the technical field of biology, and discloses a preparation method and application of a shark nano antibody targeting IgM (Immunoglobulin M). The shark nano antibody is characterized in that the amino acid sequences of the shark nano antibody are 1-1D, 1-3G and 1-5E as shown in SEQ ID NO. 1-SEQ ID NO. 3. Compared with the traditional antibody, the VNAR prepared by the invention has the characteristics of small molecular weight, strong physical and chemical stability, low production cost and the like. The invention provides a preparation method of targeted IgM, namely immunizing sharks in the earlier stage, then constructing a phage display library with high storage capacity, performing three rounds of panning by utilizing a phage display technology to obtain high-affinity VNAR, finally purifying to obtain three VNAR proteins, and detecting that the IgM and the VNAR can be effectively combined by BLI. The screened VNAR can be used as a raw material of an ELISA detection kit, a colloidal gold method detection kit and the like, and a foundation is laid for early diagnosis of diseases.

The present invention belongs to the field of molecular biological detection technology, and in particular, relates to a molecular marker-assisted breeding method for Tibetan sheep with strong disease resistance. The present invention obtains a molecular marker for assisting the breeding of Tibetan sheep with strong disease resistance through screening, and the molecular marker is located at the 164719075th base on chromosome 3 of the sheep reference genome Oar_v4.0 version, and the mutant base is T or C. The molecular marker can be used to obtain the content of immunoglobulin A, immunoglobulin G, and immunoglobulin M of Tibetan sheep individuals, and provides a new molecular marker resource for marker-assisted selection of Tibetan sheep immune traits for non-diagnostic purposes. Provide a basis for breeding Tibetan sheep with high immunity.

The invention relates to application of aesculetin in preparation of a preparation for improving the health condition of poultry animals. The improved poultry animal abnormal indexes caused by heat stress comprise body weight, feed intake, daily gain, thymus index, spleen index, bursa of Fabricius index, thymus volume, spleen volume, bursa of Fabricius volume, total protein, serum albumin, interleukin-10, immune globulin A, immune globulin G, immune globulin M, triglyceride, total cholesterol and glutamic-pyruvic transaminase. The vaccine is prepared from the following raw materials: alkaline phosphatase, glutamic oxalacetic transaminase, urea nitrogen, lactic dehydrogenase, creatinine, heat shock protein 70, interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, interleukin-gamma, MyD88, TLR4, RELA, CD3 and CD19.

The present invention relates to the field of molecular biology detection technologies, and particularly to SNP molecular markers related to the immune traits of yaks and their applications. The present invention screens out SNP molecular markers related to the immune traits of yaks, which are located at position 55577127 on chromosome 22 of the yak reference genome LU_Bosgru_v3.0 version, and the mutated base is G or A. The present invention can obtain the contents of immunoglobulin A, immunoglobulin G, and immunoglobulin M of yak individuals by using this SNP molecular marker, providing a new SNP molecular marker resource for marker-assisted selection of yak immune traits for non-diagnostic purposes and providing a basis for breeding yaks with high immunity.

A lyophilized composition comprising a polyclonal immunoglobulin M (IgM) comprising the steps of: a) providing an initial aqueous solution comprising IgM at a concentration of from about 10 mg / mL to about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content in the composition; b) adding an amino acid selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline, or mixtures thereof, at a final concentration of from about 0.15 M to about 0.45 M, polysorbate 80 (PS80) at a concentration of from about 50 to about 200 ppm, and succinic acid at a concentration of from about 1 mM to about 20 mM; and c) lyophilizing the IgM composition obtained in the step b), wherein the content of pentamer IgM in the lyophilized composition is greater than about 90% of the total IgM content, and the content of IgM aggregate is less than about 1.5%.

The invention discloses a detection test strip and a detection card for detecting a coxsackie virus group B IgM antibody as well as a preparation method and application of the detection test strip and the detection card. The detection test strip comprises a bottom plate as well as a nitrocellulose membrane, absorbent paper, a conjugate pad and a sample pad which are sequentially overlapped and fixed on the bottom plate, the nitrocellulose membrane is coated with a detection line and a quality control line, the detection line is coated with an antigen of an anti-coxsackie virus group B IgM antibody, and the quality control line is coated with an IgG polyclonal antibody; the conjugate pad comprises an antigen conjugate of a colloidal gold-anti-coxsackie virus group B IgM (Immunoglobulin M) antibody; the particle size of the colloidal gold is larger than or equal to 100 nm. The detection test strip prepared by the invention is high in sensitivity, good in repeatability and strong in specificity, and can be used for detecting the coxsackie virus group B IgM antibody. The device is convenient to use, easy to operate, short in consumed time and convenient to carry.

A molecular marker associated with immune parameters in yaks and use thereof in breeding are provided. A single nucleotide polymorphism (SNP) molecular marker associated with immune traits in yaks is identified through screening. The SNP molecular marker is located at position 26593056 on chromosome 3 of the yak reference genome version LU_Bosgru_v3.0, and a nucleotide at the position is G or A. The SNP molecular marker is utilized to quantify levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M in a yak individual, providing a novel SNP molecular marker resource for non-diagnostic, marker-assisted selection of immune traits in yaks, thereby providing a basis for breeding yaks with enhanced immune competence.

A lyophilized composition including polyclonal immunoglobulin M (IgM), including the steps of: a) providing an initial aqueous solution including IgM at a concentration between about 10 mg / mL and about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content of the composition; b) adding amino acids selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline or a mixture thereof at a final concentration of about 0.15 M to about 0.45 M; polysorbate 80 (PS80) at a concentration between about 50 and about 200 ppm; and succinic acid at a concentration between about 1 mM and about 20 mM; and c) lyophilizing the IgM composition obtained in step b); wherein the lyophilized composition has a content of pentameric IgM higher than about 90% of the total IgM content, a content of IgM aggregates of less than about 1.5%.

The present invention discloses a composite formulation for fasting-induced physiological remodeling in laying hens and its application, belonging to the technical field of fasting-induced physiological remodeling. The composite formulation and method provided by the present invention can significantly increase the ileal villus height, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes of laying hens, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens; can significantly increase the expression levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M, and thus improve the immune performance of laying hens; with the improvement of intestinal health and immunity, fasting-induced physiological remodeling of laying hens is successfully achieved: the weight and egg production recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical support for the extended breeding of laying hens, saving costs and increasing efficiency.

Provided is an adapter comprising an antibody binding domain (AbBD) that specifically binds and crosslinks to an immunoglobulin Fab region, where the AbBD is a variant of a Protein A domain D and has an amino acid replacement / modification at A28, G32, Q35, S36, N46, G49, E50, K53 or L54 of SEQ ID NO: 1 or combinations thereof, and where the AbBD is photoreactive. Also provided is an adapter comprising an AbBD that specifically binds and crosslinks to an IgG Fab region and / or to an immunoglobulin M (IgM) Fab region, where the AbBD is a variant of a Protein A domain D and has an amino acid replacement / modification at A28, E50, or L54 of SEQ ID NO: 1 or combinations thereof, where the AbBD is photoreactive. Provided is an adapter comprising an AbBD that specifically binds and crosslinks to an IgG Fab region, where the AbBD is a variant of a Protein L C* domain and has an amino acid replacement / modification at G22 or T46 of SEQ ID NO: 2 or a combination thereof, wherein the AbBD is photo-reactive. Methods for using the AbBD for imaging, diagnosing and / or treating a disease are provided.

The invention discloses a method for identifying glycosylation modification of immunoglobulin M, and belongs to the technical field of protein analysis. The method comprises the following steps: (1) separating IgM from a sample; (2) preparation and enrichment of IgM glycopeptide; and (3) detecting the IgM glycopeptide by mass spectrometry. Two-dimensional electrophoretic separation is carried out on blood / plasma and other samples containing IgM by combining native-PAGE gel electrophoresis and SDS-PAGE gel electrophoresis, the separation method is mild, the glycosylation rate of the obtained IgM is high, and a good foundation is laid for high-coverage identification of subsequent glycosylation modification; by optimizing the enrichment conditions and elution conditions of the modified polydopamine magnetic nanomaterial, high-coverage enrichment of glycopeptides at five N-glycosylation sites of IgM is realized, and the method has great significance in early warning of immune inflammatory diseases, disease progress monitoring, prognosis evaluation and the like.

The invention provides a method for removing IgA (Immunoglobulin A) and IgM (Immunoglobulin M) in human immunoglobulin for intravenous injection. The method comprises the following steps: S1) dissolving plasma component II + III precipitates to obtain a dissolved solution; s2) precipitating and centrifuging the dissolved solution by adopting n-caprylic acid or sodium caprylate to obtain supernate; s3) performing anion exchange column chromatography on the supernate to obtain first flow-through liquid and first washing liquid; s4) mixing the first flow-through liquid with the first washing liquid to obtain a column chromatography collection liquid; s5) performing strong anion membrane chromatography on the column chromatography collection liquid to obtain second flow-through liquid and second washing liquid; s6) mixing the second flow-through liquid and the second washing liquid to obtain a membrane chromatography collection liquid, and S7) sequentially performing first concentration, dialysis, first sterilization, incubation, ultrafiltration and second sterilization on the membrane chromatography collection liquid to obtain the human immunoglobulin for intravenous injection without IgA and IgM. According to the method disclosed by the invention, the IgA and IgM removal efficiency is remarkably improved through a multi-stage purification process.

This invention provides an immunological assay method capable of suppressing nonspecific reactions caused by immunoglobulin M, abbreviated as IgM, contained in a sample for measurement, and reagents used in this immunological assay method. [Solution] The present invention provides an immunological assay method for measuring a target substance in a sample, comprising performing an antigen-antibody reaction at least once in the presence of an enzyme that specifically degrades IgM, and an immunological assay reagent used in the immunological assay method, comprising an enzyme that specifically degrades IgM.

The invention relates to the field of biological detection, in particular to an IgM antibody for preventing red blood cell agglutination and a preparation method and application thereof, and the IgM antibody for preventing red blood cell agglutination is obtained by mixing and incubating a natural IgM antibody and DTT. The IgM antibody can be used for preparing a product for detecting the ABO blood type and a product for detecting the expression quantity of an A-type antigen and / or a B-type antigen, and the prepared product can be used for quantitatively detecting the expression of the ABO antigen, researching the change condition of the expression quantity of different genotypes of ABO antigens, researching diseases related to the expression quantity of the ABO antigens and the like.

A method of preventing or treating or controlling a respiratory disease in a bovine by delivering to a lung or trachea of the bovine a composition including a polyplex having one or more mRNA encoded with a molecule selected from lycosylphosphatidylinositol (GPI) complex, antibody immunoglobulin G (IgG), antibody immunoglobulin A (IgA), antibody immunoglobulin M (IgM), antimicrobial peptide Bactenecin 5 (Bac5), antimicrobial peptide Bactenecin 7 (Bac7), bovine myeloid antimicrobial peptide (BMAP-28), lipoxin inducing enzymes, resolvin inducing enzymes, cytokines, CRISPR associated protein 13 (Cas13) enzymes, nanoluciferase (NLuc) proteins, and gene activator, catalytically dead CRISPR-associated protein 9 fused to an activator selected from the group consisting of VP64-p65-Rta transactivation domain (dCas9-VPR), VP64, and VP64-p65-HSF1 on the N terminus and SS18 on the C-terminus, and a polymer prepared by polymerizing a diacrylate monomer; one or more linker monomers; and one or more branching monomers; and end-capping the polymer.

An immunological measurement method for measuring a measurement target substance in a sample, wherein an antigen-antibody reaction is conducted at least once or more in the presence of an enzyme that specifically degrades Immunoglobulin M, abbreviated as IgM.

The present invention belongs to the field of molecular biological detection technology, and more particularly relates to a molecular marker associated with immune indicators in Tibetan sheep and its application in breeding. The present invention screens and identifies a single-nucleotide polymorphism (SNP) molecular marker associated with immune traits in Tibetan sheep. The SNP marker is located at base 12,065,580 on chromosome 1 of the sheep reference genome Oar_v4.0, with the mutated base being either C or T. This SNP molecular marker can be used to determine the levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M in individual Tibetan sheep, providing a new SNP molecular marker resource for marker-assisted selection of immune traits in Tibetan sheep for non-diagnostic purposes. This provides a basis for breeding Tibetan sheep with high immunity.

The invention belongs to the field of pharmaceutical preparations, and provides a drug sustained-release capsule for treating liver diseases as well as a preparation method and application of the drug sustained-release capsule. The sustained-release capsule provided by the invention is prepared by filling a capsule with sustained-release pellets. The drug carried by the sustained release pellet is IMM-H014 (Immunoglobulin M-H014). The sustained-release pellet is prepared by coating a sustained-release layer on a drug-loaded pellet, and the drug-loaded pellet has the characteristic of delayed release, and is stable in dissolution rate and good in bioavailability. The traditional Chinese medicine composition is used for treating non-alcoholic fatty liver, hepatitis and drug-induced liver diseases.