35 results about "Immunoglobulin M" patented technology

The invention discloses a lipidosome drug delivery system for targeted therapy of brain glioma and application thereof. The lipidosome drug delivery system is a lipidosome mixture prepared from a basic component, a functional component and an external water-phase buffer solution. After intravenous injection, the liposome is efficiently combined with complement protein C3 and immune globulin M in plasma to synergistically activate a complement system, actively attracts and activates neutrophils to uptake, and crosses a blood brain barrier in a mode of'building a defecation vehicle 'by utilizing the inflammatory taxis of the neutrophils. Neutrophile granulocytes are stimulated by inflammatory factors in a microenvironment after reaching a tumor brain area to form extracellular trap release lipidosome. The lipidosome realizes specific targeting through folate receptors over-expressed on the surfaces of glioma cells. The liposome shows excellent cross-blood-brain-barrier drug delivery efficiency and brain glioma curative effect in vivo, can be effectively combined with surgery, radiotherapy and the like, and provides a solution for the main problems of radiotherapy resistance, chemotherapy drug resistance and the like in clinic.

The invention relates to the technical field of biological detection, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for influencing the content of yak immunoglobulin M and application. According to the scheme, the SNP molecular marker for detecting the content of the yak immune globulin M is located at the 42437268th basic group on the No.15 chromosome of a yak reference genome LUBosgruv3.0 version, and the mutant basic group is A or G; according to molecular marker alleles, yaks have three genotypes of AA, AG or GG. The invention provides a new SNP molecular marker resource for non-diagnostic yak immune trait marker-assisted selection, and provides a basis for yak assisted breeding.

Provided herein are immunoglobulin M (IgM) scaffolds as well as protein complexes comprising both an IgM scaffold and one or more single-domain antigen-binding fragment (sdAb) conjugates. Also provided are methods of using the protein complexes disclosed herein for the treat of disease, including for the treatment of infection or cancer.

The invention provides a construction method and application of an IgM gene knockout zebrafish antibody deletion model, and belongs to the technical field of zebrafish model construction.The method is based on a CRISPR gene knockout technology and specifically comprises the following operation steps that gRNA is synthesized for a CRISPR target located in a CH2 exon region of a zebrafish IgM gene, the gRNA and Cas9 mRNA / Cas9 protein are introduced into a wild type zebrafish embryo, and the zebrafish antibody deletion model is obtained. The P0-generation mutant and wild type zebra fish are hybridized to generate a heterozygote F1 generation, and then the heterozygote F1 generation is selfed to obtain a homozygote mutant, namely the IgM gene knockout zebra fish antibody deletion model. The invention provides the first IgM gene knockout zebrafish antibody deletion model, which is beneficial to exploring the biological function of the IgM gene and determining the influence of immunoglobulin antibody M synthesis disorder on the immune response of the zebrafish, and has application value for rapidly screening and evaluating vaccine adjuvant drugs and natural efficacy factors.

The utility model discloses a test card structure which comprises test paper arranged in a lower shell through a fixing assembly, and an IgM (Immunoglobulin M) detection line and an IgG (Immunoglobulin G) detection line are arranged in a detection area of the test paper; the upper inserting column and the upper inserting sleeve are arranged on the top surface of the inner cavity of the upper shell and are respectively distributed on two sides of the inner cavity of the upper shell in the width direction; the bottom inserting sleeve and the bottom inserting column are arranged on the bottom face of an inner cavity of the lower shell, the bottom inserting sleeve is matched with the upper inserting column in an inserting mode, and the bottom inserting column is matched with the upper inserting sleeve in an inserting mode; and the positioning notches are arranged at one ends of the upper shell and the lower shell in the length direction. According to the scheme, the upper shell and the lower shell are assembled through insertion of the upper insertion columns and the bottom insertion sleeves and insertion of the upper insertion sleeves and the bottom insertion columns, through distribution of the upper insertion columns and the upper insertion sleeves, the upper shell and the lower shell are assembled in a unique mode, the upper shell and the lower shell are matched with the positioning notches, and wrong assembly of the upper shell and the lower shell can be avoided.

The present invention belongs to the field of molecular biological detection technology, and in particular, relates to a molecular marker-assisted breeding method for Tibetan sheep with strong disease resistance. The present invention obtains a molecular marker for assisting the breeding of Tibetan sheep with strong disease resistance through screening, and the molecular marker is located at the 164719075th base on chromosome 3 of the sheep reference genome Oar_v4.0 version, and the mutant base is T or C. The molecular marker can be used to obtain the content of immunoglobulin A, immunoglobulin G, and immunoglobulin M of Tibetan sheep individuals, and provides a new molecular marker resource for marker-assisted selection of Tibetan sheep immune traits for non-diagnostic purposes. Provide a basis for breeding Tibetan sheep with high immunity.

The invention relates to application of aesculetin in preparation of a preparation for improving the health condition of poultry animals. The improved poultry animal abnormal indexes caused by heat stress comprise body weight, feed intake, daily gain, thymus index, spleen index, bursa of Fabricius index, thymus volume, spleen volume, bursa of Fabricius volume, total protein, serum albumin, interleukin-10, immune globulin A, immune globulin G, immune globulin M, triglyceride, total cholesterol and glutamic-pyruvic transaminase. The vaccine is prepared from the following raw materials: alkaline phosphatase, glutamic oxalacetic transaminase, urea nitrogen, lactic dehydrogenase, creatinine, heat shock protein 70, interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, interleukin-gamma, MyD88, TLR4, RELA, CD3 and CD19.

The present invention relates to the field of molecular biology detection technologies, and particularly to SNP molecular markers related to the immune traits of yaks and their applications. The present invention screens out SNP molecular markers related to the immune traits of yaks, which are located at position 55577127 on chromosome 22 of the yak reference genome LU_Bosgru_v3.0 version, and the mutated base is G or A. The present invention can obtain the contents of immunoglobulin A, immunoglobulin G, and immunoglobulin M of yak individuals by using this SNP molecular marker, providing a new SNP molecular marker resource for marker-assisted selection of yak immune traits for non-diagnostic purposes and providing a basis for breeding yaks with high immunity.

A lyophilized composition comprising a polyclonal immunoglobulin M (IgM) comprising the steps of: a) providing an initial aqueous solution comprising IgM at a concentration of from about 10 mg / mL to about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content in the composition; b) adding an amino acid selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline, or mixtures thereof, at a final concentration of from about 0.15 M to about 0.45 M, polysorbate 80 (PS80) at a concentration of from about 50 to about 200 ppm, and succinic acid at a concentration of from about 1 mM to about 20 mM; and c) lyophilizing the IgM composition obtained in the step b), wherein the content of pentamer IgM in the lyophilized composition is greater than about 90% of the total IgM content, and the content of IgM aggregate is less than about 1.5%.

The invention discloses a detection test strip and a detection card for detecting a coxsackie virus group B IgM antibody as well as a preparation method and application of the detection test strip and the detection card. The detection test strip comprises a bottom plate as well as a nitrocellulose membrane, absorbent paper, a conjugate pad and a sample pad which are sequentially overlapped and fixed on the bottom plate, the nitrocellulose membrane is coated with a detection line and a quality control line, the detection line is coated with an antigen of an anti-coxsackie virus group B IgM antibody, and the quality control line is coated with an IgG polyclonal antibody; the conjugate pad comprises an antigen conjugate of a colloidal gold-anti-coxsackie virus group B IgM (Immunoglobulin M) antibody; the particle size of the colloidal gold is larger than or equal to 100 nm. The detection test strip prepared by the invention is high in sensitivity, good in repeatability and strong in specificity, and can be used for detecting the coxsackie virus group B IgM antibody. The device is convenient to use, easy to operate, short in consumed time and convenient to carry.

A molecular marker associated with immune parameters in yaks and use thereof in breeding are provided. A single nucleotide polymorphism (SNP) molecular marker associated with immune traits in yaks is identified through screening. The SNP molecular marker is located at position 26593056 on chromosome 3 of the yak reference genome version LU_Bosgru_v3.0, and a nucleotide at the position is G or A. The SNP molecular marker is utilized to quantify levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M in a yak individual, providing a novel SNP molecular marker resource for non-diagnostic, marker-assisted selection of immune traits in yaks, thereby providing a basis for breeding yaks with enhanced immune competence.

The present invention discloses a composite formulation for fasting-induced physiological remodeling in laying hens and its application, belonging to the technical field of fasting-induced physiological remodeling. The composite formulation and method provided by the present invention can significantly increase the ileal villus height, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes of laying hens, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens; can significantly increase the expression levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M, and thus improve the immune performance of laying hens; with the improvement of intestinal health and immunity, fasting-induced physiological remodeling of laying hens is successfully achieved: the weight and egg production recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical support for the extended breeding of laying hens, saving costs and increasing efficiency.

Provided is an adapter comprising an antibody binding domain (AbBD) that specifically binds and crosslinks to an immunoglobulin Fab region, where the AbBD is a variant of a Protein A domain D and has an amino acid replacement / modification at A28, G32, Q35, S36, N46, G49, E50, K53 or L54 of SEQ ID NO: 1 or combinations thereof, and where the AbBD is photoreactive. Also provided is an adapter comprising an AbBD that specifically binds and crosslinks to an IgG Fab region and / or to an immunoglobulin M (IgM) Fab region, where the AbBD is a variant of a Protein A domain D and has an amino acid replacement / modification at A28, E50, or L54 of SEQ ID NO: 1 or combinations thereof, where the AbBD is photoreactive. Provided is an adapter comprising an AbBD that specifically binds and crosslinks to an IgG Fab region, where the AbBD is a variant of a Protein L C* domain and has an amino acid replacement / modification at G22 or T46 of SEQ ID NO: 2 or a combination thereof, wherein the AbBD is photo-reactive. Methods for using the AbBD for imaging, diagnosing and / or treating a disease are provided.

The invention discloses a method for identifying glycosylation modification of immunoglobulin M, and belongs to the technical field of protein analysis. The method comprises the following steps: (1) separating IgM from a sample; (2) preparation and enrichment of IgM glycopeptide; and (3) detecting the IgM glycopeptide by mass spectrometry. Two-dimensional electrophoretic separation is carried out on blood / plasma and other samples containing IgM by combining native-PAGE gel electrophoresis and SDS-PAGE gel electrophoresis, the separation method is mild, the glycosylation rate of the obtained IgM is high, and a good foundation is laid for high-coverage identification of subsequent glycosylation modification; by optimizing the enrichment conditions and elution conditions of the modified polydopamine magnetic nanomaterial, high-coverage enrichment of glycopeptides at five N-glycosylation sites of IgM is realized, and the method has great significance in early warning of immune inflammatory diseases, disease progress monitoring, prognosis evaluation and the like.

The invention provides a method for removing IgA (Immunoglobulin A) and IgM (Immunoglobulin M) in human immunoglobulin for intravenous injection. The method comprises the following steps: S1) dissolving plasma component II + III precipitates to obtain a dissolved solution; s2) precipitating and centrifuging the dissolved solution by adopting n-caprylic acid or sodium caprylate to obtain supernate; s3) performing anion exchange column chromatography on the supernate to obtain first flow-through liquid and first washing liquid; s4) mixing the first flow-through liquid with the first washing liquid to obtain a column chromatography collection liquid; s5) performing strong anion membrane chromatography on the column chromatography collection liquid to obtain second flow-through liquid and second washing liquid; s6) mixing the second flow-through liquid and the second washing liquid to obtain a membrane chromatography collection liquid, and S7) sequentially performing first concentration, dialysis, first sterilization, incubation, ultrafiltration and second sterilization on the membrane chromatography collection liquid to obtain the human immunoglobulin for intravenous injection without IgA and IgM. According to the method disclosed by the invention, the IgA and IgM removal efficiency is remarkably improved through a multi-stage purification process.

This invention provides an immunological assay method capable of suppressing nonspecific reactions caused by immunoglobulin M, abbreviated as IgM, contained in a sample for measurement, and reagents used in this immunological assay method. [Solution] The present invention provides an immunological assay method for measuring a target substance in a sample, comprising performing an antigen-antibody reaction at least once in the presence of an enzyme that specifically degrades IgM, and an immunological assay reagent used in the immunological assay method, comprising an enzyme that specifically degrades IgM.

The invention relates to the field of biological detection, in particular to an IgM antibody for preventing red blood cell agglutination and a preparation method and application thereof, and the IgM antibody for preventing red blood cell agglutination is obtained by mixing and incubating a natural IgM antibody and DTT. The IgM antibody can be used for preparing a product for detecting the ABO blood type and a product for detecting the expression quantity of an A-type antigen and / or a B-type antigen, and the prepared product can be used for quantitatively detecting the expression of the ABO antigen, researching the change condition of the expression quantity of different genotypes of ABO antigens, researching diseases related to the expression quantity of the ABO antigens and the like.

A method of preventing or treating or controlling a respiratory disease in a bovine by delivering to a lung or trachea of the bovine a composition including a polyplex having one or more mRNA encoded with a molecule selected from lycosylphosphatidylinositol (GPI) complex, antibody immunoglobulin G (IgG), antibody immunoglobulin A (IgA), antibody immunoglobulin M (IgM), antimicrobial peptide Bactenecin 5 (Bac5), antimicrobial peptide Bactenecin 7 (Bac7), bovine myeloid antimicrobial peptide (BMAP-28), lipoxin inducing enzymes, resolvin inducing enzymes, cytokines, CRISPR associated protein 13 (Cas13) enzymes, nanoluciferase (NLuc) proteins, and gene activator, catalytically dead CRISPR-associated protein 9 fused to an activator selected from the group consisting of VP64-p65-Rta transactivation domain (dCas9-VPR), VP64, and VP64-p65-HSF1 on the N terminus and SS18 on the C-terminus, and a polymer prepared by polymerizing a diacrylate monomer; one or more linker monomers; and one or more branching monomers; and end-capping the polymer.

An immunological measurement method for measuring a measurement target substance in a sample, wherein an antigen-antibody reaction is conducted at least once or more in the presence of an enzyme that specifically degrades Immunoglobulin M, abbreviated as IgM.

The present invention belongs to the field of molecular biological detection technology, and more particularly relates to a molecular marker associated with immune indicators in Tibetan sheep and its application in breeding. The present invention screens and identifies a single-nucleotide polymorphism (SNP) molecular marker associated with immune traits in Tibetan sheep. The SNP marker is located at base 12,065,580 on chromosome 1 of the sheep reference genome Oar_v4.0, with the mutated base being either C or T. This SNP molecular marker can be used to determine the levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M in individual Tibetan sheep, providing a new SNP molecular marker resource for marker-assisted selection of immune traits in Tibetan sheep for non-diagnostic purposes. This provides a basis for breeding Tibetan sheep with high immunity.

The invention belongs to the field of pharmaceutical preparations, and provides a drug sustained-release capsule for treating liver diseases as well as a preparation method and application of the drug sustained-release capsule. The sustained-release capsule provided by the invention is prepared by filling a capsule with sustained-release pellets. The drug carried by the sustained release pellet is IMM-H014 (Immunoglobulin M-H014). The sustained-release pellet is prepared by coating a sustained-release layer on a drug-loaded pellet, and the drug-loaded pellet has the characteristic of delayed release, and is stable in dissolution rate and good in bioavailability. The traditional Chinese medicine composition is used for treating non-alcoholic fatty liver, hepatitis and drug-induced liver diseases.

The invention provides a detection method and a kit for detecting IgM by using a light-activated chemiluminescence double-antigen sandwich method, the purpose of detecting an IgM antibody on a light-activated chemiluminescence platform by using the double-antigen sandwich method is achieved, the problem that the double-antigen sandwich method cannot distinguish IgG and IgM is solved, and the problems that the sensitivity is low and the detection sensitivity is low when the light-activated chemiluminescence indirect method is used for detecting the IgM antibody are solved. The method is easy to be interfered by irrelevant IgM or specific IgG in a sample, needs dilution and is long in time; meanwhile, the problem of interference of specific IgG is solved to a certain extent, and the detection sensitivity and the detection efficiency are improved.

The present invention belongs to the field of molecular biological detection technology, and in particular, relates to the cloning of a SNP molecular marker related to yak immune traits and its application. The present invention obtains through screening that the SNP molecular marker related to yak immune traits is located at the 35789017th base on chromosome 28 of the yak reference genome LU_Bosgru_v3.0, and the mutant base is C or T. The molecular marker can determine the immunoglobulin M content of individual yaks. The present invention provides a new SNP molecular marker resource for marker-assisted selection of yak immune traits for non-diagnostic purposes, and provides a basis for breeding yaks with high immunity.

The invention belongs to the technical field of genetic engineering, and particularly relates to preparation and application of a PRRSV (porcine reproductive and respiratory syndrome virus) specific mouse-pig chimeric IgM antibody. The invention provides two PRRSV (porcine reproductive and respiratory syndrome virus) mouse-pig chimeric IgM (immunoglobulin M) antibodies expressed by genetic engineering recombination, the antibodies comprise a mouse-pig chimeric antibody M1-sIgM and a mouse-pig chimeric antibody M2-sIgM, and the mouse-pig chimeric antibody M1-sIgM has a light chain sequence as shown in SEQ ID NO: 1 and a heavy chain sequence as shown in SEQ ID NO: 2; the mouse and pig chimeric antibody M2-sIgM has a light chain sequence as shown in SEQ ID NO: 5 and a heavy chain sequence as shown in SEQ ID NO: 6. The two antibodies provided by the invention can be used independently or jointly as immunopotentiators of the PRRSV inactivated vaccine to enhance the protective effect of the PRRSV inactivated vaccine.

The present invention relates to an immunological measurement method for measuring a substance to be measured in a sample, the method comprising performing at least one antigen-antibody reaction in the presence of an enzyme that specifically decomposes an immunoglobulin M abbreviated as IgM.

FIELD: occupational medicine.SUBSTANCE: used to diagnose immunocompromise in workers exposed to mixed aerosols containing crystalline silicon dioxide from 10 to 70% and metals in the solid phase. Samples of venous blood and smears from the mucous membrane of the pharynx are taken. In the presence of Streptococcus pneumoniae DNA in scrapings from the mucous membrane of the pharynx and with the level of immunoglobulin M in the blood serum≤ 1.7 g / l is used to diagnose immunocompromise in workers exposed to mixed aerosols containing crystalline silicon dioxide from 10 to 70% and metals in the solid phase.EFFECT: method provides the possibility of selecting immunocompromised workers exposed to industrial mixed aerosols for priority vaccination against pneumococcal infection at the stage of periodic medical examination, by determining the level of immunoglobulin M in the blood serum and Streptococcus pneumoniae DNA in swabs from the mucous membrane of the pharynx.1 cl, 1 tbl, 1 ex

The invention relates to the technical field of biology, in particular to a preparation method of high-content immune globulin rich in secretory immune globulin A. The invention discloses a preparation method of high-content immune globulin rich in secretory immune globulin A. The preparation method comprises the following steps: (1) centrifugally degreasing bovine coloctrum to obtain degreased bovine coloctrum whey; (2) adjusting the pH value of the defatted bovine colostrum whey to 7.0-7.4, and centrifuging to obtain a supernatant; (3) performing ultrafiltration concentration and freeze drying on the supernate to obtain immune globulin; according to the immune globulin prepared by the method, the SIgA content in the immune globulin is 8-15%, and the total content of secretory immune globulin A, immune globulin M and immune globulin G in the immune globulin is greater than 60%; the preparation method provided by the invention has no complex pretreatment step, and is a simple, rapid and efficient method for preparing high-content immune globulin rich in secretory immune globulin A.

The present application relates to methods of treating infectious diseases with compositions containing plasma-derived immunoglobulin M (IgM). The compositions and methods of the invention prevent, inhibit, or reduce the toxic effects of proteins and toxins secreted by microorganisms. A method for neutralizing a microbial protein product in a subject includes administering to the subject a composition comprising plasma-derived IgM and, optionally, one or more excipients in the form of a pharmaceutical carrier, wherein the composition is administered in an amount effective to neutralize the microbial protein product.

The present invention belongs to the technical field of molecular biological detection, and particularly relates to a molecular marker associated with a yak immune index and the use thereof in breeding. In the present invention, an SNP molecular marker associated with a yak immune trait is obtained by means of screening. The marker is located at base position 26593056 on chromosome 3 of the yak reference genome assembly LU_Bosgru_v3.0, with the mutant base being G or A. In the present invention, the contents of immunoglobulin A, immunoglobulin G and immunoglobulin M in a yak individual can be obtained by using the SNP molecular marker, which provides a new SNP molecular marker resource for marker-assisted selection of yak immune traits for non-diagnostic purposes, thereby providing a basis for the breeding of yaks with high immunity.

The present application relates to the technical field of molecular biology detection, and provides a SNP molecular marker affecting yak immunoglobulin and application of the SNP molecular marker. The present application obtains, through screening, that the SNP molecular marker related to the yak immunity trait is located at the 43165308th base on the 21st chromosome of the yak reference genome LU_Bosgru_v3.0, and the mutation base is C or T. The molecular marker can determine the contents of immunoglobulin A and immunoglobulin M of a yak individual. The present application provides a new SNP molecular marker resource for marker-assisted selection of non-diagnostic yak immunity traits, and provides a basis for improving the breeding of yak immunity.