118 results about "Hand foot mouth disease" patented technology

The invention relates to valerolactam enterovirus 71 (EV71) 3C protease inhibitors, wherein a structure general formula of the inhibitors is represented by a compound M, various variables in the structure is defined in an instruction, and EV71 replication is effectively inhibited or blocked with the compounds. The present invention relates to discoveries and applications of the compounds containing the structure represented by the formula (M), optical isomers, metabolites with pharmaceutical activities, pharmaceutically acceptable salts, solvates, and prodrugs thereof in preparations of anti-virus drugs for treatment of hand-foot-mouth disease infection. The present invention further relates to an intermediate of the structure compound represented by the formula (M) and a synthesis method thereof.

A deep learning based hand-foot-and-mouth disease detection system comprises a neural network model training module and a hand-foot-and-mouth disease detection module. A convolutional neural network model is constructed through the neural network model training module on the basis of a hand-foot-and-mouth disease sample set, and a hand-foot-and-mouth disease neural network diagnosis model is obtain by analysis of images in the hand-foot-and-mouth disease sample set. On the basis of input images, the hand-foot-and-mouth disease detection module judges to obtain hand-foot-and-mouth disease diagnosis results through the neural network diagnosis model. By application of the deep learning technology to automatic diagnosis of the hand-foot-and-mouth disease, a hand-foot-and-mouth disease detection core problem is transformed into a target detection problem, and detection result accuracy is improved by autonomous optimization.

The invention discloses application of Chinese medicinal composition in preparation of medicament for treating hand-foot-and-mouth disease. The Chinese medicinal composition comprises weeping forsythiae capsule, honeysuckle flower, ephedra herb, bitter apricot seed and the like. The Chinese medicinal composition has the function of broad-spectrum anti-virus, and can effectively treat hand-food-and-mouth disease by effectively killing virus, eclipsing fever and diminishing inflammation.

The invention provides an environmental health risk monitoring and warning system and method based on a spatial Bayesian network. Spatial correlation and spatial aggregation factors are added on the basis of the traditional Bayesian network, optimized machine learning is closely combined with domain knowledge, and therefore, a network model with a more reasonable structure is generated. A spatialcorrelation / aggregation inference link is added on the basis of existing factors, and thus, the ability of Bayesian network environmental health risk monitoring and warning is enhanced. The hand-foot-mouth disease is affected by many environmental factors, which is taken as an example in the invention to explain the principle of the spatial Bayesian network and the advantage of the spatial Bayesian network in environmental health risk assessment and warning. The system and the method of the invention can be applied to the risk monitoring and warning of various diseases or health problems closely related to environmental health.

The invention discloses a univalent and bivalent gene engineered subunit vaccine for preventing Enterovirus 71 (EV71) and Coxsackie virus A16 (Cox.A16) of hand-foot-and-mouth disease, and a preparation method thereof. The preparation method comprises the following steps: respectively obtaining recombinant baculovirus Bac-EV71-P1-3CD and Bac-Cox.A16-P1-3CD by gene engineering means, respectively efficiently coexpressing similar SeQ ID No.1 EV71 P1 and Se Q ID No.2 Cox.A16 P1 and 3CD proteins in insect cells, and respectively self-assembling into EV71 VLP and Cox.A16 VLP; establishing and verifying a vaccine strain third-stage seed lot library; culturing cells; inoculating and propagating virus; lysing the cells, ultra-filtering and purifying virus suspension; and further preparing the univalent and bivalent vaccine. The vaccine has good application prospect for preventing the hand-foot-and-mouth disease.

The invention relates to an enterovirus type 71 (abbreviated as EV71) and use thereof, in particular to a monoclonal virus strain which is separated by a plaque assay method. The progeny viruses of the monoclonal virus strain show genetic stability. The monoclonal virus strain can be used in the production of a vaccine (particularly the production of a vaccine for infant). The virus strain or the vaccine produced by using the same can be used in the prevention of diseases (such as hand-foot-and-mouth diseases, particularly hand-foot-and-mouth diseases in children) caused by the EV71 and has the characteristics of stable titer, high immunogenicity and small immunizing dose.

The invention provides an agarose compatible medium for purifying immune globulin of the hand-foot-and-mouth disease and a preparation method thereof. In the agarose compatible medium, agarose gel is used as a matrix, is activated by an epoxy activating agent, and is coupled with vaccine of the hand-foot-and-mouth disease which is used as petunidin, wherein the agarose gel is made of one of agarose 6FF, agarose 4FF, agarose CL-6B, agarose CL-4B, agarose 6B and agarose 4B. The agarose compatible medium can be used for purifying the immune globulin of the hand-foot-and-mouth disease, has the advantages of good medium selectivity, strong specificity, strong mechanical strength and industrial amplification benefit. Furthermore, the preparation process is simple and is easy to amplify. The medium prepared by the method is adopted for purifying the immune globulin of the hand-foot-and-mouth disease, and has simple and feasible purification method and good separation effect.

The present invention relates to viral particles as immunogens against enterovirus infection and a method of producing the same. Specifically, the present invention features that human embryo kidney 293 (HEK 293) cells are used to produce viral particles of Enterovirus A, particularly Coxsackievirus A6 (CVA6) particles or Coxsackievirus A10 (CVA10) particles or both and optionally additional viral particles of other Enterovirus A e.g. Coxsackievirus A16 (CVA16) and / or Enterovirus A71 (EV71). The yield of the viral particles in HEK 293 cells is unexpectedly high and effective to induce an immune response against enterovirus infection, especially CVA6 and CVA10. The present invention also relates to an immunogenic composition against enterovirus infection for human use comprising the viral particles as described herein and a method of preventing enterovirus infection or a disease as caused, particularly Hand-Foot-Mouth diseases (HFMD), by administering the immunogenic composition to a subject in need thereof.

The invention relates to the pharmacy field, concretely relates to a use of a series of pentacyclic triterpene compounds as enterovirus EV71 inhibitors, and especially relates to an application in the preparation of medicines for preventing and treating EV71 infection induced hand-foot-and-mouth diseases and complications thereof, such as synanche, myocarditis, pulmonary edema, encephalitis, herpes, septicemia, hypertension, hyperglycemia, cognitive function disorder, poliomyelitis-like paralysis and many nerve system associated diseases. The invention also discloses medicinal compositions of the series of the pentacyclic triterpene compounds as enterovirus EV71 inhibitors.

The invention relates to a double-vanilla preparation, which is a medicament prepared by the following raw materials in part by weight: 50-600 parts of white honeysuckle flower, 50-600 parts of weeping forsythia, 50-600 parts of wrinkled gianthyssop herb, 50-300 parts of peppermint, 50-600 parts of lithospermum, 50-300 parts of wormwood leaf and 50-300 parts of raw licorice. The invention also provides a preparation method of the double-vanilla preparation, which comprises the following steps: boiling and distilling the white honeysuckle flower, weeping forsythia and the like in water for 1-20 times and for 0.5-5 hours and simultaneously collecting essential oil for standby, wherein water is added for soaking for 0-2 hours before boiling and distilling, the quantity of water is 5-20 times of the weight of the seven kinds of traditional Chinese medicines; filtering the boiled liquid until the relative concentration of the filtrate is 0.5-2; adding ethanol in the filtrate, standing and filtering; recovering ethanol until the filtrate is tasteless; adjusting a pH (Potential of Hydrogen) value: adjusting the pH value of the filtrate obtained by recovering the ethanol to 3-7; and diluting, filtering and disinfecting. The invention has good pesticide effect to upper respiratory tract infection, remarkable treatment effects, quicker fever abatement of patients with a hand-foot-mouth disease and deflorescence and good medicine properties; and as the atomization preparation is adopted, the medicine can be conveniently used, and compliance of patients is good.

The invention discloses a pharmaceutical composition for treating an infantile hand-foot-and-mouth disease. The pharmaceutical composition comprises the following raw materials in parts by weight: 30-80 parts of radix bupleuri, 20-60 parts of scutellaria baicalensis, 30-80 parts of raw gypsum, 60-100 parts of honeysuckle, 20-50 parts of fructus forsythiae, 30-80 parts of radix isatidis, 30-80 parts of radix puerariae, 20-50 parts of radix scrophulariae, 20-50 parts of rhizoma phragmitis, 10-30 parts of fried almond and 10-30 parts of raw liquorice. An oral preparation prepared by using a conventional method for a Chinese material medical preparation is used for treating the infantile hand-foot-and-mouth disease, and is accurate in curative effect and free of side effects.

The invention discloses a colloidal gold test strip for rapid detection of Human enterovirus 71 (EV71) IgM (immunoglobulin m), belonging to the field of medical detection consumables. The colloidal gold test strip is characterized in that a colloidal gold-labeled cellulose pad is coated with colloidal gold-labeled EV71 antibody; a control line C is coated with anti-mouse IgG polyclonal antibody; a test line T is coated with anti-Mu chain monoclonal antibody; and a sample cellulose pad is coated with EV71 recombinant antigen. The inventive test strip adopts antibody capture principle and colloidal gold technology to detect EV71 IgM in serum / plasma / whole blood samples simply, sensitively, precisely and rapidly; and can be used for the rapid detection of EV71 IgM and diagnosis of hand-foot-mouth disease.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a water extract of houttuynia cordata thunb and a preparation method and an application of the water extract. Total polysaccharide obtained from the water extract of the houttuynia cordata thunb has activity to inactivate or inhibit replication of hand-foot-mouth disease viruses, namely an enterovirus-71 (EV-71) and coxsackie A-16 (CVA16); polysaccharide with the antiviral activity is further found to be polysaccharide with molecular weight greater than 100kDa; therefore, the polysaccharide can be applied in preparation of a medicine for preventing the hand-foot-and-mouth disease viruses. As an edible aquatic herbaceous plant, the houttuynia cordata thunb has the characteristics of extensive source, small toxic and side effects, low price, simple extraction process and the like, and has important development and application prospects.

The invention discloses a composition used for detecting enterovirus causing hand foot and mouth disease, which comprises an upstream primer P1:5'-CCCTGAATGCGGCTAATCC-3' and a downstream primer P2:5'-GTCGGTTCCGCTGCAGAGT-3'. Genetic fragments of 5'UTR regions of EV71 and Cox A16 type enteroviruses in a sample can be specifically detected by the composition matched with a proper probe and other reagents through a real time Polymerase Chain Reaction (PCR) method. The invention also discloses a kit containing the composition, and a method for detecting the EV71 and / or Cox A16 type enterovirus by utilizing the composition or the kit. When the composition or the kit and / or the method is utilized for detecting the sample, whether the EV71 and / or Cox A16 type enterovirus exist in the sample can be sensitively and rapidly detected in a reaction; therefore, the efficiency and the sensitivity of the detection are improved, the detection process is simplified, and the manpower, the material resources and the detection cost are saved.

The invention relates to a new drug using present antibiosis and antiphlogosis drug andrographolide and its derivative to prevent and treat the hand-foot-and-mouth disease, and provides a new uses of andrographolide drugs. The invention concretely relates to a use of the andrographolide drugs in the preparation of drugs for preventing and / or treating hand-foot-and-mouth disease virus infection. Pharmacodynamics researches on the andrographolide drugs, such as andrographolide, neoandrographolide, sulfonated andrographolide, triacetyl andrographolide and dehydroandrograpolide succinate show that andrographolide and its derivative are high efficiency drugs for preventing and treating the hand-foot-and-mouth disease, can be processed to prepare various common dosage forms used for clinical prevention and treatment, and have the characteristics of good curative effect, low toxicity and low price. A new drug is provided for the prevention and treatment of clinic hand-foot-and-mouth disease patients, and important bases are provided for finding a new theory and a new drug action target.

The invention relates to a primer for coxsackie virus A16 nucleic acid detection, probe and kit, wherein the nucleotide sequence of the primer is that: forward primer 5'-GAACCATCACTCCACACAGGAG-3'; backward primer 5'-GTACCCGTGGTGGGCATTG-3' and the nucleotide sequence of the probe is 5'-CAGCCATTGGGAATTTCTTTAGCCGTG-3'. The invention also provides a method for detecting coxsackie virus A16 nucleic acid. Sample RNA is used as template and the above primer and probe are subjected to real-time fluorescence RT-PCR amplification and the result is predicated based on the amplification curve after each circulation. The primer specificity is good, the detection method is quick and simple, the accuracy and the sensitivity is high and the invention provides scientific reference for etiologic diagnosis and differential diagnosis of hand-foot-mouth disease and relative diseases.

The invention relates to new application of luteolin, in particular to application of luteolin in preparing medicine for preventing and treating hand-foot-mouth diseases, particularly, medicine used for resisting enterovirus 71 and / or coxsackievirus A16.

The invention provides a hand-foot-and-mouth disease prediction method and device using fine-grained data, electronic equipment and a computer readable medium. The method comprises the steps of obtaining historical case data of the hand-foot-and-mouth disease; preprocessing the historical case data, and dividing the historical case data into time series data of two different time intervals; pre-aggregating the time series data of the two different time intervals according to time to obtain multivariate time series data, and converting the multivariate time series data into supervised data; training a hand-foot-and-mouth disease prediction model according to the supervised data; inputting the hand-foot-and-mouth disease case data collected in real time into the trained hand-foot-and-mouth disease prediction model to obtain the real-time predicted number of patients with the hand-foot-and-mouth disease. According to the scheme, the time series data of different granularity time intervalsof historical case data are obtained, the accuracy of predicting the number of patients with the hand-foot-and-mouth disease based on equal time granularity is improved according to the time series data with finer granularity, additional data assistance is not needed, and the prediction efficiency is improved.

The present invention relates to a new use of a small molecule inhibitor, and specifically to a use of a CDK small molecule selective inhibitor 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine or a pharmaceutically-acceptable salt thereof in preparation of drugs for treatment of hand-foot-mouth disease virus infection-related diseases caused by EV71 and the like.

The invention discloses a kindergarten morning check method which comprises the following steps: firstly, creating a neuron model, and then training the neuron model through a large number of herpes,hand-foot-mouth disease, angina and pinkeye pictures; collecting image information of the hands and the oral cavity of the to-be-detected person through an image collection unit; inputting the image information acquired by the image acquisition unit into a neuron model for detection, and finally outputting a result. According to the method, the mouth, the hands and the face of the infant are shotthrough the camera and are then discriminated and calculated through trained neurons. The whole detection process is fast, a computer system does not feel tired, and the accuracy can be guaranteed aslong as early-stage neurons are trained. In addition, the invention also discloses a kindergarten morning check device which comprises an image acquisition unit, an image processing module, a neuron model module and an output module.

The invention provides human anti-EV71 (enterovirus 71) neutralizing antibody EV71FabK7 which is obtained by screening by means of phage display. Amino acid sequences of light chain and heavy chain variable areas are shown as SEQ ID No.1 and SEQ ID No.2 respectively. The antibody can specifically recognize EV71 virus particle antigens, can have evident enzyme-linked immune reaction with EV71 viruses, and has a function of neutralizing activity resistant to EV71 virus infection. Further, the antibody can be made into specific antibody medicine for preventing and treating the hand-foot-mouth disease and clinically used for preventing and treating the hand-foot-mouth disease caused by EV71 viruses.

The invention discloses a traditional Chinese medicine solid preparation used for preventing hand-foot-mouth diseases and exopathic diseases and treating mild symptoms, wherein drug substances of the traditional Chinese medicine solid preparation adopt the recipe as follows: 250 to 350 parts of honeysuckle, 250 to 350 parts of, forsythia,, 160 to 240 parts of ageratum, 70 to 130 parts of artemisia leaves, 70 to 130 parts of menthol, 160 to 240 parts of puccoon, and 70 to 130 parts of raw glycyrrhiza .

The invention provides a preparation method and application of a recombinant vaccine host protein antibody, an expression vector which is used for preparing a recombinant vaccine and does not carry any exogenous gene is introduced into prokaryotic or eukaryotic host cells completely same as that used for preparing the recombinant vaccine to construct a transformant, the transformant is cultured and expanded, and extracted and purified by a method completely same as that used for preparing the recombinant vaccine to obtain a recombinant vaccine host protein; and the recombinant vaccine host protein antibody is obtained by animal immunization and antibody purification. The antibody provided by the invention can be applied to detection of host protein residues in recombinant hand-foot-mouth disease vaccines, is high in sensitivity, good in repeatability, and good in specificity, and is suitable for detection of the host protein residues of a biological product expressed by hansenula polymorpha, so that the application prospect is very wide.

The invention belongs to the field of traditional Chinese medicines, and particularly provides a callicarpa nudiflora extract composition for treating a hand-foot-and-mouth disease. According to the parts by weight, the composition is prepared by 22-40 parts of total callicarpa nudiflora flavone, 20-26 parts of total callicarpa nudiflora phenolic acid, and 40-50 parts of total callicarpa nudifloraphenylethanoid glycosides. The invention further discloses an application of the composition. Three components used by the composition are extracted from callicarpa nudiflora, and the components of the composition are clear. Through the synergistic effect of each component, the composition has the good efficacy of treating and / or preventing the hand-foot-and-mouth disease.

The embodiment of the invention discloses a hand-foot-and-mouth disease detection method, device and system based on thermal infrared and RGB double shooting. The hand-foot-and-mouth disease detectionmethod comprises the following steps: acquiring temperature through a thermal infrared camera; acquiring face and hand images through an RGB camera; performing spatial association on the temperatureand the human face to obtain an association result; using an improved YOLO-V3 focus detection network to detect the face and hand images; combining the temperature, the association result, the face detection result and the hand detection result to carry out joint judgment to obtain a judgment result; and triggering whether to alarm according to a judgment result. For the hand-foot-and-mouth disease detection method, body temperature measurement and hand-foot-and-mouth detection can be completed at the same time; all detection and calculation show a detection result within 1s at a local end with a neural network accelerator; whether the body temperature and the skin have papules, macular papules or herpes lesions or not is judged in a combined mode; the hand-foot-and-mouth disease detectionaccuracy is improved; an abnormal alarm is further set; and a worker can be reminded to treat a detected person in time.

The invention relates to a nursing combination device for child hand foot mouth disease. First, clothes are hanged on a bracket. The bracket can be located in a sterilizing hood. A UV disinfection lamp and a drying lamp are turned on. Then a first drive motor is turned on. When a driving rod rotates, an active rotating disk is driven to rotate. When the active rotating disk rotates, a driven rotating disk is driven to rotate intermittently by a driving column. When the driven rotating disk rotates, the bracket is driven to rotate intermittently. When the UV disinfection lamp and the drying lamp rotate to the bracket, the clothes can be sterilized or dried continuously from different angles. Therefore, the purposes of rapid disinfection and drying can be achieved, the disinfection effect isgood, and working efficiency is high. The nursing and isolation of sick child can be achieved through a baby care bed.

The invention discloses an external traditional Chinese medicine lotion for treating hand-foot-mouth disease rash. The hand-foot-mouth disease is a viral skin disease which is characterized by herpes at hands, feet and mouth and always seen to children. The external traditional Chinese medicine lotion is prepared from the traditional Chinese medicines such as folium agaves america nae, folium styracis dasyanthi, denseflower bulbophyllum herb, nagens groundsel, peucedanum terebinthaceum, trema cannabina, erythrina arborescens, lecomte bauhinia leaf or root, siberian columbine meadowrue herb and astragalus bhotanensis with the effects of releasing lungs, clearing heat, dispelling dampness and detoxifying via decoction with water; clinic experiments prove that the total effectiveness is 98.44% and superior to the control group, and the deflorescence time is shorter than the control group, so that the external traditional Chinese medicine lotion is worthy of clinic popularization and application.

The invention discloses a combined nursing device for an infantile hand-foot-mouth disease. The combined nursing device for the infantile hand-foot-mouth disease mainly comprises a nursing cabinet part and a nursing bed part. The nursing cabinet part comprises an ultrasonic atomizer, a physiological monitor, a disinfection cabinet, an air purifier and an oxygen tank. The ultrasonic atomizer is located at the upper left of the nursing cabinet part, and the physiological monitor is located on the right side of the ultrasonic atomizer, the disinfection cabinet is located below the ultrasonic atomizer, the air purifier is located on the left side of the disinfection cabinet, and the tail end of the nursing bed is fixedly connected with the lower end of the nursing cabinet part. In a word, thecombined nursing device for the infantile hand-foot-mouth disease integrates all kinds of devices needed for the nursing of the infantile hand-foot-mouth disease, the design is reasonable, the operation is convenient, the working efficiency is greatly improved, and the workload of the nursing staff is reduced.

The invention discloses a suite reagent for titrating the titer of an EV71IgM antibody and an EV71IgG antibody of a hand-foot-mouth disease patient, comprising an ELISA (Enzyme Linked Immuno Sorbent Assay) plate and a detection reagent. The ELISA plate of the EV71IgM antibody comprises a polystyrene board, 1gM capture antibody is coated in polystyrene board holes, the ELISA plate of the EV71IgG antibody comprises a polystyrene board, and 1gM capture antibody is coated in the polystyrene board holes, and the detection reagent is EV71VP1 labeled by HRP (Horseradish Peroxidase). The suite reagent of the invention can be used for detecting the antibody titer of the hand-foot-mouth disease patient, the detection result can be used as a judgment result for judging disease outcome so that a clinical severe case can be predicted, prevented and controlled to lessen the probability of death cases.

The invention discloses a traditional Chinese medicinal liquid preparation suitable for preventing and treating the hand-foot-and-mouth disease and external infection diseases. The traditional Chinese medicinal preparation is prepared by carrying out a scientific processing technology on honeysuckle flower, Weeping Forsythia, Wrinkled Gianthyssop, tarragon, mint, Lithospermum erythrorhizon and raw licorice. The traditional Chinese medicinal preparation for preventing and treating the hand-foot-and-mouth disease and the external infection diseases caused by the exogenous evil utilizes the traditional Chinese medicinal theory and is clinically demonstrated. The preparation of the invention, which has a traditional Chinese medicinal preparation formula, has obvious inhibition effects on staphylococcus aurei, hemolytic streptococcocci, dysentery, typhoid, diplococcus meningitides, diplococcus pneumoniae, green plus, influenza viruses and the like.