50 results about "Igm antibody" patented technology

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The invention discloses mannheimia haemolytica outer membrane vesicles as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The OMV is separated from Mh-5 strain culture supernatant through tangential flow ultrafiltration combined with an ultracentrifugation technology, and animal experiments prove that a high-level IgG / IgM antibody can be induced by intramuscular injection of 50 micrograms per mouse, expression of inflammatory factors such as IL-6 and TNF-alpha of the spleen is remarkably improved, the protection rate after challenge reaches 85.7%, and pathological damage is remarkably lighter than that of an inactivated vaccine group. According to the scheme provided by the invention, the problems of insufficient serotype coverage and antibiotic resistance in the prior art are solved, and a new strategy is provided for prevention and control of mannheimia haemolytica.

The invention provides a joint detection test strip for dengue virus and Zika virus antigens and antibodies and a preparation method of the joint detection test strip. According to the combined detection test strip, the combined detection test strip with the dengue virus NS1 antigen and the dengue virus IgG / IgM antibody adopts a multi-site recombinant antigen labeling or multi-site recombinant antibody coating form, so that the sensitivity of a reagent is improved; the problems of poor specificity and release residue of latex microspheres are solved by optimizing the confining liquid; colored latex microspheres are used as a marker, a detection target is more intuitively distinguished according to line colors, clinical result interpretation is facilitated, the accuracy of dengue virus infection and Zika virus infection is more quickly improved, and the dengue virus NS1, the Zika virus NS1 antigen, the dengue virus IgG / IgM antibody and the Zika virus IgG / IgM antibody can be detected at the same time; and the kit is convenient to carry, simple to operate, convenient for on-site instant detection and capable of performing large-scale preliminary screening.

A composition and method for therapeutic or prophylactic treatment of mammals includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum. In some embodiments the composition can also include IgA and IgM antibodies.

The invention discloses an early and late latent syphilis prediction model based on a treponema pallidum nonspecific IgM antibody, and relates to the technical field of biology. The objective of the invention is to solve the problem of inaccurate latent syphilis staging caused by dependence on subjective recall and single index in the prior art. The prediction model is a hume diagram model and comprises three indexes, namely a treponema pallidum non-specific IgM antibody, a treponema pallidum specific antibody and a treponema pallidum non-specific antibody. The method provides a basis for timely and accurately performing medical intervention on early and late latent syphilis patients, and has an important clinical application value.

The present invention relates to the technical field of antibody storage, and specifically to an anti-contamination test kit for detecting IgM antibodies and a sealing method thereof, comprising: a test kit body, on which a cover plate is rotatably mounted; a loading assembly, which comprises a No. 1 support plate and a No. 2 support plate; a lifting assembly, which can drive the No. 1 support plate and the No. 2 support plate to move toward the upper part of the test kit body when the cover plate is opened; a pulling assembly, which is arranged between the No. 1 support plate and the No. 2 support plate, and can move the No. 2 support plate toward the No. 1 support plate when the No. 1 support plate rises to a high point of its travel; a clamping assembly, which is arranged between the No. 1 support plate and the test kit body, and is connected to an arc-shaped rubber piece, and can drive the arc-shaped rubber piece to abut against a test tube inserted between the No. 1 support plate and the No. 2 support plate when the No. 1 support plate descends to a low point of its travel, so as to prevent the test tube from shaking in the test kit and breaking.

The application provides a combined detection test strip for dengue virus, Zika virus antigen and antibody and a preparation method thereof; the combined detection test strip of the application adopts a multi-site recombinant antigen marking or multi-site recombinant antibody coating form for the combined detection test strip for dengue virus NS1 antigen and dengue virus IgG / IgM antibody, thereby improving the sensitivity of the reagent; the application also overcomes the problems of poor specificity and release residue of the latex microspheres by optimizing a blocking solution; color latex microspheres are used as the markers, the target is more intuitively detected according to the color of the lines, clinical result interpretation is facilitated, the accuracy of dengue virus infection and Zika virus infection is improved, dengue virus NS1, Zika virus NS1 antigen, dengue virus IgG / IgM antibody detection and Zika virus IgG / IgM antibody can be simultaneously detected; moreover, the application is convenient to carry and easy to operate, is convenient for on-site instant detection and can be used for large-scale preliminary screening.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

The present invention relates to an IgM antibody formulation and uses thereof. In particular, the IgM antibody formulation comprises an IgM antibody, which is an IgM antibody formed by a nanobody fusion protein and a J chain, the nanobody fusion protein is a fusion protein comprising a nanobody and an Fc fragment, which are optionally linked by a linker, wherein the formulation further comprises a buffer selected from a citrate buffer system, optionally the formulation further comprises one or more of a stabilizer, an osmotic pressure regulator and a surfactant.

An immunological analysis mode of a novel total antibody assay and a kit for detecting antibodies of infectious diseases in human blood samples according to the mode, the kit comprising: a first reagent containing at least one antigen coated on a solid support and an anti-human IgM antibody coated on a solid support; and a second reagent containing at least one labeled antigen and a labeled anti-human IgG antibody; wherein at least one antigen of the at least one antigen coated on the solid support and at least one antigen of the at least one labeled antigen can bind to the same IgG antibody or the same IgM antibody in the sample. The kit can overcome the disadvantages caused by the detection principle while retaining the advantages of each detection principle. In addition, a method for detecting antibodies produced after pathogen infection in a sample is provided.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of mumps virus, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of the IgG monoclonal antibody which is specifically combined with the mumps virus, and the IgG monoclonal antibody is of a mouse source or a rabbit source. According to the present invention, the IgG antibody is modified to obtain the recombinant IgM antibody, and the IgG antibody and the recombinant IgM antibody are not only suitable for the chemiluminescence method, but also show the expected sensitivity amplification factor in other immunological detection methods, such that the low-abundance detection of the specifically bound mumps virus can be easily achieved.

The invention discloses a non-human animal integrated with a double-derived VH subclass fusion gene as well as a construction method and application of the non-human animal. The method comprises the following steps: firstly, constructing a gene construct containing human IGHV1-69 and IGHV3-23 fragments, a specific spacer region, a functional element and a rat heavy chain 3'enhancer, and microinjecting the gene construct into a C57BL / 6J fertilized egg with an endogenous heavy chain and a kappa light chain knocked out to obtain a transgenic mouse; through identification, after a mouse endogenous antibody is inactivated, a human gene is stably integrated, a human IgM antibody is expressed and H5N1 HA immunization is carried out, the use frequency of IGHV1-69 is remarkably increased, the mouse produces a high-titer specific antibody, and serum has neutralizing activity. The mouse can generate a target bnAbs aiming at the HA stem and the RBS in parallel, so that the antibody screening efficiency is improved, and an in-vivo screening and evaluation platform for simulating human body fluid response is provided for research and development of influenza antibodies and vaccines.

Provided herein are antibodies, such as IgM antibodies, that bind to human PSGL-1. These antibodies can be used in a variety of diagnostic and therapeutic methods, including, without limitation, treating T-cell mediated inflammatory diseases, cancers, transplantations, and transfusions.

The present invention provides an anti-mutant citrullinated vimentin antibody detection kit and its application, which relates to the field of biotechnology. The kit includes a mutant citrullinated vimentin-coupled magnetic particle working solution, which is mixed with a final feed ratio of 0.6 ng / (unit × mL) mutant citrullinated vimentin polypeptide E1-coupled magnetic particles, 0.3 ng / (unit × mL) mutant citrullinated vimentin polypeptide H1-coupled magnetic particles, 0.3 ng / (unit × mL) mutant citrullinated vimentin polypeptide G5-coupled magnetic particles and a buffer; a tracer-labeled antibody working solution, which is composed of a tracer-labeled anti-human IgM antibody and a diluent. The kit of the present invention uses a mixture of mutant citrullinated vimentin polypeptide E1-coupled magnetic particles, mutant citrullinated vimentin polypeptide H1-coupled magnetic particles, and mutant citrullinated vimentin polypeptide G5-coupled magnetic particles to greatly improve sensitivity and specificity.

The invention relates to the technical field of recombinant antibody expression, in particular to a molecular chaperone PDI for improving IgM recombinant expression efficiency and application of the molecular chaperone PDI, and the molecular chaperone PDI comprises an amino acid sequence as shown in SEQ ID NO: 3. By means of molecular chaperone co-expression, the expression stability of IgM can be remarkably improved, meanwhile, the flow detection signal intensity of the recombinant expression IgM antibody is equivalent to that of a commercial IgM antibody from hybridoma, the expression condition of the recombinant antibody is stable, the batch-to-batch difference in the batch production process is small, and the production cost is low. The recombinant expression can better avoid the risk of antibody gene sequence loss (such as non-expression) generated in the continuous production process of the hybridoma, and also can avoid possible variation (such as glycosylation difference) of antibodies secreted in different batches, so that an optimization strategy is provided for recombinant expression of IgM.

This invention relates to the field of biological detection, and in particular to an IgM antibody for preventing erythrocyte agglutination, its preparation method, and its uses. The IgM antibody for preventing erythrocyte agglutination is obtained by mixing and incubating a natural IgM antibody with DTT. The IgM antibody can be used to prepare products for detecting ABO blood types and products for detecting the expression levels of type A antigen and / or type B antigen. The prepared products can be used for quantitative detection of ABO antigen expression, studying changes in ABO antigen expression levels among different genotypes, and studying diseases related to ABO antigen expression levels.

The invention discloses a detection test strip and a detection card for detecting a coxsackie virus group B IgM antibody as well as a preparation method and application of the detection test strip and the detection card. The detection test strip comprises a bottom plate as well as a nitrocellulose membrane, absorbent paper, a conjugate pad and a sample pad which are sequentially overlapped and fixed on the bottom plate, the nitrocellulose membrane is coated with a detection line and a quality control line, the detection line is coated with an antigen of an anti-coxsackie virus group B IgM antibody, and the quality control line is coated with an IgG polyclonal antibody; the conjugate pad comprises an antigen conjugate of a colloidal gold-anti-coxsackie virus group B IgM (Immunoglobulin M) antibody; the particle size of the colloidal gold is larger than or equal to 100 nm. The detection test strip prepared by the invention is high in sensitivity, good in repeatability and strong in specificity, and can be used for detecting the coxsackie virus group B IgM antibody. The device is convenient to use, easy to operate, short in consumed time and convenient to carry.

The blocking agent provided by the invention comprises a human IgM antibody, and the antibody has good binding reaction activity on human IgM, has good blocking and immune interference eliminating effects when being used for immunodetection, and provides an important blocking agent raw material source for immunodetection.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of HSV-II, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of the IgG monoclonal antibody specifically combined with the herpes simplex virus HSV-II, and the IgG monoclonal antibody is of a mouse source or a rabbit source. According to the present invention, the IgG antibody is modified to obtain the recombinant IgM antibody, and the IgG antibody and the recombinant IgM antibody not only are suitable for the chemiluminescence method, but also show the expected sensitivity amplification factor in other immunological detection methods, such that the low-abundance detection of the specific binding herpes simplex virus HSV-II can be easily achieved.

The invention discloses a pathogenic microorganism infection IgM antibody hypersensitivity detection method based on PEG mixed sensitization-three-stage gradient washing-ALP double activation collaborative optimization and a kit. The method is characterized in that PEG 6000 and PEG 20000 are matched according to a ratio of 4: 1 to synergistically enhance sensitivity and strengthen antigen-IgM binding; three-stage gradient washing decoupling background reduction and enzyme activity keeping contradiction; an ALP double-activation system (Mg < 2 + > / Zn < 2 + > + 5-(myristyl amino) fluorescein + Gemini) improves the enzyme activity to be greater than or equal to 320U / mg. The kit contains six reagents such as PEG-biotin labeled antigens, the detection limit is smaller than or equal to 0.5 pg / mL (1000 times higher than that of a traditional reagent), the background is smaller than or equal to 350RLU, the cross reaction rate is smaller than or equal to 0.2%, the detection rate within 3 days of disease attack is larger than or equal to 90%, and the activity attenuation is smaller than or equal to 8% after the kit is stored at 4 DEG C for 12 months. The replacement of specific antigens can be expanded to detection of other pathogens / tumor markers.

The present application relates to the technical field of biology, and in particular to an enzyme conjugate diluent and detection reagent for detecting IgM antibody of Legionella pneumophila. The enzyme conjugate diluent comprises 0.01-0.1M buffer, 2-5M chloride salt and the following components with mass volume percentage: 0.5-5% protective agent, 0.01-0.05% CaCl2, 0.1-0.5% preservative and 0.01-0.05% pigment; the chloride salt is NaCl and / or KCl. The enzyme conjugate diluent provided by the present application effectively enhances the antigen-antibody reaction rate by adding chloride salt with appropriate concentration, so that the detection value reaches the saturation state, the influence of random error and systematic error is reduced, thereby effectively improving the precision of detection, and the detection time is shortened.

The application discloses an anti-IgM antibody and application thereof, and relates to the field of antibodies.The anti-human IgM antibody provided by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, has a good blocking and immune interference eliminating effect, and provides an important blocking agent raw material.

The invention discloses a respiratory pathogen IgM antibody detection kit and a detection method. The respiratory pathogen IgM antibody detection kit comprises a pathogen antigen membrane chip, a sample diluent, a fluorescein conjugate M and a washing solution, wherein the sample diluent, the fluorescein conjugate M and the washing solution respectively contain a 4-(2-ethoxyl) piperazine-1-ethanesulfonic acid buffer solution. The respiratory tract pathogen IgM antibody detection kit provided by the invention has excellent sensitivity and specificity on respiratory tract pathogen IgM antibody detection, and has good stability. Moreover, the kit disclosed by the invention combines a high-specificity immunoreaction and a high-sensitivity fluorescence excitation determination technology, so that the reaction speed is high, the detection sensitivity is high, and the linear range is wide.

The invention discloses a riemerella anatipestifer glucosamine oligosaccharide protein conjugate as well as a preparation method and application thereof, the glucosamine oligosaccharide protein conjugate is formed by connecting glucosamine oligosaccharide and carrier protein through a linking group, and the structural general formula of the glucosamine oligosaccharide protein conjugate is as shown in formula (III). The glucosamine oligoglycoprotein conjugate with a clear structure is prepared by utilizing a chemical synthesis method. An enzyme-linked immunosorbent assay experiment result shows that serum of a positive immune mouse and glucosamine oligosaccharides with different chain lengths have different recognition effects, and the glucosamine oligoglycoprotein conjugate can excite an antigen-specific IgG antibody with very high titer and an IgM antibody with high titer, induce a strong T-cell dependent protective immunoreaction, and can be used for preparing the immunosorbent assay kit. After immunization, serum can be obviously combined with inactivated riemerella anatipestifer. Therefore, the glucosamine oligosaccharide protein conjugate provided by the invention has relatively high application value in preparation of vaccines for resisting riemerella anatipestifer infection and drug development. In addition, the preparation steps are simple, and the yield is high.

This invention proposes an immunomicrofluidic chip for the joint detection of IgM and IgG antibodies against the same pathogen and its application. The chip includes a substrate and a cover plate pressed onto the substrate. The substrate and cover plate together form a microchannel. From left to right, the microchannel has a labeled area and a detection area. The detection area includes an IgG detection area and an IgM detection area. The upper surface of the substrate, corresponding to the labeled area, is coated with labeled anti-human IgG antibody, and the upper surface of the substrate, corresponding to the IgG detection area, is coated with recombinant pathogen antigen. The lower surface of the cover plate, corresponding to the labeled area, is coated with labeled antigen, and the lower surface of the cover plate, corresponding to the IgM detection area, is coated with anti-human IgM antibody. By integrating the IgM antibody capture detection area and the IgG antibody indirect detection area into separate fluid layers of the chip, simultaneous but non-interfering detection of IgM and IgG antibodies in the same channel can be achieved after a single sample injection.

This application relates to a method, kit, and application for detecting IgM antibodies. The method includes the following steps: mixing a hydrolysis reagent containing a proteolytic enzyme and an immunoassay reagent capable of specifically binding to the target IgM antibody in the test sample with the test sample to obtain a test reaction solution; detecting the signal value of the reaction solution; wherein the proteolytic enzyme can destroy the structure of interfering proteins in the test sample. The solution provided in this application can reduce or eliminate the interference of matrix effects produced by interfering proteins, thereby improving the detection accuracy of IgM antibodies.

Disclosed herein are immunoassay methods and reagents for detecting anti-Zika IgM antibody in a biological sample from a subject and / or diagnosing Zika virus infection in a subject. Also disclosed are algorithms for implementing the disclosed methods. The disclosed immunoassay methods, reagents, and algorithms enable efficient and reliable qualitative detection of anti-Zika virus antibodies and rapid determination of presumptive positive results for Zika virus infection in human subjects.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of HSV-I, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of an IgG monoclonal antibody specifically combined with herpes simplex virus HSV-I, the IgG monoclonal antibody is of a mouse source or a rabbit source, and the IgG antibody is modified to obtain the recombinant IgM antibody. The IgG antibody and the recombinant IgM antibody are not only suitable for a chemiluminescence method, but also show expected sensitivity magnification times in other immunological detection methods, and are helpful for realizing low-abundance detection of the specific binding herpes simplex virus HSV-I.

The present disclosure provides a photo-activated chemiluminescence detection kit and method for detecting hepatitis B virus core IgM type antibody. The detection kit comprises: reagent 1, luminescent microspheres coated with hepatitis B core antigen (HBcAg), which can react with singlet oxygen and generate a detectable light signal; reagent 2, anti-IgM antibody labeled with a first partner; and reagent 3, blocking reagent, which can reduce or eliminate interfering antibodies. The detection kit of the present disclosure destroys the structure of interfering antibodies by chemical and / or biological methods, thereby weakening or even eliminating the signal or shielded signal caused by interfering antibodies, which can significantly improve the accuracy of hepatitis B virus core IgM type antibody detection.

The present disclosure relates to an anti-human IgM antibody, its preparation method, and use. The anti-human IgM monoclonal antibody prepared in the present disclosure has high reactivity to human IgM and has good blocking and immune interference elimination effects in immunoassays, providing an important source of blocking agent raw materials.