The invention discloses PCV2
virus-like particles as well as a preparation method thereof and splitting and VLP
assembly buffer liquor. Based on an autonomous optimization design, a PCV2 nucleocapsid
protein gene which is suitable for efficiently expressing in a
prokaryotic expression system is artificially synthesized, a full-length
gene sequence of the PCV2 nucleocapsid
protein gene is expressed by an
escherichia coli
prokaryotic expression system, and the
virus-like particles are efficiently and autonomously assembled by utilizing soluble nucleocapsid
protein of the full-length
gene sequence under a special condition. An innovation point of the invention is that PCV2VLPs are obtained by utilizing the
prokaryotic expression system instead of adopting the conventional method for obtaining VLPs through an eukaryotic expression system; the method is low in cost, simple and efficient, and suitable for large-scale industrial application; moreover, an innovative buffer liquor formula integrating double functions, which not only can promote
thallus splitting, but also can be suitable for self-assembling of VLPs, is also applied; besides, the PCV2
virus-like particles obtained in the invention are very highly similar with
wild type virus in outline and good in
immunogenicity, and can be applied to developing a
subunit vaccine and a
drug delivery carrier with utilization potentiality of
porcine circovirus.