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42 results about "Hantavirus" patented technology
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A viral infection spread by rodents to people.
Antiviral activity from medicinal mushrooms
Compounds having unique antiviral properties are prepared from medicinal mushroom mycelium, extracts and derivatives. The compositions are derived from Fomitopsis, Piptoporus, Ganoderma and blends of medicinal mushroom species and are useful in preventing and treating viruses including Orthopox viruses, influenza, avian influenza, Venezuelan Equine Encephalitis, yellow fever, West Nile, Dengue, New World and Old World arenaviruses, hantavirus, Rift Valley fever, sandfly fever, hantavirus, SARS, Rhinovirus and other viruses.
Owner:TURTLE BEAR HLDG LLC
Kidney syndrome blooding diagnosis test paper strip, preparing method and detection reagent kit thereof
The invention provides a diagnostic strip for hemorrhagic fever with renal syndrome, and a preparation method and a detection reagent kit thereof. The diagnostic strip is a colloidal gold immunochromatographic strip which is formed by that a sample absorption pad (1), a colloidal gold bonding pad (2), a pyroxylin film (5) and a water absorption pad (6) are sequentially stuck on a PVC soleplate (7) in a mutual lapping way. The diagnostic strip is characterized in that the colloidal gold bonding pad (2) is composed of glass-fiber membranes of goat anti-human Mu-chain immune gold complexes containing colloidal gold markers, a detection area (3) and a quality control area (4) are arranged on the pyroxylin film (5), the position of the detection area (3) is coated with hantavirus nucleocapsid protein antigens, and the position of the control area (4) is coated with rabbit anti-goat IgG antibodies. The diagnostic strip has good specificity, sensitivity and repeatability, can realize the early and rapid diagnosis of HFRS, and is easy to carry; and the operation is simple, the cost is low, and the response is fast. The invention is easy to be popularized in primary medical units.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Degenerate reverse transcription-polymerase chain reaction (RT-PCR) detection reagent and kit for hantavirus group
InactiveCN102382907AStrong specificityIncreased sensitivityMicrobiological testing/measurementOligonucleotide primersInverse polymerase chain reaction
The invention discloses a degenerate reverse transcription-polymerase chain reaction (RT-PCR) detection reagent and a kit for hantavirus group. The detection reagent comprises a pair of degenerate heterozygous oligonucleotide primers, and the sequences of the degenerate heterozygous oligonucleotide primers are respectively G1: GCAACAGCAACATGGTTTcartaytayac and G2: CTTCTTCATTCATATTTCCATGCarnccyttytc; the non-merger consensus sequence of the 5' ends in the primers plays a role in stabilizing the combination of a 3' merger core area and a template under the condition that the degeneracy of the primers is not increased, so that the specificity of the degenerate PCR reaction is improved, various viruses in hantavirus can be amplified and detected, the homologous unknown viruses of the extendedgenes can also be detected and the amplified target fragments can be sequenced by the detection reagent; and the kit are relatively high in sensitivity and good in hantavirus group specificity, can be used for detecting domestic popular Hantaan and Seoul viruses, and can also be used for detecting other oversea epidemic strains. The degenerate RT-PCR detection reagent and the kit for the hantavirus group are high in sensitivity, good in hantavirus specificity and universal.
Owner:中华人民共和国大榭出入境检验检疫局
Primer, kit and method for conducting genetic typing on hantavirus by means of PCR direct sequencing method
ActiveCN103484566AEasy and fast genotypingMicrobiological testing/measurementMicroorganism based processesTotal rnaDirect sequencing
The invention discloses a primer, kit and method for conducting genetic typing on the hantavirus by means of a PCR direct sequencing method. The primer comprises an upstream primer: ATTAGCCCWGTCATGAGTGT, and a downstream primer: CTTTGACTCYTTTGKYTCCA, wherein the Y is a C or a T, the W is an A or a T, and the K is a G or a T. The method comprises the steps of extracting a total RNA of a virus sample to be tested, conducting reverse transcription to obtain a cDNA, using the cDNA as a template, utilizing the primer for conducting PCR amplification, conducting sequencing on an amplified target fragment, constructing a phylogenetic tree by using corresponding fragments of known genotype representative strains as a reference on the basis of sequence information of the target fragment, and conducting the genetic typing on the virus sample to be tested according to the phylogenetic tree. When the primer is used, a specificity sequence of an S gene of the hantavirus can be obtained by only conducting one-time PCR amplification, and the genetic typing can be easily, conveniently and rapidly carried out on the hantavirus by utilizing the specificity sequence.
Owner:嘉兴实践医学科技有限公司
Hantavirus ultra-fast fluorescent PCR detection kit and primer probe combination thereof
InactiveCN109355433AGuaranteed reliabilityImprove accuracyMicrobiological testing/measurementDNA/RNA fragmentationPositive controlTE buffer
The invention relates to a Hantavirus ultra-fast fluorescent PCR detection kit and a primer probe combination thereof. The Hantavirus ultra-fast fluorescent PCR detection kit comprises an extraction reagent, an amplification reagent and a control reagent; the extraction reagent is composed of sodium dodecyl sarcosinate|sodium N-Lauroyl sarcosinate (NLS) [(W / V)NLS], dithiothreitol DTT, TE buffer liquid and an NP-40 surfactant; the amplification reagent includes an RT-PCR reaction solution mixed with Hantavirus and a detection reagent of a 16-pore microfluidic chip; the control reagent comprisesa negative control and a positive control; the detection reagent comprises the RT-PCR reaction solution and is composed of Buffer, 2.0 mM dNTPs, 1 U / MuL of Taq DNA polymerase, 2 U / MuL of M-MLV reverse transcriptase, 0.3 U / MuL of RRI, the Hantavirus and a human internal standard gene detection primer probe. The negative control includes DEPC water, and the positive control includes artificially constructed pseudo-viruses containing fragments of target genes.
Owner:南通国际旅行卫生保健门诊部
Molecular clones producing recombinant DNA antigens of the hantavirus-associated respiratory distress (HARDS)
The invention provides HARDS virus rDNA for expression in molecular clones. The expressed products are useful in immunodiagnostics, prophylactics, and therapeutics for the HARDS virus and related hantaviruses. Of particular interest are a type-specific epitope of the HARDS virus G1 protein, and dominant epitopes of the HARDS virus N protein cross-reactive with antibodies to the HARDS virus and the related hantavirus PHV, both expressed by cDNA clones according to the invention.
Owner:STC UNM
Rapid test paper bar for testing colloidal gold of antibody of epidemic hemorrhagic fever virus
InactiveCN1963516AQuick screeningSave manpower and material resourcesMaterial analysisAntigenGlass fiber
This invention provides one test bar, which comprises the following steps: a, covering antibody IgM linkage single clone antibody and anti-flu hemorrhagic fever virus reset antigen multiple clone antibody two NC fiber films; b, comprising glue gold label flu hemorrhagic fever virus glass fiber film; applying film analysis and glue gold label technique to test sample flu virus antibody.
Owner:BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
Hantavirus (HTV) long-peptide vaccine
PendingCN110548136AImproving immunogenicityImprove securitySsRNA viruses negative-senseViral antigen ingredientsDiseasePeptide vaccine
The invention relates to immunogenic long peptides derived from hantavirus (HTV), or a long-peptide composition, provides a method for inducing or enhancing HTV specific immune response, and further relates to a vaccine and method used for preventing and / or treating HTV-related diseases, wherein the vaccine includes the peptides.
Owner:王美亮
Adenovirus high expression vector for improving expression of hantavirus fusion protein G1S0.7
InactiveCN101812478AHigh expressionImprove expression levelFermentationVector-based foreign material introductionForeign proteinTransfer vector
The invention relates to reconstruction of an adenovirus transfer vector, in particular to reconstruction of a transfer vector of mosaic gene G1S0.7 of hantavirus (HV) serving as hemorrhagic fever with renal syndrome (HFRS) pathogen for improving the expression of hantavirus fusion protein G1S0.7. The adenovirus transfer vector G1S0.7-pShuttle containing the mosaic gene G1S0.7 is reconstructed by using the gene recombination technology. The reconstruction comprises the following steps: replacing a CAG promoter / enhancer for a CMV promoter; or inserting a WPRE transcriptional control element at the 3' end of the promoter; or replacing the promoter and inserting a WPRE control element. The vector expresses the fusion proteins G1S0.7 respectively, and compares the expression level of foreign protein in each recombinant adenovirus. The result shows that the vector can effectively improve the expression of the fusion protein G1S0.7 compared with the primary transfer vector pShuttle, and has most obvious effect of replacing a promoter group.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Adenovirus high expression vector for improving expression of hantavirus fusion protein G2S0.7
InactiveCN101812479AHigh expressionImprove expression levelFermentationVector-based foreign material introductionForeign proteinTransfer vector
The invention relates to reconstruction of an adenovirus transfer vector, in particular to reconstruction of a pShuttle transfer vector of mosaic gene G2S0.7 of hantavirus (HV) serving as hemorrhagic fever with renal syndrome (HFRS) pathogen for improving the expression of fusion protein G2S0.7. The adenovirus transfer vector G2S0.7-pShuttle containing the mosaic gene G2S0.7 is reconstructed by using the gene recombination technology. The reconstruction comprises the following steps: replacing a CAG promoter / enhancer for a CMV promoter; or inserting a WPRE transcriptional control element at the 3' end of the promoter; or replacing the promoter and inserting a WPRE control element. The vector expresses the hantavirus fusion proteins G1S0.7 respectively, and compares the expression level of foreign protein in each recombinant adenovirus. The result shows that the vector can effectively improve the expression of the fusion protein G2S0.7 compared with the primary transfer vector pShuttle, and has most obvious effect of replacing a promoter group.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Recombinant adenovirus high-expression vector for promoting effective presentation of Hantavirus fusion protein G2S0.7
InactiveCN102618578ARaise the level of immune responseFermentationGenetic engineeringTransfer vectorDigestion
The invention relates to a recombinant adenovirus high-expression vector for promoting effective presentation of Hantavirus fusion protein G2S0.7. A G2S0.7-pCAG transfer vector containing G2S0.7 mosaic gene of Hantavirus 76-118 strain and a CAG promoter / enhancer is mainly transformed to promote the effective presentation of the high-expression Hantavirus fusion protein Hantavirus in an organism. By a gene recombination technology, the recombinant adenovirus transfer vector G2S0.7-pCAG containing the mosaic gene G2S0.7 is transformed, and Ub gene is connected to the vector. The recombinant transfer vector and the adenovirus vector are respectively subjected to double digestion, and a recombinant fragment Ub-G2S0.7-pCAG is integrated into the DNA of the adenovirus vector; and after packaging, purification and titer detection, rAd-Ub-G2S0.7-pCAG and recombinant adenovirus rAd-G2S0.7-pCAG containing the G2S0.7 mosaic gene and the CAG promoter / enhancer, which is constructed early by the inventor are respectively used for immunizing C57BL / 6 mice, immunological characteristics are researched, and results show that compared with the conventional recombinant adenovirus, the recombinant adenovirus can effectively improve partial humoral immune response level and partial cellular immune response level of organisms of experimental animals.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Recombinant adenovirus high expression vector for promoting effective presentation of hantavirus fusion protein G1S0.7
InactiveCN102676583ARaise the level of immune responseFermentationVector-based foreign material introductionCell immunityTransfer vector
The invention relates to a ubiquitin Ub-containing recombinant adenovirus high expression vector for promoting effective presentation of a hantavirus fusion protein G1S0.7. Primarily, G1S0.7 mosaic genes of hantavirus-containing 76-118 strains and a G1S0.7-pCAG transfer vector of a CAG (Cytotoxin Associated Gene) promoter / enhancer are constructed for promoting effective presentation of highly expressed hantavirus fusion protein G1S0.7 by an organism. Compared with the recombinant adenovirus, partial humoral immunity response level and partial cell immunity response level of an animal organism can be effectively improved.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Application of compound AN-329 for inhibiting releasing and diffusing of hantavirus
ActiveCN111803485AInhibition releaseInhibited DiffusionOrganic active ingredientsAntiviralsAntigenIntracellular
The invention discloses application of a compound AN-329 for inhibiting releasing and diffusing of a hantavirus. The compound AN-329 can inhibit a mutual function of hantavirus Gn and a host ESCRT (Endosomal sorting complex required for transport), a certain foundation is laid for researching the life cycle of the hantavirus as well as the mutual action mechanism of the virus and the host, and a thought is provided for researching a medicine capable of resisting the hantavirus. An experiment proves that AN-329 post cells are added, an HTNV (hantavirus) antigen quantity in cells subjected to secondary infection by supernatant is obviously lowered, a nucleic acid level of the HTNV in the cells subjected to secondary infection is obviously low, and the small molecule compound AN-329 effectively inhibit the releasing and the diffusing of the hantavirus.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
RT-PCR quantitative detection method for hantavirus
InactiveCN107557495AEasy to operateShort reaction timeMicrobiological testing/measurementMicroorganism based processesFluorescenceQuantitative determination
The invention provides a RT-PCR quantitative detection method for hantavirus. The method comprises the following steps: designing a PCR amplification primer and probe by an S gene sequence of hantavirus; with a COS-1 cell of hantavirus as a host cell, extracting RNA; performing PCR amplification on the S gene fragment of a cDNA product; connecting the PCR amplification product to a pMD18-T carrier; constructing recombinant plasmids and extracting the same; determining the copy number of the recombinant plasmids; diluting the same to obtain a plurality of standard articles of various concentrations; amplifying the plurality of standard articles by means of a real-time PCR program; detecting by fluorescence detection to obtain a Ct value; and establishing a standard curve by taking the Ct value and the concentration value of the standard articles as coordinates. The method provided by the invention tests hantavirus quantitatively and a method of sensitively and specifically detecting theviral load of hantavirus is established. The method has the advantages of simple operation, short reaction time, low cost and the like, and is in particularly suitable for clinical and scientific research.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV
Kit for detecting Hantaan-type hantavirus and detection method thereof
InactiveCN112981007AHigh sensitivityImprove accuracyMicrobiological testing/measurementMicroorganism based processesHantavirusGene
The invention relates to a kit for detecting Hantaan-type hantavirus and a detection method thereof, the kit contains a primer for detecting and typing the Hantaan-type hantavirus, the primer is designed aiming at a Hantaan-type L gene segment, a Hantaan-type S gene segment and a Hantaan-type M gene segment, and each type relates to two target probe sets and one internal standard probe set.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
Hantavirus integrated nucleic acid detection kit and detection method
InactiveCN104946800AEasy to storeEasy to transportMicrobiological testing/measurementAgainst vector-borne diseasesBuffer solutionHantavirus
The invention discloses a hantavirus integrated nucleic acid detection kit. Specifically, a hantavirus nest PCR first-round (RT-PCR) reaction system and a nest PCR second-round (a Seoul type and a Hantaan type) reaction system and reaction conditions are included. According to the detection kit, Enzyme Mix or DNA Polymerase needed for hantavirus detection, a reaction buffer solution 2*Buffer or 10*PCR Buffer, nucleotide 4*dNTP, and primers HTV-MFO, HTV-MRO, SEO-MF, SEO-MR, HMF and HMR needed for RT-PCR and PCR (a Seoul type and a Hantaan type) are integrated into a 200-microliter PCR tube, and an integrated RT-PCR (PCR) tube is formed through vacuum drying. The hantavirus integrated nucleic acid detection kit has the advantages of being high in sensitivity, high in specificity, good in stability and the like. When the hantavirus integrated nucleic acid detection kit is used for detecting rats and other samples, the detecting period is remarkably shortened, work procedures are simplified, workloads are reduced, cost is reduced, and a user can conveniently use the detection kit.
Owner:宋锋林 +1
Kit for rapidly detecting hantavirus SEOV-S4 subtype and detection method thereof
ActiveCN113913557AHigh sensitivityStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationBase JGenetics
The invention discloses a kit for rapidly detecting hantavirus SEOV-S4 subtype and a detection method thereof. The method comprises a specific isothermal amplification primer and a corresponding rProbe probe, wherein the specific isothermal amplification primer is designed according to a conservative S fragment nucleic acid sequence of the hantavirus SEOV-S4 subtype, and the rProbe probe can be digested by RNase H and contains RNA basic groups; and a fluorophore and a quenching group are respectively designed at two ends of the rProbe, RNA basic groups are cut through RNase to be free, the hantavirus SEOV-S4 subtype can be rapidly detected by utilizing the activity of DNA polymerase and efficient isothermal amplification primers and probes, and the method has the advantages of high sensitivity, stability and specificity and very high practical application value.
Owner:中国人民解放军东部战区疾病预防控制中心
A primer probe set, a kit and an application for quantitatively detecting murine infectious agents
The application provides a primer probe set, a kit and application for quantitatively detecting murine infectious agents, and belongs to the technical field of genetic engineering.The six-reagent quantitative detection kit (fluorescent PCR method) for tsutsugamushi oriental body, fever with thrombocytopenia syndrome virus, prrillonella, hantavirus, leptospira, and rickettsia mooseri can detect the six pathogenic targets of tsutsugamushi oriental body, fever with thrombocytopenia syndrome virus, prrillonella, hantavirus, leptospira, and rickettsia mooseri in one reaction, and accurately quantitatively analyzes each pathogenic target.Through single-tube reaction, parallel detection and quantification of the six pathogenic targets are realized, the time consumed due to misjudgment of the targets is reduced, the consumption of consumables and the occupation of instrument space caused by multi-tube reaction are reduced.The quantitative results reflect the pathogenic load, and provide efficient detection and application support for rapid identification and quantitative monitoring of samples of murine origin or samples of the environment related to mouse activities.
Owner:GUANGZHOU SHENGLING MEDICAL TECH CO LTD +1
Human monoclonal antibodies to hantavirus and methods of use therefore
The present disclosure is directed to antibodies binding to and neutralizing hantavirus and methods for use thereof.
Owner:VANDERBILT UNIV
Antibodies to Andes hantavirus, and methods for using same
ActiveUS11359006B2Reduce the possibilityImmunoglobulins against virusesAntiviralsHeavy chainAndes virus
This invention provides isolated human antibodies and recombinant proteins comprising defined heavy chains and light chains, wherein the antibodies and recombinant proteins neutralize Andes Virus with defined IC50 values. This invention also provides related pharmaceutical compositions, treatment methods and kits.
Owner:ICHOR BIOLOGIES LLC +1
Bivalent vaccine for hemorrhagic fever with renal syndrome and its preparation method
InactiveCN102618504BRepresentativeViral antigen ingredientsMicroorganism based processesHaemorrhagic feverHantavirus
The invention discloses a vaccine for hemorrhagic fever with renal syndrome (HFRS) and its preparation method. The vaccine is an inactivated virus of Hantavirus HV004 strain, and the virus has a preservation number of: CCTCC No. v201139. The inactivated virus can effectively prevent and treat HFRS. In addition, the invention also discloses a bivalent vaccine consisting of inactivated virus HV004 and Hantavirus Hubei-I strains. Experiments show that the bivalent vaccine has significant effects in prevention and treatment of HFRS. The invention also provides a preparation method of the vaccine, and the method includes virus proliferation, inactivation, purification and other steps. The bivalent vaccine of the invention provides an effective approach to prevention and treatment of HFRS, and plays an important role in controlling prevalence of the disease.
Owner:WUHAN UNIV
Hantavirus vaccine and preparation method thereof
The invention discloses a hantavirus vaccine and a preparation method thereof. The invention provides an mRNA vaccine against hantavirus. The mRNA vaccine contains HTNV mRNA and / or SEOV mRNA. The invention also provides an mRNA vaccine aiming at hantavirus, wherein the mRNA vaccine contains the HTNV mRNA nano particles and / or the SEOV mRNA nano particles. The HTNV + SEOV bivalent mRNA vaccine prepared by the inventor can be immunized by one injection, the immune effect of the HTNV + SEOV bivalent mRNA vaccine can be superior to that of two injections of commercially available inactivated vaccines and is not inferior to that of three injections of commercially available inactivated vaccines, and the immune persistence is relatively good. The vaccine provided by the invention has a wide market prospect.
Owner:TSINGHUA UNIVERSITY
Degenerate reverse transcription-polymerase chain reaction (RT-PCR) detection reagent and kit for hantavirus group
InactiveCN102382907BStrong specificityIncreased sensitivityMicrobiological testing/measurementOligonucleotide primersHantavirus
Owner:中华人民共和国大榭出入境检验检疫局
Recombinant adenovirus expression vector containing heat shock protein HSP70C for promoting hantavirus fusion protein G2S0.7 presentation
InactiveCN102676582ARaise the level of immune responseImprove submission efficiencyFermentationGenetic engineeringTransfer vectorOrganism
The invention relates to a recombinant adenovirus expression vector containing heat shock protein HSP70C for promoting hantavirus fusion protein G2S0.7 presentation. A G2S0.7-pCAG transfer vector containing CAG promoter / enhancer and G2S0.7mosaic gene of hantavirus 76-118 strain is mainly built to promote organism to effectively present highly expressed hantavirus fusion protein G2S0.7. According to the invention, humoral immune response level and cellular immune response level of an experimental animal body can be effectively improved.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Hantavirus glucoprotein specific T cell epitope peptide and application thereof
ActiveCN102731617BStrong immune responseViral antigen ingredientsImmunoglobulins against virusesCtl epitopeViral glycoprotein
The invention discloses an HTNV-Gn / Gc specific T cell epitope peptide and an application thereof. The amino acid sequence of the HTNV-Gn / Gc specific T cell epitope peptide is one selected from SEQ ID NO. 1 to SEQ ID NO. 75. The HTNV-Gn / Gc specific T cell epitope peptide comprises CD4<+>T cell epitope and CTL epitope, and can respectively induce CD4<+>T cell and CD8<+>T cell to generate intensive cellular immunologic response and to secrete high-level IFN-gamma. The HTNV-Gn / Gc specific T cell epitope peptide can be used in the preparation of T cell epitope peptide vaccines, and can be used in inducing CD4<+>T cell or cytotoxicity T cell generating epitope peptide specificity. Therefore, the HTNV-Gn / Gc specific T cell epitope peptide has a good exploitation and application prospect in the field of HFRS specific immunotherapy.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Joint detection lateral chromatography method for hemorrhagic fever multi-pathogen nucleic acid
PendingCN120738399AMicrobiological testing/measurementBiological material analysisUltraviolet lightsHaemorrhagic fever
The invention relates to the technical field of pathogen detection, in particular to a hemorrhagic fever multi-pathogen nucleic acid joint detection lateral chromatography method which comprises the following steps: performing sequence alignment, target gene determination and primer screening based on hantavirus or dengue virus; an RT-PCR detection system is constructed; adding a reaction product of an RT-PCR detection system into the diluent, fully blowing, sucking and uniformly mixing, and then completely dropwise adding into a sample hole of the FAM immunochromatography test strip for amplification, so as to obtain an amplification product; the detection result is positive, only the quality control line emits the red light or the quality control line does not emit the light, the detection is invalid, the method is simple and convenient to operate, the process from sample treatment to result interpretation can be completed within one hour, and the detection is rapid. The problems that a traditional detection method is long in detection time, and multiple pathogens cannot be identified at the same time are solved.
Owner:CHONGQING CENT FOR DISEASE CONTROL & PREVENTION (CHONGQING EMERGENCY TREATMENT CENT FOR DISASTER RELIEF & DISEASE PREVENTION) +1
Bivalent vaccine for hemorrhagic fever with renal syndrome and its preparation method
InactiveCN102618504ARepresentativeSelect targetingViral antigen ingredientsMicroorganism based processesHaemorrhagic feverHantavirus
The invention discloses a vaccine for hemorrhagic fever with renal syndrome (HFRS) and its preparation method. The vaccine is an inactivated virus of Hantavirus HV004 strain, and the virus has a preservation number of: CCTCC No. v201139. The inactivated virus can effectively prevent and treat HFRS. In addition, the invention also discloses a bivalent vaccine consisting of inactivated virus HV004 and Hantavirus Hubei-I strains. Experiments show that the bivalent vaccine has significant effects in prevention and treatment of HFRS. The invention also provides a preparation method of the vaccine, and the method includes virus proliferation, inactivation, purification and other steps. The bivalent vaccine of the invention provides an effective approach to prevention and treatment of HFRS, and plays an important role in controlling prevalence of the disease.
Owner:WUHAN UNIV
Anti-hantavirus NP protein antibody pair and application thereof
PendingCN121991209AImmunoglobulins against virusesFermentationProtein detectionEpidemiological Monitoring
The invention relates to the technical field of biology, in particular to an anti-hantavirus NP protein antibody pair and application thereof. The protein detection limit of a double-antibody sandwich ELISA detection system established on the basis of the antibody pair is 1ng / mL, the antibody pair has good performance in the aspects of linear range, specificity, accuracy and the like, and an effective tool is provided for early clinical diagnosis, epidemiological monitoring, virus load evaluation and related scientific research of hantavirus.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI +1
Antibodies to andes hantavirus, and methods for using same
ActiveUS20200087381A1Reduce the possibilityImmunoglobulins against virusesAntiviralsHeavy chainAndes virus
This invention provides isolated human antibodies and recombinant proteins comprising defined heavy chains and light chains, wherein the antibodies and recombinant proteins neutralize Andes Virus with defined IC50 values. This invention also provides related pharmaceutical compositions, treatment methods and kits.
Owner:ICHOR BIOLOGIES LLC +1
Advanced epitope peptides of hantavirus envelope glycoprotein, and coding gene and application thereof
PendingCN114478719AUnderstanding ImmunobiologyEasy to useSsRNA viruses negative-senseViral antigen ingredientsImmunityMicrobacterium
The invention belongs to the technical field of microbial immunity, and particularly relates to dominant epitope peptides of hantavirus envelope glycoprotein as well as a coding gene and application of the dominant epitope peptides. The epitope peptide is composed of sequences as shown in SEQ ID NO.1-11. The invention further discloses a preparation method of the epitope peptide. According to the invention, 11 candidate epitopes are obtained by screening and verification through a method for predicting and identifying antigen-specific optimized epitopes of HTNV GP, and then verification is carried out by simulating molecular docking. The immunoreactivity of the epitope in different isolates around the world is deeply compared and analyzed to verify the stability of the epitope in vaccine application. In a word, the generic MHC-I immunoreactivity of the HTNV GP is comprehensively evaluated according to comparative immunology, so that the immunobiology of the HTNV GP can be understood, and guidance is provided for development of HTNV epitope vaccines in the future.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY