70 results about "Leptospira" patented technology

The invention discloses a detecting method for leptospira, which is based on the quantitative detection technique of nucleic acid isothermal expansion, which is characterized in that a specific primer is used, and the specific area of target gene is expanded utilizing the LAMP technological platform; under the assistance of a series of quality control and internal control detection system, the leptospira is detected in molecule level, which can realize the qualitative and quantitative detection to micro sample. Compared with the prior art, the detecting method for leptospira has the advantages of decreased non-specific expansion because of isothermal expansion, good specificity, fast detection speed, high sensitivity, no need for special equipment, low cost, easy operation, easy popularization and wide application prospect, and is suitable for experimental study, clinical detection and environment detection.

The invention provides a pink plumepoppy herb injection and a preparation method thereof; the pink plumepoppy herb injection is prepared by the raw materials with the following parts by weight: 30-40 parts of pink plumepoppy herb extract, 0-30 parts of latent solvent, 0.5-5 parts of anodyne and 25-70 parts of dispersion medium; the pink plumepoppy herb extract is prepared by the following procedure: pink plumepoppy herb is percolated with sulphuric acid, the percolate is subject to alcohol precipitation and is filtered and then ethanol is recycled; the pink plumepoppy herb injection of the invention can effectively restrain the growth of Gram-positive bacteria and has stronger bacteriostatic action than berberine, and diplococcus pneumoniae, staphylococcus aureus and bacillus subtilis are sensitive to the pink plumepoppy herb injection; meanwhile, the pink plumepoppy herb injection also has the function of resisting nematodes and leptospira and can inhibit and slow the body temperature raise caused by diplococcus pneumoniae; furthermore, the pink plumepoppy herb injection has the efficacies of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation; the indications are yellow and white scour of piglets; the preparation method has convenient operation, high yield, low cost and strong stability of finished products.

The present invention discloses a method to identify and quantify environmental microorganisms useful in biomining processes. These microorganisms are basically 10, belonging to Bacteria: Acidiphilium sp., Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans; and Archaea: Acidianus sp., Ferroplasma sp., Metallosphaera sp., Sulfolobus sp. and Thermoplasma sp.The method comprises performing a PCR with specific primers designed in our laboratories for different taxons SEQ ID No. 4 to SEQ ID No.: 407. With qPCR results and other data obtained from the analyzed sample, the microorganism concentration of each analyzed taxon present in the sample is calculated using a mathematical formula.

The invention relates to a fluorescence quantification PCR (Polymerase Chain Reaction) primer, a fluorescence quantification PCR probe and a fluorescence quantification PCR kit for detecting dog leptospira nucleic acid. The primer and the probe are shown as SEQ ID NO. (Sequence Identification Number) 1-4. The kit comprises 22 microliters of 5xFRET (Fluorescence Resonance Energy Transfer)-qPCRStock and 22 microliters of 5xOligo mixture, wherein the Oligo mixture comprises the primer and the probe. With the adoption of the primer and the probe, the pathogenic dog leptospira can be detected quickly, specifically and sensitively.

Leptospira outer membrane proteins (OMPs) LP1454, LP1118, LP1939, MCEII, CADF-like1, CADF-like2, CADF-like3, Lp0022, Lp1499, Lp4337, Lp328 or L21 are provided. The OMPS can be used as tools for developing effective vaccines or diagnostic methods for leptospirosis. Expression vectors for the OMP genes are further provided. The antigenic properties of the Leptospira OMPs can be used to create, manufacture or improve vaccines. Vaccines, including but not limited to DNA vaccines, recombinant vaccines, and T-cell epitope vaccines based on the foregoing OMPs are also provided. Methods for producing such vaccines are also provided. Also provided are methods for using Leptospira OMP genes, proteins and antibodies for therapeutic treatment and serological diagnosis techniques.

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and / or borrelia burgdorferi and / or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.

The invention provides a kit for rapid mass spectrometric detection of leptospira pathogenicity (toxicity) and relates to the technical field of protein fingerprinting detection. The kit is characterized in that based on a mass spectrometry technology, a specific polypeptide mass spectrum model for rapid identification of pathogenicity of leptospira is constructed and by the mass spectrometry technology, pathogenicity detection is carried out. The kit can realize identification of pathogenic (toxic) and non-pathogenic (nontoxic) leptospira by a specific fingerprint, has high accuracy and good repeatability, can realize detection of 96 samples in 2h, can solve the problem that pathogenicity detection of mass leptospira consumes labor and time, and has a low cost and a good application prospect.

A copper bioleaching process which makes use of a consortium which contains Leptospirillum ferriphilum and a sulphur oxidising microorganism which is halophilic or halotolerant.

The invention discloses a method for detecting leptospira by multiple cross amplification in combination with a polymer nano-biosensor. The method uses specially designed multi-cross displacement amplification primers, and also provides a primer formed by labeling the 5' end of the amplification primer C1 with biotin and a primer formed by labeling the 5' end of the amplification primer D1 with hapten. A product of the amplification of the LipL41 gene of pathogenic leptospira in the method can be visually detected by the polymer nano-biosensor. The method is convenient, fast, sensitive and specific, and is suitable for the specific detection of pathogenic leptospira.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia psittaci, pneumocystis carinii, leptospira, Q fever rickettsiosis and brucellosis. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention provides novel immunogenic proteins LigA and LigB from Leptospira for use in the development of effective vaccines and antibodies, as well as improved diagnostic methods and kits.

The invention provides novel immunogenic proteins LigA and LigB from Leptospira for use in the development of effective vaccines and antibodies, as well as improved diagnostic methods and kits.

The invention refers to the application of 4-deminocycline ramification to the preparation of drugs of anti-SARS, antivirus, anti-leptospira, anti-mycobacterium, etc; a medicinal combination for curing SARS contains effective amount of 4-deminocycline ramification and medicinal carrier; the invention has the application advantage of 4-deminocycline ramification able to prevent and cure the infection caused by virus ( SARS coronary virus), leptospira and mycobacterium (including mycoplasma and chlamydia).

The invention discloses a question mark dependent type leptospira replicator loss ratio analysis method. Firstly, comparison jetting-out replicator is conducted on a question mark dependent type leptospira, then replacing number of each synonymy point and each non-synonymy point of each pair of replicators is respectively calculated, and finally function fitting is conducted by using of property structure differential equation and regression analysis. Change condition of selection of constraining force is understood in the replicator evolutionary process, the concept that S is used as divergence time is introduced, and copy loss rate of the replicators is deduced.

The present invention provides a kind of new leptospiral pathogen-related protein, polynucleotide for coding said pathogen-related protein and method for producing said pathogen-related protein by utilizing recombination technique. Said invention also discloses the application of said pathogen-related protein and its code sequence.

The invention provides a leptospira outer membrane protein capable of serving as a candidate vaccine, a polynucleotide for coding the outer membrane protein and a method for generating the outer membrane protein through a recombinant technique. The outer membrane protein is a useful immunogen for preparing the vaccine. The invention also discloses application of the protein and a coded sequence thereof for preparing the vaccine.

Novel immunodominant antigenic proteins and peptides associated with associated with leptospirosis were identified using a proteome array based on expression of ORFs from a Leptospira genome. Compositions, methods, and uses of such antigenic proteins and peptides in the diagnosis and staging of leptospirosis infection and in compositions, methods, and uses of such antigenic is proteins and peptides in prophylactic and therapeutic vaccines are disclosed.

The present invention relates to a diagnostic method for the detection of acute leptospirosis. The method of the invention allows for a quick, specific and sensitive detection of the disease caused by pathogenic Leptospira. The described method is based on the immunological detection of the protein LipL21, a membrane protein expressed specifically by pathogenic strains of Leptospira. Furthermore, the present invention relates to the development of a diagnostic device that allows positive diagnosis to be conducted in the early and critical stage of the infection. The preferred diagnostic device of the invention is a lateral flow test, which as a hand-held assay, is easy to use and facilitates diagnosis of leptospirosis in the first 5 days of infection. Further disclosed is the antibody raised against the LipL21 protein as well as its respective uses in the diagnostic methods and devices. Finally, a diagnostic kit is disclosed containing the inventive materials described herein to conduct diagnosis of acute leptospirosis during the critical early stage of infection. The first 5 days of the infection is where the subject or patient could succumb to the disease. Diagnosis of the disease at this critical stage of infection would then advise the affected patient to have immediate systemic antibiotic treatment and thereby, avoid succumbing to the disease.

The invention designs a medical serology experiment device and a use method thereof, in particular a non-syphilitic leptospira experimental level reaction device and a use method thereof. Aiming at overcoming the defects existing in the prior art and method, the invention provides a non-syphilitic leptospira experimental level reaction device with standardized operation and a use method thereof. The non-syphilitic leptospira experimental level reaction device is characterized by comprising an electro-motor, a mechanical transmission box, an eccentric rotation axis connection fulcrum, a rotatable horizontal plate, an electronic control part, a key-press and an acousto-optic digital display panel and particularly characterized by comprising a transparent or semi-transparent outer cover which prevents dust, pollution and dryness, a detachable horizontal rotating plate, a mechanical transmission part box body, an electronic control assembly, related technical requirements and cautions for sound, lighting and digital display, and the combination of routinization and simplification preset memory. When in use, the non-syphilitic leptospira experimental level reaction device can be reasonably assembled and operated according to the requirement of experiment purpose.

The invention relates to detection primers and detection kits and particularly discloses a PCR (polymerase chain reaction) primer and a kit for detecting pathogenic canine leptospirosis. The PCR primer includes a forward primer: 5'-CTTGGGATTCTTCTAATMCSGATA-3' and a reverse primer: 5'-GATCCTTGTATTCCACCGATG-3'. The amplified fragment length of the PCR primer is 124bp. The PCR primer and the kit for detecting the pathogenic canine leptospirosis have the advantages that LigB genes are selected as a detection object so as to achieve the purpose of detecting the pathogenic canine leptospirosis; the PCR primer and the kit, which are used for detecting the LigB genes of the canine leptospirosis, are time saving, labor saving and excellent in specificity and sensitivity; the PCR primer and the kit can be used for early diagnosis of the canine leptospirosis during experiments, an established and assembled canine leptospirosis PCR method is high in specificity, and leptospira concentration detection sensitivity can reach 101 copies / microliters.

The invention refers to the application of 4-deminocycline ramification to the preparation of drugs of anti-SARS, antivirus, anti-leptospira, anti-mycobacterium, etc; a medicinal combination for curing SARS contains effective amount of 4-deminocycline ramification and medicinal carrier; the invention has the application advantage of 4-deminocycline ramification able to prevent and cure the infection caused by virus ( SARS coronary virus), leptospira and mycobacterium (including mycoplasma and chlamydia).

The invention relates to a fluorescence quantification PCR (Polymerase Chain Reaction) primer, a fluorescence quantification PCR probe and a fluorescence quantification PCR kit for detecting dog leptospira nucleic acid. The primer and the probe are shown as SEQ ID NO. (Sequence Identification Number) 1-4. The kit comprises 22 microliters of 5xFRET (Fluorescence Resonance Energy Transfer)-qPCRStock and 22 microliters of 5xOligo mixture, wherein the Oligo mixture comprises the primer and the probe. With the adoption of the primer and the probe, the pathogenic dog leptospira can be detected quickly, specifically and sensitively.

The invention discloses a novel antibacterial and antiviral marine actinomycete. The actinomycete is prepared through the following steps: implanting streptomycetes, small single cell bacteria, Rhodococcus rhodococcus, Nocard's bacillus and Actinoplanes in maifanite used as a carrier, pouring the maifanite into a mixer at 20-37 DEG C, mixing the maifanite for 30 min, and culturing the carrier attached with the above thalluses for 3-5 d. The antibacterial and antiviral principle of the marine actinomycete is generation of extracellular enzyme in the culturing of the streptomycetes, small singlecell bacteria, Rhodococcus rhodococcus, Nocard's bacillus and Actinoplanes attached to the maifanite. The marine actinomycete is a highly-adaptable decomposing bacterium, can resist a high temperature, a high pressure, a high salt and low nutrition, has no toxic or side effects, has anti-bacterium, antivirus, anti-Leptospira, detoxification, antitumor and leukemia cell-destruction effects, and can effectively improve the immunity.

The invention belongs to the technical field of microbiological detection, and in particular relates to a spirochete dyeing reagent and its application. The invention provides a new dyeing reagent and a method of use thereof for Leptospira dyeing. The preparation of the reagent is simple, and the cost of materials used in the preparation of the reagent is low. Easy to obtain, the prepared reagent does not require special storage conditions, and can be stored at room temperature for a long time without failure. The staining method is simple to operate and does not require high skills for the experimenter; and the staining time only takes 40-60 seconds, which greatly shortens the staining time. time, the staining effect is good, and the Leptospira dyed by this method is green, which is easier to observe under the microscope and has no impurities. It is suitable for promotion and application in staining of Leptospira.

The current invention provides a multiplex method for screening bovine samples for antibodies against several important pathogens. These pathogens include Bovine Viral Diarrhoea Virus (BVDV), Bovine Herpesvirus-1 (BoHV-1), Mycobacterium paratuberculosis (MAP), Leptospira species, Neospora caninum and Fasciola hepatica. This multiplex screening is enabled by substrates with immobilized pathogen antigens and offer multiple advantages for the routine testing of farm animals.

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in a citric acid solution. It also pertains to a vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises this composition, and to the use of citric acid to stabilize an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the citric acid in the carrier.