68 results about "Leptospira" patented technology

The invention discloses a detecting method for leptospira, which is based on the quantitative detection technique of nucleic acid isothermal expansion, which is characterized in that a specific primer is used, and the specific area of target gene is expanded utilizing the LAMP technological platform; under the assistance of a series of quality control and internal control detection system, the leptospira is detected in molecule level, which can realize the qualitative and quantitative detection to micro sample. Compared with the prior art, the detecting method for leptospira has the advantages of decreased non-specific expansion because of isothermal expansion, good specificity, fast detection speed, high sensitivity, no need for special equipment, low cost, easy operation, easy popularization and wide application prospect, and is suitable for experimental study, clinical detection and environment detection.

The invention provides a pink plumepoppy herb injection and a preparation method thereof; the pink plumepoppy herb injection is prepared by the raw materials with the following parts by weight: 30-40 parts of pink plumepoppy herb extract, 0-30 parts of latent solvent, 0.5-5 parts of anodyne and 25-70 parts of dispersion medium; the pink plumepoppy herb extract is prepared by the following procedure: pink plumepoppy herb is percolated with sulphuric acid, the percolate is subject to alcohol precipitation and is filtered and then ethanol is recycled; the pink plumepoppy herb injection of the invention can effectively restrain the growth of Gram-positive bacteria and has stronger bacteriostatic action than berberine, and diplococcus pneumoniae, staphylococcus aureus and bacillus subtilis are sensitive to the pink plumepoppy herb injection; meanwhile, the pink plumepoppy herb injection also has the function of resisting nematodes and leptospira and can inhibit and slow the body temperature raise caused by diplococcus pneumoniae; furthermore, the pink plumepoppy herb injection has the efficacies of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation; the indications are yellow and white scour of piglets; the preparation method has convenient operation, high yield, low cost and strong stability of finished products.

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and/or borrelia burgdorferi and/or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.

The invention provides a kit for rapid mass spectrometric detection of leptospira pathogenicity (toxicity) and relates to the technical field of protein fingerprinting detection. The kit is characterized in that based on a mass spectrometry technology, a specific polypeptide mass spectrum model for rapid identification of pathogenicity of leptospira is constructed and by the mass spectrometry technology, pathogenicity detection is carried out. The kit can realize identification of pathogenic (toxic) and non-pathogenic (nontoxic) leptospira by a specific fingerprint, has high accuracy and good repeatability, can realize detection of 96 samples in 2h, can solve the problem that pathogenicity detection of mass leptospira consumes labor and time, and has a low cost and a good application prospect.

The invention discloses a method for detecting leptospira by multiple cross amplification in combination with a polymer nano-biosensor. The method uses specially designed multi-cross displacement amplification primers, and also provides a primer formed by labeling the 5' end of the amplification primer C1 with biotin and a primer formed by labeling the 5' end of the amplification primer D1 with hapten. A product of the amplification of the LipL41 gene of pathogenic leptospira in the method can be visually detected by the polymer nano-biosensor. The method is convenient, fast, sensitive and specific, and is suitable for the specific detection of pathogenic leptospira.

The invention provides novel immunogenic proteins LigA and LigB from Leptospira for use in the development of effective vaccines and antibodies, as well as improved diagnostic methods and kits.

The invention refers to the application of 4-deminocycline ramification to the preparation of drugs of anti-SARS, antivirus, anti-leptospira, anti-mycobacterium, etc; a medicinal combination for curing SARS contains effective amount of 4-deminocycline ramification and medicinal carrier; the invention has the application advantage of 4-deminocycline ramification able to prevent and cure the infection caused by virus ( SARS coronary virus), leptospira and mycobacterium (including mycoplasma and chlamydia).

The invention discloses a question mark dependent type leptospira replicator loss ratio analysis method. Firstly, comparison jetting-out replicator is conducted on a question mark dependent type leptospira, then replacing number of each synonymy point and each non-synonymy point of each pair of replicators is respectively calculated, and finally function fitting is conducted by using of property structure differential equation and regression analysis. Change condition of selection of constraining force is understood in the replicator evolutionary process, the concept that S is used as divergence time is introduced, and copy loss rate of the replicators is deduced.

The present invention provides a kind of new leptospiral pathogen-related protein, polynucleotide for coding said pathogen-related protein and method for producing said pathogen-related protein by utilizing recombination technique. Said invention also discloses the application of said pathogen-related protein and its code sequence.

The invention provides a leptospira outer membrane protein capable of serving as a candidate vaccine, a polynucleotide for coding the outer membrane protein and a method for generating the outer membrane protein through a recombinant technique. The outer membrane protein is a useful immunogen for preparing the vaccine. The invention also discloses application of the protein and a coded sequence thereof for preparing the vaccine.

The present invention relates to a diagnostic method for the detection of acute leptospirosis. The method of the invention allows for a quick, specific and sensitive detection of the disease caused by pathogenic Leptospira. The described method is based on the immunological detection of the protein LipL21, a membrane protein expressed specifically by pathogenic strains of Leptospira. Furthermore, the present invention relates to the development of a diagnostic device that allows positive diagnosis to be conducted in the early and critical stage of the infection. The preferred diagnostic device of the invention is a lateral flow test, which as a hand-held assay, is easy to use and facilitates diagnosis of leptospirosis in the first 5 days of infection. Further disclosed is the antibody raised against the LipL21 protein as well as its respective uses in the diagnostic methods and devices. Finally, a diagnostic kit is disclosed containing the inventive materials described herein to conduct diagnosis of acute leptospirosis during the critical early stage of infection. The first 5 days of the infection is where the subject or patient could succumb to the disease. Diagnosis of the disease at this critical stage of infection would then advise the affected patient to have immediate systemic antibiotic treatment and thereby, avoid succumbing to the disease.

The invention designs a medical serology experiment device and a use method thereof, in particular a non-syphilitic leptospira experimental level reaction device and a use method thereof. Aiming at overcoming the defects existing in the prior art and method, the invention provides a non-syphilitic leptospira experimental level reaction device with standardized operation and a use method thereof. The non-syphilitic leptospira experimental level reaction device is characterized by comprising an electro-motor, a mechanical transmission box, an eccentric rotation axis connection fulcrum, a rotatable horizontal plate, an electronic control part, a key-press and an acousto-optic digital display panel and particularly characterized by comprising a transparent or semi-transparent outer cover which prevents dust, pollution and dryness, a detachable horizontal rotating plate, a mechanical transmission part box body, an electronic control assembly, related technical requirements and cautions for sound, lighting and digital display, and the combination of routinization and simplification preset memory. When in use, the non-syphilitic leptospira experimental level reaction device can be reasonably assembled and operated according to the requirement of experiment purpose.

The invention relates to detection primers and detection kits and particularly discloses a PCR (polymerase chain reaction) primer and a kit for detecting pathogenic canine leptospirosis. The PCR primer includes a forward primer: 5'-CTTGGGATTCTTCTAATMCSGATA-3' and a reverse primer: 5'-GATCCTTGTATTCCACCGATG-3'. The amplified fragment length of the PCR primer is 124bp. The PCR primer and the kit for detecting the pathogenic canine leptospirosis have the advantages that LigB genes are selected as a detection object so as to achieve the purpose of detecting the pathogenic canine leptospirosis; the PCR primer and the kit, which are used for detecting the LigB genes of the canine leptospirosis, are time saving, labor saving and excellent in specificity and sensitivity; the PCR primer and the kit can be used for early diagnosis of the canine leptospirosis during experiments, an established and assembled canine leptospirosis PCR method is high in specificity, and leptospira concentration detection sensitivity can reach 101 copies/microliters.

The current invention provides a multiplex method for screening bovine samples for antibodies against several important pathogens. These pathogens include Bovine Viral Diarrhoea Virus (BVDV), Bovine Herpesvirus-1 (BoHV-1), Mycobacterium paratuberculosis (MAP), Leptospira species, Neospora caninum and Fasciola hepatica. This multiplex screening is enabled by substrates with immobilized pathogen antigens and offer multiple advantages for the routine testing of farm animals.

A novel leptospiral outer membrane protein-OMP20 which is a useful immunoger for preparing vaccine, the polynucleotide for coding it, the process for preparing the said protein by recombination, and the application of the said protein and its coding sequence in preparing vaccine are disclosed.

The present invention provides a kind of new leptospiral hemolysin, polynucleotide for coding said hemolysin and method for producing said hemolysin by using recombination technique. Said invention also discloses the application of the hemolysin and its code sequence.