67 results about "Swine Erysipelas" patented technology

The invention discloses a medicine for preventing and treating porcine reproductive and respiratory syndrome (PRRS). The medicament is prepared by smashing and uniformly mixing the following main crude drugs in parts by weight: 9-11 parts of pine needles, 1.5-2.5 parts of dried tangerine or orange peel, 0.8-1.2 parts of white ginseng, 0.8-1.2 parts of scutellaria baicalensis, 0.8-1.2 parts of golden cypress, 1.5-2.5 parts of gardenia jasminoides ellis, 1.5-2.5 parts of isatis root, 0.2-0.4 part of liquorice, 1.5-2.5 parts of honeysuckle flower, 1.5-2.5 parts of oriental wormwood, 3.5-4.5 parts of milkvetch root, 1.5-2.5 parts of cyrtomium rhizome, 0.8-1.2 parts of fructus forsythiae, 1.5-2.5 parts of platycodon grandiflorum, 0.8-1.2 parts of giant knotweed rhizome and 0.3-0.5 part of milkvetch root polysaccharide. The medicament has a better preventing function on pigs which are not infected by the PRRS, has a good therapeutical effect on pigs which suffer from moderate PRRS and also has an obvious control effect on swine influenza, swine streptococcosis and swine erysipelas.

The invention discloses a drug for preventing and curing swine erysipelas and a preparation method thereof. The drug for preventing and curing swine erysipelas is prepared from, by mass parts, 140 to 165 parts of fructus forsythiae, 320 to 355 parts of folium isatidis, 220 to 260 parts of rhizoma coptidis, 240 to 270 parts of cortex phellodendri, 320 to 360 parts of fructus gardeniae, 220 to 255 parts of radix gentianae, 215 to 250 parts of cortex moutan, 230 to 275 parts of radix et rhizoma rhei, 140 to 165 parts of liquorice root and 225 to 255 parts of root, stem and leaf of Chinese mahonia. None of the raw materials in the drug provided by the invention are incompatible, and the drug can obviously lower the occurrence rate of swine erysipelas, has good preventing and curing effects on swine erysipelas, has no toxic and side effects, is safe to use, and has no harmful drug residue.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor, adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled water and refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention discloses a drug for preventing swine erysipelas and a preparation method thereof. The drug for preventing swine erysipelas is prepared from the following active ingredients in parts by weight: 130 to 165 parts of acalypha australis, 320 to 355 parts of folium isatidis, 220 to 260 parts of coptis chinensis, 240 to 270 parts of cortex phellodendri, 320 to 360 parts of fructus gardenia, 220 to 255 parts of felwort, 215 to 250 parts of cortex moutan, 230 to 275 parts of rheum officinale, 140 to 165 parts of licorice roots, 205 to 235 parts of mahonia fortunei and 120 to 150 parts of chinese fevervine herb. The raw materials in the drug have no incompatibility, the occurrence rate of swine erysipelas can be obviously decreased, and the effect for preventing and treating wine erysipelas is good.

The invention relates to traditional Chinese medicine decoction liquid for treating swine erysipelas and a preparation method thereof. The traditional Chinese medicine decoction liquid is prepared from the following ingredients in parts by weight: 10 to 15 parts of honeysuckle flowers, 10 to 15 parts of fructus forsythiae, 5 to 10 parts of dyer woad leaves, 10 to 15 parts of radix isatidis, 10 to 15 parts of radix scutellariae, 5 to 10 parts of dandelion, 5 to 10 parts of radix et rhizoma rhei, 5 to 10 parts of liquorice roots and 8 to 12 parts of radix bupleuri. The traditional Chinese medicine decoction liquid has the advantages that the efficacies of achieving antibacterial and anti-inflammation functions and clearing away heat and toxic materials are achieved; no medicine residue exists; no toxic and side effects exist; medicine resistance cannot be easily generated; the safety is high; the use is convenient; the absorption is easy; the requirements of safe animal remedy and animal-derived food safety guaranteeing are met; and the traditional Chinese medicine decoction liquid belongs to ideal traditional Chinese medicine decoction liquid for treating the swine erysipelas.

The invention provides swine erysipelas Spa A protein. The amino acid sequence of the swine erysipelas Spa A protein is SEQ ID NO: 1. The erysipelothrix rhusiopathiae Spa A protein is used for preparing vaccines or diagnostic reagents. A subunit vaccine prepared from the erysipelothrix rhusiopathiae Spa A protein has the characteristics that antigen stability is high, purity is high, specificity is high, other uncorrelated antibodies are not generated, and the detection method is convenient and accurate, and a firm basis is established for industrial production of swine erysipelas subunit vaccines and diagnostic reagents.

The invention discloses a primer and kit for efficiently detecting Erysipelothrix rhusiopathiae. According to the primer and the kit, an improved LAMP technology serves as a gene amplification reaction principle, and gold nanoparticles are added into a reaction system to adsorb ssDNA and protease and inhibit nonspecific reactions during heating, thus, the aim of warm start is achieved, and the nonspecific reactions during heating are avoided; by combining the improved LAMP technology and a microfluidic chip technology, an accurate detection result can be presented rapidly, and the aim of jointdetection on the same sample with a plurality of different indexes is achieved; and meanwhile, reaction reagents are pre-buried into a microfluidic chip, a user only needs to add a sample, and operations are simple and convenient.

The invention provides a medicine for controlling swine erysipelas, belonging to the technical field of pharmaceutical and medical engineering. The medicine comprises the following components in parts by weight: 5-15 parts of honeysuckle, 5-15 parts of dyers woad leaf, 5-15 parts of Tokyo violet herb, 5-15 parts of radix arnebiae, 4-12 parts of cyrtomium rhizome, 4-12 parts of gypsum rubrum, 4-12 parts of tree peony root bark, 4-12 parts of common anemarrhena rhizome, 3-10 parts of figwort root, 3-10 parts of spikemoss, 3-10 parts of red peony root, 3-10 parts of platycodon root, 2-6 parts of rhubarb, 2-6 parts of cape jasmine fruit and 2-6 parts of liquorice. The medicine is prepared by drying the above Chinese herbal medicines in the sun, weighing the dried medicines, grinding the weighed medicines, sieving the powder with a 60-80-mesh sieve and mixing the powder uniformly. During clinical application, the medicine is soaked in boiling water for 30-60 minutes so that the active ingredients of the medicine are fully dissolved and precipitated to be beneficial to internal absorption of sick swine, and at the same time, the medicine efficacy can be improved by adding a few sugars and Vc. The medicine has higher clinical cure rate, does not have adverse reactions, does not generate medicine resistance easily, avoids medicine residues in pork, has easily obtained needed raw materials, is simple to prepare and convenient to use and can be widely applied in the swine industry.

The invention belongs to the technical field of feed additive research and development and particularly relates to a traditional Chinese medicine feed additive for preventing and controlling swine bacterial diseases and a preparation method thereof. The traditional Chinese medicine feed additive for preventing and controlling the swine bacterial diseasesis prepared from, by weight, 60-100 parts oftraditional Chinese medicine granules and 5-20parts ofseasoning aroma enhancementpowder. The pig feed additiveis prepared by compounding a variety of traditional Chinese medicine components, adopts the pure traditional Chinese medicine ingredients and is free of toxic and side effects. The pig feed additive has the good prevention and treatment effect on the swine bacterial diseases, especiallyswine bacterial diseasessuch as swine erysipelas, swine plague, swine escherichia coli and swine red scour. The raw materials for the pig feed additive are easy to obtain,a pharmaceutical source is wide, the materials are convenient to take, and thetraditional Chinese medicine feed additive can be popularized and used.

The invention provides a method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine. The method has the advantages that bacteria solution is prepared from bacillus rhusiopathiae suis in fermentation modes in preparation procedures, a pH (potential of hydrogen) value of fermentation broth is strictly controlled in bacteria solution fermentation procedures, accordingly, the immunogenicity of bacillus rhusiopathiae suis antigens can be effectively guaranteed, and the protection rate can be higher than 90% without excessively high quantities of thalli of the bacillusrhusiopathiae suis; viruses can be reproduced from porcine parvovirus in IBRS-2 cell suspension culture modes by the aid of bioreactors in swine erysipelas and porcine parvovirus bivalent inactivatedvaccine preparation procedures, and accordingly the titer of virus liquid can be obviously improved; dissolved oxygen, pH, rotational speeds and the like are set in virus reproduction procedures, accordingly, the homogeneity of inter-assay virus liquid can be guaranteed, the shortcomings of complicated operation, high labor intensity, vulnerability to pollution and the like in spinner-bottle culture procedures can be overcome, and the quality stability of porcine parvovirus inactivated vaccine can be effectively enhanced.

The invention belongs to the technical field of research and development of feed additives, and particularly relates to a traditional Chinese medicine preparation for preventing and treating bacterial diseases of pigs and a preparation method thereof. The traditional Chinese medicine preparation for preventing and treating the bacterial diseases of the pigs is prepared from the following raw materials in parts by weight: 10 to 25 parts of dayflower, 10 to 35 parts of dandelions, 10 to 30 parts of cyrtomium fortune, 15 to 35 parts of radix sophorae flavescentis, 3 to 10 parts of morinda officinalis, 20 to 40 parts of radix puerariae, 15 to 35 parts of angelica sinensis, 6 to 12 parts of rhizoma belamcandae and 6 to 18 parts of herba epimedii. The traditional Chinese medicine preparation for preventing and treating the bacterial diseases of the pigs is prepared by nixing various traditional Chinese medicine components; by the adoption of the pure traditional Chinese medicine components, the traditional Chinese medicine preparation does not have toxic and side effect; the traditional Chinese medicine preparation has good prevention and treatment effects on the bacterial diseases of the pigs, particularly swine erysipelas, swine plague, swine escherichia coli and swine dysentery. With readily available raw materials, large drug resource range and convenience for material picking, the traditional Chinese medicine preparation can be popularized.

The invention provides a preparation method of triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus. The obtained vaccine immunization sow can achieve the effects of preventing various viruses by once injection; the blank of lack of the triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus in the market at present is filled. The method uses a specific immunologic adjuvant; after the vaccine immunization, the immunization position cannot generate red swelling and heat generation side reactions; the pig vitality and food intake conditions after the immunization cannot be influenced; the immunization pig immunization effects and the production performance are effectively improved. Compared with the single-antigen vaccine in the prior art, the triple inactivated vaccine has the advantages that the immunization efficiency is identical; the protection rate is higher than 80 percent. Meanwhile, the method provided by the invention has the advantages that the quality stability of the triple inactivated vaccine for swine erysipelas, porcine parvovirus and swine influenza virus can be effectively improved; the defects of operation complexity, great work intensity, easy pollution and the like in the spinner culture process are overcome.

The invention belongs to the fields of microbiology and production of animal medicine, and particularly discloses a selective culture medium for detecting the antigen bacterium content of a swine fever/swine erysipelas/swine plague triple live vaccine, and a preparation method and application thereof. The selective culture medium comprises 29-40mg/ml of TSA (tryptone soybean agar), 3-5% of bovine serum, 20-80mu g/ml of kanamycin and a right amount of sterile double distilled water. The selective culture medium provided by the invention is simple in components and structure, convenient to prepare and easy to operate; and a determination method using the selective culture medium provided by the invention can favorably distinguish a swine erysipelas G4T10 strain and a swine plague EO630 strain in a triple vaccine, can accurately determine the swine erysipelas bacterium content, has favorable repetitiveness and high accuracy, provides a basis for monitoring stable vaccine quality during production, and is also beneficial to vaccine quality monitoring during purchase, thereby ensuring a satisfactory immune effect.

The invention discloses a feed for preventing and treating swine erysipelas and a preparation method for the feed. The feed comprises the following raw materials of barley, wheat, composite enzyme, yam powder, methionine, threonine, vitamin premix and a traditional Chinese medicinal additive. The feed disclosed by the invention has the beneficial effects that the feed contains the traditional Chinese medicinal additive for treating the swine erysipelas, and the main therapeutic principle of the traditional Chinese medicinal additive is to clear away heat and purge pathogenic fire, nourish yin, promote the secretion of saliva or body fluid, regulate qi and blood and regulate spleen and stomach; the traditional Chinese medicinal additive and other feed raw materials are interactive and are matched, so that the feed has an outstanding preventing and treating effect on the swine erysipelas and has the advantages of low side effect, high curing rate, short treating course, less possibility of relapse and the like.

The invention discloses traditional Chinese medicine applied to swine erysipelas and chicken brickpox. The traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20 to 35 parts of ground beetles, 30 to 45 parts of radix codonopsis, 40 to 60 parts of radix isatidis, 40 to 60 parts of radix et rhizoma rhei, 10 to 30 parts of climbing beans, 40 to 60 parts of herba artemisiae scopariae, 5 to 10 parts of scolopendra, 60 to 80 parts of dried roots of radix scutellariae and 8 to 15 parts of toad skin. The traditional Chinese medicine applied to swine erysipelas and chicken brickpox prepared by the invention aims at an acute febrile infectious disease caused by bacillus erysipelatos-suis; the disease has the main features of acute sepsis, hyperpyrexia, (subacute) skin rash, chronic verrucous endocarditis, cutaneous necrosis and chronic multiple non-suppurative arthritis; the effects of fast effect taking, no recurrence and disease condition control are achieved; and in addition, a direct treatment effect is also achieved on chicken brickpox attacked chicken groups with the symptoms of limp bodies, whitewater faeces, transfixed standing, full liquid filling in the crop, empurpled cockscomb, transfixed standing away from the group, falling wings and fluffy feathers.

The invention relates to a swine erysipelas vaccine enrichment medium, which relates to the field of biology. The swine erysipelas vaccine enrichment medium comprises the following raw materials in parts by weight: 5.0-10.0g of peptone, 5.0-10.0g of yeast extract, 5.0-10.0g of beef extract, 5.0-10.0g of beef liver extract, 3.0-5.0g of dipotassium phosphate, 5.0-8.0g of sodium dihydrogen phosphate,5.0-1.0g of glucose, 50-1.0g of Tween 80, 2.0-5.0g of gelatin, and purified water added to 1000ml; a preparation method of swine erysipelas vaccine culture medium comprises the following steps: all raw materials except glucose for the preparation of swine erysipelas vaccine medium are added to purified water, and after being fully dissolved, a sodium hydroxide solution is used to adjust a pH value to 7.8-8.2 and sterilizing is carried out at 116 DEG C for 30 minutes, 12ml of a glucose solution with the final concentration of 50% is added by sterile operation before use, and the materials areuniformly mixed; the preparation of the 50% glucose solution comprises the following steps: weighing 50g of glucose, adding purified water for dissolving, fixing the volume to 100ml, sterilizing at 116 DEG C for 20 minutes, and storing at 2-8 DEG C. The invention relates to an application of a synthetic dry powder culture medium in production of swine erysipelas vaccines. The synthetic dry powderculture medium has the beneficial effects of suitability for high-density culture of swine erysipelas vaccine thalli, selection of commercial raw materials and high bacterial count of cultured bacteria.

The invention belongs to the technical field of research and development of feed additives, and particularly relates to a plant type feed additive for improving immunity of live pigs and a preparation method of the plant type feed additive. The plant type feed additive for improving the immunity of the live pigs is prepared from the following raw materials in parts by weight: 10-25 parts of glabrous greenbrier rhizomes, 10-35 parts of cortex lycii, 10-30 parts of divaricate saposhnikovia roots, 15-35 parts of radix sophorae flavescentis, 3-10 parts of licorice roots, 20-40 parts of radix puerariae, 15-35 parts of radix achyranthis bidentatae, 6-12 parts of rhizoma belamcandae and 6-18 parts of Chinese taxillus herbs. The plant type feed additive for improving the immunity of the live pigs is compounded from various traditional Chinese medicine components, and pure traditional Chinese medicine components are adopted, so that the feed additive is free from toxic and side effects. The feed additive has favorable treatment and prevention effects on bacterial diseases of pigs, particularly swine erysipelas, swine plague, porcine Escherichia coli, red dysentery of pigs, and the like, the raw materials are easy to obtain, the medicinal herb resource is broad, the materials are convenient to take, and the preparation method can be popularized and used.

The invention discloses an application of a traditional Chinese medicine composition used in preparing swine erysipelas treatment medicines. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 11-33 parts of big black grass, 9-27 parts of rosa koreana fruits, 10-30 parts of ocimum gratissimum, 30-90 parts of oroxylum indicumbark, 6-18 parts of pedicularismuscicola maxim, 15-45 parts of cardamine impatiens and 13-39 parts of neolepisorus ovatus. The traditional Chinese medicine composition has the advantages that both symptoms and roots of the swine erysipelas are treated, whole curative effects are high, curative course is short, medicine-resistance is not prone to generation, residuals are low and toxic side effectis small, and thus the traditional Chinese medicine composition has very important economic and social benefits for improving healthy development of a pig raising industry.