12 results about "Pullorum disease" patented technology

The invention relates to the technical field of prevention and control of salmonella pullorum, in particular to novel application of lactobacillus rhamnosus P118. The lactobacillus rhamnosus P118 provided by the invention has any one of the following applications: (1) the infection rate of chicks to salmonella pullorum is reduced, (2) the infection rate of the chicks to salmonella pullorum is reduced, (3) the infection rate of the chicks to salmonella pullorum is reduced, and (4) the infection rate of the chicks to salmonella pullorum is reduced; (2) the egg laying performance of hens infected with salmonella pullorum is improved; (3) reducing the bacterial load in the tissues and organs of the hens; (4) reducing horizontal propagation and vertical propagation of salmonella pullorum; and (5) reducing the risk that salmonella pullorum is vertically spread from hens to offspring through eggs. The antagonistic effect of the lactobacillus rhamnosus P118 on pullorum disease in an in-vivo and in-vitro infection model is systematically evaluated, and it is found that the P118 has a remarkable inhibiting effect on colonization infection of the pullorum disease in chick and laying hen models in the egg laying peak period.

The utility model discloses a portable blood sampling device for detecting pullorum disease, and belongs to the technical field of detection instruments. Comprising a needle cylinder, a blood taking needle is fixed to the middle of the bottom of the needle cylinder, a rubber plug is arranged on the lower portion in the needle cylinder, a push rod is fixed to the middle of the top of the rubber plug, a push handle is fixed to the top of the push rod, a limiting device is arranged above the rubber plug, and a mounting block is arranged on the top of the needle cylinder. A first through hole is formed in the middle of the top of the mounting block, the upper portion of the push rod penetrates through the interior of the first through hole, and a connecting device is arranged at the top of the mounting block. The needle cylinder and the push rod can be conveniently and rapidly disassembled and assembled through the connecting device, the mounting block part and the needle cylinder part can be separated by pressing the fixing block to lift the mounting block upwards, when assembling is needed, the fixing block is located over the fixing hole, the connecting block is inserted into the upper portion of the interior of the needle cylinder, and when the fixing block is opposite to the fixing hole in position, the needle cylinder and the push rod can be assembled. And the fixing blocks are inserted into the fixing holes under the action of the springs.

This invention discloses an SV molecular marker closely associated with the anti-infection phenotype of Salmonella pullorum and its application, belonging to the field of molecular breeding technology. This invention provides an SV molecular marker closely associated with the anti-infection phenotype of Salmonella pullorum. Homozygous Salmonella pullorum susceptible individuals are more resistant than homozygous Salmonella pullorum susceptible individuals, with a 324bp difference shown in SEQ ID NO.1. It also provides a pair of primers for amplifying the SV marker, with sequences shown in SEQ ID NO.2 and SEQ ID NO.3. Using the provided molecular marker and primers, individuals with high resistance to Salmonella pullorum can be identified or selected at an early stage. The detection results are not affected by factors such as the sex, living habits, and environment of the chickens, which can effectively shorten the breeding process, reduce the breeding workload, and improve the stability and reliability of the results. This is of great significance for reducing the economic losses of farmers caused by Salmonella pullorum infection.

The invention relates to the technical field of bacterial detection, in particular to a Salmonella pullorum high-throughput detection method which comprises the following steps: inoculating Salmonella pullorum to a TSB culture medium, culturing to obtain a bacterial liquid, inactivating the bacterial liquid, centrifuging to remove supernatant, dyeing by using a crystal violet ethanol solution, adjusting the concentration of the Salmonella pullorum to 5 * 10 < 7 > CFU / mL to 5 * 10 < 9 > CFU / mL, and carrying out high-throughput detection on the Salmonella pullorum. And preparing to obtain a salmonella gallinarum antigen solution. The method comprises the following steps: diluting serum to be detected, adding into a porous plate, drying and fixing, sealing by using a PBS (Phosphate Buffer Solution), adding a salmonella gallinarum antigen solution, incubating, washing, and observing by naked eyes or measuring by using a microplate reader so as to judge the result. According to the invention, high-flux, low-cost and rapid detection of pullorum disease can be realized, the problem of insufficient accuracy of traditional manual interpretation is solved, and a technical method is provided for identification and diagnosis of pullorum disease.

The invention discloses a method for rapidly extracting and enriching salmonella pullorum nucleic acid based on chitosan modified diatomite. The method comprises the following steps: (1) pretreating diatomite; (2) functional modification of chitosan; and (3) extracting salmonella pullorum nucleic acid. The chitosan-diatomite composite material disclosed by the invention is based on a synergistic mechanism of an electrostatic interaction micropore interception effect, the selectivity is improved by utilizing the cationic characteristic of chitosan, the chitosan is fixed on the surface of diatomite through glutaraldehyde crosslinking, the dissolution loss in an acid environment is avoided, the high specific surface area of the diatomite and the charge adsorption of the chitosan are combined, and the adsorption capacity of the diatomite is improved. A'physical interception + chemical bonding 'dual-mode enrichment mechanism is established, efficient capture of existing nucleic acid is achieved, qPCR detection sensitivity is remarkably improved, the bottleneck problem of a traditional nucleic acid extraction method in complex poultry samples is solved, and the method has the advantages of high detection rate, high anti-interference performance and operation convenience and is suitable for popularization and application. And reliable technical support is provided for rapid molecular diagnosis and epidemiological monitoring of the pullorum disease bacteria.

The invention relates to the technical field of feed additives, in particular to a traditional Chinese medicine extract feed additive for treating pullorum disease and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-15 parts of radix scutellariae, 6-9 parts of rhizoma coptidis, 4-6 parts of fried radix sanguisorbae, 3-5 parts of herba portulacae, 2-3 parts of horseradish tree leaves, 1-2 parts of fructus aurantii immaturus and 1-2 parts of pouzolzia zeylanica. The invention also provides a preparation method of the feed additive, and the prepared feed additive can effectively treat pullorum disease.

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.

The utility model relates to the technical field of veterinary detection equipment, and discloses pullorum disease detection equipment which comprises a box body, a waste barrel placing cabin, a power supply placing cabin, a storage cabin and an LED full-spectrum lamp, and self-locking moving wheels are rotationally connected to four corners of the bottom end of the box body. According to the pullorum disease detection equipment, flexible movement in the chicken house is realized through the self-locking moving wheels, a sandwich structure of the double-layer acrylic plate and the adjustable constant-temperature heating rod ensures that the reaction temperature is stabilized at 20-25 DEG C, and the LED full-spectrum lamp and the white-bottom glass plate are matched to simulate a natural light environment, so that the color development contrast and the judgment accuracy of antigen-antibody reaction are remarkably improved. The waste barrel and the power supply are arranged in a subdivision mode to avoid cross contamination, and a closed cleaning system formed by the guide pipe and the suction ball can achieve boiling water disinfection and waste liquid safe recovery. The linkage design of the auxiliary pulley and the rotating strip simplifies the replacement process of the waste barrel, and the sealed cabin door of the storage cabin guarantees clean storage of consumables. And a rubber plug arranged at the electrolyte notch realizes quick supply and leakage prevention.

The invention relates to the technical field of pullorum disease, in particular to a PCR (Polymerase Chain Reaction) detection kit for quickly identifying salmonella gallinarum with pullorum disease, which comprises a detection system and a shell, and a top cover is hinged to one side of the top of the shell; a loading plate is vertically arranged in the shell in a sliding fit manner, a plurality of loading ports for loading PCR tubes are formed in the loading plate, and loading bags are fixedly connected to the loading ports; an oscillating assembly used for oscillating the loading bag and a limiting assembly used for limiting the position of the loading plate are arranged in the shell. The oscillation assembly comprises an oscillation plate, a controller and a driving part, and the controller is used for controlling the driving part to operate; the oscillating plate is located below the loading bag and is in vertical sliding fit with the inner side wall of the shell; the driving piece is fixedly connected to the inner bottom wall of the shell, and an output shaft of the driving piece is fixedly connected with a stirring wheel. Through the synergistic effect, the oscillation plate can achieve the efficient oscillation effect, it is ensured that reactants are evenly distributed in a PCR tube, and the uniformity and efficiency of the PCR reaction are improved.

The invention discloses a salmonella longtail bacteriophage and application thereof, the bacteriophage is salmonella bacteriophage YZA15 and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242878, the preservation date is December 23, 2024, and the preservation address is Wuhan. The salmonella bacteriophage YZA15 disclosed by the invention is good in stability, has no toxic drug-resistant genes, has splitting activity on main epidemic serotypes (mouse typhoid, enteritis and pullorum disease) of salmonella, is relatively specific in host range, short in incubation period and high in filial generation bacteriophage outbreak amount, can be applied to links of livestock breeding, slaughtering, processing and breeding, and has a broad application prospect. And a safe and efficient biological antibacterial product is provided for livestock and poultry environments and products thereof.

The invention discloses a novel salmonella pullorum SP1910 strain, a pullorum disease antigen prepared from the novel salmonella pullorum SP1910 strain and a preparation method and application of the antigen, the strain has better stability and specificity, the detection effect of the antigen prepared from the strain on pullorum disease is superior to that of a commercial antigen, the pullorum disease can be efficiently detected, positive chickens are eliminated, and the detection cost is reduced. Chicken flocks are purified, infection of pullorum disease is reduced, and breeding benefits are improved.

The invention discloses an RPA primer group and a kit for rapidly detecting salmonella pullorum based on RPA-CRISPR / Cas12a, and belongs to the technical field of salmonella pullorum visual rapid detection. A specific target gene ipaj in a salmonella pullorum genome is taken as a target spot, an RPA primer pair and a specific crRNA sequence are designed and optimized, the salmonella pullorum is detected by combining an RPA isothermal amplification method and a CRISPR / Cas12a detection method, a detection result can be interpreted by naked eyes under blue light, and the detection result is accurate. The kit has the characteristics of high sensitivity, strong specificity, accurate result, short time consumption, simplicity and convenience in operation and high practicability, and is suitable for rapid screening or detection of the pullorum disease on site.