137 results about "Pullorum disease" patented technology

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The invention discloses feed for chicks. The feed is prepared from following raw materials by weight percentage: 350-450 of corn flour, 70-90 of wheat bran, 70-80 of linseed meal, 30-40 of flour weevil powder, 20-30 of fish meal, 25-35 of rice chaff, 15-20 of soybean curb residue, 35-40 of sugarcane leaves, 20-30 of aloe dry powder, 10-20 of bone meal, 2-3 of edible salt, 15-20 of a traditional Chinese additive and 4-5 of phagostimulant. The traditional Chinese additive comprises 20-22 of golden cypress, 15-20 of herba pteridis multifidae, 15-18 of the root of Chinese pulsatilla, 12-15 of scutellaria baicalensis, 8-15 of gallnut and 5-8 of cassia twig. Natural medicine components in the traditional Chinese medicine additive can prevent and treat chick pullorum disease and coccidiosis, thus reducing the death rate of the chicks and increasing the breeding ratio. In addition, by using the feed having the formula, the chicks have increased food intake and rapid growth and development. The chicks fed with the feed provided by the invention has more significant gained weight than chicks fed with traditional feed.

The invention discloses a traditional Chinese veterinary medicine used for treating livestock, poultry and fish diseases. The effective components of the medicine are pink plumepoppy herb and polygonum flaccidum. One or more materials selected from baikal skullcap root, common andrographis herb, and houttuynia cordata can be contained in the medicine. The medicine provided by the invention has advantages of fast effect, good effect, no medicine residue, no obvious side-effect, and no drug resistance. When the medicine is used for treating young pig diarrhea, a recovery rate is no less than 91.81%. When the medicine is used for treating pullorum disease, a recovery rate is no less than 92.22%. When the medicine is used for treating dairy cow mammitis, a recovery rate is no less than 87.87%. When the medicine is used for treating fish enteritis, a recovery rate is no less than 92.61%.

The invention provides traditional Chinese medicine for prevention and treatment of pullorum diseases, which comprises coptis chinensis, radix sophorae flavescentis, pulsatilla chinensis, rhizoma anemarrhenae, selfheal, honeysuckle, radix scrophulariae, codonopsis pilosula, rhizoma atractylodis macrocephalae, lotus seeds, dark plum, granatum and semen coicis. The traditional Chinese medicine has the advantages of high efficacy, fewer toxic and side effects, no residues, no resistance to drugs, no environmental pollution, capability of improving the body resistance, short treatment course, rapid effects and wide scope of promotion and application in prevention and treatment of chicken suffering from pullorum diseases.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The invention discloses an aerosol for preventing and controlling pullorum disease and a preparation method thereof. The aerosol is prepared from the following raw materials by weight/volume: 50 to 200 grams of golden thread, 50 to 200 grams of amur corktree bark, 50 to 100 grams of rhubarb, 50 to 200 grams of baical skullcap root, 50 to 200 grams of indigowoad root, 50 to 100 grams of liquoric root, 10 to 80 grams of propellant, 50 to 150 milliliters of propylene glycol, 100 to 350 milliliters of ethanol and the balance of water. The preparation method comprises the following steps of: extracting active ingredients of the golden thread, the amur corktree bark, the rhubarb, the indigowoad root and the liquoric root and extracting active ingredients of the baical skullcap root; and mixing the extracts, adjusting pH value, filtering, adding active carbon to adsorb impurities, leaching, adding the propylene glycol, the ethanol and the water, adjusting the pH value to an acid state, adding the water until the volume is 1,000 milliliters, mixing uniformly, packaging and filling after a product is up to the standard and introducing the propellant after covering so as to obtain the aerosol. The product has the effects of clearing away heat, reducing fire, diminishing inflammation and relieving dysentery and has the advantages of convenient administration, safety, nontoxicity, no medicament residue, difficult medicament tolerance and high efficiency.

The invention discloses powder for treatment of chicken colibacillosis and a preparation method thereof. The powder is mainly composed of the following components by weight: 40 parts of pulsatilla chinensis, 30 parts of dandelion, 20 parts of scutellaria baicalensis, 25 parts of honeysuckle, 10 parts of phellodendron, 30 parts of sophora flavescens, 15 parts of gentian, 15 parts of bighead atractylodes rhizome, and 10 parts of licorice. The Chinese veterinary medicinal composition for prevention and treatment of chicken colibacillosis provided by the invention has a significant curative effect on pullorum disease and a remarkable prevention effect, has good popularization and application prospects, and can promote the sound development of pollution-free cultivation and no residual green meat food processing industry.

The invention relates to an oral solution which is made from traditional Chinese medicines and used for treating digestive tract diseases of livestock and poultry. The oral solution is made from three medicinal herbs, namely, ash bark, phellodendron bark and Chinese pulsatilla root. The oral solution is suitable for treating the digestive tract infection of the livestock and poultry, such as avian colibacillosis, piglet diarrhea, pullorum disease and the like. And at the same time, in consideration of the special physiological structure of poultry such as short avian reproductive tract and inability to digest wood fiber, the oral solution is provided to facilitate quick dispersion in feed water. The oral solution is administered in drinking water of the diseased livestock and poultry, thus being convenient and good to absorb, and achieving the purpose of taking effect quickly.

The invention belongs to the field of biotechnology detection, and particularly relates to a PCR detecting kit for fast identifying pullorum disease and chicken salmonella typhosa. The kit comprises flhB gene detection primers, and the flhB gene detection primers comprise a forward primer with the nucleotide sequence shown as SEQ ID NO.1 and a reverse primer with the nucleotide sequence shown as SEQ ID NO.2. The kit can fast identify the pullorum disease/chicken salmonella typhosa at high flux, and can be adopted as an auxiliary method for salmonella traditional serological typing, and a simple, fast and good-repeatability novel method is provided for monitoring and laboratory diagnosis of pullorum disease/chicken salmonella typhosa.

The invention discloses an efficient method for breeding and freely raising high-quality Jing Yuan chickens and belongs to the cultivation technical field. The efficient method for breeding and freely raising high-quality Jing Yuan chickens comprises following steps: purebred seed selecting, purebred propagating, hatching egg disinfecting, hatching, brooding, free raising management and disease preventing and controlling. The breeding time is shortened, the breeding precision, the egg fertilization rate, the hatching rate and the survival rate of broods are increased and the hatching period of chicks is shortened by using the method of artificial breed protection and selection and artificial insemination of the Jing Yuan chicken of the present invention; at the same time, the method of the present invention adopts Chinese herbal medicine to control chicken diseases, improve the resistance of chickens, reduce the incidence of pullorum disease, mycosis and coccidiosis; by means of the method of ecological freely raising Jing Yuan chicken, the output of eggs is increased, the oviposition time is advanced, the slaughtering time is shortened, the body weights of hens and cocks when slaughtering are substantially increased so that the chicken is delicious, there is no residual medicine and the chicken quality of Jing Yuan chicken is increased.

The invention discloses a salmonella bacteriophage and an application thereof in medicines for preventing and treating salmonella infection diseases. The bacteriophage is named as SPP11 and is preserved in China General Microbiological Culture Collection Center, wherein the preservation date is Nov.04,2019, and the preservation number is CGMCC No.18868. The bacteriophage SPP11 with a strong cracking spectrum has a strong cracking effect on salmonella, can effectively prevent and control salmonella diseases in poultry farms, reduce positivity of the pullorum disease antibody of chickens, reducediarrhea of chickens caused by salmonella enteritis, and can also be used for salmonella sterilization of feeding environment, feed, drinking water and the like. In addition, the bacteriophage is easy for industrial production, and medicines or disinfectants prepared by the bacteriophage not only have reduced cost, but also have the advantages of environmental friendliness.

The invention discloses a novel antibacterial antiviral feed additive for a livestock and poultry breeding industry. The novel antibacterial antiviral feed additive is composed of plume poppy, lightyellow sophora root, macleaya microcarpa, yucconin, tea saponin, Origanum vulgare L., narcissus seed and pagodatree flower bud powder and also comprises a hydrophilic aluminosilicate as a carrier. The novel antibacterial antiviral feed additive has the advantages that specific active components of natural plants can directly or indirectly kill viruses, have strong antibacterial effects and a wide antibacterial spectrum and compared with the common drugs such as berberine hydrochloride, penicillin and aureomycin, the specific active components have stronger activities of resisting some bacteria; in feeding, antibiotic drugs are avoided and mycotoxins are reduced and good effects of treating and preventing avian pasteurellosis, escherichia coli, bacillus rhusiopathiae suis, cholera fowl, white scour of piglets, and pullorum disease are obtained; and the novel antibacterial antiviral feed additive can resist stress and coccidium and remove heavy metals and drug residues.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor, adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled water and refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention provides application of salmonella pullorum secreted protein SpiC. The salmonella pullorum secreted protein SpiC can be taken as a biological marker for diagnosis when salmonella infects a host, and is used for preparing materials for pullorum disease immunodetection or materials for diagnosis. According to the invention, the protein is further utilized to establish materials applied to pullorum disease ELISA (enzyme-linked immuno sorbent assay) of a SpiC antibody generated in the host, and a pullorum disease ELISA method is correspondingly given. The protein-based ELISA method for detecting the SpiC antibody generated in the host is good in specificity and high in flexibility, and can be applied to the evaluation of organism immune states and the research relevant to infectious disease diagnosis.

The invention relates to a low-cost matched breeding method for white feather broilers. According to the method, recessive white Plymouth rocks serve as a specialized strain female parent paternal line, high-yield laying hens serve as a specialized strain female parent maternal line, the recessive white Plymouth rocks and the high-yield laying hens are hybridized to generate female parents, and aCornish variety serves as a male parent to be hybridized with the female parents to generate a commercial generation of a specialized strain. Leukemia and pullorum disease full-group general test is preferably carried out on specialized strain progenitor chicken flocks, regular spot test is carried out on the parents, and leukemia individuals and pullorum disease positive individuals are eliminated. Pure line breeding is preferably carried out on the specialized strain paternal line, the female parent paternal line and the female parent maternal line. The breeding cost of the commercial generation is reduced, the growth speed of the commercial generation can be ensured, and the production cost is reduced.

The invention provides a Chinese herbal medicine compound preparation for treating pullorum disease with an obvious effect. The preparation is prepared from the following raw materials in parts by weight: 15-25 parts of astragalus, 35-45 parts of scutellaria baicalensis, 25-35 parts of golden cypress, 25-35 parts of the root of Chinese pulsatilla, 25-35 parts of fructus forsythiae, 15-25 parts of cape jasmine, 25-35 parts of radix paeoniae alba, 15-25 parts of pericarpium citri reticulatae, 55-65 parts of rhizome atractylodis, 10-20 parts of smoked pulm, 15-25 parts of herba portulacae, 25-35 parts of folium isatidis, 5-15 parts of radix sophorae flacescentis, 15-25 parts of the bark of ash, 25-35 parts of wolfiporia extensa. The preparation is prepared by carrying out superfine grinding on the raw medicinal materials until the particle size is 30-90 microns, weighing in a mixture ratio and uniformly mixing. The Chinese herbal medicine compound preparation provided by the invention has the efficacies of removing heat for detoxication and eliminating dampness for preventing dysentery, and mainly acts on endoretention of damp heat and pullorum disease; a micronization technology is adopted, so that medicine waste is reduced, energy is saved, and environment is protected; and dosage is reduced, and treatment cost is reduced.

The invention relates to a Chinese veterinary drug used for curing pullorum disease of livestock and a preparation method thereof; the preparation method is characterized by comprising according to the weight parts: 5-15 of atractylodes, 3-5 of coptis, 3-5 of phellodendron, 1-3 of phellodendron and 20-60 of carrier. The Chinese veterinary drug used for curing the pullorum disease of the livestock is prepared by the steps as follows: the functional components are sequentially crushed, decocted, concentrated, dried and crushed as powder; the powder is subsequently mixed uniformly with the carrier. The Chinese veterinary drug has extremely good curative effect and quick effect on coli disease, pullorum disease and Leucocytozoon caulleryi infection, the treatment is not easy to recur, no drug remains and the control rate reaches more than 95%.

The invention discloses a livestock seminal fluid disinfectant, a preparation method thereof and an application. The disinfectant consists of the following components by weight percent: 1.0 to 2.0 percent of radix isatidis extract, 1.0 to 2.0 percent of melia azedarach extract, 1.0 to 2.0 percent of sanguisorba officinalis extract, 1.0 to 2.0 percent of folium isatidis extract, 1.0 to 2.0 percent of aloe extract, 2.0 to 3.0 percent of glucose/ fructose, 3.0 to 5.0 percent of glycerinum, 2.0 to 3.0 percent of sodium citrate, 0.2 to 0.3 percent of vitamin, and the balance of distilled water, wherein the vitamin is one or a combination of more than two of vitamin C, vitamin A and vitamin E. The disinfectant can inhibit or kill hog cholera virus, porcine circovirus, avian leukosis and pullorum disease salmonella pathogen. A main formula of the seminal fluid disinfectant is a pure traditional Chinese herba preparation, so that the disinfectant has low toxicity, no residue and good safety; and by virtue of the test for the negative influence on the activity of sperms, the disinfectant is small in toxicity and is safe and reliable.

The invention discloses an inert carrier indirect agglutination test detection system and application thereof. The system comprises inert carrier bacteria S9 and a complex S9-P which is shown and expressed on the surface of the inert carrier bacteria S9 and carries a P-antibody factor. The system only carries the P-antibody factor, is single in component and specific in targeting, generates macroscopic positive particle agglutination reaction with whole blood or serum of chicken infected by pullorum disease and salmonella typhimurium under a certain concentration condition, and does not generate non-specific cross agglutination reaction with chicken-derived serum and whole blood of different backgrounds infected by non-pullorum disease and salmonella typhimurium. The S9-P is based on a glass plate agglutination reaction operation platform. The S9-P is simple and convenient to operate, sensitive and rapid in reaction, macroscopic in agglutination reaction particles and clear and easy inresult judgment, tests and result judgment are completed within two minutes, and the S9-P is suitable for targeted specific detection of pullorum disease and salmonella gallinarum infection in chicken flocks and has a good application prospect in on-site monitoring and diagnosis of the chicken flocks.

The invention relates to a traditional Chinese medicine composition for treating a pullorum disease. The traditional Chinese medicine composition comprises the following components in parts by weight: 10 to 15 parts of realgar, 20 to 30 parts of agastache rugosus, 15 to 20 parts of cortex fraxini, 10 to 20 parts of talc, 30 to 40 parts of fructus chebulae, 20 to 40 parts of cortex phellodendri, 40 to 60 parts of anemone chinensis and 20 to 30 parts of meadowrue root and rhizome. The traditional Chinese medicine composition for treating the pullorum disease provided by the invention is simple in preparation method and low in cost; furthermore, the effective components of the traditional Chinese medicine composition are liable to be absorbed by the poultry farming animals, the time is short and the efficiency is high; and the traditional Chinese medicine composition has the effects on protecting gastrointestinal mucosa, relieving vomiting, stopping diarrhea and dysentery, and improving the poultry immunity, and avoids the medicinal residues, and is convenient to use and low in cost.

The invention relates to a pure Chinese medicinal composition for treating chicken colibacillosis and pullorum disease. The composition is prepared by mixing the following components in part by weight: 15 to 25 parts of dangshen, 15 to 35 parts of astragalus, 15 to 25 parts of coptis root, 15 to 25 parts of weeping forsythia, 15 to 25 parts of red-rooted salvia root, 15 to 25 parts of common anemarrhena, 10 to 30 parts of red paeony root, 15 to 25 parts of giant knotweed, 15 to 35 parts of garden burnet root, 15 to 35 parts of malt, 15 to 40 parts of tuckahoe and 15 to 25 parts of liquorice root. The pure Chinese medicinal composition is reasonable in formula, is easy to prepare, does not have any toxic or side effect, is subjected to superfine grinding, is high in bioavailability, has a very obvious curative effect and is clinically proven to have a special effect of treating chicken colibacillosis and pullorum disease, and pollution, medicine residue and medicine resistance are avoided.

The invention discloses a special feed for black-bone chickens and a preparation method of the special feed. The special feed for the black-bone chickens is prepared by the following formula in parts by weight: 24-26 parts of sorghum flour, 20-23 parts of corn flour, 16-18 parts of all-fish meal, 15-17 parts of sheep bone meal, 13-16 parts of wheat bran, 12-15 parts of hay leaves, 10-13 parts of bean cakes, 1-3 parts of fine sand, 4-6 parts of methionine, 3-5 parts of tryptophan, 2-5 parts of vitamin D, 2-4 parts of vitamin E, 1-2 parts of chloromycetin, 2-3 parts of table salt and 30-40 parts of water. The feed is balanced in nutrition and scientific and simple in ratio; chloromycetin in the formula can be used for controlling the pullorum disease of black-bone chickens; the fine sand is capable of improving the digestion function of the black-bone chickens, so that the feed intake is improved, and the growth of the black-bone chickens is effectively promoted.