157 results about "Salmonella escanaba" patented technology

Attenuated immunogenic bacteria having an RpoS+ phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS+ phenotype and methods therefor are disclosed. The Salmonella have in addition to an RpoS+ phenotype, an inactivating mutation in one or more genes which render the microbe attenuated, and a recombinant gene capable of expressing a desired protein. The Salmonella are attenuated and have high immunogenicity so that they can be used in vaccines and as delivery vehicles for genes and gene products. Also disclosed are methods for preparing the vaccine delivery vehicles.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The invention relates to a culture medium for simultaneous composite enrichment of five kinds of food-borne pathogenic bacteria, namely Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella, and a preparation method for the culture medium. Food-borne pathogenic bacteria are a significant reason to cause food positioning, so the rapid and accurate detection of the food-borne pathogenic bacteria has an important practical significance for preventing and controlling food safety incidents. The culture medium is characterized by comprising the following components: 10.0 g of peptone, 10.0 g of sodium chloride, 9.0 g of disodium hydrogen phosphate, 1.5 g of monopotassium phosphate, 0.1 g of cholate, 0.1 mg of potassium tellurite, 1.0 g of lithium chloride, 3.0 g of glucose, 2.0 g of mannitol, 2.5 g of sodium pyruvate, 1.0 g of aesculin and 1,000 mL of distilled water, wherein the pH value is 7.1 to 7.5. The culture medium can simultaneously enrich five kinds of target pathogenic bacteria, can be used for separation and identification of target bacteria, can also be used for the molecular detection of multiple pathogenic bacteria on the same detection platform, provides technical support for a method for rapidly detecting five kinds of pathogenic bacteria in food, and meets the requirement of simultaneous detection of five kinds of food-borne pathogenic bacteria.

The invention relates to the field of microbes and provides a novel salmonella phage and a composition, a preparation method and application thereof. The novelsalmonella phage is Myoviridaesp. BP-66 with the preservation numberCCTCC NO: M2015146,Myoviridaesp. BP-63 with preservation number CCTCC NO: M2015145, or Chilikevirussp. BP-12 with the preservation number CCTCCNO: M2015141. The novel phage is a strict virulent phage, has high toxicity to host bacteria, has a wider host range and still has high toxicity to the host bacteria at low concentration. The DNA of the phage cannot encode protein possibly causing potential health risks. The novel phage stably survives in a culture solution at room temperature and survive for more than 6 months at the temperature below 4 DEG C. In addition, the phage can be proliferated on pathogenic bacterial hosts, and large-scale industrial production can be achieved. The salmonella phage can provide excellent strain resources for application of phage therapy.

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

The invention provides a lactobacillus plantarum AB-3 strain having an activity of resisting phytophthora melonis kalsura, of which the accession number is CGMCC No.9655. The lactobacillus plantarum AB-3 has effective inhibition properties on escherichia coli, salmonella typhimurium and shigella flexneri, has effective inhibition properties on the phytophthora melonis kalsura, fusarium oxysporum, penicillium roqueforti and glomerella cingulata and is excellent in thermostability. A fermentation supernate of the strain shows a significantly increased bacteria inhibition rate after treatment at 100 DEG C and is enhanced in bacteria inhibition activity through enzymolysis by a neutral protease. The fermentation supernate of the strain has a storage resistance property and is excellent in bacteria inhibition activity even after normal storage for 20 days.

The invention discloses a Lactobacillus, paracasei subsp. paracasei strain, a bacteriostatic composition and applications thereof. The preservation number of the SG96 strain microorganism is CGMCC2697. The strain can inhibit the growth of pathogenic bacteria of Escherichia coli, Salmonella typhimurium and the like, and has bacteria killing effect. The invention also discloses a bacteriostatic composition containing the Lactobacillus, paracasei subsp. paracasei strain SG96. The composition can be prepared into animal water-feeding additives, animal fodder additives, animal and human medical compositions, food additives, beverage additives, foods, beverages, health foods, and the like.

The invention belongs to the technical field of escherichia coli phage, and particularly relates to a broad-spectrum escherichia coli phage capable of cracking four bacteria and an application of the broad-spectrum escherichia coli phage to sterilization and bacterium prevention. The invention mainly discloses an escherichia coli phage EC35P1 (Escherichia coli phase EC35P1), and the preservation number of the escherichia coli phage EC35P1 is CCTCC M 2020438. The phage is a virulent phage separated from the nature, tests prove that the phage has no toxic effect on normal microbial flora, and DNA of the phage cannot encode virulence genes and is high in stability. The phage is wide in host range, can be used for cracking escherichia coli, shigella, salmonella and enterobacter cloacae, and large-scale industrial production can be realized. The phage provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect.

The invention relates to a microbial detection device and a detection method of meat of livestock and poultry. The salmonella and Escherichia coli O157 multiple PCR rapid detection kit of the meat of livestock and poultry includes: 10 multiplied by PCR buffer, 1.0 to 3.0mmol / L of MgCl2, 240 Mumol / L of dNTP each, 200nmol / L of salmonella primer, 200nmol / L of Escherichia coli O157 primer and 1.3 to 3.0U of Taq enzyme. The microbial detection device has better specificity, which can complete the simultaneous detection of the salmonella and the Escherichia coli O157 within 8 to 9h; compared with the conventional bacteriology, the diagnosis is rapid, convenient and economical, thus providing a powerful technical means for the diagnosis of pathogen in a food sample and having good application prospect.

The invention discloses a strain of lactobacillus salivarius ZLp4b and its application in preventing and treating diarrhea of young animals. The strain has broad-spectrum bacteriostasis and can inhibit the growth of various pathogenic bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus and PasteureUa multocida, etc. In addition, the strain has good in-vitro probiotic characteristics, including high gastrointestinal tolerance, high temperature tolerance and adhesion. As the lactobacillus salivarius of the invention is applied to feeding of mice and diarrhea piglets, lambs and calves, the secretion of immunoglobulin and cytokines in the serum of mice can be promoted, and the diarrhea symptoms of young animals can be cured or obviously alleviated.

The present invention relates to one kind of gene chip for detecting six kinds of diarrhea pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The oligonucleotide probe includes DNA or cDNA segments selected from nucleotide sequences corresponding to the genomes of Shigella, haemorrhagic colibacillus, invasive colibacillus, Vibrio parahaemolyticus, Vibrio cholreae and salmonella. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency and high detection accuracy.

The invention discloses a method for fast identifying clinical pathogens (such as staphylococcus aureus, salmonellae and escherichia coli). The method includes the following steps that near infrared spectrograms of staphylococcus aureus, salmonellae and escherichia coli samples are collected; PCA dimension reduction processing is carried out on the near infrared spectrograms of the staphylococcus aureus, salmonellae and escherichia coli samples; a Fisher discrimination function is set up for classification. The adopted principal component analysis method in combination with Fisher discriminance is introduced to identify the common pathogens, the types of the pathogens can be fast identified, and the method has the advantages of being high in accuracy and easy to operate, making a great contribution to follow-up clinical strain identification and having good application prospects.

The invention discloses a multiple rapid detection method for three food borne pathogenic bacteria, and detection primer sets and a detection kit thereof. An upstream primer and a downstream primer of a rapid detection primer set for Salmonella have sequences as represented by SEQ No.1 and SEQ No.2, respectively; an upstream primer and a downstream primer of a rapid detection primer set for Shigella have sequences as represented by SEQ No.3 and SEQ No.4, respectively; an upstream primer and a downstream primer of a rapid detection primer set for o Staphylococcus aureus have sequences as represented by SEQ No.5 and SEQ No.6, respectively. Bacterial genome DNA extracted from a sample to be tested is subjected to a LAMP reaction with the primer sets in a same reaction system, and the resultant of the reaction is identified to determine whether the sample contains Salmonella, Shigella or Staphylococcus aureus. Every primer set provided in the invention has strong specificity and can accurately detect genome DNAs of Salmonella, Shigella and Staphylococcus aureus in a same reaction system.

The invention discloses a lactobacillus plantarum SWUN5815 for high-yield bacteriocin and an application of the lactobacillus plantarum SWUN5815. The strain is preserved at the China Center for Type Culture Collection (CCCTCC) on August 2, 2016 and the preservation number is CCTCC NO: M2016419; and the strain is separated from yak dung. The bacteria generated by the strain can be degraded by protease; the strain has a very high inhibition effect on salmonella and escherichia coli and has good acid resistance and relatively high cholate tolerance; the survival rate after 3h in artificial gastric juice of which the pH is 3.0 is 93.36+ / -4.06%; the strain can slowly grow in 1.0% cholate; and the growth efficiency reaches 15.26+ / -1.19% of that of the strain which is cultivated under the condition free of the cholate. The bacteriocin is capable of effectively inhibiting growth of salmonella, escherichia coli and staphylococcus aureus. Therefore, the lactobacillus plantarum SWUN5815 for the high-yield bacteriocin has the potential of being applied to yak intestinal tracts as a microecological preparation.

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer group for avian pathogenic escherichia coli, pasteurella multocida, proteus mirabilis, pseudomonas aeruginosa, salmonella andstaphylococcus aureus, a multiplex PCR detection kit comprising the primer group, and a multiplex PCR detection method using the primer group. The primer group comprises primer pairs PhoA-F and PhoA-Rfor specifically amplifying a gene phoA of the avian escherichia coli, primer pairs KMT-F and KMT-R for specifically amplifying a gene KMT1 of the pasteurella multocida, primer pairs AtpD-F and AtpD-R for specifically amplifying a gene AtpD of the proteus mirabilis, primer pairs PETA-F and PETA-R for specifically amplifying a gene PETA of the pseudomonas aeruginosa, primer pairs InvA-F and InvA-Rfor specifically amplifying a gene InvA of the salmonella, and prier pairs NuC-F and NuC-R for specifically amplifying a gene nuc of the staphylococcus aureus.

The invention discloses a salmonella phage RDP-NSA-19050 with a wide lysis spectrum, wherein the preservation number is CGMCC No.21415, the gene length is 87591 bp; after sequencing is carried out on whole genes, it is found that the salmonella phage RDP-NSA-19050 does not have virulence genes and lyogenic genes, and the salmonella phage RDP-NSA-19050 has a good lysis spectrum. The salmonella bacteriophage RDP-NSA-19050 provided by the invention can be used for cracking a plurality of pathogenic bacteria such as salmonella steulani, salmonella gallinarum, salmonella typhimurium, salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella enteritidis and salmonella kkinensis; the salmonella bacteriophage RDP-NSA-19050 has the characteristics of wide splitting spectrum and high splitting rate, can be independently used, can also be compounded with bacteriophages of other escherichia coli and salmonella and probiotics for use, and can effectively reduce the death rate of chicken infected with salmonella.

The present invention relates to novel peptide compounds derived from flagellin originating from Salmonella enterica that exhibit an in vivo immune adjuvant activity.

The invention relates to a detection assembly for detecting bacteria based on a bacteria fluorescent staining technique. A single antibody is utilized for detecting thalli which are stained by fluorescence; an escherichia coli O157:H7 antibody, a listeria monocytogenes antibody and a salmonella typhimurium antibody are sprayed on a test strip, and the test strip acts an immunochromatography test strip with three detection lines (T1, T2 and T3 lines). The detection assembly for detecting bacteria has the advantages that fluorochrome is used for pre-staining thalli, and the respective specific antibody is utilized again and is sprayed onto the T lines of the test strip so as to capture target thalli in a mixed sample, so that the thalli in the sample are detected rapidly and specifically; the detection assembly for detecting bacteria can be used for simultaneously detecting the food-borne pathogenic bacteria including the escherichia coli O157:H7 antibody, the listeria monocytogenes antibody and the salmonella typhimurium.

Belonging to the field of animal biomedical engineering, the invention specifically discloses a preparation method of a bacteriophage lyase capable of lysing escherichia coli and salmonella. Through primer design, a lyase gene of bacteriophage ECGD1 is successfully amplified by PCR, and is transferred into an expression system to obtain a recombinant strain. IPTG induction is carried out on the recombinant strain to obtain expression, then the strain is lysed to obtain a soluble recombinant lyase of bacteriophage ECGD1, the recombinant lyase can lyse a plurality of escherichia coli and salmonella, i.e. having a wide lysis spectrum. In addition, the synergistic effect result of the recombinant lyase and polymyxin B shows that the existence of the recombinant lyase lowers the minimum inhibitory concentration of polymyxin B, i.e. the two have a synergistic bacteriostatic effect. Identification on the lysis activity of the recombinant lyase and identification on the wide lysis spectrum thereof reveal that the recombinant lyase of the bacteriophage ECGD1 has potential application value in prevention and control of escherichia coli and salmonella infection.

The present invention relates to a compound vitelline antibody (anti-BAIgY) with antibody activity for resisting salmonella gallinarum, bacterium multocidum and chicken colibacillus, method for preparing said antibody and application of said antibody in preparation of medicine or feed additive for curing relatied diseases which can be infected by above-mentioned bacteria. The preparation method of said compound vitelline antibody includes the following steps: preparing compound antigen of the above-mentioned bacteria, immunizing health hen with said antigen, collecting egg and extracting anti-BA IgY from egg.

The invention belongs to the field of microbiological detection and discloses a method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in food simultaneously and rapidly. A special primer is designed aiming at salmonella typhimurium, salmonella paratyphi b and salmonella typhi, hydrazoic brominated c ingot processing is adopted to remove interference of dead bacteria, and finally deoxyribonucleic acids (DNAs) are extracted to perform multiplex polymerase chain reaction. Compared with a classical bacterium separating and identifying method and a serologic typing method, the method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in the food simultaneously and rapidly is rapid and accurate.

The invention provides a bacteriophage composition, a bacteriostatic agent and application thereof, and belongs to the technical field of bioengineering. The bacteriophage composition comprises a clostridium perfringens bacteriophage (CCTCC NO: M2020047), a salmonella bacteriophage (CCTCC NO: M2020710), an escherichia coli bacteriophage (CCTCC NO: M2020711) and a shigella flexneri bacteriophage (CCTCC NO: M209029). The bacteriophage composition has a strong lysis effect on clostridium perfringens, salmonella, escherichia coli and shigella flexneri, and can be used for inhibiting and / or eliminating clostridium perfringens, salmonella, escherichia coli and shigella flexneri; by inhibiting the overgrowth of clostridium perfringens, salmonella, escherichia coli and shigella flexneri, the occurrence of intestinal diseases of animals is effectively prevented and controlled; a safe bacteriophage antibacterial product source without toxic and side effects is provided for preventing and controlling animal intestinal diseases caused by clostridium perfringens, salmonella, escherichia coli and shigella flexneri.

The invention provides a pediococcus pentosaceus NHB-PpA9601. The pediococcus pentosaceus NHB-PpA9601 is separated from intestinal tracts of white feather broilers, and is preserved in China General Microbiological Culture Collection Center (CGMCC for short, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101), the preservation number is CGMCC NO.22646, and the preservation date is May 31, 2021. The pediococcus pentosaceus NHB-PpA9601 has the advantages that the obvious probiotic property is realized, the growth and reproduction of pathogenic bacteria such as intestinal pathogenic escherichia coli, staphylococcus aureus, salmonella suis, salmonella choleraesuis, salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmonella, shigella, bacillus perfringens, proteus penneri, aeromonas hydrophila and vibrio parahaemolyticus can be effectively inhibited; the pediococcus pentosaceus NHB-PpA9601 can be used for producing a wet-based fermented feed, and can be used for effectively improving intestinal health and increasing economic benefits.

The invention relates to the field of molecular biology and production of animal medicine, in particular to a multi-PCR (Polymerase Chain Reaction) specific primer for detecting bacterial enteritis pathogens, such as escherichia coli, salmonellae and clostridium perfringens, of a pig; nucleotide sequences are shown as SEQ ID NO:1 to SEQ ID NO:6; the specificity, the repeatability and the sensitivity are high. The invention also discloses a method which is established on the basis of the primer and is used for detecting the bacterial enteritis pathogens, such as the escherichia coli, the salmonellae and the clostridium perfringens, of the pig; the enteritis of the pig which is caused by single infection or mixed infection of the escherichia coli, the salmonellae and the clostridium perfringens can be distinguished quickly; the primer can be used to identify and diagnose quickly and effectively, and is beneficial to formulating targeted prevention and control measures to perform immune prevention and control on the bacterial enteritis pathogens of the pig in clinical parturition and ensure smooth parturition of the raised pig.

The invention discloses a lipid type antibacterial composition. The lipid type antibacterial composition is extracted from black soldier fly larvae and contains 18.0 to 22.0 percent of glyceryl oleate, 7.7 to 10.0 percent of lauric acid and 15.5 to 18.0 percent of myristic acid. The lipid type antibacterial composition belongs to the field of biology; the composition has an excellent antibacterialeffect and at least can have a specific antibacterial effect on part of escherichia coli and enteritis type salmonella; meanwhile, the invention further discloses an extraction method, a detection method and application of the composition.

The invention relates to a Lactobacillus plantarum strain Y388 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the Lactobacillus plantarum strain Y388 is CGMCC No. 21750. The Lactobacillus plantarum strain Y388 provided by the invention can be used for remarkably producing the exopolysaccharide, is sensitive to 14 common antibiotics, has a relatively strong inhibition effect on escherichia coli, staphylococcus aureus and salmonella, and is a safe, healthy and nutritional probiotic capable of producing the exopolysaccharide at high yield.

The invention discloses a detecting kit for five kinds of food-borne pathogenic bacteria and a detection method, and belongs to the field of molecular biology and food safety detection. The kit is mainly prepared from primer pairs of salmonella, primer pairs of staphylococcus aureus, primer pairs of escherichia coli O157:H7, primer pairs of vibrio parahaemolyticus, primer pairs of listeria monocytogenes, Taq DNA polymerase, 10*polymerase chain reaction buffer, deoxy-ribonucleoside triphosphate and MgCl2. In the detection process, a to-be-detected sample polymerase chain reaction template is prepared and added to the kit for polymerase chain reaction amplification, and then amplification products are subjected to electrophoresis and positive control. According to the detection method, the five kinds of pathogenic bacteria in food can be detected at the same time, and detection sensitivity is high. The detection process is simple, and popularization is easy.

The invention provides a construction and fermentation method of an artificial strain with high yield of fengycin. The 4'-phosphoric acid pantetheinyl transferase gene sfp from bacillus amyloliquefaciens FZB42, a multi-effect factor gene degQ from bacillus subtilis 168 and a strong promoter P43 are integrated in series onto a genome of the bacillus subtilis 168 by utilizing CRISPR gene editing to construct a strain B.subtilis ds; an acetyl coenzyme A synthetase gene acs of Escherichia coli BL21, an acetyl coenzyme A carboxylase gene accACD of Salmonella enterica and a biotin ligase gene bisA of Corynebacterium glutamicum are connected in series to an expression vector pHT43, and the expression vector pHT43 is introduced into a fengycin synthesis strain B.subtilis ds, so that a fengycin high-yield strain B.subtilis dspabacd is constructed; the constructed engineering strain is fermented and cultured in a shake flask to produce the fengycin.