146 results about "Salmonella escanaba" patented technology

The invention relates to a culture medium for simultaneous composite enrichment of five kinds of food-borne pathogenic bacteria, namely Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella, and a preparation method for the culture medium. Food-borne pathogenic bacteria are a significant reason to cause food positioning, so the rapid and accurate detection of the food-borne pathogenic bacteria has an important practical significance for preventing and controlling food safety incidents. The culture medium is characterized by comprising the following components: 10.0 g of peptone, 10.0 g of sodium chloride, 9.0 g of disodium hydrogen phosphate, 1.5 g of monopotassium phosphate, 0.1 g of cholate, 0.1 mg of potassium tellurite, 1.0 g of lithium chloride, 3.0 g of glucose, 2.0 g of mannitol, 2.5 g of sodium pyruvate, 1.0 g of aesculin and 1,000 mL of distilled water, wherein the pH value is 7.1 to 7.5. The culture medium can simultaneously enrich five kinds of target pathogenic bacteria, can be used for separation and identification of target bacteria, can also be used for the molecular detection of multiple pathogenic bacteria on the same detection platform, provides technical support for a method for rapidly detecting five kinds of pathogenic bacteria in food, and meets the requirement of simultaneous detection of five kinds of food-borne pathogenic bacteria.

The invention provides a lactobacillus plantarum AB-3 strain having an activity of resisting phytophthora melonis kalsura, of which the accession number is CGMCC No.9655. The lactobacillus plantarum AB-3 has effective inhibition properties on escherichia coli, salmonella typhimurium and shigella flexneri, has effective inhibition properties on the phytophthora melonis kalsura, fusarium oxysporum, penicillium roqueforti and glomerella cingulata and is excellent in thermostability. A fermentation supernate of the strain shows a significantly increased bacteria inhibition rate after treatment at 100 DEG C and is enhanced in bacteria inhibition activity through enzymolysis by a neutral protease. The fermentation supernate of the strain has a storage resistance property and is excellent in bacteria inhibition activity even after normal storage for 20 days.

The invention discloses a strain of lactobacillus salivarius ZLp4b and its application in preventing and treating diarrhea of young animals. The strain has broad-spectrum bacteriostasis and can inhibit the growth of various pathogenic bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus and PasteureUa multocida, etc. In addition, the strain has good in-vitro probiotic characteristics, including high gastrointestinal tolerance, high temperature tolerance and adhesion. As the lactobacillus salivarius of the invention is applied to feeding of mice and diarrhea piglets, lambs and calves, the secretion of immunoglobulin and cytokines in the serum of mice can be promoted, and the diarrhea symptoms of young animals can be cured or obviously alleviated.

The invention discloses a method for fast identifying clinical pathogens (such as staphylococcus aureus, salmonellae and escherichia coli). The method includes the following steps that near infrared spectrograms of staphylococcus aureus, salmonellae and escherichia coli samples are collected; PCA dimension reduction processing is carried out on the near infrared spectrograms of the staphylococcus aureus, salmonellae and escherichia coli samples; a Fisher discrimination function is set up for classification. The adopted principal component analysis method in combination with Fisher discriminance is introduced to identify the common pathogens, the types of the pathogens can be fast identified, and the method has the advantages of being high in accuracy and easy to operate, making a great contribution to follow-up clinical strain identification and having good application prospects.

The invention discloses a multiple rapid detection method for three food borne pathogenic bacteria, and detection primer sets and a detection kit thereof. An upstream primer and a downstream primer of a rapid detection primer set for Salmonella have sequences as represented by SEQ No.1 and SEQ No.2, respectively; an upstream primer and a downstream primer of a rapid detection primer set for Shigella have sequences as represented by SEQ No.3 and SEQ No.4, respectively; an upstream primer and a downstream primer of a rapid detection primer set for o Staphylococcus aureus have sequences as represented by SEQ No.5 and SEQ No.6, respectively. Bacterial genome DNA extracted from a sample to be tested is subjected to a LAMP reaction with the primer sets in a same reaction system, and the resultant of the reaction is identified to determine whether the sample contains Salmonella, Shigella or Staphylococcus aureus. Every primer set provided in the invention has strong specificity and can accurately detect genome DNAs of Salmonella, Shigella and Staphylococcus aureus in a same reaction system.

The invention discloses a salmonella phage RDP-NSA-19050 with a wide lysis spectrum, wherein the preservation number is CGMCC No.21415, the gene length is 87591 bp; after sequencing is carried out on whole genes, it is found that the salmonella phage RDP-NSA-19050 does not have virulence genes and lyogenic genes, and the salmonella phage RDP-NSA-19050 has a good lysis spectrum. The salmonella bacteriophage RDP-NSA-19050 provided by the invention can be used for cracking a plurality of pathogenic bacteria such as salmonella steulani, salmonella gallinarum, salmonella typhimurium, salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella enteritidis and salmonella kkinensis; the salmonella bacteriophage RDP-NSA-19050 has the characteristics of wide splitting spectrum and high splitting rate, can be independently used, can also be compounded with bacteriophages of other escherichia coli and salmonella and probiotics for use, and can effectively reduce the death rate of chicken infected with salmonella.

The invention relates to a detection assembly for detecting bacteria based on a bacteria fluorescent staining technique. A single antibody is utilized for detecting thalli which are stained by fluorescence; an escherichia coli O157:H7 antibody, a listeria monocytogenes antibody and a salmonella typhimurium antibody are sprayed on a test strip, and the test strip acts an immunochromatography test strip with three detection lines (T1, T2 and T3 lines). The detection assembly for detecting bacteria has the advantages that fluorochrome is used for pre-staining thalli, and the respective specific antibody is utilized again and is sprayed onto the T lines of the test strip so as to capture target thalli in a mixed sample, so that the thalli in the sample are detected rapidly and specifically; the detection assembly for detecting bacteria can be used for simultaneously detecting the food-borne pathogenic bacteria including the escherichia coli O157:H7 antibody, the listeria monocytogenes antibody and the salmonella typhimurium.

The invention belongs to the field of microbiological detection and discloses a method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in food simultaneously and rapidly. A special primer is designed aiming at salmonella typhimurium, salmonella paratyphi b and salmonella typhi, hydrazoic brominated c ingot processing is adopted to remove interference of dead bacteria, and finally deoxyribonucleic acids (DNAs) are extracted to perform multiplex polymerase chain reaction. Compared with a classical bacterium separating and identifying method and a serologic typing method, the method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in the food simultaneously and rapidly is rapid and accurate.

The invention provides a bacteriophage composition, a bacteriostatic agent and application thereof, and belongs to the technical field of bioengineering. The bacteriophage composition comprises a clostridium perfringens bacteriophage (CCTCC NO: M2020047), a salmonella bacteriophage (CCTCC NO: M2020710), an escherichia coli bacteriophage (CCTCC NO: M2020711) and a shigella flexneri bacteriophage (CCTCC NO: M209029). The bacteriophage composition has a strong lysis effect on clostridium perfringens, salmonella, escherichia coli and shigella flexneri, and can be used for inhibiting and/or eliminating clostridium perfringens, salmonella, escherichia coli and shigella flexneri; by inhibiting the overgrowth of clostridium perfringens, salmonella, escherichia coli and shigella flexneri, the occurrence of intestinal diseases of animals is effectively prevented and controlled; a safe bacteriophage antibacterial product source without toxic and side effects is provided for preventing and controlling animal intestinal diseases caused by clostridium perfringens, salmonella, escherichia coli and shigella flexneri.

The invention provides a pediococcus pentosaceus NHB-PpA9601. The pediococcus pentosaceus NHB-PpA9601 is separated from intestinal tracts of white feather broilers, and is preserved in China General Microbiological Culture Collection Center (CGMCC for short, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101), the preservation number is CGMCC NO.22646, and the preservation date is May 31, 2021. The pediococcus pentosaceus NHB-PpA9601 has the advantages that the obvious probiotic property is realized, the growth and reproduction of pathogenic bacteria such as intestinal pathogenic escherichia coli, staphylococcus aureus, salmonella suis, salmonella choleraesuis, salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmonella, shigella, bacillus perfringens, proteus penneri, aeromonas hydrophila and vibrio parahaemolyticus can be effectively inhibited; the pediococcus pentosaceus NHB-PpA9601 can be used for producing a wet-based fermented feed, and can be used for effectively improving intestinal health and increasing economic benefits.

The invention discloses a lipid type antibacterial composition. The lipid type antibacterial composition is extracted from black soldier fly larvae and contains 18.0 to 22.0 percent of glyceryl oleate, 7.7 to 10.0 percent of lauric acid and 15.5 to 18.0 percent of myristic acid. The lipid type antibacterial composition belongs to the field of biology; the composition has an excellent antibacterialeffect and at least can have a specific antibacterial effect on part of escherichia coli and enteritis type salmonella; meanwhile, the invention further discloses an extraction method, a detection method and application of the composition.

The invention discloses a detecting kit for five kinds of food-borne pathogenic bacteria and a detection method, and belongs to the field of molecular biology and food safety detection. The kit is mainly prepared from primer pairs of salmonella, primer pairs of staphylococcus aureus, primer pairs of escherichia coli O157:H7, primer pairs of vibrio parahaemolyticus, primer pairs of listeria monocytogenes, Taq DNA polymerase, 10*polymerase chain reaction buffer, deoxy-ribonucleoside triphosphate and MgCl2. In the detection process, a to-be-detected sample polymerase chain reaction template is prepared and added to the kit for polymerase chain reaction amplification, and then amplification products are subjected to electrophoresis and positive control. According to the detection method, the five kinds of pathogenic bacteria in food can be detected at the same time, and detection sensitivity is high. The detection process is simple, and popularization is easy.

The invention relates to bifidobacterium animalis which is resistant to acid and cholate, can survive at 15-25 DEG C, can regulate intestinal microbial flora and has an inhibiting effect on escherichia coli, salmonella typhimurium and staphylococcus aureus. The bifidobacterium animalis is preserved in CGMCC, has a preservation number of CGMCC No.19827, has the effects of increasing the number of lactic acid bacteria in intestinal tracts and reducing the number of escherichia coli, and can grow in cooperation with lactobacillus rhamnosus CGMCC NO.19826 and lactobacillus plantarum CGMCC NO.19825to jointly regulate the intestinal microbial flora.

The invention relates to a Chinese chive seed antibacterial tripeptide and a preparation method and use thereof, and belongs to the fields of foods and biomedicines. An antibacterial sequence is Ser-Ala-Asn (SAN); the polypeptide can inhibit growth of common bacteria including gram negative bacteria and gram positive bacteria. An in vivo experiment proves that the polypeptide can effectively inhibit growth of escherichia coli, staphylococcus aureus, salmonella and bacillus subtilis, and is small in hemolytic activity, so that the Chinese chive seed antibacterial tripeptide has important value in development and application of food preservatives and drugs.

The invention belongs to the technical field of biology, and particularly relates to application of a protein encoded by a salmonella phage gene as a lyase. The amino acid sequence of the protein is shown as SEQ ID NO.2, and the nucleotide sequence of the gene encoding the protein is shown as SEQ ID NO. 1. The invention finds for the first time that the protein shown as SEQ ID NO.2 has the effectof the lyase, by the protein, self-internal-cracking of gram-negative bacteria such as escherichia coli can be achieved, and the lyase has a high cracking effect and has a cracking effect on various different escherichia coli and salmonella.

The invention designs and builds up a new method for microscopic examination of escherichia coli and salmonella typhimurium based on germ growth. Through adding beta- lactam antibiotics in a to-be-tested sample solution, the germ is grown; film filtering and magnetic separation are combined so as to realize the specificity and high sensitivity detection of the escherichia coli and salmonella typhimurium, thus the germ number can be judged under an optical microscope; the method can be applied to a water sample; the whole detection process can be completed within 2 hours. The test verifies thatthe method can reduce the detection limit of escherichia coli and salmonella typhimurium to 5 cells and meet the requirement of fast detecting method. The method is simple in operation, low in cost and can be widely applied to the microscopic examination of the Gram negative bacteria in an actual sample.

The invention discloses PCR (polymerase chain reaction) detection primers for three bacteria and the application of the PCR detection primers. The three bacteria are alicyclobacillus aacillus, escherichia coli and salmonella. The upstream detection primer of the alicyclobacillus aacillus has the SEQIDNO.1, and the downstream detection primer has the SEQIDNO. 2. The upstream detection primer of the escherichia coli has the SEQIDNO.3, and the downstream detection primer has the SEQIDNO.4. The upstream detection primer of the salmonella has the SEQIDNO.5, and the downstream detection primer has the SEQIDNO.6. The multiple PCR detection method provided by the invention has the biggest advantages that the one PCR tube can be used for detecting three bacteria, the PCR detection efficiency is improved, and the errors caused by misoperation during detection are reduced.

The invention discloses a salmonella phage LPST144 and antibacterial application of a lyase of the salmonella phage LPST144. The phage is called after Salmonella Typhimurium Bacteriophage LPST144. Thesalmonella phage LPST144 disclosed by the invention and the lyase LysT15 thereof can crack salmonella; through specifically cracking the salmonella by employing the phage and the lyase thereof, the salmonella can be prevented from evolving, and the generation of drug-resistant strains is reduced; both the phage strain disclosed by the invention and the lyase thereof have hydrophilic phases, and spraying solutions or eluents are very easily prepared through the traditional method so as to disinfect environments or utensils; and the phage disclosed by the invention and the lyase thereof are free of toxic or side effects, can serve as food additives and have potential applications in dealing with food contamination.