95 results about "Salmonella kiel" patented technology

Disclosed are vaccines and immunogenic compositions which use live attenuated pathogenic bacteria, such as Salmonella, to deliver ectopic antigens to the mucosal immune system of vertebrates. The attenuated pathogenic bacteria are engineered to secrete the antigen into the periplasmic space of the bacteria or into the environment surrounding the bacteria. The vertebrate mounts a Th2-mediated immune response toward the secreted antigen.

Aqueous dilutions of acidic ternary compositions having unusually synergistic antimicrobial activity against Staphylococcus aureus and Salmonella choleraesuis microorganisms are disclosed. The compositions encompass ternary component mixtures of an alpha-hydroxycarboxylic acid, a monohydric water soluble alkanol and an anionic tenside having a Ternary Ratio residing within a defined phase region wherein synergistic antimicrobial activity is observed; whereas mixtures outside of the defined phase region and those lacking any one of the components do not exhibit synergistic activity. Compositions, methods and articles employing the inventive ternary synergistic compositions, and their utility in sanitizing and disinfecting hard surfaces are described.

The invention adopts double-function gold magnetic nanoparticles and provides a detection method which integrates an immunomagnetic bead capturing technology and an immunochromatography technology and is used for rapidly detecting salmonellas. Immunomagnetic separation and immunochromatography are organically integrated, the step of eluting the salmonellas from immunomagnetic beads is eliminated and the capture efficiency is improved; the step of spraying colloidal gold on a bonding mat is eliminated, the immunological reaction is more uniform and a variable coefficient is small in the quantitative detection; workload and probability of mixed bacterium pollution are reduced. The detection method adopts the basic thinking of exploring the mode of effectively combining the gold magnetic nanoparticles and an antibody, optimizing the conditions of enriching the salmonellas and carrying out rapid quantitative detection by using the immunochromatography technology as a vector and using the double antibodies sandwich as a detection principle.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU/mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU/mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

The invention relates to a complex enrichment medium used for salmonella, vibrio parahaemolyticus and comma bacillus and a method for preparing the same. The formulation components of the complex enrichment medium in weight portion are as follows: 0.5 to 1.5 portions of buffer peptone water, 1000 portions of distilled water, 1.0 to 5.0 portions of glucose, 1.0 to 5.0 portions of mannitol, 0.03 to 0.06 portion sodium pyruvate, 0.5 to 2.5 portions of anhydrous sodium sulfite, 1.0 to 10.0 portions of sodium citrate, 5.0 to 25.0 portions of sodium chloride, 1 to 5 portions of cholate, and 0.0005 to 0.0025 portion of potassium tellurite. The method comprises the following steps: firstly, adding the buffer peptone water and the like into the distilled water, and sterilizing the mixture after heating and melting; and secondly, cooling the mixture to 50 DEG C, and then adding the potassium tellurite into the mixture to be mixed evenly. The complex enrichment medium can integrate two steps of primary enrichment and selective enrichment of bacterial detection to shorten the enrichment time to 24 hours, can perform enrichment on three target pathogens at the same time, and can inhibit the growth of other microorganisms.

The invention provides lactobacillus plantarum resistant to enteritis salmonella infection and the application thereof and belongs to the technical field of microorganisms.The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Sep, 24th, 2015 with the preservation number of CGMCC No.11446.The lactobacillus plantarum CGMCC No.11446 has high acid and cholate resistance, and has a remarkable inhibition effect on growth of enteritis salmonella.Adhesion of enteritis salmonella to enterocyte HT-29 is strongly inhibited, and enteritis salmonella infection is prevented or relieved.The lactobacillus plantarum CGMCC No.11446 can be used for preparing pharmaceutical compositions for preventing or relieving enteritis salmonella, food containing activity lactobacillus and fermented food, and has broad application prospects.

The invention relates to the biological detection kit and method, in particular to a salmonella detection kit based on the fimY gene. The detection kit contains the following specific primers: a forward outer primer F3 shown in the SEQ ID NO.1, a reverse outer primer B3 shown in the SEQ ID NO.2, a forward inner primer FIP shown in the SEQ ID NO.3, a reverse inner primer BIP shown in the SEQ ID NO.4, a forward ring primer LF shown in the SEQ ID NO.5 and a reverse ring primer LB shown in the SEQ ID NO.6. The kit is prepared on the basis of the loop-mediated isothermal amplification technology, has high specificity and sensitivity and can detect salmonella easily and fast without using any expensive instrument, thus the kit is particularly suitable for the basic-level medical unit.

The present invention relates to live attenuated Salmonella strains comprising a first attenuating mutation, that are not capable of making functional RecA. The invention also relates to these bacteria for use in vaccines. Furthermore, the invention relates to vaccines based upon these bacteria, to the use of such bacteria in the manufacture of vaccines and to methods for the preparation of such vaccines.

The invention provides a biosensor for detecting salmonella, which includes: an aptamer (Apt), a template (T), a hairpin probe I (H1), a hairpin probe II (H2), heme, potassium ion (K+), cysteine, nanogold modified by a Raman dye, and a buffer solution. The biosensor can be used for detecting the salmonella in foods through surface Raman enhancement. The biosensor has high specificity and detection sensitivity and has gentle reaction conditions and high reaction speed. Compared with other optical detection methods, the biosensor is easy to operate and has short detection period. The detectionprocess is achieved in homo-phase, so that complexness of the method is reduced. The biosensor is free of enzymes, so that process cost of the biosensor is reduced. The biosensor can fit the demand oflow cost in industrialization.

The invention discloses an apparatus and method for synthesizing a superparamagnetic microcapsule based on microfluidic technology; a polydimethylsiloxane (PDMS) microchip is used to synthesize a 'shell-core' microcapsule-like delivery carrier (in figure 1), and shell (9) is made of naturally degradable hydrogel composed of superparamagnetic nanoparticles; a microcapsule core is of liquid form covering salmonella (12) and degradation liquid (11); salmonella density and release time are controlled by studying synthetic control factors. The invention is intended to use microfluidic technology to synthesize 'shell-core' superparamagnetic microcapsule-like delivery carrier covering salmonella, the survival rate of magnetotactic salmonella during delivery is guaranteed; through quick response to an external magnetic field, three-level precision delivery and tracing detection is achieved, the therapeutic effect and targeting efficiency of salmonella for tumors are improved, the fusion of oncology and robotics is promoted, and new theory and method are provided for the therapy of advanced colorectal cancer.

This invention relates to a novel method for growing attenuated mutant Salmonella typhi strains lacking galactose epimerase activity and harboring a recombinant DNA molecule. The method comprises the step of culturing said Salmonella typhi strain without adding glucose to the medium during the fermentation with a starting glucose amount that is depleted before reaching the stationary phase. The invention further relates to attenuated mutant Salmonella typhi strains obtainable by said method and to an attenuated mutant Salmonella typhi strain harboring a recombinant DNA molecule encoding a VEGF receptor protein for use as a vaccine.

The invention discloses a method and a kit for rapidly detecting salmonella living cells in feed by combining ethidium monoazide (EMA) and polymerase chain reaction (PCR). The kit comprises salmonella positive control, salmonella negative control, a Taq premix enzyme and a primer mixture, DL Marker2000, an EMA solution and a covalent cross-linking exposure device. The method is low in cost and convenient and fast in operation, and can objectively reflect the existence condition of the salmonella living cells in the feed, thus providing an effective means for rapid and accurate detection of the salmonella in the feed.

The invention discloses a composite enrichment culture medium capable of simultaneously enriching three food-borne pathogenic bacteria, namely Listeria monocytogenes, Escherichia coli O157:H7 and salmonella, and a preparation method. The formula of the culture medium comprises the following components of, in parts by weight, 15.0-19.0 parts of tryptone, 2.7-3.3 parts of peptone, 5.5-6.5 parts of yeast extract, 4.5-5.5 parts of sodium chloride, 2.25-2.75 parts of dipotassium phosphate, 8.6-10.6 parts of disodium hydrogen phosphate, 1.22-1.48 parts of potassium dihydrogen phosphate, 24.0-30.0 parts of peptone, 14.0-18.0 parts of beef powder, 10.0-14.0 parts of soy peptone, 2.0-5.0 parts of glucose, 0.10-0.30 part of actinomycete ketone, 2.5-3.5 parts of mannitol, 4.5-6.5 parts of sodium pyruvate, 0.00125-0.00300 part of acridine yellow, 0.0007813-0.0032500 part of fosfomycin sodium and 1000 parts of distilled water, and the pH value of theculture medium is 6.8-7.4. The single enrichmenteffect of the composite enrichment culture medium is superior to that of respective selective enrichment liquid, growth of non-target bacteria can be well inhibited, under the condition that non-target bacteria exist, the culture medium can further achieve constant-speed and rapid proliferation of the three target bacteria.

There is provided a vaccine composition comprising a combination of a genetic deletion mutant S. typhimurium microorganism and a genetic deletion mutant E. coli microorganism, suitable for mass application to poultry. Also provided is a safe and effective method to protect poultry against the ravages of E. coli and Salmonella infection and disease.

The invention provides a group of molecular targets for detecting salmonella. The molecular targets comprise nucleotide sequences as shown in SEQ ID NO. 1-13. The invention also provides a group of primers for detecting the salmonella molecular targets in the claim 1, and the nucleotide sequences of the primers are shown as SEQ ID NO.14-39. The primers are adopted to perform PCR amplification on the specific targets, whether a sample contains salmonella or not is judged by detecting whether an amplification product contains a target strip or not, the detection cost is reduced, and the method is more practical; the specific molecular targets, namely 13 conservative nucleic acids, have single specificity, the detection result is specific, and the result judgment is simple.

A recombineered Salmonella typhi Ty21a, compositions and vaccines comprising such a Ty21a, and a method for recombineering comprising inserting a large antigenic region into a bacterial chromosome for the purpose of making multivalent vaccines to protect against one or more disease agents are described herein.

The invention discloses a salmonella efficient traceless gene editing system and an application thereof. Lambda Red recombinase is used for promoting double exchange of template DNA and genome targetsites, through combination of a CRISPR/Cas9 system as a screening means, and through combination of a one-step method, homologous recombination is used for quickly constructing shooting plasmid, and insertion, replacement or knockout of genes can be realized. A homologous formwork is used for replacing the genome DNA, and other fragments are not remained. Genome editing can be completed within 3-4days, the experiment strength can be reduced, and the experiment cycle can be shortened.

The invention belongs to the technical field of biology, and discloses a leading stabilizing element for enhancing activity and expression of exogenous protein in bacteria. The leading stabilizing element is 208 amino acid residue segments at the N end of the salmonella enteric subspecies chlamydia typhimurium strain RM13672SspH1 protein, the nucleotide sequence is shown in SEQ.ID. NO: 1, and the amino acid sequence is shown in SEQ.ID. NO: 2. the leading stabilizing element for enhancing activity and expression of the exogenous protein in the bacteria can be used as a tool for improving the protein yield and the protein activity in the industry or scientific research, and is beneficial to scientific research, biological medicine industrial production and preparation of anti-infectious disease vaccines. Besides, glutathione S transferase (GST) is a protein containing 211 amino acids, is the most common stable polypeptide expressed by the protein, but has great effect difference on different target proteins. The identified SsppH1 leading stabilizing element can provide a new choice so as to obtain ideal expression of active protein.

A method for inhibiting or preventing Salmonella invasion of the intestinal epithelium in a subject, the method comprising enterally administering to the subject a pharmaceutically effective amount of a fatty acid dissolved or suspended in a pharmaceutically acceptable carrier, wherein the fatty acid contains 10 to 30 carbon atoms, wherein the fatty acid is more particularly an unsaturated fatty acid containing 1-4 carbon-carbon double bonds and/or 1 or 2 carbon-carbon triple bonds, or the fatty acid is more particularly a cis-unsaturated fatty acid, or the fatty acid is more particularly a cis-2-unsaturated fatty acid having the formula:

wherein n is an integer of 6-26, and the fatty acid optionally includes a second carbon-carbon double bond resulting from removal of two hydrogen atoms on adjacent carbon atoms.

An inducible expression system is provided that includes an inducible ansB promoter co-dependently regulatable by cyclic AMP and anaerobiosis. The expression system is particularly suited to chromosomal expression of immunogenic proteins in attenuated bacterial vaccines. Protein expression from an E. coli-derived ansB promoter is particularly effective in a Salmonella host bacterium.

The invention discloses a quadruple PCR detection primer group for simultaneously detecting shigella, salmonella, clostridium welchii and escherichia coli, which comprises four pairs of primers, namely a shigella upstream primer pair and a shigella downstream primer pair which are respectively SEQIDNO: 1 and SEQIDNO: 2; an upstream primer pair and a downstream primer pair of salmonella are respectively SEQ ID NO: 3 and SEQ ID NO: 4; the clostridium welchii A type primer pair is shown as SEQ ID NO: 5 and SEQ ID NO: 6 respectively; and the primer pairs of the escherichia coli are respectively SEQ ID NO: 7 and SEQ ID NO: 8. A multiple detection method is established for common enteropathogenic bacteria in chicken farms, on the basis of high detection accuracy and good specificity, the detection time is shortened, the detection efficiency is improved, and the multiple detection method is more beneficial to application in large-scale breeding production.

The invention discloses a Salmonella/Shigella/Staphylococcus aureus composite enriched medium and a preparation method thereof. The medium comprises the following components in parts by mass: 5-15 parts of tryptone, 2.5-7.5 parts of yeast extract, 5-15 parts of sodium chloride, 1-3 parts of glucose, 1.5-3 parts of disodium hydrogen phosphate, 1-3 parts of mannitol, 1-3 parts of sodium pyruvate, 1-3 parts of glycine and 1000 parts of distilled water. The pH value is 7.2-7.5. The preparation method comprises the following steps: adding all the components to the distilled water, dissolving by stirring and autoclaving. The Salmonella, Shigella and Staphylococcus aureus are main pathogens in need of detection in national standard of food sanitation. The composite enriched medium can be utilized to implement constant-speed and high-speed proliferation of the Salmonella, Shigella and Staphylococcus aureus. After proliferation for 16 hours, the composite enriched medium can be directly used for high-flux detection of multiplex PCR (polymerase chain reaction), gene chips, microfluidic chips and the like to screen the three pathogens.

The invention provides a pigeon source lactobacillus johnsonii strain, and the preservation number of the pigeon source lactobacillus johnsonii strain is CGMCC (China General Microbiological Culture Collection Center) No. 21215. The bacterial strain disclosed by the invention has excellent antibacterial performance, especially has a remarkable antibacterial effect on pigeon-derived salmonella typhimurium, and the bacterial strain and related microbial agents provided by the invention can be used for reducing inflammatory response of young pigeons, reducing liver displacement of salmonella and inhibiting reduction of weight of infected pigeons, and have good development and application prospects.

The invention discloses an application of attenuated salmonella typhimurium and genetically engineered bacteria thereof in preparation of medicine for treating acute leukemia, and relates to the technical field of medicines. The genetically engineered bacteria carry recombinant expression plasmids with cloned L-methioninase genes, can continuously express L-methioninase in tumor tissues, consumesa large amount of methionine and other nutrient substances, enables tumor cells to lack nutrition and grow slowly, and has a remarkable growth inhibition effect on acute leukemia cells, so that the genetically engineered bacteria can be used for preparing the medicine for treating the acute leukemia.