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103 results about "Salmonella kiel" patented technology
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Immunogenic compositions and vaccines comprising carrier bacteria that secrete antigens
InactiveUS20040101531A1Snake antigen ingredientsDepsipeptidesMucosal Immune ResponsesAntigen delivery
Disclosed are vaccines and immunogenic compositions which use live attenuated pathogenic bacteria, such as Salmonella, to deliver ectopic antigens to the mucosal immune system of vertebrates. The attenuated pathogenic bacteria are engineered to secrete the antigen into the periplasmic space of the bacteria or into the environment surrounding the bacteria. The vertebrate mounts a Th2-mediated immune response toward the secreted antigen.
Owner:WASHINGTON UNIV IN SAINT LOUIS
Peptide-based vaccine for influenza
A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.
Owner:YEDA RES & DEV CO LTD
Synergistic acidic ternary biocidal compositions
InactiveUS20060293214A1Great anti-microbial activitySurprising synergistic antimicrobial efficacyBiocideAnionic surface-active compoundsBiotechnologySalmonella kiel
Aqueous dilutions of acidic ternary compositions having unusually synergistic antimicrobial activity against Staphylococcus aureus and Salmonella choleraesuis microorganisms are disclosed. The compositions encompass ternary component mixtures of an alpha-hydroxycarboxylic acid, a monohydric water soluble alkanol and an anionic tenside having a Ternary Ratio residing within a defined phase region wherein synergistic antimicrobial activity is observed; whereas mixtures outside of the defined phase region and those lacking any one of the components do not exhibit synergistic activity. Compositions, methods and articles employing the inventive ternary synergistic compositions, and their utility in sanitizing and disinfecting hard surfaces are described.
Owner:THE CLOROX CO
Salmonella enrichment and rapid detection method
ActiveCN103439497AActivity is not affectedHigh coupling rateMaterial analysisSalmonella kielMagnetic bead
The invention adopts double-function gold magnetic nanoparticles and provides a detection method which integrates an immunomagnetic bead capturing technology and an immunochromatography technology and is used for rapidly detecting salmonellas. Immunomagnetic separation and immunochromatography are organically integrated, the step of eluting the salmonellas from immunomagnetic beads is eliminated and the capture efficiency is improved; the step of spraying colloidal gold on a bonding mat is eliminated, the immunological reaction is more uniform and a variable coefficient is small in the quantitative detection; workload and probability of mixed bacterium pollution are reduced. The detection method adopts the basic thinking of exploring the mode of effectively combining the gold magnetic nanoparticles and an antibody, optimizing the conditions of enriching the salmonellas and carrying out rapid quantitative detection by using the immunochromatography technology as a vector and using the double antibodies sandwich as a detection principle.
Owner:NANCHANG UNIV
Method for sensitively, simply and conveniently detecting bacteria
InactiveCN102841198ASimple stepsEasy to detectFluorescence/phosphorescenceSalmonella kielFluorescence microscope
The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU / mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU / mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.
Owner:WUHAN UNIV
Culture medium for composite enrichment of salmonella, Vibrio parahaemolyticus and Vibrio cholerae, and preparation thereof
InactiveCN101412977AShorten enrichment timeBacteriaMicrobiological testing/measurementBacteroidesSulfite salt
The invention relates to a complex enrichment medium used for salmonella, vibrio parahaemolyticus and comma bacillus and a method for preparing the same. The formulation components of the complex enrichment medium in weight portion are as follows: 0.5 to 1.5 portions of buffer peptone water, 1000 portions of distilled water, 1.0 to 5.0 portions of glucose, 1.0 to 5.0 portions of mannitol, 0.03 to 0.06 portion sodium pyruvate, 0.5 to 2.5 portions of anhydrous sodium sulfite, 1.0 to 10.0 portions of sodium citrate, 5.0 to 25.0 portions of sodium chloride, 1 to 5 portions of cholate, and 0.0005 to 0.0025 portion of potassium tellurite. The method comprises the following steps: firstly, adding the buffer peptone water and the like into the distilled water, and sterilizing the mixture after heating and melting; and secondly, cooling the mixture to 50 DEG C, and then adding the potassium tellurite into the mixture to be mixed evenly. The complex enrichment medium can integrate two steps of primary enrichment and selective enrichment of bacterial detection to shorten the enrichment time to 24 hours, can perform enrichment on three target pathogens at the same time, and can inhibit the growth of other microorganisms.
Owner:SOUTH CHINA UNIV OF TECH
Lactobacillus plantarum resistant to enteritis salmonella infection and application thereof
ActiveCN105779350AGrowth inhibitionReduce adhesionAntibacterial agentsMilk preparationSalmonella urbanaMicrobiological culture
The invention provides lactobacillus plantarum resistant to enteritis salmonella infection and the application thereof and belongs to the technical field of microorganisms.The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Sep, 24th, 2015 with the preservation number of CGMCC No.11446.The lactobacillus plantarum CGMCC No.11446 has high acid and cholate resistance, and has a remarkable inhibition effect on growth of enteritis salmonella.Adhesion of enteritis salmonella to enterocyte HT-29 is strongly inhibited, and enteritis salmonella infection is prevented or relieved.The lactobacillus plantarum CGMCC No.11446 can be used for preparing pharmaceutical compositions for preventing or relieving enteritis salmonella, food containing activity lactobacillus and fermented food, and has broad application prospects.
Owner:BEIJING SCITOP BIO TECH CO LTD
Method for detecting salmonella based on nucleic acid chromatography biosensing technology
The invention relates to a method for detecting salmonella based on a nucleic acid chromatography biosensing technology. According to a virulence gene invA of salmonella, loop-mediated isothermal amplification primers (SEQ ID NO:1-4) are designed, and through the combination with a nano-enzyme nucleic acid test strip, the salmonella detection method based on an LAMP nano-enzyme sensor is established. The method for detecting salmonella based on the nucleic acid chromatography biosensing technology can be successfully used for distinguishing live bacterial cells from dead bacterial cells, and the lower limit of detection on salmonella can reach 10 CFU / mL.
Owner:CHINA AGRI UNIV
Method for producing organic manure through harmless treatment of fowl, livestock feces
InactiveCN1800109AImprove crop qualityIncrease crop yieldClimate change adaptationExcrement fertilisersSalmonella danWater content
The invention discloses a method for using transit fecal harmless treatment to make organic endorse fertilizer in the field of fecal harmless treatment and fertilizer making method. It uses methyphrrolidine and the high temperature of the fermenting course to kill the colicin, Salmonella and parasite ovum to achieve the fecal harmless treatment. It uses the fecal and the urine as basic material and the abandon cigar to adjust the PH value, the abandon tea clinker to adjust the porosity degree and the abandon material to adjust the water content. The fertilizer component is that the organic material >=35, the azophoska total content >=4, PH value 7.5-9.0, the water 26-35, the product PH value 7.5-9.0.
Owner:何炎森
Salmonella detection kit based on fimY gene and detection method thereof
InactiveCN102140519AGuaranteed reliabilityStrong specificityMicrobiological testing/measurementAgainst vector-borne diseasesMedical unitSalmonella kiel
The invention relates to the biological detection kit and method, in particular to a salmonella detection kit based on the fimY gene. The detection kit contains the following specific primers: a forward outer primer F3 shown in the SEQ ID NO.1, a reverse outer primer B3 shown in the SEQ ID NO.2, a forward inner primer FIP shown in the SEQ ID NO.3, a reverse inner primer BIP shown in the SEQ ID NO.4, a forward ring primer LF shown in the SEQ ID NO.5 and a reverse ring primer LB shown in the SEQ ID NO.6. The kit is prepared on the basis of the loop-mediated isothermal amplification technology, has high specificity and sensitivity and can detect salmonella easily and fast without using any expensive instrument, thus the kit is particularly suitable for the basic-level medical unit.
Owner:SICHUAN AGRI UNIV
Salmonella vaccine
The present invention relates to live attenuated Salmonella strains comprising a first attenuating mutation, that are not capable of making functional RecA. The invention also relates to these bacteria for use in vaccines. Furthermore, the invention relates to vaccines based upon these bacteria, to the use of such bacteria in the manufacture of vaccines and to methods for the preparation of such vaccines.
Owner:INTERVET INT BV
Biosensor for detecting salmonella
ActiveCN108103146AAchieve high specificity detectionAchieve oxidationMicrobiological testing/measurementRaman scatteringHemePotassium ions
The invention provides a biosensor for detecting salmonella, which includes: an aptamer (Apt), a template (T), a hairpin probe I (H1), a hairpin probe II (H2), heme, potassium ion (K+), cysteine, nanogold modified by a Raman dye, and a buffer solution. The biosensor can be used for detecting the salmonella in foods through surface Raman enhancement. The biosensor has high specificity and detection sensitivity and has gentle reaction conditions and high reaction speed. Compared with other optical detection methods, the biosensor is easy to operate and has short detection period. The detectionprocess is achieved in homo-phase, so that complexness of the method is reduced. The biosensor is free of enzymes, so that process cost of the biosensor is reduced. The biosensor can fit the demand oflow cost in industrialization.
Owner:UNIV OF JINAN
Apparatus and method for synthesizing superparamagnetic microcapsule based on microfluidic technology
ActiveCN106693158AAvoid damageGuaranteed survival rateMedical devicesLaboratory glasswaresThree levelRelease time
The invention discloses an apparatus and method for synthesizing a superparamagnetic microcapsule based on microfluidic technology; a polydimethylsiloxane (PDMS) microchip is used to synthesize a 'shell-core' microcapsule-like delivery carrier (in figure 1), and shell (9) is made of naturally degradable hydrogel composed of superparamagnetic nanoparticles; a microcapsule core is of liquid form covering salmonella (12) and degradation liquid (11); salmonella density and release time are controlled by studying synthetic control factors. The invention is intended to use microfluidic technology to synthesize 'shell-core' superparamagnetic microcapsule-like delivery carrier covering salmonella, the survival rate of magnetotactic salmonella during delivery is guaranteed; through quick response to an external magnetic field, three-level precision delivery and tracing detection is achieved, the therapeutic effect and targeting efficiency of salmonella for tumors are improved, the fusion of oncology and robotics is promoted, and new theory and method are provided for the therapy of advanced colorectal cancer.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes
InactiveCN109971683AEasy to prepareIsokinetic proliferationBacteriaMicroorganism based processesEscherichia coliDipotassium phosphate
The invention discloses a composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes and a preparation method thereof. The composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes comprises 16.0-18.0 parts of tryptone, 2.0-4.0 parts of peptone, 5.0-7.0 parts of yeast extract, 10.0-20.0 parts of sodium chloride, 2.4-2.6 parts of dipotassium phosphate, 9.5-9.7 parts of disodium hydrogen phosphate, 1.34-1.36 parts of monopotassium phosphate, 0.005-0.020 part of acriflavine, 2.0-8.0 pars of magnesium chloride, 1.0-4.0 parts of glucose, 1.0-4.0 parts of sodium pyruvate and 1000 parts of distilled water, and has pH of 6.8-7.4. The composite enrichment medium herein allows three target pathogens to be enriched at a high, constant speed;after 16-24 hours of enrichment, quick detection of the three target pathogens can be implemented to provide a diagnostic report through high-flux detection devices, such as multiple PCR (polymerase chain reaction), multi-channel SPR (surface plasma resonance) biosensors, and multi-channel BLI (biolayer interferometry) biosensors.
Owner:JILIN UNIV
Method for Producing High Yield Attenuated Salmonella Strains
ActiveUS20140349274A1Increased cell yieldSafe and economic manufactureBacteriaMicrobiological testing/measurementSalmonella kielVEGF receptors
This invention relates to a novel method for growing attenuated mutant Salmonella typhi strains lacking galactose epimerase activity and harboring a recombinant DNA molecule. The method comprises the step of culturing said Salmonella typhi strain without adding glucose to the medium during the fermentation with a starting glucose amount that is depleted before reaching the stationary phase. The invention further relates to attenuated mutant Salmonella typhi strains obtainable by said method and to an attenuated mutant Salmonella typhi strain harboring a recombinant DNA molecule encoding a VEGF receptor protein for use as a vaccine.
Owner:VAXIMM
Rearranged bacterial virus bacteriolysis gene, perforating vector thereof and use in vaccine preparation
InactiveCN101503694AImprove punching effectHole punching worksAntibacterial agentsMicroorganism based processesCrabtreellaSalmonella
The invention discloses a rearranged phage bacteriolytic gene, as well as a perforation vector and application thereof in vaccine preparation. The rearranged phage bacteriolytic gene has a nucleotide sequence shown in SEQ ID NO:1. An efficient perforation vector can be obtained by operationally connecting the rearranged phage bacteriolytic gene with a prokaryotic expression vector. The perforation vector is transformed into avian salmonella and then added with value; the expression of the rearranged phage bacteriolytic gene is induced; and formed bacterial ghosts are collected so as to obtain avian salmonella bacterial-ghost vaccine. The bacterial-ghost vaccine prepared in the invention can be applied without concentration. The avian salmonella bacterial-ghost vaccine is good in safety. After chicks are inoculated with the avian salmonella bacterial-ghost vaccine, the content of antibodies in every immune chick can be remarkably increased, so that every immune chick can be effectively protected against attacking toxins of avian salmonella, which shows that the avian salmonella bacterial-ghost vaccine has good immune protection effect.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Method and kit for rapidly detecting salmonella living cells in feed by combining ethidium monoazide (EMA) and polymerase chain reaction (PCR)
InactiveCN102643926ADoes not affect amplificationAmplification blockingMicrobiological testing/measurementAgainst vector-borne diseasesBiotechnologySalmonella kiel
The invention discloses a method and a kit for rapidly detecting salmonella living cells in feed by combining ethidium monoazide (EMA) and polymerase chain reaction (PCR). The kit comprises salmonella positive control, salmonella negative control, a Taq premix enzyme and a primer mixture, DL Marker2000, an EMA solution and a covalent cross-linking exposure device. The method is low in cost and convenient and fast in operation, and can objectively reflect the existence condition of the salmonella living cells in the feed, thus providing an effective means for rapid and accurate detection of the salmonella in the feed.
Owner:河南省兽药监察所
Beta-1,4-mannobiose-containing composition
InactiveCN1717494AInhibition of attachmentAntibacterial agentsOrganic active ingredientsBiotechnologySalmonella kiel
Owner:JAPAN NUTRITION
Composite culture medium capable of simultaneously enriching three food-borne pathogenic bacteria and preparation method
InactiveCN112159787AGrowth inhibitionEasy to manufactureBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention discloses a composite enrichment culture medium capable of simultaneously enriching three food-borne pathogenic bacteria, namely Listeria monocytogenes, Escherichia coli O157:H7 and salmonella, and a preparation method. The formula of the culture medium comprises the following components of, in parts by weight, 15.0-19.0 parts of tryptone, 2.7-3.3 parts of peptone, 5.5-6.5 parts of yeast extract, 4.5-5.5 parts of sodium chloride, 2.25-2.75 parts of dipotassium phosphate, 8.6-10.6 parts of disodium hydrogen phosphate, 1.22-1.48 parts of potassium dihydrogen phosphate, 24.0-30.0 parts of peptone, 14.0-18.0 parts of beef powder, 10.0-14.0 parts of soy peptone, 2.0-5.0 parts of glucose, 0.10-0.30 part of actinomycete ketone, 2.5-3.5 parts of mannitol, 4.5-6.5 parts of sodium pyruvate, 0.00125-0.00300 part of acridine yellow, 0.0007813-0.0032500 part of fosfomycin sodium and 1000 parts of distilled water, and the pH value of theculture medium is 6.8-7.4. The single enrichmenteffect of the composite enrichment culture medium is superior to that of respective selective enrichment liquid, growth of non-target bacteria can be well inhibited, under the condition that non-target bacteria exist, the culture medium can further achieve constant-speed and rapid proliferation of the three target bacteria.
Owner:JILIN UNIV
Avian vaccine composition for the protection of poultry against disease and infection caused by E. coli and Salmonella
ActiveUS7297338B2Safe and effectiveAntibacterial agentsBacterial antigen ingredientsDiseaseMicroorganism
There is provided a vaccine composition comprising a combination of a genetic deletion mutant S. typhimurium microorganism and a genetic deletion mutant E. coli microorganism, suitable for mass application to poultry. Also provided is a safe and effective method to protect poultry against the ravages of E. coli and Salmonella infection and disease.
Owner:ZOETIS SERVICE LLC
Novel specific molecular targets for identifying salmonella and rapid detection method thereof
ActiveCN111057775AShort detection timeThe test result is specificMicrobiological testing/measurementMicroorganism based processesNucleotideSalmonella kiel
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Multifunctional oral vaccine based on chromosome recombineering
A recombineered Salmonella typhi Ty21a, compositions and vaccines comprising such a Ty21a, and a method for recombineering comprising inserting a large antigenic region into a bacterial chromosome for the purpose of making multivalent vaccines to protect against one or more disease agents are described herein.
Owner:UNITED STATES OF AMERICA
Inspection method and verification for salmonella in lip care cosmetics
InactiveCN102533931ATo achieve detection needsFacilitate rapid quantificationMicrobiological testing/measurementMicroorganism based processesConfirmation testFood safety
The invention relates to an inspection method and verification for salmonella in lip care cosmetics. The inspection method comprises the following steps of: 1, performing resuscitation and hyperplasia culture on a sample; 2, separating and culturing by using a chromogenic medium; 3, performing an initial identification test on a culture; 4, performing microscopic examination; 5, performing a biochemical test; and 6, performing a serum agglutination test, wherein 5 and 6 are typical salmonella confirmation tests. By combining a method for detecting the salmonella in the national food safety standard, the requirement for detecting the salmonella in the lip care cosmetics is met, and due to an obvious selectivity effect, the interference of other bacteria in the sample is avoided, and the salmonella is quickly detected. The method provides theoretical guidance for inspecting the salmonella in the lip care cosmetics, and has important instructive significance for improving the quality of the lip care cosmetics.
Owner:SHAANXI INST FOR FOOD & DRUG CONTROL
Salmonella efficient traceless gene editing system and application thereof
PendingCN111454927AQuick removalImprove operational efficiencyHydrolasesStable introduction of DNAGenome editingSalmonella kiel
The invention discloses a salmonella efficient traceless gene editing system and an application thereof. Lambda Red recombinase is used for promoting double exchange of template DNA and genome targetsites, through combination of a CRISPR / Cas9 system as a screening means, and through combination of a one-step method, homologous recombination is used for quickly constructing shooting plasmid, and insertion, replacement or knockout of genes can be realized. A homologous formwork is used for replacing the genome DNA, and other fragments are not remained. Genome editing can be completed within 3-4days, the experiment strength can be reduced, and the experiment cycle can be shortened.
Owner:CHANGZHOU HIGH TECH RES INST OF NANJING UNIV +1
Leading stabilizing element for enhancing activity and expression of exogenous protein in bacteria
ActiveCN113461790AHigh expressionHigh activityMicrobiological testing/measurementDepsipeptidesTyphimurium strainInfectious Disorder
The invention belongs to the technical field of biology, and discloses a leading stabilizing element for enhancing activity and expression of exogenous protein in bacteria. The leading stabilizing element is 208 amino acid residue segments at the N end of the salmonella enteric subspecies chlamydia typhimurium strain RM13672SspH1 protein, the nucleotide sequence is shown in SEQ.ID. NO: 1, and the amino acid sequence is shown in SEQ.ID. NO: 2. the leading stabilizing element for enhancing activity and expression of the exogenous protein in the bacteria can be used as a tool for improving the protein yield and the protein activity in the industry or scientific research, and is beneficial to scientific research, biological medicine industrial production and preparation of anti-infectious disease vaccines. Besides, glutathione S transferase (GST) is a protein containing 211 amino acids, is the most common stable polypeptide expressed by the protein, but has great effect difference on different target proteins. The identified SsppH1 leading stabilizing element can provide a new choice so as to obtain ideal expression of active protein.
Owner:SHANXI UNIV
Long chain fatty acid formulations and their use in inhibiting salmonella infection
PendingUS20200330420A1Promote growthHinders its propagationOrganic active ingredientsAccessory food factorsLong chain fatty acidSalmonella kiel
A method for inhibiting or preventing Salmonella invasion of the intestinal epithelium in a subject, the method comprising enterally administering to the subject a pharmaceutically effective amount of a fatty acid dissolved or suspended in a pharmaceutically acceptable carrier, wherein the fatty acid contains 10 to 30 carbon atoms, wherein the fatty acid is more particularly an unsaturated fatty acid containing 1-4 carbon-carbon double bonds and / or 1 or 2 carbon-carbon triple bonds, or the fatty acid is more particularly a cis-unsaturated fatty acid, or the fatty acid is more particularly a cis-2-unsaturated fatty acid having the formula:wherein n is an integer of 6-26, and the fatty acid optionally includes a second carbon-carbon double bond resulting from removal of two hydrogen atoms on adjacent carbon atoms.
Owner:CORNELL UNIVERSITY
Primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, kit and detection method thereof
ActiveCN105331677ADetection time is shortEasy to operateMicrobiological testing/measurementMicroorganism based processesMicroorganismMaterial resources
The invention is used for the field of food microorganism safe detection, and provides a primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, a kit and a detection method thereof. The primer group used for the multiple PCR detection, the kit and the detection method can combine and use species-specificity discrimination gene and drug resistance-related gene, and the technical effects of wide detection cover scope, strong specificity and high sensitivity can be realized, detection time consumption is short, operation is simple, equipment requirement is low, flux is high; manpower, material resource and financial resource can be saved, the primer group used for the multiple PCR detection, the kit and the detection method are suitable for rapid detection requirement, and are easily popularized and applied in practical production.
Owner:COFCO NUTRITION & HEALTH RES INST +1
Bacterial expression systems
InactiveUS20030224009A1Improve stabilityReduced expression levelHydrolasesProtozoaBacteroidesEscherichia coli
An inducible expression system is provided that includes an inducible ansB promoter co-dependently regulatable by cyclic AMP and anaerobiosis. The expression system is particularly suited to chromosomal expression of immunogenic proteins in attenuated bacterial vaccines. Protein expression from an E. coli-derived ansB promoter is particularly effective in a Salmonella host bacterium.
Owner:THE UNIV OF QUEENSLAND
Quadruple PCR detection primer group and kit for simultaneously detecting shigella, salmonella, clostridium welchii and escherichia coli
PendingCN112725478AGood economic valueHomologous relationship is smallMicrobiological testing/measurementMicroorganism based processesEscherichia coliSalmonella kiel
The invention discloses a quadruple PCR detection primer group for simultaneously detecting shigella, salmonella, clostridium welchii and escherichia coli, which comprises four pairs of primers, namely a shigella upstream primer pair and a shigella downstream primer pair which are respectively SEQIDNO: 1 and SEQIDNO: 2; an upstream primer pair and a downstream primer pair of salmonella are respectively SEQ ID NO: 3 and SEQ ID NO: 4; the clostridium welchii A type primer pair is shown as SEQ ID NO: 5 and SEQ ID NO: 6 respectively; and the primer pairs of the escherichia coli are respectively SEQ ID NO: 7 and SEQ ID NO: 8. A multiple detection method is established for common enteropathogenic bacteria in chicken farms, on the basis of high detection accuracy and good specificity, the detection time is shortened, the detection efficiency is improved, and the multiple detection method is more beneficial to application in large-scale breeding production.
Owner:青岛迪诺瓦基因科技有限公司 +2
Salmonella/Shigella/Staphylococcus aureus composite enriched medium and preparation method thereof
InactiveCN104195081ASimple ingredientsEasy to prepareBacteriaMicroorganism based processesStaphylococcus pseudintermediusMultiplex pcrs
The invention discloses a Salmonella / Shigella / Staphylococcus aureus composite enriched medium and a preparation method thereof. The medium comprises the following components in parts by mass: 5-15 parts of tryptone, 2.5-7.5 parts of yeast extract, 5-15 parts of sodium chloride, 1-3 parts of glucose, 1.5-3 parts of disodium hydrogen phosphate, 1-3 parts of mannitol, 1-3 parts of sodium pyruvate, 1-3 parts of glycine and 1000 parts of distilled water. The pH value is 7.2-7.5. The preparation method comprises the following steps: adding all the components to the distilled water, dissolving by stirring and autoclaving. The Salmonella, Shigella and Staphylococcus aureus are main pathogens in need of detection in national standard of food sanitation. The composite enriched medium can be utilized to implement constant-speed and high-speed proliferation of the Salmonella, Shigella and Staphylococcus aureus. After proliferation for 16 hours, the composite enriched medium can be directly used for high-flux detection of multiplex PCR (polymerase chain reaction), gene chips, microfluidic chips and the like to screen the three pathogens.
Owner:CAPITALBIO CORP +2
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