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56results about How to "High coupling rate" patented technology
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Preparation method of polystyrene fluorescent microsphere coupled with antibody
InactiveCN104530459AHigh coupling rateGood dispersionFluorescence/phosphorescenceAntiendomysial antibodiesPolystyrene
The invention discloses a preparation method of a polystyrene fluorescent microsphere coupled with an antibody, which comprises the following steps: 1. activating a carboxyl group on the surface of a fluorescent microsphere; 2. coupling an amino group of a monoclonal antibody with the carboxyl group on the surface of the fluorescent microsphere; 3. blocking activated groups not subjected to complete reaction on the surface of the fluorescent microsphere; and 4. preserving the polystyrene fluorescent microsphere coupled with the antibody. The preparation method disclosed by the invention is simple, easy to implement, low in cost and suitable for industrialization.
Owner:NANJING UNIV OF POSTS & TELECOMM
Polymer hydrogel based on Fe3+-dopamine modification and preparing method thereof
The invention discloses polymer hydrogel based on Fe3+-dopamine modification and a preparing method thereof. Polymer containing a hydroxyl group is used as a main raw material, dopamine is selected as a functional group, and p-nitrophenyl chlorate is used as an activating agent and directly reacts with dopamine hydrochloride to prepare the polymer with the terminal modified by dopamine; coordination of the polymer with the terminal modified by dopamine and Fe3+ through pH adjustment is achieved. The hydrogel has adhesiveness, thermosensitivity, pH responsiveness, self-healing and other multifunctional characteristics and has potential application value in biomedical engineering and underwater mechanical coatings.
Owner:SANMING UNIV
Aflatoxin B2 aptamer affinity column and preparation method and application thereof
ActiveCN108251427ASimple and efficient operationLow priceOrganic chemistryOther chemical processesFluorescenceChemistry
The invention provides an aflatoxin B2 aptamer affinity column and a preparation method thereof, wherein the affinity column uses cyanogens-bromide-modified agarose as a vector, a nucleic acid aptamercapable of recognizing aflatoxin B2 with high affinity and high specificity is covalently coupled to the vector, and the coupled aflatoxin B2 aptamer complex vector is loaded into the affinity column. The affinity column is mainly used for purification and cleaning of the aflatoxin B2 in food, feeds, milk, blood samples, traditional Chinese medicines and other various samples so as to facilitatehigh performance liquid chromatography and fluorescence detection of the aflatoxin B2 in the samples.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Preparation and application of hexahistidine-tagged protein immunoaffinity purification and enrichment column
InactiveCN103111094AStrong specificityEasy to identifyPreparing sample for investigationSolid sorbent liquid separationPolyclonal antibodiesPolyhistidine-tag
The invention provides an His-tagged recombinant protein immunoaffinity purification and enrichment column capable of efficiently and specifically enriching hexahistidine-tagged protein and a preparation method and application thereof. The provided immunoaffinity purification and enrichment column comprises an activated agarose filler coupled with an immunoaffinity-purified His-specific polyclonal antibody and a plastic column loaded with the immunoaffinity filler, wherein the His-specific polyclonal antibody coupled with the immunoaffinity filler is extracted by an immunoaffinity method. The immunoaffinity purification and enrichment column is prepared by loading the immunoaffinity filler into the special plastic column; and the immunoaffinity purification and enrichment column is high in efficiency and strong in specificity while enriching His-tagged recombinant protein, is mild in diluting conditions, can be repeatedly utilized and is an ideal material for separating, purifying and enriching the His-tagged recombinant protein.
Owner:NANNING LANGUANG BLUE LIGHT BIOTECH +1
Acapsular type staphylococcus aureus extracellular polysaccharide-protein conjugate and preparation method thereof
InactiveCN102120761AEasy to purifyQuality improvementDepsipeptidesPeptide preparation methodsStaphylococcus cohniiADAMTS Proteins
The invention relates to an acapsular type staphylococcus aureus extracellular polysaccharide-protein conjugate and a preparation method thereof. The acapsular type staphylococcus aureus extracellular polysaccharide-protein conjugate is prepared by performing covalent coupling on extracellular polysaccharide of acapsular type staphylococcus aureus and protein under the action of a coupling reagent. The preparation method is an antidiuretic hormone (ADH) bridge method or an l-ethyl-3(3-diaminopropyol)-carbodiimide (EDAC) zero-distance crosslinking method. The preparation of the acapsular type staphylococcus aureus extracellular polysaccharide-protein conjugate provides a strong technical support for development of a vaccine for preventing staphylococcus aureus.
Owner:新疆维吾尔自治区畜牧科学院兽医研究所
Pseudo graded-index optical focusing device
InactiveUS20190018197A1High coupling rateCoupling light guidesOptical waveguide light guideRefractive indexMode switching
The invention relates to an optical coupling device for coupling a first waveguide, for example a multi-mode waveguide, to a second waveguide, for example a single-mode waveguide. The device is formed in a core layer and comprises a focusing structure (SF) capable of converting a light beam from a target mode of the first waveguide to a target mode of the second waveguide. The focusing structure comprises a plurality of trenches (T1-T4) made in the core layer to create a pseudo graded refractive index, itself able to convert the light beam from the target mode of the first waveguide to the target mode of the second waveguide.The scope of the invention extends to a photonic circuit comprising a single-mode waveguide, a multi-mode waveguide and a device according to the invention for coupling the single-mode waveguide to the multi-mode waveguide. The multi-mode waveguide can comprise a surface coupling network in order to allow for coupling with an optical fibre.
Owner:COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
D-dimer detection kit and preparation method thereof
InactiveCN106405076AHigh detection sensitivityImprove bindingMaterial analysisImmune complex depositionLatex particle
The invention relates to the technical field of biology, and discloses a D-dimer detection kit and a preparation method thereof. The kit comprises a buffer solution, D-dimer, a coagulation accelerator, a stabilizing agent, and an antiseptic; wherein the coagulation accelerator is polyvinylpyrrolidone (PVP). By adding polyvinylpyrrolidone (PVP) taken as a coagulation accelerator into the D-dimer detection kit, the combination between an antigen and an antibody is promoted to form a compound rapidly, at the same time, the dissociation of immune complex is inhibited, the precipitate appears rapidly, the detection speed is accelerated; moreover, the non-specific range is not enlarged, and the wide linear range of the D-dimer detection kit is maintained. Furthermore, PVP can fix D-dimer monoclonal antibodies on latex particles, the change of turbidity is specifically enhanced, and thus the detection sensitivity of the kit is improved.
Owner:SHANGHAI KEHUA BIO ENG
Aflatoxin B1 and B2 aptamer affinity column and preparation method and use thereof
The invention provides an aflatoxin B1 and B2 aptamer affinity column and a preparation method thereof. The affinity column uses a cyanogen bromide-modified agarose as a carrier, then aptamers for high affinity and high specificity recognition of aflatoxin B1 and B2 and the carrier are covalently coupled, and the coupled aflatoxin B1 and B2 aptamer complex carrier fills the affinity column. The affinity column is mainly used for purification of aflatoxin B1 and B2 in multiple samples such as foods, feed, milk, blood samples and traditional Chinese medicines and is conducive to later high-performance liquid chromatography and fluorescence detection of aflatoxin B1 and B2 in the sample.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Linear functionalized SBS prepared from bifunctional coupling agent and process
ActiveCN105801784AIncrease polarityHigh coupling rateBuilding insulationsHydrocarbon solventsPolymer science
The invention discloses a linear functionalized SBS prepared from a bifunctional coupling agent. The linear functionalized SBS is prepared by the following steps: (1) adding a hydrocarbon solvent, styrene and a polar additive into a reactor, stirring, heating to 30-60 DEG C, and adding an organic lithium initiator to carry out first-section polymerization reaction for 30-60 minutes; (2) adding butadiene to carry out second-section polymerization reaction for 20-60 minutes; (3) adding the bifunctional coupling agent to carry out coupling reaction for 2-30 minutes; and (4) after the reaction in the step (3) is finished, adding a terminating agent to terminate the reaction, adding an anti-aging agent, removing the hydrocarbon solvent, and drying, so as to obtain the styrene-butadiene-styrene segmented ternary copolymer with linear functional groups. The invention further discloses a preparation process of the linear functionalized SBS. The linear functionalized SBS has good compatibility with polar materials such as asphalt, and the preparation process is simple.
Owner:CHINA PETROLEUM & CHEM CORP
Magnetic solid phase extraction material of aflatoxin B1 and B2, preparation method and application thereof
The invention provides a magnetic solid phase extraction material of aflatoxin B1 and B2, a preparation method and application thereof. The magnetic solid phase extraction material, by adopting superparamagnetic Fe3O4@SiO2 as a core and natural hydrophilic agarose as a matrix, encapsulates agarose on the surface of Fe3O4@SiO2 by a composite emulsification technique, and then modifies the NHS groupon the surface of the agarose, thereby being capable of binding to the aptamer by covalent coupling. Compared with traditional carboxyl and amino magnetic beads, magnetic agarose microspheres containing NHS groups on the surface do not need to be activated with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or glutaraldehyde, and the aptamer can be covalently coupled to the magnetic beads by mixing the aptamer solution with NHS magnetic agarose magnetic beads for 1 to 2 hours at room temperature. The magnetic solid phase extraction material provided by the invention is used as a novel magneticsolid phase extraction adsorbent for the detection and analysis of aflatoxins B1 and B2 in food, agricultural products and traditional Chinese medicine.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Method for preparing heavy metal holoantigen with high coupling rate and high activity
InactiveCN102875668AReduce damageHigh coupling rateOvalbuminSerum albuminPhysical chemistryCarrier protein
The invention discloses a method for preparing heavy metal holoantigen with high coupling rate and high activity. The method is implemented by improving the synthetic sequence of the heavy metal holoantigen and comprises the following steps of: reacting superfluous heavy metal ion with bifunctional chelator completely, regulating the pH value by using a NaOH solution; performing centrifugal precipitation; removing the superfluous heavy metal ion to obtain a metal ion and chelator compound, so that the metal ion and chelator compound cannot be well reacted with a biomacromolecule; coupling the metal ion and chelator compound with carrier protein, so that the activity of protein in the prepared holoantigen is guaranteed; and thus obtaining the holoantigen with high coupling rate and high activity. Immune tests in mice prove that the prepared holoantigen has high immunogenicity and is simple and practicable, and the preparation method has high repeatability.
Owner:NANCHANG UNIV
Preparation and application of hemagglutinin peptide mark recombinant protein immunoaffinity purification enriching column
InactiveCN103113455AStrong specificityEasy to identifySolid sorbent liquid separationPeptide preparation methodsHemagglutininPolyclonal antibodies
The invention provides a high-efficiency specificity enriched hemagglutinin peptide HA mark recombinant protein immunoaffinity purification enriching column as well as a preparation method and an application thereof. The immunoaffinity purification enriching column provided by the invention comprises an activated agarose filler coupled with HA specificity polyclonal antibody which is subjected to immunoaffinity purification and a plastic column carrying the immunoaffinity filler. The immunoaffinity purification filler coupled HA specificity polyclonal antibody is extracted by using an immunoaffinity method; and the immunoaffinity purification enrichment column is obtained by carrying the immunoaffinity filler into a specific plastic column. The immunoaffinity purification enrichment column enriched HA mark recombinant protein is high in efficiency, strong in specificity and temperate in elution condition, can be repeatedly utilized and is an ideal material for separating, purifying and enriching the HA mark recombinant protein at present.
Owner:NANNING LANGUANG BLUE LIGHT BIOTECH
Preparation method for umami peptide affinity column
ActiveCN105381631AHigh coupling rateLarge column capacitySolid sorbent liquid separationPeptide preparation methodsStationary phaseCombinatorial chemistry
The present invention discloses a preparation method for a umami peptide affinity column. The method comprises the following steps: synthesizing receptor proteins T1R1 and T1R3; taking protein T1R1 / T1R3 agarose gel as a solid phase support; coupling the protein T1R1 / T1R3 on the support with umami peptide; and chemically bonding the coupled umami peptide with a stationary phase. The preparation method for the affinity column is simple and reusable; purification efficiency is improved, so that the sensitivity of the method is improved; theoretically the method is applicable to various sample matrices; and due to the protein receptors T1R1 / T1R3, the method has the good separation effect on the umami peptide, high coupled specificity, a large adsorption amount and can be used repeatedly.
Owner:ZHEJIANG ACAD OF MEDICAL SCI
Antibody coupling type active targeting drug-loading long-circulating lipidosome and preparation method thereof
InactiveCN107998080AImprove bioavailabilityEliminate side effectsOrganic active ingredientsPharmaceutical non-active ingredientsChemotherapeutic drugsAnti-Tumor Drugs
The invention discloses an antibody coupling type active targeting drug-loading long-circulating lipidosome and a preparation method thereof. The targeting long-circulating lipidosome comprises an antibody, a chemotherapeutic drug and a lipidosome body, wherein the antibody is anti-angiogenic endothelia growth factor monoclonal antibody; the chemotherapeutic drug is oxaliplatin; the long-circulating lipidosome body comprises phospholipid, cholesterol and distearoyl phosphoethanolamine-polyethylene glycol 2000; the antibody is coupled with oxaliplatin lipidosome and prepared through the raw materials including phospholipid, cholesterol, distearoyl phosphoethanolamine-polyethylene glycol 2000, novel anti-angiogenic endothelia growth factor monoclonal antibody (anti-VEGF monoclonal antibody)and antitumor drug that is oxaliplatin. The primary result is obtained at present and shows that the antibody coupling rate is 43.76%; the activity remaining rate of the antibody is 84.16%. Therefore,the active targeting lipidosome can be effectively and specifically combined with vascular endothelial growth factor, as a result, the toxicity reducing and efficacy enhancing effects can be achieved.
Owner:SOUTHEAST UNIV
Preparation method of quantum dots-antibody immunocomplex
ActiveCN108918858AHigh fluorescence intensityStrong fluorescence Strong biological activityFluorescence/phosphorescenceThio-Quantum dot
The invention a preparation method of quantum dots-antibody immunocomplex, including the steps of: 1) performing a reaction to carboxylic-modified quantum dots with an activator at 0-10 DEG C in a buffer solution with pH value being 5-6 to obtain activated quantum dots, wherein the activator includes 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide or a thio-substance thereof; 2) performing a reaction to the activated quantum dots with an antibody at 0-10 DEG C in a buffer solution with pH value being 6-9, thus preparing the quantum dots-antibody immunocomplex. The immunocomplex has strong fluorescence intensity, bioactivity and like features and can reach about 90% in coupling rate. The preparation method is greatly improved in raw material utilizationrate and is beneficial to large-scale application.
Owner:RES INST OF XIAN JIAOTONG UNIV & SUZHOU
Nanoscale single exosome separation method
ActiveCN111518742AEasy accessHigh Separation YieldCell dissociation methodsElectrical/wave energy microorganism treatmentMagnetic sourceMagnetic bead
The invention discloses a nanoscale single exosome separation method. The method comprises the following steps of fully diluting a cell culture solution containing exosome; adding a superparamagneticamino magnetic bead diluent, and carrying out low-frequency oscillation mixing until the nano magnetic beads are coupled with the exosome; transferring a coupling solution to a surface layer of a nanopore array microchip; placing the microchip on a shaking table, and oscillating in low speed and small angle, so that the coupling solution is uniformly distributed on the surface layer of the chip; placing a magnetic source on the lower surface of the microchip, and enabling a magnetic bead exosome couplet to enter a nanopore array by using a progressive magnetic adsorption mode; under the actionof larger magnetic force, maintaining stable adsorption of the magnetic bead exosome couplet, cleaning the upper surface of a substrate with a buffer solution, and standing to obtain the nano microchip containing the single exosome which can be directly used for subsequent detection. On the basis of the nanopore microarray microchip with the high-precision screening effect, high-purity single exosome can be obtained through magnetic bead coupling exosome and progressive magnetic adsorption.
Owner:XI AN JIAOTONG UNIV
Vomitoxin and derivative aptamer affinity column and preparation method and application thereof
ActiveCN109833648ASimple and fast operationLow priceOther chemical processesPreparing sample for investigationDeoxynivalenol-3-glucosideN-Hydroxysuccinimide
The invention provides vomitoxin and a derivative aptamer affinity column and a preparation method and application thereof. The affinity column is prepared by the following steps: using N-hydroxysuccinimide modified agarose as a carrier, then carrying out covalent coupling on the carrier and a nucleic acid aptamer capable of high-affinity and high-specificity recognition of deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl deoxynivalenol and 15-acetyl deoxynivalenol in a sample, and finally filling an affinity column with the coupled aptamer composite carrier. The affinity column is mainlyused for purification and decontamination of vomitoxin and its derivative in food, feeds, milk, food crops, traditional Chinese medicines and other various samples, which is beneficial to high performance liquid chromatography and rapid detection of toxins in the sample in later period. By one-time loading of the column, four types of toxins can be purified and enriched, the number of reuse can reach 15 times, recovery rate is 80% and above, the cost is reduced, and the sample pre-treatment efficiency of the sample is enhanced.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Ofloxacin-hemocyanin coating antigen and preparation method thereof and detection test card
InactiveCN106749632AHigh coupling rateReactogenicBiological material analysisDepsipeptidesMedicineBiological activation
The invention provides an ofloxacin-hemocyanin coating antigen and a preparation method thereof. The structure of the ofloxacin-hemocyanin coating antigen is shown in the description. The preparation method of the ofloxacin-hemocyanin coating antigen includes the steps that C-chain derivatization is conducted on carboxyl of ofloxacin, activation is conducted on KLH at the same time, an acid-anhydride coupling method is adopted to synthesize the coating antigen, and the prepared coating antigen has the advantages of high coupling efficiency and reactogenicity. The invention further provides a test card for testing the ofloxacin, the test card is fast in detection and high in specificity and sensitivity, the detection limit of the test card can reach 0.06 ng / ml, and the requirements of rapid detection are met at the same time.
Owner:HENAN INST OF SCI & TECH
Seafood product detection kit and preparation method thereof
ActiveCN111398582AHigh activityIncrease the amount of retouchingMaterial analysisBiotechnologyCarboxyl radical
The invention provides a seafood product detection kit and a preparation method thereof, and belongs to the field of microbiological detection. The seafood product detection kit comprises anti-vibrioparahaemolyticus immunomagnetic beads and CdSe / ZnS-carboxylated polystyrene immunofluorescent microspheres. The magnetic beads provided by the invention are relatively high in surface carboxyl modification amount, the coupling rate of antibodies can be increased, and the capture rate of vibrio parahaemolyticus can be increased. According to the preparation method of the CdSe / ZnS-carboxylated polystyrene immunofluorescent microspheres, the cross-linking polymerization of the fluorescent microspheres is reduced, the dispersity is improved, the coupling rate of an antibody is improved, and the fluorescence intensity value is increased. The seafood product detection kit provided by the invention is used for detecting the vibrio parahaemolyticus and has relatively high sensitivity, accuracy andreliability.
Owner:ZHEJIANG OCEAN UNIV
Preparation method of immunoaffinity column
InactiveCN106908597AHigh coupling rateHigh specific adsorptionComponent separationSulfur drugSulfadiazine
The invention discloses a preparation method of an immunoaffinity column. The immunoaffinity column is a compound affinity column which is mainly prepared from three types of immunoaffinity columns through mixed packing in a volume ratio being 1:1:1, and the three types of immunoaffinity columns are prepared from three antibodies and a coupling matrix through coupling and wet packing. The column capacity of the immunoaffinity column is 2,500 ng, the immunoaffinity column is good in purification effect and can be repeatedly used three times, and average recovery rate is 67%-107%. The compound immunoaffinity column has good specific adsorption property for at least 12 sulfonamides including sulfaquinoxaline, sulfadiazine, sulfapyridine, sulfametoxydiazine, sulfadimidine, sulfamethoxazole, sulfafurazole, sulfadimethoxypyrimidine, sulfathiazole, sulfamerazine, sodium N-sulphanilamidate and sulfamonomethoxine. The immunoaffinity column which is high in coupling rate, specific adsorption property, column capacity and recovery rate is prepared, and a sensitive, rapid and simple analysis method capable of detecting residues of the 12 sulfonamides simultaneously is provided.
Owner:花锦
Dynamic random access memory cell and method for fabricating the same
ActiveUS20050202628A1Small sizeSimple manufacturing processTransistorSolid-state devicesDielectric layerCapacitor
A DRAM cell and a method for fabricating the same are provided. The method includes: forming a trench in a substrate; forming a first capacitor dielectric layer on the surface of the trench; forming a conducting layer inside the trench; forming a second capacitor dielectric layer on the surface of the substrate and on the conducting layer, wherein the substrate around the first and second capacitor dielectric layers serves as a bottom electrode; forming a protruding electrode on the substrate, the protruding electrode being on the substrate around the trench and covering a junction between the trench and the substrate; and electrically connecting the protruding electrode and the conducting layer, the conducting layer and the protruding electrode being an upper electrode.
Owner:MARLIN SEMICON LTD
Lactoferrin aptamer affinity column as well as preparation method and application thereof
ActiveCN112114074ASimple and fast operationLow priceComponent separationBiological material analysisAptamerOrganic chemistry
The invention provides a lactoferrin aptamer affinity column as well as a preparation method and application thereof. The affinity column takes agarose modified by N-hydroxysuccinimide as a carrier, anucleic acid aptamer capable of recognizing lactoferrin with high affinity and high specificity is covalently coupled with the carrier, and a solid-phase extraction column is filled with a specific affinity filler after coupling. The affinity column is mainly applied to specific recognition and enrichment purification of lactoferrin in a sample, the natural activity of lactoferrin can still be kept after purification, the method is rapid, simple, convenient and accurate, the affinity column can be repeatedly used, and the affinity column can be used for sample pretreatment before detection ofa large instrument and can also be used for separation and purification of lactoferrin. Wide application prospects are realized in the fields of cosmetics, food, animal production, medical treatmentand the like.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Preparation method for immunomagnetic microsphere, Sudan red detection kit containing immunomagnetic microsphere and detection method
The invention provides a preparation method for an immunomagnetic microsphere. The method adopts a phosphate buffer containing 0.01%-0.1% of Tween-20 and 0.01%-0.1% of polyvinylpyrrolidone as the activating buffer and / or coupling buffer. The invention also discloses a Sudan red detection kit containing the immunomagnetic microsphere and a detection method using the kit to conduct detection. The immunomagnetic microsphere preparation method provided by the invention has obviously improved coupling rate, and the obtained immunomagnetic microsphere has good stability. The kit provided by the invention uses the Sudan red monoclonal antibody coated immunomagnetic microsphere, has high sensitivity, can be used for rapid screening of mass samples, can avoid the influence of the matrix in a to-be-detected sample, and has high accuracy. The detection method provided by the invention can play an important role in food safety detection, and has important social significance and broad market prospects.
Owner:BEIJING PURKINJE GENERAL INSTR
Preparation and application of flag peptide tagged recombinant protein immunoaffinity purification and enrichment column
InactiveCN103111093AStrong specificityEasy to identifySolid sorbent liquid separationPeptide preparation methodsPolyclonal antibodiesFLAG peptide
The invention provides a D-tag tagged recombinant protein immunoaffinity purification and enrichment column capable of efficiently and specifically enriching flag peptide tagged protein and a preparation method and application thereof. The provided immunoaffinity purification and enrichment column comprises an activated agarose filler coupled with an immunoaffinity-purified D-tag specific polyclonal antibody and a plastic column loaded with the immunoaffinity filler, wherein the D-tag specific polyclonal antibody coupled with the immunoaffinity filler is extracted by an immunoaffinity method. The immunoaffinity purification and enrichment column is prepared by loading the immunoaffinity filler into the special plastic column; and the immunoaffinity purification and enrichment column is high in efficiency and strong in specificity while enriching D-tag tagged recombinant protein, is mild in diluting conditions, can be repeatedly utilized and is an ideal material for separating, purifying and enriching the D-tag tagged recombinant protein.
Owner:NANNING LANGUANG BLUE LIGHT BIOTECH
A kind of enterohaemorrhagic Escherichia coli o157:h7 latex agglutination detection kit and its application
ActiveCN104849470BEasy to operateReduce testing costsBiological testingEscherichia coliPositive control
Owner:广东正大康生物科技股份有限公司
Alternariol aptamer affinity column and its preparation method and application
Provided are an alternariol aptamer affinity column and a preparation method and an application therefor. The affinity column uses agarose modified with N-hydroxysuccinimide as a carrier, and then covalently couples to the carrier a nucleic acid aptamer capable of recognising alternariol with high affinity and high specificity, the coupled product being used as a filler for packing the affinity column.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
A preparation method of immunomagnetic microspheres, and a Sudan red detection kit and detection method containing immunomagnetic microspheres
The invention provides a preparation method for an immunomagnetic microsphere. The method adopts a phosphate buffer containing 0.01%-0.1% of Tween-20 and 0.01%-0.1% of polyvinylpyrrolidone as the activating buffer and / or coupling buffer. The invention also discloses a Sudan red detection kit containing the immunomagnetic microsphere and a detection method using the kit to conduct detection. The immunomagnetic microsphere preparation method provided by the invention has obviously improved coupling rate, and the obtained immunomagnetic microsphere has good stability. The kit provided by the invention uses the Sudan red monoclonal antibody coated immunomagnetic microsphere, has high sensitivity, can be used for rapid screening of mass samples, can avoid the influence of the matrix in a to-be-detected sample, and has high accuracy. The detection method provided by the invention can play an important role in food safety detection, and has important social significance and broad market prospects.
Owner:BEIJING PURKINJE GENERAL INSTR
Polymer hydrogel modified based on fe3+-dopamine and its preparation method
The invention discloses polymer hydrogel based on Fe3+-dopamine modification and a preparing method thereof. Polymer containing a hydroxyl group is used as a main raw material, dopamine is selected as a functional group, and p-nitrophenyl chlorate is used as an activating agent and directly reacts with dopamine hydrochloride to prepare the polymer with the terminal modified by dopamine; coordination of the polymer with the terminal modified by dopamine and Fe3+ through pH adjustment is achieved. The hydrogel has adhesiveness, thermosensitivity, pH responsiveness, self-healing and other multifunctional characteristics and has potential application value in biomedical engineering and underwater mechanical coatings.
Owner:SANMING UNIV
Preparation and Application of Hexahistidine-tagged Protein Immunoaffinity Purification Enrichment Column
InactiveCN103111094BStrong specificityEasy to identifyPreparing sample for investigationSolid sorbent liquid separationPolyclonal antibodiesPolyhistidine-tag
The invention provides an His-tagged recombinant protein immunoaffinity purification and enrichment column capable of efficiently and specifically enriching hexahistidine-tagged protein and a preparation method and application thereof. The provided immunoaffinity purification and enrichment column comprises an activated agarose filler coupled with an immunoaffinity-purified His-specific polyclonal antibody and a plastic column loaded with the immunoaffinity filler, wherein the His-specific polyclonal antibody coupled with the immunoaffinity filler is extracted by an immunoaffinity method. The immunoaffinity purification and enrichment column is prepared by loading the immunoaffinity filler into the special plastic column; and the immunoaffinity purification and enrichment column is high in efficiency and strong in specificity while enriching His-tagged recombinant protein, is mild in diluting conditions, can be repeatedly utilized and is an ideal material for separating, purifying and enriching the His-tagged recombinant protein.
Owner:NANNING LANGUANG BLUE LIGHT BIOTECH +1
Lactoferrin aptamer affinity column and its preparation method and application
ActiveCN112114074BSimple and fast operationLow priceComponent separationBiological material analysisAptamerOrganic chemistry
The invention provides a lactoferrin aptamer affinity column as well as a preparation method and application thereof. The affinity column uses N-hydroxysuccinimide-modified agarose as a carrier, and covalently couples a nucleic acid aptamer capable of recognizing lactoferrin with high affinity and specificity to the carrier. After coupling, the specific affinity and packed solid phase extraction column. The affinity column is mainly used for the specific identification and enrichment of lactoferrin in the sample, and the natural activity of lactoferrin can still be maintained after purification. The method is fast, simple and accurate, and the affinity column can be used repeatedly. It can also be used for the separation and purification of lactoferrin in the sample pretreatment before the detection of large instruments, and has broad application prospects in the fields of cosmetics, food, animal production, and medical treatment.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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