34 results about "Hemocyanin" patented technology

Uses are described for hemolymph, contents of hemolymph, fragments or components of the contents found within hemolymph, including hemocyanin, any of which may be derived from crustaceans, or recombinantly produced, as functional agents to treat diseases, provide symptomatic relief, provide a beautifying or relieving effect to the intended organ or site-of-intended-use, or be used as an ingredient in formulations designed to help regenerate, repair, or restore the function of the skin barrier, for applications falling within skin barrier restoration, wound healing and anti-aging effects.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The application relates to a hapten for detecting ethoxyquin, a complete antigen and a use method thereof. The application discloses a hapten for detecting ethoxyquin, and then a complete antigen obtained by coupling ethoxyquin hapten with hemocyanin is used as an immunogen, and a complete antigen obtained by coupling ethoxyquin hapten with chicken egg white albumin is used as a coating agent. An indirect detection enzyme-linked immunoassay method for ethoxyquin is established. The half-inhibition concentration of the method for ethoxyquin is 0.96 ug / mL, the detection limit is 0.6 ug / mL, the linear detection range is 0.72 ug / mL-1.28 ug / mL, and the curve correlation coefficient is 0.996. The method is high in specificity and sensitivity, simple and fast, and has a wide application prospect in the detection of ethoxyquin residues in food. The method is very suitable for realizing rapid qualitative or quantitative detection of ethoxyquin in food by primary site personnel.

The application discloses application of shrimp hemocyanin in a carrier protein of a hapten, and belongs to the technical field of biological medicines. Compared with KLH commonly used in the prior art, the shrimp hemocyanin provided in the application has good solubility, the whole antigen obtained by coupling the hapten has good solubility and is not easy to precipitate and separate out, and the immunogenicity of the shrimp hemocyanin is better than that of KLH; the whole antigen prepared by taking the shrimp hemocyanin as a carrier protein has better animal immunization activity than the whole antigen prepared by taking KLH as a carrier protein. The shrimp hemocyanin provided in the application is extracted from blood of prawns, the prawn breeding technology is mature, the shrimp hemocyanin is easy to obtain, and the purchase cost is low, so that the preparation cost of the shrimp hemocyanin is far lower than the purchase cost of KLH.

The invention discloses a duck GRPR polypeptide and a preparation method and application of a polyclonal antibody of the duck GRPR polypeptide, and belongs to the field of biochemistry and molecular immunology. The duck GRPR gene is cloned for the first time, and the structure of the duck GRPR gene is analyzed; the preparation method comprises the following steps: taking amino acid polypeptide at 350-368 sites of a carboxyl terminal of duck GRPR protein as a hapten, and carrying out N-terminal modification on a polypeptide sequence to obtain duck GRPR modified polypeptide; and coupling the modified polypeptide with a carrier protein keyhole limpet hemocyanin to prepare a complete antigen, immunizing an animal, and separating and purifying from antiserum to obtain the polyclonal antibody of the duck GRPR polypeptide. Experiments show that the polyclonal antibody can be specifically combined with GRPR protein in duck tissue. According to the invention, the blank in the field of duck GRPR protein detection and research is filled, and a foundation is laid for research of the functions of the GRPR in the duck body.

The present application relates to a rabbit polyclonal antibody targeting human PI3K beta tyrosine phosphorylation at position 962, and a preparation method and application thereof. The present application first identifies that PI3K beta protein Y962 site is phosphorylated and modified and the phosphorylation modification of the site significantly affects the function of PI3K beta protein in PTEN knockout cells through mass spectrometry combined with a series of molecular biology experiments, so an antigen peptide containing Y962 site phosphorylation is synthesized, an immunogen is obtained by coupling a carrier protein hole hemocyanin, a Japanese white rabbit is immunized six times combined with an adjuvant, and a PI3K beta Y962 site specific phosphorylation antibody is obtained after purification and identification. The present application can specifically detect the PI3K beta Y962 site phosphorylation level in cells and tissue samples, and provides an important tool for studying the role and mechanism of PI3K beta phosphorylation in tumors.

The invention provides a hybridoma cell strain secreting a toxoflavin monoclonal antibody. The hybridoma cell strain is obtained by immunogen immune animals prepared by coupling toxoflavin hapten and carrier protein. Wherein the carrier protein for preparing the immunogen comprises keyhole limpet hemocyanin KLH. The hybridoma cell strain is preserved in the Guangdong Microbial Culture Collection Center on March 10, 2026, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.67912. The invention also provides an application of the hybridoma cell strain, specifically, the toxoflavin monoclonal antibody secreted by the hybridoma cell strain is applied to toxoflavin detection. The kit further comprises a coating antigen, the coating antigen is prepared by coupling the toxoflavin hapten and carrier protein, and the carrier protein for preparing the coating antigen comprises bovine serum albumin (BSA). The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to toxoflavin, and can be accurately and reliably used for quantitative detection of toxoflavin in an actual sample.

The invention belongs to the technical field of biological medicines, and particularly relates to a human NSUN2 protein 190th lysine site lactic acid antigen, an antibody, a preparation method and application thereof. Relates to the technical field of lactylated antibody preparation, and the preparation method comprises the following steps: S01, carrying out lactylation on 190th lysine in a polypeptide of an amino acid sequence, and coupling with a carrier protein to obtain an initial antigen, the carrier protein comprising hemocyanin KLH; s02, injecting the obtained antigen into a living body culture sample for immune culture, and collecting whole blood after a preset period to obtain multi-antibody serum; and S03, purifying to obtain an iterative antibody. According to the invention, the expression difference in sensitive and drug-resistant tumor cells and tumor tissues can be detected by directly applying Western Blotting, immunofluorescence and immunohistochemistry, so that a powerful guarantee is provided for the detection of tumor sensitivity.

The invention relates to an anti-lavender monoclonal antibody and application of the anti-lavender monoclonal antibody in detection of lavender honey. The monoclonal antibody is prepared by the following steps: immunizing a Balb / c mouse by using a linalool-KLH conjugate as an immunogen, and carrying out cell fusion, hybridoma screening and ascites purification, so as to obtain the monoclonal antibody. The sequence of a heavy chain variable region is SEQ ID NO: 1, the sequence of a light chain variable region is SEQ ID NO: 5, and the affinity constant (Ka) reaches 1.25 * 10 < 9 > L / mol. The antibody has a linalool sensitivity of 1.05-3.63 [mu] g / mL in competitive ELISA detection, and can specifically distinguish lavender honey (the inhibition rate is greater than or equal to 40%) from other honey source honey (the inhibition rate is less than or equal to 10%). The lavender honey immunodetection method based on the monoclonal antibody is established for the first time, has the advantages of being high in specificity, easy and convenient to operate and low in cost, and is suitable for rapid screening of authenticity of honey products.

Uses are described for hemolymph, contents of hemolymph, fragments or components of the contents found within hemolymph, including hemocyanin, any of which may be derived from crustaceans, as functional agents to treat diseases, provide symptomatic relief, provide a beautifying or relieving effect to the intended organ or site-of-intended-use, or be used as an ingredient in formulations designed to aid in the relief of pathologies or states commonly associated with inflammation and dry skin, including, but not limited to eczema, dermatitis, psoriasis, cystitis, asthma, sinusitis, joint pain, as well as other instances of acute and chronic inflammation, and for relief of general symptoms that may be associated with inflammation and dry skin but not linked directly in the intended use, including, but not limited to, redness, itchiness, irritation, muscle or joint fatigue, swelling, hydrating the skin, and reducing transepidermal water loss (TEWL).

The invention discloses a duck NMBR polypeptide, a polyclonal antibody and application, and belongs to the field of biochemistry and molecular immunology. The duck NMBR gene is cloned for the first time, and the structure of the duck NMBR gene is analyzed; amino acids at 341-359 sites at the carboxyl terminal of duck NMBR protein are used as haptens, the haptens are coupled with keyhole limpet hemocyanin to prepare complete antigens, then animals are immunized, and the polyclonal antibody of the duck NMBR polypeptide is separated and purified from antiserum. Experiments show that the polyclonal antibody can specifically bind to NMBR protein in duck tissue. According to the invention, the blank in the field of duck NMBR protein detection and research is filled, and a foundation is laid for research of the function of the NMBR in the duck body.

The application discloses trifluralin hapten, complete antigen, artificial antigen and antibody and a preparation method and application thereof, and relates to the technical field of food safety detection. The trifluralin hapten provided by the application almost retains the complete structure of trifluralin, and further has a carboxyl group which can react with bovine serum albumin, human serum albumin, hemocyanin and egg white albumin, so that the trifluralin hapten can be coupled with different carrier proteins to be used as artificial immunogens and coating origins, and then the trifluralin detection antibody is prepared, which is more conducive to stimulating the animal immune response to generate an antibody with stronger specificity and higher sensitivity. The trifluralin antibody has high sensitivity and good specificity, and can be used for rapidly detecting the residual trifluralin in animal food.

The application relates to a monoclonal antibody of alpha, beta and gamma amatoxin and a preparation method and application thereof. The monoclonal antibody of alpha amatoxin is prepared from an alpha amatoxin artificial antigen, the monoclonal antibody of beta amatoxin is prepared from a beta amatoxin artificial antigen, and the monoclonal antibody of gamma amatoxin is prepared from a gamma amatoxin artificial antigen; the alpha amatoxin artificial antigen is a conjugate of alpha amatoxin and keyhole limpet hemocyanin, the beta amatoxin artificial antigen is a conjugate of beta amatoxin and bovine serum albumin, and the gamma amatoxin artificial antigen is a conjugate of gamma amatoxin and human serum albumin. The three antibodies have a small cross-reaction rate, can simultaneously detect and distinguish alpha-AMA, beta-AMA and gamma-AMA in a sample, and have high accuracy.

The invention provides a kit for detecting endotoxin and a detection method thereof. The kit comprises: a capture unit: a recombinant crab-like hemocyanin factor C fixed on the surface of a solid phase carrier; the signal probe is composed of a polyclonal anti-lipopolysaccharide antibody coupled with gold nanoparticles and horse radish peroxidase; the reaction buffer solution is a Tris-HCl buffer solution containing calcium ions required by activation of Factor C and a surfactant; the signal substrate solution is a peroxidase chromogenic substrate solution containing 3, 3 ', 5, 5'-tetramethyl benzidine; the stop solution comprises a dilute sulphuric acid solution and an endotoxin standard substance. The rapid, high-sensitivity and high-specificity endotoxin detection kit based on the recombinant factor C and the nano signal amplification technology has the advantages of high specificity, interference resistance, high sensitivity, rapidness, convenience and the like, and is wide in application prospect.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention provides a novel streptococcus group A oligosaccharide antigen and glycobinding protein vaccine, which is characterized in that oligosaccharide antigen containing 30-70% of N-acetyl glucamine multi-rhamnose core skeleton structure is accurately prepared through chemical synthesis, and is efficiently coupled with KLH (the loading capacity is about 215 oligosaccharide / KLH molecules); safe, efficient and broad-spectrum immune protection is realized; safety: the GlcNAc side chain modification design avoids autoimmune risks (such as acute rheumatic fever heat); high efficiency: the animal experiment antibody titer reaches 1: 12, 800; the broad spectrum is as follows: the highly conservative universal GAS oligosaccharide antigen can universally cover various serotypes, and the key synthetic route (Scheme 2, 3, 16 and 20, the yield is 22%-96%) and the loading capacity verification (HPAEC) support the technical feasibility.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-6D6 and can be expressed through a recombinant Expi293 cell of a recombinant plasmid pcDNA3.1 containing a monoclonal antibody gene for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The application discloses a New Delhi tomato leaf curl virus coat protein polypeptide, antigen, antibody and application thereof, and belongs to the technical field of organism immunity detection. The application is based on ToLCNDV CP to design a polypeptide sequence, and provides a New Delhi tomato leaf curl virus coat protein polypeptide. The antigen prepared by coupling the polypeptide with keyhole limpet hemocyanin (KLH) can obtain a New Delhi tomato leaf curl virus antibody with high sensitivity and specificity, and can be used for screening the virus, and realizes instant detection and early warning of the ToLCNDV virus. Meanwhile, the application provides a theoretical basis and technical support for establishment of a virus serological diagnosis method and related detection product research and development, and is helpful to development of more accurate, efficient and portable detection products, so as to better serve the virus monitoring, quarantine and prevention and control work.

The application discloses a polystyrene micro-nano plastic immunochromatography time-resolved fluorescence kit and application thereof. The kit comprises a fluorescence test strip and a sample reaction bottle containing quantum dot fluorescence microsphere labeled anti-polystyrene monoclonal antibody freeze-dried product. The fluorescence test strip comprises a bottom plate, the adhesive surface of the bottom plate is sequentially pasted with a water absorption pad, a detection pad and a sample pad from top to bottom, adjacent pads are connected through overlapping connection at a connecting position, the detection pad takes a nitrocellulose membrane as a base pad, a transverse quality control line and a detection line are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with anti-mouse polyclonal antibody, and a polystyrene-hemocyanin conjugate is coated on the detection line. The immunochromatography time-resolved fluorescence kit provided by the application can realize quantitative detection of polystyrene, has the advantages of high specificity, high sensitivity, simple sample pretreatment, small environmental pollution hazard and suitability for on-site batch detection.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-14B3, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits of Chinese shrimp hemocyanin with molecular weights of 73 kDa and 75 kDa, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention discloses a yellow-blue discoloration hemocyanin mancozeb compound bactericide and a preparation method thereof, and belongs to the technical field of pesticide preparation, the yellow-blue discoloration hemocyanin mancozeb compound bactericide comprises, by mass, 30%-80% of mancozeb, 0.0001%-1% of hemocyanin, 2%-8% of supramolecular carriers and 4%-65% of auxiliaries, the compound bactericide is yellow, and the compound bactericide becomes blue when making contact with water. According to the invention, mancozeb and hemocyanin are inlaid through the supramolecular carrier, so that efficient synergy of biological protein and a chemical bactericide is realized, same-effect prevention and treatment are realized under the condition that the dosage of mancozeb is reduced, and the pollution to the environment is reduced; the stability of biological protein is improved, and the lasting period of the compound bactericide is prolonged; multi-stage targeted delivery is realized, so that the permeability of the bactericide to crop leaf surfaces is improved, and meanwhile, the drug resistance risk of crops to the bactericide is also reduced; meanwhile, the specific oxygenation discoloration characteristic of the hemocyanin is utilized, so that the drug effect of the sterilization component is visually monitored.

The application discloses a kind of anti-swine PCV4 monoclonal antibodies, it is characterized in that, based on PCV4 specific protein sequence synthesis sequence PCV4-C157, wherein the PCV4-C157 sequence is KPQDPSGETHTLHFQP;Synthetic sequence is coupled with inert protein hemocyanin after injection immunization is carried out to animal, after immunization, serum is collected, antibody in serum is extracted, and specific antibody is prepared, and the monoclonal antibody prepared based on the specific polypeptide fragment in PCV4 without cross reaction with PCV1, PCV2, PCV3 is used as detection approach, PCV4 virus can be quickly identified, simple operation, short detection time and low cost, the provided detection pig PCV4 antigen colloidal gold test strip is strong in specificity, high in sensitivity, accurate in result, does not need any instrument and equipment, can be operated on site, and does not need to be operated by professional personnel.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-1A1, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The present invention discloses a truncated polypeptide of carp herpesvirus type II and its application in the preparation of a carp herpesvirus type II vaccine. The truncated polypeptide CyHV‑2 ORF104 is a linear polypeptide of 9 amino acids in length, which is shown in SEQ ID NO: 1. The present invention obtains a CyHV‑2 ORF104 polypeptide vaccine by cross-linking a synthesized linear peptide segment with keyhole limpet hemocyanin (KLH) and aseptically treating it, and then mixing it with an adjuvant in proportion. The vaccine is intraperitoneally injected into gilded crucian carp, and it is found that the vaccine can effectively improve the survival rate of gilded crucian carp after challenge with the virus. Safety tests confirm that there are no adverse reactions after immunization of gilded crucian carp, and the vaccine can effectively prevent and treat CyHV‑2 infection in gilded crucian carp.

The present application relates to the field of aquatic animal disease-resistant breeding, and discloses a method for multi-index combined detection of the anti-DIV1 virus ability of Macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof, which comprises the following steps: after the Macrobrachium rosenbergii is anesthetized and disinfected, abdominal limb samples are obtained by minimally invasive cutting of abdominal limbs, and the live body is placed in water for recovery; total RNA is extracted and reversely transcribed into a cDNA template; specific primers are used for fluorescence quantitative PCR detection of chitinase 1C gene, C-type lectin domain-containing protein gene, hemocyanin gene, actin gene and an internal reference gene; and the relative expression data are standardized, directionally corrected, weighted and converted to obtain a comprehensive disease resistance index and a determination result. The present application obtains samples by minimally invasive cutting of abdominal limbs, ensures the survival and recovery of the live body, facilitates the in-vivo breeding of excellent individuals, and improves the accuracy and stability of detection and evaluation by combining multi-dimensional target genes for joint detection.

The invention discloses a synthesis method of a uniform sialylated AFP-L3 antigen, serum polyclonal antibody preparation and application thereof, and belongs to the field of immunology, the uniform sialylated AFP-L3 antigen comprises a sialylated AFP-L3 glycopeptide antigen or a sialylated AFP-L3 glycoprotein antigen, and the synthesis method comprises the following steps: transferring a carbohydrate chain to a starting substrate by using Endo-M N175Q transglycosylation reaction to obtain an intermediate I; wherein the starting substrate comprises a glycopeptide Fmoc-ATKVN (GlcNAc) FTEAQKAALDV-NH2 or an AFP (Alpha Fetal Protein) protein which is expressed in HEK293T and is subjected to hydrolysis treatment; modifying the intermediate I through fucose transferase FuT8 to obtain an intermediate II; the intermediate II is modified and extended through galactose transferase GalT and sialyltransferase SiaT, and an intermediate III or a sialylated AFP-L3 glycoprotein antigen is obtained; the sialylated AFP-L3 glycopeptide antigen is obtained through the steps that the sialylated AFP-L3 glycopeptide antigen is obtained, the intermediate III is subjected to Fmoc removal treatment and then conjugated with bis-succinimide glutaric acid ester and hemocyanin, and the serum polyclonal antibody generated based on the antigen has high titer and specificity.

The anti-porcine PCV4 monoclonal antibody disclosed by the invention is characterized in that a sequence PCV4-C157 is synthesized on the basis of a PCV4 specific protein sequence, and the sequence of the PCV4-C157 is KPQDPSGETHTLHFQP; a synthetic sequence and inert protein hemocyanin are coupled and then subjected to animal injection immunization, serum is collected after immunization, an antibody in the serum is extracted, a specific antibody is prepared, the monoclonal antibody prepared on the basis of specific polypeptide fragments which have no cross reaction with PCV1, PCV2 and PCV3 in PCV4 serves as a detection way, the PCV4 virus can be rapidly identified, operation is easy, detection time is short, cost is low, and the method is suitable for large-scale popularization and application. The colloidal gold test strip for detecting the porcine PCV4 antigen is high in specificity, high in sensitivity and accurate in result, does not need any instrument and equipment, can be operated on site, and does not need to be operated by professionals.

This invention discloses a method for preparing and applying antibodies modified by lactation of lysine at position 131 of human PGK1, relating to the field of lactated antibody preparation technology. The method includes the following steps: S01, lactating lysine at position 131 of a polypeptide and conjugating it with a carrier protein to obtain an initial antigen, wherein the carrier protein includes hemocyanin KLH; S02, injecting the obtained antigen into a live culture sample for immunoculture, collecting whole blood after a predetermined period to obtain polyclonal antibody serum; S03, purifying the antigen to obtain an iterative antibody. This invention can directly apply Western blotting, immunofluorescence, and immunohistochemistry to detect expression differences in normal cells, tumor cells, and tumor tissues, thus providing a strong guarantee for tumor detection.