17 results about "Hemocyanin" patented technology

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The application relates to a hapten for detecting ethoxyquin, a complete antigen and a use method thereof. The application discloses a hapten for detecting ethoxyquin, and then a complete antigen obtained by coupling ethoxyquin hapten with hemocyanin is used as an immunogen, and a complete antigen obtained by coupling ethoxyquin hapten with chicken egg white albumin is used as a coating agent. An indirect detection enzyme-linked immunoassay method for ethoxyquin is established. The half-inhibition concentration of the method for ethoxyquin is 0.96 ug / mL, the detection limit is 0.6 ug / mL, the linear detection range is 0.72 ug / mL-1.28 ug / mL, and the curve correlation coefficient is 0.996. The method is high in specificity and sensitivity, simple and fast, and has a wide application prospect in the detection of ethoxyquin residues in food. The method is very suitable for realizing rapid qualitative or quantitative detection of ethoxyquin in food by primary site personnel.

The invention provides a hybridoma cell strain secreting a toxoflavin monoclonal antibody. The hybridoma cell strain is obtained by immunogen immune animals prepared by coupling toxoflavin hapten and carrier protein. Wherein the carrier protein for preparing the immunogen comprises keyhole limpet hemocyanin KLH. The hybridoma cell strain is preserved in the Guangdong Microbial Culture Collection Center on March 10, 2026, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.67912. The invention also provides an application of the hybridoma cell strain, specifically, the toxoflavin monoclonal antibody secreted by the hybridoma cell strain is applied to toxoflavin detection. The kit further comprises a coating antigen, the coating antigen is prepared by coupling the toxoflavin hapten and carrier protein, and the carrier protein for preparing the coating antigen comprises bovine serum albumin (BSA). The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to toxoflavin, and can be accurately and reliably used for quantitative detection of toxoflavin in an actual sample.

The invention belongs to the technical field of biological medicines, and particularly relates to a human NSUN2 protein 190th lysine site lactic acid antigen, an antibody, a preparation method and application thereof. Relates to the technical field of lactylated antibody preparation, and the preparation method comprises the following steps: S01, carrying out lactylation on 190th lysine in a polypeptide of an amino acid sequence, and coupling with a carrier protein to obtain an initial antigen, the carrier protein comprising hemocyanin KLH; s02, injecting the obtained antigen into a living body culture sample for immune culture, and collecting whole blood after a preset period to obtain multi-antibody serum; and S03, purifying to obtain an iterative antibody. According to the invention, the expression difference in sensitive and drug-resistant tumor cells and tumor tissues can be detected by directly applying Western Blotting, immunofluorescence and immunohistochemistry, so that a powerful guarantee is provided for the detection of tumor sensitivity.

The application relates to a monoclonal antibody of alpha, beta and gamma amatoxin and a preparation method and application thereof. The monoclonal antibody of alpha amatoxin is prepared from an alpha amatoxin artificial antigen, the monoclonal antibody of beta amatoxin is prepared from a beta amatoxin artificial antigen, and the monoclonal antibody of gamma amatoxin is prepared from a gamma amatoxin artificial antigen; the alpha amatoxin artificial antigen is a conjugate of alpha amatoxin and keyhole limpet hemocyanin, the beta amatoxin artificial antigen is a conjugate of beta amatoxin and bovine serum albumin, and the gamma amatoxin artificial antigen is a conjugate of gamma amatoxin and human serum albumin. The three antibodies have a small cross-reaction rate, can simultaneously detect and distinguish alpha-AMA, beta-AMA and gamma-AMA in a sample, and have high accuracy.

The invention provides a kit for detecting endotoxin and a detection method thereof. The kit comprises: a capture unit: a recombinant crab-like hemocyanin factor C fixed on the surface of a solid phase carrier; the signal probe is composed of a polyclonal anti-lipopolysaccharide antibody coupled with gold nanoparticles and horse radish peroxidase; the reaction buffer solution is a Tris-HCl buffer solution containing calcium ions required by activation of Factor C and a surfactant; the signal substrate solution is a peroxidase chromogenic substrate solution containing 3, 3 ', 5, 5'-tetramethyl benzidine; the stop solution comprises a dilute sulphuric acid solution and an endotoxin standard substance. The rapid, high-sensitivity and high-specificity endotoxin detection kit based on the recombinant factor C and the nano signal amplification technology has the advantages of high specificity, interference resistance, high sensitivity, rapidness, convenience and the like, and is wide in application prospect.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention provides a novel streptococcus group A oligosaccharide antigen and glycobinding protein vaccine, which is characterized in that oligosaccharide antigen containing 30-70% of N-acetyl glucamine multi-rhamnose core skeleton structure is accurately prepared through chemical synthesis, and is efficiently coupled with KLH (the loading capacity is about 215 oligosaccharide / KLH molecules); safe, efficient and broad-spectrum immune protection is realized; safety: the GlcNAc side chain modification design avoids autoimmune risks (such as acute rheumatic fever heat); high efficiency: the animal experiment antibody titer reaches 1: 12, 800; the broad spectrum is as follows: the highly conservative universal GAS oligosaccharide antigen can universally cover various serotypes, and the key synthetic route (Scheme 2, 3, 16 and 20, the yield is 22%-96%) and the loading capacity verification (HPAEC) support the technical feasibility.

The application discloses a New Delhi tomato leaf curl virus coat protein polypeptide, antigen, antibody and application thereof, and belongs to the technical field of organism immunity detection. The application is based on ToLCNDV CP to design a polypeptide sequence, and provides a New Delhi tomato leaf curl virus coat protein polypeptide. The antigen prepared by coupling the polypeptide with keyhole limpet hemocyanin (KLH) can obtain a New Delhi tomato leaf curl virus antibody with high sensitivity and specificity, and can be used for screening the virus, and realizes instant detection and early warning of the ToLCNDV virus. Meanwhile, the application provides a theoretical basis and technical support for establishment of a virus serological diagnosis method and related detection product research and development, and is helpful to development of more accurate, efficient and portable detection products, so as to better serve the virus monitoring, quarantine and prevention and control work.

The invention discloses a yellow-blue discoloration hemocyanin mancozeb compound bactericide and a preparation method thereof, and belongs to the technical field of pesticide preparation, the yellow-blue discoloration hemocyanin mancozeb compound bactericide comprises, by mass, 30%-80% of mancozeb, 0.0001%-1% of hemocyanin, 2%-8% of supramolecular carriers and 4%-65% of auxiliaries, the compound bactericide is yellow, and the compound bactericide becomes blue when making contact with water. According to the invention, mancozeb and hemocyanin are inlaid through the supramolecular carrier, so that efficient synergy of biological protein and a chemical bactericide is realized, same-effect prevention and treatment are realized under the condition that the dosage of mancozeb is reduced, and the pollution to the environment is reduced; the stability of biological protein is improved, and the lasting period of the compound bactericide is prolonged; multi-stage targeted delivery is realized, so that the permeability of the bactericide to crop leaf surfaces is improved, and meanwhile, the drug resistance risk of crops to the bactericide is also reduced; meanwhile, the specific oxygenation discoloration characteristic of the hemocyanin is utilized, so that the drug effect of the sterilization component is visually monitored.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-1A1, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The present application relates to the field of aquatic animal disease-resistant breeding, and discloses a method for multi-index combined detection of the anti-DIV1 virus ability of Macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof, which comprises the following steps: after the Macrobrachium rosenbergii is anesthetized and disinfected, abdominal limb samples are obtained by minimally invasive cutting of abdominal limbs, and the live body is placed in water for recovery; total RNA is extracted and reversely transcribed into a cDNA template; specific primers are used for fluorescence quantitative PCR detection of chitinase 1C gene, C-type lectin domain-containing protein gene, hemocyanin gene, actin gene and an internal reference gene; and the relative expression data are standardized, directionally corrected, weighted and converted to obtain a comprehensive disease resistance index and a determination result. The present application obtains samples by minimally invasive cutting of abdominal limbs, ensures the survival and recovery of the live body, facilitates the in-vivo breeding of excellent individuals, and improves the accuracy and stability of detection and evaluation by combining multi-dimensional target genes for joint detection.

The invention discloses a New Delhi tomato leaf curl virus coat protein polypeptide, an antigen, an antibody and application thereof, belongs to the technical field of organism immunodetection, and provides the New Delhi tomato leaf curl virus coat protein polypeptide based on design of a polypeptide sequence of ToLCNDV CP. The New Delhi tomato leaf curl virus antibody with relatively high sensitivity and specificity can be obtained through an antigen immune experiment body prepared by coupling the new Delhi tomato leaf curl virus antibody with KLH, and can be used for screening the virus, so that instant detection and early warning of the ToLCNDV virus are realized. Meanwhile, a theoretical basis and a technical support are provided for establishment of a virus serological diagnosis method and research and development of related detection products, and development of more accurate, efficient and portable detection products is facilitated, so that monitoring, quarantine and prevention and control work of viruses can be better served.

This invention provides an immunochromatographic time-resolved fluorescence kit for polystyrene micro-nanoplastics and its use. [Solution] The solution comprises a fluorescent test paper strip and a sample reaction flask containing a lyophilized anti-polystyrene monoclonal antibody labeled with quantum dot fluorescent microspheres. The fluorescent test paper strip includes a bottom plate, on which an absorbent pad, a detection pad, and a sample pad are attached from top to bottom on the adhesive surface of the bottom plate, with adjacent pads overlapping and connected at connection points. The detection pad uses a nitrocellulose membrane as its base pad, with a lateral quality control line and a detection line provided on the nitrocellulose membrane from top to bottom. The quality control line is coated with an immunoanti-mouse polyclonal antibody, and the detection line is coated with a polystyrene-hemocyanin conjugate.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-6D6, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.