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520results about How to "Specific" patented technology

Personnel identification method and system combining video identification and UWB positioning technologies

The invention provides a personnel identification method and system combining video identification and UWB (ultra-wideband) positioning technologies. The method comprise the steps of: A, in the simulation filed of a same supervision area, obtaining geographical positions of mobile positioning objects and the first quantity of the geographical positions according to video identification positioning, and obtaining positioning coordinates of positioning terminals and the second quantity of the positioning coordinates according to wireless positioning; B, comparing the first quantity and the second quantity at a same time, prompting abnormity and entering C if the first quantity is not equal to the second quantity, and prompting normality if the first quantity is equal to the second quantity; and C, obtaining abnormal geographical positions according to the matching between the positioning coordinates and the geographical positions. The method and system can efficiently monitor specific objects and specific occasions such as a campus, analyze and determine in real time position monitoring and video image monitoring information, and timely provide alarm response service, thereby enhancing campus safety service.
Owner:上海真灼科技股份有限公司

Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR

ActiveUS20130177915A1Strong specificityRetain specificityMicrobiological testing/measurementDna polymerasenGenetics
There is provided a method for detecting a target RNA molecule in a sample. The method comprises reverse transcribing the target RNA contained in the sample using an RT oligonucleotide, the RT oligonucleotide comprising a stem-loop portion containing one or more nucleotides modified or modifiable to block DNA polymerase extension and a target annealing portion that is complementary to a downstream portion of the target RNA, the target annealing portion located 3′ to the stem-loop portion, to produce a reverse transcription product that comprises the RT oligonucleotide and a 3′ extended region; amplifying the reverse transcription product using (i) a first amplification primer that anneals to a downstream portion of the 3′ extended region of the reverse transcription product and (ii) a second amplification primer that anneals to an interface portion of a DNA strand complementary to the reverse transcription product, the interface portion comprising a region that is complementary to a 3′ portion of the RT oligonucleotide and a 5′ portion of the 3′ extended region in the reverse transcription product, to produce an amplification product; and detecting the amplification product; wherein the stem-loop portion adopts a stem-loop structure under conditions used for said reverse transcribing but does not adopt the stem-loop structure under conditions used for said amplifying and wherein when the stem-loop portion contains one or more nucleotides that are modifiable to block DNA polymerase extension, the method further comprises modifying the modifiable nucleotide prior to said amplifying.
Owner:NAT UNIV OF SINGAPORE

FPGA (Field Programmable Gate Array)-based pulse wave signal generator and method

The invention discloses an FPGA (Field Programmable Gate Array)-based pulse wave signal generator comprising an LCD (Liquid Crystal Display), an oscilloscope, an FPGA, a touch screen, a power supply, an AD (Analog-to-Digital) conversion device and a DA (Digital-to-Analog) conversion device, wherein the input end of the DA conversion device is connected with the FPGA, and the output end of the DA conversion device is connected with the oscilloscope; the input end of the AD conversion device is connected with the FPGA, and the output end of the AD conversion device is connected with the touch screen; the LCD is connected with the FPGA; and the power supply is connected with the LCD. A waveform of a pulse wave is generated in two ways in a method provided by the invention, wherein one way is a Gaussian function and Cosine function combination way, the pulse wave is subjected to segmentation fitting to obtain the final complete waveform, the storage space wasted by the whole waveform is omitted, and the Gaussian function and Cosine function combination way has specificity; and the other way is as follows: an elastic cavity model is adopted for simulating the condition of a vessel to form two elastic cavities of the vessel, and waveforms of pulse waves under four conditions of low resistance, middle resistance, high resistance and ultrahigh resistance can be generated through changing parameters. The both ways can be set by a user on the premise that signal data is unknown, so that an accurate waveform of the pulse wave is obtained and output.
Owner:NORTHEASTERN UNIV

Surface modified carbon nano point, preparation thereof, application of surface modified carbon nano point used as fluorescence probe for detecting Cu2+ and glutathione

The invention relates to a surface modified carbon nano point, preparation thereof, application of the surface modified carbon nano point used as a fluorescence probe for detecting Cu2+ and glutathione. The invention relates to a detection method of the Cu2+ and the glutathione, and aims to solve the technical problems that an existing fluorescence probe for detecting the Cu2+ is easy to interfere by other metal ions, high in preparation cost and complex in preparation step. The triethylene tetramine-modified carbon nano point is used as the fluorescence probe for detecting the Cu2+ and the glutathione; the preparation method of the fluorescence probe comprises the steps of microwave heating citric acid and urea in a water solution, adding the acquired carbon nano point into an ethanol solution containing triethylene tetramine for reflowing, and preparing the fluorescence probe. The preparation method is simple, the reaction conditions are moderate, and the cost is low. A detection method comprises the steps of fluorescence quenching TCDs and forming a TCDs-Cu2+ compound through interaction of the fluorescence probe and the Cu2+, further interacting the TCDs-Cu2+ compound and the glutathione, recovering fluorescence of the TCDs, and detecting the Cu2+ and the glutathione. The probe can be used for detecting copper ions and the glutathione in the water solution and a life entity.
Owner:QIQIHAR UNIVERSITY
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