12results about How to "Specific" patented technology

The invention discloses an E3 ubiquitin enzyme mutant as well as modification and a protein targeted degradation system and application thereof, and particularly relates to a series of targeted protein degradation enhanced E3 ubiquitin enzymes (Trim21-N) and a modification method thereof, the E3 ubiquitin enzymes (Trim21-N) are obtained by modifying a wild type Trim21 (wtTrim21) protein structural domain, and the E3 ubiquitin enzymes comprise Trim21-a, Trim21-b, Trim21-c, Trim21-d, Trim21-e and Trim21-f. The efficiency of degrading the target protein through the ubiquitin proteasome is remarkably improved, and the method has the characteristics of strong endogenous and exogenous universality, high specificity, ubiquitin proteasome dependence, low cytotoxicity, strong cell compatibility and the like on the degradation of the target protein. The invention provides E3 ubiquitin enzyme (Trim21-N) based on a targeted protein degradation enhancement version and an exogenous fluorescence report degradation system of the E3 ubiquitin enzyme (Trim21-N), preferably Trim21-e with the optimal degradation effect, and the E3 ubiquitin enzyme (Trim21-N) is named as delta Trim21. The invention further provides an endogenous protein degradation system based on the delta Trim21. The method has the characteristics of high specificity, rapidness and small module, so that the method can be used for researching various biological and disease mechanisms, including the fields of cell targeted therapy, cell mechanism exploration, genetic engineering and the like.

The invention discloses a monoclonal antibody composition for resisting neurofilament light chain protein and application, the monoclonal antibody composition comprises NfL-Clone 1 and NfL-Clone 2, the amino acid sequence of a heavy chain variable region of the NfL-Clone 1 is as shown in SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the NfL-Clone 1 is as shown in SEQ ID NO.2; the amino acid sequence of a heavy chain variable region of the NfL-Clone 2 is as shown in SEQ ID NO.3, and the amino acid sequence of a light chain variable region of the NfL-Clone 2 is as shown in SEQ ID NO.4. The antibody combination has the characteristics of specificity and high affinity, the NfL can be used as a brain injury biomarker, and meanwhile, the invention also provides a diagnostic detection kit of the antibody for early diagnosis of brain injury of a patient, so that the specificity and sensitivity of a diagnostic reagent are improved.

This invention relates to the field of biomedical detection technology, providing a reagent, kit, and detection method for detecting *Vibrio cholerae* serogroup O139 using loop-mediated isothermal amplification (LAMP). This invention targets the synergistic regulation of the tcpA and hlyA genes in *Vibrio cholerae* serogroup O139, designing LAMP detection primers and establishing a LAMP detection method based on isothermal amplification. These LAMP detection primers or loop primers show promising applications not only against *Vibrio cholerae* serogroup O139 but also against other nosocomial infection bacteria. The LAMP primers for detecting *Vibrio cholerae* serogroup O139 can be used to detect *Vibrio cholerae* serogroup O139 in clinical patients or for outpatient screening. The kit of this invention can effectively and specifically detect single-reaction, single-copy, or higher copies of *Vibrio cholerae* serogroup O139. Compared to the "gold standard" real-time quantitative PCR, the kit of this invention offers higher accuracy and sensitivity, completing the detection within 30 minutes.

The utility model belongs to the field of motorcycle accessory production and processing equipment, and provides a motorcycle transmission fixing plate processing mechanical arm which comprises a multi-degree-of-freedom mechanical arm, a tail end joint and a polishing device, the polishing device comprises a mounting base, a connecting base, a polishing motor, a polishing base and a working shaft, a bowl-shaped opening is formed in the end of the working shaft, and the polishing motor is connected with the mounting base. The bottom face of the bowl-shaped opening is provided with an inserting groove and a sand plate, the bottom face of the sand plate is used for being matched with a generatrix of a transmission fixing plate, the top face of the bowl-shaped opening is provided with a plurality of semi-annular holes, the polishing base is provided with air pressure connecting nozzles obliquely communicated with the semi-annular holes, and the air pressure connecting nozzles are used for being connected with an air source. The polishing device is adjusted through the multi-degree-of-freedom mechanical arm, polishing operation is completed in a covering mode, design is reasonable, specificity is achieved, unified product quality can be guaranteed easily, polished chippings can leave a sand plate in time in an air blowing mode, the quality of next-time polishing operation can be guaranteed easily, and the polishing device is suitable for large-scale popularization.

The present invention provides a probe and a detection method for specifically detecting type I collagen, wherein the probe comprises a polypeptide and a lanthanide ion, wherein the polypeptide is an X-targeted polypeptide, wherein X is 5-carboxyl-1,10-phenanthroline, and its name is Phen, and 5-carboxyl-1,10-phenanthroline is combined with the lanthanide ion through electrostatic interaction. The probe can specifically detect type I collagen, and the detection method is simple and efficient. The probe has targeting, a stable fluorescence signal and lifespan, can avoid the problems of photobleaching and unstable emission peak, and has great potential in the accurate diagnosis of collagen-related diseases.

The invention relates to an ultrasonic transducer excitation system and method, in particular to an ultrasonic transducer excitation system and method for generating adjustable Gaussian signals. The invention aims to solve the problems that an ultrasonic transducer corresponding to a Gaussian signal serving as an ultrasonic excitation signal is complicated in circuit structure compared with transducers of other excitation signals, and errors cannot be effectively controlled. The system comprises a digital-to-analog conversion circuit, a power supply circuit, an operational amplifier circuit and an FPGA internal excitation system, the FPGA internal excitation system receives a signal sent by an upper computer, a signal output end of the FPGA internal excitation system is connected with a signal receiving end of the digital-to-analog conversion circuit, a signal output end of the digital-to-analog conversion circuit is connected with a signal receiving end of the operational amplifier circuit, the operational amplifier circuit emits an excitation signal, and the signal receiving end of the operational amplifier circuit receives the excitation signal. The power supply circuit provides power for the FPGA internal excitation system, the digital-to-analog conversion circuit and the operational amplification circuit. The invention belongs to the technical field of digital signal processing.

The invention discloses a method for extracting pine wood nematode DNA in a wood sample, and pine wood nematode diseases are pine tree diseases caused by pine wood nematode, have high destructiveness and severely harm forest resources. Therefore, the detection and prevention and control of the pine wood nematode disease are of great significance. However, an existing pine wood nematode disease detection method still has various limitations, and particularly, the process of extracting pine wood nematode DNA from a wood sample is complex in operation, long in time and high in cost. According to the scheme, extraction conditions are screened, and it is determined that an ultrasonic cell crusher is used for carrying out bursaphelenchus xylophilus cell crushing on a wood sample; by sampling different positions of the cross section of the tree, the distribution of the pine wood nematodes in the inner ring of the wood is determined; through screening by a sampling method, tree sampling by using the growth cone is determined. Finally, the method for rapidly extracting the DNA of the pine wood nematode in the wood sample is established, and the method can make the diagnosis of the pine wood nematode disease faster, simpler and more economical, and lays a foundation for the prevention and treatment of the pine wood nematode disease.

The invention discloses an insect delta7-sterol C5 desaturase (delta7-steril C5-class-like, SC5DL), and an amino acid sequence of the insect delta7-sterol C5 desaturase is as shown in SEQ ID NO. 2. The insect delta7-sterol C5 desaturase has an amino acid sequence as shown in SEQ ID NO. The invention also discloses a specific fragment of the SC5DL gene, the specific fragment is used for synthesizing dsRNA, the expression level of the gene can be specifically inhibited by utilizing the dsRNA, the cholesterol synthesis of bemisia tabaci can be inhibited, and finally the reproductive capacity of the bemisia tabaci can be obviously reduced. A new target is provided for a bemisia tabaci prevention and control technology based on RNA interference, and a green and sustainable scheme is provided for solving the contradiction between chemical pesticide and field bemisia tabaci resistance.

The present invention proposes a purification preparation method and application of an anti-diphtheria toxin mutant antibody and CRM197. On the basis of developing and preparing CRM197 antigen, the present invention further immunizes mice with diphtheria toxin mutants, fuses mouse spleen cells with mouse myeloma cells, screens hybridoma cell lines that produce specific monoclonal antibodies against diphtheria toxin mutants, and obtains anti-diphtheria toxin mutant antibody sequences after sequencing. After the hybridoma cells are cultured in mice, anti-CRM197 antibodies can be prepared in vitro by chromatography purification and other methods. The antibody has a high affinity with CRM197, can be used for the purification preparation of CRM197 protein, can also be used for the purification preparation of coupled vaccines with CRM197 as a carrier, and further can be used for enzyme-linked immunosorbent assay to detect the content of CRM197 in coupled vaccines with CRM197 as a carrier.

The invention relates to the field of molecular biology, in particular to a primer group, an MYH3 gene promoter region DNA methylation level analysis method and application. The primer group comprises a first primer pair and a second primer pair, the upstream sequence of the first primer pair is as shown in SEQ ID NO. 1; the downstream sequence of the first primer pair is as shown in SEQ ID NO. 2; the upstream sequence of the second primer pair is as shown in SEQ ID NO.3; and the downstream sequence of the second primer pair is as shown in SEQ ID NO.4. The method for analyzing the methylation level of the DNA in the MYH3 gene promoter region relates to PCR reaction conditions for obtaining qualified target DNA fragments for MSP sequencing, the primer group and the PCR reaction conditions are combined to have the advantage of high amplification efficiency, the qualified DNA fragments are obtained through amplification, the DNA fragments are provided for analyzing the methylation level of the DNA in the MYH3 gene promoter region, and the methylation level analysis efficiency and accuracy are improved.

The invention discloses application of bupleurum polysaccharide in preparation of a medicine for resisting liver transplantation rejection, the weight-average molecular weight of the bupleurum polysaccharide is 10-600kDa, and the bupleurum polysaccharide comprises monosaccharides such as mannose, galacturonic acid, glucose, galactose and arabinose. The polysaccharide disclosed by the invention has the effect of resisting liver transplantation rejection, has the potential of inducing immune tolerance of an organism, and is expected to realize final evacuation of an immunosuppressor, so that adverse reaction caused by long-term administration is avoided. The polysaccharide mainly acts on liver macrophages, has tissue specificity, and is non-toxic and good in safety, so that the polysaccharide is expected to be developed into a treatment or adjuvant treatment medicine for resisting liver transplantation immunological rejection with good specificity and low adverse reaction.

The application discloses a preparation method and use method of an electrochemical molecular imprinting sensor for detecting DBP. A PEDOT-PG nanocomposite is prepared through a liquid-liquid interface polymerization method, then the prepared PEDOT-PG is functionalized by using polylysine, so that the PEDOT / PG has good dispersity and stability, and good adhesion on the surface of the sensor. In the presence of DBP, a molecular imprinting film is prepared through a monomer electropolymerization method, so that the recognition response speed of the target molecule is improved, the molecular imprinting sensor has the advantages of structural stability, high mechanical strength, easy acquisition and low cost, and has the advantages of specificity for recognizing the target molecule.