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This invention relates to the field of biomedical detection technology, providing a reagent, kit, and detection method for detecting *Vibrio cholerae* serogroup O139 using loop-mediated isothermal amplification (LAMP). This invention targets the synergistic regulation of the tcpA and hlyA genes in *Vibrio cholerae* serogroup O139, designing LAMP detection primers and establishing a LAMP detection method based on isothermal amplification. These LAMP detection primers or loop primers show promising applications not only against *Vibrio cholerae* serogroup O139 but also against other nosocomial infection bacteria. The LAMP primers for detecting *Vibrio cholerae* serogroup O139 can be used to detect *Vibrio cholerae* serogroup O139 in clinical patients or for outpatient screening. The kit of this invention can effectively and specifically detect single-reaction, single-copy, or higher copies of *Vibrio cholerae* serogroup O139. Compared to the "gold standard" real-time quantitative PCR, the kit of this invention offers higher accuracy and sensitivity, completing the detection within 30 minutes.

The application discloses a preparation method and use method of an electrochemical molecular imprinting sensor for detecting DBP. A PEDOT-PG nanocomposite is prepared through a liquid-liquid interface polymerization method, then the prepared PEDOT-PG is functionalized by using polylysine, so that the PEDOT / PG has good dispersity and stability, and good adhesion on the surface of the sensor. In the presence of DBP, a molecular imprinting film is prepared through a monomer electropolymerization method, so that the recognition response speed of the target molecule is improved, the molecular imprinting sensor has the advantages of structural stability, high mechanical strength, easy acquisition and low cost, and has the advantages of specificity for recognizing the target molecule.