Surface modified carbon nano point, preparation thereof, application of surface modified carbon nano point used as fluorescence probe for detecting Cu2+ and glutathione

A technology of carbon nanodots and surface modification, applied in the field of nano-fluorescence sensors, can solve the problems of easy interference of fluorescent probes by other metal ions, high preparation cost, complicated preparation steps, etc.

Inactive Publication Date: 2017-06-13
QIQIHAR UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] The present invention is to solve the existing detection Cu 2+ Fluorescent probes are susceptible to interference from other metal ions, high preparation costs, and complicated preparation steps. However, surface-modified carbon nanodots and their preparation and use as fluorescent probes to detect Cu 2+ and the application of glutathione to realize the highly selective fluorescence detection of Cu 2+ and glutathione

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  • Surface modified carbon nano point, preparation thereof, application of surface modified carbon nano point used as fluorescence probe for detecting Cu2+ and glutathione
  • Surface modified carbon nano point, preparation thereof, application of surface modified carbon nano point used as fluorescence probe for detecting Cu2+ and glutathione
  • Surface modified carbon nano point, preparation thereof, application of surface modified carbon nano point used as fluorescence probe for detecting Cu2+ and glutathione

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specific Embodiment approach 1

[0038] Embodiment 1: The surface-modified carbon nano-dots in this embodiment are triethylenetetramine-modified carbon nano-dots with a particle size of 3-5 nm.

specific Embodiment approach 2

[0039] Specific embodiment two: the preparation method of the surface-modified carbon nano-dot described in specific embodiment one is carried out according to the following steps:

[0040] 1. The mass ratio of carbon nano-dots and triethylene tetramine is 1: (0.1-10) adding carbon nano-dots and triethylene tetramine to ethanol and mixing uniformly to obtain a mixture;

[0041]2. Heating the mixture to reflux for 5-48 hours to obtain a crude product;

[0042] 3. Rotate the crude product to remove ethanol, and then wash the crude product with benzene for 1 to 5 times. After centrifugation, dissolve the solid phase with ultrapure water to obtain a solution, and then filter the solution; vacuum-dry the filtrate to obtain the surface Modified carbon nanodots (TCDs).

specific Embodiment approach 3

[0043] Specific embodiment three: the difference between this embodiment and specific embodiment two is that the carbon nano-dots (CDs) described in step one are mixed by urea and citric acid in the ratio of mass ratio 1: (1~6), and then passed A carbon-rich nanoparticle prepared by microwave heating; the others are the same as in the second embodiment.

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Abstract

The invention relates to a surface modified carbon nano point, preparation thereof, application of the surface modified carbon nano point used as a fluorescence probe for detecting Cu2+ and glutathione. The invention relates to a detection method of the Cu2+ and the glutathione, and aims to solve the technical problems that an existing fluorescence probe for detecting the Cu2+ is easy to interfere by other metal ions, high in preparation cost and complex in preparation step. The triethylene tetramine-modified carbon nano point is used as the fluorescence probe for detecting the Cu2+ and the glutathione; the preparation method of the fluorescence probe comprises the steps of microwave heating citric acid and urea in a water solution, adding the acquired carbon nano point into an ethanol solution containing triethylene tetramine for reflowing, and preparing the fluorescence probe. The preparation method is simple, the reaction conditions are moderate, and the cost is low. A detection method comprises the steps of fluorescence quenching TCDs and forming a TCDs-Cu2+ compound through interaction of the fluorescence probe and the Cu2+, further interacting the TCDs-Cu2+ compound and the glutathione, recovering fluorescence of the TCDs, and detecting the Cu2+ and the glutathione. The probe can be used for detecting copper ions and the glutathione in the water solution and a life entity.

Description

technical field [0001] The invention belongs to the field of nano-fluorescent sensors, in particular to the detection of Cu by fluorescent probes 2+ and glutathione methods. Background technique [0002] Copper is one of the essential trace elements for life and plays an important role in the physiological process of the human body. However, when the concentration of copper ions in the human body exceeds a certain concentration, it can also cause poisoning and cause serious irreversible nerve damage. In addition, tripeptides rich in sulfhydryl groups, such as glutathione (GSH), exist in almost every cell of the human body, which can help the human body maintain normal immune function. Detection of Cu 2+ And glutathione and other important biologically active substances in living bodies, and the study of their interaction has special significance for biology, chemistry, and the environment. There are currently fluorescent methods for the detection of Cu 2+ For example, M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C09K11/02G01N21/64
CPCC09K11/025C09K11/65G01N21/6428G01N21/6486G01N2021/6432
Inventor 郭祥峰杨瑞刘富强王鑫鑫付立凤何刘明慧贾丽华
Owner QIQIHAR UNIVERSITY
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