420 results about "Red fluorescence" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention discloses an MOF-CQD enzyme-sensitive FRET hydrogel diagnosis and treatment probe as well as a preparation method and application thereof, and belongs to the technical field of biomedical detection and treatment. The probe takes a carboxyl carbon quantum dot (CQD) as a donor and a metal organic framework (MOF) as an acceptor, and the donor and the acceptor are connected through a replaceable enzyme response polypeptide bridge; when the fluorescence is complete, FRET is generated to quench the CQD fluorescence, the FRET is interrupted after the target enzyme cuts off the peptide bridge, and the blue / red fluorescence ratio is changed to realize nanomole-level quantitative detection and has self-correction capability. The probe is packaged in a four-arm PEG transparent hydrogel which does not need a photoinitiator and can be quickly self-crosslinked, and can be prepared into a film, a tooth socket and the like for POCT (point-of-care testing) of parts such as an oral cavity / skin and the like. After detection, the MOF is activated by illumination of 630-660 nm to generate active singlet oxygen, so that local antibacterial or anti-tumor treatment is realized, and a diagnosis and treatment integrated scheme is constructed.

The invention is about a method of marking ink which is only used to defend fake. The composition of it is: lipidol or lipid, surface active agent, integration agent, water, buffering agent. Their mass percents are: 1,2-hexylene glycol: 5%-15%, butanol: 1-5%, DH-1: 5-10%, EDTA: 0.1-0.5%, MS-1: 0.1-1%, water: the rest. The process is: a. dissolving the colorant in water, b. put the correct percent of 1,2-hexylene glycol and butanol into the solvent, then dropping the MS-1 and EDTA solution when mixing the solvent for 30-50 min, c. put the rest precent of water into the printing ink box. The picture and word would be shown red fluorescence in the UV but blank in the usual light.

A phosphor composition includes a red phosphor material having a red decay rate and a green phosphor material having a green decay rate. The red phosphor material includes a Mn4+ doped phosphor of Formula I and a Eu3+ doped uranium phosphor, and a difference between the red decay rate and the green decay rate is no more than 7 ms, AxMFy:Mn4+ (I), wherein A is Li, Na, K, Rb, Cs, or a combination thereof; M is Si, Ge, Sn, Ti, Zr, Al, Ga, In, Sc, Hf, Y, La, Nb, Ta, Bi, Gd, or a combination thereof; x is an absolute value of a charge of the MFy ion; and y is 5, 6 or 7. A device is also provided.

The invention is suitable for the technical field of inorganic luminescent materials, and provides a fluorescent powder material based on a double-perovskite structure and a preparation method and application thereof. The general chemical formula of the fluorescent powder is Ca2AlNbO6: xEu < 3 + >, wherein x is a molar fraction of 0.06 to 0.30; the excitation spectrum coverage range of the fluorescent powder under the condition of monitoring the emission wavelength of 615 nm is 260-500 nm, and the emission main peak is located at 615 nm; when x is equal to 0.18, the quantum efficiency under the excitation of 396nm ultraviolet light is greater than or equal to 91%, and the red light emission ratio is greater than or equal to 85%. According to the invention, Ca2AlNbO6 double perovskite is taken as a substrate, and a red fluorescent powder material with wide-spectrum excitation and high quantum efficiency is designed through Eu < 3 + > high-concentration doping and local symmetry regulation and control design engineering, so that the characteristics of high quantum efficiency, efficient red light emission, wide-spectrum excitation and excellent concentration quenching resistance are realized.

The invention relates to a double-probe detection reagent for detecting salmonella typhimurium and a preparation method of the double-probe detection reagent, and belongs to the technical field of food safety. The double-probe detection reagent is formed by respectively packaging a magnetic separation probe reagent solution and a fluorescent nano-enzyme probe reagent solution; the magnetic separation probe reagent liquid is used for specifically binding salmonella typhimurium and carrying out separation and enrichment treatment on a detection sample; the red fluorescent nano-enzyme probe reagent solution is used for specifically combining the salmonella typhimurium subjected to separation and enrichment treatment and generating a salmonella typhimurium concentration dependent colorimetric signal and a fluorescent signal. The double-probe detection reagent disclosed by the invention is short in detection time and high in detection sensitivity when being used for detecting salmonella typhimurium, and has relatively good specificity on common type strains. According to the invention, the specific surface area is high, the peroxidase-like activity is excellent, and the high-sensitivity capture efficiency is enhanced; according to the invention, mutual verification is carried out between the dual-mode signals, an internal proofreading effect is achieved, and the detection result is accurate.

The invention relates to the technical field of organic supramolecular chemical materials, in particular to an octyloxy-modified imine gelator, bi-component gel of the octyloxy-modified imine gelator and a preparation method and application of the octyloxy-modified imine gelator. The gelator is a symmetric o-octyloxy modified p-phenylenedialdehyde-maleonitrile imine gelator, and has an obvious aggregation-induced emission property; the gelator and the cholesteryl half-ester gel can form double-component organic gel without fluorescence emission in an organic solvent; the fluorescence-emission-free double-component gel formed in tert-butyl alcohol is red fluorescence-emission gel when meeting water, and is converted into blue fluorescence-emission gel when meeting NaClO, so that water and NaClO can be rapidly and intuitively detected; the gelator has fluorescence enhancement and blue shift after encountering NaClO in a solution state, has fluorescence'off-on 'detection on NaClO, has fluorescence emission intensity reduced after Hg < 2 + > is added in the solution state, and has fluorescence'on-off' detection performance on Hg < 2 + >.

The invention relates to the technical field of organic material detection, in particular to an imine molecular sensor with a five-detection function, two-component gel as well as a preparation method and application of the imine molecular sensor. The imine molecular sensor has three signal responses of fluorescence on-off, ultraviolet absorption and color change to Fe < 3 + >, Co < 2 + > and Ni < 2 + > in a solution state, and has ultraviolet absorption and fluorescence on-off dual-detection performance to acid and alkali changes; the imine molecular sensor and cholesteryl half ester can form two-component gel through a heating-low-temperature ultrasonic cooling method; after Fe < 3 + >, Co < 2 + >, Ni < 2 + >, NaOH and HCl are respectively added, the orange of the gel becomes milky white, reddish brown, transparent red, milky white and milky white under sunlight, and the fluorescence of the orange of the gel disappears under an ultraviolet lamp, so that the rapid and sensitive detection of Fe < 3 + >, Co < 2 + >, Ni < 2 + >, acid and alkali under two states of the imine molecular solution and the gel is realized. The imine molecular sensor provided by the invention has the advantages of mild synthesis conditions, simple preparation and the like, and creates favorable conditions for popularization and application of the imine molecular sensor.

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention discloses a watermelon transgenic screening expression cassette and application thereof, and belongs to the technical field of biology. According to the invention, an expression cassette of a TagRFP gene driven by a d35s promoter is constructed on a pCambia1300 vector, and a recombinant vector is obtained. A recombinant vector is introduced into watermelon cells by utilizing an agrobacterium-mediated genetic transformation technology, and a red fluorescent protein TagRFP gene is integrated into a watermelon genome, so that transgenic watermelons can be screened and identified by taking red fluorescence as an identifier in the whole growth period of watermelon genetic transformation. Compared with a traditional screening method, the screening method has the advantages that the screening process is greatly simplified, and the transgenic watermelon and the non-transgenic watermelon are clearly distinguished, so that the genetic transformation efficiency of the watermelon is remarkably improved.

The invention relates to a red fluorescent material excited by blue light, an LED device and fluorescent ceramic. The chemical formula of the red fluorescent material is La (2-x) MgNbO6.5: xEu < 3 + >, wherein x is more than or equal to 0.01 and less than or equal to 0.1. The preparation method comprises the following steps: weighing lanthanum oxide, magnesium oxide, niobium oxide and europium oxide raw materials according to a stoichiometric ratio, grinding and uniformly mixing; sintering the uniformly mixed powder in a muffle furnace at high temperature, and cooling to room temperature to obtain the red fluorescent material. Compared with the prior art, the novel oxide-based red fluorescent powder prepared by the invention can emit positive red light of which the central wavelength is 616nm under the excitation of 465nm blue light, and can be used for white light LED (Light Emitting Diode) luminescent devices and fluorescent ceramics.

The invention discloses a diamond low-frequency current measurement method with nonlinear response to magnetic field intensity, which is characterized in that two coils are wound on a magnetic flux collector with a communicated structure, a high-frequency (800Hz) high-voltage (7V) driving current is injected into one of the coils, a magnetic field generated by the coil is amplified through the magnetic flux collector and guided to an area where an NV color center magnetometer is located, and the NV color center magnetometer is located; and the signal is driven to enter a nonlinear response interval in the demodulated ODMR spectrum. A weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal is injected into the other coil, amplitude modulation is performed on the weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal by using the same frequency as the driving current, and the weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal is amplified by the magnetic flux collector and guided to the NV The microwave frequency is locked at the maximum slope point of the ODMR frequency spectrum through CW-ODMR phase-locked amplification, so that the relationship between the change of the red fluorescence signal intensity and the size of the external magnetic field is obtained, and the signal intensity of the low-frequency current magnetic field to be measured is measured according to the relationship.

The invention discloses a fixing reagent and method for double-color fluorescence detection of an anti-NMDAR antibody and application, the fixing reagent comprises paraformaldehyde and a mixture of methanol and acetone, and the mixture comprises 10-25% by volume of methanol, 75-90% by volume of acetone, 10-25% by volume of paraformaldehyde, 10-25% by volume of paraformaldehyde and 10-25% by volume of paraformaldehyde. The fixing effect is better by adopting paraformaldehyde with the mass-volume ratio concentration of 4% and a mixture of methanol with the volume fraction of 15% and acetone with the volume fraction of 85% in two steps. By adopting the fixing liquid and the fixing method, the antigenicity of NMDAR cannot be damaged, obvious green fluorescence cells can be seen in a positive sample, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed by using a fluorescence microscope.

The invention relates to a fluorescence in-situ hybridization image result analysis method and system based on deep learning, and a medium. The method comprises the following steps: constructing an SEAM-Unet + + image segmentation model; a YOLO-SEM small target detection algorithm model is constructed; obtaining a cell precise segmentation map of a channel B; obtaining a red fluorescence probe distribution map; obtaining a green fluorescent probe distribution map; and combining the obtained accurate cell segmentation map, the red fluorescence distribution map and the green fluorescence distribution map into one map to obtain a relational graph of cell and probe distribution. According to the method, complex image scenes can be effectively processed, the analysis of various medical images is supported, the medical diagnosis efficiency is improved, personal errors are reduced, and the cell analysis and pathological detection capabilities in scientific research and clinical application are enhanced.

The invention discloses a red fluorescent silicon nanodot as well as a preparation method and application thereof. The method comprises the following steps: S1, weighing sulforhodamine 101, adding ultrapure water for dissolving, then adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, uniformly stirring, transferring the obtained mixture into a reaction kettle, and heating for reaction; and S2, after the reaction is finished, cooling to room temperature, purifying the obtained product by using a silica gel chromatographic column, eluting by using an eluent, collecting the eluent, and finally, freeze-drying to obtain the red fluorescent silicon nanodots. Compared with common blue-green fluorescence, red fluorescence emission has deeper tissue penetrability and background fluorescence interference resistance; the silicon nanodot provided by the invention not only has excellent light stability and high quantum yield, but also has characteristics similar to lipophilic cations, and can specifically target mitochondria in living cells; in addition, the silicon nanodots also show excellent imaging ability in zebrafish embryos and juvenile fish bodies.

The present invention discloses a ratiometric fluorescent smart label for visually detecting the freshness of meat, and its preparation method and application. The smart label preparation method is specifically as follows: first, a ratiometric fluorescent indicator of gold-copper bimetallic nanoclusters (indicator) with red fluorescence and dual-ligand gold nanoclusters (internal standard) with green fluorescence is prepared; then, by a solution doping method, the ratiometric fluorescent indicator is added to a polyvinyl alcohol / polyethylene glycol solution to prepare a ratiometric fluorescent smart label; finally, a fluorescent colorimetric card is made based on the ratiometric fluorescent smart label, and the smart label is applied to the prediction of meat freshness grade and total volatile basic nitrogen content in combination with a smart phone. The ratiometric fluorescent smart label prepared by the present invention has higher accuracy and sensitivity than a colorimetric sensing indicator film; and the smart label is safe, biocompatible, biodegradable, and has strong mechanical properties, and has the function of real-time monitoring of the freshness of fresh meat and aquatic products.

The invention belongs to the technical field of laser diode illumination and display, and particularly relates to a laser white light device for laser display and illumination and a preparation method thereof. Comprising a laser emission module and a fluorescence conversion module. The fluorescence conversion module comprises a pixelated transparent substrate, a green fluorescent powder unit and a red fluorescent powder unit; groove array structures which are arranged in a staggered mode are distributed on the upper side and the lower side of the pixelated transparent substrate respectively. The green fluorescent powder unit is arranged in the lower side groove array structure, and the red fluorescent powder unit is arranged in the upper side groove array structure; the laser emitting module emits laser from the lower portion of the pixelated transparent substrate to the upper portion, and the green fluorescent powder unit and the red fluorescent powder unit are excited to emit green light and red light respectively and then are fused to form white light. According to the invention, the heat dissipation performance of the fluorescence conversion module is improved, the reabsorption between red and green fluorescent powder is weakened, the luminous efficiency is improved, and a white light LD device with high brightness and high color rendering can be realized.

The invention relates to a dental plaque detection method and system based on ultraviolet image analysis. The method comprises the following steps: acquiring an RGB image of dental plaque; converting the RGB image into an HSV image; performing tooth region identification, single tooth segmentation and single tooth contour cutting on the HSV image in sequence to obtain each single tooth ROI pixel and a total tooth ROI pixel; extracting a red fluorescence characteristic region from the HSV image, and calculating to obtain a red fluorescence region pixel and a red fluorescence average brightness value; the dental plaque severity score is calculated according to the total tooth ROI pixel, the red fluorescence region pixel and the red fluorescence average brightness value, the severity of the dental plaque is quantified, a user can know the tooth health state more clearly, and then oral health is maintained. The change of the score value is beneficial to motivating the user to continuously monitor the dental plaque condition, so that the user can conveniently manage oral hygiene and health more finely, and the user experience is improved.

The present invention relates to a dual-probe detection reagent and a preparation method for detecting Salmonella typhimurium, and belongs to the field of food safety technology. The dual-probe detection reagent is composed of a magnetic separation probe reagent solution and a fluorescent nanozyme probe reagent solution, which are packaged separately; the magnetic separation probe reagent solution is used to specifically bind to Salmonella typhimurium and separate and enrich the test sample; the red fluorescent nanozyme probe reagent solution is used to specifically bind to the Salmonella typhimurium after separation and enrichment, and generate a Salmonella typhimurium concentration-dependent colorimetric signal and a fluorescent signal. The dual-probe detection reagent of the present invention has a short detection time and high detection sensitivity for the detection of Salmonella typhimurium, and has good specificity for common model strains. The present invention has a high specific surface area and excellent peroxidase-like activity, which enhances the high-sensitivity capture efficiency; the dual-modal signals of the present invention verify each other, play an internal proofreading role, and the detection results are accurate.

The invention provides a red fluorescent carbon nanodot as well as a preparation method and application thereof. The preparation method of the red fluorescent carbon nanodot provided by the invention comprises the following steps: weighing 0.1-2.8 g of 1, 8-diaminonaphthalene solid, dissolving the 1, 8-diaminonaphthalene solid in 20mL of phosphoric acid, and magnetically stirring to dissolve the 1, 8-diaminonaphthalene solid; transferring the solution into a hydrothermal kettle, carrying out hydrothermal reaction for 6-26 hours under the condition of 120-300 DEG C, and cooling the reaction kettle to room temperature in a natural environment after heating is finished; purifying by using a column chromatography, and taking absolute ethyl alcohol as a washing solvent to obtain a pure carbon dot solution; and finally, evaporating the ethanol solvent by adopting a rotary evaporator, and drying to obtain pure carbon nanodot solid powder. The carbon dot solution can emit bright red fluorescence under the excitation of xenon lamp light with the wavelength of 380-550 nm. The method has a wide application prospect in the fields of time-resolved imaging, advanced information encryption, anti-counterfeiting and the like.

The application provides a manganese-activated antimony acid-based fluorescent powder and a preparation method and application thereof, and relates to the technical field of luminescent materials. 1‑ x Y x )3Li5Sb 2‑y O 12 :yMn, and 0 < x <= 0.3, 0.001 <= y <= 0.01. 3+ The Y 3+ can improve the luminous efficiency and thermal stability of the fluorescent powder, and the fluorescent powder can emit red fluorescence with a wave band of 650nm-770nm under near-ultraviolet light and blue light excitation.

The invention relates to the technical field of organic supramolecular chemical materials, in particular to an imine gelator with tri-state red light emission, a three-dimensional network-shaped gel material of the imine gelator, a preparation method and application of the imine gelator. The gel factor is an asymmetric cholesterol-phenoxy-maleonitrile-diethylamino salicyl gel factor, and can be self-assembled in different solvents to form three-dimensional structure gel of a short rod shape, a fiber net shape and a flat strip shape; due to the highly conjugated asymmetric molecular structure, the fluorescent probe presents three-state red fluorescence emission in a solid state, a solution state and a gel state; the gelator shows intuitive colorimetric change and fluorescence'on-off 'double-signal detection performance on Fe < 3 + > and Co < 2 + > in toluene gel, and has colorimetric change, fluorescence'on-off' and gel-to-sol conversion three-signal response on Cu < 2 + >; the gelator has fluorescence'on-off 'detection response to Cu < 2 + > in a solution state, and double-state four-detection response performance to Cu < 2 + > is achieved; the gelator-Cu < 2 + > binary system has fluorescent on-off detection potential on Pb < 2 + >.

This invention belongs to the field of luminescent materials technology, and discloses a green phosphor, its preparation method, and its application. The green phosphor has the chemical formula CaY. 1‑x GaO4:xCe 3+ Wherein, 0.02 ≤ x ≤ 0.2. The green phosphor of this invention, due to its specific composition, can be used not only in non-contact optical temperature measurement but also in indoor lighting. When applied to optical temperature measurement, it offers advantages such as rapid response, high resolution, high sensitivity, high temperature resistance, and no need for direct contact with objects. Furthermore, when mixed with blue LED chips and commercially available red phosphor, it can be used as a warm white LED device, which features low cost, stable performance, and good luminous effect.

The invention discloses a donor-receptor porphyrin molecular engineering-based high-sensitivity hypochlorous acid fluorescent probe and a synthesis method thereof, the structural formula of the probe is as follows: # imgabs0 #, the probe can detect hypochlorous acid (HClO) with high sensitivity, phenothiazine groups are introduced at two meso positions of porphyrin, and a donor-receptor (D-A) system is formed in molecules. The probe has rapid response performance to hypochlorous acid, sulfur atoms in phenothiazine are oxidized through hypochlorous acid, phenothiazine electrons are prevented from being transferred to porphyrin under light excitation, and therefore red fluorescence is shown, and selective detection of hypochlorous acid is achieved. After the probe reacts with hypochlorous acid, 667 nm red fluorescence is emitted under the excitation wavelength of 450 nm, the fluorescence appears obviously, and the Stokes shift is 217 nm. The reaction product has the advantages of good water solubility, high fluorescence quantum yield, large Stokes shift and the like. The probe can detect hypochlorous acid with high sensitivity, and has a huge application prospect in the technical fields of analytical chemistry, life science, environment detection, biomedical treatment and the like.

The invention discloses europium-doped hydrotalcite for enhancing PVC (polyvinyl chloride) light transmission and a preparation method and application thereof, and belongs to the technical field of high polymer materials and inorganic nanomaterials. Trivalent europium (Eu < 3 + >) is doped into a lattice structure of LDHs (layered double hydroxides) through europium-doped coprecipitation, surface silanization and ultrasonic dispersion technologies; refractive index matching (1.50-1.53) is achieved, and light scattering is reduced. The particle size of the optimized hydrotalcite is controlled to be 50-150 nm, so that the light transmittance of the PVC composite film reaches 93% or above, and meanwhile, the PVC composite film has the characteristic of red fluorescence. Compared with the prior art, the material has the advantages that the transparency is improved, the heat-resistant stability and aging resistance of PVC are remarkably improved, the material is suitable for the fields of transparent films, daylighting panels, optical marks and the like, and the hydrotalcite nano material provided by the invention has the dual effects of refractive index matching and conversion from ultraviolet light to visible light; the light transmittance and the ageing resistance of the PVC material can be obviously improved at the same time.

The invention provides an alternating magnetic field quantum sensing detection device and method, and relates to the technical field of alternating magnetic field quantum sensors. The device comprises a sensitive module which comprises a diamond and a diamond NV color center located in the diamond; the microwave module is used for generating a target microwave signal; the light path module comprises a laser, an optical fiber and a photoelectric detector; the laser is arranged at one end of the fiber; the diamond is arranged at the other end of the fiber; a target laser signal generated by the laser is transmitted to the diamond through the optical fiber, so that the NV color center of the diamond generates red fluorescence under the action of the target laser signal and the target microwave signal; the photoelectric detector is used for collecting red fluorescence output from one end of the optical fiber; and the signal processing module is connected with the photoelectric detector and is used for converting the red fluorescence from an optical signal to an electric signal and determining the magnetic field environment of the position where the sensitive module is located according to the converted electric signal.

The invention discloses a pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector as well as a construction method and application of the pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector. The construction method comprises the following steps: mixing an mCherry gene segment and an IRES2 gene segment which are respectively obtained by PCR (Polymerase Chain Reaction) amplification in equal mass, then carrying out fusion amplification to obtain an IRES2-mCherry fusion gene segment, and carrying out homologous recombination on the IRES2-mCherry fusion segment and a pCDNA-3xHA-hTERT linearization vector. On the basis of a pCDNA-3xHA-hTERT stent vector, a pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector with red fluorescence is successfully constructed, and the fusion expression vector can be used as a fusion expression vector of the pCDNA-3xHA-hTERT-IRES2. The construction method is simple and efficient, enzyme digestion is not needed, and the constructed pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector can be subsequently used for transfecting bovine endometrial epithelial cells, so that the immortalized bovine endometrial epithelial cells are obtained.

The invention belongs to the technical field of medicines, and discloses a near-infrared fluorescent probe taking an alpha, beta-unsaturated acryloyl ester unit as a response site of sulfydryl. The fluorescent probe has great advantages in the aspects of biological small molecule identification, protein marking and disease diagnosis, and is one of the most convenient and most popular tools for exploring a complex biological process. The fluorescent probe molecule capable of releasing red fluorescence after being activated by sulfydryl is obtained for the first time by connecting a fluorophore with an alpha, beta-unsaturated acryloyl ester unit. The fluorescent probe molecule disclosed by the invention can be used for specifically detecting sulfydryl in an organism, so that the aim of accurately monitoring the sulfydryl in a cell is fulfilled.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a ratio fluorescent probe based on red / blue double-color carbon dots as well as a preparation method and application of the ratio fluorescent probe. The ratio fluorescent probe based on the red-blue double-color carbon dots is obtained by mixing a blue fluorescent carbon dot solution and a red fluorescent carbon dot solution according to the volume ratio of 6: 1, and the concentrations of the blue fluorescent carbon dot solution and the red fluorescent carbon dot solution are both 0.5 mg / mL; the blue fluorescent carbon dot solution is prepared by the following steps: adding deionized water into a mixed solution of citric acid and ethidene diamine, carrying out ultrasonic treatment, and then carrying out reaction centrifugation, filtration and dialysis to obtain blue fluorescent carbon dots; the red fluorescent carbon dot solution is prepared by adding deionized water and concentrated sulfuric acid into o-phenylenediamine, L-cysteine and boric acid, carrying out ultrasonic treatment, reacting, centrifuging, filtering, dialyzing and freeze-drying. The ratio fluorescent probe based on the red / blue double-color carbon dots can accurately detect the concentration of quercetin in the concentration range of 0-30.0 [mu] mol / L.

The invention discloses application of a fluorescent probe in simultaneous imaging of lysosome and nucleolus and nucleic acid detection, and belongs to the technical field of fluorescent probes. The invention discloses (E)-2-(4-(ethylamino)-2-hydroxyvinyl)-3-(2-ethoxyl) benzothiazole-3-iodised salt as a fluorescent probe capable of imaging lysosome and nucleolus at the same time, and as a fluorescent probe for detecting nucleic acid. The probe provided by the invention is a red fluorescent probe, which is helpful for imaging microstructures of cells and tissues. The lysosome and the nucleolus are closely related to diagnosis of various diseases (cancers and the like) and drug research and development. Therefore, the fluorescent compound provided by the invention can provide a more accurate and effective tool for research and application in the fields of chemical industry, biological medicine and the like.