325 results about "Red fluorescence" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention discloses an MOF-CQD enzyme-sensitive FRET hydrogel diagnosis and treatment probe as well as a preparation method and application thereof, and belongs to the technical field of biomedical detection and treatment. The probe takes a carboxyl carbon quantum dot (CQD) as a donor and a metal organic framework (MOF) as an acceptor, and the donor and the acceptor are connected through a replaceable enzyme response polypeptide bridge; when the fluorescence is complete, FRET is generated to quench the CQD fluorescence, the FRET is interrupted after the target enzyme cuts off the peptide bridge, and the blue / red fluorescence ratio is changed to realize nanomole-level quantitative detection and has self-correction capability. The probe is packaged in a four-arm PEG transparent hydrogel which does not need a photoinitiator and can be quickly self-crosslinked, and can be prepared into a film, a tooth socket and the like for POCT (point-of-care testing) of parts such as an oral cavity / skin and the like. After detection, the MOF is activated by illumination of 630-660 nm to generate active singlet oxygen, so that local antibacterial or anti-tumor treatment is realized, and a diagnosis and treatment integrated scheme is constructed.

The invention is about a method of marking ink which is only used to defend fake. The composition of it is: lipidol or lipid, surface active agent, integration agent, water, buffering agent. Their mass percents are: 1,2-hexylene glycol: 5%-15%, butanol: 1-5%, DH-1: 5-10%, EDTA: 0.1-0.5%, MS-1: 0.1-1%, water: the rest. The process is: a. dissolving the colorant in water, b. put the correct percent of 1,2-hexylene glycol and butanol into the solvent, then dropping the MS-1 and EDTA solution when mixing the solvent for 30-50 min, c. put the rest precent of water into the printing ink box. The picture and word would be shown red fluorescence in the UV but blank in the usual light.

A phosphor composition includes a red phosphor material having a red decay rate and a green phosphor material having a green decay rate. The red phosphor material includes a Mn4+ doped phosphor of Formula I and a Eu3+ doped uranium phosphor, and a difference between the red decay rate and the green decay rate is no more than 7 ms, AxMFy:Mn4+ (I), wherein A is Li, Na, K, Rb, Cs, or a combination thereof; M is Si, Ge, Sn, Ti, Zr, Al, Ga, In, Sc, Hf, Y, La, Nb, Ta, Bi, Gd, or a combination thereof; x is an absolute value of a charge of the MFy ion; and y is 5, 6 or 7. A device is also provided.

The invention discloses a diamond low-frequency current measurement method with nonlinear response to magnetic field intensity, which is characterized in that two coils are wound on a magnetic flux collector with a communicated structure, a high-frequency (800Hz) high-voltage (7V) driving current is injected into one of the coils, a magnetic field generated by the coil is amplified through the magnetic flux collector and guided to an area where an NV color center magnetometer is located, and the NV color center magnetometer is located; and the signal is driven to enter a nonlinear response interval in the demodulated ODMR spectrum. A weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal is injected into the other coil, amplitude modulation is performed on the weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal by using the same frequency as the driving current, and the weak (1-50mV) to-be-measured low-frequency (0.1-10Hz) current signal is amplified by the magnetic flux collector and guided to the NV The microwave frequency is locked at the maximum slope point of the ODMR frequency spectrum through CW-ODMR phase-locked amplification, so that the relationship between the change of the red fluorescence signal intensity and the size of the external magnetic field is obtained, and the signal intensity of the low-frequency current magnetic field to be measured is measured according to the relationship.

The invention relates to a dental plaque detection method and system based on ultraviolet image analysis. The method comprises the following steps: acquiring an RGB image of dental plaque; converting the RGB image into an HSV image; performing tooth region identification, single tooth segmentation and single tooth contour cutting on the HSV image in sequence to obtain each single tooth ROI pixel and a total tooth ROI pixel; extracting a red fluorescence characteristic region from the HSV image, and calculating to obtain a red fluorescence region pixel and a red fluorescence average brightness value; the dental plaque severity score is calculated according to the total tooth ROI pixel, the red fluorescence region pixel and the red fluorescence average brightness value, the severity of the dental plaque is quantified, a user can know the tooth health state more clearly, and then oral health is maintained. The change of the score value is beneficial to motivating the user to continuously monitor the dental plaque condition, so that the user can conveniently manage oral hygiene and health more finely, and the user experience is improved.

The present invention relates to a dual-probe detection reagent and a preparation method for detecting Salmonella typhimurium, and belongs to the field of food safety technology. The dual-probe detection reagent is composed of a magnetic separation probe reagent solution and a fluorescent nanozyme probe reagent solution, which are packaged separately; the magnetic separation probe reagent solution is used to specifically bind to Salmonella typhimurium and separate and enrich the test sample; the red fluorescent nanozyme probe reagent solution is used to specifically bind to the Salmonella typhimurium after separation and enrichment, and generate a Salmonella typhimurium concentration-dependent colorimetric signal and a fluorescent signal. The dual-probe detection reagent of the present invention has a short detection time and high detection sensitivity for the detection of Salmonella typhimurium, and has good specificity for common model strains. The present invention has a high specific surface area and excellent peroxidase-like activity, which enhances the high-sensitivity capture efficiency; the dual-modal signals of the present invention verify each other, play an internal proofreading role, and the detection results are accurate.

The application provides a manganese-activated antimony acid-based fluorescent powder and a preparation method and application thereof, and relates to the technical field of luminescent materials. 1‑ x Y x )3Li5Sb 2‑y O 12 :yMn, and 0 < x <= 0.3, 0.001 <= y <= 0.01. 3+ The Y 3+ can improve the luminous efficiency and thermal stability of the fluorescent powder, and the fluorescent powder can emit red fluorescence with a wave band of 650nm-770nm under near-ultraviolet light and blue light excitation.

The invention relates to the technical field of organic supramolecular chemical materials, in particular to an imine gelator with tri-state red light emission, a three-dimensional network-shaped gel material of the imine gelator, a preparation method and application of the imine gelator. The gel factor is an asymmetric cholesterol-phenoxy-maleonitrile-diethylamino salicyl gel factor, and can be self-assembled in different solvents to form three-dimensional structure gel of a short rod shape, a fiber net shape and a flat strip shape; due to the highly conjugated asymmetric molecular structure, the fluorescent probe presents three-state red fluorescence emission in a solid state, a solution state and a gel state; the gelator shows intuitive colorimetric change and fluorescence'on-off 'double-signal detection performance on Fe < 3 + > and Co < 2 + > in toluene gel, and has colorimetric change, fluorescence'on-off' and gel-to-sol conversion three-signal response on Cu < 2 + >; the gelator has fluorescence'on-off 'detection response to Cu < 2 + > in a solution state, and double-state four-detection response performance to Cu < 2 + > is achieved; the gelator-Cu < 2 + > binary system has fluorescent on-off detection potential on Pb < 2 + >.

This invention belongs to the field of luminescent materials technology, and discloses a green phosphor, its preparation method, and its application. The green phosphor has the chemical formula CaY. 1‑x GaO4:xCe 3+ Wherein, 0.02 ≤ x ≤ 0.2. The green phosphor of this invention, due to its specific composition, can be used not only in non-contact optical temperature measurement but also in indoor lighting. When applied to optical temperature measurement, it offers advantages such as rapid response, high resolution, high sensitivity, high temperature resistance, and no need for direct contact with objects. Furthermore, when mixed with blue LED chips and commercially available red phosphor, it can be used as a warm white LED device, which features low cost, stable performance, and good luminous effect.

The invention provides an alternating magnetic field quantum sensing detection device and method, and relates to the technical field of alternating magnetic field quantum sensors. The device comprises a sensitive module which comprises a diamond and a diamond NV color center located in the diamond; the microwave module is used for generating a target microwave signal; the light path module comprises a laser, an optical fiber and a photoelectric detector; the laser is arranged at one end of the fiber; the diamond is arranged at the other end of the fiber; a target laser signal generated by the laser is transmitted to the diamond through the optical fiber, so that the NV color center of the diamond generates red fluorescence under the action of the target laser signal and the target microwave signal; the photoelectric detector is used for collecting red fluorescence output from one end of the optical fiber; and the signal processing module is connected with the photoelectric detector and is used for converting the red fluorescence from an optical signal to an electric signal and determining the magnetic field environment of the position where the sensitive module is located according to the converted electric signal.

The invention discloses a pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector as well as a construction method and application of the pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector. The construction method comprises the following steps: mixing an mCherry gene segment and an IRES2 gene segment which are respectively obtained by PCR (Polymerase Chain Reaction) amplification in equal mass, then carrying out fusion amplification to obtain an IRES2-mCherry fusion gene segment, and carrying out homologous recombination on the IRES2-mCherry fusion segment and a pCDNA-3xHA-hTERT linearization vector. On the basis of a pCDNA-3xHA-hTERT stent vector, a pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector with red fluorescence is successfully constructed, and the fusion expression vector can be used as a fusion expression vector of the pCDNA-3xHA-hTERT-IRES2. The construction method is simple and efficient, enzyme digestion is not needed, and the constructed pCDNA-3xHA-hTERT-IRES2-mCherry fusion expression vector can be subsequently used for transfecting bovine endometrial epithelial cells, so that the immortalized bovine endometrial epithelial cells are obtained.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a ratio fluorescent probe based on red / blue double-color carbon dots as well as a preparation method and application of the ratio fluorescent probe. The ratio fluorescent probe based on the red-blue double-color carbon dots is obtained by mixing a blue fluorescent carbon dot solution and a red fluorescent carbon dot solution according to the volume ratio of 6: 1, and the concentrations of the blue fluorescent carbon dot solution and the red fluorescent carbon dot solution are both 0.5 mg / mL; the blue fluorescent carbon dot solution is prepared by the following steps: adding deionized water into a mixed solution of citric acid and ethidene diamine, carrying out ultrasonic treatment, and then carrying out reaction centrifugation, filtration and dialysis to obtain blue fluorescent carbon dots; the red fluorescent carbon dot solution is prepared by adding deionized water and concentrated sulfuric acid into o-phenylenediamine, L-cysteine and boric acid, carrying out ultrasonic treatment, reacting, centrifuging, filtering, dialyzing and freeze-drying. The ratio fluorescent probe based on the red / blue double-color carbon dots can accurately detect the concentration of quercetin in the concentration range of 0-30.0 [mu] mol / L.

The invention discloses a method for applying photoswitch fluorescent polymers to dynamic fluorescent falsification prevention, which comprises the following steps: taking methyl acrylate, 1, 6-hexanediol diacrylate, a photoinitiator 819, a green fluorescent dye BDPMA and one of three photochromic spiropyrans SPMA, SPAMA and SPBMA as raw materials, and preparing three photoswitch fluorescent polymers P1, P2 and P3 in a photopolymerization manner. The P1, P2 and P3 are irradiated by ultraviolet light and placed in room-temperature darkness, red fluorescence generated by the P1, P2 and P3 can be gradually and spontaneously converted into green fluorescence, due to the structural difference of the three spiropyrans, the fluorescence discoloration rates of the three polymers show obvious difference, and a new dynamic fluorescent anti-counterfeit label is constructed by utilizing the difference. The photoswitch fluorescent polymer obtained by the invention has the advantages of simple preparation method, stable structure and good machinability, is suitable for amplification synthesis and practical application, and has wide application prospects in the fields of optical anti-counterfeiting and information encryption.

The invention relates to the related technical field of molecular biology and cell engineering, in particular to an FLP-IN-PIEC cell line and a construction method and application thereof, and the construction method comprises the following steps: step 1, constructing CRISPR / Cas9 site-specific cleavage plasmid containing a pig HIPP11 safety site sequence; step 2, constructing a donor plasmid Donor-FRT containing a homologous arm of a safety site of the HIPP11; 3, jointly transfecting plasmids in the step 1 and the step 2 into a porcine hip artery endothelial cell line (PIEC) to obtain an FLP-IN-PIEC cell line for expressing red fluorescence; and 4, under the action of Flp recombinase, transfecting an exogenous gene donor plasmid containing an FRT site to the FLP-IN-PIEC cell line obtained in the step 3, and driving the expression of a target gene through an optimized strong promoter to realize fixed-point transfer and efficient expression of the exogenous gene in the pig (PIEC) cell line. Different from an existing Flp-In system depending on a random integration strategy, the construction method disclosed by the invention emphasizes fixed-point integration and promoter optimization, and improves the homogeneity, stability and screening efficiency of exogenous gene expression.

The invention discloses a nopinic alkyl acridine fluorescent probe for detecting hydrazine and viscosity as well as a preparation method and application of the nopinic alkyl acridine fluorescent probe. The probe is 2-(2-(5-(4-(3, 3-dimethyl-1, 2, 3, 4-tetrahydro-2, 4-methylene acridine-9-yl) phenyl) furan-2-yl) vinyl)-1, 1, 3-trimethyl-1H-benzo [e] indoleonium iodized salt, which is called TA-PFV-BI for short. The probe can detect the viscosity of a solution, and red fluorescence emitted by the probe is gradually enhanced along with the improvement of the viscosity of the solution under the irradiation of ultraviolet light with the wavelength of 365 nm. The probe can also have a specific reaction with hydrazine, so that the fluorescence color of a probe solution is changed from colorless to bright blue, the detection range is 0-90 mu M, the response time is within 5 seconds, the detection limit is 4 * 10 <-8 > M, and the applicable pH range is 5-10. The probe TA-PFV-BI, as a bifunctional fluorescent probe capable of simultaneously detecting hydrazine and viscosity, has the advantages of good selectivity, high sensitivity, fast response, wide application range and the like, and shows a good application prospect.

This invention provides a method for constructing a fluorescently traced mouse model and its application in the sorting of Mcpt4-positive and negative mast cells. First, the Cre gene is knocked into the Mcpt4 gene sequence of C57BL / 6 mice using CRISPR / Cas9 technology. Then, through animal hybridization and Cre-loxP recombination technology, a fluorescently traced mouse model of Mcpt4-positive mast cells is obtained. The fluorescently traced mouse model possesses a dual-fluorescent reporter gene system consisting of the red fluorescent protein tdTomato and the green fluorescent protein ZsGreen. Mcpt4-positive mast cells exhibit red fluorescence, while other Mcpt4-negative cells exhibit green fluorescence. Using this fluorescently traced mouse model, Mcpt4-positive and negative mast cells can be rapidly, accurately, and effectively sorted, providing a reliable technical basis for exploring the roles and mechanisms of Mcpt4-positive and negative mast cells in immune responses.

The present invention belongs to the field of biological detection technology, and in particular relates to a rapid and accurate detection method for pathogens based on lanthanide MOF and paper needles. The present invention constructs a fluorescent biosensor by combining LnMOF with an aptamer (Apt-LnMOF) that specifically captures target pathogens. A needle-shaped paper-based device (paper needle) is used as a visual signal output carrier, and qualitative or quantitative analysis is performed by the diffusion distance and red fluorescence intensity (R value) of Apt-LnMOF at the paper-based interface. Apt-LnMOF is used as a signal label for a visualization detection strategy to improve the sensitivity and response speed of the visualization detection method for pathogens; the dual signal coupled with the diffusion distance and R value of Apt-LnMOF at the paper-based interface is used to visualize the result output, thereby improving the accuracy of the detection method. The present invention uses paper needles as signal output carriers, which improves the convenience of operation for non-professionals.

The invention discloses a multi-emission composite nano ratiometric fluorescent probe for detecting fluoroquinolone antibiotics and a preparation method of the multi-emission composite nano ratiometric fluorescent probe. The CdTe-Tb-UMP composite nano ratiometric fluorescent probe with double ligands is prepared by taking metal terbium ions (Tb < 3 + >) as central ions and respectively taking uridine monophosphate (UMP) and CdTe quantum dots with red fluorescence emission as bridged ligands. ENR is further coordinated with Tb < 3 + >, energy is transferred to Tb < 3 + > through an antenna effect, so that characteristic green fluorescence of Tb < 3 + > is turned on, and fluorescence of CdTe quantum dots is almost kept unchanged. On the basis, a portable nursing point testing system is further constructed, and the portable nursing point testing system comprises an intelligent mobile phone detector and a wearable fingertip sensor integrated with a micro-fluidic chip and is used for on-site rapid detection of the ENR. The paper-based chip shows rapid and sensitive fluorescence response, and the detection limits of the spectrograph and the paper-based sensor on the ENR are 4.7 nM and 13.1 nM respectively. The probe can be applied to detection of ENR in actual environment water samples, food and drugs.

The present disclosure relates to a phosphor compound for diagnosing fire blight in fruit trees based on fluorescence and use thereof. The compound is capable of selectively detecting Erwinia amylovora, the causal pathogen of fire blight in fruit trees, and emits red fluorescence in response. Due to its rapid diagnostic response and high sensitivity, the compound can be effectively used for diagnosing fire blight in fruit trees through various simple methods such as spraying or swabbing, without the need for specialized equipment.

The invention discloses a novel BODIPY-based near-infrared open-type fluorescent probe for detecting hypochlorous acid as well as a preparation method and application of the novel BODIPY-based near-infrared open-type fluorescent probe. The fluorescent probe is (5, 5-difluoro-1, 3, 9-trimethyl-7-(4-sulfur morpholinyl styryl)-5H-dipyrrolo-diazaborolo-penta [10] alkene-10-yl)-4-phenol, which is abbreviated as BOD-Y. The invention also discloses a preparation method of the fluorescent probe. The probe BOD-Y can specifically recognize hypochlorous acid, the color of a probe BOD-Y solution added with hypochlorous acid is rapidly changed from green to blue under sunlight, and the fluorescence is also changed from colorless to strong red fluorescence. The detection limit of the probe on hypochlorous acid is as low as 21nM, the probe shows a good linear relation within 0-35 [mu] M, and the response time is within 13 seconds. The probe shows good selectivity on hypochlorous acid, and is not influenced and interfered by other anions, cations, biological mercaptan, active oxygen and the like.

The invention belongs to the technical field of medical instruments, and particularly relates to a medical multifunctional operation pasting film which is characterized in that the medical multifunctional operation pasting film comprises a sterile basement film, an anatomical structure body surface projection layer is arranged on the surface of the sterile basement film, and the anatomical structure body surface projection layer comprises a nerve and blood vessel identification area, a tissue level identification area and an operation path guiding area; an anti-curling adhesive tape is arranged at the edge of the sterile base film, and release paper is fixedly connected to the back surface of the sterile base film. In the anatomical structure body surface projection layer, the nerve and blood vessel identification area is printed through vegetable dye, blood vessels and nerves are clearly distinguished through red fluorescent dye and blue fluorescent dye under ultraviolet light, and positioning errors caused by traditional hand drawing are avoided; protruding texture of the tissue level identification area assists a surgeon in judging the dissection depth through touch feedback, scale marks and an erasable coating of the operation path guiding area support accurate measurement and personalized marking in the operation, and the risk of important tissue damage is reduced.

The invention provides aluminate dark red fluorescent powder as well as a preparation method and application thereof, and belongs to the technical field of solid luminescent materials. According to the aluminate dark red fluorescent powder, manganese is adopted to activate aluminate, the excitation spectrum coverage area is wide, the excitation range covers most of ultraviolet and blue light areas, and the emission spectrum is concentrated near 659nm. The fluorescent powder can absorb light from ultraviolet light to a blue light region in a solar spectrum, has very strong emission intensity in a dark red light region, can emit red light under the irradiation of sunlight, can improve the utilization rate of light energy, and is more favorable for promoting the photoelectric conversion efficiency of a silicon solar cell. The fluorescent powder can be prepared into a film to be compounded on the surface of a solar cell, the solar spectrum is optimized, and the energy conversion efficiency of the solar cell is improved.

The application discloses a fluorescent double-emission ssDNA-silver cluster probe for rapidly and visually detecting Hg 2+ and a preparation method thereof. The application discloses a fluorescent double-emission ssDNA-silver cluster probe for rapidly and visually detecting Hg 2+ , which contains a template ssDNA capable of simultaneously emitting strong red fluorescence and weak green fluorescence. The sequence of the ssDNA is 5'-AACAAAGCCCCCCCCCCCCCTTTTTTTTTT-3'. The fluorescent double-emission ssDNA-silver cluster probe can rapidly and visually detect Hg 2+ . The ssDNA-silver cluster probe has excellent selectivity for Hg 2+ , and has been used for rapid detection of trace Hg 2+ in actual water samples, and the detection result is sensitive and accurate.

The application discloses a kind of Mn 4+ Activated fluorine (oxygen) oxide red fluorescent material and its preparation method;The application uses organic-inorganic hybrid fluoride manganese salt material as Mn 4+ Source preparation Mn 4+ Activated fluorine (oxygen) oxide red fluorescent material.The application uses organic-inorganic hybrid fluoride manganese salt material instead of all-inorganic fluoride manganese salt, manganese salt and permanganate as Mn 4+ Source, for preparing Mn 4+ Activated fluorine (oxygen) oxide red fluorescent material, significantly improve the luminescent efficiency of Mn 4+ Activated fluorine (oxygen) oxide red fluorescent material.

The application relates to the technical field of light-emitting materials, and discloses a red fluorescent glass ceramic material, which has a general chemical formula of xMgO:yAl2O3:zSiO2:mEu 2+ Wherein, x, y, z and m respectively represent the mole fraction of corresponding elements, and 0.5<=x<=3, 0.5<=y<=3.5, 2<=z<=6 and 0.005<=m<=0.08; the red fluorescent glass ceramic material is prepared by laser sintering and reduction heat treatment. The preparation method is short in time consumption and low in energy consumption; the prepared red fluorescent glass ceramic has excellent light-emitting performance under blue light excitation and can be directly used as a fluorescent conversion element.

This invention belongs to the field of biotechnology and discloses a dry film stabilizer, cytoplasmic antigen dry films, their preparation methods, and applications. The dry film stabilizer includes polyethylene glycol, trehalose, BSA, melatonin, and Tween-20. Cytoplasmic antigen dry films are prepared using this stabilizer and applied in CBA detection. This dry film stabilizer has good permeability, penetrating the cell membrane to enter the cell interior, providing protection both inside and outside the cell. It also possesses moisturizing and antioxidant properties, preventing cell deformation and shedding of the cell fixation layer, effectively maintaining the stability and reliability of intracellular antigen proteins. The prepared cytoplasmic antigen dry films can be stored long-term without damaging the antigenicity of cytoplasmic signal indicators. When detecting positive samples, strong and numerous green filamentous fluorescence signals are observed in the cytoplasm, with consistent green and red fluorescence intensities. Software synthesis reveals co-localization of green and red antigen fluorescence.

The invention relates to a preparation method of rare earth metal gadolinium doped red carbon dots, and belongs to the technical field of nano material synthesis and biomedical imaging. Aiming at the problems of low gadolinium ion doping efficiency, more impurity residues and single function in the existing carbon dot preparation, the method comprises the following steps: dissolving p-phenylenediamine and dicyandiamide in a specific molar ratio, adding a GdCl solution, and carrying out ultrasonic dispersion to form a uniform system; performing hydrothermal reaction by adopting a staged temperature control program to optimize the crystallinity of the carbon matrix; high-efficiency removal of impurities is realized through high-speed centrifugation, microporous filtration and molecular weight interception dialysis; and finally, performing low-temperature vacuum drying to obtain a powder product. The obtained gadolinium-doped red carbon dots have the characteristics of uniform particle size, abundant surface hydrophilic functional groups, stable red fluorescence emission and synergistic enhancement of magnetic responsiveness, and can be used for constructing a bimodal fluorescence-magnetic resonance imaging and diagnosis and treatment integrated platform in a living body.

The invention discloses a Mn < 4 + >-doped rubidium lithium fluorogallate monocrystal red-light-emitting material as well as a preparation method and application thereof. The Mn < 4 + >-doped rubidium lithium fluorogallate single-crystal red-light-emitting material is a single-crystal material, and the crystal grain size is 0.01-2 mm. The chemical formula of the material is Rb2LiGaF6: xMn < 4 + >, wherein x is equal to 0.5-9%. A fluoride single-crystal red light material is prepared by adopting a saturated solution crystallization method, and the Mn < 4 + > doped rubidium lithium fluorogallate single-crystal red light material, a yellow light emitting material or a green light emitting material and a blue light InGaN chip are combined and packaged to obtain a white light LED device. The Mn < 4 + >-doped rubidium lithium fluorogallate monocrystal red light material prepared by the invention can be efficiently activated by purple light and blue light, the strongest excitation wavelength is 467 nm, red fluorescence with high brightness and high color purity is emitted, the color purity is 99.9%, the strongest emission wavelength is 627 nm, the full width at half maximum is 11.3 nm, the color coordinate x is greater than 0.7, and the fluorescence lifetime is 3.44 ms. The fluorescent material can be used for a wide-color-gamut display device, reaches 109.7% NTSC, and is superior to a commercial K2SiF6: Mn < 4 + > packaged device.

The invention discloses a carbon nanodot as well as a preparation method and application thereof, and relates to the technical field of carbon nanomaterials. The carbon nanodots are prepared from raw materials including nicotinamide, urea and citric acid or a derivative thereof through solvent heat treatment, and the derivative of the citric acid is selected from citrate, a citric acid hydrate or a hydrate of the citrate. According to the invention, nicotinamide is used as a precursor to synthesize the carbon nanodot under solvent heat treatment, so that the carbon nanodot integrates intrinsic biological activity, efficient photothermal conversion capability and red fluorescence imaging function at the same time, and the limitation of a single therapy is overcome through multifunctional synergistic therapy. In addition, the carbon nanodot provided by the invention has extremely high biological safety.