6 results about "Tachypleus" patented technology

The invention discloses a reaction buffer solution for improving endotoxin detection sensitivity of a recombinant tachypleus amebocyte lysate, a kit containing the reaction buffer solution and application of the reaction buffer solution. The reaction buffer solution comprises the following components: a Tris-HCl buffer solution, magnesium ions, sodium chloride, cane sugar, ethylene glycol diethyl ether diamine tetraacetic acid, a nonionic surfactant and cyclodextrin or a derivative thereof. The invention also discloses a preparation method of the reaction buffer solution and a kit containing the reaction buffer solution. When the reaction buffer solution or the kit is used for detecting the endotoxin of the recombinant tachypleus amebocyte lysate, the sensitivity of the recombinant tachypleus amebocyte lysate for detecting the endotoxin can be improved by one order of magnitude, the lowest detection limit can reach 0.0005 EU / ml, the reaction buffer solution or the kit has strong anti-interference capability and short reaction time, and the detection efficiency is greatly improved.

The invention discloses a method for eliminating interference in LNP type sample bacterial endotoxin detection. The method comprises the following steps: S1, determining a detection standard curve of a tachypleus amebocyte lysate dynamic color development method; s2, preparing a test sample solution A with different dispersing agent volume ratios and a test sample solution B added with the endotoxin with the midpoint concentration in the standard curve, and carrying out an interference elimination test by adopting the standard bacterial endotoxin solution C, the solution A and the solution B; s3, redissolving the tachypleus amebocyte lysate with bacterial endotoxin inspection water according to the specification of the tachypleus amebocyte S4, detecting by adopting a tachypleus amebocyte lysate dynamic color development method; and S5, judging the effectiveness of the experiment. According to the method for eliminating interference in bacterial endotoxin detection of the LNP type sample, the endotoxin dispersing agent is introduced, so that the multi-dimensional interference problems of endotoxin wrapped by lipid in the LNP sample, pH value deviation, osmotic pressure abnormity and the like are solved in a targeted manner.

The invention discloses a method for detecting bacterial endotoxin in arginine hydrochloride. The method comprises the following steps: rechecking sensitivity of tachypleus amebocyte lysate; preparing a test solution; gradient dilution; interference test; and detecting by a gel method. According to the arginine hydrochloride bacterial endotoxin detection method, interference is effectively eliminated through pH value adjustment and a gradient dilution method, and an effective and reliable detection method is provided for arginine hydrochloride bacterial endotoxin detection.

This invention discloses a method for surveying juvenile horseshoe crabs in China based on remote sensing technology and intelligent image recognition. Through a process of rapid comprehensive survey and precise regional survey, a drone equipped with a lightweight YOLO recognition model, RTK positioning, and laser ranging is used to conduct a comprehensive survey of potential habitats, identify juvenile distribution hotspots, and delineate precise survey areas. Then, a full-frame mapping camera is used to conduct ultra-high-resolution drone photogrammetry, combined with an instance segmentation model to complete juvenile contour extraction, coordinate positioning, density statistics, and age determination. This invention overcomes the shortcomings of traditional foot surveys, such as tidal limitations, small coverage area, susceptibility to environmental interference, high missed detection rate, and high personnel risk. It achieves non-contact, high-efficiency, high-precision, and low-disturbance juvenile horseshoe crab surveys, providing a scientific and efficient technical solution for the monitoring of juvenile horseshoe crabs in China and the protection of endangered species.

The present disclosure relates generally to compositions, dosage forms, and methods for the prevention and treatment of cancer and infections. One example method entails administering to a patient an effective amount of a composition comprising 1 * 107 to 500 * 107 intact, stable and substantially inactive Escherichia coli cells that have been treated to result in an untreated Escherichia coli cell, when measured by a tachypleus amebocyte lysate (LAL) assay, as compared to an untreated wild-type Escherichia coli cell. Lipopolysaccharide (LPS)-related endotoxin activity is reduced by about 70% to 99%, and wherein the composition contains LPS of 124 to 62000 endotoxin units (EU).

The invention discloses an edaravone raw material bacterial endotoxin inspection method, and belongs to the technical field of medicine quality control. According to the method, a gel method tachypleus amebocyte lysate is taken as a core, and the interference of an edaravone molecular pyrazolone group and a residual intermediate on detection is effectively eliminated by optimizing the dilution concentration of a test sample, establishing a gradient dilution interference test system, treating a turbid sample by combining a 0.22 mu m pyrogen-free filter membrane, retesting double-batch reagent verification and other key technologies. Triple quality control is set to ensure reaction effectiveness, complex instrument analysis is not needed, whether bacterial endotoxin is qualified or not can be accurately judged, the blank of exclusive detection of edaravone raw material endotoxin is filled, and the method is suitable for industrial quality control.