11 results about "Tachypleus" patented technology

The invention discloses a reaction buffer solution for improving endotoxin detection sensitivity of a recombinant tachypleus amebocyte lysate, a kit containing the reaction buffer solution and application of the reaction buffer solution. The reaction buffer solution comprises the following components: a Tris-HCl buffer solution, magnesium ions, sodium chloride, cane sugar, ethylene glycol diethyl ether diamine tetraacetic acid, a nonionic surfactant and cyclodextrin or a derivative thereof. The invention also discloses a preparation method of the reaction buffer solution and a kit containing the reaction buffer solution. When the reaction buffer solution or the kit is used for detecting the endotoxin of the recombinant tachypleus amebocyte lysate, the sensitivity of the recombinant tachypleus amebocyte lysate for detecting the endotoxin can be improved by one order of magnitude, the lowest detection limit can reach 0.0005 EU / ml, the reaction buffer solution or the kit has strong anti-interference capability and short reaction time, and the detection efficiency is greatly improved.

The invention discloses an endotoxin concentration quantitative detection device and method, and relates to the field of medical detection.The device comprises a light source, an optical fiber Raman sensor, a capillary tube and a signal detection unit; the light source is used for emitting light signals to the optical fiber Raman sensor; the capillary tube is used for packaging the optical fiber Raman sensor and sucking a to-be-detected liquid sample, so that the to-be-detected liquid sample is in contact with the optical fiber Raman sensor; the liquid sample to be detected contains tachypleus amebocyte lysate protein and endotoxin; endotoxin is combined with tachypleus amebocyte lysate protein, so that the adsorption effect of the surface of the optical fiber Raman sensor on the tachypleus amebocyte lysate protein is reduced, an optical signal is scattered, and a Raman fingerprint characteristic spectrum is formed; the signal detection unit is used for determining the endotoxin concentration of the liquid sample to be detected according to the intensity change of the Raman fingerprint characteristic spectrum. According to the invention, the detection reliability is ensured, and meanwhile, the use amount of limulus lysate protein is reduced.

The invention discloses a method for eliminating interference in LNP type sample bacterial endotoxin detection. The method comprises the following steps: S1, determining a detection standard curve of a tachypleus amebocyte lysate dynamic color development method; s2, preparing a test sample solution A with different dispersing agent volume ratios and a test sample solution B added with the endotoxin with the midpoint concentration in the standard curve, and carrying out an interference elimination test by adopting the standard bacterial endotoxin solution C, the solution A and the solution B; s3, redissolving the tachypleus amebocyte lysate with bacterial endotoxin inspection water according to the specification of the tachypleus amebocyte S4, detecting by adopting a tachypleus amebocyte lysate dynamic color development method; and S5, judging the effectiveness of the experiment. According to the method for eliminating interference in bacterial endotoxin detection of the LNP type sample, the endotoxin dispersing agent is introduced, so that the multi-dimensional interference problems of endotoxin wrapped by lipid in the LNP sample, pH value deviation, osmotic pressure abnormity and the like are solved in a targeted manner.

The invention discloses a bacterial biosensor as well as a preparation method and application thereof. According to the bacterial biosensor, glass (BK7) with the surface covered with gold nanoparticles serves as a substrate, the surfaces of the gold nanoparticles are coupled and modified with specific antibodies, and the gold nanomaterial is prepared and applied to detection of bacterial endophytic ophthalmia. Gold nanoparticles are taken as a substrate, a high-sensitivity, selective and label-free method for simultaneously detecting gram-positive (G +) and gram-negative (G-) bacteria in a sample is constructed, small sample size requirements and short experiment operation time are met, and when the method is used for detecting the bacteria, the use of tachypleus amebocyte lysate can be avoided; the method has the advantages that the method is simple and convenient to operate, can effectively distinguish G < + > and G <-> bacteria, is more efficient to use, is a more humanized and ecologically sustainable bacteria detection method, plays an important role in protecting the horseshoe crabs, and is convenient to popularize and use.

The invention discloses a method for detecting gentisic acid bacterial endotoxin, which comprises the following steps: (1) preparing a test solution: taking a gentisic acid sample to be detected, adding BET water, and then adding a sodium hydroxide solution to prepare the test solution; a to-be-detected product mixed solution: adding the gentisic acid bacterial endotoxin standard solution into the obtained to-be-detected product solution to obtain the to-be-detected product mixed solution; (2) interference pre-screening experiment: respectively adding tachypleus amebocyte lysate into the test solution and the mixed solution of the to-be-detected product for reaction to obtain the concentration of the test solution corresponding to a second positive result; and (3) formal interference experiment: taking the concentration of the test solution corresponding to the second positive result obtained by the interference pre-screening experiment as a standard, and carrying out the interference experiment according to the description in the general rule 1143 of the four parts of the Chinese pharmacopoeia 2020 edition. The method provided by the invention can effectively solve the problem that the sample cannot be dissolved in water, and meanwhile, the sample is directly adjusted from an acid solution to neutral and slightly alkaline, so that the interference of the sample on the tachypleus amebocyte lysate can be eliminated.

The invention discloses a method for detecting bacterial endotoxin in arginine hydrochloride. The method comprises the following steps: rechecking sensitivity of tachypleus amebocyte lysate; preparing a test solution; gradient dilution; interference test; and detecting by a gel method. According to the arginine hydrochloride bacterial endotoxin detection method, interference is effectively eliminated through pH value adjustment and a gradient dilution method, and an effective and reliable detection method is provided for arginine hydrochloride bacterial endotoxin detection.

This invention discloses a method for surveying juvenile horseshoe crabs in China based on remote sensing technology and intelligent image recognition. Through a process of rapid comprehensive survey and precise regional survey, a drone equipped with a lightweight YOLO recognition model, RTK positioning, and laser ranging is used to conduct a comprehensive survey of potential habitats, identify juvenile distribution hotspots, and delineate precise survey areas. Then, a full-frame mapping camera is used to conduct ultra-high-resolution drone photogrammetry, combined with an instance segmentation model to complete juvenile contour extraction, coordinate positioning, density statistics, and age determination. This invention overcomes the shortcomings of traditional foot surveys, such as tidal limitations, small coverage area, susceptibility to environmental interference, high missed detection rate, and high personnel risk. It achieves non-contact, high-efficiency, high-precision, and low-disturbance juvenile horseshoe crab surveys, providing a scientific and efficient technical solution for the monitoring of juvenile horseshoe crabs in China and the protection of endangered species.

The present disclosure relates generally to compositions, dosage forms, and methods for the prevention and treatment of cancer and infections. One example method entails administering to a patient an effective amount of a composition comprising 1 * 107 to 500 * 107 intact, stable and substantially inactive Escherichia coli cells that have been treated to result in an untreated Escherichia coli cell, when measured by a tachypleus amebocyte lysate (LAL) assay, as compared to an untreated wild-type Escherichia coli cell. Lipopolysaccharide (LPS)-related endotoxin activity is reduced by about 70% to 99%, and wherein the composition contains LPS of 124 to 62000 endotoxin units (EU).

The invention discloses a cysteine hydrochloride bacterial endotoxin detection method, and relates to the technical field of bacterial endotoxin detection.The cysteine hydrochloride bacterial endotoxin detection method comprises the following steps that a test solution is prepared, specifically, a sodium hydroxide solution and bacterial endotoxin detection water are added into cysteine hydrochloride, and a test stock solution is prepared; then diluting with bacterial endotoxin inspection water to prepare a test solution; adding a sample and reacting, namely mixing the test solution with the tachypleus amebocyte lysate in equal volume, putting the mixture into a reaction tube, and calculating the content of endotoxin by using an endotoxin gel method tester. Experiments such as a tachypleus amebocyte lysate sensitivity recheck test, an interference test and a test sample preparation standard recovery test (interference test) prove that the test sample solution prepared by the method can be used for detecting bacterial endotoxin of tachypleus amebocyte lysate with sensitivity of 0.06 EU / ml, and the detection method is scientific, simple, convenient and reasonable, can eliminate the interference effect of a sample, and can be used for detecting the bacterial endotoxin of the tachypleus amebocyte lysate with the sensitivity of 0.06 EU / ml. And the accuracy of the inspection result is improved.

The invention discloses a novel endotoxin sensing detection device and method based on tachypleus amebocyte lysate, and relates to the field of endotoxin inspection.The device comprises a light source, a micro-nano fiber bragg grating sensor, a capillary tube, a reflected signal detection unit and a processing unit, and the light source inputs light signals into the micro-nano fiber bragg grating sensor; after the capillary tube extracts a to-be-detected sample solution, the to-be-detected sample solution is in contact with the micro-nano fiber bragg grating sensor; the turbidity of the to-be-detected sample solution is changed due to the reaction of endotoxin in the to-be-detected sample solution and the tachypleus amebocyte lysate, so that the resonant wavelength of the micro-nano fiber bragg grating sensor is changed, and the reflected signal detection unit receives a reflected light signal of the micro-nano fiber bragg grating sensor; the processing unit is used for detecting the concentration of endotoxin in the sample solution to be detected according to the resonant wavelength in the reflected light signal. According to the application, high-sensitivity quantitative detection of endotoxin can be realized under the condition of a small amount of tachypleus amebocyte lysate.

The invention discloses an edaravone raw material bacterial endotoxin inspection method, and belongs to the technical field of medicine quality control. According to the method, a gel method tachypleus amebocyte lysate is taken as a core, and the interference of an edaravone molecular pyrazolone group and a residual intermediate on detection is effectively eliminated by optimizing the dilution concentration of a test sample, establishing a gradient dilution interference test system, treating a turbid sample by combining a 0.22 mu m pyrogen-free filter membrane, retesting double-batch reagent verification and other key technologies. Triple quality control is set to ensure reaction effectiveness, complex instrument analysis is not needed, whether bacterial endotoxin is qualified or not can be accurately judged, the blank of exclusive detection of edaravone raw material endotoxin is filled, and the method is suitable for industrial quality control.