3 results about "Tachypleus" patented technology

The invention discloses a reaction buffer solution for improving endotoxin detection sensitivity of a recombinant tachypleus amebocyte lysate, a kit containing the reaction buffer solution and application of the reaction buffer solution. The reaction buffer solution comprises the following components: a Tris-HCl buffer solution, magnesium ions, sodium chloride, cane sugar, ethylene glycol diethyl ether diamine tetraacetic acid, a nonionic surfactant and cyclodextrin or a derivative thereof. The invention also discloses a preparation method of the reaction buffer solution and a kit containing the reaction buffer solution. When the reaction buffer solution or the kit is used for detecting the endotoxin of the recombinant tachypleus amebocyte lysate, the sensitivity of the recombinant tachypleus amebocyte lysate for detecting the endotoxin can be improved by one order of magnitude, the lowest detection limit can reach 0.0005 EU / ml, the reaction buffer solution or the kit has strong anti-interference capability and short reaction time, and the detection efficiency is greatly improved.

This invention discloses a method for surveying juvenile horseshoe crabs in China based on remote sensing technology and intelligent image recognition. Through a process of rapid comprehensive survey and precise regional survey, a drone equipped with a lightweight YOLO recognition model, RTK positioning, and laser ranging is used to conduct a comprehensive survey of potential habitats, identify juvenile distribution hotspots, and delineate precise survey areas. Then, a full-frame mapping camera is used to conduct ultra-high-resolution drone photogrammetry, combined with an instance segmentation model to complete juvenile contour extraction, coordinate positioning, density statistics, and age determination. This invention overcomes the shortcomings of traditional foot surveys, such as tidal limitations, small coverage area, susceptibility to environmental interference, high missed detection rate, and high personnel risk. It achieves non-contact, high-efficiency, high-precision, and low-disturbance juvenile horseshoe crab surveys, providing a scientific and efficient technical solution for the monitoring of juvenile horseshoe crabs in China and the protection of endangered species.

The invention discloses an edaravone raw material bacterial endotoxin inspection method, and belongs to the technical field of medicine quality control. According to the method, a gel method tachypleus amebocyte lysate is taken as a core, and the interference of an edaravone molecular pyrazolone group and a residual intermediate on detection is effectively eliminated by optimizing the dilution concentration of a test sample, establishing a gradient dilution interference test system, treating a turbid sample by combining a 0.22 mu m pyrogen-free filter membrane, retesting double-batch reagent verification and other key technologies. Triple quality control is set to ensure reaction effectiveness, complex instrument analysis is not needed, whether bacterial endotoxin is qualified or not can be accurately judged, the blank of exclusive detection of edaravone raw material endotoxin is filled, and the method is suitable for industrial quality control.