Anti-interference alkali reagent and preparation method and application method thereof

Abstract

The invention relates to an anti-interference alkali reagent. The anti-interference alkali reagent comprises the following components: potassium hydroxide, sodium chloride, polyprene, bis-glycinate, an ethyleneimine polymer, calcium chloride, magnesium chloride and triton X-100; uniformity and stability of serum can be enhanced by the above components, a lot of protease, blood coagulation factors,and globulin in serum can be simultaneously removed, the protease interference tachypleus amebocyte lysate tests can be greatly reduced, the anti-interference capability is greatly increased, and theideal recovery rate is obtained; an enzyme reaction of a tachypleus amebocyte lysate can be activated at a maximum degree by calcium chloride and magnesium chloride in the formula components, and thereaction capability of endotoxin and the tachypleus amebocyte lysate can be greatly increased; in addition, after usage of the alkali reagent, a Tris-HCl buffer solution is added, the detected serumhas uniformity and stability, is subjected to a cross reaction with endotoxin, an endotoxin structure is protected with a maximum degree, and the endotoxin concentration can be accurately detected.

Description

technical field [0001] The invention relates to the field of biomedical limulus reagent detection, in particular to an anti-interference alkali reagent and its preparation and use methods. Background technique [0002] Limulus reagent detection kit has been used clinically for many years and can be used to detect endotoxin in human serum. Because there are many proteases, blood coagulation factors, globulin in human serum. The LAL itself is also a process of serine protease hydrolysis reaction, that is, the LAL reagent activates the protease in the LAL reagent due to the action of endotoxin to cause them to produce a gradual hydrolysis reaction. These proteases in human blood can interfere with the LAL test. At present, most hospitals use the heating dilution method to process serum samples before the detection of LAL reagents. The heating dilution method is difficult to completely remove interfering substances in serum (there are many proteases, coagulation factors, globu...

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Problems solved by technology

However, the KOH alkaline method only contains potassium hydroxide and sodium chloride, which cannot completely remove interfering substances in the blood. The recovery rate of this method is in the range of 50-60%, which will also affect the accuracy of the detection, resulting in false negative results.

In addition, after serum was treated with KOH alkali method, some residual components inhibited the enzymatic reaction between endotoxin and Limulus reagent, which also caused the test result to be false negative.

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