74 results about "Ethyleneimine" patented technology

A polymer comprising recurring units represented by formula (I):

• • wherein X is selected from the group consisting of C(O)OR¹, C(O)SR¹, C(O)NR¹R², and VZ, where R¹ and R² are each individually selected from the group consisting of hydrogen, C₁ to C₁₀ alkyl, and C₆ to C₁₀ aryl, where V is a labile linker group, and where Z is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, and hexadecaamine dendrimer; and wherein Y is selected from the group consisting of —(CH₂)₂—, —(CH₂)₂—O—(CH₂)₂—, —(CH₂)₂—O—(CH₂)₂—, and —(CH₂)₃—NHC(O)—(CH₂)₆—C(O)NH—(CH₂)₃— is useful in nucleic acid delivery applications. Polyacetals of the formula (I) are preferably made by reacting appropriate diols and divinyl ethers. In preferred embodiments, complexes formed between polyacetals of the formula (I) and polynucleotides are useful as transfection reagents.

A polymer-carbon sorbent for removing carbon dioxide, heavy metals and toxic materials from a flue gas from a combustion process, such as coal-fired power plants, is described. The sorbent comprises a carbonaceous sorbent material and a cured amine-containing polymer, and sulfur. The polymer-carbon sorbents are formed by curing a curable amine-containing polymer in the presence of the carbonaceous sorbent material, sulfur, a cure accelerator and, optionally, a cure activator. A convenient carbonaceous sorbent material is an activated carbon, and a convenient curable amine-containing polymer is an allyl-containing poly(ethyleneimine), having a number average molecular weight between about 1,000 and about 10,000. The polymer-carbon sorbents may contain sulfur in excess of an amount needed to cure the curable amine-containing polymer. Such polymer-carbon sorbents are shown to capture more mercury, in both elemental an ionic forms, compared to activated carbon and adsorb carbon dioxide.

A method of synthesizing a random copolymer of polyethyleneimine and polyethylene glycol, comprising exposing ethanolamine in a solution to electromagnetic radiation for a sufficient time to polymerize the ethanolamine (OHCH₂CH₂NH₂) and thereby resulting in formation of the randome copolymer comprising polyethyleneimine and poly(ethylene glycol), wherein the polyethyleneimine comprises ethyleneimine (—CH₂CH₂NH—) unit and the polyethylene glycol comprises ethylene glycol (—CH₂CH₂O—) unit, and the polyethylenimine of the random copolymer has a substantially linear backbone.

The invention relates to a water-soluble carbon nano-tube and a preparation method and application method of the water-soluble carbon nano-tube, and solves the problem that the carbon nano-tube has a highly hydrophobic surface so as not to dissolve into water and organic solvents. The technical scheme is as follows: the carbon nano-tube is connected with a water-soluble polymer-polyethyleneimine by chemical bonds, and the weight ratio of the carbon nano-tube to the polyethyleneimine is 1:(0.085-0.125). The preparation method of the water-soluble carbon nano-tube comprises the following steps of: treating the carbon nano-tube by acid to obtain the carbon nano-tube with carboxyl groups on the surface; reacting the treated carbon nano-tube with ammoniation agent to obtain the carbon nano-tube with amino groups on the surface; reacting the carbon nano-tube with amino groups with ethyleneimine and making ethyleneimine continuously graft on amino groups in the presence of hydrogen ions; and finally, forming polyethyleneimine carbon nano-tube, i.e., the water-soluble carbon nano-tube. The water-soluble carbon nano-tube prepared by the method provided by the invention does not damage the structure of the carbon nano-tube, and has the characteristics of strong water dispersing property, low toxicity, good physical and chemical stability, low need in the preparation conditions, abundant raw materials and low cost.

To provide a primer composition for assisting an acrylic adhesive which exhibits a temporarily fixing effect very quickly and which is completely cured quickly at room temperature, while maintaining excellent characteristics of the acrylic adhesive.

A primer composition, which comprises a component (A): a basic compound having a primary, secondary or tertiary amine structure, a component (B): a liquid oligomer, and a component (C): a solvent. Preferably, the component (A) is a compound having a main chain of which the skeleton is ethyleneimine, or ethyleneimine having a molecular weight of from 50 to 70,000. Further, a bonding method, which comprises applying the above primer composition on a surface of an object to be bonded by dot coating, and bonding the object to another object to be bonded by means of the acrylic adhesive.

The invention discloses a preparation method of a light-responsive PEGylated gene delivery system based on host-guest assembly. Its steps are as follows: 1) Using N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, prepare polyethyleneimine grafted cyclodextrin solution with a concentration of 0.1-1 mg/mL and a concentration of 0.4-2 mg/mL azobenzene-modified polyethylene glycol solution; 2) Use the buffer solution in step 1) to prepare a DNA solution with a concentration of 50-500 μg/mL; 3) Mix the two solutions in step 1) and polymerize The molar ratio of cyclodextrin and azobenzene-modified polyethylene glycol contained in ethyleneimine-grafted cyclodextrin is 1:1-1:4, and the supramolecular compound solution is obtained after ultrasonication for 15 minutes and standing for 1 hour; 4) Add the solution prepared in step 3) to an equal volume of the solution in step 2), vortex to mix and let stand. The invention can improve its stability in physiological saline solution. Through the photoisomerization of azobenzene, the detachment of the PEG shell of the intracellular gene delivery system can be achieved, which can effectively improve the efficiency of gene transfection.

The present invention provides an aqueous solution comprising an antibody protein at a concentration of at least about 10 mg/mL and an oligomer of ethyleneimine, wherein the number of repeating units of ethyleneimine (n) in the oligomer is in the range n=2-12.

The present invention concerns a water-processable n-type semiconductor comprised of polyvinylpyrrolidone (PVP), carbon nanotubes (CNTs) and poly(ethyleneimine) (PEI). The semiconductors are prepared by providing PVP and CNTs in a hydrophilic slurry and dispersing therein small amounts of PEI.

The invention discloses a differential culture medium applied to carnation anther culture and belongs to the technical field of flower culture. The culture medium is prepared from components includingKNO3, NH4NO3, H2PO4, K2HPO4, MgSo4.7H2O, Ca(NO3)2, Fe-Gly, KI, H3BO3, MnSO4.4H20, ZnSo4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, vitamin B1, vitamin B6, niacin, folic acid, vitamin E, rutin, nucleotide, butyl hydroxy anisd, NaHS, ethyleneimine, AOAA, PHE, a pea cooling path, beta-glucan, inositol, 2-IP, NAA, KT, cane sugar and plant gel. The differential culture medium is used for differentialculture of carnation anther calluses, a highest average green plantlet differentiation rate reaches up 40.94%, and is of important significance to establishment of a complete carnation anther culturesystem.

The invention relates to a method for extracting and separating polypeptides. According to the method, large-size silicon dioxide (submillimeter scale) covalent loaded polyethyleneimine is adopted as an adsorbent (SiO2@PEI), polypeptides of different isoelectric points can be extracted in different pH environments, and the polypeptides can be efficiently released if the pH values are reduced. The reason is that the adsorbent is easy to protonize to be of positive ion, and the polypeptides can carry positive electricity instead of negative electricity along with reduction of the pH values, so that the polypeptides can be released as static complementary adsorption is changed into static mutual exclusion with the adsorbent. The complexing constant of the adsorbent and the polypeptides is generally greater than 10<12>M<-1>, so that even trace polypeptides with the sub-femtomolar content can be extracted. In addition, the release rate is 79-92% for most polypeptides. By adopting the adsorbent, at least polypeptides of which the relative abundance is only 0.1% can be extracted. The adsorbent is simple to prepare and can be used repeatedly.

The invention discloses a synthesis method of ethyl N-cyanoethanimideate, and the method comprises the following steps: adding ethyl ethyleneimine hydrochloride into a reaction kettle with stirring, adding a cyanamide aqueous solution under stirring, and stirring until the ethyl ethyleneimine hydrochloride is fully dissolved; dropping an alkaline substance to adjust pH value; allowing the materials to be fully reacted under normal temperature and stirring state, taking samples every 10 min in the reaction process, and detecting the samples by chromatography to determine the conversion rate of the reaction; standing after an aqueous phase and an organic phase in the reactant are layered, extracting the organic phase out and then distilling organic phase the under reduced pressure to obtain an ethyl N-cyanoethanimideate product. The synthesis method is simple and feasible, energy-saving and environment-friendly, solves the problems of complex reaction, pollution and the like in the synthesis process, and saves the cost at the same time.

The invention relates to a one-step synthesis method of polyethyleneimine. The method comprises the following steps: (1) synthesizing monomer ethyleneimine, reacting ethanolamine with concentrated sulfuric acid or hydrogen chloride, and treating with strong base to obtain monomer ethyleneimine and an inorganic salt byproduct; and (2) synthesizing polyethyleneimine, namely directly polymerizing the monomer ethyleneimine obtained in the step (1) under the conditions of an acid catalyst and a polyhaloalkane catalyst without separation treatment to generate polyethyleneimine. According to the one-step synthesis method, on one hand, the use and storage risk and harm caused by high reactivity, corrosivity, toxicity, flammability and explosiveness of the ethyleneimine are avoided; on the other hand, special treatment for removing residual ethyleneimine in the monomer separation process is also avoided. According to the method disclosed by the invention, an intermediate separation step is not needed, so that the possible risks of poisoning, combustion and explosion to operators are greatly reduced, and the pressure of environmental protection is also greatly reduced.

The invention relates to a metal ion extractant material for use in the extraction of metal ions from a solution or slurry, the extractant material being based on an ethyleneimine-containing polymer. The ethyleneimine-containing polymer may be attached to a solid substrate which may optionally be ground to form a particulate extractant material. Alternatively, an ethyleneimine-containing polymeric material may be cross-linked and subsequently ground to form a particulate metal ion extractant material without the need for a solid substrate.

By adopting methods of fluorescent quantitation PCR, fluorescent quantitation RT-PCR, HBV DNA quantitation, HBsAg quantitation, HBeAg quantitation, cccDNA quantitation, Northern blot, Southern blot, Western blot, immunohistochemistry, and the like in the research and using the maintenance dose within the treating dose range for a long time (30-60 days), supernatant HBV DNA HBsAg HBeAg cultured by HepG-2.2.15 cells can completely disappear, HBsAg, HBeAg, HBcAg, and the like in the cells are completely turned to be negative, HBV DNA is in a high inhibited state, HBV cccDNA is completely negative, fluorescent quantitation RT-PCR detection finds that the mRNA for expressing HBsAg and HBcAg antigens in the cells is completely negative, and in addition, the curative effect of the atabrine is 30 times that of lamivudine as a first-line drug for resisting HBV in current clinic. In order to illustrate the action mechanism of the atabrine and the pyronaridine as a drug belonging to the same kind with the atabrine, an HBV genome is divided into 3 segments which are respectively inserted into an Xb1 position on a luciferase report carrier PGL3, a multifunctional microplate reader detects and finds that the light production value of the luciferase is remarkably reduced, which shows that the molecules of the atabrine and the pyronaridine as a drug belonging to the same kind with the atabrine can be nonspecifically combined with the HBV DNA in the cells, thereby inhibiting the copying of the virus and ensuring that the HBV DNA content of the virus in cells copied by the filial generation is reduced till to disappear. The patent requires protecting the application of 3 linked benzyl structures (named as ethyleneimine) and radicals such as CH3O-,-NH-, CL-, and the like for resisting HBV virus in clinic.

The invention relates to an additive for biomass fuel. The additive is prepared by mixing the following raw materials of benzotriazole, methanol, ethylene glycol monoethyl ether 5, ethylhexyl ester, ethylene glycol, ethyleneimine, monoethanolamine, triethanolamine, isopropyl alcohol, ammonia water, and cyclohexylamine carbonate; firstly, adding the benzotriazole into the methanol, stirring and dissolving, adding the cyclohexylamine carbonate, fully dissolving, adding the ethylene glycol monoethyl ether, and finally adding the ethylene glycol, so as to obtain a solution A; sequentially adding the triethanolamine, the monoethanolamine and the ethyleneimine according to the ratio at the interval of 10min; while the raw materials are added, continuously stirring is performed, so as to obtain a solution B; respectively adding the solution A and the solution B into two ammonia water solutions, dissolving, mixing the two solutions, and stirring for 10min, so as to obtain a solution C; adding the ethylene glycol into the solution C, and uniformly stirring, so as to obtain the additive. The additive has the advantages that the stability is good, the additive is suitable for being popularized, the use effect is obvious, the combustion is complete, the heat efficiency is improved, and the oil consumption is decreased.