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Differential culture medium applied to carnation anther culture

A differentiation medium, carnation technology, applied in the direction of application, horticulture, botanical equipment and methods, etc., can solve the problem of not obtaining haploid or DH plants

Inactive Publication Date: 2020-08-14
陈滋倩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reports, Mutty et al. (1976) and Villalobos (1981) carried out anther culture on Carnation, but did not obtain haploid or DH plants; Mosquera et al. (1999) carried out anther culture on Carnation cultivar 'Tanga' , 30 embryoid bodies were obtained, but only 1 regenerated plant was obtained

Method used

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  • Differential culture medium applied to carnation anther culture
  • Differential culture medium applied to carnation anther culture
  • Differential culture medium applied to carnation anther culture

Examples

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Effect test

Embodiment 1

[0017] 1 Materials and methods

[0018] 1.1 Test materials

[0019] The callus induced by carnation cultivars 'Mast' and 'free' anther culture was obtained by the inventor's previous experiment.

[0020] 1.2 Test method

[0021] Transfer the callus induced by carnation anther culture to the differentiation medium, and place it under the conditions of temperature 25±2℃, humidity 50%~70%, light time 14~16h / d, and light intensity 1500~2000lx Cultivate, observe once every 5 days, record the differentiation of callus, and count the green shoot differentiation rate of callus after 30 days of culture.

[0022] Differentiation rate of green shoots (%) = number of callus blocks that differentiated into green shoots / total number of callus blocks × 100

[0023] The composition and concentration of the differentiation medium are as follows: KNO 3 1000mg / L, NH 4 NO 3 800mg / L, KH 2 PO 4 95mg / L, K 2 HPO 4 55mg / L, MgSO 4 ·7H 2 O 210mg / L, Ca(NO 3 ) 2 340mg / L, iron glycinate 29....

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Abstract

The invention discloses a differential culture medium applied to carnation anther culture and belongs to the technical field of flower culture. The culture medium is prepared from components includingKNO3, NH4NO3, H2PO4, K2HPO4, MgSo4.7H2O, Ca(NO3)2, Fe-Gly, KI, H3BO3, MnSO4.4H20, ZnSo4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, vitamin B1, vitamin B6, niacin, folic acid, vitamin E, rutin, nucleotide, butyl hydroxy anisd, NaHS, ethyleneimine, AOAA, PHE, a pea cooling path, beta-glucan, inositol, 2-IP, NAA, KT, cane sugar and plant gel. The differential culture medium is used for differentialculture of carnation anther calluses, a highest average green plantlet differentiation rate reaches up 40.94%, and is of important significance to establishment of a complete carnation anther culturesystem.

Description

technical field [0001] The invention relates to a differentiation medium for carnation anther culture, belonging to the technical field of flower cultivation. Background technique [0002] Carnation ( Dianthus caryophyllus L . ) is a perennial root flower of the family Caryophyllaceae, also known as carnation, lion head carnation, musk carnation, large flower carnation, Dutch carnation, evergreen subshrub, native to southern Europe, the Mediterranean coast to India, and has been in Europe for more than 2,000 years It has a history of cultivation and is a famous flower in the West. At present, carnation is mainly produced in Italy, Spain, the Netherlands, Poland, the United States, Turkey, Colombia and Israel. Carnation is rich in flower colors. The original flower is deep pink. After long-term cultivation, the flower diameter increases and the double petals increase. The flower colors also include white, pink, rose red, bright red, deep red to purple, milky yellow to yell...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 陈滋倩
Owner 陈滋倩
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