3614 results about "Glucan" patented technology

A process for enzymatic preparation of poly (α1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to fructose and poly (α1, 3 glucan). Application of semi-permeable membranes to continuously remove fructose, a by-product of the gtf enzyme, thus increasing the poly (α1, 3 glucan) liter, is disclosed.

A process for the production of beta-3-(1,3)(1,6) glucan from a glucan containing cellular source is described, together with compositions and uses / methods of treatment involving glucan. The process of the invention comprises the steps of: (a) extracting glucan containing cells with alkali and heat, in order to remove alkali soluble components; (b) acid extracting the cells of step (a) with an acid and heat to form a suspension; (c) extracting the suspension obtained of step (b) or recovered hydrolyzed cells with an organic solvent which is non-miscible with water and which has a density greater than that of water separating the resultant aqueous phase, solvent containing phase and interface so that substantially only the aqueous phase comprising beta-(1,3)(1,6) glucan particulate material remains; wherein the extraction with said organic solvent provides separation of glucan subgroups comprising branched beta-(1,3)(1,6)-glucan, and essentially unbranched beta-(1,3) glucan which is associated with residual non-glucan contaminents; and (d) drying the glucan material from step (c) to give microparticulate glucan.

Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.

A process for enzymatic preparation of poly (α1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to fructose and poly (α1, 3 glucan). Application of semi-permeable membranes to continuously remove fructose, a by-product of the gtf enzyme, thus increasing the poly (α1, 3 glucan) liter, is disclosed.

A compound which can specifically or selectively expresses an antifungal activity with a broad spectrum, based on the functional mechanism of 1,6-β-glucan synthesis inhibition, is provided, and an antifungal agent which comprises such a compound, a salt thereof or a solvate thereof is provided. A compound represented by the following formula (I), a salt thereof or a solvate thereof.

A process for enzymatic preparation of a highly linear poly(α1,3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to a highly linear poly(α1,3 glucan) in high titers. Hydrolyzed poly(α1,3 glucan) is used as the primer for the gtfJ enzyme reaction resulting in the formation of highly linear poly(α1,3 glucan).

The invention relates to a method for controlling plant diseases caused by the fungi Botrytis cinerea and Alternaria alternata, by applying to a growing plant or to fruit or vegetables before or after harvesting, a composition which comprises an effective amount for controlling said fungi of at least one oligosaccharide ingredient, active against Botrytis cinerea and Alternaria alternata, and selected from oligosaccharides obtainable by hydrolysis of chitin, beta -glucan and other similarly active polysaccharides, excluding chitosan, of cell walls of fungi, yeasts, marine plants and exoskeletons of arthropods, The composition also forms part of the invention, as does an analogous method and composition for a method for controlling plant diseases caused by Botrytis cinerea, and utilizing at least one oligosaccharide ingredient, active against Botrytis cinerea selected from oligosaccharides obtainable by hydrolysis of chitosan, and having a molecular weight within the range of about 500 to about 10,000 daltons, provided that in this instance the composition excludes acetic acid.

Compositions comprising cellulase and at least one poly alpha-1,3-glucan ether compound having a degree of substitution with an organic group of about 0.05-3.0 are disclosed. Such compositions can be dry or aqueous, the latter of which can have a viscosity of at least about 10 cPs. The disclosed composition can be in the form of a personal care product, household product, or industrial product, for example. Also disclosed are a method for preparing an aqueous composition comprising cellulase and a poly alpha-1,3-glucan ether compound, and a method of treating a material such as fabric by contacting it with this aqueous composition.

The invention discloses a composite capable of promoting skin micro-ecological balance and a preparation method of the composite. The composite comprises the following main ingredients of skin prebiotics alpha-glucan oligosaccharide, fructooligosaccharide, beta-glucan, plant extract, yeast extract and natural antibacterial agent leuconostoc / radish root fermentation product filter liquor. According to the composite, micro-ecological balance of the skin can be fundamentally adjusted, skin health is kept, the defects that traditional cosmetics or skin care products cure symptoms, not diseases and are prone to generating side effects are overcome, and the composite has the moisture preservation, softening and tightening multi repair effects, and provides a new thought for solving the skin problems. The preparation method of the composite is simple and practicable, and active ingredients of the functional components are kept to the maximum degree by reasonably controlling reaction steps and mixing temperature.

The present invention relates to methods of using neutral soluble glucan and monoclonal antibodies for antitumor therapy. Neutral soluble Beta (1,3; 1,6) glucan (NSG) enhances the tumoricidal activity of the innate immune system by binding to the C3 complement protein receptor CR3. The glucan does not stimulate the induction of inflammatory cytokines. Also described are methods of using whole glucan particles (WGP) as an immunomodulator by inducing a shift from a Th2 response to the Th1 response, leading to an enhanced antitumor cytotoxic T-cell response.

The invention features a food product containing a probiotic and β-glucan isolated from a natural source, methods of treating a disease or disorder by administering the food product, and a package containing separated components of the food product.

Isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups using isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

A β-glucan-containing fat and oil composition contains β-glucan of microorganism origin or basidiomycete origin. The β-glucan-containing fat and oil composition has β-glucan uniformly dispersed in a food without worsening the texture, taste etc. of the food. The novel microorganism can efficiently produce β-glucan which has a high activity and favorable qualities as β-glucan of microorganism origin as described above from less expensive saccharides such as sucrose at a high production speed.

Particulate β-glucan is solubilized at elevated pressure and temperature to form soluble β-glucan. The method is safe and economical and produces a product that is an improved pharmaceutical agent.

The invention provides methods and compositions for the detection and / or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.

By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.

A genetically modified organism comprising: at least one nucleic acid sequence and / or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups; and at least one nucleic acid sequence and / or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and / or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and / or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and / or xylan-, glucan-, galactan, or mannan-decorating groups.

The invention discloses a germicidal antiviral composite containing chlorine dioxide. The composite comprises a functional composition that can provide chlorine dioxide and a stabilizing agent, wherein the stabilizing agent is a compound that can cause the functional composition capable of providing the chlorine dioxide to stably exist in a solvent or a carrier and comprises one or more of EDTA, carboxymethyl cellulose, sodium laurate, polyethylene glycol, cyclodextrin, glucan, chitin, and mucopolysaccharide and the like. The technical means adopted in the invention solves the difficulty thatthe chlorine dioxide is instable in products or a complicated stabilizing and releasing technical means is needed in application of products, thus expanding the production and application range of products.

The invention discloses an anti-wrinkle and anti-aging cosmetic and a preparation method thereof, and belongs to the technical field of cosmetics. The anti-wrinkle and anti-aging cosmetic contains the following raw materials: 1%-3% of an emulsifier, 5%-15% of a humectant, 5%-15% of an emollient, 2%-6% of active matters and the balance of water, wherein the active matters are trifluoroacetyl tripeptide-2 and a skincare composition of glycerinum and glucosan, an echinacea extract and a white truffle extract; the skincare composition has a synergistic effect in improvement of the skin elasticity. The cosmetic disclosed by the invention is capable of increasing the skin elasticity, reducing fine lines and wrinkles of facial skin and relieving skin dropping and sagging and wrinkles, and simultaneously has the characteristics of being light in texture and easy to absorb.

A process for the production of beta-(1,3)(1,6) glucan from a glucan containing cellular source is described, together with compositions and uses / methods of treatment involving glucan. The process of the invention comprises the steps of: (a) extracting glucan containing cells with alkali and heat, in order to remove alkali soluble components; (b) acid extracting the cells of step (a) with an acid and heat to form a suspension; (c) extracting the suspension obtained of step (b) or recovered hydrolyzed cells with an organic solvent which is non-miscible with water and which has a density greater than that of water separating the resultant aqueous phase, solvent containing phase and interface so that substantially only the aqueous phase comprising beta-(1,3)(1,6) glucan particulate material remains; wherein the extraction with said organic solvent provides separation of glucan subgroups comprising branched beta-(1,3)(1,6)-glucan, and essentially unbranched beta-(1,3) glucan which is associated with residual non-glucan contaminents; and (d) drying the glucan material from step (c) to give microparticulate glucan.

The invention discloses a radiation-resistant and moisture-retention sun-blocking skin-care product, which contains 2-5 wt% of oat beta-glucan. The radiation-resistant and moisture-retention sun-blocking skin-care product provided by the invention can be used to raise radiation resistance and immunity of the whole skin and construct healthy skin, has an anti-allergic effect, can effectively prevent / alleviate various inflammation and symptoms such as erythema, and can preserve moisture, minimize fine lines and smoothen skin.

This invention pertains to a novel process for preparing fibers from poly(α(1→3) glucan). The fibers prepared according to the invention, have“cotton-like” properties, are useful in textile applications, and can be produced as continuous filaments on a year-round basis. The process comprises solution spinning from a novel solution of poly(α(1→3) glucan) in a mixture of water and N-methylmorpholine-N-oxide followed by coagulation in a liquid coagulant that comprises a liquid that is not water.

Anti-glucan antibodies have been found to be protective against systemic fungal infection with C. albicans, but the protective efficacy can be inhibited by blocking antibodies. The invention provides an immunogenic composition comprising a glucan and a pharmaceutically acceptable carrier, characterised in that, when administered to a mammalian recipient, the composition elicits protective anti-glucan antibodies but does not elicit antibodies which inhibit the protective efficacy of the anti-glucan anti-bodies. The glucan may be presented on the surface of a protease-treated microbial cell or may be presented as a protein-glucan conjugate. The glucan may be substituted by a glucan mimotope, a peptidomimetic of a glucan mimotope, or nucleic acid encoding a mimotope. Anti-glucan-antibodies show broad spectrum microbicidal activity. B-glucans are preferred, particularly those containing one or more B-1,6 linkages

The invention relates to the use of water soluble β-(1,3) glucans, which are essentially free from (1,6) links as active ingredients agents for producing therapeutic skin treatment agents. Said products strengthen the skin's immune system, counteract the formation of wrinkles and can be used to treat psoriasis and eczemic conditions.

The invention relates to a method for preparing barely bran or oat bran, which is used to produce edible fiber foodstuff, and utilization. The invention enables maximum release of soluble edible fiber especially beta-glucose in barely or oat, increases its solubility in water from 4-55 to 6-9%, and the edible fiber in barely or oat is as high as above 25%. The product is characterized by improved bran fiber taste, improved health-care function for barely or oat, greatly increased grade and quality of barley or oat, and promoted quality for traditional foodstuff. The product can supply edible fiber directly, effectively, safely and conveniently, which makes our blood and bowel be clean and healthy, and is effective for following chronic disease and : diabetes, high blood fat, high cholesterin, fat, functional constipation, fatty liver and oronary heart disease.

Solutions formed by combining poly(α(1→3) glucan) with CS2 in aqueous alkali metal hydroxide solution have been shown to produce the xanthated form of the poly(+(1→3) glucan). The solutions so formed have been shown to be useful for solution spinning into fiber of poly(α(1→3) glucan) when the spun fiber is coagulated in an acidic coagulation bath. The fibers so produced exhibit desirable physical properties. The poly(α(1→3) glucan) employed was synthesized by fermentation.

The invention relates to a general-purpose high-efficiency bulking compound feed for a culter alburnus and a pelteobagrus fulvidraco. The compound feed is prepared from the following raw materials in portions by weight: 13-35 portions of imported steam fish meal, 10-25 portions of imported direct-heat fish meal, 3-5 portions of maggot powder, 2-8 portions of corn protein powder, 5-10 portions of soybean meal, 2-8 portions of fermented soybean, 2-4 portions of soybean meal, 3-8 portions of cottonseed protein, 2-5 portions of peanut meal, 2-5 portions of colza meal, 3-8 portions of meat / bone meal, 2-5 portions of sleeve-fish visceral meal, 23-33 portions of high protein flour, 1.0-3.0 portions of soya bean lecithin, 2.0-5.0 portions of sea fish oil, 0.4-1.0 portion of choline chloride (50%), 0.1-0.3 portion of vitamin C-phosphate (35%), 0.5-1.5 portions of calcium biphosphate, 0.2-0.5 portion of compound multi-vitamin, 0.5-1.5 portions of composite mineral salt, 0.04-0.2 portion of lutein (20%), 0.1-0.3 portion of phagostimulant, 0.1-0.3 portion of micro-ecological preparation, 0.1-0.3 portion of beta-1,3 glucan, 0.01-0.04 portion of phytase and 0.04-0.08 portion of non-starch polyase. The feed can meet all the nutritional requirements of culturing the culter alburnus and the pelteobagrus fulvidraco, so that fish bodies can grow quickly, high feed conversion rate can be achieved, and the coefficient of the feed ranges from 1.2 to 1.4. By using the feed, the immunity performance and the anti-stimulation capability of the culter alburnus and the pelteobagrus fulvidraco can be obviously enhanced, the energy of the fish bodies can be ensured, and the survival rate is improved by 5-10%.