33 results about "Streptomyces albidoflavus" patented technology

The invention discloses Bacillus siamensis B-3 and an application thereof. The collection number of the Bacillus siamensis B-3 at China General Microbiological Culture Collection Center is CGMCC No. 13753. Experiments prove that a strain obtained by culturing the Bacillus siamensis B-3 can inhibit growth of Streptomyces scabies, Streptomyces acidiscabies and / or Streptomyces albidoflavus causing potato scab, and the Bacillus siamensis B-3 and the related strain can be used for preventing and treating the potato scab.

The invention discloses a compound microbial agent with a function of effectively degrading cellulose at high temperature. The compound microbial agent contains five actinomycetes: Streptomyces thermoviolaceus, Streptomyces thermodiastaticus, Streptomyces thermocarboxydus, Streptomyces albidoflavus and Streptomyces thermovulgaris, and one bacteria: Brevibacillus borstelensis. The compound microbial agent solves the problem of slow composting and long cycle of garden waste due to the limitation in cellulose degradation difficulty, accelerates cellulose degradation in the composting process, shortens the fermentation period, and improves the conversion efficiency, thereby having a broad application prospect in the aspects such as the application of composting treatment and resource utilization of garden waste, and the like.

The invention discloses streptomyces albidoflavus Actin-1. The streptomyces albidoflavus Actin-1 can grow and propagate with apple tree rot pathogenic bacterium mycelium as nutrition, long-term colonization of biocontrol bacteria on apple tree rot scabs is promoted, a long-term biological prevention and treatment function is achieved, meanwhile, the streptomyces albidoflavus Actin-1 is induced to generate multiple ectoenzyme cell wall hydrolytic enzymes, and pathogenic bacterium cells disintegrate in combination with the enzyme dissolving function; high antibacterial active matter can be generated, the bacteriostasis rate on the apple rot pathogenic bacteria is 89.82%, a good antibacterial effect is achieved on botryosphaeria berengeriana and other pathogenic bacteria, the bacteriostasis rate ranges from 76.08% to 87.10%, and broad spectrum bacteriostasis performance is achieved. The streptomyces albidoflavus Actin-1 is adopted as main biocontrol bacteria for preventing and treating the apple tree rot and other fruit and vegetable pathogenic bacteria, and the advantages of being good in prevention and treatment effect (100%), high in efficiency, low in recurrence rate (0), high in environment adaption capacity, high in stability, not likely to generate resistance to drugs and the like are achieved. Important significance is achieved on improving the prevention and treatment effect on the apple tree rot and other fruit and vegetable pathogenic bacteria, preventing pathogenic bacterium relapse and protecting the environment.

The invention belongs to the field of microbes, and particularly provides a microbial composition for preventing and treating bacterial wilt of tobacco. The microbial composition is formed by combining three actinomyces bacteria of streptomyces microflavus CGMCC (China General Microbiological Culture Collection Center) 12841, streptomyces albidoflavus CGMCC 12842 and streptomyces pratensis CGMCC 12843 and one pseudomonas aeruginosa. The microbial composition can be used for effectively preventing and treating the bacterial wilt of the tobacco, is used for ameliorating the agronomic traits of the tobacco, adjusting the proportion of a beneficial microbe in soil and improving the yield of the tobacco and the proportion of superior cigarette. In addition, the invention also provides a culture method of the microbial composition.

The invention discloses an antibiological inoculant for controlling apple tree canker, and application of the antibiological inoculant. The antibiological inoculant can grow and propagate directly by using pathogenic mycelium as nutrition, and can induce the self to produce multiple extracellular enzyme capable of degrading cell walls of pathogenic bacteria; a streptomyces albidoflavus Actin-1 strain, colonized at disease spots of the apple tree canker for a long time, is activated and subjected to spore production, so that spore suspension is prepared; after that, a fermented seed solution is prepared by continuously carrying out liquid fermentation; finally, the fermented seed solution is inoculated into a solid fermentation medium, and is then fermented, dried in the air, crushed and screened so as to be prepared into spore raw powder; the spore raw powder is then scientifically screened and compounded by using auxiliary agents such as an ultraviolet protective agent, a dispersing agent and a carrier. The antibiological inoculant provided by the invention has the multiple advantages of being good in control effect, high in efficiency, low in recurrence rate and high in environmental adaptability and stability, not easy in producing drug-resistance, and the like, thus being a first choice for biological control; the antibiological inoculant has a great significance for improving the preventing and treating effect of fruit and vegetable pathogenic bacteria such as the apple tree canker, preventing pathogen recurrence and protecting environment.

The invention discloses a streptomyces albidoflavus W68 (with the strain collection number of CGMCC No. 12210) which has broad spectrum antifungal activity and is separated from deep sea sludge as well as an application technology of a microbial pesticide prepared from the strain and belongs to the technical field of microorganisms. The application technology of the microbial pesticide which is prepared from the streptomyces albidoflavus W68 separated from deep sea sludge and contains strain active spores and active enzymes and active secondary metabolites produced by the strain is simple, convenient, efficient and environment-friendly, and the concentration of spores of a solid fermented bacterium preparation can reach 10 billion. Pot experiments prove that the streptomyces albidoflavus W68 pesticide preparation separated from deep sea sludge shows remarkable control effects on soil-borne fungal diseases such as wheat root rot, melon fusarium wilt, phytophthora capsici and wheat take-all.

The invention discloses a composite microbial agent for a coal gangue artificial ecological matrix, which comprises five actinomycetes, streptomyces thermoviolaceus, streptomyces thermodiastaticus, streptomyces thermocarboxydus, streptomyces albidoflavus and streptomyces thermovulgaris, and one bacterium is pseudomonas sp. and one mould is penicillium oxalicum. The preparation process of the composite microbial agent comprises the following four steps: activating strains, preparing a seed solution, preparing a liquid composite microbial inoculum and preparing a solid microbial inoculum. According to the technology, the problems that microorganisms in the coal gangue are lacked, and nutrients are not beneficial to plant absorption and utilization are solved, a new way is provided for resource utilization of the coal gangue and landscaping waste, and the preparation has wide application prospects in the aspects of treatment, resource utilization and the like of the coal gangue.

The invention relates to an application of yingluo mycin in preparing drug resisting plant pathogenic fungi, wherein yingluo mycin is natural source antimycin antibiotics (Antimycin) and is obtained by separating Streptomyces albidoflavus I07A-01824 in fermentation liquor. Experiments prove that the compound has obvious function of resisting plant pathogenic fungi, can be further developed and researched into a high-efficient agricultural antibiotics and can be applied to the fields, such as crop cultivation, food refreshment and the like.

The invention discloses a nematicidal streptomyces albidoflavus (Streptomyces albidoflavus) and an application thereof in control of plant root-knot nematodes. The preservation number of the streptomyces albidoflavus is CGMCC No.12741. The streptomyces albidoflavus is separated from a soil sample. A fermentation liquid of a bacterial strain has obvious insecticidal activity on the plant root-knot nematodes and can be used for preparing a microbial agent for controlling the plant root-knot nematodes.

The invention discloses a high-temperature-resistant cellulose degrading bacterium strain Streptomyces albidoflavus of which the collection number is CGMCC No.12136. The strain can produce abundant enzymes at the high temperature of 40-70 DEG C, has the characteristics of high cellulase activity, high temperature resistance and high cellulose degradation efficiency, can be used as a microbial inoculant in a high-temperature composting system, and is suitable for garden waste composting.

The invention discloses bacillus atrophaeus and an application thereof. The bacillus atrophaeus provided by the invention is bacillus atrophaeus WS-1, and the preservation number of the bacillus atrophaeus WS-1 in the China General Microbiological Culture Collection Center is CGMCC No. 16198. Experiments prove that the bacillus atrophaeus WS-1 can inhibit streptomyces scabies and / or streptomyces acidiscabies and / or streptomyces albidoflavus, and has an important application value for preventing and treating potato scab. The invention has an important application value.

The invention relates to Streptomyces alboflavus, a method using the Streptomyces alboflavus to prepare a biocontrol microbial agent and application. The method includes: placing the Streptomyces alboflavus into a preservation culture medium, activating at 28-32 DEG C for 5-7 days, placing into a seed culture medium, and culturing at 30 DEG C and 160-180r / min for 36-48 hours to obtain a seed bacterium solution; inoculating the seed bacterium solution into a fermentation culture medium, performing fermentation culture in a constant-temperature culture tank of 30 DEG C for 7 days, placing the matrix obtained after the fermentation culture into a ventilation drying oven of 25-35 DEG C or an air energy heat pump drying machine for drying or subjecting the matrix to natural drying under normaltemperature, and crushing to obtain the biocontrol microbial agent. The prepared biocontrol microbial agent is applicable to ralatonia solanacearum antagonism, applicable to salt resistance, drought resistance and soil inorganic phosphorus dissolving, applicable to siderophore and growth hormone heteroauxin synthesis, and applicable to tomato seed germination promotion and growth promotion.

The invention discloses a preparation method of an apple tree canker antibiological inoculant. The method comprises the steps that apple tree canker pathogen hyphae can be directly adopted as nutrition for growth and breeding, apple tree canker pathogen hyphae can be induced to produce various exocellular enzymes for degrading pathogen cell walls, streptomyces albidoflavus Actin-1 colonized at apple tree canker scab for a long time is activated to produce spores, a spore suspension is prepared, then, the spore suspension is inoculated into a liquid seed culture medium to be fermented to obtain a fermentation seed solution, finally, the fermentation seed solution is inoculated into a solid fermentation culture medium for solid fermentation to obtain spore raw powder, and then, the spore raw powder, an ultraviolet protectant, a dispersing agent, a carrier and other auxiliaries are scientifically screened and compounded to obtain the antibiological inoculant with the multiple advantages of being good in control efficiency, high in efficiency, low in recurrence rate, high in environment adaptability, high in stability, not likely to generate drug resistance and the like; the antibiological inoculant is the first choice for biological control, and is of great significance in improving the control effect of garden stuff pathogenic bacteria such as apple tree canker, preventing pathogenic bacterium relapse and achieving environmental protection.

The invention discloses a strain of Streptomyces albidoflavus Z9 capable of preventing and controlling sunflower sclerotium blight at a seedling stage, and belongs to the technical field of microorganisms, wherein the strain has the preservation number of CGMCC 13115. According to the present invention, the results of the separation activation and identification of the pathogenic bacteria Sclerotinia scleroterum of sunflower sclerotium blight, the primary screening and secondary screening of antagonistic bacteria, the strain identification analysis of the antagonistic bacteria and other experiments show that the strain Z9 can strongly inhibit the hypha growth of Sclerotinia scleroterum; the peel infection incidence rate of the sunflower seed treated with the strain Z9 fermentation raw liquid is reduced by 70%, the seed germination promotion rate of the strain Z9 fermentation filtrate is 33.3%, the prevention and control effect of the strain Z9 fermentation raw liquid on the ex vivo leaf is 69.6%, the prevention and control effect of the strain Z9 fermentation raw liquid in potted plant experiments is 66.7%, and the strain Z9 can effectively inhibit the growth and reproduction of Sclerotinia scleroterum; and the strain has advantages of simple culture conditions, easy preservation, environment protection, and good development and application value.

The invention discloses a preparation method of anisin, a detection method of anisin and antibacterial application, the preparation method of anisin comprises the following steps: inoculating a strain seed solution of streptomyces albidoflavus CK-15 into a seed culture medium, culturing at 28 DEG C at 220rpm for 24h, transferring into 100ml of a fermentation medium according to an inoculation amount of 5%, fermenting at 30 DEG C for 5d, centrifuging the obtained fermentation liquid, collecting a first supernatant; adjusting the pH value of the first supernatant to 9.0, and extracting the first organic phase by using ethyl acetate with the same volume as the supernatant; carrying out reverse extraction on the first organic phase by using acid water with the same volume as the first organic phase and the pH value of 2.0, collecting a water phase and adjusting the pH value of the water phase to 9.0; extracting with ethyl acetate with the volume equal to that of water, collecting a second organic phase and spin-drying; according to the preparation method of the anisimycin, the streptomyces albidoflavus CK15 strain is fermented to prepare the anisimycin, and compared with a chemical synthesis method for preparing the anisimycin, the preparation method is simple, convenient to operate and low in cost.

The invention discloses a strain of Streptomyces albus W68 isolated from deep-sea sludge with broad-spectrum antifungal activity (the strain preservation number is CGMCC No. 12210) and the application technology of the microbial pesticide prepared by the fungus. It belongs to the technical field of microorganisms. The application technology of microbial pesticides prepared by Streptomyces microalbubus W68 isolated from deep-sea sludge, which contains both active spores of the strain and active enzymes and active secondary metabolites produced by the strain, is not only simple, efficient, but also environmentally friendly, and solid The spore concentration of the fermented bacterial preparation can reach tens of billions. Pot experiments have confirmed that the pesticide preparation of Streptomyces albicans W68 isolated from deep-sea sludge has shown obvious control effects on soil-borne fungal diseases such as wheat root rot, melon wilt, pepper phytophthora and wheat take-all .

The invention discloses a compound microbial bacterial agent used for coal gangue artificial ecological matrix, including 5 kinds of actinomycetes: Streptomyces violaceum ( Streptomyces thermoviolaceus ), Streptomyces thermophilus ( S.thermodiastaticus ), Streptomyces carbon monoxide ( S.thermocarboxydus ), Streptomyces albus ( S. albidoflavus ) and Streptomyces vulgaris ( S. thermovulgaris ), 1 bacteria: Pseudomonas ( Pseudomonas sp.), and 1 mold: Penicillium oxalicum ( Penicillium oxalicum ). The preparation process of the bacterial agent includes four steps: activation of the bacterial strain, preparation of the seed liquid, preparation of the liquid composite bacterial agent, and preparation of the solid bacterial agent. The technology of the invention solves the problem that the coal gangue lacks microorganisms and the nutrients are not conducive to plant absorption and utilization, and provides a new way for the resource utilization of the coal gangue and landscaping waste.

The invention belongs to the technical field of primer design, and discloses a primer group. The primer group is P2939123F and P2939374R and / or P1751437F and P1751612R. The primer group provided by the invention is high in specificity, high in sensitivity and high in detection efficiency, and can be used for effectively identifying streptomyces albidoflavus W68.

The invention discloses Serratia grimesii and application thereof. The Serratia grimesii is Serratia grimesii S-2, and the preservation number of the Serratia grimesii S-2 in the China General Microbiological Culture Collection Center is CGMCC No. 13034. Experiments prove that the Serratia grimesii S-2 can inhibit Streptomyces scabies and / or Streptomyces albidoflavus and has a significant application value on the preventing and treating of potato scab.

The invention discloses a vegetable fertilizer with gardenia jasminoides waste as a raw material and a preparation method thereof. The fertilizer comprises the following raw materials in parts by weight: 40-55 parts of gardenia jasminoides waste, 10-20 parts of citrus branches, 10-15 parts of waste straws, 6-12 parts of lignin, 5-9 parts of a complex microbial inoculant, 3-5 parts of a calcium fertilizer, 3-5 parts of a magnesium fertilizer, 3-5 parts of a potassium fertilizer and 2-4 parts of a zinc fertilizer. The complex microbial inoculant is prepared by mixing bacillus subtilis, Burkholderia cepacia, streptomyces albidoflavus and glomus versiforme according to the mass ratio of 1: (1.2 to 2.0): (1.2 to 2.0): (1.3 to 1.8). The gardenia jasminoides waste, citrus branches and other waste are fully utilized, resource recycling is achieved, and meanwhile the cost of the fertilizer is reduced; and the prepared fertilizer can be better applied to red soil in Jiangxi province, and growth of vegetables is promoted.

The invention belongs to the technical field of plant pest biological prevention and control, and particularly discloses streptomyces albidoflavus T4, which is preserved in the China Center for Type Culture Collection on May 4, 2017, and has a preservation number of CCTCC M 2017237. The invention further discloses application of the strain in prevention and control of plant pests, soil-borne root diseases of crops and nematodes, and application of the strain in crop seed vitality improvement. The streptomyces albidoflavus T4 can be used for producing plant growth promoting substance heteroauxin and plant growth promoting biomass iron carrier, so that the root growth of crops can be remarkably promoted, the crop root vitality can be reinforced, and the crop seed vitality is remarkably improved. The streptomyces albidoflavus has an excellent repairing capability for successive cropping obstacles including root-knot nematodes and root soil-borne diseases.

The invention discloses a strain of Streptomyces albus Z9 for preventing and treating sclerotinia in sunflower seedling stage. The preservation number of the strain is CGMCC 13115, which belongs to the technical field of microorganisms. Through the implementation of experiments such as the isolation, activation and identification of the pathogenic sclerotinia of sunflower sclerotinia, the primary screening and re-screening experiments of antagonistic bacteria, and the identification and analysis of antagonistic bacteria, it was shown that the strain Z9 has a strong ability to inhibit the hyphae of Sclerotinia sclerotiorum ability to grow. The infection rate of sunflower seed husks treated by the strain Z9 fermented original bacterial liquid of the present invention is reduced by 70%, the germination promotion rate of the bacterial strain Z9 fermentation filtrate is 33.3%, and the control effect of the isolated leaves of the bacterial strain Z9 fermented original bacterial liquid 69.6%, and the pot experiment control effect of bacterial strain Z9 fermented original bacterium solution is 66.7%, which can effectively inhibit the growth and reproduction of Sclerotinia sclerotiorum. The bacterial strain of the invention has simple culture conditions, is easy to preserve, is environmentally friendly, and has good development and application value.

The invention discloses a preparation method of an apple tree canker antibiological inoculant. The method comprises the steps that apple tree canker pathogen hyphae can be directly adopted as nutrition for growth and breeding, apple tree canker pathogen hyphae can be induced to produce various exocellular enzymes for degrading pathogen cell walls, streptomyces albidoflavus Actin-1 colonized at apple tree canker scab for a long time is activated to produce spores, a spore suspension is prepared, then, the spore suspension is inoculated into a liquid seed culture medium to be fermented to obtain a fermentation seed solution, finally, the fermentation seed solution is inoculated into a solid fermentation culture medium for solid fermentation to obtain spore raw powder, and then, the spore raw powder, an ultraviolet protectant, a dispersing agent, a carrier and other auxiliaries are scientifically screened and compounded to obtain the antibiological inoculant with the multiple advantages of being good in control efficiency, high in efficiency, low in recurrence rate, high in environment adaptability, high in stability, not likely to generate drug resistance and the like; the antibiological inoculant is the first choice for biological control, and is of great significance in improving the control effect of garden stuff pathogenic bacteria such as apple tree canker, preventing pathogenic bacterium relapse and achieving environmental protection.

The invention belongs to the field of microbes, and particularly provides a microbial composition for preventing and treating bacterial wilt of tobacco. The microbial composition is formed by combining three actinomyces bacteria of streptomyces microflavus CGMCC (China General Microbiological Culture Collection Center) 12841, streptomyces albidoflavus CGMCC 12842 and streptomyces pratensis CGMCC 12843 and one pseudomonas aeruginosa. The microbial composition can be used for effectively preventing and treating the bacterial wilt of the tobacco, is used for ameliorating the agronomic traits of the tobacco, adjusting the proportion of a beneficial microbe in soil and improving the yield of the tobacco and the proportion of superior cigarette. In addition, the invention also provides a culture method of the microbial composition.

The invention discloses bacillus siamese B-3 and application thereof. The Bacillus siamese B-3 disclosed in the present invention has a preservation number of CGMCC No. 13753 in the General Microorganism Center of China Microbiological Culture Collection Management Committee. Experiments have shown that the inoculum obtained by culturing Bacillus siamese B‑3 can inhibit Streptomyces scabies, Streptomyces acidiscabies and / or Streptomyces albidoflavus, which cause potato scab growth, show that Bacillus siamese B-3 of the present invention and related bacterial agents can be used to prevent and treat potato scab.

The invention belongs to the field of agricultural microorganisms, and discloses preparation and application of a streptomyces complex microbial inoculant. According to the streptomyces albidoflavus W68 provided by the invention, seeds are prepared by adopting a liquid-solid two-phase method, wherein a solid seed culture can be continuously subcultured for three times. The prepared streptomyces albidoflavus microbial agent is compounded with bacillus to prepare the compound microbial agent, so that the compound microbial agent not only has the effect of preventing and treating plant diseases, but also can improve the yield, and has better economic benefits.

The invention relates to a Streptomyces alboflavinum and a method and application of using the microorganism to prepare a biocontrol microbial agent. The preparation method comprises the following steps: placing the Streptomyces alboflavinus in a preservation medium, activating it at 28-32°C for 5-7 days, and then placing it in seeds culture medium at 30°C and 160-180r / min for 36-48 hours to obtain seed bacterial liquid; inoculate the seed bacterial liquid into the fermentation medium, ferment and cultivate in a constant temperature incubator at 30°C for 7 days, and then ferment and cultivate A good substrate is placed in a ventilated oven at 25-35°C and / or an air source heat pump dryer to dry or naturally dried at room temperature, and then pulverized to obtain a biocontrol microbial agent. The biocontrol microbial bacterial agent prepared by the method can be applied in antagonism to R. solanacearum, can be applied in salt tolerance, drought tolerance, and dissolution of soil inorganic phosphorus, and can be applied in synthesizing siderophores and growth hormone indole acetic acid, It can also be used to promote the germination and growth of tomato seeds in plants.

The present invention discloses a Trichoderma asperellum strain Sanju-19, which has been preserved in the General Microorganism Center of China Committee for Microbial Culture Collection (abbreviated as: CGMCC) on June 14, 2018, and the preservation number is It is CGMCC No.15870, and the depository address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The bacterial strain can inhibit the growth of Streptomyces scab, Streptomyces acid scab and Streptomyces albicans, and play an effective role in prevention and treatment of potato scab.