33 results about "Streptomyces albidoflavus" patented technology

The invention discloses streptomyces albidoflavus and application thereof in production of epsilon polylysine, and belongs to the field of microbial fermentation engineering. The Streptomyces albus GS114 provided by the invention can grow on an agar culture medium containing more than 10 [mu] g / ml of one or more of streptomycin, gentamycin and rifamycin, shows very strong antibiotic resistance, can normally grow in a liquid culture medium with a pH value of 2.5-3.0 and can synthesize a large amount of epsilon PL, and after fermentation for 200 h, the Streptomyces albus GS114 can be used as anantibiotic. The concentration of epsilon polylysine in the fermentation liquor is 65.7 g / L, and the yield of epsilon polylysine reaches 80.0 g / L or above after fermentation is conducted for 240 h. Thestrain also has good passage stability, and is an epsilon PL industrial production strain with great potential.

The invention belongs to the technical field of primer design, and discloses a primer set, the primer set is P2939123F and P2939374R and / or P1751437F and P1751612R. The primer set provided by the invention has strong specificity, high sensitivity and high detection efficiency, and can be used to effectively identify Streptomyces albus flavinus W68.

The invention discloses a preparation method of an apple tree canker antibiological inoculant. The method comprises the steps that apple tree canker pathogen hyphae can be directly adopted as nutrition for growth and breeding, apple tree canker pathogen hyphae can be induced to produce various exocellular enzymes for degrading pathogen cell walls, streptomyces albidoflavus Actin-1 colonized at apple tree canker scab for a long time is activated to produce spores, a spore suspension is prepared, then, the spore suspension is inoculated into a liquid seed culture medium to be fermented to obtain a fermentation seed solution, finally, the fermentation seed solution is inoculated into a solid fermentation culture medium for solid fermentation to obtain spore raw powder, and then, the spore raw powder, an ultraviolet protectant, a dispersing agent, a carrier and other auxiliaries are scientifically screened and compounded to obtain the antibiological inoculant with the multiple advantages of being good in control efficiency, high in efficiency, low in recurrence rate, high in environment adaptability, high in stability, not likely to generate drug resistance and the like; the antibiological inoculant is the first choice for biological control, and is of great significance in improving the control effect of garden stuff pathogenic bacteria such as apple tree canker, preventing pathogenic bacterium relapse and achieving environmental protection.