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Nematicidal streptomyces albidoflavus and application thereof

A technology for Streptomyces albus and Knot nematodes, applied in the direction of nematicides, applications, bacteria, etc., can solve problems such as unsuitable for sustainable development of agricultural and forestry production, chemical control restrictions, nematode resistance and other problems

Inactive Publication Date: 2017-07-14
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical nematicides are highly toxic, cause serious environmental pollution, are unsafe to humans and animals during use, have poor ecological benefits, and are likely to cause nematodes to develop resistance. Many nematicides have been banned
The most important thing is that there is a potential threat to the health of humans and animals, and it also leads to increasingly serious side effects such as high pesticide residues in fruits and killing natural enemies. With the enhancement of public environmental protection awareness and the continuous improvement of their own quality of life requirements, chemical control is increasingly restricted. To meet the needs of sustainable development of agricultural and forestry production
Although methods such as crop rotation, planting disease-resistant varieties and improving cultivation measures have different degrees of control effects on nematodes, they all have relatively large limitations and cannot meet the requirements of modern agricultural production. Therefore, in addition to continuing to explore some high-efficiency, low-toxicity, and In addition to low-residue, high-selectivity nematicides, the focus has shifted to seeking new methods from an ecological point of view. Screening beneficial microorganisms for biological control of root-knot nematode diseases is one of them. Biological control is stable, economical, and long-lasting. And relatively safe features, while reducing pollution and protecting the ecological environment

Method used

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  • Nematicidal streptomyces albidoflavus and application thereof
  • Nematicidal streptomyces albidoflavus and application thereof
  • Nematicidal streptomyces albidoflavus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and Identification of Streptomyces albidoflavus BN-F2 CGMCC No.12741

[0031] 1. Isolation and purification of BN-F2

[0032] 1. Collection and isolation method of Streptomyces microalbubus BN-F2

[0033] 10g of soil was collected from the Dongdadi teaching base of Beijing Agricultural College, added to a triangular flask containing 100mL of sterile water, and shaken on a shaker at 120rpm for 30min to make a soil suspension. mL of sterile water, vortexed, serially diluted 10-1 -10 -8 times, 150 μL of the diluted solution was spread on the Gaoshi No. 1 medium plate, cultured at 28°C for 3 days, and a single colony on the medium was picked and purified by streaking on the plate to obtain the strain of Streptomyces albus.

[0034] 2. Identification of strain BN-F2

[0035] 1. Morphological observation of strains

[0036] (1) Observation by optical microscope: Observing the microscopic morphological characteristics of the strain by inserting the pla...

Embodiment 2

[0130] Example 2. Determination of the insecticidal activity of Streptomyces albidoflavus (Streptomyces albidoflavus) BN-F2CGMCC No.12741 The bacterial strain obtained in Example 1 was tested for activity, and the specific method is as follows:

[0131] 1. Preparation of fermentation broth of strains to be tested

[0132] Insert the actinomycetes to be tested into Gao’s No. 1 medium, shake and culture at 25°C and 180rpm for 6 days, centrifuge with a Beckman centrifuge at 10,000rpm for 20min, remove medium residues and hyphae, and absorb the supernatant to obtain fermentation solution, kept refrigerated for use in the determination of insecticidal activity.

[0133] 2. Preparation of root-knot nematodes for testing

[0134] Take the diseased root of tomato root-knot nematode, rinse it gently with tap water, gently pick out the milky white oocyst on the diseased root with a dissecting needle, put it into a small petri dish with a diameter of 6 cm, add a small amount of sterile ...

Embodiment 3

[0143] Embodiment 3. Streptomyces albidoflavus (Streptomyces albidoflavus) BN-F2CGMCC No.12741 fermentation condition optimization

[0144] Inoculate Streptomyces microalbubus BN-F2 on different fermentation media, at different fermentation temperatures, different fermentation times, different rotation speeds of shaking tables, different inoculum sizes, different initial pH values ​​of culture media, different carbon sources and different nitrogen sources. Fermentation culture on the medium, and then according to the method described in Example 2, measure the insecticidal activity of the obtained fermentation liquid to root-knot nematodes, and deduce the optimal medium and fermentation conditions.

[0145] 1. Screening of fermentation medium

[0146] 1. Effect of different carbon sources on nematicidal activity

[0147] Quantitatively inoculate the strains to be tested on different culture media with soluble starch, glucose, sucrose, mannitol, corn flour, oatmeal and glycerol...

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Abstract

The invention discloses a nematicidal streptomyces albidoflavus (Streptomyces albidoflavus) and an application thereof in control of plant root-knot nematodes. The preservation number of the streptomyces albidoflavus is CGMCC No.12741. The streptomyces albidoflavus is separated from a soil sample. A fermentation liquid of a bacterial strain has obvious insecticidal activity on the plant root-knot nematodes and can be used for preparing a microbial agent for controlling the plant root-knot nematodes.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of nematicidal Streptomyces albicans and its application. Background technique [0002] Plant root-knot nematodes (Meloidogyne spp.) are a class of plant parasitic nematodes that cause serious losses in agricultural production worldwide. It belongs to Secernentea, Tylenchida, Heteroderoidea, and Nematodes (Meloidogyne), root-knot nematodes (Meloidoyne Goeldi, 1989). At present, there are more than 90 effective species reported in the world, among which 4 species are the most widely distributed and most harmful to crops, they are M.incognita incognita, M.javanica and M. M.arenaria and the northern root-knot nematode M.hapla, more than 90% of plant root-knot nematode diseases are caused by these four nematodes, especially the southern root-knot nematode. [0003] Root-knot nematodes mainly damage the roots of plants. They use needles to suck the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/465
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 魏艳敏任争光孔兵兵贺佳谢心悦贾慧慧闫哲石芮竹
Owner BEIJING UNIV OF AGRI
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