The invention discloses a bactericidal composition containing cupric nonyl phenolsulfonate and a methoxy-acrylate bactericide. The methoxy-acrylate bactericide is selected from azoxystrobin, kresoxim-methyl, metominostrobin, fluoxastrobin, trifloxystrobin, orysastrobin, picoxystrobin, pyraclostrobin, enostrobilurin, SYP-1620, phenylatestrobin, coumoxystrobin, pyraoxystrobin and pyraclostrobin, wherein the weight ratio of the cupric nonyl phenolsulfonate to the methoxy-acrylate bactericide is 1:5-20:1. The bactericidal composition can be prepared into missible oil, microemulsion, water-dispersible granules, wettable powder and a suspending agent which are permitted in the agriculture. The bactericidal composition containing the cupric nonyl phenolsulfonate and the methoxy-acrylate bactericide is reasonable in ingredients, good in bactericidal effect and low in drug cost, and the activity and the bactericidal effect are not formed by the activities of the ingredient simply. Compared with an existing single preparation, the bactericidal composition has remarkable synergistic effect except the remarkable bactericidal effect, is free of pollution to crops and good in safety, meets the safety requirements of pesticide preparations, and has remarkable preventive and control effect on powdery mildew, downy mildew, anthracnose and banded sclerotial blight of the crops.