48 results about "Chilo suppressalis" patented technology

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

The invention discloses a chilo suppressalis indoor artificial feeding feed and a feeding and reproduction method, and relates to the technical field of insect feeding. By optimizing the proportion of the indoor artificial breeding feed for the chilo suppressalis and the breeding method, the method has the following technical effects that (1) the survival rate of larvae within 15 days reaches 100%, and the survival rate of the larvae is increased; (2) the optimized feed formula can meet the nutritional requirements of larvae in each stage, the taste is good, the feed attraction is strong, and the synchronous development is promoted; (3) the breeding efficiency is improved; (4) the disease and pest risk is reduced, and the disease occurrence rate is lower than 5%; and (5) ensuring the quality of the insect source: the obtained chilo suppressalis insect source is consistent in physiological state and neat in development, the insect body conversion rate reaches 69.6%, and the insect use requirements of large-scale experiments are met.

The present invention relates to a prediction method for the occurrence of Chilo suppressalis in rice, including: constructing a data set based on the date of rice growth and development period, the number information of Chilo suppressalis, and meteorological environment data, and dividing it into a training set and a test set; based on the random forest model, using grid search and cross-validation to determine the input parameters and parameter thresholds of the model, and determining the best numerical combination of the model input parameters; respectively predicting the cumulative number of Chilo suppressalis in the training set and the test set; using evaluation indicators to evaluate the training set and the test set respectively to obtain the goodness of fit of the model and the correlation degree between the predicted value and the actual value; using the test set to analyze the trend of the cumulative number of Chilo suppressalis. The beneficial effects of the present invention are: the prediction method for the occurrence of Chilo suppressalis in rice provided by the present invention can overcome problems such as traditional pest prediction relying on experience, low efficiency, and high uncertainty, and realize the prediction of the cumulative number of Chilo suppressalis in the time series of rice.

The invention discloses an application of a volatile substance in prevention and control of agricultural insect chilo suppressalis, and provides an application of (E)-2-hexenal (trans-2-hexenal) in prevention and control of chilo suppressalis for the first time, so that chilo suppressalis adults can be effectively repelled, the egg laying amount of chilo suppressalis female insects can be inhibited, and the prevention and control of chilo suppressalis can be realized. The natural enemy insects attracting the chilo suppressalis comprise egg parasitic wasp trichogramma japonicum and larva parasitic wasp cotesia chilo suppressalis. The (E)-2-hexenal can reduce the egg quantity of chilo suppressalis in the field, reduce the occurrence cardinal number of the chilo suppressalis, play a prevention and control role and reduce loss before pests cause harm, and meanwhile, the (E)-2-hexenal can attract natural enemy insects of the chilo suppressalis, so that natural enemies in the natural environment can be fully utilized and protected, and the economic benefit of the chilo suppressalis is improved. The improvement of the chilo suppressalis prevention and control effect and the green prevention and control are realized. (E)-2-hexenal is a component in rice volatile matters, and compared with chemical pesticides, (E)-2-hexenal is more beneficial to the ecological environment.

The present invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, an N-oxide or salt thereof and the use thereof. The compound is as shown in general formula (I), wherein X represents cyano, cyanoalkyl, aryl, or heterocycly; Q1 and Q2 each independently represent O or S; M1, M2, M3, M4 and M5 each independently represent CR9 or N, and at least two of them are N; R1, R2, R3, R4, R5, R6, R7, R8 and R9 each independently represent hydrogen, nitro, amino, cyano, halogen, etc. The compound has an excellent control effect on pests such as Chilo suppressalis, Spodoptera frugiperda, Mythimna separata, Spodoptera litura, Aphis craccivora, rice planthopper, etc., and is safe for bees.

The invention belongs to the technical field of pesticides, and particularly relates to a heterocyclic substituted pyridine compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein R1, R2, R4, R5, R6, R8, R9 and R10 respectively and independently represent hydrogen, halogen, alkyl, alkenyl and the like; r3 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group or the like; and R7 represents halogen. The compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, beet armyworm, agrotis ypsilon, chilo suppressalis, plutella xylostella, brown planthopper, rhopalosiphum padi and the like.

The invention relates to the field of agricultural pest drug resistance detection, and provides a detection primer and method for chilo suppressalis ryanodine receptor I4758M mutation and application. According to the detection primer, a mutation recognition site is designed at the 3'tail end of an inner primer BIP, specific detection is achieved through the key base difference (A / G) of a wild type and a mutant type, and the primer group comprises an outer primer pair F3 / B3, an inner primer pair FIP / BIP and a loop primer pair LoopF / LoopB; the detection method is based on loop-mediated isothermal amplification (LAMP), the genotype is visually interpreted through color change of reaction liquid, a thermal cycler is not needed, and the detection method has the advantages of being high in accuracy and sensitivity, easy to operate, low in cost and the like. The matched kit comprises a rapid DNA extraction assembly and is suitable for field on-site detection. The gene mutation frequency of the specific site of the chilo suppressalis can be rapidly detected, and the technical support is provided for resistance monitoring, early warning and scientific treatment of bisamide insecticides.

The invention relates to a chilo suppressalis seedless sperm formation factor TNNI3 and application thereof in male sterility. The invention finds that the TNNI3 gene specifically regulates and controls the chilo suppressalis seedless spermatogenesis process for the first time, and an RNA interference technology proves that TNNI3 can inhibit the number of chilo suppressalis seedless sperm bundles and has no obvious influence on the number of nucleated sperm bundles and sperm motility, so that the egg laying amount after mating of male insects and wild female insects can be obviously reduced, and the survival rate of the male insects and the wild female insects is improved. And a theoretical basis is provided for developing a novel prevention and treatment strategy for male reproduction.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

The invention belongs to the technical field of synthesis, and particularly relates to a small reactor for preparing a chilo suppressalis pheromone intermediate and application of the small reactor. According to the small reactor, rapid mixing is achieved through a threaded mixing pipe, the introduction amount of a hydrobromic acid solution is controlled in the mixing process, and a reaction from low concentration to unilateral bromination of hydrobromic acid is achieved; meanwhile, a mixed solution obtained after the bromination reaction is mixed with a sodium bicarbonate solution in a spraying mode, the washing neutralization effect is achieved, standing, cooling and layering are formed through pressure release valves arranged in a gradient mode, and finally a methylbenzene solution and an aqueous solution are obtained. The method is mild in reaction condition, short in reaction time, high in reaction efficiency, high in automation degree, low in energy consumption and good in safety, meets the green chemical engineering concept and is easy to industrially apply.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention provides a pesticide composition containing a pyrethroid compound, which consists of a compound with a structural formula (I) and a second effective component, the second effective component is bifenthrin, beta-cyhalothrin, fenpropathrin, beta-cyhalothrin, beta-cyhalothrin, beta-cyhalothrin, cypermethrin, cyfluthrin, deltamethrin, ethofenprox, fenvalerate or cis-cypermethrin, and the weight ratio of the bifenthrin, the beta-cyhalothrin, the fenpropathrin, the fenvalerate and the cis-cypermethrin is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially phyllotreta striolata, ostrinia nubilalis or tetranychus cinnabarinus. The invention verifies that a remarkable synergistic effect can be obtained by compounding the flufenoxanil and the pyrethroid insecticide in a proper proportion, the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are realized, and the composition has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle and tetranychid mites, and has a good control effect on the target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis. (I).

The invention provides an aryl isoxazoline compound as well as an isomer or pharmaceutically acceptable salt thereof and application thereof, the structure of the aryl isoxazoline compound is shown as a formula (I), in the formula (I), R1, R2, R3, R4 and R5 are respectively and independently selected from hydrogen atoms, halogen atoms, cyano groups and other groups, and R1, R2, R3, R4 and R5 are independently selected from hydrogen atoms, halogen atoms, cyano groups and other groups; l1 is an alkylene group of a C1-C6 straight chain or branched chain; and R6 is selected from a substituted or unsubstituted C2-C8 alkenyl group, a substituted or unsubstituted C2-C8 alkynyl group, a substituted or unsubstituted C3-C6 cycloalkyl group, a substituted or unsubstituted C1-C4 alkylene group, a C3-C6 cycloalkyl group or C (= O) R7. The aryl isoxazoline compound has no cross resistance with existing insecticides, has an efficient control effect on pests, and particularly has an excellent pesticide effect on rice-stem borers. In addition, the compound is low in toxicity, environment-friendly and suitable for large-scale application.

The invention discloses a rapid visual detection kit for drug resistance of chilo suppressalis bisamide insecticides and application of the rapid visual detection kit. According to the invention, by utilizing the synergistic effect of RPA and CRISPR / Cas12a systems, Y4667D and I4758M mutation sites closely related to resistance in chilo suppressalis ryanodine receptor genes are specifically targeted, an RPA primer pair and crRNA are provided, a one-pot detection method is established, a reaction can be triggered only by manual uniform mixing at a constant temperature, and a centrifugation step is not needed; a coarsely extracted DNA sample can be directly used, so that a tedious purification process is omitted; in combination with a fluorescently-labeled DNA probe, chilo suppressalis individuals carrying key resistance mutations and sensitive individuals can be quickly identified and distinguished with high sensitivity, and the result can be directly observed and interpreted by naked eyes. A visual detection scheme which is extremely simple and convenient to operate, rapid in reaction, excellent in specificity and economical in cost is provided for monitoring chilo suppressalis bisamide insecticide resistant populations in the field in real time.

The utility model belongs to the field of chilo suppressalis, and particularly relates to a rice chilo suppressalis pupation and eclosion behavior observation device which comprises a base and a feeding device, the feeding device is installed on an installation plate, the feeding device is composed of seven feeding boxes, and pupation plates are arranged in the feeding boxes; the utility model provides a rice chilo suppressalis pupation and eclosion behavior observation device which comprises a feeding device and a putting device, the feeding device comprises seven feeding boxes, and the putting device is arranged in the feeding device and used for dividing chilo suppressalis larvae into seven parts according to the head width. The chilo suppressalis larvae can be divided into seven parts according to the width of head shells, each part of chilo suppressalis larvae is independently fed, and the pupation and eclosion behaviors of chilo suppressalis can be better observed through the transparent feeding device and the pupation plate; and the rotatable mounting plate can reduce the labor force of observers, so that the observers can better observe the chilo suppressalis.

The invention belongs to the technical field of crop disease and insect pest control, and relates to a method for efficiently controlling first-generation chilo suppressalis by feeding transplanting pesticide. The method mainly comprises the following steps: firstly, scientifically selecting an insecticide which is good in uptake conductivity, long in lasting period, low in drug resistance level and high in safety, then, carrying out insecticide treatment on a rice seedling bed in a conventional insecticide application mode such as spraying or broadcasting before transplanting the rice seedlings, and then, carrying out mechanical transplanting on the rice seedlings subjected to the insecticide treatment in a field. Compared with the existing chilo suppressalis prevention and control technology, the method provided by the invention has the advantages that the investigation process for the appropriate pesticide application period is omitted, the operation area is obviously reduced, the influence of complex conditions such as field terrain, geology and environment on the pesticide application operation is avoided, the seedlings can be ensured to be robust without insect pests, and the pesticide consumption is reduced. The method for efficiently preventing and treating the first-generation chilo suppressalis by feeding the pesticide before grafting is simple and convenient to operate, time-saving, labor-saving, economical and efficient, and has a wide application prospect in the aspects of green prevention and control of rice diseases and insect pests, agricultural emission reduction, sustainable agriculture and the like.

The present invention discloses an insecticide synergistic composition containing a piperic acid derivative compound and an application thereof. The composition consists of two active ingredients, compound A and compound B, wherein the active component A is compound I-72; the active component B is selected from any one of indoxacarb, s-indoxacarb or metaflumizone; the compound I-72 has the following structure: The composition of the present invention has the advantages of obvious synergy, delayed resistance, etc., and can be used for preventing and controlling lepidopteran and coleopteran pests on agriculture, forestry and ornamental plants, especially for use on resistant striped stem borer and resistant diamondback moth.

The invention discloses a KASP labeled primer for detecting resistance of chilo suppressalis to chlorantraniliprole insecticide and application of the KASP labeled primer, and relates to the technical field of agricultural biology. The KASP labeled primer group disclosed by the invention comprises a primer combination which is used for detecting the resistance mutations of the chlorantraniliprole insecticide, such as CsupI4758M, CsupY4667C, CsupY4667D, CsupY4894F and CsupG4915E. The group of KASP labeled primers is good in specificity and high in sensitivity, and can accurately detect the resistance mutation frequency of the chilo suppressalis to the chlorantraniliprole insecticide.

The application discloses an isoxazoline compound as shown in formula (I) and application thereof, wherein R1, R2 and R3 can be selected from halogen atoms, a cyano group, a nitro group, C1-C3 halogenated alkyl and the like; Q can be independently selected from A1-A6; L1 and L2 can be selected from a carboxyl group, C1-C6 alkoxycarbonyl, a C1-C8 alkyl group substituted by a hydroxyl group and the like; Z1 and Z2 can be selected from a hydrogen atom, a C1-C6 alkyl group, a C3-C5 cycloalkyl group or C(O)M, wherein M is a C1-C6 alkyl group, a C2-C6 alkenyl group and the like; the isoxazoline compound provided by the application has no cross resistance with existing insecticides, has the advantages of fast drug efficacy, low dosage and the like, and has excellent prevention and treatment effects on rice resistant Chilo suppressalis, Plutella xylostella, aphids and the like. The application further provides the isoxazoline compound as a salt acceptable to a pesticide, a composition and application.

The invention relates to a pesticide composition containing a nicotine compound and application of the pesticide composition. The pesticide composition is prepared from flufenican amide, acetamiprid, thiamethoxam or clothianidin and the like. The pesticide composition has an obvious synergistic effect, the usage amount of pesticides is reduced, the residual amount of the pesticides on crops is reduced, and the environmental friendliness is enhanced. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae.

The invention provides a pesticide composition containing antibiotic compounds, the pesticide composition is composed of a first effective component and a second effective component, the first effective component is a compound with a structural formula (I), the second effective component is methylamino abamectin benzoate, abamectin, spinosad, spinetoram or ivermectin, and the weight ratio of the first effective component to the second effective component is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention of agricultural and forestry pests. The invention verifies that the binary composition obtained by compounding the flufenutraniliprole and the antibiotic insecticide in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips and flea beetle. (I).

The invention discloses a method for effectively reducing the overwintering cardinal number of chilo suppressalis in rice-wheat continuous cropping areas of Huanghuai and northern China, which comprises the following steps: (1) harvesting without stumps, controlling the height of rice stumps within 5cm, and completely crushing straws left in the field; (2) after harvesting, performing full-coverage spraying prevention and control on the'empty stubble 'field of the rice, and comprehensively sealing and killing an overwintering place to kill field insect sources; (3) performing rotary tillage and deep tillage on the empty stubble field after spraying before winter, wherein the rotary tillage depth is about 30cm; the method is suitable for large-area popularization and application in rice and wheat continuous cropping areas in Huanghuai and northern China, the death rate of overwintering generation mature larvae is controlled to be 96% or above, traditional high stubble is changed into zero stubble harvesting, pupa killing in deep water after spring is changed into pest killing before winter, treatment is conducted in the non-plant growth period, operation is easy, time is saved, water irrigation is not needed, and water sources are saved; early stubble removal, early planting and early income are achieved.

The application discloses a Beauveria bassiana strain SL-066 and application thereof, and relates to the technical field of biological strains. The strain provided by the application is preserved in the China General Microbiological Culture Collection Center on August 8, 2022, and the preservation number is CGMCC No. 40269. The Beauveria bassiana strain SL-066 provided by the application can effectively prevent and control various rice pests, is a Beauveria bassiana strain capable of preventing and controlling the coleopteran pest Curculionidae (rice water weevil adult), the coleopteran pest Chrysomelidae (rice water weevil larva) and the lepidopteran pest Chilo suppressalis (Chilo suppressalis larva), and has a good prevention and control effect on the three common pests on rice.

The invention discloses a chilo suppressalis gene CsFer1, a coding product of the gene CsFer1 and application of the gene CsFer1 to prevention and treatment of chilo suppressalis. The gene CsFer1 has a DNA sequence as shown in SEQ ID No.1. The gene sequence is a complete coding frame composed of 699 nucleotides, and small molecular weight protein containing 232 amino acid residues is coded. Research finds that the gene is related to growth and development of chilo suppressalis. An RNAi carrier of CsFer1 is constructed and transferred into rice, dsRNA of the CsFer1 gene is expressed in transgenic rice, and the purpose of improving the resistance of rice to chilo suppressalis can be achieved. The invention will be widely applied in crop breeding, especially in rice insect-resistant breeding.

The invention provides a method for reducing the lethal risk of an insecticide to cotesia chilo borers, and belongs to the technical field of comprehensive treatment of agricultural pests, the method comprises the following steps: treating fourth-instar larvae of chilo suppressalis by adopting cyprofluramid; and parasitizing the fourth-instar larvae of the chilo suppressalis treated by the cyprofluramid by adopting the female cotesia chilo suppressalis in a ratio of 1: 1, and taking out the female cotesia chilo suppressalis after parasitism is completed. The method not only can reduce the adverse effect of the cyproflufen on the cotesia chilo borer, but also can effectively protect the natural enemy from being damaged by the pesticide, thereby maintaining the population dynamics of the natural enemy, being beneficial to maintaining the ecological balance of the farmland and reducing the pressure of the pesticide on the environment, being in line with the strategic direction of green agriculture and agricultural sustainable development, and having wide application prospects. The implementation method is simple and facilitates popularization and application.

The invention relates to pyridino [1, 2-a] pyrimidine mesoion derivatives containing a pyrazole structure as well as a preparation method and application of the pyridino [1, 2-a] pyrimidine mesoion derivatives. The compound disclosed by the invention has a structure shown as a formula (I), has excellent insecticidal activity on spodoptera frugiperda, chilo suppressalis, plutella xylostella, ostrinia nubilalis, aphis aphis and the like, and can be used for preparing drugs or medicaments for preventing and treating pests such as spodoptera frugiperda, chilo suppressalis, plutella xylostella, ostrinia nubilalis, aphis aphis and the like. The structure and the preparation process are simple, and the production cost is low. (I).

The invention belongs to the technical field of microbial pesticides, and particularly relates to a special synergistic aid lecithin for metarhizium anisopliae Mr006. The lecithin additive has good compatibility with the metarhizium anisopliae Mr006, spore production can be effectively promoted, the insecticidal activity of the metarhizium anisopliae is remarkably improved, the infection time of target pests is effectively shortened, and the insecticidal time efficiency of the metarhizium anisopliae is improved. The metarhizium anisopliae Mr006 and lecithin are combined for use, and lecithin has a synergistic effect on the metarhizium anisopliae Mr006, so that the control effect on beet armyworms and chilo suppressalis can be improved. Meanwhile, the lecithin additive is relatively high in safety to non-target organisms, so that a new technical support is provided for the development of green agriculture.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a metarhizium anisopliae engineering bacterium for expressing spider venom peptide Hv1a and application of the metarhizium anisopliae engineering bacterium in prevention and control of chilo suppressalis. The invention discloses the obvious insecticidal activity of Metarhizium guerizense ARSEF 977 on chilo suppressalis for the first time, on the basis, an Hv1a toxic peptide gene derived from a spider is introduced into the strain through a genetic engineering technology, an engineering bacterium for stably and efficiently expressing the spider insecticidal peptide Hv1a is successfully constructed, the engineering bacterium plays a synergistic effect, the insecticidal effect on the chilo suppressalis is further improved, and the application prospect is wide. The death rate after 7 days of treatment reaches up to 96.7%, and the method can be used for controlling chilo suppressalis. The invention provides a high-potential starting strain for developing a special fungal insecticide for chilo suppressalis, provides a new material and method for promoting the development of a green and efficient chilo suppressalis prevention and control technology, and has a wide application prospect.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to application of a collagen gene Col15 in regulation and control of chilo suppressalis female sterility and green prevention and control. The key effect of Col15 in development of ovaries of chilo suppressalis is disclosed for the first time through bioinformatics identification, expression profile analysis, RNA interference and CRISPR / Cas9 gene editing technologies. Through targeted inhibition or interference of gene expression, ovarian development can be severely blocked, collagenous fiber deposition is reduced, oocyte maturation is blocked, the mating rate, the egg laying amount and the egg hatching rate of female insects are remarkably reduced, and finally female sterility is achieved. The invention provides a brand new target and technical support for developing a novel green pest prevention and control technology based on female reproduction regulation, has the characteristics of accurate technical effect, environment friendliness and strong sustainability, and has wide application prospects in biological treatment of pests.