The invention discloses reference genes of Metopolophium dirhodum, and sequences, a screening method and application thereof, belongs to the field of molecular biology, and particularly relates to thereference genes Actin, GAPDH, NADH, AK, SDHB, RPL8, EF1A and RPL4 of the Metopolophium dirhodum. Nucleotide sequences of the reference genes are respectively shown as SEQ ID No.1, SEQ ID NO.4, SEQ IDNO.7, SEQ ID NO.10, SEQ ID NO.13, SEQ ID NO.16, SEQ ID NO.22 and SEQ ID NO.25. The nucleotide sequences of the reference genes of the Metopolophium dirhodum can provide a useful method for research of gene expression of functional genes of the Metopolophium dirhodum or different geographical populations, development stages and body parts in the future, or for different-temperature treatment, or for starvation treatment, or for insecticide treatment, or for antibiotic treatment, or for research of gene expression of winged and winingless Metopolophium dirhodum.