Treatment of transgenic corn seeds with pesticide
A technology of genetically modified corn and pesticides, applied in biocides, genetic engineering, plant genetic improvement, etc., can solve the problems of not providing, not pointing out, limiting successful use, etc., and achieve the effect of reducing the application ratio
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Embodiment 1
[0097] Corn seeds were prepared with transgenic events effective against corn insectivores and such seeds were treated with imidacloprid (Gaucho(R)) and tefluthrin (Raze(R)).
[0098] Maize seeds were prepared to express the Bacillus thuringiensis endotoxin Cry3Bb.11231 or Cry3Bb.11098 by the method described in WO 99 / 31248 for these respective events.
[0099] Maize seeds of the same hybrid species with or without each transgenic event were treated with imidacloprid (available as GAUCHO® from Bayer Corp.) or tefluthrin (available as RAZE® from Wilbur-Ellis Co., Great Falls) as described below. , Mont.; Walla Walla, WA). Seed treatment formulations of the desired insecticides were prepared by mixing measured amounts of the insecticide in water as a carrier and applying the resulting formulations to measured weights of maize seeds in a rotary static seed treater at room temperature for 1 minute. The respective weights of the insecticide formulation and corn seed are calculate...
Embodiment 2
[0103] A field trial to determine the efficacy of transgenic event Cry3Bb.11231 in combination with corn insecticide seed treatments in corn seeds against western and northern corn insectivores.
[0104] Conduct field trials in accordance with the protocol and in compliance with USDA notification requirements. The purpose of these experiments was to determine the efficacy of the combination of the transgenic event Cry3Bb.11231 in corn seeds with a corn insecticide seed treatment against western and northern corn insectivores.
[0105] For each growing location selected, the plot design included the following: Row Spacing: 30 inches Trial Size: 4 Rows x 20 Seeding Density: 2.0 Seed Feet Hybrids Used: LH198 x LH185 or RX670 Replication Trials: 4 Designs : Random complete block Location: 4 Larval source: Supplement natural infection by artificial infection with 400 eggs / ft (growth stage V2)
[0106] Use the following seed treatment combinations for each growing area: Numbering...
Embodiment 3
[0121] A field trial to determine the efficacy of transgenic event Cry3Bb.11231 in combination with imidacloprid seed treatment in maize seed against western and northern maize carnivores.
[0122] Conduct field trials in accordance with the protocol and in compliance with USDA notification requirements. The purpose of this trial was to determine the efficacy of the combination of the transgenic event Cry3Bb.11231 in maize seeds with maize insectivorous seed treatment with imidacloprid.
[0123] For each growing location selected, the plot design included the following: Row Spacing: 30 inches Trial Size: 4 Rows x 20 Seeding Density: 2.0 seeds / foot Hybrids Used: LH198 x LH185 or RX670 Replication Trials: 4 Design: Randomized complete block Location: 4 Larval source: Supplement natural infection by artificial infection at 400 eggs / ft (growth stage V2)
[0124] Use the following seed treatment combinations for each growing area: Numbering corn seed type Insecticide and amo...
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