8 results about "Artificial infection" patented technology

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

The present invention discloses the application of a strain in constructing an animal pneumonia model and a construction method, and belongs to the technical field of animal model construction. By adopting the infection strain and construction method provided by the present invention, it is not necessary to inject rats with immunosuppressants such as cyclophosphamide before infection. The rats only need to be adaptively raised to be successfully infected and modeled, which simulates the real infection scene to the maximum extent, shortens the experimental cycle, and saves the experimental cost; the constructed rat pneumonia model is stable for a long time, and by the end of the observation, the lung pathological symptoms and blood infection symptoms have always existed, the sick rats do not heal themselves, and the lungs are severely damaged; the infection strain and construction method provided can provide a reference for the establishment of other bacterial pneumonia models, thereby further laying the foundation for the development of pneumonia drugs and the research on their mechanism of action, and providing more basis and inspiration for clinical guidance of medication.

This invention relates to the field of molecular marker breeding technology, and more particularly to a functional molecular marker for rapid identification of largemouth bass resistant to rhabdovirus and its application. This invention develops a functional molecular marker that identifies significantly different molecular markers by resequencing the genomes of resistant and susceptible individuals. Specific primers were designed, and combined with PCR amplification and agarose gel electrophoresis, successfully distinguishing between resistant and susceptible individuals. In the experiment, largemouth bass fry were challenged with rhabdovirus through artificial infection to identify the susceptible group (SL) and the resistant group (RL). RNA extraction and qPCR were used to detect differences in viral load, and PCR amplification and gene sequencing analysis were performed on resistant and susceptible individuals to verify the accuracy of the functional marker. The amplification products of resistant and susceptible individuals were single bands of 1273 bp and 1134 bp, respectively, showing significant genetic differences and providing technical support for rapid screening of resistant individuals.

The invention relates to the technical field of aquaculture, in particular to application of heparan sulfate in prevention and treatment of grass carp reovirus. The heparan sulfate is competitively combined with the grass carp reovirus, so that the virus adsorption and infection process can be effectively inhibited. In drugs, fish feed and additives, when the concentration of heparan sulfate exceeds 0.1 mu g / mL or 10mg / kg, the effect of resisting grass carp reovirus is obviously shown. Cell experiments and artificial infection challenge experiments prove that the virus infection rate can be reduced, the survival rate can be increased, and tissue damage can be reduced. As an antiviral feed additive, the heparan sulfate provided by the invention has the advantages of low cost, small dosage, high safety and the like, provides a new strategy for preventing and treating viral diseases in the aquaculture industry, and has a wide application prospect.

The invention relates to the technical field of molecular marker breeding, in particular to a functional molecular marker for rapidly identifying largemouth bass rhabdovirus-resistant individuals and application of the functional molecular marker. The invention develops a functional molecular marker, the molecular marker with significant difference is identified by re-sequencing genomes of disease-resistant and susceptible individuals, specific primers are designed, and the disease-resistant and susceptible individuals are successfully distinguished by combining PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis technologies. In the experiment process, a rhabdovirus challenge experiment is carried out on largemouth micropterus salmoides offspring seeds in an artificial infection mode, and a susceptible group (SL) and a disease-resistant group (RL) are defined. RNA (Ribonucleic Acid) extraction and qPCR (Quantitative Polymerase Chain Reaction) are adopted for detecting the difference of virus loads, and PCR amplification and gene sequencing analysis are carried out on disease-resistant and susceptible individuals, so that the accuracy of functional markers is verified. Disease-resistant and susceptible amplification products are 1273bp and 1134bp single bands respectively, show significant gene difference, and provide technical support for rapid screening of disease-resistant individuals.

This invention belongs to the fields of plant pathology and agricultural microbiology, specifically relating to a method for promoting efficient sporulation of *Anthracnose citrinum*, the obtained *Anthracnose citrinum* spore suspension, and its applications. The method involves inoculating *Anthracnose citrinum* mycelial cakes onto a high-concentration carrot agar medium, culturing them in a spore induction chamber under controlled temperature, humidity, and ventilation conditions until the mycelium covers the plate, then marking the medium with multiple parallel horizontal and vertical lines, adding glycerol aqueous solution to adjust the surface water activity to the range of 0.93–0.97, and applying a controlled spectral induction using alternating blue light and UV-A. Finally, a high-concentration spore suspension is obtained through elution. This invention significantly improves the sporulation yield and spore germination rate per plate while significantly reducing batch-to-batch variation. It is suitable for constructing standardized artificial infection models of *Anthracnose citrinum*, and can be used for pesticide efficacy evaluation, disease-resistant variety screening, and fruit storage and preservation research.

The invention belongs to the technical field of phytopathology and agricultural microorganisms, and particularly relates to a method for promoting efficient spore production of colletotrichum gloeosporioides, an obtained colletotrichum gloeosporioides spore suspension and application of the colletotrichum gloeosporioides spore suspension. The method comprises the following steps: inoculating a high-concentration carrot agar culture medium with a colletotrichum gloeosporioides cake, culturing in a spore induction cavity with controllable temperature, humidity and ventilation conditions until hyphae cover a flat plate, carrying out grid scribing according to a plurality of horizontal and vertical parallel lines, dropwise adding a glycerol aqueous solution to adjust the surface water activity of the culture medium to be 0.93-0.97, and culturing at the temperature of 20-30 DEG C to obtain the colletotrichum gloeosporioides. Controllable spectrum induction with blue light and UV-A alternating combination is applied, and finally high-concentration spore suspension is obtained through elution. According to the method, the single-plate sporulation quantity and the spore germination rate are remarkably improved, meanwhile, the batch-to-batch variation is remarkably reduced, and the method is suitable for constructing a standardized artificial infection model of the fruit anthracnose and is used for pesticide effect evaluation, disease-resistant variety screening and related research of fruit storage and preservation.