4 results about "Artificial infection" patented technology

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

This invention belongs to the fields of plant pathology and agricultural microbiology, specifically relating to a method for promoting efficient sporulation of *Anthracnose citrinum*, the obtained *Anthracnose citrinum* spore suspension, and its applications. The method involves inoculating *Anthracnose citrinum* mycelial cakes onto a high-concentration carrot agar medium, culturing them in a spore induction chamber under controlled temperature, humidity, and ventilation conditions until the mycelium covers the plate, then marking the medium with multiple parallel horizontal and vertical lines, adding glycerol aqueous solution to adjust the surface water activity to the range of 0.93–0.97, and applying a controlled spectral induction using alternating blue light and UV-A. Finally, a high-concentration spore suspension is obtained through elution. This invention significantly improves the sporulation yield and spore germination rate per plate while significantly reducing batch-to-batch variation. It is suitable for constructing standardized artificial infection models of *Anthracnose citrinum*, and can be used for pesticide efficacy evaluation, disease-resistant variety screening, and fruit storage and preservation research.

The invention belongs to the technical field of phytopathology and agricultural microorganisms, and particularly relates to a method for promoting efficient spore production of colletotrichum gloeosporioides, an obtained colletotrichum gloeosporioides spore suspension and application of the colletotrichum gloeosporioides spore suspension. The method comprises the following steps: inoculating a high-concentration carrot agar culture medium with a colletotrichum gloeosporioides cake, culturing in a spore induction cavity with controllable temperature, humidity and ventilation conditions until hyphae cover a flat plate, carrying out grid scribing according to a plurality of horizontal and vertical parallel lines, dropwise adding a glycerol aqueous solution to adjust the surface water activity of the culture medium to be 0.93-0.97, and culturing at the temperature of 20-30 DEG C to obtain the colletotrichum gloeosporioides. Controllable spectrum induction with blue light and UV-A alternating combination is applied, and finally high-concentration spore suspension is obtained through elution. According to the method, the single-plate sporulation quantity and the spore germination rate are remarkably improved, meanwhile, the batch-to-batch variation is remarkably reduced, and the method is suitable for constructing a standardized artificial infection model of the fruit anthracnose and is used for pesticide effect evaluation, disease-resistant variety screening and related research of fruit storage and preservation.