The invention relates to a
jerusalem artichoke blade phenolic extract, as well a preparation method and application of the
jerusalem artichoke blade phenolic extract. The preparation method comprises the following steps of:
drying fresh
jerusalem artichoke blades, and crushing the fresh jerusalem artichoke blades to obtain
dry powder of the jerusalem artichoke blades; putting the
dry powder of the jerusalem artichoke blades into a decompression
reflux extraction device, adding de-ionized water, extracting at
room temperature to obtain a water phenolic extract, and concentrating or
drying the water phenolic extract; or, adding an
organic solvent into the decompression
reflux extraction device, extracting while refluxing or decompressing and refluxing, and concentrating or
drying to obtain an
organic solvent phenolic extract; and extracting the water phenolic extract of the jerusalem artichoke blades or the
organic solvent phenolic extract by using
petroleum ether,
chloroform,
ethyl acetate and n-butyl
alcohol respectively in turn according to the volume ratio in a stepwise manner, and finally, concentrating and drying to obtain the stepwise phenolic extract. The extract disclosed by the invention belongs to the novel
plant-source (vegetative)
pesticide, has specially good effects on a plurality of
plant pathogenic bacteria, such as
botrytis cinerea,
phytophthora capsici leonian,
colletotrichum gloeosporioides,
rhizoctonia cerealis and gaeumannomyces graminis, and also can be used as a natural
plant-source
fungicide and a preservation
preservative of
fruits and vegetables.