216 results about "Colletotrichum gloeosporioides" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

The invention discloses bacillus methylotrophicus F7 and an application thereof. The invention aims at providing a strain which has a plurality of beneficial properties, namely producing siderophore, cellulase and protease, producing auxin, producing ammonia and the like; the strain not only can be used for effectively inhibiting various pathogenic bacteria such as ralstonia solanacearum, fusarium oxysporum f.sp.cubense, colletotrichum gloeosporioides, fusarium oxysporum f.sp cucumerinum but also can promote plant growth. In accordance with the technical scheme, the bacillus methylotrophicus F7 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 1, 2016, with preservation number of CGMCC No.12124; in addition, five surfactin homologs, six fengycin homologs and three iturin homologs, which have an antibacterial effect, are extracted from fermentation liquid of the bacillus methylotrophicus F7; and the bacillus methylotrophicus F7 belongs to the field of agricultural biotechnology.

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu / g on the solid fermentation medium of wheat bran and corncob nutrient solution.

The invention discloses LAMP (loop-mediated isothermal amplification) primer composition for detecting colletotrichum gloeosporioides and an application of the LAMP primer composition. A GS (glutamine synthetase) gene is used as a target gene, primers which have high specificity and high sensitivity and can be used for LAMP detection of the colletotrichum gloeosporioides are designed and screened out, and the LAMP primer composition consists of a forward outer primer GS-F3 shown in SEQ ID NO.2, a reverse outer primer GS-B3 shown in SEQ ID NO.3, a forward inner primer GS-FIP shown in SEQ ID NO.4, a reverse inner primer GS-BIP shown in SEQ ID NO.5, a loop primer GS-LF shown in SEQ ID NO.6 and a loop primer GS-LB shown in SEQ ID NO.7. The primer composition is mainly used for quickly detecting the colletotrichum gloeosporioides, and only 2 hours are spent on detection each time.

The invention relates to a primer for detecting orchid colletotrichum gloeosporioides molecules and a using method of the primer, which is specially used for detecting orchid colletotrichum gloeosporioides specific molecules and belongs to the fields of detection, identification and prevention and treatment of crop diseases. A pair of specific primers of orchid colletotrichum gloeosporioides comprising an upstream primer P1:5'-GGCCTCCCGCCTCCGGGCGGGTC-3' and a downstream primer P2:5'-TGAGGGCCTACATCAGCT-3' are subjected to polymerase chain reaction (PCR) amplification and agarose gel electrophoresis and can specifically amplify a specific amplification product with the segment length of 304bp in a plant infected with orchid colletotrichum gloeosporioides with pure DNA and germs and a culture medium. The specific molecule detection primer and the using method thereof can be used for detecting the orchid colletotrichum gloeosporioides in the plant infected with the orchid colletotrichum gloeosporioides and the culture medium quickly, sensitively and specifically, can also be used for performing early diagnosis on field diseases and monitoring and identifying germs, and provides reliable technological and theoretical basis for preventing and treating diseases caused by the orchid colletotrichum gloeosporioides.

The invention discloses a Trichoderma asperellum TC01. The Trichoderma asperellum TC01 is collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC NO. 3.19218. The Trichoderma asperellum TC01 has the remarkable inhibiting effect on a tea-tree anthracnose pathogenic bacterium Colletotrichum gloeosporioides and an apple valsa canker pathogenic bacterium Phytophthora plurivora, and antibacterial substances generated by the Trichoderma asperellum TC01 can also inhibit growth of the tea-tree anthracnose pathogenic bacterium and the apple valsa canker pathogenic bacterium. The trichoderma asperellum can also promote growth of tea seedlings, and is a strain having the good environmental safety, the biological control capacity and the growth promotion function.

The invention relates to bacillus velezensis having various disease preventive effects, application thereof and a biocontrol inoculant and belongs to the technical field of plant disease and pest prevention and control. Bacillus velezensis MS012 having bacteriostatic activity is obtained by separating and screening from healthy branches of malus sieversii. Bacillus amyloliquefaciens has remarkableantagonistic effect on botryosphaeria dothidea, colletotrichum gloeosporioides, phomopsis mali, rhizoctonia cerealis, gaeumannomyces graminis, botrytis cinerea, fusarium graminerum, clivia stem basaldecay pathogen, nandina anthrax pathogen and erwinia amylovora and has broad spectra in bacteriostatic object, and a new microbial resource is provided for biocontrol on disease and pest of plants like apple trees.

The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.

The invention discloses antibacterial and anti-virus applications of marine bacillus amyloliquefaciens 1A in fruit postpartum pathogenic fungi. The fruit postpartum pathogenic fungi are colletotrichum gloeosporioides, coniella diplodiella, pear alternaria alternata and penicillium italicum. The separating method of the marine bacillus amyloliquefaciens 1A comprises the following steps: collecting seawater, sea mud, marine debris, clam, enteromorpha and mudskipper samples in Lianyungang sea area; carrying out separation and purification of bacteria after enriching, so as to obtain a plurality of pure strains of marine bacteria; determining the inhibiting effects of the pure strains of the marine bacteria on the four fruit postpartum pathogenic fungi for a test by using a plate confrontation method; screening to obtain the strains of which the width of the bacteriostatic band is greater than 5mm; determining the bacteriostatic action of sterile fermented liquid on the four fruit postpartum pathogenic fungi by using an oxford cup method; and screening to obtain the bacillus amyloliquefaciens 1A of which the width of the bacteriostatic band is greater than 10mm. Therefore, the marine bacillus amyloliquefaciens 1A has a broad application prospect.

The invention belongs to the technical fields of medicinal plants and agricultural microorganism and particularly relates to an endophytic fungi which is separated from a medicinal plant huperzia serrata, can generate huperzine a or analogues, and can be used in mass production of the huperzine a or analogues. The separated endophytic fungi ES026 is identified as colletotrichum gloeosporioides bymolecular biology and morphology, and the strain has been preserved in China Center For Type Culture Collection (CCTCC), with the preservation number of CCTCC NO: M2011046. The endophytic fungi is significantly characterized in that after liquid fermentation, the strain can generate an active substance with reversibility which can inhibit acetylcholinesterase, and high-performance liquid chromatography and mass spectrum analysis shows that the compound is huperzine a (HupA), thus a new medicinal herb source for treating alzheimer's disease is provided.

The invention discloses pseudomonas aeruginosa JT86 and an application of the pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose. The pseudomonas aeruginosa JT86 is screened fromsoil, and the strain is collected in the Guangdong Microbiological Culture Collection Center on September 30, 2019, and has a collection number of GDMCC NO:60799. The strain and a volatile matter thereof can completely inhibit sclerotium germination of rhizoctonia solani, sclerotium rolfsii and sclerotinia sclerotiorum and inhibit growth of sclerotium hyphae, so that the sclerotium hyphae have flakes and wrinkles; the strain also has a good inhibition effect on the rhizoctonia solani, the sclerotium rolfsii, the sclerotinia sclerotiorum, magnaporthe grisea, fusarium oxysporum f. sp. cubense,brassica parachinensis anthracnose, colletotrichum gloeosporioides and colletotrichum capsici; and therefore, the strain can treat recalcitrant soil-borne pathogenic bacteria in soil in a targeted manner, effectively inhibits the sclerotiniose, and has a good application prospect in preparation of a soil treatment agent for preventing and treating soil-borne fungal diseases.

The invention belongs to the field of biocontrol strain excavation and utilization, in particular to bacillus subtilis Baisha2C for restraining plant pathogenic fungi, and the preservation number of the bacillus subtilis Baisha2C is CGMCC No.10963. The invention further discloses molecular biological characteristics, physiological-biochemical characteristics and a cultivation method of the bacillus subtilis Baisha2C, and the bacillus subtilis Baisha2C has activity for restraining various kinds of the plant pathogenic fungi, such as fusarium colletotrichum, colletotrichum gloeosporioides and fusarium.

The invention discloses a trichoderma harzianum strain and application thereof. The trichoderma strain is a trichoderma harzianum strain Hb13-3-2 CGMCC No. 13163. The strain and metabolites thereof have broad-spectrum activities of inhibiting pathogenic fungi. The contrast growth experiment proves that the trichoderma harzianum strain has the capacity of resisting red root pathogenic bacteria of rubber trees, colletotrichum gloeosporioides and other pathogenic bacteria, and further has the effects of controlling multiple other pollution microbes and promoting growth of rubber seedlings. Therefore, the trichoderma harzianum strain can be immediately applied to the cultivation process of the rubber trees at the seedling stage, healthy growth of the rubber seedlings is promoted, and occurrence of rubber tree root diseases can be shielded or inhibited.

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.

The present invention discloses a chestnut preservation method and belongs to the technical field of chestnut preservations. The preservation method consists of the following steps: (1) fruit selecting: insect pest free, full and harmless chestnuts are selected; (2) preservative treating: the selected chestnuts are soaked with the preservative for 15-35 minutes; (3) clean water soaking: the treated chestnuts in the step (2) are soaked with the clean water at 40 to 50 DEG C for 20-40 minutes; (4) biological treating: the treated chestnuts in the step (3) are soaked with water solution containing endogenetic bacteria for 15-25 minutes, and the soaked chestnuts are air-dried; (5) water content controlling: the treated chestnuts in the step (4) are freeze-dried or cold-air dried to control the moisture content to be 38-44%; and (6) refrigerating: the treated chestnut in the step (5) are put into a cold storage to be preserved at a humidity of 85-95% and a temperature of -4 to 0 DEG C. The endogenetic bacteria are endogenetic bacteria separated from the chestnuts to be preserved and at least have both obvious inhibitions on fusarium solani and colletotrichum gloeosporioides.

The invention discloses an application of pseudolaric acid B in preventing plant diseases. An experiment proves that the pseudolaric acid B has agricultural fungicidal activity. The compound has good bacteriostasis on setosphaeria turcica, pyricularia grisea, botrytis cinerea, rhizoctonia solani, mango stem end rot pathogen and colletotrichum gloeosporioides when the concentration for testing is 5mg / L, and the EC value is between 0.08mg / L-1mg / L; the compound has a certain spore germination inhibition on the setosphaeria turcica and the botrytis cinerea when the EC values are only 7.806mg / L and 4.104mg / L respectively; and at the same time, the result of a living body experiment shows that the compound further has good protective and therapeutic actions on the botrytis cinerea. The result indicates that the pseudolaric acid B has broad-spectrum bactericidal effect on the plant diseases, which lays the foundation for creating a novel non-polluted plant source pesticide by using the unique plant pseudolaric acid B in China.

The invention discloses a polygonatum cyrtonema endophyte strain ZJU-C612-1, i.e. Bacillus velezensis ZJU-C612-1 with a preservation number of CCTCC NO: M 2018311. The invention also discloses the uses of the strain in inhibiting the growth of Colletotrichum gloeosporioides, Fusarium oxysporum, C. truncatum, F. graminearum, F. solami, Rhizoctonia. solani and F. oxyporum f. sp. Niveum and promotingthe growth of polygonatum kingianum tissue culture seedlings and polygonatum cyrtonema tubers.

Bacillus methylotrophicus, and preparation and applications of a microbial inoculum thereof are disclosed and belong to the technical field of agricultural microbes. The bacillus methylotrophicus HZ-9 is deposited in the China General Microbiological Culture Collection Center on April 13, 2017, and the accession number of the strain is CGMCC No.14022. The antibacterial spectrum of the strain is wide. The strain has strong inhibiting effects on pathogenic bacteria causing anthurium andraeanum bacterial leaf spot, and other pathogenic bacteria such as colletotrichum gloeosporioides, fusarium oxysporum, poplar rot pathogenic bacteria, rhizoctonia solani and botrytis cinerea. The inhibiting effects on the pathogenic bacteria causing anthurium andraeanum bacterial leaf spot are especially significant. The bacillus methylotrophicus can stably colonize anthurium andraeanum root, blades and petiole surfaces. The microbial inoculum prepared from the strain can be applied for preventing and controlling the anthurium andraeanum bacterial leaf spot and other diseases.

The invention provides a spore production method of colletotrichum gloeosporioides. The method comprises the following steps: step 1: inoculating colletotrichum gloeosporioides onto a medium, and performing light-dark alternate culture in a constant temperature incubator till the colletotrichum gloeosporioides almost grows all over the medium; step 2: adding a small amount of sterile water in a sterile operation platform, scraping surface aerial hyphae with a sterile glass rod and pouring out the surface aerial hyphae, covering a culture dish cover, and ensuring that a culture dish is upward to be irradiated under ultraviolet lights; step 3: then performing light-dark alternate culture again in the constant temperature incubator, so as to obtain a large quantity of spores. According to themethod, a large quantity of spores can be obtained within 12 to 20d, the sporulation quantity is 10 to 20 times that of not treated colletotrichum gloeosporioides, and a large quantity of spores canbe obtained in a short time.

The invention belongs to the technical field of biologics, and in particular discloses a persimmon tree colletotrichum gloeosporioide SSR (Simple Sequence Repeat) primer pair developed based on sibling species genome and application of the primer pair. According to the persimmon tree colletotrichum gloeosporioide SSR primer pair, 30 pairs of SSR primers are developed by finding SSR by using MISA software on the basis of the genome of a sibling species, namely, colletotrichum gloeosporioides, of persimmon tree colletotrichum gloeosporioides, and 15 pairs of polymorphism SSR primers are obtained by screening 6 different persimmon tree colletotrichum gloeosporioides; the persimmon tree colletotrichum gloeosporioide SSR primer pairs provided by the invention are novel markers which exist stably, can be used for analyzing genetic diversity and genetic differentiation on the persimmon tree colletotrichum gloeosporioides, can be also used for identifying varieties of the persimmon tree colletotrichum gloeosporioides and studying ties of consanguinity, and have very important significances in effective prevention and control on the persimmon tree colletotrichum gloeosporioides.

The invention discloses Streptomyces sp.B5017. The Streptomyces sp.B5017 is characterized in that the preservation number is CCTCC NO: M2019648, and the Streptomyces sp.B5017 is classified and named as Streptomyces sp.B5017. Tests show that the marine-derived streptomyces new strain disclosed by the invention has the effect of antagonizing various phytopathogens, and has an inhibiting effect on gloeosporium musarum, colletotrichum gloeosporioides, colletotrichum pearl plum, botryosphaeria dothidea, fusarium oxysporum f.sp.cubense race 4, fusarium oxysporum f.sp.cubense race 1 microspecies or alternaria alternata. Fermentation liquor prepared from the Streptomyces sp.B5017 disclosed by the invention can effectively avoid withered and yellow withering of leaves caused by the fusarium oxysporum f.sp.cubense race 4 and race 1 in potting experiments, which proves that the Streptomyces sp.B5017 is capable of effectively preventing and treating banana wilt and enhancing the disease resistanceof bananas, has good ecological benefits and social benefits, and has a good application prospect when being developed as a biocontrol agent.

The invention discloses a radix notoginseng mitogen-activated protein kinase kinase gene PnMAPKK1.A nucleotide sequence of the gene PnMAPKK1 is as shown in SEQ ID NO:1, and the gene PnMAPKK1 codes mitogen-activated protein kinase kinase.According to functional genomics related technical researches, the gene PnMAPKK1 has a function of improving pathogenic fungus resistance of plants.After the antifungal gene PnMAPKK1 is constructed to a plant expression vector and transferred into tobaccos to realize overexpression, transgenic tobacco plants have extremely high in-vitro antifungal activity.The transgenic tobacco plants with PnMAPKK1 overexpressed have an evident inhibition effect on growth of fusarium solani, colletotrichum gloeosporioides, verticillium fusarium and botryosphaeria.

The invention relates to Antarctic-derived Bacillus sp.N311 and an application of Bacillus sp.N311 for preventing and treating phytopathogenic fungi, which relate to Bacillus sp. A preservation number of the Bacillus sp.N311 is CCTCC NO: M 2015607. The Bacillus sp.N311 can be used for preparing a fermentation supernatant for preventing and treating phytopathogenic fungi. The phytopathogenic fungi comprise fusarium, gloeosporium musarum, Paecilomyces variotii, sclerotinia sclerotiorum, Alternaria longipes, Trichoderma viride, Rhizoctonia solani, alternaria alternate, botrytis cinerea and Colletotrichum gloeosporioides. The Bacillus sp.N311 has good biological prevention and treatment effects in Rape Sclerotinia Rot pot culture prevention and treatment tests, and prevention and treatment effects can reach more than 50%. The Bacillus sp.N311 fermentation supernatant has wide fungus-inhibiting spectrum and good biocontrol effect, has advantages of no toxicity, difficult generation of drug resistance, environment safety and biosafety, and has wide application prospect.

The invention discloses a primer and a detection method for real-time fluorescent quantitative PCR detection of colletotrichum gloeosporioides of a hevea brasiliensis. A specific primer is designed according to a ribosome ITS sequence of colletotrichum gloeosporioides of the hevea brasiliensis, quantitative analysis is performed on the colletotrichum gloeosporioides through real-time fluorescent quantitative PCR, and the change rule of DNA of the colletotrichum gloeosporioides in tissue after inoculation of leaves of the hevea brasiliensis along with time is determined. The hevea brasiliensiscolletotrichum gloeosporioides quantitative detection technology provided by the invention is high in specificity, high in sensitivity, high in repeatability, accurate in quantification, high in speedand capable of achieving a full-closed reaction, colletotrichum gloeosporioides is identified and can be accurately quantified, the thallus content of hevea brasiliensis leaves at different time points after thallus infection is obtained, and therefore, early warning and preventive measures are carried out when the disease does not appear, the loss caused by the disease is reduced to the minimum,and the method is of great significance to early diagnosis, disease prediction and comprehensive prevention and treatment of the disease.

The invention discloses a method for controlling strawberry anthracnose. The method comprises the following steps: (1) putting Chinese medicinal herbs into water for boiling, and carrying out filtration to obtain a Chinese medicinal herb extract and a filter residue; (2) mixing the filter residue and the Chinese medicinal herb extract with sulfur, copper sulfate, ferrocene aliette, fosetyl-aluminum and soil to obtain culturing soil; (4) planting to-be-planted seedlings in the culture soil for culturing. In the method, the Chinese medicinal herbs comprise mugwort leaves, angelica dahurica, calamus, cortex meliae, rheum officinale, caulis perllae, fructus cnidii, ephedra, urtica cannabina L., cassia twig and pomegranate rind. Through adoption of the method, the strawberry anthracnose can be effectively controlled, and the medicine-resistant (medicine-tolerant) property of strawberry colletotrichum gloeosporioides is relatively low.

The invention relates to a jerusalem artichoke blade phenolic extract, as well a preparation method and application of the jerusalem artichoke blade phenolic extract. The preparation method comprises the following steps of: drying fresh jerusalem artichoke blades, and crushing the fresh jerusalem artichoke blades to obtain dry powder of the jerusalem artichoke blades; putting the dry powder of the jerusalem artichoke blades into a decompression reflux extraction device, adding de-ionized water, extracting at room temperature to obtain a water phenolic extract, and concentrating or drying the water phenolic extract; or, adding an organic solvent into the decompression reflux extraction device, extracting while refluxing or decompressing and refluxing, and concentrating or drying to obtain an organic solvent phenolic extract; and extracting the water phenolic extract of the jerusalem artichoke blades or the organic solvent phenolic extract by using petroleum ether, chloroform, ethyl acetate and n-butyl alcohol respectively in turn according to the volume ratio in a stepwise manner, and finally, concentrating and drying to obtain the stepwise phenolic extract. The extract disclosed by the invention belongs to the novel plant-source (vegetative) pesticide, has specially good effects on a plurality of plant pathogenic bacteria, such as botrytis cinerea, phytophthora capsici leonian, colletotrichum gloeosporioides, rhizoctonia cerealis and gaeumannomyces graminis, and also can be used as a natural plant-source fungicide and a preservation preservative of fruits and vegetables.