Spore production method of colletotrichum gloeosporioides

A technology of glyospora anthracnose and spores, which is applied in the biological field and can solve problems such as increased work, unfavorable development, and less spore production

Inactive Publication Date: 2018-05-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the spore research experiment and spore inoculation experiment of glyospora anthracnose, it is often necessary to obtain a large number of spores in a short period of time, because it takes 15-60 ...

Method used

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  • Spore production method of colletotrichum gloeosporioides

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Experimental program
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Embodiment

[0023] Inoculate Gluespora anthracnose on PDA medium with a diameter of 9cm, and culture it in a constant temperature incubator at 28°C with 12 / 12 light-dark alternate culture for 5 days until the medium is nearly full, add a small amount of sterile water to the sterile operating table , use a sterile glass rod to scrape off the aerial hyphae on the surface, cover the petri dish, irradiate the side facing the mycelium with ultraviolet light for 12 hours, and then culture in a constant temperature incubator at 24°C with 12 / 12 light-dark alternate cultivation for 7~ After 15 days, a large number of spores can be obtained.

[0024] Such as figure 1 As shown, the left side is the spore production situation of G. anthracnose glyospora growing for 60 days without treatment; figure 1 Yes, a large number of spores can be obtained in a short time by adopting the method of the present invention, thereby providing convenience for the experiment, and reducing a large amount of preparatio...

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Abstract

The invention provides a spore production method of colletotrichum gloeosporioides. The method comprises the following steps: step 1: inoculating colletotrichum gloeosporioides onto a medium, and performing light-dark alternate culture in a constant temperature incubator till the colletotrichum gloeosporioides almost grows all over the medium; step 2: adding a small amount of sterile water in a sterile operation platform, scraping surface aerial hyphae with a sterile glass rod and pouring out the surface aerial hyphae, covering a culture dish cover, and ensuring that a culture dish is upward to be irradiated under ultraviolet lights; step 3: then performing light-dark alternate culture again in the constant temperature incubator, so as to obtain a large quantity of spores. According to themethod, a large quantity of spores can be obtained within 12 to 20d, the sporulation quantity is 10 to 20 times that of not treated colletotrichum gloeosporioides, and a large quantity of spores canbe obtained in a short time.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a spore-producing method of gliospora anthracis. Background technique [0002] Walnut anthracnose has the characteristics of long incubation period, short onset time, and strong explosiveness. It can cause fruit necrosis and fruit drop, leaves scorched and fall off, and also harm young shoots, seriously affecting walnut yield and quality, and causing serious economic losses. a catastrophic disease. Most scholars in my country believe that the asexual generation of the walnut anthracnose pathogen belongs to the half-know fungus, Colletotrichum Corda (C. gloeosporioides Penz). [0003] In the spore research experiment and spore inoculation experiment of glyospora anthracnose, it is often necessary to obtain a large number of spores in a short period of time, because it takes 15-60 days for the gloeosporin anthracnose to start spore production under the growth condi...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N3/00C12N1/14
Inventor 万雪琴曹广黎蒋时姣黄金亮衣建民尹焕焕何丹
Owner SICHUAN AGRI UNIV
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