Spore production method of colletotrichum gloeosporioides
A technology of glyospora anthracnose and spores, which is applied in the biological field and can solve problems such as increased work, unfavorable development, and less spore production
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[0023] Inoculate Gluespora anthracnose on PDA medium with a diameter of 9cm, and culture it in a constant temperature incubator at 28°C with 12 / 12 light-dark alternate culture for 5 days until the medium is nearly full, add a small amount of sterile water to the sterile operating table , use a sterile glass rod to scrape off the aerial hyphae on the surface, cover the petri dish, irradiate the side facing the mycelium with ultraviolet light for 12 hours, and then culture in a constant temperature incubator at 24°C with 12 / 12 light-dark alternate cultivation for 7~ After 15 days, a large number of spores can be obtained.
[0024] Such as figure 1 As shown, the left side is the spore production situation of G. anthracnose glyospora growing for 60 days without treatment; figure 1 Yes, a large number of spores can be obtained in a short time by adopting the method of the present invention, thereby providing convenience for the experiment, and reducing a large amount of preparatio...
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