161results about "Spore processes" patented technology

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The application discloses a Penicillium oxalicum strain, a biocontrol agent and application. A fungus is isolated and screened from soil, and the fungus is identified as Penicillium oxalicum by combining morphological characteristics and molecular identification, and is named as Penicillium oxalicum PO14 with a preservation number of CGMCC No.22461. The Penicillium oxalicum PO14 has a strong inhibitory effect on Fusarium fujikuroi spore germination and mycelium growth, and can be used for prevention and treatment of rice bakanae disease. The Penicillium oxalicum PO14 strain can be used for development of a biocontrol agent or a biological pesticide, and has a good application prospect in biological prevention of rice bakanae disease.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

A modified sporulating bacteria may comprise a nucleic acid cassette having a silent promotor that inhibits transcription by the modified sporulating bacteria. The nucleic acid cassette may include a nucleic acid sequence including a gene of interest that encodes a protein of interest. The nucleic acid cassette may include a nucleic acid sequence encoding a loading peptide that incorporates into a coat of a spore. The modified sporulating bacteria may comprise a pre-spore having the nucleic acid sequence having the gene of interest that encodes the protein of interest. A modified spore may be formed comprising a spore body containing the nucleic acid cassette, a spore coat, and a protein of interest derived from the gene of interest inserted into the spore coat made by exogenous cell-free biosynthesis components.

The invention belongs to the technical field of exosome preparation, and discloses a plant exosome with an anti-acne effect and a preparation method and application of the plant exosome with the anti-acne effect. According to the application, an integrated technical route of microbial regulation and induction, complexing-agent-free green extraction and synergistic acne resistance is established: a microbial strain spore suspension is used as a biological inducer, a plant active precursor is released by dynamic countercurrent of ethanol, and PEG / CaCl2 residues are completely eradicated through physical separation in the whole process; finally, the microorganism-induced plant exosome with high yield, high activity and high safety is obtained.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention relates to an actinomycete preparation and application thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect fecula. When the actinomycetes preparation is stored for five months, half a year or one year or more, the content of the actinomycetes in the actinomycetes preparation is 1 * 10 < 11 > CFU / g or more.

The invention relates to the technical field of fungus culture, and discloses a culture medium for inducing Stemphylium solani to produce spores, a preparation method and application, the preparation raw materials of the culture medium comprise a tomato extract, a carrot extract and agar, the total mass of the tomato extract and the carrot extract is 50g / L to 200g / L, and the content of the tomato extract is not less than 50g / L. According to the culture medium for inducing the Stemphylium solani to produce the spores, the preparation method and the application, the tomato extract and the carrot extract in a specific ratio are combined, so that optimal nutrition and environmental conditions are provided for the Stemphylium solani to produce the spores. Experimental data show that the sporulation quantity of the culture medium is remarkably higher than that of a conventional culture medium (such as CA, V8, PDA and the like), the technical problem that an existing solid culture medium is unstable in sporulation or does not produce sporulation is solved, the selected core raw materials tomato and carrot are common fruits and vegetables, low in cost and easy to obtain, expensive or special chemical reagents are avoided, and the production cost is reduced. And the research and development and application cost is greatly reduced.

The invention relates to the technical field of ecological restoration, and discloses an integrated toughness ecological restoration and reinforcement method for an expansive soil side slope, which sequentially comprises a deep crack restoration layer (1), a shallow barrier layer (2) and a surface ecological layer (3) from inside to outside in the depth direction of the expansive soil side slope, and the three layers form a protection system; from the overall view of the scheme, the fundamental problem of slope structure stability is solved through deep reinforcement, the reason that expansive soil swells when encountering water is cut off through shallow water blocking, long-term ecological restoration and surface protection are achieved through surface ecology, and full-dimensional toughness protection of deep stability control, shallow water control and surface ecology control is formed through a three-layer system. And finally, the core problems that in traditional expansive soil slope restoration, the short-term effect is obvious, but the long-term effect is prone to losing efficacy, and structural reinforcement and ecological restoration are difficult to consider at the same time are solved, and the double sustainable targets of slope safety stability and ecological environment restoration are achieved.

This invention relates to a method for germinating bacterial spores SP. In this method, a surface 5 is contacted with bacterial spores SP that were pretreated with at least one biosurfactant SBio and / or are applied in mixture with at least one biosurfactant SBio. SBio is a glycolipid, which is preferably selected from glucolipids, rhamnolipids, sophorolipids. The present invention also relates to the use of at least one biosurfactant SBio, wherein biosurfactant SBio is a glycolipid, for dispersing bacterial spores SP. The bacterial spores SP are preferably selected from the group consisting of Bacillus spores, Priestia spores. The method according to the invention may advantageously be used in a method of cleaning an object O or the human or animal body B.

The invention discloses a microbiological degraded Betallop simulation sample and a preparation method thereof, and belongs to the technical field of cultural relic degradation simulation. The method comprises the following steps: inoculating a specific decay-causing fungus suspension on the surface of a bay leaf simulated substrate for microbial degradation, wherein the specific decay-causing fungi comprise penicillium chrysogenum and humicola brownish black. The penicillium chrysogenum and the humicola brownish black obtained through screening have important values for researching a biological degradation mechanism of the cultural relics and developing a targeted biological prevention and control technology, and the simulation sample prepared by the method provides a standardized sample for protecting and repairing the Betashes; the method can be used for safely and efficiently verifying various reinforcing materials, mildew preventive, cleaning agent, protection process and repair technology and researching the aging mechanism, greatly reduces the risk of directly testing on precious cultural relics, and has important significance on promoting the scientific protection of plant fiber cultural relics such as Beijing.

The application provides a simple solid high-density fermentation method of streptomyces. The method comprises the following steps: mixing bran and scarab beetle dung at a mass ratio of 1-2:1 to obtain a fermentation substrate, adjusting the initial water content of the fermentation substrate to 70%, inoculating 1×10 7 -1×10 8 cfu / mL actinomycete spore suspension, and culturing at 28 DEG C for 4-8 days to obtain high-density spores. The application explores the influence of the formula, ratio, water content of the fermentation substrate, spore inoculation amount, fermentation temperature and fermentation time on the fermentation amount of actinomycete P8-2 spores. The experimental results show that under the optimal conditions, the spore number of P8-2 in the solid fermentation substrate is 2.48×10 13 cfu / g after 8 days of fermentation, which is significantly higher than that of the existing fermentation method. Moreover, the fermentation substrate of the application is made of bran and scarab beetle dung with a specific ratio, the raw materials are easy to obtain, the operation is simple, and the industrialized production is facilitated.

The invention discloses a preparation method of a co-culture metabolite carbon-based biostimulant based on different combinations of trichoderma, bacillus and metarhizium anisopliae. Compared with a single microorganism culture metabolite carbon-based preparation, the metabolite carbon-based preparation co-cultured by different combined micro-substances has obvious synergism in the aspects of wheat growth promotion and stress resistance improvement; the optimal proportion and time of the metabolite co-cultured by different combinations of the trichoderma, the bacillus velezensis and the metarhizium anisopliae adsorbed by the rice straw biochar are obvious in synergistic interaction in promoting wheat seed germination and seedling growth and improving wheat stress resistance. Different combinations of co-culture metabolites and biochar compounded preparations can be developed into novel carbon-based biostimulant, and the carbon-based biostimulant has important significance in promoting growth of wheat and corn, improving stress resistance and disease resistance and the like.

The present application relates to the technical field of plant root rot prevention, and particularly relates to preparation and application of a Trichoderma granule for preventing and treating root rot of Lycium barbarum L. 9 The present application relates to the technical field of plant root rot prevention, and particularly relates to preparation and application of a Trichoderma granule for preventing and treating root rot of Lycium barbarum L. The granule comprises a fermentation product of a Trichoderma ZATA02 strain, an organic fermentation substrate, a mineral filler and a binder; the Trichoderma ZATA02 strain has been preserved, and the effective viable spore number in the granule is not less than 5x10 9 CFU / g, and the preparation method comprises the following steps: after the ZATA02 strain is fermented in an optimized liquid medium, the strain is inoculated into a solid substrate compounded by wheat bran and rice husk for fermentation, the obtained solid fermentation product is mixed with kaolin, granulated with a sodium carboxymethyl cellulose solution as a binder, and dried to obtain the granule. The granule prepared by the synergistic effect of the specific strain, the optimized fermentation process and the stable dosage form has high viable spore number, good storage stability, excellent prevention and treatment effect on root rot of Lycium barbarum L. in the field, and has the advantages of high efficiency, greenness and convenience.

The invention discloses a method for simply and efficiently promoting mycelial growth and non-co-spore production of AM (arbuscular mycorrhizal) fungi and application of the method. According to the method, a dual-cultured AM fungus fungicide is refrigerated and preserved for a long time, and the preservation time is 3-38 months. The spores obtained after long-term preservation are inoculated on a culture medium under a non-symbiotic condition, and C14 fatty acid is added into the culture medium. After C14 fatty acid is exogenously added into the spores refrigerated for a long time, the amount of hyphae and the production amount of the spores formed by AM fungus non-symbiosis can be remarkably increased, and the density of the hyphae is 7.3 times that of the hyphae without the fatty acid; each mother spore can form about 54 spores, and the yield of the spores is about 10 times that of the non-symbiotic spores treated by adding C14 fatty acid in combination with an exogenous stimulant. According to the method, the AM fungus fungicide can be propagated under the non-symbiotic condition, the propagation efficiency is high, the non-symbiotic sporulation quantity of AM fungi can be remarkably increased, operation is easy and convenient, the cost is low, the period is short, and wide application and popularization prospects are achieved.

The invention relates to the technical field of yoghurt food, in particular to ganoderma lucidum fermentation liquor as well as a preparation method and application thereof. According to the preparation method provided by the invention, the preparation efficiency can be effectively improved, the content of ganoderan and triterpenoid components in the ganoderma lucidum fermentation liquor is remarkably increased, and large-scale production is facilitated. Metabolite produced in the preparation process of the ganoderma lucidum fermentation liquor can endow the yoghourt with unique flavor. The ganoderma lucidum fermentation liquor provided by the invention is combined with the yoghourt, so that the nutritional ingredients of the yoghourt can be enriched, and the nutritional value and the health-care function of the yoghourt can also be improved. According to the set yoghurt prepared by taking the ganoderma lucidum fermentation liquor and the milk as main raw materials, when the addition amount of the ganoderma lucidum fermentation liquor is 10wt% of that of the milk, the oxidation resistance, the acidity, the water-holding capacity and the sensory evaluation of the set yoghurt are optimal.

The application discloses a method for recycling waste liquid in the production process of Venetian Fusarium protein, which comprises the steps of component analysis, nutrient supplement, fermentation verification and recycling evaluation, and aims at the problems of high water consumption and high emission in the preparation process of microbial protein of the filamentous fungus Venetian Fusarium, and builds component analysis, resource utilization potential evaluation and recycling process demonstration of two types of typical process waste liquid. Through the hierarchical recycling strategy design of setting the fermenting waste liquid to be reused once and the de-nucleic acid waste liquid to be reused twice, the theoretical production amount of the two kinds of waste water is reduced from 18.8 t to 7.7 t under the condition of four production cycles, and the emission reduction ratio is about 59.04%, which shows that the method has significant water saving and emission reduction potential in theory, and can provide data support and technical basis for the optimization of the green manufacturing process of the filamentous fungus protein.

The invention discloses a solid-state fermentation method of penicillium oxalicum, a penicillium oxalicum complex microbial inoculant and application of the penicillium oxalicum complex microbial inoculant in promoting colonization of the penicillium oxalicum in soil and improving available phosphorus in the soil. The solid-state fermentation method comprises the following steps: mixing a penicillium oxalicum spore suspension and a fermentation substrate for fermentation at 30 + / -0.5 DEG C; the fermentation substrate comprises waste flammulina velutipes residues and wheat bran in a mass ratio of 1: 1. The Penicillium oxalicum complex microbial inoculant comprises the following components in parts by mass: 10-25 parts of a Penicillium oxalicum solid-state fermentation product obtained by a solid-state fermentation method, 20-25 parts of diatomite, 20-30 parts of zeolite powder, 5-10 parts of potassium fulvate and 10-15 parts of humic acid.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

The invention belongs to the technical field of mushroom breeding, and particularly discloses a novel mushroom strain Xiangxiang No.1 as well as application and a cultivation method of the novel mushroom strain Xiangxiang No.1. The Xiangxiang No.1 strain is obtained by hybridizing and breeding 'Jiuxiang autumn 7' and 'autumn xiang 607' which are used as parents, and the preservation number of the Xiangxiang No.1 strain is CCTCC NO: M 20252774. Compared with conventional shiitake mushrooms, the shiitake mushroom Xiangxiang No.1 mushroom age is extremely short and is about 80 days, and the factory shiitake mushroom production requirements are met; the mushroom stick is small in fruiting number, heavy in single mushroom weight, beneficial to picking and high in commodity; sporocarp pilei are not unfolded under a low-temperature condition, pleats are not exposed, and spores cannot be collected; the pileus is slowly expanded under a high-temperature condition, the pleats are exposed, the spores can be collected, but the spores cannot germinate, and the method has a relatively high scientific research value in the sexual propagation research of the shiitake mushrooms.

The present invention relates to an actinomycete preparation and the use thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect frass. Upon storage for a period of five months, six months, or one year or longer, the content of the actinomycetes in the actinomycete preparation is 1×1011 CFU / g or more.

The invention discloses Mortierella elongata and application of the Mortierella elongata, the strain number of the Mortierella elongata is JSAFC 247, the Mortierella elongata is preserved in the China General Microbiological Culture Collection Center on December 9, 2024, and the preservation number is CGMCC No.41703. The Mortierella elongata has the advantages that the Mortierella elongata can be used for preparing the Mortierella elongata and the application of the Mortierella elongata; the mortierella elongata strain provided by the invention is strong in pathogenicity to larvae of the larvae of the corpulenta aeruginosa, is environment-friendly and pollution-free, is not easy to generate drug resistance, can be used as a living body biopesticide, and has very strong application potential in biological prevention and control of the corpulenta aeruginosa.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention discloses a microbial acidic soil conditioner, and belongs to the technical field of soil improvement. The microbial acid conditioner comprises trichoderma virens T259 with the preservation number of CGMCC No.41109. Through the synergistic interaction of the trichoderma virens T259, the calcium magnesium phosphate fertilizer, the organic fertilizer, the fulvic acid and the chitosan, the acid-base property of soil can be adjusted, and the microbial structure of the soil can be improved; the composition can also induce crops to generate resistance and promote crop growth, so that resistance and development to diseases are enhanced, and the yield and quality are improved.

The invention provides a method for elevated propagation of AM (arbuscular mycorrhizal fungi) fungal spores, which comprises the following steps: (1) selecting arbuscular mycorrhizal fungi as an inoculant, and taking the spores of the arbuscular mycorrhizal fungi for later use; (2) selecting a proper culture medium, and sterilizing for later use; (3) before planting, sterilizing the A-shaped cultivation frame, washing the A-shaped cultivation frame with purified water, and after the cultivation tank is drained, putting the prepared substrate into the cultivation tank; (4) pretreating the Chinese chive roots, and fermenting the cut Chinese chive roots in purified water added with rooting powder; (5) leveling the substrate placed in the cultivation groove, forming a planting ditch, uniformly scattering the prepared spores into the ditch, and uniformly mixing the spores with the substrate; then, the fermented leek roots are transplanted into a substrate. The method has the advantages that the space utilization rate is increased by adopting A-shaped cultivation, the propagation period is shortened by taking Chinese chives as a host, the interference of infectious microbes is reduced and symbiosis is promoted by matching with a matrix, the method is adaptive to various AM fungi, and the spore yield and stability are superior to those of a traditional method.

The present invention relates to Saccharomyces cerevisiae yeast ascospores in dry or suspension form, for use as a medicament, and in particular for use as an anti-inflammatory agent, antiviral agent or anti-tumour agent. The present invention also relates to Saccharomyces cerevisiae yeast ascospores characterized in that they are in dry form and in that they have a solids content ranging from 95% to 99.9%. The invention further relates to a method for preparing the Saccharomyces cerevisiae yeast ascospores in dry or suspension form. The method for preparing the ascospores in dry form consists in subjecting to drying a suspension of ascospores having a solids content ranging from 1.5% to 5%, said drying being carried out using a fluidized bed or by freeze-drying.