256results about "Spore processes" patented technology

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses Mucor irregularia and application thereof, the strain number of the Mucor irregularia is JSAFC 3408, the Mucor irregularia is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC No.41982. The Mucor irregularia has the advantages that the Mucor irregularia has the advantages that the Mucor irregularia can be used for preparing the Mucor irregularia; according to the application, the soil around the pepper is separated and purified, so that the Mucor irregularia is obtained. The strain can inhibit the growth of the huanghuai glossospora, promote the development of the pepper and regulate the growth of plants, and has great significance for the development of a biocontrol fungicide for preventing and treating the pepper root rot in the future.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention belongs to the technical field of building materials, and particularly relates to a preparation method of alkali-treated polypropylene fiber and recycled aggregate loaded bacillus coriolis synergistic microbial concrete. According to the method, a polypropylene fiber reinforced concrete matrix is subjected to alkali treatment, calcium carbonate is generated by combining the mineralization effect of recycled aggregate loaded microorganisms, and efficient crack repair and mechanical property improvement are achieved. The method specifically comprises the steps of microbiological treatment and loading, fiber surface modification and optimization, self-healing concrete preparation and the like. The recycled aggregate is used for fixing microorganisms and providing a mineralization environment, the interface bonding capacity is enhanced through alkali treatment fibers, and the calcium carbonate deposition efficiency is remarkably improved. The prepared self-healing concrete has excellent mechanical strength and compactness, and the repairing performance is also obviously improved. The method is simple in process and controllable in cost, the utilization of the recycled aggregate conforms to the concept of green building materials, and the method can be widely applied to preparation of microbial concrete and has remarkable environmental protection value and application prospects.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The application discloses a Penicillium oxalicum strain, a biocontrol agent and application. A fungus is isolated and screened from soil, and the fungus is identified as Penicillium oxalicum by combining morphological characteristics and molecular identification, and is named as Penicillium oxalicum PO14 with a preservation number of CGMCC No.22461. The Penicillium oxalicum PO14 has a strong inhibitory effect on Fusarium fujikuroi spore germination and mycelium growth, and can be used for prevention and treatment of rice bakanae disease. The Penicillium oxalicum PO14 strain can be used for development of a biocontrol agent or a biological pesticide, and has a good application prospect in biological prevention of rice bakanae disease.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

A modified sporulating bacteria may comprise a nucleic acid cassette having a silent promotor that inhibits transcription by the modified sporulating bacteria. The nucleic acid cassette may include a nucleic acid sequence including a gene of interest that encodes a protein of interest. The nucleic acid cassette may include a nucleic acid sequence encoding a loading peptide that incorporates into a coat of a spore. The modified sporulating bacteria may comprise a pre-spore having the nucleic acid sequence having the gene of interest that encodes the protein of interest. A modified spore may be formed comprising a spore body containing the nucleic acid cassette, a spore coat, and a protein of interest derived from the gene of interest inserted into the spore coat made by exogenous cell-free biosynthesis components.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention belongs to the technical field of exosome preparation, and discloses a plant exosome with an anti-acne effect and a preparation method and application of the plant exosome with the anti-acne effect. According to the application, an integrated technical route of microbial regulation and induction, complexing-agent-free green extraction and synergistic acne resistance is established: a microbial strain spore suspension is used as a biological inducer, a plant active precursor is released by dynamic countercurrent of ethanol, and PEG / CaCl2 residues are completely eradicated through physical separation in the whole process; finally, the microorganism-induced plant exosome with high yield, high activity and high safety is obtained.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The present invention relates to the use of aluminum chloride to increase the number of predatory organs produced by oligosporoid spores. This invention relates to the field of microorganisms, particularly the reproduction of microbial biocides. The present invention is the first to discover that aluminum chloride can increase the number of predatory organs produced by oligosporoid spores, thereby improving the predation efficiency of oligosporoid spores on nematodes, thereby reducing costs.

The invention relates to an actinomycete preparation and application thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect fecula. When the actinomycetes preparation is stored for five months, half a year or one year or more, the content of the actinomycetes in the actinomycetes preparation is 1 * 10 < 11 > CFU / g or more.

The invention relates to the technical field of fungus culture, and discloses a culture medium for inducing Stemphylium solani to produce spores, a preparation method and application, the preparation raw materials of the culture medium comprise a tomato extract, a carrot extract and agar, the total mass of the tomato extract and the carrot extract is 50g / L to 200g / L, and the content of the tomato extract is not less than 50g / L. According to the culture medium for inducing the Stemphylium solani to produce the spores, the preparation method and the application, the tomato extract and the carrot extract in a specific ratio are combined, so that optimal nutrition and environmental conditions are provided for the Stemphylium solani to produce the spores. Experimental data show that the sporulation quantity of the culture medium is remarkably higher than that of a conventional culture medium (such as CA, V8, PDA and the like), the technical problem that an existing solid culture medium is unstable in sporulation or does not produce sporulation is solved, the selected core raw materials tomato and carrot are common fruits and vegetables, low in cost and easy to obtain, expensive or special chemical reagents are avoided, and the production cost is reduced. And the research and development and application cost is greatly reduced.

The invention relates to the technical field of ecological restoration, and discloses an integrated toughness ecological restoration and reinforcement method for an expansive soil side slope, which sequentially comprises a deep crack restoration layer (1), a shallow barrier layer (2) and a surface ecological layer (3) from inside to outside in the depth direction of the expansive soil side slope, and the three layers form a protection system; from the overall view of the scheme, the fundamental problem of slope structure stability is solved through deep reinforcement, the reason that expansive soil swells when encountering water is cut off through shallow water blocking, long-term ecological restoration and surface protection are achieved through surface ecology, and full-dimensional toughness protection of deep stability control, shallow water control and surface ecology control is formed through a three-layer system. And finally, the core problems that in traditional expansive soil slope restoration, the short-term effect is obvious, but the long-term effect is prone to losing efficacy, and structural reinforcement and ecological restoration are difficult to consider at the same time are solved, and the double sustainable targets of slope safety stability and ecological environment restoration are achieved.

The invention belongs to the technical field of microorganism application, and particularly relates to application of Isaria endula 2018BY-1 in production of indoleacetic acid and promotion of plant growth, the amount of indoleacetic acid secreted in a liquid LB culture medium containing L-tryptophan is 226.25 + / -25.98 mu g / mL, and the Isaria endula 2018BY-1 can effectively increase the content of indoleacetic acid in a plant environment to promote plant growth. Specifically, main root cells are induced to divide, propagate and extend, and the height of plants is increased, so that the leaf area of the plants is increased, the crop quality is improved, and the yield is increased.

This invention relates to a method for germinating bacterial spores SP. In this method, a surface 5 is contacted with bacterial spores SP that were pretreated with at least one biosurfactant SBio and / or are applied in mixture with at least one biosurfactant SBio. SBio is a glycolipid, which is preferably selected from glucolipids, rhamnolipids, sophorolipids. The present invention also relates to the use of at least one biosurfactant SBio, wherein biosurfactant SBio is a glycolipid, for dispersing bacterial spores SP. The bacterial spores SP are preferably selected from the group consisting of Bacillus spores, Priestia spores. The method according to the invention may advantageously be used in a method of cleaning an object O or the human or animal body B.

The invention discloses a microbiological degraded Betallop simulation sample and a preparation method thereof, and belongs to the technical field of cultural relic degradation simulation. The method comprises the following steps: inoculating a specific decay-causing fungus suspension on the surface of a bay leaf simulated substrate for microbial degradation, wherein the specific decay-causing fungi comprise penicillium chrysogenum and humicola brownish black. The penicillium chrysogenum and the humicola brownish black obtained through screening have important values for researching a biological degradation mechanism of the cultural relics and developing a targeted biological prevention and control technology, and the simulation sample prepared by the method provides a standardized sample for protecting and repairing the Betashes; the method can be used for safely and efficiently verifying various reinforcing materials, mildew preventive, cleaning agent, protection process and repair technology and researching the aging mechanism, greatly reduces the risk of directly testing on precious cultural relics, and has important significance on promoting the scientific protection of plant fiber cultural relics such as Beijing.

The invention belongs to the technical field of microbial culture, and particularly relates to a fungus solid culture medium containing hermetia illucens components as well as a preparation method and application of the fungus solid culture medium. The culture medium is prepared from hermetia illucens powder, yeast powder, a carbon source, inorganic salt, agar and water. Wherein the hermetia illucens powder is a main nitrogen source. The preparation method comprises the following steps: dissolving the nutrient components, adding the agar, heating for dissolving, and sub-packaging. According to the invention, hermetia illucens powder is creatively used as a main nitrogen source, a natural host nutrition environment of insecticidal fungi is simulated, and the technical problems of slow mycelial growth and low sporulation quantity when the insecticidal fungi are cultured by a traditional culture medium are solved. Test results show that the culture medium provided by the invention significantly promotes growth of metarhizium anisopliae, the mycelial growth rate is nearly doubled compared with that of a traditional culture medium, the sporulation quantity is increased by 7-24 times, and a new technical scheme is provided for large-scale efficient production of insecticidal fungi.

The application provides a simple solid high-density fermentation method of streptomyces. The method comprises the following steps: mixing bran and scarab beetle dung at a mass ratio of 1-2:1 to obtain a fermentation substrate, adjusting the initial water content of the fermentation substrate to 70%, inoculating 1×10 7 -1×10 8 cfu / mL actinomycete spore suspension, and culturing at 28 DEG C for 4-8 days to obtain high-density spores. The application explores the influence of the formula, ratio, water content of the fermentation substrate, spore inoculation amount, fermentation temperature and fermentation time on the fermentation amount of actinomycete P8-2 spores. The experimental results show that under the optimal conditions, the spore number of P8-2 in the solid fermentation substrate is 2.48×10 13 cfu / g after 8 days of fermentation, which is significantly higher than that of the existing fermentation method. Moreover, the fermentation substrate of the application is made of bran and scarab beetle dung with a specific ratio, the raw materials are easy to obtain, the operation is simple, and the industrialized production is facilitated.

The invention discloses a preparation method of a co-culture metabolite carbon-based biostimulant based on different combinations of trichoderma, bacillus and metarhizium anisopliae. Compared with a single microorganism culture metabolite carbon-based preparation, the metabolite carbon-based preparation co-cultured by different combined micro-substances has obvious synergism in the aspects of wheat growth promotion and stress resistance improvement; the optimal proportion and time of the metabolite co-cultured by different combinations of the trichoderma, the bacillus velezensis and the metarhizium anisopliae adsorbed by the rice straw biochar are obvious in synergistic interaction in promoting wheat seed germination and seedling growth and improving wheat stress resistance. Different combinations of co-culture metabolites and biochar compounded preparations can be developed into novel carbon-based biostimulant, and the carbon-based biostimulant has important significance in promoting growth of wheat and corn, improving stress resistance and disease resistance and the like.

The present application relates to the technical field of plant root rot prevention, and particularly relates to preparation and application of a Trichoderma granule for preventing and treating root rot of Lycium barbarum L. 9 The present application relates to the technical field of plant root rot prevention, and particularly relates to preparation and application of a Trichoderma granule for preventing and treating root rot of Lycium barbarum L. The granule comprises a fermentation product of a Trichoderma ZATA02 strain, an organic fermentation substrate, a mineral filler and a binder; the Trichoderma ZATA02 strain has been preserved, and the effective viable spore number in the granule is not less than 5x10 9 CFU / g, and the preparation method comprises the following steps: after the ZATA02 strain is fermented in an optimized liquid medium, the strain is inoculated into a solid substrate compounded by wheat bran and rice husk for fermentation, the obtained solid fermentation product is mixed with kaolin, granulated with a sodium carboxymethyl cellulose solution as a binder, and dried to obtain the granule. The granule prepared by the synergistic effect of the specific strain, the optimized fermentation process and the stable dosage form has high viable spore number, good storage stability, excellent prevention and treatment effect on root rot of Lycium barbarum L. in the field, and has the advantages of high efficiency, greenness and convenience.

This invention belongs to the field of biotechnology, specifically disclosing a rapid sporulation method for *Alternaria ginseng*. The method involves activating and culturing *Alternaria ginseng* in PDA medium at 25°C in the dark for 10 days. Mycelial cakes are then collected from the colony edges using a punch. These mycelial cakes are inoculated onto a sporulation medium and cultured in a sealed dark environment at 22°C for 4 days. After making crisscross cuts 1 cm from the center of the colony, the cells are treated with ultraviolet light for 9 minutes. The culture dish is then left uncovered and placed at 22°C. Finally, the cells are cultured in a light incubator at 70% relative humidity for 12 hours followed by 12 hours in the dark, resulting in rapid sporulation at the cuts. Compared to existing technologies, this invention significantly shortens the sporulation cycle (5 days), greatly saving time and costs.