879results about "Spore processes" patented technology

The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and / or the generation of one or more tissue constructs from cell and / or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and / or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

The invention provides methods and related products for extracting nucleic acids such as DNA from prokaryotic spores. The invention also encompasses methods for identifying the source of such spores via analysis of the isolated nucleic acids.

The invention discloses an aspergillus flavus strain producing no aflatoxin. The aspergillus flavus strain producing no aflatoxin, provided by the invention, is specifically aspergillus flavus GZ-17; the preservation number of the aspergillus flavus strain in the general microbiology center of the China Committee for Culture Collection of Microorganisms is CGMCC No.8050. Experimental results show that the aspergillus flavus strain GZ-17 provided by the invention plays a role in restraining the poison production of the aspergillus flavus strain producing the aflatoxin; when the concentration ratio of the strain to a toxic producing fungus spore is 105: 105, the poison production restrained ratio of bacterium producing no poison to the bacterium producing poison is nearly 96%. The strain has important significance for restraining the infection of the aspergillus flavus producing poison to agricultural products and reducing the aflatoxin pollution in the agricultural products.

The invention relates to a biological preparation, in particular to a Trichodrema spp. microbial agent and a preparation method thereof. The method comprises the following steps: the dried and crushed 8-9 edible fungus chaff according to parts by weight is mixed with 2-4 auxiliary material according to parts by weight, the acidity-alkalinity pH of the mixed raw material is adjusted to 5.0-6.5, then the Trichodrema spp. suspension with 5-10 percent of mass ratio with the mixed raw material is inoculated to the mixed raw material and cultured for 7-10 days at the temperature of 8-30 DEG C, and after the Trichodrema spp. is generated, the well cultured material is dried and crushed to obtain Trichodrema spp. microbial agent. The invention takes the treated edible fungus chaff as the raw material to produce the Trichodrema spp. microbial agent, fully utilizes the rest nutritious substance in the edible fungus chaff as well as the characteristics of loose and permeability of the fungus chaff, promotes the value in use of the fungus chaff of the edible fungus, and simultaneously reduces the production cost of the Trichodrema spp. microbial agent, the produced Trichodrema spp. is mainly chlamydospore, the multiplication capacity and the colonizing capacity of the Trichodrema spp. in the microbial agent are enhanced, the capability of resisting the unfavorable environmental conditions is also enhanced and the survival time of the Trichodrema spp. is prolonged.

A strain of Trichoderma harzianum is obtained that is useful as a nematode inhibitor, fungicide and plant growth promoter. The strain has ATCC accession number PTA-3701. The strain is isolated by treating Trichoderma harzianum isolated from experimental fields of Central Institute of Medicinal and Aromatic Plants (CIMAP) Field Station with a mutagen such as ethyl methyl sulphonate, and isolating a whitish and fast growing strain of Trichoderma harzianum.

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

A method for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the method including the steps of providing a receptacle, the receptacle configured to allow filtering of the sample and to reversibly contain the sample and a concentration agent; adding the sample to the receptacle, wherein a microorganism-bound composition will be formed in the receptacle, the microorganism-bound composition including concentration agent-bound microorganisms and sample matrix; and filtering the microorganism-bound composition through a filter to collect the concentration agent-bound microorganisms on the filter, wherein the filter has an average pore size that is greater than the average size of the microorganisms. Kits and systems are also disclosed herein.

Current approaches for treating cancer are limited, in part, by the inability of drugs to affect the poorly vascularized regions of tumors. We have found that spores of anaerobic bacteria in combination with agents which interact with microtubules can cause the destruction of both the vascular and avascular compartments of tumors. Two classes of microtubule inhibitors were found to exert markedly different effects. Some agents that inhibited microtubule synthesis, such as vinorelbine, caused rapid, massive hemorrhagic necrosis when used in combination with spores. In contrast, agents that stabilized microtubules, such as the taxane docetaxel, resulted in slow tumor regressions that killed most neoplastic cells. Remaining cells in the poorly perfused regions of tumors could be eradicated by sponzlated bacteria. Mechanistic studies showed that the microtubule destabilizers, but not the microtubule stabilizers, radically reduced blood flow to tumors, thereby enlarging the hypoxic niche in which spores could germinate. A single intravenous injection of spores plus selected microtubule-interacting agents was able to cause regressions of several tumors in the absence of excessive toxicity.

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

An inoculant composition comprising fungal spores applied to a carrier having a moisture content of not more than about 5% is provided. A method of inoculating a plant to promote growth, enhance resistance to adverse conditions or promote re-growth is also provided comprising applying the inoculant composition to the plant.

The invention discloses paecilomyces lilacinus and an application thereof. The provided paecilomyces lilacinus refers to paecilomyces lilacinus E16 with a register number of CGMCC No.5951 in the common microorganism center of the China microorganism culture preservation management committee. According to the paecilomyces lilacinus E16, in potting experiments, the control effect on second-instar banana root-knot nematodes is 86.37%, the growth rate to banana root weight is 37.76%, the growth rate to banana plant height is 56.47%, the growth rate to overground parts of banana is 38.03%; and in area experiments, the control effect on banana root-knot nematodes is 83.03% and the growth rate to banana fruit is 28.05%.

Embryogenesis from plant microspores is routinely induced with a 16-24 h temperature treatment of 32.5° C. Continuous culture at 25° C. results in pollen development. However, microspore treatment with anti-cytoskeletal agents, or protein synthesis inhibitors, at the non-inductive temperature of 25° C., can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction. Furthermore, when anti-microtubule agents (e.g. colchicine) are used, embryo induction and chromosome doubling occur simultaneously, thus generating doubled haploids, whereas heat induction generates haploids. Thus, the use of microtubule inhibitors will provide a simple one-step process to simultaneously induce embryogenesis and chromosome doubling for the production of fertile plants, thus providing minimal manipulation which will be very advantageous for genetic studies and plant breeding programs. As noted, heat shock induces haploids. A low level of chromosome doubling can be obtained by adding colchicine to microspore cultures during the heat treatment. However, the use of trifluralin with the heat treatment, to generate doubled haploid plants results in an improved recovery of fertile doubled haploid plants than previously shown in the prior art.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The invention discloses trichoderma viride and application thereof. The trichoderma viride is particularly trichoderma viride H06 with CGMCC No. 6229 which has the high prevention and control effect on banana wilt caused by fusarium oxysporum. sp. cubense specialized form No.4 (FOC), wherein the prevention and control effect on banana wilt can be 88%; and the trichoderma viride H06 with CGMCC No.6229 is simple in cultivation conditions, easy to store and industrially produce and excellent in development application prospect.

The invention relates to a method for inducing nematode-trapping fungi to synchronously produce trapping organs, which belongs to the field of applied microbiology. The method comprises the steps of culture of nematodes, preparation of a nematode extract, culture of nematode-trapping fungi and induction of trapping organs. The method can utilize the nematode extract to induce the nematode-trapping fungi to synchronously produce a large number of trapping organs (160 trapping organs / mg mycelium) by culturing the nematodes (Caenorhabditis elegans) and preparing the nematode extract by utilizing ultrasonic waves for crushing and centrifugalizing. The method has the advantages of simple and easy operation and good repeatability, and can obtain a large number of synchronous three-dimensional fungal nets, thereby providing good experimental materials for further research of the molecular mechanism of forming the trapping organs of the nematode-trapping fungi.

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

The process for the production of a chlamydospore rich slurry inoculum begins with a substrate colonized with a desired Basidiomycete fungus capable of producing chlamydospores during vegetative growth. The colonized substrate is treated to increase the chlamydospore production and content in said spawn and thereafter combined with water at rate of at least 1:6 spawn:water to obtain a slurry inoculum. The inoculum may then be agitated to populate a water fraction with chlamydospores or macerated to homogenously distribute the chlamydospores. Soaking of the agitated / macerated inoculum for a time sufficient to further stimulate production of chlamydospores via water shock and obtain a chlamydospore rich slurry

The invention discloses a preparation method of Fuzhuan tea golden flower fungus spore powder, which comprising the steps of: inoculating golden flower fungus to a CZG slant culture medium, culturing for 5days at a temperature of 26-30 DEG C to obtain a seed culture; adding sterile water in the seed culture, washing down golden flower fungus spores, fully oscillating to obtain a uniformly-dispersed spore suspension; inoculating the spore suspension on the culture medium, carrying out constant-temperature culture at 26-30 DEG C, covering with a sterile breathable sealing film before white hypha germinates, removing the sterile breathable sealing film when yellow hypha germinates, continuously culturing for 3-5 days to obtain a yeast seed; then carrying out enlargement to obtain mouldy bran; and crushing the mouldy bran and then sieving to obtain the Fuzhuan tea golden flower fungus spore powder. The artificially cultured Fuzhuan tea golden flower fungus spore powder can be used for extracting effective components of the golden flower fungus and inoculating fermented Fuzhuan tea; and the fermentation period can be shortened and the pollution of infectious microbe is reduced.

The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g / L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.

The present disclosure concerns methods, apparatus, compositions and systems relating to closed bioreactors for algal culture and harvesting. In certain embodiments, the system may comprise bags with various layers, including a thermal barrier layer, that may be used to contain the algal culture and / or to thermally regulate the temperature of the algal culture. The system may comprise various mechanisms for moving fluid within the sytem, such as a roller type mechanism, and may provide temperature regulation by compartmentalization of the fluid to regulate absorption of solar radiation and / or conductive or emissive heat loss and gain. Various mechanisms may be used to harvest and process the algae and / or to convert algal oil into biodiesel and other products.

The biological functionality of living microbial spores is modified using phenotypic engineering to endow the resulting modified spores with novel functionality that extends the usefulness of the spores for a variety of practical applications including, for example, sterility testing, the release of active compounds, and cell-based biosensing systems. A preferred embodiment entails engineering Bacillus spores to acquire synthetic new functions that enable the modified spores to sense and rapidly transduce specific germination signals in their surroundings. The newly acquired functions allow the spores to perform, for example, as self-reporters of cellular viability, self-indicating components of cell-based biosensors, and in other analytical systems.

The present invention refers to a process designed to increase the production of plant growth-promoting microorganisms of the Bacillus genus, using a culture medium poor in nutrients and with specific environmental conditions, allowing to obtain a greater amount of biomass and / or spores, which can be used to prepare solid or liquid compositions to be applied to plants, aiming to promote their growth and / or counteract the effect of phytopathogenic agents.

The invention discloses a method for preparing the spores of bacillus stearothermophilus. The method comprises the steps of culture activation, thallus multiplication, thallus screening and spore induction, wherein 1-5 pieces of sterile filter paper cover the surface of a culture medium during the thallus screening, and 5-10 parts of PEG6000 by weight is added in the culture medium during the spore induction. According to the method disclosed by the invention, the spores can be massively prepared in a short time by improving the formula of the culture medium and the culture conditions; moreover, the culture medium is prepared from cheap and easily available ingredients under simple process conditions within a short production period; the time consumption of one batch of the whole preparation process is within 90 hours; the scheme provides a simple culture method and an industrialized fermentation cultivation route, and the spores produced by induction are high in purity and has a content of 1*10<9> / mL and a conversion rate of greater than 98%.

The invention relates to preparation methods and applications of beauveria bassiana(Bals.-Criv.)Vuill and a bactericide thereof. Bassiana(Bals.-Criv.)Vuill especially has the effect of controlling locust pests. The accession number of bassiana(Bals.-Criv.)Vuill TRCC16003 provided by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.7998. The strain has strong spore producing capacity, has strong insecticidal activity toward three-year-old nymphs of locusts and has LT50 (lethal time of 50%) of 4.13 days. The population quantity of the locusts can be effectively controlled by utilizing the strain. Besides, bassiana(Bals.-Criv.)Vuill has good control effects on other pests such as pine moths, ostrinia nubilalis and grubs and is worthy of popularization and application.

The invention discloses a production method for high-density pure arbuscular mycorrhizal fungal spore, which belongs to the technical field of microorganism culturing, and comprises the following technical steps of: 1) culturing carrot root tissues converted from agrobacterium rhizogenes plasmid DNA; 2) culturing the carrot root tissues converted from agrobacterium rhizogenes plasmid DNA and glomus intraradices together in an improved synthetic culture medium; 3) taking a culture containing the root, hypha and spore infected by the arbuscular mycorrhizal fungus and transplanting the culture to a special culture box for culturing; 4) collecting the spore and mycelium; and 5) storing the arbuscular mycorrhizal fungal spore. Compared with the prior art, the production method for high-density pure arbuscular mycorrhizal fungal spore has the advantages that: 1, the culture medium is an asepsis environment with low cost, which not only can meet the growing of a host plant, but also is suitable for the growing of AM epiphyte; 2, the nutrition requirements for the growing of the host plant are low and the host plant can grow and be cultured on the synthetic culture medium; 3, the spore cultured by the method is a non-pollution pure culture; and 4, the produced spore is stored in liquids and the activity can be maintained by above 90 percent.

The invention provides a method for rapidly identifying resistance to verticillium wilt of eggplant. The method comprises the following steps of: accelerating germination and sowing of eggplant seeds, seedling culturing, preparation of a verticillium wilt spore suspension, treatment of pathogenic bacteria infection and incidence investigation. According to the invention, eggplant seedlings are infected with verticillium wilt in a manner of soaking and infecting seedling roots by a bacteria solution, and the eggplant seedlings are infected after being uniformly cultured in a substrate until achieving a state of one seedling with two leaves, so that the growth vigor and size of the eggplant seedlings are easy to control, and the consistency and reliability of an identification result are ensured. By adopting the appropriate verticillium wilt substrate and adjusting the concentration of the verticillium wilt suspension, the method has the advantages of good infection effect, high survival rate of the infected seedlings, uniform inoculation and rapid incidence. According to the invention, only different plants with consistent growth vigor need to be soaked in the bacteria solution after root cutting, so that the operation is simple and convenient, the seedling culture time is short, and the method is particularly suitable for identification of resistance to verticillium wilt of eggplant breeding materials in batches.

An equipment for culturing microalgae is composed of culturing tank, water supply unit, culture liquid supply unit, air supply unit, CO2 provider, algae liquid collector and artificial light source. Said water supply unit for providing disinfected water consists of water supply pump, water outlet pump, heat exchanger set, water heater, temp-regulating water accumulator, and water source with pump.