471results about "Spore processes" patented technology

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention discloses talaromyces purpurogenus, a preparation method and application in prevention and treatment of corn stalk rot, and provides a novel strain, namely talaromyces purpurogenus Jnf41, greenhouse control effect experiments show that the talaromyces purpurogenus Jnf41 can inhibit the scab area and the scab length of the corn stalk rot in greenhouse control effect determination, and the talaromyces purpurogenus Jnf41 can be used for preventing and treating the corn stalk rot. Infection of the corn stalk rot can be effectively inhibited, pathogenic bacteria are not prone to generating drug resistance, green prevention and control are implemented in the prevention and control process, and the biological preparation for resisting the corn stalk rot is prepared and has important significance on green prevention and control of the corn stalk rot.

The invention discloses talaromyces sp. DFHJ2 and application of a fermentation product of the talaromyces sp. DFHJ2 in strawberry growth promotion and pathogenic bacterium inhibition, and relates to the technical field of microbiology. The talarospora scabra is preserved in the China General Microbiological Culture Collection Center on February 17, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41813. The talarospora scabra is named as talarospora scabra. According to the invention, a talaromyces scabra strain is separated from the root of 'Zhongjiang Junjun' strawberry, and the talaromyces scabra strain has a good antagonistic effect on strawberry anthracnose and other types of strawberry pathogenic bacteria which are seriously harmful in the current production, and can be used for efficiently preventing and treating strawberry anthracnose and other strawberry diseases. In addition, the strain is moderate in growth speed, large in sporulation quantity, easy to ferment and high in adaptability to the pH value of the environment, has a certain growth promoting effect on strawberry seedlings, and has the huge potential of being developed into an efficient biocontrol microbial agent.

The invention discloses a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma preparation, and belongs to the technical field of biocontrol microbial agents. The invention provides a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma virens strain KN-T01 and aims to solve the technical problem that a biocontrol microbial agent in the prior art is poor in microbial function effect in a low-temperature environment, the preservation number of the trichoderma virens strain KN-T01 is CCTCC M 20242443, and the trichoderma preparation contains the trichoderma virens strain KN-T01. The trichoderma virens strain KN-T01 and the trichoderma preparation provided by the invention have relatively good low temperature resistance, have relatively good antagonism and hyperparasitism effects on pathogenic bacteria of pepper gray mold and watermelon fusarium wilt, have a good decomposition effect on straws under a low-temperature condition, and have a relatively good promotion effect on growth of tomatoes and corns under a low-temperature condition, so that the trichoderma virens strain KN-T01 and the trichoderma preparation have relatively good application prospects. The trichoderma virens strain KN-T01 and the trichoderma preparation are non-toxic and harmless, and can be applied to agricultural green prevention and control.

The invention discloses a microbial agent for preventing and treating peanut root rot and a preparation method thereof, and belongs to the technical field of microbial materials. A strain of trichoderma harzianum with an efficient inhibiting effect on pathogenic bacteria of peanut root rot is obtained through screening, firstly, the strain of trichoderma harzianum has an antagonistic effect on the pathogenic bacteria of peanut, secondly, the trichoderma harzianum is prepared into a spore suspension, and the spore suspension is subjected to solid fermentation with rice husks and wheat bran. Metabolite produced by the trichoderma harzianum in the solid-state fermentation process in the fermentation process can promote the growth and development of plant root systems and improve the biomass and yield of plants. On one hand, the microbial agent disclosed by the invention effectively prevents and treats peanut root rot, has a good inhibition effect on pathogenic bacteria of the peanut root rot, and improves the quality and disease resistance of peanuts; on the other hand, the ecological activity of soil is improved to a certain extent, and application and popularization are worthy.

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for producing dark isolated endophytic fungus cladosporium chlamydospore through liquid fermentation. The culture medium and culture conditions suitable for growth of the Cladosporium kwangsiensis HX2 are found out through experiments such as single factor and response surface optimization, the yield of the chlamydospore produced by the Cladosporium kwangsiensis is greatly improved through optimization, the production cost of the chlamydospore is effectively reduced, and the shelf life of the fungicide is prolonged; through measurement and calculation, compared with a traditional PDB culture medium, the yield of chlamydospore is increased by 139.8%, the cost is reduced by 47%, and the shelf life is prolonged by about 8 months. The culture medium is simple in preparation, stable in fermentation process and suitable for large-scale production, the prepared chlamydospore preparation is high in stress resistance and long in shelf life, and a new effective thought is provided for industrialization of bottle cladosporium strains in the later period.

The invention discloses a metarhizium aureoviride strain YEC and application of the metarhizium aureoviride strain YEC in prevention and treatment of teak camel moths. The Metarhizium anisoviride YEC provided by the invention is separated from soil in Guangdong province, can infect mangrove forest pests, namely teak camel moths, and can have relatively high fatality rate and stiff insect rate on the teak camel moths within a short time. The metarhizium aureoviride strain YEC is a biological control strain with potential development and application value in control of mangrove forest pests.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.

The invention discloses Mucor irregularia and application thereof, the strain number of the Mucor irregularia is JSAFC 3408, the Mucor irregularia is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC No.41982. The Mucor irregularia has the advantages that the Mucor irregularia has the advantages that the Mucor irregularia can be used for preparing the Mucor irregularia; according to the application, the soil around the pepper is separated and purified, so that the Mucor irregularia is obtained. The strain can inhibit the growth of the huanghuai glossospora, promote the development of the pepper and regulate the growth of plants, and has great significance for the development of a biocontrol fungicide for preventing and treating the pepper root rot in the future.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention relates to the technical field of biological control, and discloses a hyphantria cunea biocontrol bacterium and application thereof, the biocontrol bacterium is classified and named as Entomortierella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella The hyphantria cunea biocontrol bacterium strain provided by the invention can obviously inhibit the survival activity of hyphantria cunea, the death rate of hyphantria cunea larvae can be increased to 96.7% after 7 days of treatment, and the hyphantria cunea larvae can die in the pupal stage and cannot be subjected to eclosion, so that the hyphantria cunea biocontrol bacterium strain can be used for preventing and treating hyphantria cunea, is typical biological prevention and treatment, can avoid the problems of drug resistance, environmental pollution and the like caused by chemical prevention and treatment, and has broad application prospects. Good application prospects are realized.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The invention discloses Lunasporgiospora chienii and application thereof, the strain number of the Lunasporgiospora chienii is JSAFC 2261, the Lunasporgiospora chienii is preserved in China General Microbiological Culture Collection Center on September 23, 2024, and the preservation number of the Lunasporgiospora chienii is CGMCC No.41524. The invention further discloses a preparation method of the Lunasporgiospora chienii. The ensiformospora strain provided by the invention is strong in pathogenicity to fall webworms and larvae of the larvae of the Artemisia cordata, is environment-friendly and pollution-free, is not easy to generate drug resistance, can be used as a living body biopesticide, and has very strong application potential in biological prevention and control of the fall webworms and / or the larvae of the Artemisia cordata.

The invention belongs to the technical field of building materials, and particularly relates to a preparation method of alkali-treated polypropylene fiber and recycled aggregate loaded bacillus coriolis synergistic microbial concrete. According to the method, a polypropylene fiber reinforced concrete matrix is subjected to alkali treatment, calcium carbonate is generated by combining the mineralization effect of recycled aggregate loaded microorganisms, and efficient crack repair and mechanical property improvement are achieved. The method specifically comprises the steps of microbiological treatment and loading, fiber surface modification and optimization, self-healing concrete preparation and the like. The recycled aggregate is used for fixing microorganisms and providing a mineralization environment, the interface bonding capacity is enhanced through alkali treatment fibers, and the calcium carbonate deposition efficiency is remarkably improved. The prepared self-healing concrete has excellent mechanical strength and compactness, and the repairing performance is also obviously improved. The method is simple in process and controllable in cost, the utilization of the recycled aggregate conforms to the concept of green building materials, and the method can be widely applied to preparation of microbial concrete and has remarkable environmental protection value and application prospects.

The invention discloses trichoderma asperellum TR35 and application thereof in inhibition of mulberry bacterial wilt and prevention and treatment of mulberry bacterial wilt, the trichoderma asperellum TR35 is identified as Trichoderma asperellum, the trichoderma asperellum TR35 is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.64886, and the preservation time is July 18, 2024. The trichoderma asperellum TR35 obtained through separation and screening has good inhibition and antagonism effects on mulberry ralstonia solanacearum, and can be used for preventing and treating mulberry bacterial wilt. The inhibition rate of the trichoderma asperellum TR35 to the mulberry ralstonia solanacearum reaches up to 98.25%, and the active fermentation liquor of the trichoderma asperellum TR35 not only can significantly inhibit the growth of the ralstonia solanacearum and reduce the forming ability of a biological membrane, but also can destroy the integrity and continuity of the biological membrane of the ralstonia solanacearum. In addition, the bacterial zone of the ralstonia solanacearum can be reduced, the athletic ability is reduced, and the expression of virulence-related genes in the ralstonia solanacearum is remarkably inhibited.

The invention discloses radix ophiopogonis endophytic fungi and application thereof, and belongs to the technical field of plant disease prevention and control. The endophytic fungi are separated from liliaceae ophiopogon plant ophiopogon japonicus living bodies by adopting an endophytic fungi separation and purification technology, belong to xylaria, xylaria and carbendazim fungi, are classified and named as Nemania diffusa OJ51, and have a strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41084. The endophytic fungus can inhibit mycelial growth of pathogenic bacteria, namely Fusarium oxysporum and Fusarium solani, of radix ophiopogonis root rot, the relative inhibition rates are 63.51 + / -1.22% and 66.27 + / -1.03% respectively, meanwhile, the disease condition of radix ophiopogonis root rot is effectively relieved, and the disease index is reduced by 76.70%. The endophytic fungus has an obvious effect of preventing and treating radix ophiopogonis root rot, and a wide application prospect is brought to the field of biological prevention and treatment of radix ophiopogonis fungus diseases.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

A method for producing fungal spores, wherein the fungal spores are grown in a first liquid medium and then in a second liquid medium, the first liquid medium having a lower carbon content than the second liquid medium.

The invention provides a new anti-tumor ganoderma lucidum variety, a related product as well as a preparation method and application of the new anti-tumor ganoderma lucidum variety, and belongs to the field of new ganoderma lucidum varieties. The new variety of ganoderma lucidum provided by the invention is classified and named as Ganoderma sichuanense Xianzhi No.13, and is preserved in the China Center for Type Culture Collection (CCTCC) on October 25, 2024, and the preservation number is CCTCC NO: M 20242347. Compared with the existing ganoderma lucidum variety, the new ganoderma lucidum variety provided by the invention has higher yield and higher content of effective components and anti-cancer active components, and the extract of the ganoderma lucidum has good anti-tumor activity.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The invention discloses a Lentinus edodes strain SICAU-F2-1283 and application thereof, the strain is preserved in China Center for Type Culture Collection on April 14, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41891. The Lentinus edodes strain SICAU-F2-1283 has been preserved in China Center for Type Culture Collection. The lentinus edodes strain provided by the invention has the advantages of more fruiting quantity in a wheat straw culture medium, large single mushroom mass, high biological efficiency and excellent agronomic traits. The new mushroom variety provided by the invention solves the problems of low yield and poor quality of mushrooms in wheat straw cultivation, provides choices of more varieties of different types for the edible mushroom industry, and has a remarkable application prospect in the field of mushroom planting.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses a method for promoting growth or resisting diseases of oilseed rape. The research finds that any one or a combination of more of bacillus subtilis B.subtilis 168, trichoderma harzianum T.harzianum 25852 and trichoderma harzianum T.harzianum 52623 can effectively promote the growth of rape seedlings, and the growth mainly shows that the plant stem diameter, the plant height and the increase of the biomass of the overground part and the underground part. Wherein the trichoderma harzianum T.harzianum 25852, and the combination of the bacillus subtilis B.subtillis 168 and the trichoderma harzianum T.harzianum 25852 also have a relatively good sclerotinia rot prevention and treatment effect. The microorganism provided by the invention has the characteristics of high efficiency, no pollution, difficult generation of drug resistance and the like in the aspect of oilseed rape production, has great application potential, and has important significance for promoting the improvement of oil productivity and green low-carbon efficient production in China.

The invention discloses beauveria bassiana ZJB24327 and an application of the beauveria bassiana ZJB24327. The beauveria bassiana ZJB24327 is high in sporulation quantity and good in ultraviolet tolerance, and has a very strong infection insecticidal effect on plutella xylostella, the sporulation quantity can reach 2.508 * 10 < 8 > / cm < 2 > in 10 days under an optimal condition, and the germination rate still reaches 8.14 + / -1.80% after 25 minutes of ultraviolet treatment. The method is suitable for being applied to high-altitude areas, and lays a foundation for large-scale production of beauveria bassiana.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention relates to the technical field of microorganisms, in particular to a cotton bollworm metarhizium reesei MRDL2024 strain and application thereof.The classification name of the metarhizium reesei MRDL2024 is metarhizium reesei MRDL2024, and the preservation number of the metarhizium reesei MRDL2024 is CGMCC No.41555. The metarhizium reesei MRDL2024 is easy to culture, rapid in growth, large in sporulation quantity, high in spore germination rate and easy to culture and breed in a large amount indoors, and the metarhizium reesei MRDL2024 can be widely applied to the field of agricultural production. The fungus is a biocontrol fungus which can be used for preventing and controlling the pest cotton bollworm which harms crops such as corn and tobacco, has the characteristics of strong pathogenicity to the larva of the cotton bollworm, safety to the environment and difficulty in causing the pest to generate drug resistance, and can be used for biologically preventing and controlling the cotton bollworm.

The invention relates to the technical field of biologic stimulant preparation, and discloses a biologic stimulant mixing preparation two-stage control method, which comprises: adopting dissolved oxygen gradient and carbon source pulse cooperative control, linearly reducing the concentration of dissolved oxygen in a fermentation tank from an initial value at a specific rate, and injecting a carbon source at regular time; when the flora density and the quorum sensing signal molecule concentration reach specific threshold values, triggering stage switching; starting pressure-metabolism coupling control, increasing the pressure of the fermentation tank at a specific rate, and adding coenzyme Q10; the quorum sensing inhibitory peptide containing a specific core structure is synchronously released in the boosting process; after the pressure is stable, a real-time mapping relation between a microbial metabolism state and physical parameters is constructed in a thallus proliferation stage through a coupling mechanism of dissolved oxygen gradient control and quorum sensing signal monitoring, and the industrial problem that a traditional static threshold value cannot respond to pressure fluctuation of a fermentation tank to cause signal attenuation is solved.

The invention provides a streptomyces graminearus strain, a streptomyces graminearus oil suspension, as well as its preparation method and application thereof. The streptomyces graminearus oil suspension contains streptomyces graminearus conidiospore powder, emulsifier, stabilizer, wetting dispersant, antioxidant, thickener, ultraviolet protectant, preservative and carrier oil. The use of carrier oil as the dispersion medium offers environmental friendliness, excellent permeability, adhesion properties, and resistance to rain washing. Additionally, the carrier oil enhances the spore germination of streptomyces graminearus. Furthermore, it has the advantages of low application cost, slow spore settling speed, easy to shake well when using, stable efficacy, long storage time, suitability for various spraying techniques and a simple preparation method. The spore survival time of the streptomyces graminearus oil suspension is prelonged, with a spore germination rate is as high as 85% after 18 months of storage. It demonstrates effective control against yam, peanut and forest nematode.