365results about "Spore processes" patented technology

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention discloses talaromyces purpurogenus, a preparation method and application in prevention and treatment of corn stalk rot, and provides a novel strain, namely talaromyces purpurogenus Jnf41, greenhouse control effect experiments show that the talaromyces purpurogenus Jnf41 can inhibit the scab area and the scab length of the corn stalk rot in greenhouse control effect determination, and the talaromyces purpurogenus Jnf41 can be used for preventing and treating the corn stalk rot. Infection of the corn stalk rot can be effectively inhibited, pathogenic bacteria are not prone to generating drug resistance, green prevention and control are implemented in the prevention and control process, and the biological preparation for resisting the corn stalk rot is prepared and has important significance on green prevention and control of the corn stalk rot.

The invention discloses talaromyces sp. DFHJ2 and application of a fermentation product of the talaromyces sp. DFHJ2 in strawberry growth promotion and pathogenic bacterium inhibition, and relates to the technical field of microbiology. The talarospora scabra is preserved in the China General Microbiological Culture Collection Center on February 17, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41813. The talarospora scabra is named as talarospora scabra. According to the invention, a talaromyces scabra strain is separated from the root of 'Zhongjiang Junjun' strawberry, and the talaromyces scabra strain has a good antagonistic effect on strawberry anthracnose and other types of strawberry pathogenic bacteria which are seriously harmful in the current production, and can be used for efficiently preventing and treating strawberry anthracnose and other strawberry diseases. In addition, the strain is moderate in growth speed, large in sporulation quantity, easy to ferment and high in adaptability to the pH value of the environment, has a certain growth promoting effect on strawberry seedlings, and has the huge potential of being developed into an efficient biocontrol microbial agent.

The invention discloses a microbial agent for preventing and treating peanut root rot and a preparation method thereof, and belongs to the technical field of microbial materials. A strain of trichoderma harzianum with an efficient inhibiting effect on pathogenic bacteria of peanut root rot is obtained through screening, firstly, the strain of trichoderma harzianum has an antagonistic effect on the pathogenic bacteria of peanut, secondly, the trichoderma harzianum is prepared into a spore suspension, and the spore suspension is subjected to solid fermentation with rice husks and wheat bran. Metabolite produced by the trichoderma harzianum in the solid-state fermentation process in the fermentation process can promote the growth and development of plant root systems and improve the biomass and yield of plants. On one hand, the microbial agent disclosed by the invention effectively prevents and treats peanut root rot, has a good inhibition effect on pathogenic bacteria of the peanut root rot, and improves the quality and disease resistance of peanuts; on the other hand, the ecological activity of soil is improved to a certain extent, and application and popularization are worthy.

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for producing dark isolated endophytic fungus cladosporium chlamydospore through liquid fermentation. The culture medium and culture conditions suitable for growth of the Cladosporium kwangsiensis HX2 are found out through experiments such as single factor and response surface optimization, the yield of the chlamydospore produced by the Cladosporium kwangsiensis is greatly improved through optimization, the production cost of the chlamydospore is effectively reduced, and the shelf life of the fungicide is prolonged; through measurement and calculation, compared with a traditional PDB culture medium, the yield of chlamydospore is increased by 139.8%, the cost is reduced by 47%, and the shelf life is prolonged by about 8 months. The culture medium is simple in preparation, stable in fermentation process and suitable for large-scale production, the prepared chlamydospore preparation is high in stress resistance and long in shelf life, and a new effective thought is provided for industrialization of bottle cladosporium strains in the later period.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.

The invention discloses Mucor irregularia and application thereof, the strain number of the Mucor irregularia is JSAFC 3408, the Mucor irregularia is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC No.41982. The Mucor irregularia has the advantages that the Mucor irregularia has the advantages that the Mucor irregularia can be used for preparing the Mucor irregularia; according to the application, the soil around the pepper is separated and purified, so that the Mucor irregularia is obtained. The strain can inhibit the growth of the huanghuai glossospora, promote the development of the pepper and regulate the growth of plants, and has great significance for the development of a biocontrol fungicide for preventing and treating the pepper root rot in the future.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The invention belongs to the technical field of building materials, and particularly relates to a preparation method of alkali-treated polypropylene fiber and recycled aggregate loaded bacillus coriolis synergistic microbial concrete. According to the method, a polypropylene fiber reinforced concrete matrix is subjected to alkali treatment, calcium carbonate is generated by combining the mineralization effect of recycled aggregate loaded microorganisms, and efficient crack repair and mechanical property improvement are achieved. The method specifically comprises the steps of microbiological treatment and loading, fiber surface modification and optimization, self-healing concrete preparation and the like. The recycled aggregate is used for fixing microorganisms and providing a mineralization environment, the interface bonding capacity is enhanced through alkali treatment fibers, and the calcium carbonate deposition efficiency is remarkably improved. The prepared self-healing concrete has excellent mechanical strength and compactness, and the repairing performance is also obviously improved. The method is simple in process and controllable in cost, the utilization of the recycled aggregate conforms to the concept of green building materials, and the method can be widely applied to preparation of microbial concrete and has remarkable environmental protection value and application prospects.

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

A method for producing fungal spores, wherein the fungal spores are grown in a first liquid medium and then in a second liquid medium, the first liquid medium having a lower carbon content than the second liquid medium.

The invention provides a new anti-tumor ganoderma lucidum variety, a related product as well as a preparation method and application of the new anti-tumor ganoderma lucidum variety, and belongs to the field of new ganoderma lucidum varieties. The new variety of ganoderma lucidum provided by the invention is classified and named as Ganoderma sichuanense Xianzhi No.13, and is preserved in the China Center for Type Culture Collection (CCTCC) on October 25, 2024, and the preservation number is CCTCC NO: M 20242347. Compared with the existing ganoderma lucidum variety, the new ganoderma lucidum variety provided by the invention has higher yield and higher content of effective components and anti-cancer active components, and the extract of the ganoderma lucidum has good anti-tumor activity.

The invention discloses a Lentinus edodes strain SICAU-F2-1283 and application thereof, the strain is preserved in China Center for Type Culture Collection on April 14, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41891. The Lentinus edodes strain SICAU-F2-1283 has been preserved in China Center for Type Culture Collection. The lentinus edodes strain provided by the invention has the advantages of more fruiting quantity in a wheat straw culture medium, large single mushroom mass, high biological efficiency and excellent agronomic traits. The new mushroom variety provided by the invention solves the problems of low yield and poor quality of mushrooms in wheat straw cultivation, provides choices of more varieties of different types for the edible mushroom industry, and has a remarkable application prospect in the field of mushroom planting.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention relates to the technical field of biologic stimulant preparation, and discloses a biologic stimulant mixing preparation two-stage control method, which comprises: adopting dissolved oxygen gradient and carbon source pulse cooperative control, linearly reducing the concentration of dissolved oxygen in a fermentation tank from an initial value at a specific rate, and injecting a carbon source at regular time; when the flora density and the quorum sensing signal molecule concentration reach specific threshold values, triggering stage switching; starting pressure-metabolism coupling control, increasing the pressure of the fermentation tank at a specific rate, and adding coenzyme Q10; the quorum sensing inhibitory peptide containing a specific core structure is synchronously released in the boosting process; after the pressure is stable, a real-time mapping relation between a microbial metabolism state and physical parameters is constructed in a thallus proliferation stage through a coupling mechanism of dissolved oxygen gradient control and quorum sensing signal monitoring, and the industrial problem that a traditional static threshold value cannot respond to pressure fluctuation of a fermentation tank to cause signal attenuation is solved.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

The invention provides aspergillus scherweri for high-yield cellulase and application of the aspergillus scherweri in fermentation of Chinese herbal medicines. The Aspergillus scherweri J-10 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Aspergillus scherweri J-10 is CGMCC No. 41630. The Aspergillus scherweri J-10 disclosed by the invention has the advantages that the Aspergillus scherweri J-10 can be used for preparing the Aspergillus When the aspergillus scherweri provided by the invention is used for fermenting Chinese herbal medicines represented by lucid ganoderma, the release and utilization rate of active ingredients of the Chinese herbal medicines can be remarkably improved. An in-vitro anti-tumor activity result shows that the alcohol extract of the Chinese herbal medicine (represented by ganoderma lucidum sporocarp) fermented by the J-10 strain has a stronger in-vitro inhibition effect on various tumor cells, and a new method and way are provided for deep processing and comprehensive utilization of the Chinese herbal medicine.

The invention relates to a preparation method of a multifunctional nano growth-promoting bacterium for overcoming successive cropping obstacles, which comprises the following steps: converting bacillus subtilis into bacillus subtilis spores under the condition of heating or inoculating bacillus subtilis into a sporulation culture medium containing manganese sulfate, preparing iron nanoparticles by using a chemical reducing agent and a coprecipitation method under the protection of inert gas, and carrying out vacuum drying on the iron nanoparticles to obtain the multifunctional nano growth-promoting bacterium for overcoming successive cropping obstacles. The preparation method is simple, the cost is low, the production process is green and environment-friendly and suitable for large-scale production, the prepared nano growth-promoting bacteria can integrate the biological functions of the iron nanoparticles and the bacillus subtilis, the soil fertility is improved, trace elements are supplemented, and meanwhile the biological functions of the iron nanoparticles and the bacillus subtilis are improved. The fertilizer promotes proliferation of beneficial bacteria, inhibits proliferation of harmful bacteria, improves the utilization rate of nutrients, degrades harmful substances, improves stress resistance of crops, realizes dual promotion of soil improvement and crop growth, and has remarkable social and economic benefits.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The application discloses a Penicillium oxalicum strain, a biocontrol agent and application. A fungus is isolated and screened from soil, and the fungus is identified as Penicillium oxalicum by combining morphological characteristics and molecular identification, and is named as Penicillium oxalicum PO14 with a preservation number of CGMCC No.22461. The Penicillium oxalicum PO14 has a strong inhibitory effect on Fusarium fujikuroi spore germination and mycelium growth, and can be used for prevention and treatment of rice bakanae disease. The Penicillium oxalicum PO14 strain can be used for development of a biocontrol agent or a biological pesticide, and has a good application prospect in biological prevention of rice bakanae disease.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The invention provides coremia lecanii for preventing and treating Coptotermes formosanus and application thereof, in particular to a suspension preparation thereof. The strain number of the lecanicillium lecanii is NBCC-L5, and the lecanicillium lecanii is preserved in the China Center for Type Culture Collection and has a remarkable effect of preventing and treating Coptotermes formosanus. The strain rapidly propagates in termite bodies through spores of the strain, so that the termite bodies die, and the strain has the advantages of being green, environment-friendly and non-toxic, does not easily generate drug resistance, and can stably play a prevention and treatment effect for a long time. The coremia lecanii suspension preparation is suitable for termite control, the spore concentration can be adjusted to 1 * 10 < 3 >-1 * 10 < 7 > spore / mL, and the coremia lecanii suspension preparation can be dropwise added to the chest and back plate of termites or other exposed areas for precise treatment in practical application. Compared with an existing chemical prevention and control method, the biological prevention and control scheme not only improves the stability of the prevention and control effect, but also can effectively avoid environmental pollution, and an economic, environment-friendly and sustainable termite prevention and control solution is provided.

A method for producing fungal spores using solid-state fermentation (SSF), comprising: combining fungal spores and a solid substrate in a solid-state fermentor to produce a fungal culture; subjecting the fungal culture in the solid-state fermentor to solid-state fermentation conditions, which include conditions for growth of the fungal cells for the targeted production of additional fungal spores by the fungal cells; and harvesting the additional fungal spores.

The invention provides red trametes sanguineus with high yield of active spores and a culture method of the red trametes sanguineus, belongs to the technical field of microorganisms, and particularly provides red trametes sanguineus HSJ1029 which is preserved in China General Microbiological Culture Collection Center on September 14, 2023, is located in No.1 yard, Beichen West Road, Chaoyang District, Beijing, and has a preservation number of CGMCC No.40866. The invention further provides a culture method of the red trametes sanguineus HSJ1029. The invention also provides a culture method of the high-yield red trametes spore. The invention provides a strain of Rhodobryum sanguineum HSJ1029, the strain is high in sporulation ability, and the inventor discovers that Rhodobryum sanguineum HSJ1029 spores contain the cinnabine for the first time.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.