194results about How to "Reduce browning rate" patented technology

The invention discloses a method for tissue culture method of huperizia serrata. The method comprises the following steps of: selecting an explant; killing surface bacteria; performing inoculation and cultivation; killing endophyte; performing propagation cultivation; and performing rooting cultivation. The huperizia serrata is a precious medicinal pteridophyte which has a tough requirement on a habitat, grows slowly under a natural condition and has a long production period. In a conventional method, propagation coefficient and biomass are low and requirements on medicament production cannot be met. Due to the adoption of the method, the differentiation rate of a huperizia serrata test tube plantlet is up to 90 percent, monthly multiplication multiple is up to 2.1 to 3.5, rooting rate is up to 100 percent, a root system grows quickly, root number is up to 1.6 to 3.4 per plant, a regeneration seedling grows healthily and the root system is developed. The method has the advantages of effectively restraining the pollution and browning of a huperizia serrata explant, promoting the survival and growth of the explant and providing important technical support for the realization of manual mass planting of the huperizia serrata along with simpleness, convenience, practicability, high efficiency and low cost.

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The invention discloses a sustained-released micro-capsule freshly-cut fruit and vegetable preservative and a production method and application thereof, and belongs to the technical field of food fresh keeping. According to the sustained-released micro-capsule preservative, chitosan and sodium alginate are taken as raw materials, a chitosan wall material solution and a sodium alginate sodium wallmaterial solution are prepared to be mixed with polysorbate 80, one of cinnamon essential oil, thyme essential oil and oregano essential oil is added, or the three above essential oils are added simultaneously according to a proportion to obtain a sustained-released micro-capsule preservative. The preservative is use for the fresh keeping of freshly cut fruits and vegetables. The invention provides an essential oil compound, a better synergistic effect can be generated, the invasion and growing proliferating of microorganisms on the freshly cut fruits and vegetables can be effectively inhibited, the enzymatic browning reaction of the fruits and the vegetables can be effectively inhibited, the browning speed of the fruits and the vegetables are slowed down, and the color chrominance of thefruits and the vegetables are well maintained.

The invention relates to a plant tissue culture method, in particular to a tissue culture method of toxicodendron vernicifluum, and particularly relates to a high-efficiency and rapid micropropagation method for toxicodendron vernicifluum. The high-efficiency and rapid micropropagation method for toxicodendron vernicifluum comprises the following steps: (1) selecting and disinfecting an explant; (2) starting culture; (3) conducting enrichment culture; (4) conducting rooting culture; and (5) hardening seedlings and transplanting. The toxicodendron vernicifluum has the advantages of being stable in inheritable character, simple in culture process, easy to root, short in culture micropropagation period, and easy to survive after being transplanted. By adopting a method for conducting tissue culture on excellent single plants of toxicodendron vernicifluum, the propagation coefficient can achieve four to five times, the annual propagation amount of every ten effective toxicodendron vernicifluum axillary buds can still achieve more than 1.75 million calculated according to the propagation coefficient of 4.5 and the propagation period of 45 days despite pollution loss, the rapid propagation problem of the toxicodendron vernicifluum can be effectively solved, the excellent clone materials can be popularized on a large area, and foundation is laid for the industrial seedling.

The invention discloses a culture medium special for preventing brown stain of butterfly orchids, which is prepared by taking a 1 / 2 MS culture medium containing 2.5 mg / L of benzyl amino adenine (BA) and 0.2 mg / L of naphthylacetic acid (NAA) as a basic culture medium and adding vitamin C (VC) with the concentration of 5-15 mg / L and brassinolide (BR) with the concentration of 0.001-0.5 mg / L. The culture medium disclosed by the invention is capable of greatly reducing the brown stain rate in the butterfly orchid tissue culture process.

According to the invention, young leaves or stems of plants of Psammosilene tuniceoides W. C. Wu et C. Y. Wu are taken as an explant to successfully induce callus of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu, and a callus culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu under the conditions of light and dark cultivation is established to induce the differentiation of the callus to produce adventitious roots, thus establishing an adventitious root cultivation system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Moreover, the content of total saponins of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu is determined, and the conditions and parameters of plant cell culture are further optimized, thus establishing a high-yield cell culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Therefore, the plant cells of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu are cultured in a large scale to produce the adventitious roots which substitute for the original plant of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu to be used as medicine.

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.

The invention belongs to the field of plant tissue culture of plant cell engineering techniques, and in particular relates to a tissue culture propagation method for reducing the tissue culture browning rate of traxacum koksaghyz. The tissue culture propagation method comprises the following steps: inoculating, namely, selecting mature seeds of a traxacum koksaghyz strain; performing induced differentiation on calluses; inducing callus formation; proliferating adventitious buds; performing rooting culture; separating multiple shoots into single plants, and transferring to a rooting culture medium for rooting culture; performing acclimatization and transplantation, thereby obtaining regenerated plants of traxacum koksaghyz. By adopting the tissue culture propagation method, a method for effectively reducing the tissue culture browning rate of traxacum koksaghyz and rapidly propagating traxacum koksaghyz is provided, the tissue culture browning rate of traxacum koksaghyz is greatly reduced through technical measures such as adjusting components of culture mediums used in different stages and adding an antioxidant, the propagation coefficient of traxacum koksaghyz is improved, the browning rate can be reduced to be less than 35% when the culture mediums provided by the invention is adopted, and the browning rate can be further reduced to be less than 10% when Na2S2O3 is added.

The invention discloses a rapid propagation method for holcoglossum flavescens. Rapid propagation of holcoglossum flavescens is completed through the steps of seed germination, induction of protocorm, differentiation culture, strong seedling culture, rooting culture, acclimatization and transplant and the like. Compared with a traditional division method, the growth coefficient of holcoglossum flavescens is improved by more than 5 times, the planting percent reaches 95%, the survival rate of transplanting reaches more than 85%, meanwhile, the method has the advantages of being large in increment coefficient, strong in tillering capacity, high in growth speed, strong in plant, low in browning ratio, high in survival rate of transplanting, and low in cost, culture medium is easy to prepare, and the method can be applied to large scale industrial production.

The invention discloses a preparation method of a biological organic fertilizer for improving the fresh juice quality of Fuji apples. The method comprises the following steps: 1, preparing a fish scale enzymatic hydrolysis broth; 2, preparing a fermented rice straw material; 3, preparing a fermented cow and sheep dung material; 4, mixing the fish scale enzymatic hydrolysis broth, the fermented rice straw material and the fermented cow and sheep dung material, inoculating an EM inoculant, and carrying out secondary fermentation; and 5, air-drying, crushing, and carrying out bagging preservation. The biological organic fertilizer obtained through respective fermentation of various organic raw materials, mixing and secondary fermentation is rich in highly active amino acids, proteins, various micro and trace elements and beneficial microbes, can meet the growth demands of Fuji apples, improves the output and quality, improves the soil quality, especially improves the fresh juice quality of the Fuji apples, delays the browning speed of the juice, improves the mouthfeel quality, facilitates application of the Fuji applies in the field of fresh fruit juices, and has wide popularization values.

The invention discloses a preservation method of gerbera germplasm resources. The method comprises the following steps: selecting gerbera flowers during the period from blooming of ray florets to pollination of tubiform florets; keeping flower stalks in the length of 5-6 cm; carrying out induction culture on treated explants; continuously sub-culturing through two subculture mediums with different prescriptions; carrying out rooting culture in a greenhouse; and culturing in a seedling-growing bag and growing for one year in the greenhouse. The gerbera germplasm resources can be preserved for 3-4 years in one preservation cycle and the field survival rate of germplasm resource materials reaches more than 90%. According to the preservation method, the gerbera germplasm resources are preserved through successive subculture of the two different subculture mediums and renovation in the greenhouse, the problems of germchit variation and quality degradation caused by hormone factors, culture environments and the like are reduced, the excellent traits of the gerbera germplasm resources are stably preserved and a new and effective preservation method of the gerbera germplasm resources can be provided.

The invention discloses a method applied to tissue culture and rapid propagation of chimonanthus nitens. The method sequentially comprises the following steps: obtaining aseptic seedlings; taking mature chimonanthus nitens fruits and peeling seeds with complete seed coat; inducing callus and inducing adventitious buds; cutting off cotyledon, cutting the cotyledon into small slices with area of 1 square centimeter by a scalpel, inoculating the small slices in a minimal medium; proliferating and seedling the adventitious buds; cutting off the differentiated buds from the callus, and transferring the differentiated buds into a proliferation culture medium to carry out proliferation culture; rooting rootless seedlings; cutting off strong adventitious buds when the adventitious buds grow to be 3-4cm high, and inoculating the strong adventitious buds in a rooting culture medium; hardening and transplanting seedlings of tissue culture regeneration plants; hardening the seedlings when the seedlings of to-be-rooted tissue culture regeneration plants are 5cm high, the root number is greater than 3 and the root length is greater than 5cm, pounding the culture medium and taking out the plants after three days and burying the roots of the regeneration plants in the culture soil. The chimonanthus nitens cultured by the method is capable of quickly rooting and is low in vitrifying and browning rate and high in survival rate.

The invention discloses a colored cotton ovule in vitro culture method, which comprises the steps of: collecting 0-2 days old young bolls of green cotton or brown cotton of the day, pulling off the corolla, stripping calyxes, and retaining flower stalks; washing the young bolls with distilled water twice, conducting disinfection with 75% alcohol for 5-15min under a sterile condition, then performing soaking with 0.1% mercury chloride for 5-15min, and conducting washing with sterile water 5-8 times, and stripping ovules; inoculating the ovules under a sterile condition into a BT medium containing hormone ZR, GA3 and IAA, inoculating 15-25 ovules of one ovary into each bottle, and carrying out dark culture for 30-40d at 30-32DEG C. Preferably, during inoculation of green cotton ovules, the hormone ratio wais GA31.0micromol / L+IAA5.0micromol / L+ZR2.5micromol / L, and during inoculation of brown cotton ovules, the hormone ratio is GA31.0micromol / L+IAA1.0micromol / L+ZR1.0micromol / L. The method provided by the invention can reduce the contamination rate, improve the survival rate, and promote cotton fiber growth and pigment synthesis.

The present invention relates to a culture media for peony high frequency embryogenic callus differentiation, and a culture method thereof. The culture method comprises steps of material selecting, seed embryo sterilizing and separating, culture media preparing, callus differentiation and embryo bud differentiation. The culture media for the peony callus differentiation is prepared by adding 0.5-2.0 mg / L of NAA, 0.1-0.5 mg / L of TDZ, 30 g / L of sucrose and 6 g / L of agar to a modified 1 / 2MS basic culture media, wherein the pH value is 5.8. The culture media for the peony embryo bud differentiation is prepared by adding 1.0-5.0 mg / L of TDZ, 100 g / L of sucrose and 6 g / L of agar to the modified 1 / 2MS basic culture media, wherein the pH value is 5.8. The culture media and the culture method of the present invention have the following advantages that: the raw materials are easily obtained; the callus rate is high; the browning rate is low; the embryo bud differentiation rate is high; the generesearch and the gene application requirements can be met.

The present invention discloses a chestnut preservation method and belongs to the technical field of chestnut preservations. The preservation method consists of the following steps: (1) fruit selecting: insect pest free, full and harmless chestnuts are selected; (2) preservative treating: the selected chestnuts are soaked with the preservative for 15-35 minutes; (3) clean water soaking: the treated chestnuts in the step (2) are soaked with the clean water at 40 to 50 DEG C for 20-40 minutes; (4) biological treating: the treated chestnuts in the step (3) are soaked with water solution containing endogenetic bacteria for 15-25 minutes, and the soaked chestnuts are air-dried; (5) water content controlling: the treated chestnuts in the step (4) are freeze-dried or cold-air dried to control the moisture content to be 38-44%; and (6) refrigerating: the treated chestnut in the step (5) are put into a cold storage to be preserved at a humidity of 85-95% and a temperature of -4 to 0 DEG C. The endogenetic bacteria are endogenetic bacteria separated from the chestnuts to be preserved and at least have both obvious inhibitions on fusarium solani and colletotrichum gloeosporioides.

The invention discloses a method for preventing daemonorops margaritae callus browning phenomena from occurring. Through preserving young and tender stem sections with terminal buds of fresh daemonorops margaritae in a refrigerated manner, and disinfecting and sterilizing the stem sections by using alcohol and mercuric chloride before the stem sections are inoculated, utilizing middle-later stage combined culture of callus culture and continuously spinning bottles, the daemonorops margaritae browning phenomena are effectively prevented or even eliminated, the browning rate is reduced, the induction rate of the daemonorops margaritae is increased to a certain degree, the growth of plant is promoted and rapid breeding of daemonorops margaritae seedlings is guaranteed, so sa to effectively guarantee the supply of the daemonorops margaritae seedlings and provide support for industrialization development of the daemonorops margaritae.

The invention belongs to the field of plant propagation, and discloses a base material for plant air layering and an application thereof. The base material comprises a solid component and a liquid component, wherein the liquid component contains a plant source anti-browning factor and a plant source rooting-promoting factor, and the solid component and the liquid component are blended according to a volume ratio of (8-9):(2-1) and are mixed uniformly by stirring in use. The components of the base material are derived from natural raw materials, so that the base material can be applied to an organic planting system and the air layering propagation of shrubby or woody plants, and can effectively improve the survival rate of plant air layering.

The invention discloses a tissue culture method for in-vitro regeneration of a grape stem segment explant. The method comprises the following steps: selecting an appropriate grape stem segment explant, sterilizing the grape stem segment explant, inoculating the sterilized grape stem segment explants, building an in-vitro regeneration system for the grape stem segment explant, carrying out grape test-tube plantlet multiplication culture, and performing grape test-tube plantlet rooting culture, thereby achieving regeneration culture of grape tissues. Compared with existing similar studies, the method has the advantages that the adventitious bud has the high regeneration rate, the browning rate is low, the growth coefficient can reach 5.6, and the rooting rate can reach 98.7%. The method is applied to the rapid in-vitro grape reproduction, the reproductive cost is reduced greatly, and the method can be used for provided convenience for correlational research of grape breeding such as grape biotechnological breeding and molecular breeding.

The invention provides a tissue culture method of acer rubrum, comprising the following steps: cutting dormant buds from acer rubrum plants and pretreating, inoculating the pretreated dormant buds to an inducing medium to obtain aseptic shoots; inoculating the obtained aseptic shoots to an inducing medium to obtain crowd shoots; selecting robust crowd shoots and inoculating the robust crowd shoots to a strong seedling medium to obtain robust seedlings; selecting robust seedlings to a rooting medium to obtain regeneration plants; hardening seedling on the regeneration plants in an open hardening seedling room for 3d, washing the medium attached to the root system, transplanting the plants into a culture medium and watering, covering the mouth of a container with a plastic film until new leaves are fully expanded, and removing the covered plastic film so as to obtain the acer rubrum nursery stock. The tissue culture method of acer rubrum has scientific and reasonable steps, is simple and efficient, and has advantages of low cost, high inductivity, high multiplication coefficient and high survival rate.

The invention discloses a method capable of effectively inhibiting browning for the multiplication culture of buckwheat callus, which is characterized in that before the multiplication culture, the buckwheat callus is pre-cultured, and citric acid and L-cysteine are added into a multiplication culture medium. Therefore, the browning rate of the buckwheat callus during the multiplication culture is effectively inhibited, the multiplication times of the buckwheat callus is increased, and a new method is provided for greatly and quickly producing flavonoids (rutin).

The invention discloses a processing method of dry lily pieces. The processing method disclosed by the invention has the advantages that a far-infrared drying box is adopted for drying boiled lily pieces in the processing process, the phenomenon that the lily pieces are browned and blackened in the processing and drying processes is overcome, the processed lily pieces are low in foaming rate, naturally milk-white in color and high in drying efficiency, the production cost is greatly reduced and the original flavor of the lily is guaranteed.

The invention relates to a rapid propagation method for Chinese roses. The method comprises steps as follows: preparation of an explant, disinfection of the explant, induction culture, multiplication culture, rooting culture and transplanting. The tissue culture method for the Chinese roses is optimized, the production procedures are simplified, the growth cycle is shortened, and the survival rate is increased.

The invention belongs to the technical field of agricultural products, and particularly relates to a probiotics fermented type composite fruit and vegetable beverage for moistening and relaxing bowel and a preparation method thereof. The beverage is prepared from, by weight, 40-50 parts of yellow peach puree, 20-30 parts of carrot puree, 10-20 parts of composite berry puree, 5-10 parts of a sweetener substituent solution, and 3.5-7 parts of a sour agent substituent solution. The beverage is produced through a freeze squeezing technology, wherein the fruits and vegetables are subjected to cell disruption after low-temperature freezing, so that juice yield and dissolution of active components are improved. Through compounding of the raw materials, the beverage has a bright color, good stability and fine and smooth mouth feel, is refreshing and has a moderate sour-and-sweet taste, has no bubble and impurities, is free of layering and precipitation, and is color-retaining and glossy, has excellent health care value, is improved in the effect of moistening and relaxing bowel, and is healthy and safe.

The invention discloses a production method of vacuum browning-resistant quick-frozen fruit dices. The production method concretely comprises the following steps: 1, selecting a raw material; 2, immersing: putting the selected raw material in a color protection solution composed of water, D-sodium erythorbate and citric acid, and carrying out color protection; 3, dividing the raw material, and coring the raw material; 4, disinfecting the cored raw material in a D-sodium erythorbate solution; 5, putting the disinfected raw material in a sulfur-free liquid protection solution, and carrying out primary color protection; 6, dicing the raw material; 7, carrying out vacuum pumping, and carrying out secondary color protection; 8, quickly freezing color protected dices; 9, choosing the quick-frozen dices, and bagging the dices; 10, carrying out metal detection; and 11, storing the obtained dices. The special color protection solution is in favor of improving the color protection effect and shortening the color protection time, has obvious color protection and brittleness protection effects, and substantially reduces the fruit yellowing rate; and a blanching process is reduced, so energy consumed in the processing process is reduced, the hardness, the crispness, the color, the mouthfeel and other quality indexes of quick-frozen apple dices are improved, and the storage and shelf life of the above product is prolonged to 2 years, thereby the product has high hardness and good mouthfeel, pollution to environment is reduced, and the production efficiency of enterprises is improved.

The invention discloses an efficient inducing method of rhizoma coptidis hairy roots. The efficient inducing method specifically comprises the following steps: S1, sterile tissue culture seedlings areobtained; S2, explants are pre-cultured; S3, agrobacterium rhizogenes is activated; S4, the hairy roots are induced and sterilized; S5, liquid culture of the hairy roots is conducted; and S6, PCR detection of the hairy roots is conducted. By systematacially researching multiple factors influencing generation of the rhizoma coptidis hairy roots, certain methods for increasing the inducing rate ofthe hairy roots are explored, an efficient inducing system of the rhizoma coptidis hairy roots is built for the first time, and the inducing rate of the hairy roots reaches up to 88.7%; and the hairyroots obtained through the efficient inducing method are easy to breed and culture on a large scale, the potency of generating secondary metabolites is achieved, and the foundation for future development and utilization of the rhizoma coptidis hairy roots is laid.

The invention relates to the technical field of plant tissue culture, in particular to a Cattleya tissue culture propagation method, comprising the following steps: material selection, explant pretreatment, explant disinfection, induction culture, proliferation culture, strong seedling rooting and transplanting , Cattleya can be rapidly cultivated and propagated through the present invention, and the cultivated Cattleya seedlings have a high seedling rate, good quality, and strong adaptability after cultivation, and meet the requirements of commercialization in a short period of time by means of biotechnology. The demand for production provides technical support for the large-scale and industrialized cultivation of Cattelan. At the same time, the tissue culture propagation method has good disinfection effect and low browning rate, thereby greatly improving the seedling rate and work efficiency, and saving resources.

The invention belongs to the technical field of apple seedling cultivation, and discloses a method for rapid propagation of apple rootstock SH40, by adopting a culture medium of MS+6-BA0.6mg / L+IBA0.1mg / L+PVP500mg / L for primary culture , adopt MS+6‑BA0.8mg / L+IBA0.1mg / L to process subculture, adopt rooting medium 1 / 2MS+IBA1.3mg / L+melatonin 0.03mg / L for rooting culture, and then use pure The hardening and transplanting of sawdust realizes the rapid propagation of apple rootstock, which not only ensures a high survival rate, but also retains the excellent characteristics of the rootstock, providing a convenient method and way for the development and further expansion of the apple industry.

The invention discloses a method for increasing the squalene content in siraitia grosvenorii. The method comprises the following steps that S1, tissue culture of siraitia grosvenorii explants is conducted in a solid culture medium containing methyl jasmonate to obtain siraitia grosvenorii seedlings; and S2, the siraitia grosvenorii seedlings are cultivated, during cultivation, the surface of the siraitia grosvenorii seedlings which are pollinated for 10-20 d is sprayed by 50-400 [mu]mol / L of methyl jasmonate solutions until water dripping is formed on the surface. The methyl jasmonate are added in the process of siraitia grosvenorii tissue culture and cultivation, and high expression of a metabolic key enzyme gene of siraitia grosvenorii squalene is induced, so that the content of the siraitia grosvenorii squalene is quickly increased in a short time.

The invention relates to a combined method for preventing Phalaenopsis amabilis from browning in plant tissue culture, the adopted V31 (Phalaenopsis amabilis) is also called 'TaiLinhongtianshi', and is one of a red flower line of the Phalaenopsis amabilis. A culture medium containing natural additives is utilized for preventing the common browning phenomenon of the Phalaenopsis amabilis, so as to form the combined browning-prevention method. The method has high stability and high success rate, is green and environment-friendly, can be repeatedly used for production annually, and solves the problem that the existing method has low success rate and is not environment-friendly, thereby having great significance of real-world applications.

The invention discloses a method for novel variety culture and rapid tissue culture propagation of paphiopedilum and relates to the technical field of breeding and tissue culture of paphiopedilum. Themethod for novel variety culture and rapid tissue culture propagation of paphiopedilum comprises the following steps: performing artificial hybridization pollination, performing sterile seed sowing and seed germination, strengthening seedlings, promoting rooting, and transplanting test tube seedlings. The method is good in operability, high in application value, high in hybridization success rate, and very effective for excellent cross parents of paphiopedilum in flowering asynchronism; in the rapid tissue culture propagation process, a unique seed germination culture medium and a seedling strengthening rooting culture medium are used, so that the germination rate of paphiopedilum hybridization seeds is increased, seedlings can grow rapidly, and the seedlings are good in quality. The method for novel variety culture and rapid tissue culture propagation of paphiopedilum, which is disclosed by the invention, has the characteristics of being good in operability, high in application value, high in hybridization success rate, and the like, the problems that conventional novel variety culture and rapid tissue culture propagation process of paphiopedilum is low in germination rate, hardin seedling growth, long in propagation cycle, low in propagation coefficient, large in propagation difficulty, and the like, are solved, an effective way is provided for production of paphiopedilum seedlings, and industrial planting can be achieved.