8results about How to "Reduce browning rate" patented technology

The invention discloses a tissue culture and rapid propagation method for a fig male tree germplasm material, and belongs to the technical field of plant tissue culture and rapid propagation. The specific method comprises the following steps: (1) obtaining and disinfecting an explant; (2) primary culture of buds; (3) subculture multiplication of buds; (3) rooting culture; and (4) hardening seedlings and transplanting. According to the method, the optimal culture medium for tissue culture and rapid propagation of the fig male tree is obtained by combining different hormones and adjusting different hormone concentration ratios, and the differentiation and proliferation of buds of the fig male tree and the growth and rooting rate of seedlings are remarkably improved. In the explant treatment, tebuconazole carbendazim, 75% alcohol and sodium hypochlorite are adopted for the first time, so that the use of a highly toxic reagent mercury bichloride is avoided; cefotaxime sodium is added into a primary culture medium, so that the pollution rate of explants is reduced; the method is simple to operate, time-saving, labor-saving, safe and environment-friendly, and improves the inoculation quality and efficiency. The method is not limited by seasons, enlarges the excellent male tree population of the figs in a short time, and lays a foundation for cultivating new germplasm of the figs.

The invention provides a culture medium for reducing the content of broussonetia papyrifera esterase and a tissue culture method, and belongs to the technical field of broussonetia papyrifera tissue culture. The culture medium for reducing the content of the broussonetia papyrifera esterase provided by the invention comprises an induction culture medium, a proliferation culture medium, a differentiation proliferation culture medium and a rooting culture medium, wherein the induction culture medium is composed of an MS culture medium, 6-BA and IAA; the proliferation culture medium is composed of a 1 / 2 MS culture medium, NAA, KT and IBA; the differentiation and proliferation culture medium consists of a B5 culture medium, folic acid, TDZ, 2, 4-D, sucrose and agar; the rooting culture medium consists of an N6 culture medium, gibberellin 3, ethylene, IAA, cane sugar and carrageenan. The culture medium and the tissue culture method provided by the invention can promote more than 98% of adventitious buds to root, ensure that the survival rate is more than 98% after acclimatization and transplantation, not only can effectively reduce the content of lignin in paper mulberry tissue culture seedlings, but also cannot influence the normal growth of the paper mulberry tissue culture seedlings.

The tissue culture and rapid propagation method comprises the following steps: (1) carrying out sterile treatment and low-temperature pretreatment on a hemarthria procumbens seed; (2) carrying out germination culture on the hemarthria procumbens seeds treated in the step (1); (3) carrying out induced culture to grow calluses; (4) proliferation and differentiation culture; (5) rooting culture; wherein the low-temperature pretreatment specifically comprises the following steps: after sterile treatment, soaking the seeds in sterile water at 4 DEG C in a dark place for 12-24 hours, and carrying out low-temperature pretreatment; in the step (3), the induction culture medium is prepared from 0.5 mg / L to 0.8 mg / L of MS + 2, 4-D, 0.1 mg / L to 0.5 mg / L of 6-BA, 0.01 mg / L to 0.05 mg / L of BR and 0.5 mg / L to 1.5 mg / L of AC. According to the method, the callus induction rate is greatly increased, meanwhile, the browning rate of the callus is remarkably reduced, the callus induction rate can reach 75% or above, and the browning rate of the callus is reduced to 5% or below.

The invention discloses a method for removing viruses and endogenous and exogenous bacteria from small yellow ginger, and relates to the technical field of virus-free seedling cultivation, and the method comprises the following steps: S1, selecting ginger blocks which grow robustly and fleshy and are free of damage and plant diseases and insect pests as explant materials, and placing the ginger blocks in a dark environment at 28-30 DEG C for accelerating germination; s2, when the ginger buds grow to 2-3cm, selecting healthy and full ginger buds, cutting off the ginger buds, washing the ginger buds with running water, and disinfecting the ginger buds; s3, under a sterile condition, transferring the disinfected ginger buds into a primary culture medium, screening out the ginger seedlings with bacteria by adopting a high-low temperature method, and ending culture when the ginger buds grow 1-3 coleoptiles; s4, stripping leaf coleoptiles of the screened healthy and pollution-free ginger buds, cutting off 1-3mm stem tips from growing points, and transferring the stem tips into a clumpy bud induction culture medium for multiplication culture at the culture temperature of 20-25 DEG C and the illumination intensity of 2500 LUX for 8-15 days; s5, the clustered seedlings obtained after proliferation are cut into single seedlings from the base parts, the single seedlings are placed in a rooting culture medium, and virus-free plants are obtained and cultured for 30-45 d at the culture temperature of 22-25 DEG C and the illumination intensity of 3000 LUX.

The present application relates to a kind of passion fruit explant brown prevention tissue culture medium and its tissue culture method, it is related to passion fruit tissue culture technical field.The present application provides a kind of passion fruit explant brown prevention tissue culture medium, the tissue culture medium includes the following components:MS culture medium, passion fruit juice, 6-BA, IBA, sucrose, agar.The present application is improved by culture medium, explant low-temperature treatment and explant secondary disinfection, and the pollution rate of explant, browning rate is reduced, improves the survival rate of passion fruit explant in the process of tissue culture, overcomes the shortcoming that browning mortality is high in the process of passion fruit tissue culture, improves the tissue culture efficiency of passion fruit.

The application discloses a tissue culture seedling raising method of Yunnan polygonatum sibiricum, and belongs to the technical field of biology, and comprises the following steps: S1, treatment of explants: selecting clean Yunnan polygonatum sibiricum tubers with buds, and culturing the tubers under high-temperature stress at 40-45 DEG C for 12-24 h, and then directly transferring the tubers to low-temperature stress treatment at 2-4 DEG C for 24-36 h; S2, induction of callus: taking the treated tuber buds as explants, inoculating the explants into a callus induction culture medium after disinfection, and dark culture for 10-12 days; the callus induction culture medium comprises 1 / 2MS+2,4-D 1-2 mg / L, 6-BA 2-3 mg / L, NAA 0.3-0.6 mg / L and 1wt%-3wt% cactus juice; S3, induction of bud differentiation and induction of rooting. The tissue culture seedling raising method of Yunnan polygonatum sibiricum can effectively reduce the browning rate in induction of callus of Yunnan polygonatum sibiricum with tuber buds as explants.

The present application relates to the processing technology field of Rhizoma Polygonati, in particular to a low-temperature tumbling system and process for directional softening and browning inhibition of Rhizoma Polygonati fibers; the system comprises a tumbling barrel, a collection box, a cooling circulation module and an ultraviolet-ultrasonic synergistic treatment unit. The tumbling barrel breaks Rhizoma Polygonati cells and releases juice during rotation, the juice is filtered by the collection box, cooled to 0-10 DEG C by a cooling device, and then recycled and sprayed onto the surface of Rhizoma Polygonati to realize dynamic temperature control; the ultraviolet lamp inhibits the reproduction of microorganisms, the ultrasonic wave destroys the activity of polyphenol oxidase, and the low-temperature environment blocks the browning reaction; the process realizes directional softening of Rhizoma Polygonati fibers under low-temperature conditions by alternating tumbling and standing, synergistically controls the softening rate of fibers and the inhibition efficiency of enzyme activity, and significantly reduces the browning risk. The system takes into account the quality and processing efficiency of Rhizoma Polygonati, is suitable for the industrialized processing of different varieties such as Rhizoma Polygonati and Rhizoma Polygonati, and can be widely applied to the processing technology field of Rhizoma Polygonati.

This invention discloses a method for tissue culture of *Anoectochilus pingbianense*, comprising: selecting wild *Anoectochilus pingbianense*, removing leaves and roots, rinsing, cutting stem segments, soaking in alcohol and rinsing with sterile water, soaking in sodium hypochlorite and rinsing with sterile water again to obtain treated stem segments; inoculating the treated stem segments into an induction medium for culture; inoculating the obtained stem segments into a rooting medium for culture; and inoculating the obtained stem segments into a tissue culture seedling medium for culture to obtain tissue culture seedlings with complete roots, stems and leaves. This invention solves the problems of severe browning and long propagation cycle in existing *Anoectochilus pingbianense* tissue culture, which cannot meet production needs, greatly reduces possible contamination during tissue culture, reduces the browning rate of explants, and shortens the growth cycle of tissue culture seedlings.