Trichoderma asperellum and application thereof

A technology of Trichoderma and Acanthospores, applied in application, fungicides, fungi and other directions, can solve the problems of complicated operation and secondary infection of bacteria, and achieve the effect of reducing morbidity and promoting growth.

Active Publication Date: 2019-05-14
CHONGQING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Apple rot pathogen Phytophthoraplurivora is a new disease of apple trees. Infected fruit trees need to completely scrape off

Method used

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  • Trichoderma asperellum and application thereof
  • Trichoderma asperellum and application thereof
  • Trichoderma asperellum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0038] Embodiment 3 Trichoderma asperellum TC01 metabolic volatiles inhibit the pathogenic bacteria

[0039] TC01 and the tested pathogenic bacteria (tea tree anthracnose pathogen or apple rot pathogen) were simultaneously expanded and cultivated for three days to make their growth potential consistent. Adopt butt-button culture method, use 5mm puncher to get fresh mycelium block respectively at the colony edge of TC01 bacterial strain and test pathogen, place the mycelium block respectively in the center of 9cm petri dish containing 20ml PDA, mix TC01 and test The bottom of the pathogenic bacteria dish is double-buttoned, the bottom of the pathogenic bacteria dish is on the bottom, and the bottom of the TC01 dish is on the top. Wrap the treatment with parafilm. Each treatment is repeated 3 times. The treatment with pathogenic bacteria on both sides is used as a blank control. In a constant temperature incubator at 28°C After cultivating 7d, calculate bacteriostasis rate, bact...

Embodiment 4

[0040] Example 4 Trichoderma asperellum TC01 fermented liquid prevents and controls anthracnose of tea trees and promotes growth of tea seedlings

[0041] Preparation of fermentation broth: After activating the strain, inoculate it on a cellophane-lined PDA plate and culture it at 28°C for 2 days, collect mycelium (about 0.5 g) and stir it to grind it, and inoculate it into 100 mL of PDB medium (200 g of potato, 20 g of glucose) , distilled water 1000ml) in a 250mL Erlenmeyer flask, 120r / min, 28°C constant temperature shaking culture for 7d, filter with 4 layers of sterile gauze to obtain a fermentation broth.

[0042] Routine management of potted annual tea seedling plants in the field, set up 4 groups of experiments:

[0043] (1) CK: root irrigation with sterile water;

[0044] (2) B: the roots are irrigated with sterile water, and the leaves are inoculated with anthracnose pathogenic bacteria;

[0045] (3) BT: TC01 fermented liquid (100 times) irrigates roots to process t...

Embodiment 5

[0061] Example 5 The growth-promoting effect of Trichoderma asperellum TC01 conidia suspension on tea seedlings and the prevention and control effect on tea tree anthracnose

[0062] Preparation of conidia suspension: inoculate Trichoderma asperellum TC01 strain on PDA medium, and culture in the dark at 28°C for 7 days to obtain colonies of TC01 strain; add 20ml of sterile water to the petri dish, scrape and wash the meristems on the surface Spores were filtered with 4 layers of sterilized gauze to obtain a conidia suspension, and the concentration was adjusted to 1×10 7 conidia / ml.

[0063] Pot test: annual tea seedling plants, leaves were inoculated with anthracnose pathogenic bacteria, and TC01 conidia suspension was used to carry out regular root irrigation treatment, 3 repetitions were set, and the root irrigation amount in each pot was 20ml (concentration is 1 × 10 5 conidia / ml), the root irrigation treatment was carried out every 7 days for a total of 4 times; clean wa...

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Abstract

The invention discloses a Trichoderma asperellum TC01. The Trichoderma asperellum TC01 is collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC NO. 3.19218. The Trichoderma asperellum TC01 has the remarkable inhibiting effect on a tea-tree anthracnose pathogenic bacterium Colletotrichum gloeosporioides and an apple valsa canker pathogenic bacterium Phytophthora plurivora, and antibacterial substances generated by the Trichoderma asperellum TC01 can also inhibit growth of the tea-tree anthracnose pathogenic bacterium and the apple valsa canker pathogenic bacterium. The trichoderma asperellum can also promote growth of tea seedlings, and is a strain having the good environmental safety, the biological control capacity and the growth promotion function.

Description

technical field [0001] The invention relates to the technical field of microorganisms and biological fertilizers, in particular to a Trichoderma aculeatus and its application. Background technique [0002] Trichoderma (Trichoderma spp.) widely exists in soil, plant rhizosphere, leaf surface, bark and litter. It has strong growth and reproduction ability. It produces a variety of antagonistic metabolites, rich enzymes and growth-promoting factors, and is one of the most widely used probiotics. [0003] At present, domestic research on Trichoderma mainly focuses on T. harzianum, T. atroviride, T. virens, T. reesei and T. longibrachiatum. T. asperellum is a newly recorded species of Trichoderma in my country in recent years, and its development and utilization is still in its infancy. [0004] Tea tree is a perennial crop, which is mainly harvested from young shoots; the occurrence of tea tree anthracnose can cause a large number of tea leaves to fall, which in turn weakens t...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
Inventor 商靖徐泽唐敏林强陈世春江宏燕王晓庆
Owner CHONGQING ACAD OF AGRI SCI
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