23 results about "New disease" patented technology

The invention discloses application of TBR2 protein as well as a coding gene and a molecular marker thereof in resisting tomato brown wrinkled fruit virus diseases, and belongs to the technical field of gene engineering. According to the invention, a novel anti-disease gene TBR2 for resisting the tomato brown wrinkled fruit virus disease is identified on a chromosome 4 of tomatoes; the disease-resistant gene has stable disease resistance, and the resistance of the variety to the tomato brown crinkled fruit virus disease can be obviously improved by backcrossing and transbreeding the disease-resistant gene into a sensitive cultivar. Based on the anti-disease gene TBR2, the invention further develops molecular markers related to the tomato brown wrinkled fruit virus disease resistance, and the molecular markers can be used for identifying plant materials resistant to the tomato brown wrinkled fruit virus disease and cultivating plant varieties resistant to the tomato brown wrinkled fruit virus disease. The method is of great significance in accelerating the breeding process of disease-resistant tomatoes.

The invention discloses a training method, system and device of a disease auxiliary diagnosis model and a medium. The method comprises the steps of preprocessing local multi-modal medical data of each mechanism; performing adversarial training on the multi-modal medical data through a generator and a discriminator to generate a high-fidelity synthetic sample; updating gradient parameters of the disease auxiliary diagnosis model based on the synthetic sample; the gradient parameters are encrypted and uploaded to a federal center server; the server aggregates the gradients and issues the gradients to each mechanism; and generating a disease auxiliary diagnosis model after iterative optimization. According to the method, scarce samples are supplemented through multi-modal data standardization and adversarial generation, cross-institution privacy protection cooperation is realized in combination with federated learning and a differential privacy technology, a global optimal model can be constructed without sharing original data, the model diagnosis accuracy and generalization ability are improved, the problem of data imbalance is effectively relieved, and the user experience is improved. And clinical auxiliary diagnosis and medical scientific research requirements are met.

Provided is a use of genes OsADR1 and OsTIR in enhancing broad-spectrum disease resistance in rice. The genes OsADR1 and OsTIR can enhance resistance in rice against diseases such as rice blast and sheath blight, and thus play a valuable role in the development of new disease-resistant rice varieties.

The invention discloses a culture method based on posthypoxia gastric intima separation treatment of freshwater white pomfret, and relates to the technical field of aquaculture and disease prevention and control. The method comprises the following steps: step 1, identifying hypoxia symptoms; 2, medicine compatibility feeding is carried out; 3, regulating and controlling the water body environment; 4, breeding environment management; and step 5, continuous monitoring. According to the invention, the food maternal, the endothelium corneum gigeriae galli and the pipemidic acid are combined for the separation treatment of the endogastric membranes of the aquatic animals for the first time, the thinking limitation of traditional disease prevention and treatment is broken through, the traditional Chinese and western medicine theories are organically combined, and a new disease prevention and treatment thought is provided for the aquaculture industry; compared with a traditional pure antibiotic treatment or water quality improvement method, the scheme is high in pertinence and directly aims at a core pathological link of gastric intima separation after hypoxia; the comprehensive treatment effect is good, and meanwhile, anti-infection, mucous membrane repair and function regulation are considered; and the used medicines are all common medicines, have small side effects and low residues, and meet the safety requirements of aquatic products.

The invention discloses a tobacco cytochrome c1 subunit and application thereof in plant disease resistance. Belongs to the technical field of gene engineering. The cDNA (complementary deoxyribonucleic acid) nucleotide sequences of the tobacco NtCytc1-1a / 1-1b / 1-2a / 1-2b / 1-2c / 1-2d gene are as shown in SEQ ID NO: 1-SEQ ID NO: 6. The function loss of the protein coded by the tobacco NtCytc1-1a / 1-1b / 1-2a / 1-2b / 1-2c / 1-2d gene leads to mROS outbreak, disease resistance enhancement and GmMEKK1 response enhancement of the plant. The method has a guiding effect on cultivation of new disease-resistant varieties of crops.

This invention discloses the application of TaCYP51H37 protein and related biomaterials in regulating plant stem base rot resistance. This invention constructs a transgenic... TaCYP51H37 wheat and TaCYP51H37 Silent wheat, through identification of its resistance to stem base rot, was found to show that, compared with the recipient control, it was more susceptible to *Fusarium graminearum* infection. TaCYP51H37 The disease severity index of wheat was significantly reduced, while the aboveground length, aboveground fresh weight, and aboveground dry weight were all significantly increased. TaCYP51H37 The phenotype of silent wheat was the opposite, with a disease severity index significantly higher than that of the control plants, and significantly lower aboveground part length, aboveground fresh weight, and aboveground dry weight. This invention is the first to discover that the TaCYP51H37 protein positively regulates plant resistance to stem rot, which is of great significance and application value for improving plant disease resistance and cultivating new disease-resistant plant varieties.

The invention belongs to the technical field of artificial intelligence, and discloses a chronic disease co-disease new risk assessment method, system, device and medium, and the method comprises the steps: employing a time sequence ternary group to model a disease new path, introducing a multi-disease co-existence coefficient to quantify the new risk, and carrying out the grading, learning disease network representation by adopting a relational graph convolutional network (R-GCN), and predicting a new risk; secondly, designing a cross-time window joint training strategy, performing hot start training on the model by using embedded representation of historical data, expanding representation information of an encoder for incremental data, fusing the representation information into a disease network, and integrating data to iteratively optimize the model; according to the method, through systematic modeling of the patient medical history data and the disease association network, the potential law of the new occurrence of the common disease is disclosed, and an analysis tool and direction guidance are provided for subsequently exploring the common disease mechanism of a specific disease and identifying a high-risk evolution mode.

The application belongs to the technical field of plant disease resistance breeding, and provides application of GmbZIP111 protein and / or its coding gene in improving resistance of soybean to pythium. GmbZIP111 The gene positively regulates resistance of soybean to pythium root rot, overexpression of the gene can significantly inhibit pythium infection of soybean, and knockout of the gene leads to reduced resistance. GmbZIP111 The gene has important theoretical significance and application value for analyzing the mechanism of soybean resistance to pythium root rot and cultivating new disease-resistant varieties.

This invention discloses a molecular marker associated with a major QTL for resistance to Fusarium wilt in cowpea, its primers, and their applications, belonging to the field of plant molecular breeding technology. The associated KASP-type molecular marker 3_00160 is located at 46659973 bp on chromosome 6. This marker can be used to amplify test materials, and the genotype can be used to determine whether the sample carries the allelic variant controlling Fusarium wilt resistance. The KASP marker primer combination includes the following three sequences: Primer1 (sequence 2), Primer2 (sequence 3), and Primer_Common (sequence 4). Using the molecular marker and primers provided by this invention, rapid screening of major QTLs for resistance to Fusarium wilt in cowpea can be achieved, which is of great significance for breeding new disease-resistant varieties.

The invention discloses a corn disease-resistant molecular marker and application. The nucleotide sequence of the corn disease-resistant molecular marker ZmCRK10Type B is as shown in SEQ ID NO: 3. The gene ZmCRK10 disclosed by the invention encodes cysteine-rich receptor kinase, and a T03 transcript of the gene is over-expressed, so that the disease resistance of corn is improved. The gene provides valuable resources for cultivation of maize disease-resistant varieties in a molecular breeding mode. Resequencing of associated populations subsequently finds that when a large-fragment structure variation exists in a corn ZmCRK10 promoter region and a transposon with the length of about 63.3 kb exists, the expression of a T03 transcript of ZmCRK10 can be remarkably improved, the resistance of multiple diseases of corn can be promoted, and the ZmCRK10 gene can be used as a corn disease-resistant molecular marker to cultivate a new variety of disease-resistant corn.

The application discloses TBR2 protein and an application of an encoding gene and a molecular marker thereof in resisting tomato brown rugose fruit virus disease, and belongs to the technical field of genetic engineering. TBR2 A new disease-resistant gene of the tomato brown rugose fruit virus disease is identified on the 4th chromosome of the tomato TBR2 ; the disease-resistant gene has stable disease resistance; backcrossing and transformation of the disease-resistant gene into a sensitive cultivar can obviously improve the resistance of the cultivar to the tomato brown rugose fruit virus disease. Based on the disease-resistant gene TBR2 , the application also develops molecular markers respectively related to the resistance of the tomato brown rugose fruit virus disease; the molecular markers of the application can be used for identifying plant materials resistant to the tomato brown rugose fruit virus disease and cultivating plant varieties resistant to the tomato brown rugose fruit virus disease, and have important significance for accelerating the breeding process of the disease-resistant tomato.

The invention discloses a construction method of a fish nocardia disease pathogenic mechanism model, and belongs to the field of biotechnology and disease models. The invention aims to solve the technical problem that a deep causal mechanism is difficult to reveal due to the fact that microscopic dynamic interaction of pathogens and hosts cannot be observed in real time and in situ when an existing animal model and an in-vitro cell model are used for researching the disease. The method comprises the following steps: constructing a fish immune organ in an intelligent micro-fluidic chip integrated with a micro-actuator as a high-bionic-degree physical experiment platform; the living cell imaging system is used for collecting four-dimensional image data of the organoids in the nocardia infection process in real time. According to the method, through normal form transformation from passive observation to active intervention, the microscopic process of the disease can be reproduced in a high-fidelity manner and is regulated and controlled prospectively, so that a causal chain between key pathological events is deeply revealed, and a powerful intelligent research platform is provided for developing a new disease prevention and treatment strategy.

The invention provides an urban road disease automatic processing method and device based on a large model agent, and the method comprises the steps: obtaining disease report information, adding the disease report information to an existing disease file or building a new disease file through a super agent, and enabling each disease file to correspond to a disease and an autonomous agent; performing grading processing on diseases in the new disease file through an autonomous agent to obtain disease grades; when the disease level is that the disease needs to be treated, generating a disease work order; based on the plurality of work order distribution factors, sending the disease work order to the target object through the super agent; the disease maintenance information of the disease work order is obtained through the autonomous agent, the maintenance effect is evaluated based on the disease maintenance information, the maintenance effect evaluation result is obtained, and the service efficiency and the treatment capacity are remarkably improved.

The invention discloses hydrogel microspheres for inhibiting mitochondrial DNA release and improving osteoarthritis and application, and aims at the clinical dilemma that early and middle-stage osteoarthritis lacks specific treatment, the invention discovers that mechanical overload induced mitochondrial membrane potential reduction (from-180mV to-100mV) leads to abnormal opening of VDAC, miMOMP change and mtDNA outleakage, and the hydrogel microspheres can inhibit mitochondrial DNA release and improve osteoarthritis. The method is a key mechanism for steady-state imbalance of cartilage cells. On the basis, a Fe / BiOCl (at) AHAMA-GelMA dual response system is constructed, wherein the Fe / BiOCl nanosheet has positive charges (+ 25-30 mV) in a resting state and can target a mitochondrial inner membrane, and local negative potential (about-50 mV) is generated under mechanical stimulation to release electrons to recover membrane potential; the AHAMA-GelMA hydrogel microspheres are combined with amino groups on the surface of cartilage through aldehyde groups to realize spatial positioning, and a targeted mechanical overload area is recognized by utilizing the specificity of MMPs to gelatin. In-vivo and in-vitro experiments prove that the system can convert overload abnormal stress into repair electric energy, inhibit mtDNA outleakage, remarkably improve the mitochondrial function and relieve cartilage degeneration, and a new disease modification treatment strategy is provided for osteoarthritis.

This invention discloses a method and system for constructing crop disease models based on the coupling of multiple environmental factors, belonging to the field of smart agriculture and plant protection technology. The method includes: acquiring environmental data, soil data, crop data, and pathogen data; calculating the spore attachment success rate based on the pathogen data; dividing the spore infection process into four stages: dormancy, germination, germ tube, and infection spike stage, constructing an infection state machine model to maintain the spore stage state and calculate germination progress and cumulative infection values, thereby calculating the infection activity output; calculating water stress development correction coefficients, nutrient stress development correction coefficients, pesticide application pathogen mortality correction coefficients, and carbon dioxide content stress correction coefficients based on soil and crop data; and multiplying the spore attachment success rate, infection activity output, and multiple correction coefficients to obtain the disease risk value. This invention enables the reuse of a single model framework for multiple diseases, significantly reducing the development cycle and cost of new disease models.

The invention relates to the technical field of medical data processing and disease prediction, and discloses a disease risk prediction method based on data fusion, and the method comprises the steps: constructing a disease risk prediction model; collecting multi-source medical data; after performing different types of data processing on the multi-source medical data, inputting the multi-source medical data into a disease risk prediction model to perform disease risk prediction, and generating a disease risk prediction result; according to the method, targeted preprocessing is performed on the multi-source medical data, so that the quality and consistency of the data are improved, and a good foundation is laid for subsequent data fusion and prediction; meanwhile, a new disease risk prediction model is constructed, different types of data are processed by designing different input branches, and multi-source data fusion is performed in combination with an attention mechanism, so that deep association among the multi-source data can be fully mined, disease association information can be comprehensively captured, the accuracy and robustness of disease risk prediction are improved, and the accuracy and robustness of disease risk prediction are improved. And efficient and reliable decision support is provided for clinical disease risk assessment.

The invention provides an application of ecripara in the preparation of a cGAS-STING agonist (cGAS-STING agonist). Studies prove that the ekripara can obviously activate an STING signal channel in tumor cells, induce a downstream I-type interferon reaction and increase the infiltration level of T lymphocytes in tumor tissues. The invention provides a small molecule compound capable of activating an STING signal channel, provides a new disease application for ecrista, and provides a new possibility for development of drugs for enhancing anti-tumor immunity.

The invention discloses a KASP marker closely linked with a wheat scab resistance gene and application of the KASP marker, and belongs to the technical field of wheat molecular breeding. Two new gibberellic disease resistant sites are identified, CCCACCG / GCACAT nucleotide polymorphisms exist at the two sites, based on the gibberellic disease resistant sites, the KASP marker closely linked with the wheat gibberellic disease resistant gene is developed, different genotypes of wheat can be distinguished through PCR amplification and fluorescence signal detection, the molecular breeding efficiency is greatly improved, and the application prospect is wide. And a new germplasm material is provided for cultivation of a new disease-resistant variety of wheat.

The present application relates to the field of plants, in particular to application of GhCaM53 gene in improving resistance of plants to verticillium wilt. The protein encoded by the mutant of GhCaM53 gene has the sequence of the amino acid sequence shown in SEQ ID NO:3 or SEQ ID NO:4. The present application aims to analyze the ubiquitination modification characteristics of GhCaM53 protein and its function in cotton resistance to verticillium wilt, to clarify the immune response signal pathway mediated by it, to provide a new target gene (GhCaM53) and theoretical support for molecular breeding of cotton resistance to verticillium wilt, and to make up for the deficiency of the prior art in exploring new disease-resistant genes and key regulatory mechanisms.

The invention discloses a molecular marker related to cowpea fusarium wilt resistance major QTL (quantitative trait loci) as well as primers and application thereof, and belongs to the technical field of plant molecular breeding. A KASP type molecular marker 300160 related to the KASP type molecular marker 300160 is located at the position 46659973 bp on a chromosome 6, the marker can be used for amplifying a to-be-detected material, and whether a sample carries allelic variation for controlling wilt resistance or not is judged according to the genotype of the to-be-detected material. The KASP labeled primer combination comprises the following three sequences: a forward primer Primer1 as shown in a sequence 2, a forward primer Primer2 as shown in a sequence 3 and a universal reverse primer PrimerCommon as shown in a sequence 4. By utilizing the molecular marker and the primer thereof provided by the invention, rapid screening of the main effect QTL for resisting the fusarium wilt of the cowpea can be realized, and the molecular marker and the primer thereof are of great significance for breeding new disease-resistant varieties.

The application discloses a method for constructing a large fragment deletion mutant library of Magnaporthe oryzae, and belongs to the field of agricultural biotechnology.The method comprises the step of adding DEB to the protoplast of the Magnaporthe oryzae for culture and inducing the production of conidia.The application utilizes the chemical mutagen DEB to obtain the Magnaporthe oryzae mutant at a high frequency on a whole genome scale, and provides a new technical path for the functional genomics research of the Magnaporthe oryzae.The method provided by the application is helpful to systematically analyze the pathogenic mechanism, the infection process and the environmental adaptation regulation network of the Magnaporthe oryzae, and provides support for mining new disease-resistant targets and disease-resistant mechanisms.The method has the advantages of high efficiency, low cost and short cycle, and has wide application potential in the genetic research of plant pathogenic fungi.

The invention discloses a molecular marker of an interferon gene stimulating factor and application of the molecular marker. Specifically, the invention provides a molecular marker related to fish infectious spleen and kidney necrosis disease resistance, the marker is located at the 315th site of an interferon gene stimulating factor (STING) protein coding amino acid sequence, and the polymorphism of the marker comprises lysine (K), threonine (T), glutamine or arginine. It is found that when the molecular marker is an amino acid (such as K or T) which can be ubiquitinated, STING containing the molecular marker can be ubiquitinated by VP012R of ISKNV and cause degradation, so that ISKNV can escape from interferon reaction of a host, and the host is dead; on the contrary, when the site is the amino acid which cannot be ubiquitinated, the host can resist ISKNV infection through interferon reaction, so that disease resistance is shown. The molecular marker provided by the invention can be used as a target spot for screening disease-resistant individuals or breeding new disease-resistant strains.