339 results about "Gossypium hirsutum" patented technology

The invention discloses an upland cotton SNP marker and application thereof, and belongs to the field of upland cotton SNP marker development. The upland cotton SNP marker is obtained by developing a single-copy SNP marker of an upland cotton genome via chip hybridization and homologous sequence comparison, and picking out SNP markers in a linkage disequilibrium recession distance via linkage disequilibrium analysis. The SNP marker provided by the invention can be applied to analysis of genetic diversity and group structure, or to germplasm identification of upland cotton, is single-copy, and has no remarkable linkage disequilibrium among markers, so that the efficiency of detection, adopting the SNP marker, during analysis of genetic diversity, group structure or fingerprint identification can be greatly improved, and the work efficiency is higher.

The invention provides a high-yield cultivation method for planting a precocious upland cotton variety in a precocious cotton area, in order to solve the problem of greatly reduced output caused by the windstorm disaster in a southern Xinjiang precocious cotton area or the precocious and middle-mature varieties after late sowing is performed. Different from the traditional cultivation method for the precocious and middle-mature varieties, the high-yield cultivation method is characterized in that: 1) the sowing time is delayed for 10-25 days; 2) the sowing density is increased by 1000 strainsper mu; 3) topping is performed before and after July 20, the topping time is delayed for 8-12 days, the number of leaved fruit spurs is increased to 1-2 and the stem length is increased to 5-10cm; 4) after topping, the chemical regulating times is changed into two from one, the elongation of the top fruit spurs is boosted, the number of the coped buds and the bud yield are increased; and 5) the pre-frost yield rate is increased from 95% to above 98%. According to the high-yield cultivation method provided by the invention, the output of cotton is not reduced by the after-culture or late-culture, the output of pre-frost lint is slightly increased, the level of the lint is increased and the income of a cotton planter is increased.

The invention discloser SSR markers lined with major genes of cotton fiber strength, which is obtained by the following steps: generating F2 and F2:3 populations by using a cotton search 41 line sGK9708 selected from cultivated varieties of gossypium hirsutum and a high quality line 0-153 of gossypium hirsutum as parents; allowing generation within the family of the F2:3 population to self cross till the F2:6 generation, performing within-family individual selection of the F2:6 generation once, and planting two generation till F6:8; performing polymorphism screening of the parents by using SSR primers and creating an RIL population linkage map; and performing the multi-environment major QTL screening of the cotton fiber strength to screen 6 QTLs of a cotton fiber strength character from line 0-153, wherein 5 QTLs are multi-environment stable QTLs and are FS1 linkage marker NAU2119330, FS2 linkage markers BNL2572125, BNL1064110 and DPL0874210, FS4 linkage markers are NAU1048250 and NAU2627350, and FS5 linkage markers BNL1421200 and NAU2730450. The SSR markers lined with the major genes of the cotton fiber strength are screen from high fiber quality materials and used as molecular markers to perform early auxiliary selection on a DNA level to improve the selection efficiency of the cotton fiber strength.

The invention relates to a molecular marker for assisted selective breeding of upland cotton with greensickness resistant traits, belonging to the technical field of genetic breeding of cotton. Three SSR (Simple Sequence Repeat) markers for assisted selective breeding of the upland cotton resisting greensickness can be obtained by constructing a molecular marker genetic linkage map in upland cotton seeds to carry out QTL (Quantitative Trait Locus) positioning on the greensickness resistant traits and selecting remarkable SSR markers entering a linkage group section and the SSR makers linked with disease-resistant QTLs to screen different upland cotton plant systems. The three markers comprise marker primers and a primer combination and are suitable for greensickness-resistant molecular marker-assisted selective breeding of the upland cotton. The plant systems resisting the greensickness can be obtained by using the obtained molecular marker to carry out greensickness-resistant molecular marker-assisted selection on different upland cotton plant systems.

The invention relates to SNP molecular markers related to fiber strength of an upland cotton 25# chromosome. The invention belongs to the technical field of cotton molecular breeding and discloses the SNP molecular markers related to fiber strength of the upland cotton and detection and application thereof. The SNP molecular markers are obtained by taking a stable RIL group of cotton as a material by means of a genome re-sequencing method. The SNP markers disclosed by the invention are used for molecular marker-assisted selection, so that the breeding period can be greatly shortened, and the breeding efficiency of the cotton fiber strength can be increased.

The invention discloses an astopic promoter of cotton fiber with 1037bp from -634 to -543 as core area, which is characterized by the following: constructing the plant expressive carrier with promoter; transmitting the gene into tobacco and cotton; dyeing GUS tissue; displaying expressive activity of exosperm superiority through enzyme activity and GFP fluorescent fiber detection; possessing expressive astopy of fiber in the land cotton.

The invention relates to a method for breeding an upland cotton intervarietal hybrid, and belongs to the technical field of plant breeding. Multi-parents are crossbred through mixed pollination, and filial generation excellent plants are crossbred through mixed pollination to breed female parent line 29-8. The male parent 203-16 line is selected and bred through multi-parent hybrid, excellent plant mixed pollination hybrid, and combination of techniques such as identification of verticillium wilt disease and verticillium wilt hybrid disease nursery, space-flight induction and so on. The female parent line 29-8 and the male parent 203-16 line are prepared into a hybrid Ning 2058. The hybrid Ning 2058 belongs to middle-early ripen variety, the growth season is about 120 days, the plant height is 98.60 cm, the node position number of fruit branch is 6.38, the fruit branch number of single plant is 11.96, the boll number of the single plant is 22.66, the boll number per mu is 65,900, the single boll weight is 6.95g, the seed index is 10.88g, the gin turnout is 41.17 percent, the pre-frost lint percentage is 89.23 percent, and the hybrid is suitable to be planted in various cotton areas in Tianjin City.

The invention discloses gossypium hirsutum GhB2 protein as well as an encoding gene and application thereof. The GhB2 protein provided by the invention is as shown in (1) or (2): (1) proteins as shown in SEQ ID No. 2; (2) proteins obtained through substitution and/or deficiency and/or addition of one or more amino acid residues on amino acid sequences shown in the SEQ ID No. 2, and with the same functions. According to the gossypium hirsutum GhB2 protein and the encoding gene and application thereof disclosed by the invention, sequencing of a transcription group of Chinese planted cotton KV3 is used as a foundation, cotton GhB2 genes are cloned, typical DCD ultra-protein family genes are obtained, procedure death of cells is mediated, through gene silencing and overexpression analysis, the effects of the gossypium hirsutum GhB2 protein in verticillium wilt resistance for Gossypium hirsutum are discussed, and candidate genes are provided for subsequent cultivation of disease-resistant varieties through genetic engineering means.

The invention relates to a cotton drought resistant gene GhEXP1 and applications thereof, and belongs to the biology technical field. The related gene GhEXP1 is a cotton expansin gene and is reported for the first time. The gene is cloned from an upland cotton draught resistant species (Ao 3503), and through RT-PCR analysis, people find that the expression of GhEXP1 is up-regulated after a drought treatment. The drought resistant performance of strains containing silenced GhEXP1 gene is obviously reduced. The living rate of GhEXP1 transgene arabidopsis plants is 80% after a 30-day drought treatment; while the living rate of referential wild arabidopsis is 10%; and the result shows that the GhEXP1 gene can prominently improve the drought resistant performance of plants. The GhEXP1 gene is advantageously used for improving the plant drought resistance and has a wide application prospect.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a cotton calcium-dependent protein kinase GhCPK33 gene for regulating the verticillium wilt resistance of cotton, and applications thereof. According to the present invention, the calcium ion-dependent protein kinase GhCPK33 gene with the characteristic of verticillium wilt induced expression isscreened by inoculating Verticillium dahliae in Gossypium hirsutum Linn and analyzing RAN-Seq data at different time points, wherein the nucleotide sequence of the gene is represented by SEQ ID NO:1,and the protein sequence encoded by the gene is represented by SEQ ID NO:2. According to the present invention, through the VIGS system, the corresponding gene in cotton is silenced, Verticillium dahliae is inoculated, and the function of the gene in the verticillium wilt resistance of cotton is quickly identified; and the isolated gene provides the negative regulation effect in the verticillium wilt resistance of cotton, and by silencing the gene, the verticillium wilt resistance of cotton can be significantly improved.

The invention relates to a cotton continuous cropping high yield cultivation method, aiming at solving the technical problem of overcoming continuous cropping obstacles to achieve high yield cotton. In the invention, sea-island cottons or land-sea hybrid cottons which are highly resistant or immune to vascular bundle disease verticillium wilt and fusarium wilt are taken as rootstocks; conventional upland cottons variety with good affinity to the rootstocks are taken as scions; grafting is carried out during seedling stage; the grafted seedlings are placed in the environment avoiding light direct radiation with the temperature of 25-35 DEG C and the relative humidity of 85-95 percent for cultivating 5-10 days, and then are transplanted to a field with the density of 12000-18000 plants/hm2; and conventional field management is carried out after removing surplus buds on cotyledonary node position. The cotton plants formed through grafting has the advantages of high stress resistance and yield, great growth vigor in the continuous cropping cotton field, and excellent quality; moreover, by selecting the rootstocks and the scions with good affinity during cultivation, the grating survival rate is high, the costs on cultivation and improved varieties are low, and the popularization is easy.

The invention discloses a culture medium and a kit for preparing embryonic callus of cotton and applications of the culture medium and the kit. The culture medium comprises a callus induction medium, an embryonic callus induction medium and an embryonic callus proliferation medium, wherein the callus induction medium is an MSB medium into which 0.1mg/L of KT, 0.05mg/L of 2,4-D and 1.5g/L of gelrite are added; the embryonic callus induction medium is an MSB medium into which 1.9g/L of KNO3 and 2.0g/L of gelrite are added; the embryonic callus proliferation medium is an MSB medium into which 1.9g/L of KNO3, 0.1g/L of asparaginate, 0.1g/L of glutamine and 1.8g/L of gelrite are added. The culture medium disclosed by the invention is used for preparing embryonic callus of cotton, especially upland cotton and has the advantages that the initiating rate and the embryonic callus occurrence rate can be remarkably increased, the occurrence of abnormal embryos is reduced and the proliferation amount of the embryonic callus is very large.

The invention provides a method for breeding upland long-stapled cotton, which specifically comprises the following steps: carrying out the artificial cross between Xiang9520 selected as a female parent and Beiersinuo selected as a male parent according to the scientific breeding procedure; carrying out the modified backcross between the F1 generation as a male parent and Xiang9520; carrying out the multi-generation self-cross until the characters are stable; further selecting the high-grade individual-plant material from the field and carrying out the comparative test of strains to select a high-grade and high-yield strain Xiang309 with stable characters; sending the Xiang309 to space by a space ship, and further carrying out the systematic breeding process in the disease nursery after the Xiang309 is returned to the ground; and sampling and checking to obtain a over-length fiber variant individual plant, i.e., the upland long-stapled cotton named as Xiang9136. The invention has the following advantages: the technical route is advanced; the length of the bred upland long-stapled cotton reaches the length limit; and attaching importance to the high-yield selection, the single-boll weight of the Xiang9136 is 5.1g, the lint percentage thereof reaches 43.1%, the yield of unginned cotton per mu reaches 240kg, and the yield of ginned cotton per mu reaches more than 103.5kg.

The invention discloses a breeding method of a cotton cytoplasmic male sterile line. The method comprises the steps of hybridizing cotton Thurber's cotton (G.thurbtri) and upland cotton (G.hirsutum), selecting a sterile plant from filial generation; then carrying out backcross on the sterile plant, peach and an insect resistant material 6001, thereby obtaining the cotton cytoplasmic male sterile line after 12 generations of male sterile backcross transformation for at least seven years. The cotton cytoplasmic male sterile line screened and cultivated by the method disclosed by the invention is consistent in agronomic trait, stable and through in incompatibility, insect-resistant, and disease-resistant; the restorer recovery rate is 100%; the outcrossing rate is 91.2%; and the breeding method has wide development prospect and better utilization value.

The invention belongs to the technical field of plant gene engineering, in particular to a method of creating a high-oil cotton material by using a cotton GhPPC gene. The invention relates in particular to the gene GhPPC cloned from upland cotton, and functional verification and an application of the gene. Part of a conservative cDNA (complementary deoxyribonucleic acid) sequence of the obtained encoding gene is as shown as SEQ ID NO: 1 (sequence identifier number 1) in a sequence table. The invention further discloses an application of the gene GhPPC in genetic modification of the upland cotton, and a cottonseed oil content of the upland cotton can be increased by regulating expression of the cloned gene.

The invention relates to the field of cotton planting, in particular to a cultivation method of southern XinJiang film-free cotton. The method comprises the steps of choosing early-maturing upland cotton varieties for use, wherein the cotton varieties mature within 120 days under the film-mulching cultivation condition and mature within 135-140 days under the film-free cultivation condition; conducing fertilization and ploughing for sowing, wherein the planting density is 240,000-255,000 plants.hm<-2>; when cotyledons stretch flat, conducting continuous chemical regulation twice, wherein the usage amount of mepiquat chloride is 1.4-1.6 g.hm<-2> and 0.8-1.2 g.hm<-2> respectively, and the usage amount of mepiquat chloride is 4.3-4.8 g.hm<-2> after topping is conducted; conducting water and fertilizer management and disease pest and weed prevention and control in a growing period. According to the cultivation method of the southern XinJiang film-free cotton, the cotton does not need to be covered with mulching film, and thus white pollution caused by the mulching film is prevented; the specific early-maturing upland cotton varieties are selected and adopted, by adding a proper amount of base fertilizer and with the assistance of chemical regulation and water and fertilizer management, sufficient nutrients are provided for all stages of growth of the cotton, later maturity, yield reduction and quality lowering caused by later sowing are overcome in the cultivated cotton, and thus the yield is steadily increased, and the quality is gradually improved.

The invention discloses a kind of textile weaved by dogbane, cotton and leca covering yarn, the invention provides a kind of textile which has transverse bidirection elastane, has no stimulation to skin, cold-proof, ventilation is good, the hygroscopic is excellent, the elastane is large, at the same time, which also has decompression and blood-fat releasing function. The leca fiber is covered by the dogbane fiber and cotton, the weight percentage of dogbane fiber and cotton fiber are 20-50%, 50-80%, the weight of the leca fiber accounts for 10-12% in the textile. The cotton is natural color cotton or ground cotton, or pure natural long-fibred cotton. There also has a negative ion layer on the product, which is made up of following material with following weight percentage: calcium zeolite 30-45%, serpentine 20-45%, peridot 25-35%.

The invention discloses a development and application of an SSR (simple sequence repeats) marker for identifying prematurity of upland cotton. The invention provides a primer pair for identifying or assisting identification on prematurity of upland cotton. A primer pair of a following DNA (deoxyribonucleic acid) fragment is obtained through amplification, and the DNA fragment is obtained through PCR (polymerase chain reaction) amplification upon primer pairs of sequence 1 and sequence 2 in a sequence table by taking cotton genome DNA as a template. According to the development, a molecular marker within a target section is developed for a QTL (quantitative trait loci) site (qFT-D3-3) positioned on D3 chromosome of upland cotton, an SSR marker which is tightly interlinked with the target QTL is obtained, the correlativity with prematurity of cotton is marked through multi-group verification, then the SSR marker can be applied to selective application of prematurity of cotton, and significant resources for auxiliary selective breeding of molecular markers for premature cotton varieties are provided.

The present invention provides an ornamental cotton hybrid seed production method, a short season cotton conventional material or a short season cotton HB safflower marked material is used for hybridization with upland cotton material T586, by hybridization breeding, stable lines carrying different traits are bred; the lines are transformed into sterile lines by use of an upland cotton cytoplasmic sterile line material as female parent and the bred stable lines carrying different traits respectively as male parent; the obtained sterile line carrying target trait is selected as female parent, sea island cotton sea 7124 or upland cotton HB is selected as male parent, hybridized combination is configured, and the generation F1 is ornamental cotton. The method can shorten the breeding cycle of the ornamental cotton, and broaden the ornamental cotton parental origin. The performances of the ornamental cotton in planting are: sterility, and only flowering without balling and opening of bolls, nutrient demand is relatively less, the cost of cultivation is reduced, and the purity of the ornamental cotton can be ensured because no seed is left for re cultivation.

The invention relates to cotton flowering locus T GhFT and a vector, a construction body, a cell and polypeptide thereof, in particular to cotton flowering locus T and application thereof. In the invention, the flowering locus T GhFT1 of upland cotton mainly cultivated in Xinjiang is cloned, and the adjustment effect of the gene on the flowering of plants is researched. The invention also relatesto a vector, a host cell and a plant which comprise the gene. Due to the adoption of the gene, the flowering time of the plant can be advanced or the shape and the property of the plant can be improved.

The invention discloses an upland cotton GhMADS22 protein, and a coding gene and application thereof. The protein GhMADS22 has one of the following amino acid residue sequences: 1) amino acid residue sequence disclosed as SEQ ID No.2 in the sequence table; and 2) 1) derived protein related to plant photosynthetic efficiency, which is subjected to substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid residue sequence disclosed as SEQ ID No.2 in the sequence table. The protein and coding gene thereof can be used for cultivating cotton with advanced reproductive growth, higher planting density or increased number of secondary bolls, and enhancing the cotton yield, thereby laying foundation for cultivation of transgenic plants.

The invention belongs to the technical field of plant gene engineering and particularly relates to an accurate and efficient editing method of an upland cotton genome. A pRGEB32-GhU6.7-NPT II vector containing a cotton endogenous promoter pGhU6-7 is modified, original Cas9 protein is replaced with LbCpf1 protein, and a vector GhRBE3 with editing capacity in cotton is constructed. GhCLA is selected as a target gene to verify application of GhRBE3 in cotton. One target is designed, a Cpf1 gene editing system is imported into the cotton genome by agrobacterium tumefaciens mediated genetic transformation, Sanger sequencing and high-throughput sequencing are performed on transgenic plants to detect the editing efficiency and the off-target effect of the method in the allotetraploid cotton genome. The method has good editing efficiency and specificity.

The invention relates to a method for culturing cotton resisting a micro-bundle disease. The method comprises the following steps: using sea island cotton or land-sea hybrid cotton with high resistance and immunity to the greensickness and the blight of a main cotton micro-bundle disease as a parental stock; using a general upland cotton specie or strain as scion; grafting at a seedling stage; andculturing grafted seeds in an environment with the temperature of 25-35 DEG C, the relative humidity of 85-95 DEG C and indirect lighting for 5-10 days. The method selects the sea island cotton or the land-sea hybrid cotton as the parental stock and inhibits or prevents micro-bundle pathogens from expanding under the effect of physical obstacle (texture structure) or chemical inhibition thereof or interaction the physical obstacle (texture structure) or the chemical inhibition thereof so as to present immunity or high resistance to the greensickness and the blight of a main cotton micro-bundle disease. The method has high grafting survival rate (higher than 90 percent), grafting cotton has strong advantages, high yield, longer stem than non-graft scion, larger fruit branch number than thenon-graft scion and strong-growth characteristic similar to the parental stock.

The invention discloses a technique for controlling a quick propagation light source for upland cotton, which belongs to the technical field of biology. The technique comprises the following steps of: flushing upland cotton seeds by using running water, sterilizing the flushed seeds in a clean bench, and soaking the sterilized seeds in sterile water for 12 to 24 hours; peeling off seed husks by using forceps, inoculating seed kernels to an MS culture medium, culturing the seed kernels in dark for 2 days, then culturing the seed kernels under fluorescence lamp light for 5 days, and taking apical buds serving as explants after the seed leaves are flattened; cutting about 2 centimeters of apical buds with partial hypocotyls, culturing the cut apical buds under a fluorescence lamp for 5 days, and then culturing the cut apical buds for 40 days under different light sources of fluorescence lamps (contrast) in a ratio of monochrome blue light to red light and blue light of 3 to 1, a ratio of red light to blue light of 1 to 1 and a ratio of red light to blue light of 1 to 3. The method has the advantages of simple operation, quick growth and robustness of plants, energy conservation, environmental protection and the like, and has high practical application value.

The present invention relates to a new cotton (Gossypium hirsutum) variety, and more particularly to cotton plants and cotton seeds of this new variety. The invention also relates to F1 hybrid cotton plants and seeds produced using the new variety, as well as to transgenic cotton plants and seeds produced by transformation of this new variety.