SSR markers lined with major gene of cotton fiber strength

Abstract

The invention discloser SSR markers lined with major genes of cotton fiber strength, which is obtained by the following steps: generating F2 and F2:3 populations by using a cotton search 41 line sGK9708 selected from cultivated varieties of gossypium hirsutum and a high quality line 0-153 of gossypium hirsutum as parents; allowing generation within the family of the F2:3 population to self cross till the F2:6 generation, performing within-family individual selection of the F2:6 generation once, and planting two generation till F6:8; performing polymorphism screening of the parents by using SSR primers and creating an RIL population linkage map; and performing the multi-environment major QTL screening of the cotton fiber strength to screen 6 QTLs of a cotton fiber strength character from line 0-153, wherein 5 QTLs are multi-environment stable QTLs and are FS1 linkage marker NAU2119330, FS2 linkage markers BNL2572125, BNL1064110 and DPL0874210, FS4 linkage markers are NAU1048250 and NAU2627350, and FS5 linkage markers BNL1421200 and NAU2730450. The SSR markers lined with the major genes of the cotton fiber strength are screen from high fiber quality materials and used as molecular markers to perform early auxiliary selection on a DNA level to improve the selection efficiency of the cotton fiber strength.

Description

technical field [0001] The present invention relates to SSR (Simple Sequence Repeats, simple sequence repeat) molecular markers linked from germplasm material 0-153 with excellent fiber quality and main effect gene of cotton high-strength fiber. Combined with the analysis of marker genetic maps, the use of these molecular markers closely linked to fiber strength for assisted selection can improve the efficiency of cotton fiber quality breeding, which belongs to the field of biotechnology applications. Background technique [0002] Cotton fiber is the most important economic trait of cotton, and it is also an important raw material for textile industry. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Before the 1980s, Chinese cotton breeders mainly focused on the improvement of yield, but did not pay enough attention to the imp...

AI Technical Summary

Problems solved by technology

However, most of the previous studies used isolated populations, or the results obtained only in a single environment, so they lacked reliability and stability, and the original purpose of some studies was only for the positioning of target genes. The selection of materials does not consider the combination with breeding materials, and it is difficult to apply them to breeding

Images