SSR markers lined with major gene of cotton fiber strength

A technology of cotton fiber and main effect genes, applied in the direction of microbial determination/inspection, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of difficult breeding, lack of reliability and stability, and no consideration of breeding materials, etc. To achieve the effect of improving the selection efficiency

Inactive Publication Date: 2009-12-30
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, most of the previous studies used isolated populations, or the results obtained only in a single environment, so they lacked reliability and stability, and the original purpose of s

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  • SSR markers lined with major gene of cotton fiber strength

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Embodiment Construction

[0019] The present invention will be further clarified below by the detailed description of specific embodiments, and the molecular marker linked with the main effect gene of cotton high-strength fiber is obtained by the following method:

[0020] (1) Using sGK9708, an upland cotton variety selected by China Cotton Institute 41, bred by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences as the female parent, and the high-strength fiber germplasm line 0-153 with Asian cotton introgression selected by Sichuan Agricultural University as the male parent, In 2001, the hybrid combination was configured in the experimental field of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences, and in 2002, F 1 , selfing produces F 2 . In 2003, F 2 , planting parents and F 1 Two rows for each, 250 individual plants were harvested, and all the individual plants were selfed to obtain F 2:3 seed. The flowers are harvested from individual pl...

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Abstract

The invention discloser SSR markers lined with major genes of cotton fiber strength, which is obtained by the following steps: generating F2 and F2:3 populations by using a cotton search 41 line sGK9708 selected from cultivated varieties of gossypium hirsutum and a high quality line 0-153 of gossypium hirsutum as parents; allowing generation within the family of the F2:3 population to self cross till the F2:6 generation, performing within-family individual selection of the F2:6 generation once, and planting two generation till F6:8; performing polymorphism screening of the parents by using SSR primers and creating an RIL population linkage map; and performing the multi-environment major QTL screening of the cotton fiber strength to screen 6 QTLs of a cotton fiber strength character from line 0-153, wherein 5 QTLs are multi-environment stable QTLs and are FS1 linkage marker NAU2119330, FS2 linkage markers BNL2572125, BNL1064110 and DPL0874210, FS4 linkage markers are NAU1048250 and NAU2627350, and FS5 linkage markers BNL1421200 and NAU2730450. The SSR markers lined with the major genes of the cotton fiber strength are screen from high fiber quality materials and used as molecular markers to perform early auxiliary selection on a DNA level to improve the selection efficiency of the cotton fiber strength.

Description

technical field [0001] The present invention relates to SSR (Simple Sequence Repeats, simple sequence repeat) molecular markers linked from germplasm material 0-153 with excellent fiber quality and main effect gene of cotton high-strength fiber. Combined with the analysis of marker genetic maps, the use of these molecular markers closely linked to fiber strength for assisted selection can improve the efficiency of cotton fiber quality breeding, which belongs to the field of biotechnology applications. Background technique [0002] Cotton fiber is the most important economic trait of cotton, and it is also an important raw material for textile industry. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Before the 1980s, Chinese cotton breeders mainly focused on the improvement of yield, but did not pay enough attention to the imp...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12P19/34
Inventor 袁有禄孙福鼎石玉真李俊文刘爱英张建宏王淑芳龚举武巩万奎王涛商海红
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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