523 results about "Sequence repeat" patented technology

The invention provides a method for developing a mung bean simple sequence repeat (SSR) primer based on transcriptome sequencing. The method comprises the following steps: obtaining a set of mung bean genome-wide transcription, and forming a sequence database; splicing sequencing sequences into a transcriptome by Trinity; taking the longest transcript in each gene as Unigene; carrying out bioinformatics analysis of a Unigene sequence; carrying out SSR detection on the Unigene by adopting MISA1.0; carrying out SSR primer design by using a Primer 3, and carrying out SSR primer polymorphism identification. 13134 pairs of SSR primers are successfully designed by application of the method; 50 pairs of primers are randomly selected to verify 8 parts of mung bean deoxyribonucleic acids (DNAs) from different countries, wherein 32 pairs of polymorphic primers are formed in all; the mung bean materials with different geographical origins can be distinguished by using the 32 pairs of SSR primers. The method disclosed by the invention is convenient, fast and accurate, and low in cost, and a new thought is provided for development of the mung bean SSR primer.

A method for obtaining an intraoral x-ray image determines an initial spatial position and angular orientation of an x-ray source relative to a detector. A first x-ray image is obtained with the x-ray source at the initial spatial position and angular orientation and stored, associating the initial spatial position and angular orientation to the first x-ray image. A sequence repeats that calculates a next spatial position and angular orientation for the x-ray source, provides positional adjustment information between the x-ray source and detector for obtaining a next x-ray image, measures and records the actual spatial position and angular orientation of the x-ray source relative to the detector and obtains and stores the next x-ray image at the measured spatial position and angular orientation, and forms a composite image using image data from the first and from the one or more next x-ray images.

A method of generating a code sequence and method of adding additional information using the same are disclosed, by which a code sequence usable for a channel for synchronization is generated and by which a synchronization channel is established using the generated sequence. The present invention, in which the additional information is added to a cell common sequence for time synchronization and frequency synchronization, includes the steps of generating the sequence repeated in time domain as many as a specific count, masking the sequence using a code corresponding to the additional information to be added, and transmitting a signal including the masked sequence to a receiving end.

The invention discloses a method for massively and efficiently developing molecular markers on the basis of Indel and SSR (simple sequence repeat) site techniques. The method comprises the following steps: (1) selecting at least 3 samples to be developed, and respectively extracting DNAs (deoxyribonucleic acids) of the samples to be developed; (2) carrying out enzyme digestion on the DNA samples of the samples to be developed, establishing a sequencing library, and carrying out sequencing; (3) mixing the genomes of all the samples to be developed, and assembling to obtain Contigs; (4) comparing the Contigs with the sequences of the sample individuals to be developed, and acquiring SSR sites with Indel inside according to the Indel and SSR site information as a candidate polymorphism SSR site; (5) designing primers according to the obtained candidate polymorphism SSR site, carrying out PCR (polymerase chain reaction) amplification and sequencing, and selecting a ribbon-shaped stable clear strip as molecular marker primers to be verified; and (6) carrying out PCR amplification on different samples to be developed by using the obtained molecular marker primers, and selecting the molecular markers with diversity, thereby obtaining the molecular markers. The method enhances the molecular marker development efficiency; and the developed SSR molecular markers can be efficiently applied to research in the aspects of genetics, multiplication release evaluation and the like.

The invention discloses an oil-tea SSR molecular marker which comprises acquisition and pre-treatment of an oil-tea DNA sequence, retrieval of SSR loci in the DNA sequence, design and synthesis of an SSR primer, establishment of an SSR marker system, and screening of a polymorphic SSR marker. Oil-tea genomic sequences obtained by 454 sequencing and eligible sequences containing simple sequence repeat units in an EST sequence are retrieved and screened to obtain twelve polymorphic SSR markers: CO_gSSR11, CO_gSSR12, CO_gSSR14, CO_gSSR16, CO_gSSR19, CO_gSSR20, CO_eSSR01, CO_eSSR04, CO_eSSR16, CO_eSSR40, CO_eSSR44 and CO_eSSR48. The oil-tea SSR molecular marker disclosed by the invention is suitable for genetic evaluation, germplasm identification and molecular marker assisted breeding research of oil-tea germplasm resources, has the advantages of good repeatability and high reliability, and is an effective molecular marker.

The present invention relates to set of inter-simple sequence repeats (ISSR)-PCR primers of SEQ ID Nos. 1 to 37 for genotyping eukaryotes and a method of genotyping diverse genomes of plant and animal systems using FISSR-PCR primers and SSR markers; more particularly, a FISSR and SSR method of distinguishing Basmati rice varieties from Non-Basmati (NB) rice varieties, and Traditional Basmati (TB) rice varieties from Evolved Basmati (NB) rice varieties, and also, a method for determining adulteration of Basmati rice with other rice varieties and a kit thereof.

The invention relates to a method for identifying the purity of a tobacco variety Zhongyan 90 by using a specific molecular marker method, wherein the purity of the flue-cured tobacco variety Zhongyan 90 is identified by using an SSR (Simple Sequence Repeats) molecular marker. According to the invention, based on a large amount of experiments, a specific primers of a characteristic strip of Zhongyan 90 are obtained through amplification, the specific primer sof Zhongyan 90 are used to carry out purity detection, detection results are reliable and visual, and compared with the conventional detection method, the method disclosed by the invention is simple to operate, high in detection efficiency and lower in cost. With adoption of the method, the detection period is short, that is, the purity of a seed can be detected in a seedling stage by using the method, in addition, standards are unified and are not influenced by human factors and environment, and results are accurate and reliable.

The invention belongs to biotechnology field, and relates to a method for developing a dendranthema SSR (Simple Sequence Repeat) primer based on transcriptome sequencing. A lot of sequence information is processed in batch for searching an SSR sequence and designing an SSR labeled primer based on the transcriptome sequencing by utilizing EST-SSR (Expressed Sequence Tags-Simple Sequence Repeat) interspecific metastasis and using a Perl (Practical Extraction and Reporting Language) programming language method in combination, so that the defects of low efficiency, long time consumption, high cost and the like of the SSR development are conquered. Different dendranthema materials are selected for verifying the designed SSR primer, and the primer is a successful SSR primer if detected out by any strip. By adopting the method, 1788 pairs of SSR primers are successively designed, so that a novel method and thinking are provided for the development of the dendranthema SSR primer to further achieve molecular marker assistant selection breeding and comparative genomics research.

The invention provides a lysinecorn SSR (simple sequence repeat) molecular marker auxiliary selecting and breeding method. By combining a backcross transforming method with a molecular marking method, the method successfully constructs the lysinecorn isogenic gene systems QZ58, Q478 and QC72. After the SSR molecular marker auxiliary selecting and breeding method is used the mono-clones which contain the O2 gene can be detected by SSR molecular marker in the O2 gene after one generation is backcrossed, and the second generation can be backcrossed without selfing, so that the breeding is short in period, low in cost, and high in efficiency. Aiming at different breeding target requirements, the method is used for performing the gene type detection to the backcross offspring seedling leaves; and the method is not influenced by the environment conditions and combines with south propagating and generation adding, so that the target genes are transferred into a good material within a shorter time.

The invention discloses an inter-simple sequence repeat ISSR-SCAR marker specific to E-group chromosomes of agropyron elongatum. The invention provides a kit for detecting the E-group chromosomes of agropyron elongatum. The kit comprises the following primer pair: one primer in the primer pair is nucleotide shown in a sequence 3 of a sequence table, and the other primer in the primer pair is nucleotide shown in a sequence 4 of the sequence table. The E-group chromosomes of thinopyrum elongatum are the basic chromosome group forming the polyploid species of elytrigia and carry the genes beneficial to the genetic breeding of wheat, the detection of exogenous E chromatins in wheat is the key point for identifying recombination lines and introgression lines of the wheat, namely the agropyron elongatum, the work of screening and identifying a large number of filial generations is quite complicated, and tests prove that the ISSR-SCAR marker developed by the invention can lay a foundation for quickly and accurately identifying the E-group chromatins or chromosomes of exogenous agropyron elongatum under the genetic background of wheat.

The present invention discloses the seedless litchi graft stock selecting technology. The technological process of selecting seedless litchi graft stock includes the following steps: 1. separating the simple sequence repeating (SSR) sequence through a combined selective amplifying microsatellite (SAM) and database searching method; 2. designing SSR primer with the flanking sequence of SSR sequence; 3. screening out the useful primer to obtain litchi specificity SSR marker; 4. applying the litchi specificity SSR marker in the genetic diversity analysis of partial litchi germplasm; 5. data analysis; and 6. selecting the litchi germplasm with near affinity as stock for seedless litchi grafting propagation based on the analysis result. The said process results in high survival rate and can obtain high quality seedless litchi variety.

The invention discloses a method for inspecting purity of two-line hybrid rice seeds by utilizing an SSR (Simple Sequence Repeat) method, in particular a method for inspecting the purity of Fengliangyou No. 1 rice or Fengliangyouxiang No. 1 rice seeds by utilizing SSR molecular markers. The method comprises the following steps of: performing amplification and agarose electrophoretic separation on genome DNA (Deoxyribonucleic Acid) of the two-line hybrid rice varieties Fengliangyou No. 1 and parents thereof or Fengliangyouxiang No. 1 and parents thereof respectively by utilizing primers P1 and P2 to find out a characteristic spectrum band of difference between a first-filial generation and the parents; inspecting the samples of Fengliangyou No. 1 or the Fengliangyouxiang No. 1 by calculating the purity of the inspected seeds; and comparing and contrasting the inspected purity with purity tested by field planting to confirm that the results of the two are highly consistent. The method is suitable for inspecting the authenticity and variety purity of the Fengliangyou No. 1 or Fengliangyouxiang No. 1 two-line hybrid rice and parents thereof.

There are disclosed a method of communicating for a smart utility network using a TV white space and an apparatus for the same. The method of communicating for a smart utility network using a TV white space according to the present invention includes: generating a time domain sequence repeated every predetermined number of samples; generating an OFDM symbol having a cyclic prefix length corresponding to an FFT size divided by a natural number of 2 or more and including samples of a number corresponding to the sum of the FFT size and the cyclic prefix length; and generating an SUN packet to be transmitted through a TV channel band selected in the TV white space by using the time domain sequence and the OFDM symbol. Accordingly, it is possible to satisfy all requirements required by the IEEE 802.15.4g SUN standardization group.

The invention discloses a method for breeding high-yield high-oil peanut species. In the method, a genetic relationship of the selected high-yield high-oil peanut species is found out by an SSR (Simple Sequence Repeat) molecule marker technology; the species with distant hybrid advantages and larger selection difference is used as a parent for preparing cross combination; the variation frequency is expanded by radiation effect, and the selecting range of a high-yield high-oil gene is expanded; the genetic linkage of an adverse gene is broken down by applying hybridization, and the recombination, polymerization and complementation of high yield, high oil and resistance are realized; an individual plant is directionally selected by using ideal characters as a target in stable generation; and the individual plant is inbred for one generation, a plant system with complete and ideal characters is further selected, and the high-yield high-oil plant system is obtained. The invention has the characteristics of rapidly breeding the high-yield high-oil peanut new species and having simple method and high operability.

The invention belongs to the field of forage grass germplasm resource identification, in particular to a method for identifying pennisetum forage grass by utilizing an ISSR (Inter Simple Sequence Repeat) molecular marker technology, wherein primers used by ISSR-PCR (Polymerase Chain Reaction) are UBC815 and UBC835, and the nucleotide sequences of the primers are respectively UBC815-CTCTCTCTCTCTCTCTG and UBC835-AGAGAGAGAGAGAGYC. The method fills the blank of distinguishing pennisetum forage grass categories (varieties) by applying the ISSR molecular marker method, and an electrophoresis ideograph finally drawn by the method for distinguishing seven pennisetum forage grass materials for tests can be used as a basis for distinguishing and identifying the seven materials. The method gets rid of unreliable factors of traditional morphology identification and can quickly and accurately distinguish the seven pennisetum forage grass materials, and an identification result can be used as a reliable basis for approving categories, varieties and strains so as to guarantee the great popularization of germplasm on production.

The invention discloses a method for identifying a commercial cigarette by applying an SSR (Simple Sequence Repeat) molecular marker technique, belonging to the field of molecular biology. The method comprises the steps of carrying out genetic map analysis on a tobacco in single variety by applying the SSR technique, screening out SSR sites with moderate aberration rate and variety specificity, extracting total DNA (deoxyribonucleic acid) of commercial cigarette shreds, amplifying by using screened specific primers, comparing genetic variation type with the type of the single variety, distinguishing the variety of tobacco leaves contained in the commercial cigarette shreds according to the variety specificity sites, and contrasting the using condition of the variety of the tobacco leaves in the corresponding cigarette formula to conveniently identify the authenticity of the cigarette. Meanwhile, the method is also suitable for the identification of tobacco leaves in single variety, tobacco shred groups and tobacco shred samples in mixed varieties.

The invention discloses a method for identifying authenticity of sweet potato variety by using an inter-simple sequence repeat (ISSR) molecular marker method. A disclosed primer group used for identifying the authenticity of the sweet potato variety in an assisted mode consists of a primer HBAAS-001, a primer HBAAS-815 and a primer HBAAS-825, wherein the nucleotide sequence of the primer HBAAS-001 is shown as SEQ ID No:1, the nucleotide sequence of the primer HBAAS-815 is shown as SEQ ID No:2, and the nucleotide sequence of the primer HBAAS-825 is shown as SEQ ID No:3. An ISSR fingerprint spectrum of a single primer of the sweet potato variety is successfully constructed according to an ISSR molecular marker, so that the method for identifying the authenticity of the sweet potato variety is simple, convenient, rapid, high in repeatability and high in stability, and a solid foundation is laid for allowing an ISSR molecular marker technology to be widely applied to identifying the authenticity of the sweet potato variety.

The invention belongs to the field of genetic breeding science and discloses a molecular marking method for a major quantitative trait loci (QTL) for rice grain length. In the method, Indel and simple sequence repeats (SSR) are used to mark one or more pairs of primers of XJ24 and XJ26 and RM15575 respectively to perform the polymerase chain reaction (PCR) amplification of rice genome DNA, PCR amplification products undergo an electrophoretic test on 8 percent non-denaturing polyacrylamide gel, and if DNA fragments in corresponding size are generated through amplification, qGL 3.2 synergisticallelic mutant genes exist. When the large-grain germplasm of rice and breeding populations constructed by various parents of rice are detected by the grain length QTL qGL3.2 coseparated and closely interlocked molecular marking method, the major gene qGL 3.2 for controlling grain length and weight can be quickly imported, the selecting efficiency of the major gene qGL 3.2 is improved, and the method contributes to appearance improvement and high-yield breeding of rice.

The invention discloses an ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and an identification method of the marker. The method includes: performing total DNA (deoxyribose nucleic acid) extraction, PCR (polymerase chain reaction) amplification, primer selection and ISSR-SCAR detection on Wujiang brassica chinensis and other 10 brassica chinensis varieties, and finally determining an ISSR-SCAR molecular marker and a molecular identification method of the Wujiang brassica chinensis. A primer shown in the sequence such as SEQ ID NO:1 is used for amplification to obtain a specific fragment shown in the sequence such as SEQ ID NO:2. According to the sequence, a pair of primers in the sequences such as SEQ ID NO:3 and SEQ ID NO:4 is designed. The marker is simple to operate, stable and reliable, is the first molecular identification marker applied to the variety of Wujiang brassica chinensis, can be used for quickly identifying the variety and has significance of protecting germplasm resources of geographical indication products.

The invention discloses an SSR (simples sequence repeats) and InDel (insertion/deletion) molecular marker primer linked with brassica campestris orange head gene Br-or and an application of the molecular marker primer. The marker primer is obtained by the steps of: based on the genome DNA (deoxyribonucleic acid) of orange brassica campestris and normal white brassica campestris and F2S4 separation group as a template, carrying out polymorphism primer screening to orange head and white head single strain DNA mixed tank in F2S4 by using 480 pairs of SSR and InDel primer pairs, then analyzing single strain of F2S4 group, and screening the molecular marker linked with the brassica campestris orange head gene Br-or to finally obtain fifteen Br-SSR and three Br-InDel molecular markers linked with the Br-or gene, wherein a molecular genetic map of the brassica campestris orange head gene Br-or is created on the ninth link group (A09). According to the link analysis, the genetic distance of two markers most tightly linked with both sides of the Br-or gene are 0.11cM and 0.79cM; and the molecular marker primer has the advantages of being convenient for detection, stable in amplification, and high in repeatability and accuracy, and thus a basis is provided to the molecule assistant selective breeding of brassica campestris orange head character and the breeding process is accelerated.

The invention relates to a high sensitive and jettisonable multicomponent chemiluminescent imaging immunosensor. The immunosensor is prepared by: constructing a 4*12 array on a silanized glass slide by a screen printing technique, and coating the array points with different capture antibodies to construct a jettisonable multicomponent immune sensor array; immobilizing biotinylated capture DNA and multiple G-quadruplex sequence repeat signal DNA on gold nanoparticle surfaces simultaneously, combining G-quadruplex signal DNA with heme to form DNA enzyme so as to prepare a multilayer DNA enzyme functionalized gold nanoparticle probe; and based on sandwich immunoassay, forming a sandwich immune complex on the sensor array, carrying out biotin-avidin reaction, labeling different immune complexes with the multilayer DNA enzyme functionalized gold nanoparticle probe; and making use of the peroxidase characteristics of DNA enzyme to catalyze reaction between chemiluminescent substrate H2O2-luminol to obtain a sensitive chemiluminescent signal, thus realizing high sensitive image immunoassay of a variety of protein. The immunosensor has the advantages of simple design, low cost, high sensitivity, high throughput and good repeatability, etc., and has certain clinical application value.