Seedless litchi graft stock selecting technology

Abstract

The present invention discloses the seedless litchi graft stock selecting technology. The technological process of selecting seedless litchi graft stock includes the following steps: 1. separating the simple sequence repeating (SSR) sequence through a combined selective amplifying microsatellite (SAM) and database searching method; 2. designing SSR primer with the flanking sequence of SSR sequence; 3. screening out the useful primer to obtain litchi specificity SSR marker; 4. applying the litchi specificity SSR marker in the genetic diversity analysis of partial litchi germplasm; 5. data analysis; and 6. selecting the litchi germplasm with near affinity as stock for seedless litchi grafting propagation based on the analysis result. The said process results in high survival rate and can obtain high quality seedless litchi variety.

Description

technical field [0001] The present invention relates to the technical field of fruit tree grafting, in particular to a technical method for solving the problem of seedless litchi grafting incompatibility by applying Simple Sequence Repeat (SSR) molecular markers Background technique [0002] Litchi (Litchi chinensis Sonn.) is a plant of the genus Litchi in the family Sapindaceae. It is well-known at home and abroad for its brightly colored fruit, tender and juicy flesh, sweet and delicious taste, and has "fairy fruit on earth", "Buddha fruit", The reputation of "King of Fruits". Lychee pulp has high nutritional value. Every 100 grams of fruit juice contains 36 mg of vitamin C, 0.7 grams of protein, 0.6 grams of fat, and 14 grams of carbohydrates, generating 267.7760 kilojoules of heat. Traditional Chinese medicine believes that lychee is sour in taste and warm in nature. Dry embolism has miraculous health care effect. Therefore, folks often use litchi as a medicinal tonic...

AI Technical Summary

Problems solved by technology

The main symptoms are ① swelling of the graft interface, which is obviously large and small, and the healing is abnormal; ② the healing of the graft interface is basically normal, and there are 1 or 2 times of normal shoots, but after that, only short shoots cluster at the tips, and even short shoots. Small flower spikes, unable to produce fruit; ③ After planting, the seedlings turn yellow, unable to pull out shoots, and maintain a long-term condition of growth; ④ The original growth is normal and has been fruiting for many years. As time goes by, it gradually shows that the growth of the grafting interface is uneven. Yellowing and decline of the aboveground part

Plants that are not compatible for grafting should be identified at an early stage. However, the traditional rootstock and ear matching test period is long and the cost is high, so it is difficult to solve this problem quickly and effectively.