Seedless litchi graft stock selecting technology

A new technology, the technology of nuclear litchi, is applied in the field of non-nuclear litchi grafting incompatibility, which can solve the problems of seedling yellowing, abnormal healing, and long test period.

Inactive Publication Date: 2007-01-03
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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Problems solved by technology

The main symptoms are ① swelling of the graft interface, which is obviously large and small, and the healing is abnormal; ② the healing of the graft interface is basically normal, and there are 1 or 2 times of normal shoots, but after that, only short shoots cluster at the tips, and even short shoots. Small flower spikes, unable to produce fruit; ③ After planting, the seedlings turn yellow, unable to pull out shoots, and maintain a long-term condition o

Method used

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Examples

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Embodiment Construction

[0019] The present invention will be further understood from the following examples:

[0020] 1. Source of materials: 32 Hainan-specific lychee varieties such as seedless lychee, A13, and seedless clove, a few Guangdong lychee varieties such as Baitangying, Feizixiao, and Heiye, and two main varieties of longan—Shi Xia and Chuliang were used as experimental materials.

[0021] 2. Method:

[0022] (1), Separation of SSR sequence:

[0023] 1) Use seedless lychee as material, use Selectively Amplified Microsatellite (SAM) method to separate SAM fragments, select 100 SAM fragments to recover, clone, and sequence, and then use Sputnik software to analyze the sequencing results to obtain SSR sequence.

[0024] Result analysis: almost all fragments contain SSR sequence. Among them, most of the sequenced fragments contain dinucleotide CT / TC, that is, AG / GA repeats (this is consistent with the SSR primer—PCT6 used in SAM amplification), and the number of repeats is mostly 6, such f...

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Abstract

The present invention discloses the seedless litchi graft stock selecting technology. The technological process of selecting seedless litchi graft stock includes the following steps: 1. separating the simple sequence repeating (SSR) sequence through a combined selective amplifying microsatellite (SAM) and database searching method; 2. designing SSR primer with the flanking sequence of SSR sequence; 3. screening out the useful primer to obtain litchi specificity SSR marker; 4. applying the litchi specificity SSR marker in the genetic diversity analysis of partial litchi germplasm; 5. data analysis; and 6. selecting the litchi germplasm with near affinity as stock for seedless litchi grafting propagation based on the analysis result. The said process results in high survival rate and can obtain high quality seedless litchi variety.

Description

technical field [0001] The present invention relates to the technical field of fruit tree grafting, in particular to a technical method for solving the problem of seedless litchi grafting incompatibility by applying Simple Sequence Repeat (SSR) molecular markers Background technique [0002] Litchi (Litchi chinensis Sonn.) is a plant of the genus Litchi in the family Sapindaceae. It is well-known at home and abroad for its brightly colored fruit, tender and juicy flesh, sweet and delicious taste, and has "fairy fruit on earth", "Buddha fruit", The reputation of "King of Fruits". Lychee pulp has high nutritional value. Every 100 grams of fruit juice contains 36 mg of vitamin C, 0.7 grams of protein, 0.6 grams of fat, and 14 grams of carbohydrates, generating 267.7760 kilojoules of heat. Traditional Chinese medicine believes that lychee is sour in taste and warm in nature. Dry embolism has miraculous health care effect. Therefore, folks often use litchi as a medicinal tonic...

Claims

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Application Information

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IPC IPC(8): A01G1/06
Inventor 李明芳郑学勤吴新荣王高罗振标洪钢池
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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