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Method for cultivating high-affinity fusarium wilt-resistant tomato rootstock

A technology of resistance to fusarium wilt and affinity, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as weak root stock, no harvest, and reduced tomato yield.

Pending Publication Date: 2019-01-11
QINGDAO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for cultivating high-affinity fusarium wilt-resistant tomato rootstocks, which can effectively solve the problem that the rootstocks bred by the existing fusarium wilt-resistant rootstock breeding methods have weak root systems, and the rootstocks have delayed grafting incompatibility characteristics, causing tomato yield reduction or even failure, and it is difficult to apply in a large area in production

Method used

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  • Method for cultivating high-affinity fusarium wilt-resistant tomato rootstock
  • Method for cultivating high-affinity fusarium wilt-resistant tomato rootstock

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Effect test

Embodiment 1

[0017] The breeding of embodiment 1 resistance to fusarium wilt and resistance to root-knot nematode tomato stock

[0018] From 2008 to 2014, screening of disease-resistant rootstock materials was carried out for many years in a field disease nursery where tomato had been planted continuously for more than 10 years and the incidence rate of Fusarium wilt and root-knot nematode was 100%. Among them, the root-knot nematode-resistant strains 'Z120-5-3', 'Z120-5-5', and 'Z123-2' showed vigorous plants, dark green leaves, well-developed roots, and root nematode index was 0%; resistance to wilting The diseased lines 'Z05-1-4' and 'Z05-1-5' showed vigorous plants, dark green leaves, well-developed roots, root nematode index was 6%, and the incidence of Fusarium wilt was 0; the control 'Z121' showed The leaves were yellow, the plants were weak, and the root nematode index was 61.2%. Among them, 'Z05-1-5' was the most prominent among the 12 sown lines. The plants grew vigorously, with...

Embodiment 2

[0027] The identification of embodiment 2 stock grafting affinity

[0028] Previous studies have found that when '72-69' is used as a scion to graft different rootstocks, the nutrient absorption rate of the root system of the rootstock will be slower than the development rate of the scion fruit, resulting in poor compatibility. It shows that the rootstocks with good grafting compatibility will grow normally after grafting; while the rootstocks with poor grafting compatibility will grow vigorously at the seedling stage and early fruit expansion stage after grafting, but the plants will wilt or even die in the late fruit expansion stage. Phenomenon.

[0029] 2.1 Materials and methods

[0030] In the spring of 2012, grafting compatibility tests were carried out on 48 rootstock hybrid combinations using '72-69' as scion. The experiment was arranged in Qingdao Academy of Agricultural Sciences. On February 4, tomato rootstock and scion varieties were sown in seedling trays. On Ma...

Embodiment 3

[0038] Embodiment 3 verification test

[0039] Identification of Grafting Compatibility of Different Types of Scions to Rootstock ‘Z 05-1-5×Z 123-2’

[0040] 3.1 Materials and methods

[0041]In the spring and autumn of 2013, the rootstock 'Z 05-1-5×Z 123-2' was grafted with 'Qingyan Tomato No. 1' and 'Fensha No. 2' as scions respectively in the steel pipe shed of Qingdao Academy of Agricultural Sciences Product comparison test. 'Qingyan Tomato No. 1' matures early, and the fruit expands quickly. It is used as a scion. It has strict requirements on rootstocks, and rootstocks with strong compatibility can be selected. The area of ​​the cultivated plot is 3.6 square meters, the distance between plants is 0.3 meters, and the distance between rows is 0.6 meters. 20 trees are planted in each border, and repeated three times.

[0042] 3.2 Test results

[0043] 3.2.1 'Qingyan Tomato No.1' as scion

[0044] In the spring of 2013, 'Qingyan Tomato No.1' was used as the scion, and '...

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Abstract

The invention provides a method for cultivating a high-affinity fusarium wilt-resistant tomato rootstock, and belongs to the field of tomato grafting rootstock breeding. The problems of yield reduction or even no harvest and difficult large-area application in production of tomatoes, caused by weak roots, slow growth and grating non-affinity of rootstock bred by existing fusarium wilt-resistant rootstock breeding methods are solved in the invention. The method comprises the following steps: selecting a specific field disease nursery, planting tomato rootstocks, evaluating the characteristics of the tomato rootstocks by introducing two indexes of root development degree and fusarium wilt-resistant incidence, selecting a rootstock inbred line having developed roots and having resistance to fusarium wilt, and carrying out field affinity identification on the bred rootstock inbred line in the whole growth period by identifying scions in order to obtain the high-affinity fusarium wilt-resistant tomato rootstock. The method can be applied to the production of various varieties of tomatoes.

Description

technical field [0001] The invention relates to a method for breeding tomato grafted rootstocks, in particular to a method for cultivating tomato rootstocks with high affinity and resistance to Fusarium wilt. Background technique [0002] Tomato is one of the most important vegetable crops in the world and occupies an important position in vegetable cultivation in my country. Tomato wilt is an important soil-borne disease affecting tomato production. Tomato wilt is a vascular bundle caused by Fusarium oxysporum f.sp lycopersici (sacc) snyder et Hansen. Disease, the fungus mainly infects plant vessel tissue, causing plant wilting by blocking xylem matter and water transport. Tomato Fusarium wilt begins at the flowering and fruiting stage. At the beginning of the disease, only one side of the stem appears a depression from bottom to top, causing one side of the leaf to turn yellow, turn brown and then die. The diseased stem is cut open, and the vascular bundle turns brown. G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01G2/30
CPCA01G2/30A01H1/04
Inventor 黄婷婷李平刘淑芹张永志张功臣佘红
Owner QINGDAO ACAD OF AGRI SCI
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