229 results about "Vascular bundle" patented technology

The invention relates to a composite material for manufacturing an automotive interior trim part. The bamboo fiber composite base material for manufacturing the automotive interior trim part is prepared by taking thermoplastic synthetic fibers (polypropylene PP, polyethylene PE or polyester PET and the like) as a bonding substrate and bamboo fibers as a reinforcing material through hot pressing. The bamboo fiber composite base material is characterized in that the bamboo fibers are prepared by sawing natural bamboo into pieces with length required by production, removing parenchyma from the bamboo by mechanical, physical, chemical and biological methods and directly extracting vascular bundle bamboo fibers from the bamboo, and have the length of 60 to 150mm, the diameter of 0.3 to 1.5mm, the strength of 60 to 120MPa and the water content of 10 to 12 percent. The automotive interior trim part can be subjected to press forming when the bamboo fiber composite base material covers artificial leather, leather or a knitted or non-woven fabric for surface decoration.

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

The invention discloses a dragon fruit grafting method, tender shoots of dragon fruit is taken as scion, and a wedge grafting method is used for grafting; and the method comprises the steps of: a, taking the scion and obtaining the tender shoots of the dragon fruit by using a pair of pruning scissors or a grafting cutter; and b, grafting: firstly cutting the top end of a tree stock transversely, cutting with a seam longitudinally at the transversely-cut center of top end of the tree stock, then chopping the lower part of the scion into wedge shaped, and inserting the scion into the seam of the tree stock immediately after chopping so that the tree stock can be matched with vascular bundle of the scion. The dragon fruit grafting method has the characteristics of high grafting survival rate, high shoots drawing speed and the like, and the survival rate of the grafted dragon fruit survival rate can reach above 95%.

The invention relates to an induction-enhanced tissue specific promoter and application thereof, belonging to the technical field of plant genetic engineering. A promoter (BPHP, i.e. Bph14 promoter) for Oryza Sativa L. genes resistant to brown plant hoppers is separated from Oryza Sativa L. and is cloned. The promoter has a nucleotide sequence shown as SEQ ID No. 1, can control Oryza Sativa L. genes resistant to the brown plant hoppers to be specifically expressed in vascular bundle tissues and can be expressed in a way that the feeding induction of the brown plant hoppers is enhanced. The promoter contains BS1EGCCR cis-acting elements and can enable the genes to be specifically expressed in plant vascular bundle tissues. Moreover, the promoter has defense-related response cis-acting elements which can be combined with WRKY transcription factors, and the gene expression is increased when Oryza Sativa L. is fed by the brown plant hoppers or is infected by pathogenic bacteria. The promoter can be used as the promoter for the specific expression in the vascular bundle tissues and can be used as the brown plant hopper and pathogenic bacteria induction-enhanced promoter.

The invention discloses chitin organic fertilizer produced by using livestock excrement and shrimp and crab shells and a preparation method thereof. The chitin organic fertilizer produced by using the livestock excrement and the shrimp and crab shells comprises, by weight percentage, the following raw materials of 65 to 84 % of the livestock excrement, 5 to 15 % of waste of the shrimp and crab shells, 10 to 20 % of straw and 0.5 to 1 % of microbial agent. The chitin organic fertilizer has the advantages that a strong induction effect in the respect of restraining tobacco bacterial wilt is achieved, the tobacco is promoted to generate system resistance under the environmental stress of soil-borne disease, the soil biological activity can be promoted to improve at the same time, the occurrence of the conditions of tobacco vascular bundle deformation and xylem blocking can be reduced to a certain extent, therefore the efficient prevention and control over the tobacco bacterial wilt are achieved, and the problems that the organic fertilizer is slow in taking effect, the effect is not obvious after the fertilizer is applied, and the fertilizer performance is not stable are solved. In addition, the invention further discloses the preparation method of the chitin organic fertilizer.

The invention relates to a cotton continuous cropping high yield cultivation method, aiming at solving the technical problem of overcoming continuous cropping obstacles to achieve high yield cotton. In the invention, sea-island cottons or land-sea hybrid cottons which are highly resistant or immune to vascular bundle disease verticillium wilt and fusarium wilt are taken as rootstocks; conventional upland cottons variety with good affinity to the rootstocks are taken as scions; grafting is carried out during seedling stage; the grafted seedlings are placed in the environment avoiding light direct radiation with the temperature of 25-35 DEG C and the relative humidity of 85-95 percent for cultivating 5-10 days, and then are transplanted to a field with the density of 12000-18000 plants/hm2; and conventional field management is carried out after removing surplus buds on cotyledonary node position. The cotton plants formed through grafting has the advantages of high stress resistance and yield, great growth vigor in the continuous cropping cotton field, and excellent quality; moreover, by selecting the rootstocks and the scions with good affinity during cultivation, the grating survival rate is high, the costs on cultivation and improved varieties are low, and the popularization is easy.

The present invention discloses a specific gene controlling growth of Arabidopsis vascular bundle and application thereof. The gene codes the protein of amino acid sequence shown in SEQ ID No.2 in sequence list and the genome gene is AT5g62940; the corresponding cNDA sequence is shown in the SEQ ID No.1 of the sequence list. As shown from research, the gene codes a transcription factor which is positioned in a core and has trans-activation activity, but the transcription factor which can regulate the formation of the Arabidopsis interfascicular cambium and the growth of vascular tissue is the transcription factor which is found as the first transcription factor for regulating the interfascicular cambium in Arabidopsis. In a transgenic gene plant, overexpression of the gene can thicken the stem of the plant, but inhibiting the expression of the gene in the plant can inhibit the occurrence of the interfascicular cambium. Therefore, the gene can be used for regulating the thickness of the stem, researching the woody plants with growth strength of secondary growth, and improving the quality. The gene has wide application prospect in regulating thickness growth and growth of the wood, flowers and plants.

The invention aims to solve the problems of over spread of large-sized vascular plants in grass-type lakes and over-strong biological deposit-promoting effect and provide a technique and a method for restraining grass and controlling sludge for improving the growing water quality condition of aquatic plants and accelerating bottom sludge decomposition. Iron, aluminum and calcium oxides in cinder can strongly adsorb phosphate radical in the water body, the loose and porous surface of the cinder is favorable for the fixation and growth of soil microbes, and a formed biological membrane can effectively remove organic substances and ammonia nitrogen from the water body and accelerate the biological decomposition of the lake bottom sludge. The cinder is filled in a nylon bag and the nylon bag is fixed in a ground cage for facilitating reclamation and preventing physical deposition at the lake bottom. By using the existing resources in rural areas of China and using the local materials, the method is convenient and feasible, and has strong operability and wide application; and the implementation of the technique does not affect the primary ecological function of the ecosystem of the lake and has significance for maintaining the ecological health of the grass-type lakes for long term.

This invention provides an external use medincet for treating chronic rhinitis and nasosinusitis. It is prepared by using the following Chinese medical ingredients according to their weight ratio: American ginseng 15-3, peony root bark 12-5,Chinese angelica root 12-5,towelgourd vascular bundles 10-5,balloon-flower 10-5,puncturevine caltrap fruit 10-5, coptis rhizome0-5,membranous milkvetch root 10-5,ginseng 10-5,sevenlobed yam rhizome 8-5,pumice8-5,fructus forsythiae 8-5,Dahurian angelica root 5-1,biond magnolia flower-bud 5-1,skebia stem 5-1,siberian cocklebur fruit 5-1,cassiabarktree bark 3-1,manchurian whildginger herb 3-1.In the aspect of the medical application, it is convenient and easy to use with light side effects, no irritatations to the skin and obvious curative effects. According to statistics that the total effective ratio for treating chronic rhinitis, chronic nasosinusitis is above 95%.

The invention discloses a pitaya grafting method which comprises scion processing and grafting. The scion processing means cutting off all the phloem on the lower portion of pitaya scions. The grafting includes the steps that pitaya root stocks are broken, cross sections of the pitaya root stocks are leveled, the parts with the scion phloem cut off are inserted into the phloem of the pitaya root stocks from the cross sections of the pitaya root stocks, the scion inserting parts are tightly attached to the pitaya root stocks to leave no gap. By means of the method, the complex and toilsome processes that cactus thorns or toothpicks are stabbed into root stocks intermediate bundles to be connected with the scions, and then the cactus thorns or toothpicks are used for fixation, bundling and the like through an existing pitaya flush joint method are omitted, and the processes that plastic adhesive is needed to conduct fixation and plastic bag sleeving through an existing pitaya wedge connection method are omitted. By means of the grafting method, accelerated connection and growth of wood between the scions and the root stocks are promoted, operation is simple, time and labor are saved, and the cost of labor and the like is saved by 25% per mu compared with an existing grafting method.

An embodiment of the invention provides a three-dimensional modeling method for a vascular bundle of a corn stalk node, and relates to the field of botany. The method comprises the steps of S1, performing iodine staining processing on a corn stalk node sample to obtain a stained corn stalk node sample; and S2, based on the stained corn stalk node sample, performing Micro-CT scanning to obtain a three-dimensional model of the vascular bundle of the corn stalk node sample. The three-dimensional model of the vascular bundle of the corn stalk node is built through the Micro-CT scanning; paraffin sections are prepared without destroying the shape structure of the corn stalk node; the workload is small; obtained CT scanning image features are consistent; a unified image reconstruction algorithm can be used; and the precision of the obtained three-dimensional model is high.

The invention relates to the plant gene engineering field, particularly a plant leaf promoter conferring vascular-specific expression, a plant expression vector comprising the promoter and a transformant. The invention also relates to the application of the promoter in the plant gene engineering. The nucleotide sequence of the plant leaf promoter conferring vascular-specific expression is shown as SEQ ID No:1. The inventor subjects the plant expression vector with the promoter DNA sequence and a GUS gene to genetic transformation mediated through agrobacterium tumfaciens to obtain transgenic plants. According to identification by immunocytochemistry, the GUS gene only confers vascular -specific expression on rice leaves, therefore, it is supposed that the promoter provided in the invention can drive extrinsic stress-resistant genes to confer vascular -specific expression on plant leaves, so that the promoter can be directionally used for cultivate new transgenic plants with high safety.

The invention provides special bio-organic fertilizer for lotus root planting. The lotus root is an economical crop with a high nutritive value, however, the lotus root rhizome rot is frequently caused due to continuous cropping and mainly happens to a subterraneous stem and roots of the lotus root, thereby causing yellowing, browning and rotting of leaves of the overground part, even withering of the overground part; the initial symptoms of the subterraneous stem are not remarkable, a vascular bundle in the center can be found browning when cut, the browning part gradually expands to a newly grown subterraneous stem, and rotting and blacking of the subterraneous stem are even caused; the leaves of the overground part then have the symptoms, the leaf color fades, the leaves are dried up and curled, and even leaves of the whole field get withered and yellow as if burnt by fire; the subterraneous stem of the diseased or infected plant has hyphae and pink viscous substances, and part of the stem has water-soaking scab; finally, stolon, diseased stems and diseased root parts all have purple brown scab, the stolon gets brown inside, the diseased stem longitudinally shrinks, and the lotus root necrotizes; and the part firstly infected by the lotus root rhizome rot is in an aquatic environment, and the disease cannot be easily found and is difficult to control.

The invention relates to the technical field of bamboo wood mould proofing and corrosion proofing, in particular to an outdoor bamboo wood natural mould-proof corrosion-proof agent and preparation andtreatment methods thereof. The bamboo wood natural mould-proof corrosion-proof agent is prepared from the following components in percentage by weight: 30-40% of industrial wastewater after stevia rebaudiana is processed to prepare stevioside, 5-10% of soapberry fruit, 20-30% of melia azedarach leaf, 10-15% of folium coptis chinensis and 10-20% of folium isatidis. According to the preparation method of the bamboo wood natural mould-proof corrosion-proof agent, conventional decoction or alcohol extraction is adopted; the method for treating bamboo wood by means of the bamboo wood natural mould-proof corrosion-proof agent comprises the steps that after the bamboo wood is placed in hot water for soaking treatment or placed in an ethyl alcohol solution for soaking treatment, the bamboo wood natural mould-proof corrosion-proof agent is used for treatment. The outdoor bamboo wood natural mould-proof corrosion-proof agent and the preparation and treatment methods thereof have the beneficialeffects that firstly, the bamboo wood is placed in the alcohol solution for soaking to make large amounts of starch, sugar, protein and other salt substances in bamboo wood tissues are extracted through alcohol; therefore, the condition that the natural organic mould-proof corrosion-proof agent penetrates through parenchyma cells and vascular bundles of the internal tissues of the bamboo wood is more easy; the mould-proof corrosion-proof agent is prepared from the natural organic extracts and do not have any toxicity or cause pollution problems to the environment.

The invention relates to a method for preparing an anti-leaking vessel made of bamboo and wood materials and a device. The production of the anti-leaking vessel has the following steps: the bamboo and wood materials are made into taking a shape of the vessel and a cylinder body is disinfected and smoothed by steam and put into an oven for drying; a wax absorption treatment is applied to the made bamboo tube by utilizing a vacuum wax absorbing device and the vessel is put into sealant with the temperature of 110 DEG C and is vacuumized or pressurized the inside of the bamboo tube; two minutes later, the vessel absorbs the high-temperature liquid sealant into pores; the vessel is taken out, and a trademark and a label are stuck on the cylinder body, the cylinder body is sheathed with a heat shrinkable film and warehoused after the cylinder body is dried with air. By employing the fluid permeability of the bamboo and wood materials to cause the sealant to permeate into the pores of the materials by pressurization/negative-pressure so as to block the the pores between the vascular bundle structures of the bamboo vessel or block the fluid permeation path, the invention effectively reduces the leakage of packaged products in the vessels(such as the leakage of wine, drinks, etc.) and ensures that the vessel made of the bamboo and wood materials has features of leakage and mould prevention. The invention is beneficial to the storage of the packaged products in the vessel for a long time.

The invention relates to a sample preparing method for corn stalk Micro-CT scanning. According to the method, the related flows of fixing, dehydrating and drying are carried out, it is ensured that a material does not deform, a sample is dehydrated and dried, the sample density difference is increased, the corn stalk sample is prepared successfully, and after micro-CT scanning, a CT image with the clear and bright stalk vascular bundle outline and high quality is obtained. The CT scanning image obtained through the method can be directly used for vascular bundle three-dimensional reconstruction to obtain geometry information and structure information of vascular bundles, and has great significance in construction of a corn vascular bundle multi-scale geometric model and a water transportation dynamic model.

The invention discloses a rooting agent for camellia chrysantha cutting seedlings. According to the formula, the rooting agent comprises the components in percentage by weight: 9% of naphthylacetic acid, 8% of indoleacetic acid, 5% of indolebutyric acid, 8% of aspirin, 5% of vitamin B12, 25% of saccharose, 30% of potassium permanganate and 10% of honey. The rooting agent has the beneficial effectsthat a vascular bundle system is differentiated by using the rooting agent, so that the efficiency of forming adventive roots of the camellia chrysantha cutting seedlings is increased; a 8% aspirin solvent is adopted, so that the rejuvenation periods of the cutting seedlings are shortened, the seedlings are prevented from withering, and the survival rate of the cutting seedlings is increased; therooting agent is prepared by adding cooled boiled water for one-fold diluting of a 5% medical vitamin B12 injection, so that growth of root systems and healing of root tissues are accelerated; the saccharose is brewed into a 25% saccharose solution by using boiled water, and after being naturally cooled, the 25% saccharose solution is added for preparing the rooting agent and is sufficiently stirred, so that the toughness of the root systems is kept higher; and the content of the honey is increased to 10% to ensure that the root systems of the cutting seedlings are attached with a layer of protective film, so that the disease resistance of the cutting seedlings is stronger. The rooting agent has the characteristics of convenience in preparation and long service life.

The invention relates to a rootstock grafting method for rapid rooting and germinating of Pitaya, which belongs to the field of agriculture. The technical scheme adopted includes the following steps:selecting rootstocks; cutting the rootstocks to be not less than 30cm, and obliquely cutting the fleshy stems at the bases to expose 3cm vascular bundles; removing all the thorns on the rootstocks; drying the rootstocks in a cool place for 24 hours; soaking the vascular bundles in compound solution for 48 hours to promote rooting, cutting scion branches to 2.5cm-long and 0.6cm-wide scions, flat-cutting the tops of the rootstocks to expose fresh tissues, making the opposite sides of the scion thorn buds fit the vascular bundles of the rootstocks, using adhesive tapes to tighten and bind the scion thorn buds, and using fresh-keeping films to wrap the top grafting parts of the rootstocks for moisturizing; and inserting the grafted rootstock vascular bundles into wet sand, keeping the room temperature at 30 DEG C, and waiting for 30 days. The rooting of cutting seedlings is promoted and the number of roots is increased through the compound solution, the disease resistance of seedlings is improved by grafting, and the yield of Pitaya is improved.

The invention relates to the technical field of tree bark repairing, in particular to red maple bark damage repairing method. The method includes the steps of a, performing sterilizing and disinsection on a tree body; b, scraping off the rotten part on the damaged part of bark to expose an undamaged layer; c, cutting a slope of 15-45 degrees along the heathy part of the bark towards the damaged part of the bark, wherein the slope cannot have color-changed vascular bundles; d, crushing drepanocladus uncinatus, soaking the crushed drepanocladus uncinatus with mixed solution of 0.25-0.75% carbendazim and 0.04-0.06% naphthalene acetic acid, covering the damaged part with the mixture after swelling of the crushed drepanocladus uncinatus, and fixing and sealing after covering, wherein covering thickness is 1-3cm; e, performing later management. The method has the advantages that the method has great repairing effect on red maple bark damage rotting caused by germs, insects, transportation, human factors and the like, risks caused by covering with mud or paint in the prior art are lowered greatly, and tree survival rate is guaranteed.

The invention discloses a pear lignin synthesis gene PbMC1a/1b and application of the pear lignin synthesis gene PbMC1a/1b in genetic improvement of fruit quality. The PbMC1a/1b gene is a cysteine-like protease isolated and cloned from Dangshansu pear with high lignin content, an applicant names the gene as PbMC1a/1b, the sequence is shown as SEQ ID NO.1, and the corresponding amino acid sequenceis shown as SEQ ID NO.2. A gene construction over-expression vector is introduced into arabidopsis thaliana, obtained transgenic plants are verified by a biological function, the cloned PbMC1a/1b genecan promote the cell walls of fibers between ducts, lignocellulosic fibers and vascular bundles to be obviously thickened, lignin accumulation in stems is promoted and plant growth is inhibited, theexpression of genes related to lignin biosynthesis is increased, new genetic resources is provided for molecular design and breeding of fruit quality, and the PbMC1a/1b gene is an important candidategene for future genetic engineering to improve fruit quality breeding.

The invention discloses a plant promoter related to a photoperiod and an application thereof. The promoter is a DNA molecule of which the nucleotide sequence is as shown in (1), (2), (3) or (4) below: (1) a DNA molecule as shown by nucleotides 1-920 from terminal 5' in sequence 1 of a sequence table; and (2) a DNA molecule of which the nucleotide sequence is one in sequence 1 of the sequence table and contains the following sequence: nucleotides 776-920 from terminal 5' in sequence 1 of the sequence table. The promoter can regulate the photoperiod of the plant, can enable genes not to be expressed in soybean seed coats and mature seeds, and can enable genes to be expressed in vascular bundle tissues. The use of the promoter for genetic breeding can effectively improve the photoperiod and the adaptivity of the plant, and enables the plant to grow in the broader environment; and the promoter is especially applicable to crops, enables the crops to be planted in the broader environment and condition, and improves the variety of crops to raise crop yield. Therefore, the promoter has broad application prospects in the aspect of genetic breeding.

The invention discloses a method for systematically creating watermelon fusarium wilt strains with different pathogenicity and an accurate identification method for resistance to watermelon fusarium wilt. The method for systematically creating the watermelon fusarium wilt strains with different pathogenicity comprises the following steps: respectively creating the strains with different pathogenicity through the technical means of establishing a fusarium wilt mutant library for 4 physiological races of the watermelon fusarium wilt, obtaining genetic transformation mutants of the fusarium wilt, identifying pathogenicity strength of the strains, screening mutant strains with different pathogenicity and the like; and the accurate identification method for the resistance to the fusarium wilt comprises the following steps: carrying out qualitative disease resistance determination on the same material or variety by using the obtained strains with different pathogenicity of the same physiological race, and further being capable of determining resistance strength quantitative disease resistance for a certain physiological race of the same material. The method disclosed by the invention used for identifying the resistance to the fusarium wilt of the watermelon material or variety is simple in steps, produces visual results, and has strong consistency and repeatability; and the method further can be used for other pathogens causing vascular bundle diseases and accelerates resistance identification and disease resistance breeding process of different fusarium wilt host materials and varieties.