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57 results about "Vascular bundle" patented technology

A vascular bundle is a part of the transport system in vascular plants. The transport itself happens in vascular tissue, which exists in two forms: xylem and phloem. Both these tissues are present in a vascular bundle, which in addition will include supporting and protective tissues.

Digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method

The invention provides a digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method, and relates to the technical field of plant phenotype analysis. According to the system, joint coding of cell contours and tissue semantics is achieved through a feature extraction sub-network, scale alignment, global feature convergence and feature re-calibration, and pixel-level cell instance masks and tissue categories are output at the same time through mask decoding and category decoding. In combination with multi-tissue phenotypic parameter classification, in-tissue phenotypic index summarization and structured output, cell-level and tissue-level automatic quantitative analysis of upper epidermis, lower epidermis, fence tissues, sponge tissues, vascular bundles and the like is realized, and the efficiency and precision of multi-tissue cell screening and phenotypic statistics are remarkably improved. The method solves the problems that in the prior art, high-precision automatic segmentation and systematic phenotypic measurement for multi-tissue cells of the cross section of the Chinese cabbage leaf are insufficient, and digital breeding decision making is difficult to directly serve.
Owner:HEBEI AGRICULTURAL UNIV.

Application of phb9 gene in regulating rice leaf morphology

PendingCN122648439ABiotechnologyVascular bundle
The application discloses application of a PHB9 gene in regulating rice leaf morphology and belongs to the technical field of genetic engineering.The CDS sequence of the PHB9 gene is shown as SEQ ID NO.2.It is found through experiments that overexpression of the PHB9 gene increases leaf length and leaf width, and the PHB9 gene has a significant effect on regulating rice leaf morphology.Further, changes in vascular systems, mitochondria and chloroplast structures in the internal structure of the leaf are analyzed, and it is shown that the vascular systems, mitochondria and chloroplast structures change when the leaf morphology is regulated, and the morphological changes in the internal structure of the leaf affect the changes in the leaf phenotype, and have a significant influence on improving the photosynthetic rate of the leaf.It is also found that overexpression of the PHB9 gene reduces the sensitivity of rice seeds to ABA, promotes seed germination and root growth.The application provides a new gene for breeding of leaf morphology building, root development and seed germination.
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI

Strawberry freezing method

This invention provides a method for freezing strawberries that can reduce the amount of drip that occurs when frozen strawberries are thawed. [Solution] As preparation, a solution containing an ingredient that solidifies into a jelly-like substance at room temperature is prepared. In step 1, strawberries are placed in a vacuum container and the pressure is reduced, causing moisture from inside the strawberries to seep out to the surface through the vascular bundles. In step 2, while maintaining the reduced pressure, the solution containing the ingredient that solidifies into a jelly-like substance at room temperature is brought into contact with the surface of the strawberries. In step 3, with the surface of the strawberries covered with the solution containing the ingredient that solidifies into a jelly-like substance at room temperature, the reduced pressure is released, filling the openings of the vascular bundles on the surface of the strawberries with the solution. In step 4, the strawberries after step 3 are rapidly frozen.
Owner:卜部 俊郎

3D printing high-energy-absorption bionic bamboo vascular bundle and preparation method thereof

The invention discloses a 3D printing high-energy-absorption bionic bamboo vascular bundle and a preparation method thereof, and belongs to the technical field of impact protection. The energy absorption pipe is integrally formed through the additive manufacturing technology, the pipe wall configuration of the energy absorption pipe simulates a natural bamboo vascular bundle system, and a three-dimensional grid structure with longitudinal discontinuous holes and transverse reinforcing ribs is innovatively designed. A PETG / carbon fiber composite material is preferably selected as a manufacturing material of the energy absorption pipe, the printing layer height is optimized, and cooperation of the structure, the material and the process is achieved. The problems that a traditional energy absorption structure is too high in initial peak force and insufficient in light weight are effectively solved, and the energy absorption pipe is excellent in comprehensive performance and suitable for the fields of vehicle collision protection, aerospace buffering and the like.
Owner:JIANGSU AVIATION VOCATIONAL & TECH COLLEGE

Vascular bundle targeting biological nano-selenium and preparation method and application thereof

This invention discloses a vascular bundle-targeted bio-selenium nanoparticle, its preparation method, and its application, belonging to the field of plant treatment technology. The invention involves mixing and dissolving bio-selenium nanoparticles, water, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide, followed by activation to obtain an activated bio-selenium nanoparticle solution. A xylem-anchored peptide and MES buffer are then mixed and dissolved to obtain a xylem-anchored peptide solution. The activated bio-selenium nanoparticle solution and the xylem-anchored peptide solution are mixed, and the pH is adjusted to 6.3-6.8 before stirring to obtain the vascular bundle-targeted bio-selenium nanoparticle. The vascular bundle-targeted bio-selenium nanoparticle provided by this invention achieves interception of heavy metal transport pathways by specifically anchoring to xylem vessels. Compared with existing technologies that rely solely on broad-spectrum physiological antagonism, its efficiency in reducing heavy metal content in crop stems and leaves represents a qualitative leap.
Owner:SELENIUM TRAVEL NOTES (SHENZHEN) SYNTHETIC BIOTECHNOLOGY CO LTD

Chinese orchid bud-promoting and seedling-raising method and bud-promoting and seedling-raising system

The invention belongs to the technical field of bud promoting and seedling raising, and discloses a Chinese orchid bud promoting and seedling raising method, which is characterized in that three radial cutting lines forming 120-degree included angles with one another are manufactured along the longitudinal axis direction of a pseudobulb by cutting from the base of an epiphytic leaf at the top end of the pseudobulb of an orchid seedling. The cutting depth is 30% of the radial thickness of the pseudobulb (1t; 1 / 3 of the radius of the pseudobulb). While the pseudobulb is effectively stimulated to generate calluses and germinate new buds, the original vascular bundle system, leaf buds and root bases of the pseudobulb are protected from structural damage to the greatest extent. The incision can absorb hormone more easily, and the germination rate is increased. The orchid plants subjected to hormone treatment are comprehensively sprayed and disinfected with 600-fold 75% chlorothalonil liquid for three times, spraying each time needs to be carried out after the last time of liquid medicine is completely dried, it is ensured that pseudobulbs and incisions are covered in place, the situation of germ infection at the incisions is prevented, and the survival rate is increased.
Owner:BEIJING VOCATIONAL COLLEGE OF AGRICULTURE (PARTY SCHOOL OF RURAL WORK COMMITTEE OF BEIJING MUNICIPAL COMMITTEE OF THE COMMUNIST PARTY OF CHINA)

Method for promoting regeneration and growth of tissue culture seedlings by cultivating larch tissue culture seedlings and combining larch tissue culture seedlings with soil culture seedlings to establish vascular bundle communication

The invention relates to the technical field of tissue culture, in particular to a method for cultivating larch tissue culture seedlings and combining the larch tissue culture seedlings with soil-planted seedlings to establish vascular bundle communication to promote regeneration and growth of the tissue culture seedlings. Unrooted larch tissue culture seedlings are used as in-vitro culture seedling jointing units, one-year-old larch seedlings are used as root system supply units and the like, the in-vitro culture seedling jointing units and the root system supply units are tightly attached, then upper openings and lower openings of rubber pipes are sealed, and combined plants are obtained; and putting the combined plant into a sealed box, culturing for 40 days, moving out of the sealed box, and continuously culturing for 40 days. The limitation that rooting and transplanting are difficult in the larch tissue culture regeneration system process is broken through, the regeneration system is more perfect, and the culture time is saved. A feasible scheme is provided for breeding of a precious new variety of larch, and the method is also a breakthrough in a larch breeding technology level. Meanwhile, a technical support is provided for subsequent development of genetic improvement and industrial application of larch trees.
Owner:NORTHEAST FORESTRY UNIV

A nano-bandage for preserving cut flowers

This invention discloses a nano-bandage for preserving cut flowers, belonging to the field of horticultural technology. The nano-bandage consists of a polymer carrier and a bactericide encapsulated therein. The polymer is a mixture of PEG and PDMAEMA. By adjusting the mass fraction and total concentration of PDMAEMA, the particle size of the nanoparticles can be controlled, and their surface can be positively charged. It is prepared using microfluidic co-assembly technology, where the drug-containing organic phase and aqueous phase are instantaneously mixed and purified by dialysis. This nano-bandage actively targets and persistently accumulates on the negatively charged cut surface of fresh cut flowers using electrostatic interactions, forming a physical barrier. It also synergistically inhibits microbial infection through slow-release bactericide and charge adsorption, while its nano-size does not affect vascular function. This invention achieves precise and long-lasting protection for cut flower wounds, significantly extending vase life with extremely low dosage, and has the advantages of high efficiency, safety, and intelligent adjustability.
Owner:EAST CHINA UNIV OF SCI & TECH +1

Cutting mechanism and grafting machine comprising same

The utility model discloses a cutting mechanism and a grafting machine comprising the same. The cutting mechanism comprises a supporting base, the supporting base is provided with a transversely-extending cutting groove, and the cutting groove is used for containing a plant body; the cutting knife can move up and down relative to the cutting groove, the cutting knife is provided with a knife edge, the knife edge faces the cutting groove, and the cross section of the cutting knife in the horizontal direction is inclined to the extending direction of the cutting groove. Therefore, on one hand, the extending direction of the cutting groove is roughly the same as that of the plant body, the natural growth direction of the plant body can be kept when the plant body is placed due to the design, and the problem that vascular bundles are damaged due to the fact that the cutting direction is inconsistent with the growth direction of the plant body is solved; and the cross section of the cutter in the horizontal direction is inclined to the extension direction of the cutting groove, so that the cutter can cut in a softer and gradual manner when cutting a plant body, and the damage to vascular bundles of the plant body is effectively reduced.
Owner:HEYUAN CHENS HUAXING AGRICULTURAL DEVELOPMENT CO LTD

Application of ZmERF75 gene in regulation and control of corn biomass traits and improvement of straw saccharification efficiency

The invention discloses an application of a corn ERF transcription factor ZmERF75 in regulation and control of corn stalk development and cell wall composition. Researches find that by reducing the expression of the ZmERF75 gene (such as a zmerf75 mutant), the plant height, the stem diameter, the stem bending force and the leaf tension of the corn can be obviously reduced, and vascular bundles are sparsely distributed. Meanwhile, the stem cellulose content of the zmerf75 mutant is reduced, the lignin content is increased, the monosaccharide content is changed, and the saccharification efficiency is remarkably improved. An EMSA and yeast single hybridization experiment proves that the ZmERF75 protein can be specifically combined with DNA in a CUI gene promoter region. The invention discloses a key regulation and control effect of ZmERF75 in corn stalk development and cell wall synthesis, and provides a new gene target and theoretical basis for improving the biomass utilization efficiency of corn stalks, optimizing cell wall composition and improving stalk characters through a molecular breeding means.
Owner:QINGDAO AGRI UNIV

Bacterial liquid preparation for promoting healing of grafting unions of muskmelon and pumpkin and application of bacterial liquid preparation

The invention relates to a plant grafting technology in agricultural horticulture, in particular to a bacterial liquid preparation for promoting healing of grafting unions of muskmelons and pumpkins and application of the bacterial liquid preparation, and provides a bacterial liquid preparation which is simple in operation method, outstanding in application effect and capable of promoting healing of grafting unions of the muskmelons and the pumpkins according to physiological and molecular mechanisms of CmGH9B3 infection liquid in grafting healing of the muskmelons and the pumpkins. The bacterial liquid preparation can significantly improve the grafting healing efficiency of muskmelon and pumpkin and the quality of grafted seedlings. In the attaching grafting process of the muskmelon and the pumpkin, the muskmelon scion hypocotyl is soaked in a bacterial liquid preparation to be infected for 30 min, the muskmelon scion hypocotyl and the pumpkin rootstock are fixed through a grafting clamp, callus formation at a grafting opening and connection between the scion and a vascular bundle bridge of the rootstock can be promoted, and then healing of the grafting opening of the muskmelon and the pumpkin is accelerated; the grafting healing rate and survival rate are improved, and the grafted seedling quality is guaranteed.
Owner:SHENYANG AGRI UNIV

Application of RanGAP in regulating symbiotic nodulation

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.
Owner:CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI

Combined artificial vascular sheath capable of reconstructing lymph node motive force, guiding device, and use method

PCT designated stageWO2026025518A1Blood vesselsVascular bundleHuman body
Disclosed are a combined artificial vascular sheath capable of reconstructing lymph node motive force, a guiding device, and a use method. The combined artificial vascular sheath comprises an elastic sheet and a plurality of tying bands connected to the elastic sheet, wherein the elastic sheet covers a vascular bundle, and the plurality of tying bands sequentially surround and bundle the vascular bundle to form a combined artificial vascular sheath wrapping the vascular bundle. The guiding device comprises a retractor, wherein the retractor is of an arc-shaped plate structure, the front end of the retractor is provided with a guide hook, and the guide hook matches a hook hole on the tying band and the opening thereof faces backwards. According to the present invention, the combined artificial vascular sheath with low elasticity is arranged on the surface of the vascular bundle to replace the damaged or hardened vascular adventitia which has lost its function, such that the function of squeezing and stimulating the peripheral lymph nodes of the blood vessel is reconstructed, and the influence on blood vessel branches is also reduced. The guiding device enables operators to conveniently implant the combined artificial vascular sheath, and has the advantages of simple structure, convenient operation and little damage to human bodies.
Owner:SHINEYARD MEDICAL DEVICE CO LTD

Plant vascular bundle bionic directional wick and manufacturing method thereof

The invention discloses a plant vascular bundle bionic directional wick and a manufacturing method thereof, and relates to the technical field of wick, the plant vascular bundle bionic directional wick comprises a matrix and a plurality of diversion layers; the base body is provided with a circulation area; the multiple flow guide layers are arranged in the circulation area in the height direction of the base body; the flow guide layer comprises a plurality of guide strips, and a micro-channel is formed between every two adjacent guide strips. The guide strips are arranged to simulate vascular bundle structures in plants, a micro-channel is formed between every two adjacent guide strips, and under the action of capillary force, a liquid working medium located in a circulation area moves in the channels; the guide strips of every two adjacent flow guide layers are arranged in a staggered mode, on one hand, the capillary effect can be enhanced, the working medium is accelerated to flow from one end to the other end of the circulation area, and on the other hand, the working medium flowing direction can be controlled through the staggered structure design; a strong capillary force is generated through a simple structure, the purpose of controlling liquid to flow in the set direction is achieved, and long-distance conveying of a working medium is achieved.
Owner:GUANGZHOU UNIVERSITY

Desalting device and in-situ desalting system

The invention provides a desalting device and an in-situ desalting system, and the desalting device comprises a bamboo pipe which is provided with a hollow cavity, and the two ends of the hollow cavity are arranged in a through manner along a first direction; the multiple permeation holes are formed in the bamboo pipe, one end of each permeation hole communicates with the hollow cavity, the other end of each permeation hole penetrates through the outer wall of the bamboo pipe, at least one permeation hole extends in the second direction, the second direction intersects with the first direction, and the multiple permeation holes are formed in the bamboo pipe, so that salt-containing water can enter the bamboo pipe through the multiple permeation holes; meanwhile, due to the capillary action of the vascular bundle structure, salt-containing water can be driven to migrate into the bamboo pipe, salt is gradually accumulated and crystallized in the pipe wall and the hollow cavity, the water can be evaporated into the atmosphere through the end opening of the bamboo pipe, and therefore upward pulling force is generated, migration power of the salt is increased, and the salt content is increased. And therefore, the problem of radial permeation of the bamboo is solved, and meanwhile, the salt catching amount per unit volume is remarkably increased.
Owner:BEIJING FORESTRY UNIVERSITY

Use of miR396f and / or grf7 related biologicals in improving heat tolerance in plants

This invention belongs to the field of plant molecular biology and genetic engineering technology, specifically involving miR396f and / or GRF7 The application of related biological products in improving plant heat resistance. This invention confirms... miR396f This is a heat-inducible miRNA primarily located in the vascular bundles. Its promoter exhibits sequence polymorphisms in the heat-resistant variety HT54 and the heat-sensitive variety HT14, leading to differences in heat stress responses. Through a multi-step screening strategy, this invention identifies and validates for the first time... GRF7 for miR396f A direct target of the rice heat tolerance pathway. Genetic evidence suggests that... miR396f Through negative regulation GRF7 Positive regulation of heat tolerance during the seedling and reproductive stages of rice. This invention discloses... miR396f - GRF7 The module provides important genetic resources for breeding heat-resistant crops.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Mokuzaidori air dryer

To increase a speed in drying wood.SOLUTION: Ventilation is performed so as to pass through the vascular bundle from the end of the wood.SELECTED DRAWING: None
Owner:吉田 裕昭

A method for propagating mangoes by air layering

This invention proposes a method for air layering propagation of mangoes, belonging to the field of mango propagation technology. Mango propagation is mainly carried out through grafting or top grafting, which is highly dependent on the rootstock. In production, inappropriate rootstock selection can easily lead to the underutilization of superior varietal characteristics. The air layering method of this invention involves cutting a branch with xylem into slices, applying rooting powder, and then inserting it into a container with water. The mineral nutrients and water absorbed from the roots can still be transported to the branches above the cut through the branch's vascular bundles. Furthermore, the photosynthetic products of the leaves above the cut are transported to the wound to form callus tissue and promote root growth, resulting in rapid root development. This mango air layering propagation method preserves the genetic characteristics of the mango tree. This mango air layering propagation method of this invention has advantages such as low cost, simple operation, short time, high survival rate, and no need to consider whether the air-layered branches easily separate from the bark.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Method for improving soil based on bamboo medium farming method

A method for improving soil based on a bamboo medium farming method comprises the steps that 1, bamboo fiber vascular bundle mincing processing is conducted on bamboos through a fiber breaking crusher, fiber removal is achieved, and fiber-removed bamboo powder is obtained; step 2, adding a composite biological enzyme MP16 into the defiberized bamboo powder, and performing composite fermentation to prepare a bamboo medium organic fertilizer; 3, the bamboo medium organic fertilizer is scattered on the surface of the soil, and the thickness is about 2-3 cm. The soil is treated and improved through a microbial technology; the microorganisms in the soil are subjected to compound fermentation and circulation, so that the probiotics have dominant advantages, the soil is soft and nutritious, and the soil environment quality is gradually improved.
Owner:WUPING COUNTY CARBON CHAIN ZHIKE ECOLOGICAL TECHNOLOGY CO LTD

Method for manufacturing bamboo toothbrush and bamboo toothbrush produced thereby

A bamboo toothbrush manufacturing method may include an operation of preparing raw materials, which selecting some of the raw materials as materials suitable for manufacturing bamboo toothbrushes on the basis of a vascular bundle ratio on the cross-section of each of the raw materials; a milling operation of processing the selected materials into the shapes of toothbrush bodies; a pretreatment operation of placing the materials, which have been subjected to the milling process, in an environment having a certain temperature range and a certain humidity range for a certain time range; a hot pressing operation of inserting the materials into a press die, and heating and pressing the materials; an operation of sanding and coating the materials; a drilling operation of forming a plurality of holes in a head portion of each of the materials; and an operation of planting toothbrush bristles into the plurality of holes.
Owner:PROJECT NOAH INC

Method for monitoring tomato fusarium wilt and early prevention and treatment based on minimally invasive tissue sampling

The invention belongs to the field of biotechnology research, and describes a method for monitoring tomato fusarium wilt and early prevention and treatment of diseases based on minimally invasive tissue sampling. The method comprises the following steps: puncturing a tomato stem base through a sterile syringe needle to obtain trace vascular bundle tissues, and performing rapid nucleic acid extraction; then, whether the plant is infected or not is judged 5-7 days before typical symptoms appear through an isothermal amplification nucleic acid detection technology, the positive detection rate of a loop-mediated nucleic acid isothermal amplification (LAMP) detection method is 47.6%, and the positive detection rate of a recombinase-mediated nucleic acid isothermal amplification (RAA) detection method is 89.71%; finally, for the detected positive plants, in the window period when disease symptoms do not appear, trifluoropyridylamine and bacillus velezensis JSBV63 are adopted for root irrigation treatment in time, the progress of plant diseases can be controlled and blocked, and the control effect can reach 90% or above after two times of pesticide application. The combination of minimally invasive sampling, isothermal nucleic acid amplification detection and medicament treatment realizes a whole set of complete fusarium wilt prevention and treatment technical process from early diagnosis of pathogen infection to disease treatment.
Owner:JIANGSU ACAD OF AGRI SCI

Bionic diffusion growth driven stiffened plate shell anti-buckling topology design method

The invention relates to the design of a thin shell reinforced structure, and discloses a bionic diffusion growth driven anti-buckling topology design method of a reinforced plate shell. According to the design method, an initial shell finite element model is constructed based on the growth characteristics of the vascular bundle of the blade, and buckling analysis is carried out by utilizing a base structure modeling scheme. Establishing an optimization column formula, calculating feedback according to the objective function, the design variable and the constraint function, and taking the structure initial response as an initial value of the optimization column formula; setting an initial growth node and a convergence condition; and a diffusion type reinforced active node updating strategy is set, diffusion type growth optimization is carried out, and the iteration process is shortened. According to the invention, the flexibility of BRD design is effectively improved, so that the structure has efficient anti-buckling bearing capacity, and the research and development cycle of products is shortened.
Owner:SUZHOU UNIV OF SCI & TECH

Portable micro-pressure perfusion device for vascular bundle injection of plants

ActiveCN224402303USolve the steps that require repeated manual aspiration of biological agentsImprove portabilityBiotechnologyVascular bundle
The application discloses a portable micro-pressure perfusion device for plant vascular bundle injection and belongs to the technical field of micro-pressure perfusion devices. The micro-pressure perfusion device comprises a perfusion device cylinder and a piston rod slidingly connected in the perfusion device cylinder, and the micro-pressure perfusion device is detachably connected with a liquid storage tank. The whole design structure is portable and flexible to use. By holding and releasing the pressure holder, one-time plant vascular bundle perfusion operation can be completed, and one-time automatic absorption of biological medicaments from the liquid storage tank into the perfusion device cylinder can be completed. Through repeated operations, the above operation can be realized for multiple plant vascular bundle injection. The micro-pressure perfusion device solves the problem that the traditional micro-pressure perfusion device needs to repeatedly manually absorb biological medicaments in the process of multiple plant vascular bundle injection, greatly improves the injection efficiency, and through rotation of the adjusting nut, the capacity of the perfusion device cylinder for absorbing biological medicaments at one time can be quickly adjusted, the adjustment of the capacity of biological medicaments for each time of perfusion is realized, and the micro-pressure perfusion device is convenient and practical to use.
Owner:SHIHEZI UNIVERSITY

Method for increasing drought tolerance by accumulation of plant osmoregulators

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.
Owner:GUILIN UNIVERSITY OF TECHNOLOGY

Grafting method for accurately applying pre-tightening force to scions and stocks

The invention discloses a grafting method for accurately applying pre-tightening force to scions and stocks, which comprises the following steps of: firstly, realizing stable clamping and axis alignment of the stocks and the scions through a first clamping jaw and a second clamping jaw which are axially arranged; an optimal pre-tightening force threshold value is set according to the hardness difference of stems of the stock and the scion, and a pneumatic and electric combined mode is adopted to realize a pneumatic-electric two-stage butt joint mode, so that a diagonal plane of the scion and a diagonal plane of the stock are in butt joint to realize accurate and tight fitting of cutting surfaces of the stock and the scion; in the attaching stage, the pre-tightening force is fed back and adjusted in real time by means of a pressure sensor, the vascular bundle is prevented from being damaged by too large pre-tightening force or untight attaching caused by too small pre-tightening force is avoided, and the problems that in traditional grafting, the vascular bundle attaching degree is poor, and the pre-tightening force is controlled by experience are solved.
Owner:TIANJIN AGRICULTURE COLLEGE

Directional activated modified charcoal based on green bristlegrass silicon-rich-vascular bundle structure, preparation method and application thereof

The invention belongs to the technical field of environmental functional materials and advanced treatment of antibiotic wastewater, and particularly relates to directionally activated and modified biochar based on a green bristlegrass silicon-rich-vascular bundle structure as well as a preparation method and application of the directionally activated and modified biochar. The preparation method comprises the following steps: cleaning green bristlegrass, drying, crushing and sieving; dipping green bristlegrass powder in a KOH solution, initiating pre-activation, and drying after dipping is finished; in an inert atmosphere, pyrolyzing the impregnated sample; and finally, sequentially carrying out acid pickling, water washing, re-drying, crushing and sieving on the pyrolyzed sample to obtain the directionally activated and modified charcoal. The preparation process is simple, and the cost is low; the prepared material has a high specific area, is mainly mesopores, is rich in oxygen-containing functional groups on the surface, can be used as a high-selectivity and high-capacity tetracycline adsorption material, and can keep stable adsorption performance in a wide pH range. The method disclosed by the invention realizes the dual goals of waste recycling and antibiotic deep purification, and has extremely high industrialization value and application prospect.
Owner:SHANGHAI SECOND POLYTECHNIC UNIVERSITY

Soybean alkali-inducible promoters and uses thereof

The soybean alkali-inducible promoter and application thereof belong to the technical field of genetic engineering. In order to mine the soybean alkali-inducible promoter, the present application finds through research that GmWRKY53 The gene is significantly induced by alkali stress, and overexpression of the gene can significantly improve the alkali stress resistance of soybean. GmWRKY53 The promoter of the gene pGmWRKY53 Can drive the specific expression of a reporter gene in the root vascular bundle of soybean, and is an alkali-inducible promoter. Compared with the use of a constitutive promoter p35S To drive GmWRKY53 The overexpression of the gene, the use of pGmWRKY53 To drive GmWRKY53 The overexpression of the gene can improve the survival rate of the transgenic strain under alkali stress at the seedling stage by more than 20%. The soybean alkali-inducible promoter provided by the present application pGmWRKY53 Can be used for breeding alkali-resistant transgenic soybean, improving the salt-alkali stress resistance of soybean, and further improving the yield of soybean planted in saline-alkali land.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Genes, recombinant vectors and uses for regulating flowering in plants

ActiveCN116254273Bshorten flowering cyclePlant peptidesFermentationBiotechnologyVascular bundle
The application belongs to the field of plant growth and relates to a gene for regulating flowering and disease resistance of plants, in particular to a gene for regulating flowering, a recombinant vector and application. In the research process of disease resistance breeding, a NBS-LRR type gene of Nicotiana benthamiana is cloned by the research group NbLOV1 . Bioinformatics analysis results show that the gene is closely related to the response of plants to fungal pathogens, and in the experiment of verifying the gene function by gene editing knockout, the plants created by using the knockout vector for NbLOV1 and the Agrobacterium-mediated transformation method have obvious differences compared with the control: after knocking out NbLOV1 , the budding and flowering time of Nicotiana benthamiana is obviously earlier than that of the control. The mutant plant with the knockout NbLOV1 gene presents strong resistance to fusarium, only with slightly yellow leaf color and slight wilting; while the tobacco of the control presents damaged vascular bundle, seriously wilting leaf surface and necrotic spots.
Owner:TOBACCO RES INST HENAN ACADEMY OF AGRI SCI

Cellular morphology and moisture distribution-based fresh ginger brittle variety identification method

The invention relates to the technical field of variety identification, and provides a fresh ginger brittle variety identification method based on cellular morphology and moisture distribution, and the method comprises the following steps: S1, selecting a fresh ginger variety to be identified, and planting the fresh ginger variety according to a unified cultivation management condition until harvesting; s2, during harvesting, selecting to-be-identified ginger varieties which are consistent in plant type and are not diseased, taking tender ginger parts, and detecting the brittleness value, the transverse relaxation time of bound water, the average cell area and the average vascular bundle area; s3, single score conversion: converting the detection values into corresponding single scores according to a preset five-level segmentation scoring rule; and S4, comprehensive discrimination: comprehensively discriminating the brittleness of different varieties of fresh gingers by adopting a weighted scoring function. The invention establishes a combined identification method of cellular morphology and NMR moisture distribution, realizes accurate and objective identification of the high-brittleness ginger variety, and provides technical support for germplasm resource protection, origin identification management and raw material quality control.
Owner:LAIFENG COUNTY URBAN CONSTR INVESTMENT CO LTD +1

Application of Gn1a new alleles in rice yield improvement

The invention discloses application of Gn1a new alleles in rice yield improvement, and belongs to the field of gene engineering. The technical problem to be solved by the invention is how to improve the yield of rice. Therefore, the invention provides the application of the protein or a substance for regulating the expression of a protein coding gene or a substance for regulating the activity or content of the protein in regulating the plant yield, and the protein is the protein with the amino acid sequence of SEQ ID No.3. Gn1a new allelic homozygous mutation rice mutants are obtained through gene editing. Phenotype verification shows that the yield traits of the rice mutant, including the unit yield, the number of effective panicles in a plot and the total number of grains per panicle in the plot, are obviously higher than those of a wild type. The invention discloses the application of the Gn1a new allelic in regulation and control of the rice yield, the panicle type and the vascular bundle character for the first time, and proves that the Gn1a new allelic has a remarkable regulation and control effect in regulation and control of the rice yield, the panicle type and the vascular bundle character, and can be used for rice breeding.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI