25 results about "Transcription start" patented technology

The invention discloses a plukenetia volubilis FAD2 gene promoter. The nucleotide sequence of the plukenetia volubilis FAD2 gene promoter proPvFAD2.4 is shown in the specification. The proPvFAD2.4 comprises TATA-box and CAAT-box, and the TATA-box and the CAAT-box are located at the upstream of a transcription start site (TSS), so that basic transcription support is provided for positioning of RNA polymerase II, and the basic expression level of the gene is ensured. Comprising seed specific elements of AE-box and O2-site, and specific expression of PvFAD2 in the seed development stage is combined with DOF (OBP2) and MADS-box transcription factors to drive grease to be quickly accumulated. And the recombinant plasmid vector has a strong starting effect, so that the promoter has a function of starting downstream gene expression in a tobacco leaf cell environment, and an experimental foundation is laid for subsequent analysis of a regulation mechanism of the promoter.

The application belongs to the technical field of biological gene engineering, and particularly relates to a barley HvCslf6 promoter element and application thereof in regulating the content of barley grain beta-glucan, wherein the barley HvCslf6 promoter element is located at 295bp-300bp upstream of a transcription start site of a barley HvCslf6 gene; the nucleotide sequence of the HvCslf6 promoter element is CCGTTG, and the HvCslf6 promoter element belongs to a CCAAT-box element; the gene number of the HvCslf6 gene in BARLEX Morex v3 Gene Models is HORVU.MOREX.r3.7HG0698110. The application finds a key element which can affect the content of beta-glucan in barley grains in the upstream promoter region of a main effect gene HvCslf6 of barley beta-glucan synthesis by using a gene editing technology, and thus provides a method capable of preparing transgenic plants with changed content of beta-glucan in barley grains, and provides a new means and tool for detecting and regulating the content of beta-glucan in barley grains.

The integration procedure provides different elements that require in vitro amplification of HSPCs, preferably for about 48 hours, in the presence of hematopoietic cytokines as well as UM171 and stem cell regenerative factor 1, and the introduction of i) a system (e.g., a CRISPR / Cas9 system) that generates DNA double-strand breaks (DSBs) upstream of the transcription start site of the target gene, and ii) the introduction of a donor matrix comprising cDNA with homologous arms on both sides (left [LHA] and right [RHA]).

Methods for increasing expression of a target gene, the method comprising introducing a CCCTC-binding factor (CTCF) binding site (CTCF-BS) into a promoter region of the target gene, e.g., within 500, 250, 200, 150, 100, 50, or 25 nucleotides of the transcription start site (TSS) for the target gene, and optionally expressing in or introducing into the cell a CTCF protein or variant thereof.

The invention relates to a cancer non-invasive early screening method and system based on sequencing coverage depth and fragment size characteristics near a cfDNA transcription start site.

The application provides a non-coding mutation-induced risk gene expression up-regulation and down-regulation prediction method and system, comprising the following steps: step 1, obtaining a data set and performing pretreatment; step 2, information input standardization between non-coding mutations and gene transcription start points TSS; step 3, non-coding mutation information input standardization; step 4, sequence length alignment and representation learning framework; and step 5, embedded feature merging and prediction output. The application is matched with chromatin accessibility sequencing data to migrate to any tissue or cell type, make accurate and reliable prediction, and utilize an attention mechanism to endow the model with interpretability.

Guide RNAs and CRISPR / Cas systems targeting a C9orf72 gene, lipid nanoparticles or viral vectors comprising such CRISPR / Cas systems, and cells or animals comprising such CRISPR / Cas systems are provided. Methods of repressing transcription from a C9orf72 exon 1 A transcription start site and / or repressing transcription of sense and / or antisense transcripts that comprise the hexanucleotide repeat expansion sequence in a C9orf72 gene using the CRISPR / Cas systems are also provided, as well as use of the CRISPR / Cas systems in prophylactic and therapeutic applications for treatment and / or prevention of a C9orf72 hexanucleotide repeat expansion associated disease and / or for ameliorating at least one symptom associated with such disease.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.

Provided are a tissue tracing method for cell-free DNA, a tissue lesion risk notification method, a cell-free DNA-based cancer risk indication method, a cell-free DNA-based gestational disease risk indication method, a cell-free DNA-based receptor tolerance risk notification method, a disease treatment effect evaluation or prognosis method and apparatus, an electronic device, a computer-readable storage medium, a computer program product and a computer program. The tissue tracing method for cell-free DNA comprises: acquiring a tissue-specific expression gene set of a tissue; acquiring first distribution information of cell-free DNA derived from a sample on specific regions of genes in the tissue-specific expression gene set; and, on the basis of the first distribution information, acquiring a tissue contribution index of the tissue in the cell-free DNA, so as to trace the tissue of the cell-free DNA, wherein the specific regions comprise transcription start site (TSS) regions.

This invention belongs to the field of plant molecular biology and genetic breeding technology, specifically relating to a molecular marker related to the malic acid content of apple fruit and its application. The molecular marker is... MdNAC029 The single nucleotide polymorphism site in the gene promoter region, this site is located MdNAC029 The nucleotide sequence of the gene promoter region, starting from the 2693rd base at the 5' end, corresponds to... MdNAC029 At a position 442 bp upstream of the transcription start codon, the polymorphism is either T or C, with the C / C genotype indicating high malic acid content and the T / T genotype indicating low malic acid content. This invention, through molecular biology experiments, confirms that the T allele is the binding site for the transcriptional repressor MdMYC2, and the C allele eliminates the binding of MdMYC2, thereby relieving transcriptional repression and promoting malic acid accumulation. This provides a new molecular marker for the genetic improvement of apple fruit acidity traits, accelerating the breeding process of superior varieties.

The invention relates to a sample treatment method for detecting accessibility of single cell chromatin and application of the sample treatment method. The sample treatment method comprises the following steps: slicing a tissue sample to obtain a tissue slice, and sequentially carrying out permeabilization treatment, transposase treatment and cell nucleus extraction treatment on the tissue slice, the tissue sample includes a frozen tissue sample. According to the method, a'slice in-situ transposition reaction 'strategy is designed, the original spatial conformation and physiological state of chromatin in vivo are effectively maintained, chromatin structure disturbance caused by early release of cell nucleuses in the thawing process is avoided, the quality of ATAC-seq data is remarkably improved, and the method is suitable for being used in a large scale. The enrichment fraction of the transcription start site and the proportion result of fragments overlapped with peaks are remarkably improved, a nucleosome positioning signal is clearer, and the accuracy and reliability of chromatin openness detection are improved.

An engineered nucleic acid for regulating levels of gene expression, including in cells is provided. The nucleic acid has a regulatory region with a transcription factor binding site upstream of a transcription start site and separated by a spacer and includes at least one of multiple distinct transcription factor binding sites, a recombination regulation system and / or nested excisable spacers and orthogonal recombination sites. Methods of regulating gene expression are also provided.

The application discloses a method for eRNA transcription identification, regulation target prediction and function annotation based on high-throughput transcriptome sequencing data, and is characterized in that the method comprises the following steps: identifying part of non-coding RNA in which a transcription start site is located in an enhancer region as eRNA; obtaining eRNA-related protein coding genes which simultaneously exist in an eRNA-protein coding gene co-expression network and an eRNA-centered regulation network, constructing an eRNA-protein coding gene relationship network, and extracting protein coding genes which are directly connected or closely connected to the eRNA, so as to predict potential regulation targets of the eRNA; and finally, performing function enrichment analysis on the potential regulation targets of the eRNA, so as to obtain the results of eRNA function annotation, and the method has the advantages of wider application range, and can be applied to all eRNAs and more accurately obtain the action forms between the eRNA and the protein coding gene.

Methods and compositions are provided for using transcription start site (TSS) profiling to identify alternate promoters that yield better transcription modulation (e.g., knockdown using CRISPRi or activation using CRISPRa). Methods can also include designing CRISPR-Cas guide RNAs to target CRISPRi or CRISPRa complexes to the identified alternative promoters, and methods can also include steps of generating such guide RNAs. Also provided are libraries of guide RNA, methods of modulating expression of a target gene. In some cases, multiple promoters for the same gene are targeted simultaneously.

Provided herein are isolated nucleic acids, vectors, recombinant cells, and transgenic animal tremor longimanus capable of expressing at least one or more tremor longimanus genes. Also provided are recombinant cells and transgenic animals comprising one or more abrupt hair gene-specific deletions in the regulatory region upstream of the identified transcription initiation site.

A method for increasing gene expression and improving herbicide resistance in plants. Specifically, the method involves performing modifications at upstream and downstream regions of a transcription start site of a gene of interest by means of a genome editing technique, so as to modify a non-coding region of the gene, thereby increasing the expression level of an endogenous polynucleotide or the gene of interest. Furthermore, the method is used to modify and regulate an HPPD gene and / or HIS1 gene associated with herbicide resistance in the plants, thereby improving herbicide resistance in the plants.

The present invention relates to synthetic enhancer elements that can be used to increase the expression of a gene of interest in any plant tissue. The disclosed synthetic enhancer elements can be used in a gene expression cassette comprising nucleic acid further comprising a promoter and a sequence to be expressed, wherein the sequence to be expressed has a transcription start site, and wherein the synthetic enhancer element is operably linked to the promoter and the sequence to be expressed. The present invention also relates to a modified plant or part thereof comprising a synthetic enhancer element, wherein the synthetic enhancer element is operably linked to an endogenous promoter in the modified plant or part thereof and an endogenous gene in the modified plant or part thereof, and wherein the synthetic enhancer element increases expression of a transcript or protein encoded by the endogenous gene.

The application belongs to the field of molecular biology, and discloses a SNP molecular marker related to a pig backfat thickness character and application thereof. The application screens a SNP site g.348A>G related to the pig backfat thickness character in an intron region of an ADIPOQ gene. The SNP site is located at a 348bp nucleotide downstream of a transcription start site of the ADIPOQ gene, and the polymorphism is A or G. The genotype of the mutation site is used to determine the pig backfat thickness, thereby obtaining a functional gene and a molecular genetic marker related to the pig backfat thickness. Through selection of a dominant allele of the SNP molecular marker and rapid detection of the site, a reference basis can be provided for accurate evaluation of a pig fat deposition capacity regulation related SNP molecular marker assisted breeding, and the application has important significance for pig line or breed improvement.

This specification provides isolated nucleic acids, vectors, recombinant cells, and genetically modified animals capable of expressing at least one or more mammoth genes. Recombinant cells and genetically modified animals containing one or more mammoth-specific deletions of upstream regulatory regions of identified transcription start sites are also provided.

The application discloses a Gossypium barbadense L. GbCYP72A2 gene, a coding protein thereof and application. The gene has a transcription start site at the 1st base from the 5' end, and a transcription termination codon at the 1558-1560th base. The coding frame is 1557 bases, and the gene codes 519 amino acids. The gene has 97% similarity with a homologous gene GbCYP72A1, but 3% sequence difference can endow a plant with higher resistance to verticillium wilt. The gene can enrich disease-resistant gene resources, provide an effective new tool for cotton breeding against verticillium wilt, and the overexpression of the gene can reduce the sensitivity of a plant to ABA in the germination stage. The down-regulated expression of the gene can also promote the growth of the plant.

Methods and compositions are provided for using transcription start site (TSS) profiling to identify alternate promoters that yield better transcription modulation (e.g., knockdown using CRISPRi or activation using CRISPRa). Methods can also include designing CRISPR-Cas guide RNAs to target CRISPRi or CRISPRa complexes to the identified alternative promoters, and methods can also include steps of generating such guide RNAs. Also provided are libraries of guide RNA, methods of modulating expression of a target gene. In some cases, multiple promoters for the same gene are targeted simultaneously.

The present application relates to the technical field of molecular marker, and particularly relates to a SNP molecular marker, primers and application associated with the antibody level of African swine fever virus. The SNP molecular marker is located at 22bp upstream of the transcription start site of the pig PSMC2 gene with the gene version number of NM_001245008.1, and has G / A polymorphism. The SNP molecular marker provided by the present application is closely associated with the antibody level of African swine fever virus of pigs. Compared with the pigs with the genotype of GA and AA at the site, the pigs with the genotype of GG have higher antibody level of African swine fever virus in serum. The SNP molecular marker can be applied to identify high-quality pigs resistant to African swine fever virus, so as to improve the breeding efficiency of breeding pigs and effectively improve the economic benefits of feeding pigs.

The purpose of the present invention is to further improve the efficiency of translation by means of a technique for translating a protein using RNA that encodes a protein and a capped polynucleotide that can bind thereto by complementary base pairing, and to improve the efficiency of capping for producing a 5 '-capped polynucleotide. This problem is solved by causing a 5 '-capped polynucleotide and / or the single-stranded RNA to have a translation efficiency promoting structure, and inserting a base into the promoter such that the base sequence of the polynucleotide of the cap analogue and the base sequence upstream from the transcription start point of the promoter complement each other in a specific positional relationship.

The present disclosure relates to promoters engineered to increase responsiveness to C2 carbon sources. Engineered promoters comprise at least one external carbon source responsive element (CSRE) located upstream and proximal to a transcription initiation site of a gene operably associated therewith. The engineered promoters can be used in heterologous nucleic acid molecules, vectors, or expression cassettes to promote expression of genes in microorganisms. The disclosure also relates to methods for producing the engineered promoters and methods for expressing genes in recombinant microbial host cells using the engineered promoters.

1. An RNA molecule comprising in the 5'3' direction, a 5'-terminal transcription start site, a 5' untranslated region (5'UTR), a Kozak sequence, an open reading frame (ORF) comprising a coding region, and a 3' untranslated region (3'UTR), wherein a) the 5'UTR comprises a transcript of the 5'UTR of the human alpha-globin nucleic acid sequence SEQ ID NO:1, a fragment or variant thereof which is at least 90% identical to SEQ ID NO:1; and b) the 3'UTR comprises a transcript of: i) the human Glucose 6-phosphate isomerase 1 (GPI1) nucleic acid sequence SEQ ID NO:19, or a fragment of SEQ ID NO:19 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:19 which is at least 90% identical to SEQ ID NO:19; or ii) the human nascent polypeptide associated complex subunit alpha (NACA) nucleic acid sequence SEQ ID NO:21, or a fragment of SEQ ID NO:21 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:21 which is at least 90% identical to SEQ ID NO:21; or iii) the human magnesium transporter (MRS2) nucleic acid sequence SEQ ID NO:23, or a fragment of SEQ ID NO:23 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:23 which is at least 90% identical to SEQ ID NO:23; or iv) a combination of any two or more of i), ii), or iii).