14 results about "Transcription start" patented technology

The application belongs to the technical field of biological gene engineering, and particularly relates to a barley HvCslf6 promoter element and application thereof in regulating the content of barley grain beta-glucan, wherein the barley HvCslf6 promoter element is located at 295bp-300bp upstream of a transcription start site of a barley HvCslf6 gene; the nucleotide sequence of the HvCslf6 promoter element is CCGTTG, and the HvCslf6 promoter element belongs to a CCAAT-box element; the gene number of the HvCslf6 gene in BARLEX Morex v3 Gene Models is HORVU.MOREX.r3.7HG0698110. The application finds a key element which can affect the content of beta-glucan in barley grains in the upstream promoter region of a main effect gene HvCslf6 of barley beta-glucan synthesis by using a gene editing technology, and thus provides a method capable of preparing transgenic plants with changed content of beta-glucan in barley grains, and provides a new means and tool for detecting and regulating the content of beta-glucan in barley grains.

The integration procedure provides different elements that require in vitro amplification of HSPCs, preferably for about 48 hours, in the presence of hematopoietic cytokines as well as UM171 and stem cell regenerative factor 1, and the introduction of i) a system (e.g., a CRISPR / Cas9 system) that generates DNA double-strand breaks (DSBs) upstream of the transcription start site of the target gene, and ii) the introduction of a donor matrix comprising cDNA with homologous arms on both sides (left [LHA] and right [RHA]).

The invention relates to a cancer non-invasive early screening method and system based on sequencing coverage depth and fragment size characteristics near a cfDNA transcription start site.

The application provides a non-coding mutation-induced risk gene expression up-regulation and down-regulation prediction method and system, comprising the following steps: step 1, obtaining a data set and performing pretreatment; step 2, information input standardization between non-coding mutations and gene transcription start points TSS; step 3, non-coding mutation information input standardization; step 4, sequence length alignment and representation learning framework; and step 5, embedded feature merging and prediction output. The application is matched with chromatin accessibility sequencing data to migrate to any tissue or cell type, make accurate and reliable prediction, and utilize an attention mechanism to endow the model with interpretability.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.

This invention belongs to the field of plant molecular biology and genetic breeding technology, specifically relating to a molecular marker related to the malic acid content of apple fruit and its application. The molecular marker is... MdNAC029 The single nucleotide polymorphism site in the gene promoter region, this site is located MdNAC029 The nucleotide sequence of the gene promoter region, starting from the 2693rd base at the 5' end, corresponds to... MdNAC029 At a position 442 bp upstream of the transcription start codon, the polymorphism is either T or C, with the C / C genotype indicating high malic acid content and the T / T genotype indicating low malic acid content. This invention, through molecular biology experiments, confirms that the T allele is the binding site for the transcriptional repressor MdMYC2, and the C allele eliminates the binding of MdMYC2, thereby relieving transcriptional repression and promoting malic acid accumulation. This provides a new molecular marker for the genetic improvement of apple fruit acidity traits, accelerating the breeding process of superior varieties.

The application discloses a method for eRNA transcription identification, regulation target prediction and function annotation based on high-throughput transcriptome sequencing data, and is characterized in that the method comprises the following steps: identifying part of non-coding RNA in which a transcription start site is located in an enhancer region as eRNA; obtaining eRNA-related protein coding genes which simultaneously exist in an eRNA-protein coding gene co-expression network and an eRNA-centered regulation network, constructing an eRNA-protein coding gene relationship network, and extracting protein coding genes which are directly connected or closely connected to the eRNA, so as to predict potential regulation targets of the eRNA; and finally, performing function enrichment analysis on the potential regulation targets of the eRNA, so as to obtain the results of eRNA function annotation, and the method has the advantages of wider application range, and can be applied to all eRNAs and more accurately obtain the action forms between the eRNA and the protein coding gene.

Provided herein are isolated nucleic acids, vectors, recombinant cells, and transgenic animal tremor longimanus capable of expressing at least one or more tremor longimanus genes. Also provided are recombinant cells and transgenic animals comprising one or more abrupt hair gene-specific deletions in the regulatory region upstream of the identified transcription initiation site.

A method for increasing gene expression and improving herbicide resistance in plants. Specifically, the method involves performing modifications at upstream and downstream regions of a transcription start site of a gene of interest by means of a genome editing technique, so as to modify a non-coding region of the gene, thereby increasing the expression level of an endogenous polynucleotide or the gene of interest. Furthermore, the method is used to modify and regulate an HPPD gene and / or HIS1 gene associated with herbicide resistance in the plants, thereby improving herbicide resistance in the plants.

The present invention relates to synthetic enhancer elements that can be used to increase the expression of a gene of interest in any plant tissue. The disclosed synthetic enhancer elements can be used in a gene expression cassette comprising nucleic acid further comprising a promoter and a sequence to be expressed, wherein the sequence to be expressed has a transcription start site, and wherein the synthetic enhancer element is operably linked to the promoter and the sequence to be expressed. The present invention also relates to a modified plant or part thereof comprising a synthetic enhancer element, wherein the synthetic enhancer element is operably linked to an endogenous promoter in the modified plant or part thereof and an endogenous gene in the modified plant or part thereof, and wherein the synthetic enhancer element increases expression of a transcript or protein encoded by the endogenous gene.

This specification provides isolated nucleic acids, vectors, recombinant cells, and genetically modified animals capable of expressing at least one or more mammoth genes. Recombinant cells and genetically modified animals containing one or more mammoth-specific deletions of upstream regulatory regions of identified transcription start sites are also provided.

The present application relates to the technical field of molecular marker, and particularly relates to a SNP molecular marker, primers and application associated with the antibody level of African swine fever virus. The SNP molecular marker is located at 22bp upstream of the transcription start site of the pig PSMC2 gene with the gene version number of NM_001245008.1, and has G / A polymorphism. The SNP molecular marker provided by the present application is closely associated with the antibody level of African swine fever virus of pigs. Compared with the pigs with the genotype of GA and AA at the site, the pigs with the genotype of GG have higher antibody level of African swine fever virus in serum. The SNP molecular marker can be applied to identify high-quality pigs resistant to African swine fever virus, so as to improve the breeding efficiency of breeding pigs and effectively improve the economic benefits of feeding pigs.

The purpose of the present invention is to further improve the efficiency of translation by means of a technique for translating a protein using RNA that encodes a protein and a capped polynucleotide that can bind thereto by complementary base pairing, and to improve the efficiency of capping for producing a 5 '-capped polynucleotide. This problem is solved by causing a 5 '-capped polynucleotide and / or the single-stranded RNA to have a translation efficiency promoting structure, and inserting a base into the promoter such that the base sequence of the polynucleotide of the cap analogue and the base sequence upstream from the transcription start point of the promoter complement each other in a specific positional relationship.

1. An RNA molecule comprising in the 5'3' direction, a 5'-terminal transcription start site, a 5' untranslated region (5'UTR), a Kozak sequence, an open reading frame (ORF) comprising a coding region, and a 3' untranslated region (3'UTR), wherein a) the 5'UTR comprises a transcript of the 5'UTR of the human alpha-globin nucleic acid sequence SEQ ID NO:1, a fragment or variant thereof which is at least 90% identical to SEQ ID NO:1; and b) the 3'UTR comprises a transcript of: i) the human Glucose 6-phosphate isomerase 1 (GPI1) nucleic acid sequence SEQ ID NO:19, or a fragment of SEQ ID NO:19 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:19 which is at least 90% identical to SEQ ID NO:19; or ii) the human nascent polypeptide associated complex subunit alpha (NACA) nucleic acid sequence SEQ ID NO:21, or a fragment of SEQ ID NO:21 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:21 which is at least 90% identical to SEQ ID NO:21; or iii) the human magnesium transporter (MRS2) nucleic acid sequence SEQ ID NO:23, or a fragment of SEQ ID NO:23 comprising at least 50% of the full-length sequence, or a variant of SEQ ID NO:23 which is at least 90% identical to SEQ ID NO:23; or iv) a combination of any two or more of i), ii), or iii).