The invention belongs to the field of
genetic engineering, and in particular relates to a salt
stress inducible promoter as well as primers, an
expression vector and application thereof. A
promoter fragment is cloned, a vector of the
promoter fragment is constructed,
arabidopsis genetic transformation is performed, an
arabidopsis resistant material is screened and detected, GUS
staining is carriedout, and RT-qPCR is used for detecting the expression situation of a GUS
gene. The results show that the promoter fragment provided by the invention can drive the expression of the GUS
gene and can be expressed in roots, stems, leaves, flowers and other parts; under the condition of
abiotic stress induction, the activity and driving efficiency of the promoter are also higher than those under thenormal conditions; the qPCR results also show the similar results, so that the promoter provided by the invention can improve the salt tolerance of transgenic crops; therefore, the salt
stress inducible promoter has a wide application value.