10 results about "Sitobion avenae" patented technology

The invention relates to application of limonin as a pesticide synergistic aid in prevention and control of corn pests, which utilizes the characteristic that low-concentration limonin has a remarkable synergistic effect on chemical pesticides, 0.5-4g / mL limonin, 5% chlorantraniliprole and 25% thiamethoxam are mixed for use, so that the lethal medium concentration (LC50) of the limonin to corn borers, corn aphids and rhopalosiphum padi can be remarkably reduced; the insecticidal effect of chlorantraniliprole on corn borer and the insecticidal effect of thiamethoxam on aphid are improved. A field pesticide effect test also shows that the field prevention and control effect of the pesticide on Lepidoptera pests such as ostrinia nubilalis is remarkably improved by mixing 4 g / mL of limonin and 5% of chlorantraniliprole, the actual field dosage of chemical pesticides is reduced, the purposes of pesticide reduction and synergism are achieved, and a positive effect is achieved on comprehensive prevention and control of the field pests.

The application discloses an RNAi pesticide for preventing and treating Sitobion miscanthi, which comprises a double-stranded RNA molecule dsRNA targeting a Sitobion miscanthi gene Sacdc42 with a nucleotide sequence of SEQ ID NO:1. The RNAi pesticide can effectively reduce the harm of Sitobion miscanthi to the growth of wheat and effectively improve the thousand-grain weight of the wheat.

The invention belongs to the technical field of pesticides, and particularly relates to a triazole compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein X represents CR11 or N; x2 represents CR2 or N; w represents O or S; r1, R2, R3, R4, R5, R6, R7, R9, and R11 each independently represent hydrogen, halogen, alkyl, or the like; r8 represents hydrogen, an alkyl group, an alkenyl group, an alkynyl group, or the like; r10 represents hydrogen, an alkyl group, an alkenyl group, an alkynyl group, or the like; the compound has excellent prevention and treatment effects on brown planthopper, rhopalosiphum padi, laodelphax striatellus, sogatella furcifera, green peach aphid, peanut aphid and the like.

This invention belongs to the field of plant protection, specifically relating to the application of eugenol in the control of wheat aphid. The research of this invention demonstrates the repellent, contact-killing, and fumigation effects of eugenol on the agricultural pest wheat aphid. The effects of eugenol on the growth and development of the agricultural pest wheat aphid were assessed using sex life table software, evaluating key parameters of wheat aphid growth and development and population changes at sublethal concentrations. The results showed that eugenol treatment can adversely affect the growth and development of wheat aphids, such as reducing survival and reproductive rates, and decreasing population size. Furthermore, eugenol has a certain synergistic effect with commonly used insecticides. Therefore, this invention has application value in the control of wheat aphids.

The invention discloses a wheat aphid resistance gene ARG1 and application thereof. The nucleotide sequence of the wheat aphid resistance gene ARG1 is shown as SEQ ID NO.3, and the amino acid sequence coded by the wheat aphid resistance gene ARG1 is shown as SEQ ID NO.4. The invention further discloses a method for preparing the wheat aphid resistance gene ARG1. The wheat aphid-resistant gene ARG1 can be used for identifying or breeding wheat aphid-resistant germplasm resources, and the breeding process of wheat aphid-resistant varieties is accelerated.

The application discloses a kind of dsRNA of Sitobion avenae CYP4C1 gene and application thereof, relate to the technical field of agricultural pest control, the cDNA of Sitobion avenae CYP4C1 gene is cloned in the application, its nucleotide sequence is as shown in SEQ ID NO.1;And the dsRNA of the gene is prepared, nucleotide sequence is as shown in SEQ ID NO.2;The preparation method of the dsRNA includes total RNA extraction, cDNA synthesis, target gene cloning, T7 promoter fragment amplification and in vitro transcription purification steps;The dsRNA of Sitobion avenae CYP4C1 gene and application thereof, by feeding method, the dsRNA is introduced into the 3rd instar nymph of Sitobion avenae, and after feeding 48h, CYP4C1 gene silencing efficiency reaches 58%, can significantly reduce the survival rate and the amount of aphid of Sitobion avenae on imidacloprid seed dressing wheat seedling, target strong, prevention and control effect is remarkable, no chemical residue.

The application relates to application of limonin as a pesticide synergist in corn pest control, and the characteristics that low-concentration limonin has a significant synergistic effect on chemical pesticides. 0.5-4 mu g / mL limonin is mixed with 5% chlorantraniliprole and 25% thiamethoxam for use, the lethal concentration (LC50) of the mixture to corn borer, corn aphid and Sitobion avenae can be significantly reduced, and the insecticidal effect of chlorantraniliprole on corn borer and thiamethoxam on aphids is improved. Field efficacy tests show that 4 mu g / mL limonin mixed with 5% chlorantraniliprole not only significantly improves the field control effect of the pesticide on lepidopterous pests such as corn borer, but also reduces the actual field use amount of the chemical pesticide, that is, the purpose of reducing the pesticide and improving the synergistic effect is achieved, and the method has a positive effect on comprehensive field pest control.

The invention relates to a suitable citrobacter strain and application thereof. The invention relates to citrobacter suitabilis strain J35, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the citrobacter suitabilis strain J35 is CGMCC No.36708, and the citrobacter suitabilis strain J35 has insecticidal activity on Sitobion avenae, Rhopalosiphum padi, Amyzus persicae, Lygocoris lucorum, Bemisia tabaci, Plutella xylostella and Spodoptera litura. The citrobacter suitabilis strain J35 has the advantages that the strain J35 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the citrobacter suitabilis strain J35 is CGMCC

The invention belongs to the technical field of pesticides, and particularly relates to a fused heterocyclic compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, Q1 and Q2 independently represent N or CR8 respectively, and Q1 and Q2 are not N at the same time; q3 represents O or NR9; q4 represents O, S or CR10R11; r5 represents-S (O) nR21; r1, R2, R3, R4, R6, R7, R8, R10, and R11 each independently represent hydrogen, halogen, an alkyl group, or the like; r9 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group, or the like; the compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, brown planthopper, rhopalosiphum padi and the like.

This invention belongs to the field of biotechnology, specifically relating to the design and application of an RNAi target gene and its dsRNA for controlling the wheat aphid. The invention involves sequencing, annotation, and verification to obtain the wheat aphid tyrosine hydroxylase gene, whose nucleotide sequence is SEQ ID No: 1. Using the wheat aphid tyrosine hydroxylase gene as a target gene, dsRNA was designed, including the design of primers with a 5' end T7 promoter sequence, resulting in the nucleotide sequence of the tyrosine hydroxylase dsRNA, which is SEQ ID No: 4. This invention uses the wheat aphid tyrosine hydroxylase gene as a target gene and designs corresponding wheat aphid tyrosine hydroxylase dsRNA, both of which are applied to control the wheat aphid, leading to inhibited growth and development and a lethal effect.