2 results about "Sitobion avenae" patented technology

This invention belongs to the field of plant protection, specifically relating to the application of eugenol in the control of wheat aphid. The research of this invention demonstrates the repellent, contact-killing, and fumigation effects of eugenol on the agricultural pest wheat aphid. The effects of eugenol on the growth and development of the agricultural pest wheat aphid were assessed using sex life table software, evaluating key parameters of wheat aphid growth and development and population changes at sublethal concentrations. The results showed that eugenol treatment can adversely affect the growth and development of wheat aphids, such as reducing survival and reproductive rates, and decreasing population size. Furthermore, eugenol has a certain synergistic effect with commonly used insecticides. Therefore, this invention has application value in the control of wheat aphids.

The application discloses a kind of dsRNA of Sitobion avenae CYP4C1 gene and application thereof, relate to the technical field of agricultural pest control, the cDNA of Sitobion avenae CYP4C1 gene is cloned in the application, its nucleotide sequence is as shown in SEQ ID NO.1;And the dsRNA of the gene is prepared, nucleotide sequence is as shown in SEQ ID NO.2;The preparation method of the dsRNA includes total RNA extraction, cDNA synthesis, target gene cloning, T7 promoter fragment amplification and in vitro transcription purification steps;The dsRNA of Sitobion avenae CYP4C1 gene and application thereof, by feeding method, the dsRNA is introduced into the 3rd instar nymph of Sitobion avenae, and after feeding 48h, CYP4C1 gene silencing efficiency reaches 58%, can significantly reduce the survival rate and the amount of aphid of Sitobion avenae on imidacloprid seed dressing wheat seedling, target strong, prevention and control effect is remarkable, no chemical residue.