41 results about "Sitobion avenae" patented technology

The invention discloses a dsRNA (double-strand ribonucleic acid) and an application of combinations thereof to control aphid damage. The dsRNA consists of (1) a nucleotide shown as a sequence 4 in a sequence table and a nucleotide shown as a reversely complementary sequence of the sequence 4; (2) a nucleotide shown in a sequence 5 in the sequence table and a nucleotide shown as a reversely complementary sequence of the sequence 5; or (3) a dsRNA shown by (1) and a dsRNA shown by (2). Experiments show that effects of inhibiting the growth and development of sitobion avenae and causing the death of the sitobion avenae are achieved by feeding the dsRNA for growth and development related genes 8273 and 22544 cDNA (complementary deoxyribonucleic acid) of the sitobion avenae in vitro and silencing the growth and development related genes 8273 and 22544 of the sitobion avenae by utilizing an RNAi (RNA interference) technology.

The invention discloses a dsRNA (Double-stranded Ribonucleic Acid) of a sitobion avenae symbiotic bacteria gene and application thereof in reducing the sitobion avenae survival rate. The dsRNA provided by the invention is a double-stranded RNA consisting of the nucleotide shown as a sequence 1 in a sequence list and the nucleotide shown as a reverse complementary sequence. According to the dsRNA provided by the invention, the Pseudomonas putida 29698 gene of the symbiotic bacteria in a sitobion avenae body is silenced by an in-vitro dsRNA feeding method through the utilization of a RNAi (RNA interfere) technology, lethal effect is produced on the sitobion avenae, and the dead rate of the sitobion avenae is gradually increased with the prolongation of the feeding time. The real-time fluorescent quantitative polymerase chain reaction study of the Pseudomonas putida 29698 gene of the symbiotic bacteria in the fed avenae body shows that the expression of the 29698 gene is obviously inhibited and that a conservative sequence of the Pseudomonas putida 29698 gene of the symbiotic bacteria in the avenae body can be applied in a study improving the wheat avenae resistance through a plant mediating RNAi technology.

The invention relates to siRNA based on Sitobion avenae SaveOrco gene design and application thereof.Transcription of a SaveOrco gene is interfered through feeding of siOrco7.Real-time fluorescent quantitative polymerase chain reaction (PCR) research shows that the expression of the SaveOrco gene is obviously restrained.Olfactory behavior reaction detection results show that the responding rates of silenced aphids to z-3-hexenol, methyl salicylate and EBF are obviously reduced under SaveOroco expression.An EBF inducing experiment for sitobion avenae winged morph differentiation is developed, siRNA processed aphids stop inducing of EBF, normal chemical communication of the aphids is interfered, and stress inducible wing bud generation is also stopped.In this way, a conserved sequence of the Orco gene can be applied to an RNAi technology mediated by siRNA, and the aphids can be effectively restrained from rapidly diffusing to be harmful and are prevented and treated.

The invention discloses a DNA molecule for expressing hairpin RNA for inhibiting a macrosiphum avenae lipoprotein lipase and applications thereof. The structure of the DNA molecule provided by the invention is SEQ positive direction and -X-SEQ reverse direction, and the sequence of the SEQ positive direction is 21st-563rd position of a sequence 1 in a sequence table; the sequence if the SEQ reverse direction is reversely complemented to the sequence of the SEQ positive direction; and X is an interval sequence between the SEQ positive direction and the SEQ reverse direction, and in the sequence, X does not complement to the SEQ positive direction and the SEQ reverse direction. Proved by an experiment, the DNA molecule is converted to be wheat, to obtain transgene lines for carrying out a aphid feeding test, and after a macrosiphum avenae eats transgene leaves, a lipoprotein lipase gene expression level and reproductive rate decrease. Thus, the DNA molecule provided by the invention has an important meaning for controlling insects in agricultural production.

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and / or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

The invention relates to siRNA designed based on the conservative olfactory receptor Orco gene of sitobion avenae and an application thereof. In the invention, siOrco8 is fed to interfere with the transcription of the SaveOrco gene. Real-time fluorescent quantitative PCR study indicates that the expression of the SaveOrco gene is obviously inhibited. The olfactory behavior reaction detection result shows that the responsivity of aphids with expression loss of SaveOrco to cis-3-hexenol, methyl salicylate and EBF is remarkably reduced. In an experiment of EBF inducing wing dimorphism of sitobion avenae, the aphids treated with siRNA stop induction of EBF, normal chemical communication of the aphids is disturbed, and the stress-inducible wing bud generation is also stopped. Therefore, the conservative sequence of the Orco gene can be used for effectively preventing aphids from quickly spreading and doing harm through the siRNA-mediated RNAi technology so as to control aphids.

The invention discloses an aphid biological control method with barley and Sitobion avenae as storage carriers. The method comprises the steps that A. barley reproduction is carried out, wherein after barley seeds are subjected to sterilization and deinsectization, a culturing medium is used for barley reproduction; B. Sitobion avenae reproduction is carried out, wherein Sitobion avenae is collected and sent into barley for culturing reproduction; C. aphidiidae inoculation is carried out, the aphidiidae is collected and put into barley where aphid breeding is completed for culturing; D. field releasing is carried out, wherein barley with aphidiidae are placed between lines of crops; and E. carrier plant maintaining and replacing are carried out, wherein timing moisture supplementing and in-due-time barley replacing are carried out. The aphid biological control method with the barley and the Sitobion avenae as the storage carriers is easy to operate, low in cost and obvious in effect.

The invention discloses an application of dsRNA of an ecdysone receptor (EcR) related gene USP to control of damage caused by aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that dsRNA of cDNA of the EcR related gene of sitobion avenae is obtained; expression of the gene USP in sitobion avenae can be inhibited, impaired growth and development of wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention discloses a carboxylesterase gene dsRNA and applications thereof in prevention and treatment of sitobion avenae. The dsRNA is a double-chain RNA consisting of nucleotide shown in a sequence 1 in a sequence table and nucleotide shown in a reverse complementary sequence of the sequence 1. Experiments proves that carboxylesterase genes in sitobion avenae are reduced to different degrees after the sitobion avenae is fed with carboxylesterase gene dsRNA, which means that dsRNA taken in by a lancet enters the cells of the sitobion avenae to generate RNAi cascade reaction and the expression of a target gene is interfered. In a morphological view, the growth state of the sitobion avenae is changed, and the ecdysis index of the sitobion avenae fed with the carboxylesterase gene dsRNA is significantly reduced in comparison with a control. Therefore, carboxylesterase gene dsRNA can be used in prevention and control of sitobion avenae. The invention lays a foundation for construction of new germplasm of wheat by using plant-mediated RNAi technology in the future.

The invention relates to siRNA based on Sitobion avenae SaveOrco gene design and application thereof.Transcription of a SaveOrco gene is interfered through feeding of siOrco7.Real-time fluorescent quantitative polymerase chain reaction (PCR) research shows that the expression of the SaveOrco gene is obviously restrained.Olfactory behavior reaction detection results show that the responding rates of silenced aphids to z-3-hexenol, methyl salicylate and EBF are obviously reduced under SaveOroco expression.An EBF inducing experiment for sitobion avenae winged morph differentiation is developed, siRNA processed aphids stop inducing of EBF, normal chemical communication of the aphids is interfered, and stress inducible wing bud generation is also stopped.In this way, a conserved sequence of the Orco gene can be applied to an RNAi technology mediated by siRNA, and the aphids can be effectively restrained from rapidly diffusing to be harmful and are prevented and treated.

The invention relates to a method for raising aphid-eating gall midges by using a mixed population of various aphids on barley. In the method, barley seedlings are selected as host plants, and three kinds of aphids mixed populations of corn aphid, corn aphid and wheat long tube aphid are used. As an intermediate host, the aphid-loaded wheat seedlings of the three aphid mixed populations were used to breed the aphid gall midge, so as to improve the large-scale reproduction efficiency of the aphid midge. The invention uses barley as a host plant, and has the characteristics of short seedling raising period, high planting density, large aphid loading capacity and the like. Simultaneously, the present invention also adopts the method of raising aphid-eating gall midges in mixed populations of three different aphids (maize aphid, cereal aphid and wheat long tube aphid), which further improves the large-scale reproduction efficiency. In the case that the prey aphid load on the host plant has an upper limit, increasing the number of eggs laid by aphid gall midges by increasing the species of aphids has opened up a new way to improve the efficiency of large-scale reproduction.

The invention discloses an application of dsRNA of an ecdysone receptor (EcR) related gene to control of damage caused by aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that dsRNA of cDNA of the EcR related gene of sitobion avenae is obtained; expression of the EcR gene in sitobion avenae can be inhibited, impaired growth and development of wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention relates to the development and application of a bacteria and pest killing compound agent for wheat. The compound agent mainly consists of tebuconazole, triazolone and imidacloprid and can be prepared into missible oil, missible oil, wettable powder and the like. Demonstrated by biological test, the compound agent has obvious synergistic function for wheat stripe rust and erysiphe graminis and can also prevent and kill aphid. According to field experiments, the compound agent can achieve 90 percent of effect in preventing wheat leaf diseases, more than 90 percent of effect in preventing the erysiphe graminis, no less than 98 percent of effect in preventing and controlling wheat sitobion avenae. The compound agent has the functions of killing bacteria and pests, is conveniently used, saves work time, reduces cost and environment pollution, and actively responds to the national policy of energy conservation and emission reduction. Meanwhile, the compound agent has simple process, is easy to produce, can be directly transformed into a production capability and can obtain economic and social benefits as soon as possible.

The invention discloses a partial sequence, a cloning method and an application of an internal reference gene of the elongated tube aphid, and belongs to the field of molecular biology, and specifically relates to internal reference genes SA‑HEL, SA‑RPL14, SA‑SA‑RPL11, SA -28S, its nucleotide sequence is shown in SEQ ID No.1, SEQ ID No.6, SEQ ID No.11, SEQ ID No.16 respectively. The nucleotide sequence of the internal reference gene of the aphid aphid obtained by the present invention can be used as an internal reference gene for the future research on the functional gene of the aphid or gene expression in different developmental stages, the gene expression in the body of the aphid and the aphid, or as an internal reference gene for different aphids. Density treatment, or as a solution of the pheromone E‑β‑farnesene, or as a useful method for research under insecticide or antibiotic treatment.