41 results about "Sitobion avenae" patented technology

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and / or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

The invention discloses an aphid biological control method with barley and Sitobion avenae as storage carriers. The method comprises the steps that A. barley reproduction is carried out, wherein after barley seeds are subjected to sterilization and deinsectization, a culturing medium is used for barley reproduction; B. Sitobion avenae reproduction is carried out, wherein Sitobion avenae is collected and sent into barley for culturing reproduction; C. aphidiidae inoculation is carried out, the aphidiidae is collected and put into barley where aphid breeding is completed for culturing; D. field releasing is carried out, wherein barley with aphidiidae are placed between lines of crops; and E. carrier plant maintaining and replacing are carried out, wherein timing moisture supplementing and in-due-time barley replacing are carried out. The aphid biological control method with the barley and the Sitobion avenae as the storage carriers is easy to operate, low in cost and obvious in effect.

The invention relates to a method for raising aphid-eating gall midges by using a mixed population of various aphids on barley. In the method, barley seedlings are selected as host plants, and three kinds of aphids mixed populations of corn aphid, corn aphid and wheat long tube aphid are used. As an intermediate host, the aphid-loaded wheat seedlings of the three aphid mixed populations were used to breed the aphid gall midge, so as to improve the large-scale reproduction efficiency of the aphid midge. The invention uses barley as a host plant, and has the characteristics of short seedling raising period, high planting density, large aphid loading capacity and the like. Simultaneously, the present invention also adopts the method of raising aphid-eating gall midges in mixed populations of three different aphids (maize aphid, cereal aphid and wheat long tube aphid), which further improves the large-scale reproduction efficiency. In the case that the prey aphid load on the host plant has an upper limit, increasing the number of eggs laid by aphid gall midges by increasing the species of aphids has opened up a new way to improve the efficiency of large-scale reproduction.

The invention discloses a partial sequence, a cloning method and an application of an internal reference gene of the elongated tube aphid, and belongs to the field of molecular biology, and specifically relates to internal reference genes SA‑HEL, SA‑RPL14, SA‑SA‑RPL11, SA -28S, its nucleotide sequence is shown in SEQ ID No.1, SEQ ID No.6, SEQ ID No.11, SEQ ID No.16 respectively. The nucleotide sequence of the internal reference gene of the aphid aphid obtained by the present invention can be used as an internal reference gene for the future research on the functional gene of the aphid or gene expression in different developmental stages, the gene expression in the body of the aphid and the aphid, or as an internal reference gene for different aphids. Density treatment, or as a solution of the pheromone E‑β‑farnesene, or as a useful method for research under insecticide or antibiotic treatment.