13results about How to "Reduce enzyme activity" patented technology

The invention discloses application of a TaHSFA6e gene in regulation and control of salt stress tolerance of plants, and belongs to the technical field of gene engineering. The nucleotide sequence of the TaHSFA6e gene disclosed by the invention is as shown in at least one of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 8 and SEQ ID NO: 12. According to the invention, four TaHSFA6e family genes are cloned from wheat, and the expression quantity of the four genes in leaves and roots after wheat salt stress is obviously improved. After the four genes are mutated, the salt tolerance of the mutated wheat is obviously reduced. In conclusion, the four TaHSFA6e family genes are related to the salt tolerance of the wheat, can be used for regulating and controlling the salt tolerance of the wheat and enriching wheat germplasm resources, and have important application value in breeding of the salt-tolerant wheat.

The invention relates to the technical field of microorganisms and the field of food, in particular to lactobacillus plantarum for inhibiting brown stain of soybean paste and application of lactobacillus plantarum. The lactobacillus plantarum is screened from traditional naturally-fermented peasant soybean paste in northeast China, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 30, 2025, and the preservation number is CGMCC No.35048. The lactobacillus plantarum has an obvious browning inhibition effect and can be widely applied to fermented food production.

The invention provides an MOF-on-MOF composite material, an aptamer probe as well as a preparation method and application of the aptamer probe. The MOF-on-MOF composite material comprises an HKUST-1 material, the surface of the HKUST-1 material is coated with a Cu-BDC nanosheet; the synthesized HKUST-1 (at) Cu-BDC / Apt aptamer probe can specifically recognize AFB1, the aptamer probe can directly capture the AFB1 and adsorb the AFB1 to active sites on the surface of a material to reduce the nano-enzyme activity of the AFB1, so that quantitative colorimetric detection of the AFB1 is realized, and the aptamer probe has low detection limit, wide detection range, good selectivity and anti-interference performance on the AFB1 and can be used for detecting the AFB1. The AFB1 in a food sample can be rapidly, sensitively and selectively detected. In addition, the composite material has an adsorption effect and high catalytic activity on fungaltoxin, and AFB1 can be efficiently removed through the adsorption effect and the catalytic action.

The present application relates to a kind of passion fruit explant brown prevention tissue culture medium and its tissue culture method, it is related to passion fruit tissue culture technical field.The present application provides a kind of passion fruit explant brown prevention tissue culture medium, the tissue culture medium includes the following components:MS culture medium, passion fruit juice, 6-BA, IBA, sucrose, agar.The present application is improved by culture medium, explant low-temperature treatment and explant secondary disinfection, and the pollution rate of explant, browning rate is reduced, improves the survival rate of passion fruit explant in the process of tissue culture, overcomes the shortcoming that browning mortality is high in the process of passion fruit tissue culture, improves the tissue culture efficiency of passion fruit.

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

The invention discloses a catalytic system and method for catalyzing hydrolysis of halogenated organic matters on an oil-water interface by enzyme. The catalytic system comprises an organic solvent, an aqueous solution and an enzyme-polymer coupling enzyme; the enzyme-polymer coupling enzyme is a conjugate formed by covalently binding halogenated alkane dehalogenase and an amphiphilic block copolymer; in the enzyme-polymer coupled enzyme, each molecule of enzyme is connected with 2-3 molecules of the amphiphilic block copolymer. According to the method, an oil-water two-phase reaction system is constructed, and the enzyme is enriched at an oil-water interface through a conjugate synthesized by an amphiphilic block polymer and the enzyme; the accessibility of a substrate in the organic solvent is improved, enzyme inactivation caused by the organic solvent is avoided, and the hydrolytic activity of enzyme to halogenated organic matters is improved; by constructing an oil-water two-phase reaction system, an enzyme catalytic reaction product hydrogen halide can be dissolved in an aqueous solution, and reduction of enzyme catalytic activity caused by retention of halide ions in an enzyme product pocket is reduced.

The invention discloses a pretreatment method of a microorganism single-cell sequencing sample and a microorganism single-cell RNA sequencing method.The pretreatment method is carried out in a sampling tube, a sample fixing cavity and at least one independent cavity isolated from the sample fixing cavity are arranged in the sampling tube, and fixing liquid is preloaded in the sample fixing cavity or the independent cavity; a diluent is preloaded in the independent chamber, and at least one of the stationary liquid and the diluent contains a nucleic acid protective agent. The method comprises the following steps: sampling a sample and sealing a sampling tube; mixing the sample with the stationary liquid under a closed condition for fixing; and the diluent and the stationary liquid are mixed in the sampling tube before, at the same time or after fixation, so that the diluted stationary liquid and the sample coexist in the sampling tube to be fixed and stored, or the stationary liquid is diluted in the sampling tube after fixation is completed and then stored. The fixing and storing processes are both completed in the closed sampling tube.

The application belongs to the technical field of skin care products, and specifically discloses a soothing and repairing composition containing a bionic sebum component and a preparation method thereof. The composition is composed of an oil phase and an aqueous phase. The oil phase comprises a bionic sebum, a farnesol-mesyl glyceride conjugate, and an enzymatic oat anthranilamide-phytosphingosine ion pair. The aqueous phase comprises deionized water, a humectant, and a crocin-hydrogenated lecithin dispersion. The application induces the liquid crystal reconstruction of the bionic sebum by sacrificing a template, so that a plurality of modified active substances form a gradient distribution from a hydrophobic core to a hydration layer in a lamellar structure, and are coupled with the skin carboxylic acid esterase activity to realize cascade release, thereby solving the problems of compatibility conflict, spatial positioning disorder and passive release of multiple active substances.

The invention relates to a biological oxygen therapy and antioxidation dual-strategy soluble microneedle as well as a preparation method and application thereof, and belongs to the technical field of medicines. The microneedle is an integrated soluble microneedle and comprises a matrix formed by a water-soluble polymer and a compound which is uniformly dispersed in the matrix and formed by microalgae and metal organic framework nano-enzyme, the compound formed by the microalgae and the metal organic framework nano-enzyme is a compound of a'core-shell 'structure, the metal organic framework nano-enzyme serving as the shell is wrapped on the surface of the microalgae serving as the core. The microneedle disclosed by the invention has the capability of rapidly resisting oxidation in situ and continuously and deeply conveying oxygen, is widely applied to preparation of medicine design or preparations for treating tumors, promoting wound healing, regulating an inflammation microenvironment, treating skin burns and scalds and removing scars, and is good in patient applicability; and the preparation process is green, environment-friendly, simple, rapid, mild in condition and suitable for large-scale production.

The utility model relates to the technical field of tea processing, and discloses a tea withering device for tea processing, which comprises a bearing plate, a processing box is fixedly connected to the middle of the top of the bearing plate, a barometer is arranged on one side of the top of the front side of the processing box, and an air outlet pipe penetrates through and is fixedly connected to one side of the bottom of the front side of the processing box. A vacuum pump is fixedly connected to the outer wall of the air outlet pipe, an air inlet pipe penetrates through and is fixedly connected to the other side of the front side of the processing box, a gas valve is fixedly connected to the outer wall of the air inlet pipe, a telescopic water pipe is slidably connected to the inner wall of the gas valve, and a cleaning assembly is arranged on one side of the telescopic water pipe. According to the tea withering device, the oxygen content and pressure of the environment can be reduced through linkage among the air inlet pipe, the air outlet pipe, the vacuum pump, the gas valve and the barometer, so that the withering process of tea can be accelerated, the tea can absorb moisture and be oxidized more uniformly, and the fragrance and taste of the tea are improved.

The invention discloses a method for preparing a novel corn superoxide dismutase multienzyme, and relates to the technical field of superoxide dismutase multienzyme preparation. The invention includes steps such as material preparation, germination, pulping, concentration, and drying. The invention selects corn from Liaoning, where the corn is produced, and the content of complex enzymes is high; a fully automatic seed cleaning, soaking, and germination device is used, and the processing capacity is large, 2t can be germinated each time, labor intensity is reduced, and the buds are evenly grown, and the content of complex enzymes is higher than that of ordinary germination methods, and large-scale industrial production can be achieved; a low-temperature vacuum concentrator is used to remove moisture at 22-25°C and concentrate, and at this temperature, the yield of complex enzyme activity is higher and the concentration multiple is higher. Experiments show that when the product of the present invention is stored at 45°C. The enzyme activity is not reduced within 90 days, and when the product of the present invention is stored at room temperature, the enzyme activity is not reduced within 48 months.

The invention belongs to the technical field of fruit and vegetable drying, and particularly relates to an apple slice drying method based on a plasma technology. The drying method comprises the following steps: performing plasma pretreatment on fresh apple slices to obtain pretreated apple slices; the time of the plasma pretreatment is 20 to 180 seconds; and performing hot air drying on the pretreated apple slices to obtain dried apple slices. According to the method, the fresh apple slices are firstly subjected to plasma pretreatment and then are dried, the etching effect of plasma can promote internal moisture of the apple slices to migrate to the surfaces, moisture evaporation is accelerated, and the drying time of the apple slices is shortened. According to the method, the drying efficiency of the apple slices can be remarkably improved, the energy consumption is reduced, the color, the shape, the rehydration capacity and the sensory quality of the dried apple slices are improved, the technical problems that in the prior art, the drying efficiency of the apple slices is low, and the product quality is reduced are solved, and a new technical approach is provided for high-value processing and utilization of the apple slices.

The invention discloses an atherosclerosis risk factor TMAO intervention key site and inhibitor screening, and belongs to the field of biological medicine. The invention provides an inhibitor aiming at the generation of TMA and / or TMAO, the inhibitor is cannabidiol, and the Cys48 site in intestinal trimethylamine lyase CutC can be targeted. By using the inhibitor, the CutC enzyme activity can be effectively reduced, and the conversion of choline to TMA is inhibited, so that the TMAO level is reduced, the promotion effect of TMAO on atherosclerosis is reduced, and the atherosclerosis risk is reduced.