50 results about "Thermostable enzymes" patented technology

The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.

The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.

A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3′-5′ exonuclease and cleaves to produce 5′-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR).

Described herein is a thermostable enzyme capable of efficient ligation of two oligomers at high temperature. The embodiments herein have led to the development of an optimized ligation step used in library preparation for sequencing reactions.

The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.

The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.

The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).

The invention provides a strain of Geobacillus thermodenitrificans as well as the screening method and the uses thereof. The strain was deposited as the number CGMCC-1228 in the China General Microbiological Culture Collection Center of the China Committee of Culture Collection for Microorganisms. The strain was obtained by primary screening, re-screening, inoculation, domestication and propagation using the bacteria in the aqueous samples from the strata of oil field as the original screening strain. The strain screened in the invention belongs to the genus Geobacillus, which can tolerate a high temperature, and has good thermostability, which can be applied to the industrial production which need the condition of thermostable enzymes, such as fermentation, etc. With its ability of growing well in the oil-reservoir environment, degrading alkanes, decreasing viscosity and increasing fluidity of crude oil, the strain is able to remarkably enhance the yield of oil and improve the transporting efficiency of oil. Additionally, with its good ability of degrading oil, it can be developed for the treatment and clearing of the material such as the oil contaminated water, etc., so it can be useful in protection of the environment. This strain also has the ability of decreasing the surface tension of substances, which can be applied to the industry of biosurfactants preparation.

The present invention relates to the field of thermostable enzymes, methods for their inactivation, and related uses, kits and reagents. In particular, the invention relates to a method for reversible inactivation of thermophilic enzymes by chemical modification under aqueous or non-aqueous conditions.

The invention relates to a method for treating a cellulosic grey fabric comprising the following steps: (a) a pretreatment step in which, in the presence of water, at a temperature of 60-100 DEG C, the fabric is contacted with a thermostable enzyme which degrades starch; and (b) an integrated desizing and scouring step in which, in the presence of water, at a temperature of 70 DEG C at the most, the fabric as obtained in step (a) is contacted with an enzyme which degrades a polymeric component of the primary cell wall of cotton and an enzyme which degrades starch. The invention also relates to the use of fabric as obtained using the method of the invention for manufacturing textile products. The invention also provides fabric manufactured using the method of the invention. The invention further relates to textile products manufactured from fabric treated using the method of the invention.

It is intended to improve a method of adding a non-thermostable enzyme to a candy base material. In the method of the invention, a non-thermostable enzyme is added to a candy base material, and the material is stirred so that a substantially uniform mixed dough-like mixture is obtained within 5 minutes from the addition to obtain the mixed dough-like mixture. Then, the mixed dough-like mixture is cooled on a cooling plate and the mixed dough-like mixture is shaped. Preferably, a main component of the candy base material is selected from the group consisting of reduced palatinose, maltitol, reduced starch syrup, and xylitol.

The present invention provides reversibly modified thermostable enzyme compositions and methods for making the same. The present invention also provides methods of using the reversibly modified thermostable enzyme compositions, as well as kits and systems comprising the reversibly modified thermostable enzymes.

The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes capable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.

In synthetic polynucleotides, the sensitivity and specificity of synthetic polynucleotides can be improved by protecting the 3' end of the oligonucleotide and then using it as a primer. Protecting the 3' end of the polynucleotide prevents non-specific chain elongation. Then, when the temperature is raised, the blocking group is removed under the action of a thermostable enzyme, thereby performing template-specific polynucleotide synthesis. The invention also provides an oligonucleotide with a blocking group at the 3' end and a thermostable enzyme capable of removing the blocking group at high temperature. The components and methods of the invention are very useful in a variety of DNA/RNA amplification and analysis techniques, including medical genetic research, diagnosis, pathogen detection, forensic science, and animal and plant genetic applications.

A synthesis method of aromatic amino acids according to one aspect of the present invention includes a process of preparing thermostable enzyme, a process of amino-transferring reaction and a process of product precipitation and enzyme recycling. The process of preparing thermostable enzyme comprises an isolation step of the gene of Thermus thermophilus aspartate aminotransferase (TtAspAT), a construction step of the TtAspAT gene in an expression vector, and a transformation step of host cells with the TtAspAT gene-harboring vector for producing a plurality of TtAspAT. The process of amino-transferring reaction catalyzed by TtAspAT, in which an amino group from an amino donor is transferred to the carbonyl group of a keto acid (the amino acceptor) through an amino-transferring reaction at the temperature between 50° C. and 80° C. for a reaction time from 30 to 90 minutes to produce aromatic amino acids. The process of product precipitation and enzyme recycling is used to recover and purify the synthesized amino acids and recycle the active aminotransferase in the supernatant of a reaction mixture.