706results about "Enzyme stabilisation" patented technology

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The disclosure provides adenosine deaminases that are capable of deaminating adenosine in DNA. The disclosure also provides fusion proteins comprising a Cas9 (e.g., a Cas9 nickase) domain and adenosine deaminases that deaminate adenosine in DNA. In some embodiments, the fusion proteins further comprise a nuclear localization sequence (NLS), and / or an inhibitor of base repair, such as, a nuclease dead inosine specific nuclease (dISN).

The invention discloses feruloyl esterase fermentation preparation and application of feruloyl esterase in polyester plastic degradation, and belongs to the field of environmental science. According to the method, feruloyl esterase FAE4 derived from Anerocephalum thermophilum is prepared through fermentation, and is compounded with polyester degrading enzymes which are different in source and are used for degrading polyester plastic oligomers, so that a compound reaction system which remarkably improves the degradation efficiency of poly (butylene adipate-co-terephthalate) or poly (butylene adipate-co-terephthalate) (PBAT) and the content proportion of a final product is obtained; good industrial prospects are realized.

The present disclosure provides an enzyme electrode that is advantageous from the viewpoint of causing a redox to occur which involves a target substance without replenishing an enzyme. An enzyme electrode (1a) according to the present disclosure is provided with: a conductive base material (10); an enzyme (21b); a metal organic structure (21a); and a fixing agent (22). The enzyme (21b) catalyzes the redox in which the target substance is involved. The fixing agent (22) fixes the enzyme (21b) and the metal organic structure (21a) to the base material (10).

The invention relates to the technical field of biosensing, in particular to a method for synergistically activating a CRISPR / Cas12a system based on a T-type structure and application of the method, the CRISPR / Cas12a system can be activated by utilizing two split DNA activation chains and crRNA to form the T-type structure, the activated CRISPR / Cas12a system has trans-cleavage activity, an FQ-ssDNA probe modified with an FAM fluorophore and a BHQ1 quenching group can be subjected to trans-cleavage, a fluorescence signal is recovered, and the fluorescence signal is recovered. Through reasonable design of sources of activation chains corresponding to different targets, detection targets can be expanded from traditional nucleic acid detection to non-nucleic acid targets such as proteins and metal ions, so that biosensing application of multiple targets is realized, in addition, design based on a T-type CRISPR / Cas12a synergistic activation strategy has great flexibility and universality, and the application prospect is wide. The method can be used together with various amplification strategies and detection platforms, and the sensitivity of the analysis method is effectively improved.

The invention relates to the field of enzyme preparations, and discloses an enzyme preparation for catalytic degradation of bioplastics and a preparation method thereof, the enzyme preparation for catalytic degradation of bioplastics comprises the following components by weight: 25-50 parts of an enzyme, 1-8 parts of a catalytic additive, 2-10 parts of a stable dispersant, and 30-50 parts of a buffer solution; the catalytic additive is prepared by using calcium bentonite as a carrier to load nano titanium dioxide; the stabilizing dispersant is prepared by introducing polyethylene glycol monoallyl ether into a sodium lignin sulfonate structure by utilizing an initiator through an aqueous solution polymerization method, and by adding the catalytic additive and the stabilizing dispersant, the enzyme preparation is endowed with good degradation efficiency on bio-based plastics and the stability of an enzyme preparation system is improved.

The invention discloses a recombinant C factor protein protective agent and application thereof. The recombinant factor C protein protective agent is prepared from the following components according to the final concentration of each component: 0.05 to 0.2 percent (w / v) of ProClin 300, 100 to 300 mM of sodium chloride, 1 to 10 mg / mL of bovine serum albumin, 5 to 20 percent (w / v) of trehalose, 10 to 50 percent (v / v) of glycerol, 0.1 to 0.5 percent (w / v) of tween-20 and 0.1 to 1 mM of ethylene glycol diethyl ether diamine tetraacetic acid with the pH value of 8.0. The invention also discloses an application of the recombinant factor C protein protective agent in refrigerated preservation of recombinant factor C protein. After the recombinant C-factor protein protective agent and the recombinant C-factor protein are mixed, the recombinant C-factor protein is prevented from being influenced by conditions such as aggregation, oxidation, freeze thawing and metal ion dependent inactivation, the stability of the recombinant C-factor protein in refrigeration storage is improved, and the activity retention rate of the recombinant C-factor protein after refrigeration storage for 12 months is greater than or equal to 90%; and the accuracy and sensitivity of endotoxin detection are maintained.

The invention provides a method, equipment, a medium and a program product for screening DES by using ANN and COSMO-RS and extracting camellia oleifera leaf polysaccharide based on the DES in combination with a two-enzyme method, and relates to the technical field of biological extraction. According to the method, on the basis of the decomposition model of the P-CL and the chemical potential information of the DESs, the relative solubility of the P-CL in the DESs is predicted by using the COSMO-RS, the pH of the DESs is predicted by using the ANN, and the most promising DES combination is screened out. A composite extraction system of double enzymes and DES extraction is provided for the first time, and the composite system is optimized by using bioinformatics methods such as COSMO-RS and ANN. The invention provides a treatment method aiming at a complex extraction environment, and the method has a wide application prospect in extraction of natural products.

Provided herein are methods of inserting a nucleic acid encoding a polypeptide of interest into a target genomic locus in a cell or a population of cells in a subject, methods of expressing a polypeptide of interest from a target genomic locus in a cell or a population of cells in a subject, methods of treating an enzyme deficiency in a subject in need thereof, and methods of preventing or reducing the onset of a sign or symptom of an enzyme deficiency in a subject in need thereof. Some methods, such as when a subject has preexisting against an immunogen to be administered, use plasma cell depleting agents or combinations comprising plasma cell depleting agents to mitigate immune response and facilitate redosing of nucleic acid constructs encoding a polypeptide of interest and nuclease agents targeting a target genomic locus to achieve, for example, a step-wise increase in expression of a polypeptide of interest in a subject following insertion of the nucleic acid construct without overshooting. Other methods, such as when a subject has no preexisting immunity against an immunogen to be administered, use B cell depleting agents (e.g., anti-CD20xCD3 antibody or functional fragment thereof) to mitigate immune response and facilitate redosing of nucleic acid constructs encoding a polypeptide of interest and nuclease agents targeting a target genomic locus to achieve, for example, a step-wise increase in expression of a polypeptide of interest in a subject following insertion of the nucleic acid construct without overshooting.

The invention belongs to the technical field of biochemical engineering, and particularly relates to a Pickering emulsion microcapsule of interface immobilized lipase as well as a preparation method and application of the Pickering emulsion microcapsule. The preparation method of the Pickering emulsion microcapsule comprises the following steps: ultrasonically dispersing silicon oxide nanoparticles which have an emulsifying function and can adsorb enzyme into an organic solvent to serve as an oil phase, dissolving lipase into a buffer solution to serve as a water phase, mixing the water phase and the oil phase, and carrying out ultrasonic dispersion to obtain the Pickering emulsion microcapsule. And then adding a silane coupling agent, organic amine and an organic solvent, and carrying out interface gel-sol treatment to obtain the Pickering emulsion microcapsule. The Pickering emulsion microcapsule disclosed by the invention has better high temperature resistance and pressure resistance, and can realize efficient preparation of epoxide in a fixed bed continuous enzyme catalysis olefin epoxidation reaction.

A method of reducing the toxicity of a trichothecene mycotoxin such as deoxynivalenol (DON) comprises exposing the trichothecene mycotoxin to a UDP- glycosyltransferase enzyme originating from a strain of Bacillus subtilis. The enzyme catalyzes the conversion of the trichothecene mycotoxin to a non-toxic monoglycosylated metabolite. The enzyme is useful in reducing the toxicity of animal feed or food products which would otherwise be contaminated with the trichothecene mycotoxin.

The present invention relates to: a stabilizing complex composition for maintaining the activity of a protein, which can stably maintain an extracellular superoxide dismutase (EC-SOD) protein even at high temperatures for a long period of time; and a preparation method therefor. The present invention is characterized by mixing the EC-SOD protein with ectoine, human recombinant collagen (collagen-1), hyaluronic acid, or sorbitol alone or a mixture thereof, as an active ingredient.

The invention discloses a polypeptide ligand for improving the thermal stability of MMLV reverse transcriptase and application of the polypeptide ligand, and relates to the technical field of biology. The invention specifically discloses a polypeptide ligand with an amino acid sequence as shown in SEQ ID NO: 1. The polypeptide ligand can obviously improve the thermal stability of MMLV RT. After being combined with the polypeptide ligand, the MMLV RT can effectively maintain the activity of the MMLV RT at 45-60 DEG C and maintain good activity in a freeze-thaw cycle, and the Ct value change is small after the MMLV RT is placed at 37 DEG C for more than 15 days, so that the polypeptide has a very good application prospect in industrial production and commercial application.

Disclosed herein are system-related compositions based on clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated (Cas) 9 and methods of using the system-related compositions based on CRISPR / Cas9 for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using the compositions for altering gene expression and genome engineering in muscles such as skeletal muscle and cardiac muscle.

The invention relates to the technical field of preparation of small molecule peptides, and particularly discloses a preparation process of selenium-rich antioxidant pig lung polypeptide. The preparation process of the selenium-rich antioxidant pig lung polypeptide comprises the following steps: cleaning and decontaminating fresh pig lung, stripping trachea, removing grease, and mincing into meat paste to obtain a pig lung prefabricated material; mixing the pig lung prefabricated material with a vitamin C aqueous solution, adding nano silicon dioxide powder and eugenol into the mixture, performing ultrasonic treatment, cooling to-10 to-30 DEG C, preserving heat for 1-2 hours, heating to room temperature, performing homogenizing treatment, filtering, and collecting precipitates to obtain pretreated pig lungs; adding water into the pretreated pig lung, uniformly stirring, adjusting the pH value of the system, adding stability-enhancing alkaline protease for treatment, adjusting the system to be neutral, adding neutral protease for enzymolysis, and cooling to room temperature to obtain pig lung enzymatic hydrolysate; and carrying out centrifugation and ultrafiltration on the pig lung enzymatic hydrolysate, collecting permeate, carrying out nanofiltration, collecting concentrated solution, and drying.

The present invention provides an enzyme preparation for laundry detergent that can effectively improve the washing effect. The enzyme preparation for laundry detergent includes a protease component, a lipase component, and polyethylene glycol. The protease component includes a protease and a protease carrier for immobilizing the protease. The protease carrier includes a porous nanomaterial and polydopamine formed on the surface of the porous nanomaterial. The lipase component includes a lipase and sodium polyoxyalkylene sulfosuccinate. In the enzyme preparation for laundry detergent provided in this solution, the protease and the lipase can maintain good activity for a long time. Therefore, when the enzyme preparation for laundry detergent provided in this solution is applied to the preparation of laundry detergent, the washing effect of the laundry detergent can be effectively improved.

An object of the present invention is to provide a lactase solution showing good permeation through a filtration filter, a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity, and a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity even when a lactase solution having a low protease activity and a low arylsulfatase activity as contaminant enzymes is used.A lactase solution containing any of (i) to (iii) below.(i) 0.0001 to 0.1 mass % of an unsaturated fatty acid or a salt thereof(ii) 0.0001 to 0.1 mass % of a saturated fatty acid salt(iii) 0.01 to 10 mass % of at least one selected from yeast extract, soybean peptone, pea protein, a casein degradation product, and corn steep liquor

The invention provides a method of preparing a biocompatible hydrogel, a hydrogel obtainable by that method, a biocompatible hydrogel for non-covalent immobilization of one or more enzyme(s), and a composition including any of those hydrogels. The invention further provides a method for encapsulating one or more enzyme(s) in a hydrogel as described herein and the use of any of said hydrogels for non-covalent immobilization of one or more enzyme(s) in the hydrogel or the use of any of said hydrogels in a biosensor. Additionally, the present invention provides a kit containing the composition or the hydrogel according to the present invention.

The invention provides liquid deamidase compositions comprising mono- or disaccharide(s), which exhibit high enzymatic stability after storage.

The invention discloses a bacterium-enzyme mixed preparation for PET plastic degradation and a preparation method thereof, and belongs to the technical field of enzyme preparations, a lanthanum metal framework is formed, loaded on the surface of titanium dioxide and carbonized to obtain a lanthanum-doped porous carbon layer, and then nitric acid treatment and silane coupling agent treatment are performed to significantly increase the specific surface area and roughness, so that the specific surface area is increased; the calcium-based diatomite is taken as a carrier to form a sodium alginate microcapsule taking an enzyme preparation as an inclusion, and the sodium alginate microcapsule is loaded on the surface of the calcium-based diatomite, so that the situation that titanium dioxide generates hydroxyl free radicals in photocatalysis to directly attack the enzyme preparation is avoided, and the degradation effect on the PET plastic is prolonged; calcium ions in the calcium-based diatomite can increase the crosslinking degree with the sodium alginate, the load rate of the sodium alginate microcapsule is increased, the strength of the microcapsule is improved, and the slow release effect of an enzyme preparation in the microcapsule is further improved.

The invention belongs to the technical field of bioengineering and food safety, and particularly relates to a fusion enzyme for degrading aflatoxin B1 (AFB1) and / or zearalenone (ZEN) and application of the fusion enzyme. The fusion enzyme S1-AsDPP III disclosed by the invention can effectively degrade AFB1 and ZEN, the fusion enzyme is obtained by fusing a section of self-assembled amphiphilic oligopeptide S1 sequence at the N end of wild type AsDPP III, and the thermal stability of the fusion enzyme and the amphipathy of the fusion enzyme in an oil-water coexistence system can be remarkably improved. Under mild reaction conditions, the fusion enzyme can efficiently degrade AFB1 and ZEN in vegetable oil, and is especially suitable for detoxification treatment of common edible oil such as peanut oil and corn oil. The invention provides a green, safe and efficient scheme for removing fungaltoxin from grease food, and the method has a good industrial application prospect.

The invention relates to the technical field of molecular biology, and provides a DNA origami structure with enhanced stability in a DNA polymerase environment as well as preparation and application of the DNA origami structure. An optional number of conventional staple chains; one or more anti-enzymolysis staple chains, wherein the anti-enzymolysis staple chain comprises a staple section and a suspension section for preventing DNA polymerase degradation; wherein staple sections of the skeleton chain, the conventional staple chain and the anti-enzymolysis staple chain form a DNA origami body, and the DNA origami body comprises a plane or curved surface structure; in addition, the hanging section of the anti-enzymolysis staple chain is exposed out of the DNA origami body, and the hanging section is provided with a polyT block. The stability of the DNA origami structure in a DNA polymerase environment is effectively improved, characterization and analysis are facilitated, and the application range of the DNA origami structure in different enzyme systems is widened.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present invention relates to a semi-fermented zero-sucrose monk fruit concentrated juice, comprising the following ingredients: glucose, fructose, erythritol, mogroside V, water, and the mass ratio of glucose, fructose, and erythritol is 1-10:2-5:1, and the sugar content of the semi-fermented zero-sucrose monk fruit concentrated juice is 50-80 brix; the preparation process of the semi-fermented zero-sucrose monk fruit concentrated juice includes enzymolysis and fermentation, and the fermentation end point is to control the mass ratio of glucose and erythritol to be 1-10:1. After the invertase is modified by trehalose, the present invention allows the enzymolysis to be fully carried out under the synergistic action of the auxiliary reagent citric acid, thereby finally obtaining the zero-sucrose monk fruit concentrated juice. In addition, the present invention controls the degree of fermentation, and when the glucose and erythritol in the system reach a certain ratio, the fermentation is stopped by inactivation, and the GI value of the concentrated juice is further reduced, so as to obtain the semi-fermented zero-sucrose monk fruit concentrated juice containing erythritol.

The invention discloses a laccase mutant as well as preparation and application thereof. The technical problem to be solved is to provide an enzyme mutant with higher enzyme activity and a complex formed by an enzyme with more stable enzyme activity and bacillus subtilis. The protein is any one of the following proteins: B1) a protein with an amino acid sequence shown as a sequence 3; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of the protein B1) or B2) with a protein tag. When the protein is independently used, zearalenone and aflatoxin B1 can be degraded, or when the protein is mixed with bacillus subtilis to prepare a complex, the stability of enzyme can be improved, and the protein can be used for industrial production.

The invention discloses a polypeptide ligand of MMLV reverse transcriptase and application of the polypeptide ligand, and relates to the technical field of biology. The invention specifically discloses a polypeptide ligand with an amino acid sequence as shown in SEQ ID NO: 1, the stability of MMLV reverse transcriptase can be remarkably improved, the MMLV reverse transcriptase can be placed at 37 DEG C for more than 15 days, and the Ct value difference is lower than 2; in addition, the MMLV reverse transcriptase activity can be maintained under a high-temperature condition (45-60 DEG C); the activity loss of the MMLV reverse transcriptase in a freeze-thaw cycle is reduced, and the industrial production and commercial application of the MMLV reverse transcriptase are effectively promoted.

The invention relates to the technical field of pepsin preparation, in particular to a chemical modifier and a method for preparing pepsin through the chemical modifier, a novel composite modifier Mal-PEG-CMC is synthesized, and the modifier with good water solubility and high coupling rate is obtained by optimizing the molecular weight of PEG, the molar ratio of PEG to maleic anhydride, the substitution degree of CMC and the coupling ratio; the chemically modified pepsin is prepared based on the modifier, and the modified pepsin is obtained through the steps of pepsin purification, sulfydryl exposure through DTT pretreatment, targeted modification reaction and purification. The performance of the modified pepsase is remarkably improved, the pepsase is suitable for animal source and recombinant expression pepsase, the application bottleneck of natural enzyme is effectively broken through, and the pepsase has important value in the fields of food processing, medicine and the like.

Provided herein are compositions of conjugates of a guide RNA(s)-CRISPR Cas protein (RNP) complex. The conjugate comprises a guide RNA(s)-CRISPR Cas protein (RNP) complex and one or more molecules selected from PEG, non-PEG polymers, ligands for cellular receptors, lipids, oligonucleotides, polysaccharides and peptides and chemically linked to the Cas protein and / or guide RNA(s). Also provided a guide RNA-DNA template conjugate comprises a DNA template with 5-methylcytosines at specific positions for epigenome editing by DNA methylation at a single nucleotide resolution level. The conjugates are delivered to targeted cells as RNP complexes, or formed in targeted cells from guide RNA conjugates and an mRNA or a viral vector encoding a Cas protein, or formed in targeted cells from a crRNA conjugate(s) and a viral vector encoding both a Cas protein and a tracrRNA. Also provided are preparation methods and uses of these conjugates.

The invention discloses an anti-Taq DNA polymerase antibody or an antigen binding fragment thereof and application thereof, and relates to the technical field of biology. The antibody or the antigen binding fragment of the antibody can be specifically bound with Taq DNA polymerase, the polymerization activity and / or excision activity of the Taq DNA polymerase are / is blocked, and the stability of the Taq DNA polymerase is improved.

The present invention relates to aqueous compositions, and more specifically aqueous compositions for replicating a nucleic acid template, wherein the composition comprises D2O, as well as to methods and uses relating to such compositions. The composition can be used, for example, in a method of storing the composition, or a method for replicating a nucleic acid template, for instance for the synthesis of Xpandomers for sequencing by expansion.