Antibody conjugates

a technology of conjugates and antibodies, applied in the field of antibodies conjugates, can solve the problems of inactivation of either or both antibodies and enzymes, methods that do not address loss of adverse steric effects, etc., and achieves the effects of high antibody specificity and enzyme activity, enhanced detection performance, and intense staining

a technology of conjugates and antibodies, applied in the field of antibodies conjugates, can solve the problems of inactivation of either or both antibodies and enzymes, methods that do not address loss of adverse steric effects, etc., and achieves the effects of high antibody specificity and enzyme activity, enhanced detection performance, and intense staining

US20060246523A1Inactive Publication Date: 2006-11-02VENTANA MEDICAL SYST INC
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  • Antibody conjugates
  • Antibody conjugates
  • Antibody conjugates

Examples

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Embodiment Construction

[0043] Further aspects of the invention are illustrated by the following non-limiting examples, which proceed with respect to the abbreviations and terms defined below.

[0044] I. Abbreviations [0045] 2-ME—2-mercaptoethanol [0046] 2-MEA—2-mercaptoethylamine [0047] Ab—antibody [0048] ALP—alkaline phosphatase [0049] BSA—bovine serum albumin [0050] DTE—dithioerythritol(cis-2,3-dihydroxy-1,4-dithiolbutane) [0051] DTT—dithiothreitol(trans-2,3-dihydroxy-1,4-dithiolbutane) [0052] EGFR—epidermal growth factor receptor [0053] ER—estrogen receptor [0054] HRP—horseradish peroxidase [0055] IHC—immunohistochemistry [0056] ISH—in situ hybridization [0057] MAL—maleimide [0058] NHS—N-hydroxy-succinimide [0059] PEG—polyethylene glycol [0060] PR—progesterone receptor [0061] SAMSA—S-Acetylmercaptosuccinic acid [0062] SATA—N-succinimidyl S-acetylthioacetate [0063] SATP—Succinimidyl acetyl-thiopropionate [0064] SM—signal-generating moiety [0065] SMPT—Succinimidyloxycarbonyl-α-methyl-α-(2-pyridyldithio)to...

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Abstract

Antibody / signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.

Description

RELATED APPLICATION DATA [0001] This application claims the benefit of U.S. Provisional Patent Application No. 60 / 675,759, filed Apr. 28, 2005, which application is incorporated by reference herein.BACKGROUND OF THE INVENTION [0002] 1. Field [0003] The present invention relates to reagents and methods for detecting a molecule of interest in a biological sample. More particularly, the present invention relates to antibody conjugates and methods for using such conjugates to detect a molecule of interest in a biological sample such as a tissue section. [0004] 2. Background [0005] Covalent conjugates of antibodies and signal-generating moieties can be used in immunoassays for detecting specific target molecules in biological samples. The antibody portion of such conjugates specifically binds to a target in the sample and the signal-generating moiety is utilized to provide a detectable signal that indicates the presence / and or location of the target. One type of conjugate that has become...

Claims

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Application Information

Patent Timeline
02 Nov 2006
Publication
US20060246523A1
IPC
G01N33/53; C12N9/00; C07K16/18; C07K16/46
CPC
A61K47/48338; A61K47/4843; G01N33/535; C12N9/16; C07K16/44; C12N9/0065; C07K16/42; A61K47/65
Inventors
BIENIARZ, CHRISTOPHER; WONG, JENNIFER