186 results about "Horseradish peroxidase" patented technology

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention relates to a kit for detecting pepsinogen I and a preparation method of the kit, and relates to the technical field of biology. The kit comprises an enzyme marker, a luminous marker, a luminous auxiliary agent and a triggering agent, the enzyme marker comprises an anti-PG I monoclonal antibody marked by horse radish peroxidase; the luminous marker comprises an anti-PG I monoclonal antibody marked by 9, 10-dihydroacridine; the light-emitting auxiliary agent comprises H2O2. According to the present invention, the spatial proximity chemiluminescence analysis method is adopted, and the carrier and the washing process are not required, such that the kit cost is reduced, and the detection time is shortened; the method adopts homogeneous reaction, so that the detection result is more real and credible.

The invention belongs to the technical field of biomedical materials and tissue regeneration, and particularly discloses hydrogel with a self-enhanced piezoelectric effect as well as a preparation method and application of the hydrogel. The hydrogel is prepared by taking microphone (MXene), cellulose nanofiber (CNF), tyramine grafted carboxymethyl cellulose (T-CMC) and regenerated silk fibroin (RSF) as main materials through an enzyme crosslinking reaction of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The hydrogel has excellent mechanical properties, biocompatibility and safety, and can realize self-enhancement in vivo and generate stable piezoelectric signals. The piezoelectric stimulation can promote proliferation, migration and differentiation of osteogenesis-related cells and regulate and control a bone tissue microenvironment, so that formation and repair of new bones in a bone defect area are remarkably accelerated. The hydrogel can be used as an injectable or moldable bone tissue repair material, and is suitable for bone fracture, bone defect and other bone tissue regeneration scenes.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention discloses a novel method for rapidly detecting ochratoxin A based on an electrochemical deposition polydopamine coating modified gold electrode, and belongs to the technical field of analytical chemistry and biology. The method is based on a polydopamine coating, is combined with an ochratoxin A aptamer with high specificity and high affinity, introduces a DNA-modified nanogold compound as a signal carrier and a signal amplification element, and realizes high-sensitivity detection of ochratoxin A through current change generated by catalyzing H2O2 / TMB by horse radish peroxidase captured on the surface of an electrode.

The invention provides a drug-loaded sophorolipid microsphere, a preparation method thereof and application of the drug-loaded sophorolipid microsphere in preparation of a bionic cartilage scaffold. The preparation method of the drug-loaded sophorolipid microspheres comprises the following steps: (1) preparing a drug-containing sophorolipid solution; (2) preparing multiple emulsion; and (3) adding a horse radish peroxidase solution into the multiple emulsion for reaction to obtain the drug-loaded sophorolipid microspheres. The bionic cartilage scaffold sequentially comprises a bone layer, a transition layer and a cartilage layer from bottom to top, the bone layer is a cross-linked compound of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the transition layer is a physical mixture of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the cartilage layer is drug-loaded sophorolipid microspheres. The drug-loaded sophorolipid microspheres disclosed by the invention have good biocompatibility, excellent biocompatibility and good mechanical properties. The bionic cartilage scaffold utilizes the characteristics of the nHA and the drug-loaded sophorolipid microspheres to form a unique network structure, so that material failure caused by mechanical mismatching is avoided.

Nucleotide compositions, recombinant vectors, and recombinant viral genomes are disclosed that include a modified horseradish peroxidase (HRP) gene sequence, as well as recombinant viruses that have the modified HRP gene sequence incorporated into the viral genome thereof. The recombinant viruses are utilized in an HRP-based assay to analyze the neutralization potential of candidate antiviral agents, such as antiviral antibodies.

The present invention relates to the field of biomedical analysis, and discloses a CHO-S cell residual protein detection kit and a preparation method thereof, including an enzyme-labeled plate coated with an anti-HCP protein antibody, biotin-labeled IgG, streptavidin labeled with horseradish peroxidase, and an auxiliary preparation. The usage method of the kit includes the following steps S1: coating the enzyme-labeled plate with a CHO HCP antibody; S2: adding a sample to be detected and a standard solution to the coated enzyme-labeled plate, incubating and washing; S3: adding biotin-labeled IgG to the enzyme-labeled plate washed in S2, incubating and washing; S4: adding streptavidin labeled with horseradish peroxidase to the enzyme-labeled plate washed in S3, incubating and washing; S5: adding a chromogenic solution to the enzyme-labeled plate washed in S4 for color development, incubating, adding a termination solution to terminate the reaction, and measuring the absorbance value at a wavelength of 450 nm. The kit makes up for the blank of a special HCP detection kit, and has high specificity and high detection precision for HCP detection.

The present invention discloses an injectable antibacterial hemostatic hydrogel dressing and its preparation method and application. The raw materials for its preparation have excellent biocompatibility, and the hydrogen peroxide / horseradish peroxidase catalytic oxidation system adopted can provide a mild, efficient and highly biocompatible cross-linking process for the hydrogel dressing. The hydrogel dressing prepared by this method has excellent antibacterial and antioxidant properties, high strength and high tissue adhesion strength, and has an excellent hemostatic effect, and can be well applied to the field of skin trauma treatment.

The application discloses a fucose glycoprotein detection kit, a preparation method thereof and a fucose glycoprotein color developing method. First, a liquid sample is dropped on the surface of the kit, and the sample and test paper are infiltrated through a sample inlet. Under the action of transverse flow force, the sample flows to an observation window along a hydrophilic test paper. The glycoprotein containing alpha 1,2-fucose in the sample is combined with agglutinin 1, and the rest of the sample continues to flow to the next layer of test paper. Then, the glycoprotein containing alpha 1,3-fucose is combined with agglutinin 2, and the rest of the sample flows to the third layer and is combined with agglutinin 3 containing alpha 1,6-fucose glycoprotein. All the combined fucose glycoproteins are combined with agglutinin biotin through their antibodies, and horseradish peroxidase is used for labeling color development, so that quantitative detection of the fucose glycoprotein in the sample is realized. The method has important significance for studying the changes of different connection fucose glycoproteins in cells and body fluids in diseases.

The invention relates to a touch fluid neuron specific tracing imaging agent, a preparation method and application, and belongs to the technical field of nuclear medicine imaging, the tracing imaging agent is composed of a nerve tracer agent horseradish peroxidase (HRP), zirconium-89 isotope chelated by deferoxamine and a pharmaceutically acceptable carrier, the effective tracking window of the tracer agent is prolonged to 120 hours, and the effective tracking window of the tracer agent is prolonged to 120 hours. The multi-stage synaptic transfer period of the fluid touching neuron can be completely covered; the spatial resolution is improved by 5-8 times compared with that of traditional FDG-PET, and a fluid touching neuron cluster can be accurately presented; the in-vitro serum stability is excellent, the radiochemical purity is still larger than or equal to 80.5% within 120 hours, PET / MRI bimodal imaging can be adapted, drug administration is conducted through lateral ventricle minimally invasive injection, the probe is suitable for various animal models such as mice, rats and non-human primates, in-vivo dynamic tracing of sympathetic fluid neuron trans-synapses can be achieved on the premise that no tissue damage exists, and the probe has a good application prospect. And a key technical tool is provided for neural circuit analysis, neuroscience basic research and preclinical research of neurodegenerative diseases.

The application provides a glucose detection test strip and a preparation method and application thereof. The preparation method of the glucose detection test strip comprises the following steps: taking copper sulfate pentahydrate, a phosphate buffer solution, glucose oxidase and horseradish peroxidase, mixing uniformly to obtain an enzyme-containing mixed solution; immersing a test strip in the enzyme-containing mixed solution, and obtaining an organic-inorganic hybrid nanoflower loaded test strip after reaction; immersing the organic-inorganic hybrid nanoflower loaded test strip in a mixed solution containing a chromogenic agent, a gel, bovine serum albumin and polyethylene glycol to obtain a glucose detection test strip. The preparation method of the glucose detection test strip has higher sensitivity and shorter response time.

The invention provides a rapid detection kit and a rapid detection method for phytosterol, and belongs to the field of food detection.The rapid detection kit for phytosterol comprises a phytosterol standard substance, a standard substance diluent, a substrate solution, an enzyme mixed solution, a stop solution and an elisa plate; the standard substance diluent comprises isopropanol or dimethyl sulfoxide; the substrate solution is prepared from TMB (Tetramethylbenzidine) and a substrate buffer solution; the enzyme mixed solution is a mixed solution of a cholesterol oxidase solution and a horse radish peroxidase solution. By optimizing the composition of each working reagent in the kit, the sensitivity and accuracy of phytosterol detection are remarkably improved. Meanwhile, the rapid detection method provided by the invention is simple and convenient to operate, short in detection time and suitable for on-site rapid detection.

The application discloses a high-stability horseradish peroxidase, a recombinant expression vector, a preparation method and application. The amino acid sequence of the horseradish peroxidase is shown as SEQ ID NO. 1. Compared with wild-type horseradish peroxidase, the enzyme activity of the horseradish peroxidase is increased by 3.2 times, and compared with commercially available horseradish peroxidase, the thermal stability is better. The method can produce the horseradish peroxidase by using a tobacco transient expression system. Compared with an Escherichia coli expression system and a yeast expression system, the tobacco transient expression system can correctly fold and glycosylate, and has higher enzyme activity and stability. The purity of the horseradish peroxidase obtained according to the method can reach RZ 3.2, the enzyme activity can reach 275 U / mg, and the horseradish peroxidase can be applied to immunological detection.

The present application relates to the technical field of in vitro diagnosis, in particular to a kit and a preparation process thereof, the present application is a recombinant virus antigen of coxsackie virus A16 and EV71, and is used for specific detection of coxsackie virus A16 IgM, a magnetic micro-particle chemiluminescence kit, which comprises magnetic particles coated with mouse anti-human IgM monoclonal antibody, coxsackie virus A16 antigen labeled with horseradish peroxidase and coxsackie virus EV71 recombinant virus antigen. The reagent has excellent sensitivity, specificity and precision, and can distinguish the two pathogens of EV71 and coxsackie A16 in hand-foot-mouth disease, has high repeatability between tests, and is easy to control the standard.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The invention provides photo-thermal response enhanced hydrogel loaded with black rice extract as well as a preparation method and application of the photo-thermal response enhanced hydrogel loaded with the black rice extract, and the photo-thermal response enhanced hydrogel is formed by taking a mixture of hyaluronic acid / tyrosine conjugate and the black rice extract as a base material under the cross-linking action of horse radish peroxidase / catalase, horseradish peroxidase acts on the black rice extract while serving as a cross-linking agent, the near-infrared photo-thermal responsiveness of the black rice extract after reaction of the horseradish peroxidase is greatly enhanced, and good oxidation resistance, inflammation resistance and photo-thermal responsiveness can be achieved only through a small amount of black rice extract in a hydrogel system.

The application discloses a urine tyrosine detection system, a detection method and a detection kit. The detection system comprises a buffer solution, 4-aminoantipyrine, horseradish peroxidase and hydrogen peroxide, and the pH of the detection system is 6.5-7.8. After the sample to be detected is placed in the detection system and reacts at room temperature, the quantitative analysis of tyrosine can be realized by observing the color change of the reaction solution or testing the absorbance value of the reaction solution at 485 nm. Compared with the traditional Millon reaction and the multi-enzyme reaction system, the reagent used in the application has no toxicity and corrosion, does not cause hidden troubles to human health and environmental safety, is simple to operate, does not need special instruments and has low cost, and is conducive to realizing 'home health detection'.

The invention discloses a fibroin-based hydrogel optical fiber as well as a preparation method and application thereof, and belongs to the technical field of hydrogel optical fibers. According to the preparation method, the fibroin-based hydrogel optical fiber with a chemical-physical double-crosslinking structure is constructed through a synergistic strategy of enzymatic crosslinking and ethanol solution post-treatment; the preparation method comprises the following steps: performing degumming treatment by using a sodium carbonate solution to prepare a silk protein solution, respectively preparing a core solution and a coating solution by using the silk protein solution, a horseradish peroxidase solution and an H2O2 solution, injecting the core solution into gel, then using an ethanol solution to reinforce the mechanical properties of the core solution, and then coating the coating solution, thereby obtaining the high-strength and high-toughness fiber. Finally, the fibroin-based hydrogel optical fiber with excellent mechanical properties, low optical loss and good biocompatibility is obtained.

The invention belongs to the technical field of biological medicine, and particularly relates to a detection kit for detecting new coronavirus. According to the invention, a new coronavirus NP protein is taken as an immunogen, monoclonal antibodies aiming at specific epitopes of the new coronavirus NP protein are screened, a specific strain is taken as a capture antibody, the other strain is labeled by horse radish peroxidase and taken as a detection antibody, and a double-antibody sandwich ELISA method for detecting the SARS-CoV-2 NP protein is established. Compared with the prior art, the method has the advantages that the detection time is greatly shortened, the detection can be completed in only 6 hours, and a foundation is laid for rapid detection of an SARS-CoV-2 clinical sample.

The invention discloses a system for detecting ArsM in a sample, a detection method, a detection device and application thereof. The detection system of the present invention comprises a clustered regularly spaced short palindromic repeat (CRISPR)-Cas complex comprising a guide RNA targeting ArsM and a CRISPR-related (Cas) protein or a functional fragment thereof; and a horse radish peroxidase (HRP) complex to which a single-stranded DNA (ssDNA) is linked. According to the invention, a cascade amplification detection system based on CRISPR / Cas12a is constructed, and the cascade amplification detection system is further integrated on a multi-channel paper-based microfluidic device to realize on-site high-throughput analysis of nucleic acid molecules.

The invention discloses bone repair magnetic silk fibroin hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing an injectable hydrogel matrix, superparamagnetic iron oxide nanoparticles, horse radish peroxidase and a hydrogen peroxide solution to obtain a component A; mixing alendronate sodium, calcium chloride, zinc chloride and sodium hydroxide to obtain a component B; and mixing the component A and the component B to obtain the bone repair magnetic silk fibroin hydrogel. The innovation of the invention lies in that the magnetic field oriented assembly and the chemical mineralization process are organically fused for the first time, and the double breakthrough of the mechanical strength and the biological activity of the material is realized through the multi-physical field coupling effect. The magnetic enhanced mineralization strategy not only overcomes the technical bottlenecks of insufficient viscosity and low mineralization efficiency of traditional hydrogel, but also provides a solution for accurate repair of complex bone defects by constructing a magnetic responsive bone regeneration microenvironment, and shows great clinical transformation potential.

The application belongs to the field of material preparation, and particularly relates to a method for in-situ synthesis of microcapsules by enzyme catalysis and application thereof in anticorrosive coatings. The method specifically comprises the following steps: firstly, preparing a cellulose nanocrystal dispersion liquid and an emulsion of flaxseed oil containing cembrene; ultrasonic dispersing the emulsion, and then performing magnetic stirring to obtain a Pickering emulsion; adding hydrogen peroxide and horseradish peroxidase solution into the Pickering emulsion, and stirring and reacting; and performing freeze-drying on the emulsion after the reaction is completed to obtain cellulose nanocrystal microcapsules. The application uses an enzyme catalysis method to prepare microcapsules with an anticorrosive function, and the synthesized microcapsules do not contain harmful substances such as formaldehyde and are environmentally friendly. The synthesized microcapsules have good stability, can be easily dispersed in an aqueous solution, can be used as a carrier of healing reagents for water-based anticorrosive coatings, and have a simple preparation method, controllable size and suitability for large-scale production.

The present invention belongs to the technical field of mycotoxin detection, and particularly relates to a kit and a detection method for detecting vomitoxin. The kit includes an antigen, a primary antibody, an enzyme-labeled secondary antibody, a chromogenic regulator, a phosphate buffer solution, and a detection reagent; the antigen is bovine serum albumin conjugated with vomitoxin; the primary antibody is a monoclonal antibody against vomitoxin; the enzyme-labeled secondary antibody is a goat anti-mouse IgG secondary antibody labeled with horseradish peroxidase; the chromogenic regulator is ethylenediaminetetraacetic acid or its sodium salt, or vitamin C; the detection reagent includes a TMB chromogenic solution. The present invention uses ethylenediaminetetraacetic acid or its sodium salt as a chromogenic regulator to change the chemical microenvironment around the chromogenic substance, enhance the optical properties of the chromogenic substance, and thereby reduce IC 50 , and improve the detection sensitivity.

The present invention discloses a lactic acid detection kit and a preparation method thereof, which belongs to the technical field of detection reagents. The kit comprises 3 mL of R1 reagent, 1 mL of R2 reagent and 1 mL of standard product. The R1 reagent contains bovine serum albumin, nanosilver-trehalose complex and PEG4000. The bovine serum albumin can prevent enzyme decomposition and nonspecific adsorption, chelate a small amount of dissociated Ag, and + , preventing competition with H2O2 for the binding sites of horseradish peroxidase and ensuring the stable generation of quinoneimine; PEG4000 plays a dispersing role, assisting the nanosilver-trehalose complex to form a protective film on the surface of lactate oxidase and horseradish peroxidase, protecting the protein structure of the two enzymes, and can capture electrons from the surrounding environment, increasing the electron transfer efficiency of the active centers of the enzymes during the catalytic process of the two enzymes, thereby further increasing the activity of the two enzymes and improving the sensitivity and accuracy of the kit.

The invention discloses a DNA framework nucleic acid cascade enzyme, a paper-based colorimetric sensor and a preparation method and application of the paper-based colorimetric sensor, and belongs to the technical field of biological detection.The DNA framework nucleic acid cascade enzyme comprises a first enzyme, a second enzyme, a first DNA tetrahedron and a second DNA tetrahedron; the first enzyme is glucose oxidase with the surface modified by DBCO-pri, and the second enzyme is horse radish peroxidase with the surface modified by DBCO-pri; the first enzyme and the first DNA tetrahedron as well as the second enzyme and the second DNA tetrahedron are respectively connected through base complementary pairing; the first DNA tetrahedron and the second DNA tetrahedron are hybridized through a nucleic acid connecting chain to form a dimer structure. According to the invention, the DNA nanostructure is used for accurately fixing enzyme molecules and controlling the spatial arrangement of the enzyme molecules, the cascade catalytic efficiency is improved, and the advantages of low cost and portability of a paper-based platform are combined, so that high-sensitivity, rapid and visual detection of sweat glucose is realized.

The application relates to the technical field of materials, in particular to a monodisperse core-shell microsphere and a preparation method thereof. The method comprises the following steps: S1, using a dispersion phase liquid containing a degradable polymer, forming monodisperse degradable polymer microspheres through a microfluidic process or an emulsification method; S2, combining horseradish peroxidase through one or more of the following methods: dopamine self-polymerization, electrostatic adsorption and chemical grafting on the surface of the degradable polymer microspheres, and under the catalysis of the horseradish peroxidase, crosslinking and coating the degradable polymer microspheres with a hydrogel material to obtain monodisperse core-shell microspheres with a hydrogel layer; the hydrogel material is a hydrogel system that can be crosslinked by enzyme initiation, such as hydroxyphenylpropionic acid hydrogel, silk fibroin or hyaluronic acid hydrogel. Through the method, highly monodisperse degradable core-shell microsphere materials can be obtained, the process operation is simple, and the method is beneficial to wide popularization and application.

The invention provides a nano-enzyme system based on an Ag-polydopamine nano-microsphere (at) MOF-74 structure as well as a preparation method and application of the nano-enzyme system, and belongs to the technical field of biological medicines. The nano-enzyme system takes a polydopamine microsphere as a core, and an MOF-74 shell layer grows on the surface of the polydopamine microsphere in situ to form a composite microsphere with a core-shell structure; and by utilizing the reduction property of polydopamine, Ag nanoparticles are reduced in situ and uniformly dispersed in the MOF-74 framework, so that the composite nano material with the enzyme-like activity and the photo-thermal function is constructed. The system shows excellent horse radish peroxidase-like activity, and can effectively promote electron transfer between hydrogen peroxide and a substrate and catalyze a chromogenic reaction. Meanwhile, the composite system has good photo-thermal conversion performance, shows a remarkable photo-thermal sterilization effect on gram-negative bacteria and gram-positive bacteria, and can effectively inhibit and destroy the formation of bacterial biofilms. An in-vitro antibacterial experiment result shows that the MOF-74-based nano enzyme system has relatively high antibacterial activity.

The application discloses a silk protein-based hydrogel optical fiber and a preparation method and application thereof, and belongs to the technical field of hydrogel optical fibers.The silk protein-based hydrogel optical fiber with a chemical-physical double crosslinking structure is constructed through a synergistic strategy of enzymatic crosslinking and ethanol solution post-processing; firstly, a degumming treatment is performed by using a sodium carbonate solution to prepare a silk protein solution; then, a core liquid and a coating liquid are respectively prepared by using the silk protein solution, a horseradish peroxidase solution and an H2O2 solution; the core liquid is injected into a gel, and then, an ethanol solution is used to strengthen the mechanical property of the core liquid; finally, the coating liquid is coated, and the silk protein-based hydrogel optical fiber with excellent mechanical property, low optical loss and good biocompatibility is obtained.