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Electrochemcial immunoassay for tumor marker and small size immunoassay chip

A tumor marker, immunoassay technology, applied in biological testing, measuring devices, scientific instruments, etc., can solve the problems of little involvement, difficulty in popularization, and high price of immunoassay, so as to improve the detection speed and efficiency, and reduce the cost of the assay. , the effect of low cost

Inactive Publication Date: 2005-05-11
NANJING UNIV +1
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  • Abstract
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Problems solved by technology

However, this technology is currently mainly used for gene analysis and expression research, such as CN03126932.X involves a biochip to detect tumor genes, and it is rarely involved in immune detection
Due to the needs of clinical diagnosis, protein chips for tumor marker detection have attracted attention, but it has been reported that the method is expensive and difficult to popularize
Miniature portable detection instruments and related detection chips for immunoassay of serum tumor markers for early diagnosis of malignant tumors have not been reported yet

Method used

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  • Electrochemcial immunoassay for tumor marker and small size immunoassay chip
  • Electrochemcial immunoassay for tumor marker and small size immunoassay chip

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Embodiment Construction

[0025] 1.1 Preparation of disposable immune chip

[0026] Disposable separation-free microvolume immunoassay chip consists of figure 1 components shown. The ring-shaped carbon electrode has an inner diameter of 3 mm, an outer diameter of 7 mm, and a thickness of 5 mm. The inner wall of the ring-shaped carbon electrode is modified with an electron mediator 6 as a working electrode. The functionalized membrane 9 immobilized with the antigen molecule to be tested is prepared on a PVC membrane, and the membrane is cut into a circle with an outer diameter of 2.5 mm and assembled on the substrate 8 . After the substrate 8 is overlapped with the substrate 7, the annular carbon electrode is assembled on the substrate 7 to form a micro-reaction chamber with a volume of about 40 μL. Finally, the upper cover 2 integrating the Ag / AgCl reference electrode and the carbon counter electrode is fixed on the top of the annular carbon electrode, so that the bottom of the micro-reaction chamber...

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Abstract

An electrochemical method for determining immunity of tumor marker includes forming microreaction cell, placing reference and counter electrode above it and fixing antigen molecular functioning film at pool bottom, connecting enzyme labelled antibody to pool bottom at time of only proper quantity of horseradish peroxidase to label tumor marker antibody, obtaining cataltyic current on working electrode by contacting free immune matter with electronic media and having positive ratio of the current to antigen. The microvolume immune determining chip is also disclosed.

Description

technical field [0001] The invention relates to a tumor marker immunoassay method and a microvolume immunoassay chip thereof, in particular to a tumor marker chip immunoassay method established by electrochemical analysis technology. Background technique [0002] Immunoassay is a highly sensitive and highly selective method for the determination of trace substances using antigen / antibody reactions, and it provides a powerful means for the clinical immunoassay of serum tumor markers. Usually, the clinical immunoassay methods for serum tumor markers mostly use the "double antibody sandwich" method, such as radioimmunoassay, enzyme labeling, time-resolved fluorescence, chemiluminescence and electrochemiluminescence. Because of its convenient labeling, high sensitivity, less interference and wide application range, radioimmunoassay was once the most commonly used method in clinical immunology testing. However, it requires radioactive operation, which ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/403G01N33/53
Inventor 戴宗严枫鞠熀先
Owner NANJING UNIV
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