216results about How to "Low assay cost" patented technology

The disclosed electrochemical screening and early diagnostic device for malignant tumor comprises: an immune detection chip (1) connected to a time-resolution multichannel potentiostat (2) that connects to a data process and display system (3) by an interface. Wherein, the chip (1) comprises eight working electrodes fixed different antigen films and Ag wire (a), Ag/AgCl reference electrode (b), a carbon-pair electrode (c), and an insulation film (d). This invention needs low cost, has intelligence and fit to fast detection.

The method comprises: sampling coal core from underground mining; at underground mining, measuring and calculating the gas loss quantity of coal core; at the ground, measuring and calculating the desorption of the coal mining gas; measuring and calculation the grind gas desorption of partial coal core; calculating the content of gas capable of being desorbed.

The invention relates to establishment of a salvianolic acid extract fingerprint spectrum and a content measurement method of related components. The content measurement method includes the following steps: 1) establishing the salvianolic acid extract digitized quantitative fingerprint spectrum through ultrahigh performance liquid multi-wavelength visible ultraviolet detection method; 2) recognizing peaks in the fingerprint spectrum in the step 1) with mass spectrum to determine common fingerprint peaks of ultraviolet detection; and 3) performing measurement to contents of three indicative components, which are high in content, significant in activity and definite in structure, recognized in the step 2) through high performance liquid chromatography variable-wavelength detection method.

The invention provides a method for determining the sinter mixture granularity, which comprises the following steps of: (1) sample collection: sampling samples and determining the sample quantity; (2) sample preparation: reducing, dividing and freezing the samples; (3) screening: selecting screening equipment, determining the screening procedure, determining the screening time and carrying out screening; (4) weighing: weighing the weight of screened materials in all size grades; and (5) size grade calculation: calculating the percentage corresponding to all size grades according to a formula after the weighing completion of the samples in all size grades, recording the percentages, and calculating the average granularity. The method is characterized in that in the second step, the samples are frozen by a refrigerator after reduction and division. When the method is used, the problems that in the existing granularity determining method, the mixture size grade composition under the actual production condition cannot be objectively reflected, the mixed pelletization effect enhancing measure cannot be reasonably evaluated, and the like can be solved. The method has positive effects of effectively improving the proportioning, stabilizing the sinter process and stabilizing and improving the sinter mineral quality on sinter work procedures.

The present invention relates to a rice amylose content and gelatinization temperature quickly synergistic determination method, belonging to the field of rice quality determination technology. Said method includes the following steps: selecting sample to be determined and defining check sample, using KOH solution to make gelatinization, observing cargo rice endosperm decopmosition, recording and calculating its gelatinization temperature, fully staining the sample to be determined and check sample by using I2-KI solution and making comparison so as to quickly judge the content of rice amylose.

The invention relates to a method for detecting protein content. The method comprises the following steps of: preparing standard protein samples and reagents (1), (2) and (3); preparing the standard protein samples with gradient; adding the reagents (1), (2) and (3) into the gradient samples and samples to be tested by a micropore method or a standard test tube method; measuring absorbance; drawing a standard curve; and calculating the concentration according to the absorbance of the samples to be tested. By the method, the protein content can be detected accurately. The method is applicable to protein solution containing a plurality of interfering substances. By the method, the inclusiveness for the interfering substances such as a surfactant, a reducing agent, a chelating agent, a nitrogen-containing compound and the like in the protein analysis and detection process can be improved. The method is a simple, quick, high-sensitivity and high-stability method for detecting the protein concentration and has a wide application prospect in the field of life science research.

The invention provides a method for judging the source of a colored soluble organic matter based on a fluorescence-spectrum integral specific value. After a three-dimensional fluorescence spectrum of the colored soluble organic matter in a lake is measured, the source of the colored soluble organic matter is distinguished by utilizing the specific value IC to IT between a peak-C spectrum integral value of humic acid and a peak-T spectrum integral value of similar tryptophan; when the IC to IT is less than 5, the source of the colored soluble organic matter is an endogenous dominant; when the IC to IT is not less than 5 but not more than 20, the source of the colored soluble organic matter is an exogenous dominant; when the IC to IT is more than 20, the source of the colored soluble organic matter is an intensive exogenous effect. By using the method provided by the invention, the addition of a chemical reagent is not needed; the carrying out of fussy complicated calculations of the measurement of a conventional chemical index, the parallel factor analysis of a three-dimensional fluorescent component, and the like, is also not needed; the measurement of the fluorescence intensity, in several common waveband ranges, of the colored soluble organic matter is only needed; thus, the quick evaluation and division of the source of the colored soluble organic matter in an inland water body can be realized; the operability is high; the method is easy to popularize and is compared with peer research results at home and abroad; meanwhile, the fluorescence spectrum is easily modified to form an on-line or in-situ monitoring fluorescent probe; therefore, the method has important and high application value.

The invention discloses a rapid and automatic determination method and device for tripolycyanamide content in dairy products, belonging to the field of food analysis. The method comprises the following steps that: in a unit (A) under an operating procedure, a first step, a multifunctional valve is in a state I, a pump A rotates, a pump B stops, a first sample flows into a column 2 for enriching tripolycyanamide, and the eluent flows into a column 1 for regenerating tripolycyanamide; a second step, the pump A stops, the pump B rotates, the cleanout fluid flows into the column 2 for cleaning the impurities therein; a third step, the multifunctional valve is switched to a state II, eluent flows into the column 2 for eluting and regenerating tripolycyanamide; the tripolycyanamide in the column 2 is substituted by the eluent and directly enters into a sampling ring of a unit (B) in the form of a sample plug, so as to perform volume quantification, injection, re-separation and detection, and a response signal is processed by a computer; and meanwhile, a second sample enters into the column 1 for enrichment, and the process of the column 2 is repeated. The method and the device are high in automation degree and excellent in reproducibility, and can analyze about 30 samples per hour; and the unit (A) in the invention can be used with various flow injection analysis systems.

The invention discloses a pre-treatment method for phthalate ester plasticizers in edible oil. The pre-treatment method comprises: carrying out extraction on a certain mass of edible oil by using an organic solvent; carrying out a freezing degreasing treatment on the extraction liquid, and extracting the liquid; carrying out solid phase extraction small column purification on the obtained extraction liquid; and carrying out an elution blow drying volume metering treatment on the purified purification solution. Compare with the pre-treatment method in the national standard, the pre-treatment method of the present invention has the following characteristics that: the inspection process is short, operations are convenient, extraction efficiency is high, repeatability is good, solvent consumption is less, a measurement cost is low, sensitivity is high, contents of five common phthalate esters in edible oil can be concurrently detected, and good application prospects are provided.

The invention discloses a fitting measurement method for coal seam gas content. Firstly, a fitting calculation model is established, and the general expression of the coal sample gas desorption law is deduced according to the calculation. ; Then take samples from the downhole coal core, and measure the atmospheric pressure p of the downhole measurement site sequentially ₁ and temperature T ₁ 1. Downhole natural desorption gas volume; correct the downhole natural desorption gas volume data, and after the temperature and pressure are corrected to the standard state, the gas loss per unit mass of coal core can be calculated. In the present invention, the relative error δ between the gas content obtained by the direct fitting measurement method and the gas content measured by the indirect method is less than 10%, which meets the actual requirements of the site, and the method is feasible; the method can measure a large number of measuring points, and can be better It is widely used in coal seam outburst dangerous working face and regional prediction, pre-extraction gas effect evaluation and mine coal seam gas emission prediction, etc.

The invention provides a method for determining a nickel content in soil by microwave digestion-flame atomic absorption spectrometry, wherein the method is characterized by: pretreating a soil sample by combining long-time immersion and stepedly heated microwave digestion, using polyoxyethylene castor oil glycerin ether as an activator, raising atomization efficiency of nickel atoms in flame, raising determination sensitivity, thereby raising sensitivity; based on linear relation of absorbance and nickel content determined by a flame atomic abserption spectrometer by a standard solution, measuring and calculating to obtain the content of nickel atoms in soil. The method provided by the invention solves problems that a method for determining trace elements by traditional atomic absorption spectrometry has disadvantages long time consumption, low sensitivity, large solvent consumption amount, and severe environment pollution caused by a solvent, and the method provided by the invention has advantages of good precision, high recovery rate, accuracy, reliability, rapidness and convenience.

The invention provides a method for measuring bromide ions and iodide ions simultaneously. According to the method, the bromide ions and the iodide ions in water are simultaneously measured by a dual-wavelength spectrophotometry according to optical adsorption properties of ultraviolet regions of the bromide ions and the iodide ions; an analysis process is simple and quick; the measurement cost is low; the method disclosed by the invention can be used for simultaneous and quick measurement of trace bromide ions and trace iodide ions in systems such as seawater, river water, lake water, underground water, drinking water and bittern.

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.

The invention relates to a method for testing the melting speed of continuous casting powder. The method is characterized by comprising the following steps of: weighing three parts of casting powder samples by using a scale, wherein each part is 1.49-1.51 g; in the event of raising the temperature, placing a crucible in a furnace, raising the temperature of the furnace to 1350 DEG C, wherein the temperature fluctuation error is +/- 20 DEG C, and keeping the constant temperature for 20 min; taking out the crucible from a hearth, rapidly adding a part of casting powder sample to be tested into the crucible, putting the sample together with the crucible in the furnace, simultaneously, stating a stopwatch to time, and recording the time required for completely melting the sample, namely the time for putting the sample in the hearth till the last dark spot is brightened; calculating the melting speed according to the formula MR=W/tau*A; and repetitively testing according to the above steps and calculating other samples, and then solving an average value to obtain the melting speed MR of the casting powder under the 1350 DEG C condition. The invention aims to provide the method, which is capable of simulating the one-way heating and melting condition of the casting powder on a steel level in a crystallizer and true reflecting the melting speed of the casting powder.

The invention discloses a method for determination of calcium sulfite and calcium carbonate content of flue gas desulfurization gypsum and belongs to the method for determination of calcium sulfite and calcium carbonate content. The method comprises respectively dissolving flue gas desulfurization gypsum subjected to full oxidation treatment and unoxidized flue gas desulfurization gypsum by a diluted hydrochloric acid solution, determining mass losses of the flue gas desulfurization gypsum and the unoxidized flue gas desulfurization gypsum by an analytical balance in dissolution, calculating calcium carbonate content of the flue gas desulfurization gypsum according to the mass loss of the flue gas desulfurization gypsum subjected to full oxidation treatment, and calculating total content of calcium sulfite and calcium carbonate of the flue gas desulfurization gypsum according to the mass loss of the unoxidized flue gas desulfurization gypsum, wherein the difference of the calcium carbonate content and the total content of calcium sulfite and calcium carbonate is the total content of calcium sulfite of the flue gas desulfurization gypsum. Calcium sulfite of the flue gas desulfurization gypsum produces large influence on portland cement setting time and strength, and through determination of calcium sulfite and calcium carbonate content, flue gas desulfurization gypsum slow setting effects on portland cement can be determined. The method promotes large scale use of flue gas desulfurization gypsum as industrial waste residue. The method has the advantages of simple determination method, accurate result and low determination cost.

The invention discloses a method and a kit for determination of human POT1 gene rs1034794 site polymorphism. The method is as follows: providing to-be-tested human genomic DNA; providing upstream and downstream primers for amplification of sequences nearby human POT1 gene rs1034794 site, wherein the upstream primer has a mismatched base A; taking the to-be-tested human genomic DNA as a template, using the upstream and downstream primers for PCR amplification reaction to obtain an amplification product containing AGCW fragment, wherein W is to be confirmed base A or T on the human POT1 gene rs1034794 site; providing a restriction endonuclease; using the restriction endonuclease for enzyme digestion of the amplification product to obtain a corresponding enzyme digestion product; and according to the resulting enzyme digestion product, determining whether the to be confirmed base W on the human POT1 gene rs1034794 site is A or T. The restriction endonuclease is restriction endonuclease which only be capable of cutting one of AGCA fragment or AGCT fragment. The determination method is fast and reliable, and can greatly reduce the determination cost.

This invention discloses one fluorescent capillary reaction device and it analysis method, wherein the device comprises fixing socket, capillary insert hole, laser emission window, fluorescent window and capillary. The method comprises the following steps: executing FCA method; putting the socket into fluorescent light path; inserting capillary into liquid to be tested into socket top; the laser passing socket emission window on capillary to generate fluorescent light from fixed socket emission window through single color device at tester.