The invention provides an anti-tritium glove and a preparation method thereof. Compound latex adopted for the anti-tritium glove is prepared from, by weight, 200 parts of butyl rubber emulsion, 0.10-0.25 part of polyoxyethylated castor oil, 0-5 parts of potassium hydroxide and 20 parts of dispersoid. The butyl rubber emulsion is prepared from, by weight, 160 parts of butyl rubber, 1.0-1.5 parts of oleic acid, 4.0-7.2 parts of potassium hydroxide, 5.5-6.4 parts of monopotassium phosphate, 35-50 parts of polyvinyl alcohol, 0.8-1.0 part of polyoxyethylene castor oil potassium soap and 94-100 parts of soft water. The dispersoid is prepared from, by weight, 4 parts of sulphur, 5 parts of accelerant Px, 10 parts of accelerant ZDC, 8 parts of zinc oxide, 2 parts of antiager DBH, 0.4 part of green pigment, 5 parts of dispersant NF, 0.8 part of stabilizer KOH, 0.1 part of carbon black, 1.5-5.0 parts of anti-tritium material and 50 parts of water. Compared with the prior art, the anti-tritium glove has the advantages that the tritium permeability coefficient, air tightness, mechanical performance, hot air aging resistance, sulfuric acid solution resistance and sodium hydroxide solution resistance are excellent, and the requirements of relevant military units are met.